WNK2 cDNA ORF clone, Gallus gallus(chicken)

The following WNK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WNK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa144021 XM_025154646.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa144014 XM_025154639.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa144015 XM_025154640.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa144016 XM_025154641.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa144017 XM_025154642.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa144018 XM_025154643.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa144019 XM_025154644.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa144020 XM_025154645.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa41672 XM_015293416.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41673 XM_015293417.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41674 XM_015293420.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41675 XM_015293421.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41676 XM_015293422.2
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41676 XM_015293423.2
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41677 XM_015293424.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41678 XM_015293425.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41679 XM_015293426.2
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41680 XM_015293427.2
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41681 XM_015293428.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41682 XM_015293429.1
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41683 XM_015293431.2
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41684 XM_015293432.2
Latest version!
Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OGa41679 XM_015293426.1 Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41676 XM_015293423.1 Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41676 XM_015293422.1 Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41683 XM_015293431.1 Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41684 XM_015293432.1 Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OGa41680 XM_015293427.1 Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa144021
    Clone ID Related Accession (Same CDS sequence) XM_025154646.1
    Accession Version XM_025154646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6000bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GGAGCAGGCA ATACAGGACA AACTTCAAAC TCTGTCACAG
    AGCTGTGAAA GCTACATGAG TCCAGATGTC GCTTCAGGTA AAGAGATGAG TGACAGCTTT
    GAAGGAGCAA TAGGAAGTGG AAAACAAGAA GGAAAGTCTT CAAAGAAACA TAAGAAATCT
    ACACGTGCTC GTTCACGCCA GGAGAAAACC AACAGACCCA AACTGACTAT ATTAAATGTT
    TGTAACACAG GGGATAAGAT GGTGGAGTGC CAGCTTGAAA CACACAATCA CAAAATGGTG
    ACTTTTAAGT TTGATTTGGA TGGTGATGCG CCTGAGGAAA TTGCTACTTA CATGGTAGAG
    AATGAATTCA TATTGCAGAG TGAGAAAGAG ACGTTTATTG AACAAATGAA AGATATTATC
    GATAAAGCAG AAGATATGCT CAGTGAAGAT ACAGAAGGAG AACGGAGTTC TGATCAGGGA
    ACAAGTCCCC AGCAGGATAC TGATGGAGCA GAAATGAGTG AGGAGAAGCG GCCATCTCAA
    GTGAAGACAC CCGTGTATCA ACAAAATGTT TTGCATACCG GAAAAAGGTG GTTTATTATA
    TGCCCTGTGG TGGAAAACCC GACTACTGAT GCACCAGAAT TTTCTCCACC AGTTCCTCAG
    AGTGCACAAC AGTCTGAAGG TCCAGACCTG GAGCAGTCGG ATGATCAGCA AGCAAACTCT
    GCGGTACCGG ATACGCCTGG TGTCTTAGCT TGTCAGCAGC TTCCTCTACA AGCAAGCGTC
    TGTGACAGCC CCATTGGGGC CTCTCCATCT TTGGCACAAA TTCCTGCTGC AGCATCTTCA
    GCTGGCTTGC CAAGCCAGGC AGAGCTCTCA GTTCCAGCAC CGCCAGGACC TGCTCCAAAC
    ATCCTGGAGC AGGCAGCCGC TAACGGAGGC CAGCCGGAAT GTTCTCTACT GAAAGCAGCA
    TTGCCCGCCC CACCTGGCAC TCCTGTCCCA GGTGCTGTCC CTCTGGAGGA GCCCGCTGAT
    GCTCCACTGG CTGCTCAGCC TACTCAGCAT GCGCAGCAGC AGGCAGACGA GGCCATGTGT
    ATCCCTGATG TGGAGATAGC GTGTTGCAGT GTCGGGCCAC CCAAAGCAGT CCCACCAGCT
    GCTGCCAAGG CACCGGAGCT CTGCCCGCTC CCTGTGCTCT CAGATGCTGT TGTTTTGGAG
    CGGCAGCCTG CGGCCCAGAT CCCTCACCTG CCAGAGGCCA GCCAGCCCGG CGTGGTGCAG
    CACTCCTTTG TGAACAAAGT GTTTCCTCCA TCTGTTGCAT CTGAGTCTCT CAATCTGGCA
    GGATCTCAAC TTCAGAGCCC CACTCAGGTG TCCATGGCCG CATCCCAGCA GCACGCGATG
    CTGTCCCAGA CACAGCCTGT CACCTGCGCC AGCGTGGGGA TCAGCCTGCT GCAGCAGCAG
    CAACAGCAGC AGCCGTGCAC CGTGGAGTCT GATGGAGAGG GGCCACCTCG GGTGGACTTT
    GTTGACAACA CAATAAAAAG CCTCGATGAG AAACTGCGCA ACTTACTTTA CCAGGAATAT
    GTCCCAACTT CTTCTGCATC AGCAGGAACT CCAGACGCTT TTGTGCCATT GGAACAGGCA
    GACAGTGAAT TTAACTTGCC ACCACTTACC GAAGATCAAG TTCCCAAGTT GGTGTTGGAT
    TTGAGAGAAC CAGGCCATAA CACCACGGAT AGCTGCCAGG AGGTGAAAGC TGCCGAGGCC
    CAGTTTGTGC CGGCTGTGCC ACCTGAGATC GCACCTTATC CAGCATTGCT TGAAAAGTCT
    GAGGTCGCTG GCATTGGGAC TGAGTCTTTG GAAGCAAGAG GTGGGTCAGC AAGCCAAAGA
    GGATCAGCTG CGAGCATCAC TCCAGCTCCT GTGACTCCAG CAAAGGGCCT CCTCCAGGTT
    GCACCTACAT CATCGAGCAT AGCTAAACAG ATGGAAACTT CTAAAACGAA CGAGAAGGCA
    AAGGCTACAA CTTCATCTAC GCACACAGAT AATGTAATGC AGCTATCGAC AGCAGATGGG
    GTGATAAATA ACCTTCAAAG GGATGACTCT CTAGACTCCG GTTCCTATGG AAAACAGGCT
    GGAACTCATG ATAGCGGTAC ACCAGCCAAA ACTATTGGCA GGTTTTCAGT AAGCAGCACG
    CAAGATGAAT TAACTTTAGC AGCGCCGCAT TGCTTAAGAT TCTCTGCGCC CCCGGACGTC
    TATTTGGATG AGCTCCCTTC CAGCCCCGAC CAGAAGACAG CTGTCCGACG GGTGCAGACG
    GCCTCGTCTG TTGATGTCCT TTGTGACCAG GGCTCGAGTG ATTCTGCCGA GGAGCCCTTC
    CATCGACAGG CTGCTGCGGC TCAGCCATCC CCTCCGAGCA GTAGTGCAGC CTCTGACTTG
    ATTAAAAAAG CAGCAGCTTT TCTGCAAAGG ACTGGAAAAG CAGGCTCCCA AGGCCCCGAT
    TCACCAAACG GGCAGGGAAC AAAGATTCCT ACCATCAACA TAACATCCTT CCACTCGCAG
    TCATCATACA TGAGCAGTGA CAATGACTCT GAATTTGAAG ATGCAGACAT GAAGAAAGAA
    CTGCAGAACT TGAGAGAGAA GCATATGAAA GAAATTGCTG AACTTCAGAC TCAGCAGAAG
    AATGAGATAG AGGCGCTGTA CATTCGGTTA GGGAAGCCCC TCCCTCCCAA CATGGGGTTC
    CTTCACTCAG CCCCGCCAAG CAGCCGTCGA CGAAGAACAA GCAAAAATAA ATTGAAAGGT
    GGAAAACTAT TAAACCCTCT GGTTCAACAG CTCAGGAGTG GAACATCAAG CACAAGTAAT
    GTTTCAGACT CTAAAGACTC ACCCCACAAA GAAACAACTA AAGCTCAGCC TGGATCTCCC
    GAAACAACAG GAGTAACTTC CTCTGTGTCA GCCACATTTG GGAAGTCTGT TCAGACACAG
    CAGCCATGCT CTGTCAAAGC CTCTTTGTCT TCTGACATCT GCTCCGGCTT AGGCCCCGAA
    GGAGGCGATG TGAATGCAAG CTCTGGCCAA GGCTGGACGG TTTTCCACCA AACGTCTGAG
    AGAGTGACCT ATAAATCTAG TAGCAAACCT CGTACCAGAT TCCTCAGTGG ACCTGTGTCT
    TTGTCCATCT GGTCCACCTT GAAACGACTA TGTCTAGGCA AAGATCACAG TAGTAGATCC
    TCTACAAACA GTCTTGTAAC GTGTCCTGAG CCCCTCCCGC AATCAACTCT GCAGGTTCAG
    GCCAACAACA GTAACAACAA GAAGGGAACG TTCACCGATG ATCTTCACAA GCTGGTTGAC
    CAGTGGACAA GCAAAACTGT TGGAGCTGCA CAGGCAAAAC CTTCTTTAAA CCAACTGAAG
    CAGAACCAAA AAAGGCAGGA CATGGAGCCA AAGGCAAATC CCATGCAAAC ACAGAATGAG
    ACATGTGGAG TTAATTCAGT AAGAACAGGA TTGGGGAAAA GGTGCATTGT TCCAGTGTCT
    TGTCCCATGT CAGCTGCGCT AACTCCTGGA GTTTCACCAT CCATGCCAGT GCCTATGCCA
    GTTTGCTCCA TAGGCAGCAC GGACACCTCT CTCCTGTTTG CGGGTTGCTT TTTGCCTCTT
    TTACTGTGCT TAGAGTCTGC ATCTGCACCT CTCAGTCTAA AGGCACAAGG TCACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010414.1
    CDS202..6201
    Translation

    Target ORF information:

    RefSeq Version XM_025154646.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GGAGCAGGCA ATACAGGACA AACTTCAAAC TCTGTCACAG
    AGCTGTGAAA GCTACATGAG TCCAGATGTC GCTTCAGGTA AAGAGATGAG TGACAGCTTT
    GAAGGAGCAA TAGGAAGTGG AAAACAAGAA GGAAAGTCTT CAAAGAAACA TAAGAAATCT
    ACACGTGCTC GTTCACGCCA GGAGAAAACC AACAGACCCA AACTGACTAT ATTAAATGTT
    TGTAACACAG GGGATAAGAT GGTGGAGTGC CAGCTTGAAA CACACAATCA CAAAATGGTG
    ACTTTTAAGT TTGATTTGGA TGGTGATGCG CCTGAGGAAA TTGCTACTTA CATGGTAGAG
    AATGAATTCA TATTGCAGAG TGAGAAAGAG ACGTTTATTG AACAAATGAA AGATATTATC
    GATAAAGCAG AAGATATGCT CAGTGAAGAT ACAGAAGGAG AACGGAGTTC TGATCAGGGA
    ACAAGTCCCC AGCAGGATAC TGATGGAGCA GAAATGAGTG AGGAGAAGCG GCCATCTCAA
    GTGAAGACAC CCGTGTATCA ACAAAATGTT TTGCATACCG GAAAAAGGTG GTTTATTATA
    TGCCCTGTGG TGGAAAACCC GACTACTGAT GCACCAGAAT TTTCTCCACC AGTTCCTCAG
    AGTGCACAAC AGTCTGAAGG TCCAGACCTG GAGCAGTCGG ATGATCAGCA AGCAAACTCT
    GCGGTACCGG ATACGCCTGG TGTCTTAGCT TGTCAGCAGC TTCCTCTACA AGCAAGCGTC
    TGTGACAGCC CCATTGGGGC CTCTCCATCT TTGGCACAAA TTCCTGCTGC AGCATCTTCA
    GCTGGCTTGC CAAGCCAGGC AGAGCTCTCA GTTCCAGCAC CGCCAGGACC TGCTCCAAAC
    ATCCTGGAGC AGGCAGCCGC TAACGGAGGC CAGCCGGAAT GTTCTCTACT GAAAGCAGCA
    TTGCCCGCCC CACCTGGCAC TCCTGTCCCA GGTGCTGTCC CTCTGGAGGA GCCCGCTGAT
    GCTCCACTGG CTGCTCAGCC TACTCAGCAT GCGCAGCAGC AGGCAGACGA GGCCATGTGT
    ATCCCTGATG TGGAGATAGC GTGTTGCAGT GTCGGGCCAC CCAAAGCAGT CCCACCAGCT
    GCTGCCAAGG CACCGGAGCT CTGCCCGCTC CCTGTGCTCT CAGATGCTGT TGTTTTGGAG
    CGGCAGCCTG CGGCCCAGAT CCCTCACCTG CCAGAGGCCA GCCAGCCCGG CGTGGTGCAG
    CACTCCTTTG TGAACAAAGT GTTTCCTCCA TCTGTTGCAT CTGAGTCTCT CAATCTGGCA
    GGATCTCAAC TTCAGAGCCC CACTCAGGTG TCCATGGCCG CATCCCAGCA GCACGCGATG
    CTGTCCCAGA CACAGCCTGT CACCTGCGCC AGCGTGGGGA TCAGCCTGCT GCAGCAGCAG
    CAACAGCAGC AGCCGTGCAC CGTGGAGTCT GATGGAGAGG GGCCACCTCG GGTGGACTTT
    GTTGACAACA CAATAAAAAG CCTCGATGAG AAACTGCGCA ACTTACTTTA CCAGGAATAT
    GTCCCAACTT CTTCTGCATC AGCAGGAACT CCAGACGCTT TTGTGCCATT GGAACAGGCA
    GACAGTGAAT TTAACTTGCC ACCACTTACC GAAGATCAAG TTCCCAAGTT GGTGTTGGAT
    TTGAGAGAAC CAGGCCATAA CACCACGGAT AGCTGCCAGG AGGTGAAAGC TGCCGAGGCC
    CAGTTTGTGC CGGCTGTGCC ACCTGAGATC GCACCTTATC CAGCATTGCT TGAAAAGTCT
    GAGGTCGCTG GCATTGGGAC TGAGTCTTTG GAAGCAAGAG GTGGGTCAGC AAGCCAAAGA
    GGATCAGCTG CGAGCATCAC TCCAGCTCCT GTGACTCCAG CAAAGGGCCT CCTCCAGGTT
    GCACCTACAT CATCGAGCAT AGCTAAACAG ATGGAAACTT CTAAAACGAA CGAGAAGGCA
    AAGGCTACAA CTTCATCTAC GCACACAGAT AATGTAATGC AGCTATCGAC AGCAGATGGG
    GTGATAAATA ACCTTCAAAG GGATGACTCT CTAGACTCCG GTTCCTATGG AAAACAGGCT
    GGAACTCATG ATAGCGGTAC ACCAGCCAAA ACTATTGGCA GGTTTTCAGT AAGCAGCACG
    CAAGATGAAT TAACTTTAGC AGCGCCGCAT TGCTTAAGAT TCTCTGCGCC CCCGGACGTC
    TATTTGGATG AGCTCCCTTC CAGCCCCGAC CAGAAGACAG CTGTCCGACG GGTGCAGACG
    GCCTCGTCTG TTGATGTCCT TTGTGACCAG GGCTCGAGTG ATTCTGCCGA GGAGCCCTTC
    CATCGACAGG CTGCTGCGGC TCAGCCATCC CCTCCGAGCA GTAGTGCAGC CTCTGACTTG
    ATTAAAAAAG CAGCAGCTTT TCTGCAAAGG ACTGGAAAAG CAGGCTCCCA AGGCCCCGAT
    TCACCAAACG GGCAGGGAAC AAAGATTCCT ACCATCAACA TAACATCCTT CCACTCGCAG
    TCATCATACA TGAGCAGTGA CAATGACTCT GAATTTGAAG ATGCAGACAT GAAGAAAGAA
    CTGCAGAACT TGAGAGAGAA GCATATGAAA GAAATTGCTG AACTTCAGAC TCAGCAGAAG
    AATGAGATAG AGGCGCTGTA CATTCGGTTA GGGAAGCCCC TCCCTCCCAA CATGGGGTTC
    CTTCACTCAG CCCCGCCAAG CAGCCGTCGA CGAAGAACAA GCAAAAATAA ATTGAAAGGT
    GGAAAACTAT TAAACCCTCT GGTTCAACAG CTCAGGAGTG GAACATCAAG CACAAGTAAT
    GTTTCAGACT CTAAAGACTC ACCCCACAAA GAAACAACTA AAGCTCAGCC TGGATCTCCC
    GAAACAACAG GAGTAACTTC CTCTGTGTCA GCCACATTTG GGAAGTCTGT TCAGACACAG
    CAGCCATGCT CTGTCAAAGC CTCTTTGTCT TCTGACATCT GCTCCGGCTT AGGCCCCGAA
    GGAGGCGATG TGAATGCAAG CTCTGGCCAA GGCTGGACGG TTTTCCACCA AACGTCTGAG
    AGAGTGACCT ATAAATCTAG TAGCAAACCT CGTACCAGAT TCCTCAGTGG ACCTGTGTCT
    TTGTCCATCT GGTCCACCTT GAAACGACTA TGTCTAGGCA AAGATCACAG TAGTAGATCC
    TCTACAAACA GTCTTGTAAC GTGTCCTGAG CCCCTCCCGC AATCAACTCT GCAGGTTCAG
    GCCAACAACA GTAACAACAA GAAGGGAACG TTCACCGATG ATCTTCACAA GCTGGTTGAC
    CAGTGGACAA GCAAAACTGT TGGAGCTGCA CAGGCAAAAC CTTCTTTAAA CCAACTGAAG
    CAGAACCAAA AAAGGCAGGA CATGGAGCCA AAGGCAAATC CCATGCAAAC ACAGAATGAG
    ACATGTGGAG TTAATTCAGT AAGAACAGGA TTGGGGAAAA GGTGCATTGT TCCAGTGTCT
    TGTCCCATGT CAGCTGCGCT AACTCCTGGA GTTTCACCAT CCATGCCAGT GCCTATGCCA
    GTTTGCTCCA TAGGCAGCAC GGACACCTCT CTCCTGTTTG CGGGTTGCTT TTTGCCTCTT
    TTACTGTGCT TAGAGTCTGC ATCTGCACCT CTCAGTCTAA AGGCACAAGG TCACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144014
    Clone ID Related Accession (Same CDS sequence) XM_025154639.1
    Accession Version XM_025154639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGTTACTGGA CATACTCAGT ATGGTTTGGA AACTGGAGCC
    CAGGTGCCGG TACTCCCAGT GCAGCCTTCG CTAGTCATGT CACCGCCTTT GCAGTTGCAA
    CCTGAACTAT TGCCTCCCCG CGTCGCTCCA GAAAGTATTT CACAGATTCA TGGCAGCCTT
    GCTACAGCTA GTCCACCAGT GCCCATAGAT CACTGTCTGC CTCTCCAGCC TTCGGGTGTT
    CTGCAGCTTC AGCCAGACCT TTCCCAGCCT CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA
    GCCGTGCAGG CAGTGGCAGA GACATCGCAA GAGGAGCAGG CAATACAGGA CAAACTTCAA
    ACTCTGTCAC AGAGCTGTGA AAGCTACATG AGTCCAGATG TCGCTTCAGG TAAAGAGATG
    AGTGACAGCT TTGAAGGAGC AATAGGAAGT GGAAAACAAG AAGGAAAGTC TTCAAAGAAA
    CATAAGAAAT CTACACGTGC TCGTTCACGC CAGGAGAAAA CCAACAGACC CAAACTGACT
    ATATTAAATG TTTGTAACAC AGGGGATAAG ATGGTGGAGT GCCAGCTTGA AACACACAAT
    CACAAAATGG TGACTTTTAA GTTTGATTTG GATGGTGATG CGCCTGAGGA AATTGCTACT
    TACATGGTAG AGAATGAATT CATATTGCAG AGTGAGAAAG AGACGTTTAT TGAACAAATG
    AAAGATATTA TCGATAAAGC AGAAGATATG CTCAGTGAAG ATACAGAAGG AGAACGGAGT
    TCTGATCAGG GAACAAGTCC CCAGCAGGAT ACTGATGGAG CAGAAATGAG TGAGGAGAAG
    CGGCCATCTC AAGTGAAGAC ACCCGTGTAT CAACAAAATG TTTTGCATAC CGGAAAAAGG
    TGGTTTATTA TATGCCCTGT GGTGGAAAAC CCGACTACTG ATGCACCAGA ATTTTCTCCA
    CCAGTTCCTC AGAGTGCACA ACAGTCTGAA GGTCCAGACC TGGAGCAGTC GGATGATCAG
    CAAGCAAACT CTGCGGTACC GGATACGCCT GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA
    CAAGCAAGCG TCTGTGACAG CCCCATTGGG GCCTCTCCAT CTTTGGCACA AATTCCTGCT
    GCAGCATCTT CAGCTGGCTT GCCAAGCCAG GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA
    CCTGCTCCAA ACATCCTGGA GCAGGCAGCC GCTAACGGAG GCCAGCCGGA ATGTTCTCTA
    CTGAAAGCAG CATTGCCCGC CCCACCTGGC ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG
    GAGCCCGCTG ATGCTCCACT GGCTGCTCAG CCTACTCAGC ATGCGCAGCA GCAGGCAGAC
    GAGGCCATGT GTATCCCTGA TGTGGAGATA GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA
    GTCCCACCAG CTGCTGCCAA GGCACCGGAG CTCTGCCCGC TCCCTGTGCT CTCAGATGCT
    GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG ATCCCTCACC TGCCAGAGGC CAGCCAGCCC
    GGCGTGGTGC AGCACTCCTT TGTGAACAAA GTGTTTCCTC CATCTGTTGC ATCTGAGTCT
    CTCAATCTGG CAGGATCTCA ACTTCAGAGC CCCACTCAGG TGTCCATGGC CGCATCCCAG
    CAGCACGCGA TGCTGTCCCA GACACAGCCT GTCACCTGCG CCAGCGTGGG GATCAGCCTG
    CTGCAGCAGC AGCAACAGCA GCAGCCGTGC ACCGTGGAGT CTGATGGAGA GGGGCCACCT
    CGGGTGGACT TTGTTGACAA CACAATAAAA AGCCTCGATG AGAAACTGCG CAACTTACTT
    TACCAGGAAT ATGTCCCAAC TTCTTCTGCA TCAGCAGGAA CTCCAGACGC TTTTGTGCCA
    TTGGAACAGG CAGACAGTGA ATTTAACTTG CCACCACTTA CCGAAGATCA AGTTCCCAAG
    TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT AACACCACGG ATAGCTGCCA GGAGGTGAAA
    GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG CCACCTGAGA TCGCACCTTA TCCAGCATTG
    CTTGAAAAGT CTGAGGTCGC TGGCATTGGG ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA
    GCAAGCCAAA GAGGATCAGC TGCGAGCATC ACTCCAGCTC CTGTGACTCC AGCAAAGGGC
    CTCCTCCAGG TTGCACCTAC ATCATCGAGC ATAGCTAAAC AGATGGAAAC TTCTAAAACG
    AACGAGAAGG CAAAGGCTAC AACTTCATCT ACGCACACAG ATAATGTAAT GCAGCTATCG
    ACAGCAGATG GGGTGATAAA TAACCTTCAA AGGGATGACT CTCTAGACTC CGGTTCCTAT
    GGAAAACAGG CTGGAACTCA TGATAGCGGT ACACCAGCCA AAACTATTGG CAGGTTTTCA
    GTAAGCAGCA CGCAAGATGA ATTAACTTTA GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG
    CCCCCGGACG TCTATTTGGA TGAGCTCCCT TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA
    CGGGTGCAGA CGGCCTCGTC TGTTGATGTC CTTTGTGACC AGGGCTCGAG TGATTCTGCC
    GAGGAGCCCT TCCATCGACA GGCTGCTGCG GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA
    GCCTCTGACT TGATTAAAAA AGCAGCAGCT TTTCTGCAAA GGACTGGAAA AGCAGGCTCC
    CAAGGCCCCG ATTCACCAAA CGGGCAGGGA ACAAAGATTC CTACCATCAA CATAACATCC
    TTCCACTCGC AGTCATCATA CATGAGCAGT GACAATGACT CTGAATTTGA AGATGCAGAC
    ATGAAGAAAG AACTGCAGAA CTTGAGAGAG AAGCATATGA AAGAAATTGC TGAACTTCAG
    ACTCAGCAGA AGAATGAGAT AGAGGCGCTG TACATTCGGT TAGGGAAGCC CCTCCCTCCC
    AACATGGGGT TCCTTCACTC AGCCCCGCCA AGCAGCCGTC GACGAAGAAC AAGCAAAAAT
    AAATTGAAAG GTGGAAAACT ATTAAACCCT CTGGTTCAAC AGCTCAGGAG TGGAACATCA
    AGCACAAGTA ATGTTTCAGA CTCTAAAGAC TCACCCCACA AAGAAACAAC TAAAGCTCAG
    CCTGGATCTC CCGAAACAAC AGGAGTAACT TCCTCTGTGT CAGCCACATT TGGGAAGTCT
    GTTCAGACAC AGCAGCCATG CTCTGTCAAA GCCTCTTTGT CTTCTGACAT CTGCTCCGGC
    TTAGGCCCCG AAGGAGGCGA TGTGAATGCA AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC
    CAAACGTCTG AGAGAGTGAC CTATAAATCT AGTAGCAAAC CTCGTACCAG ATTCCTCAGT
    GGACCTGTGT CTTTGTCCAT CTGGTCCACC TTGAAACGAC TATGTCTAGG CAAAGATCAC
    AGTAGTAGAT CCTCTACAAA CAGTCTTGTA ACGTGTCCTG AGCCCCTCCC GCAATCAACT
    CTGCAGGTTC AGGCCAACAA CAGTAACAAC AAGAAGGGAA CGTTCACCGA TGATCTTCAC
    AAGCTGGTTG ACCAGTGGAC AAGCAAAACT GTTGGAGCTG CACAGGCAAA ACCTTCTTTA
    AACCAACTGA AGCAGAACCA AAAAAGGCAG GACATGGAGC CAAAGGCAAA TCCCATGCAA
    ACACAGAATG AGACATGTGG AGTTAATTCA GTAAGAACAG GATTGGGGAA AAGGTGCATT
    GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG CTAACTCCTG GAGTTTCACC ATCCATGCCA
    GTGCCTATGC CAGTTTGCTC CATAGGCAGC ACGGACACCT CTCTCCTGTT TGCGGGTTGC
    TTTTTGCCTC TTTTACTGTG CTTAGAGTCT GCATCTGCAC CTCTCAGTCT AAAGGCACAA
    GGTCACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010407.1
    CDS203..7054
    Translation

    Target ORF information:

    RefSeq Version XM_025154639.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGTTACTGGA CATACTCAGT ATGGTTTGGA AACTGGAGCC
    CAGGTGCCGG TACTCCCAGT GCAGCCTTCG CTAGTCATGT CACCGCCTTT GCAGTTGCAA
    CCTGAACTAT TGCCTCCCCG CGTCGCTCCA GAAAGTATTT CACAGATTCA TGGCAGCCTT
    GCTACAGCTA GTCCACCAGT GCCCATAGAT CACTGTCTGC CTCTCCAGCC TTCGGGTGTT
    CTGCAGCTTC AGCCAGACCT TTCCCAGCCT CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA
    GCCGTGCAGG CAGTGGCAGA GACATCGCAA GAGGAGCAGG CAATACAGGA CAAACTTCAA
    ACTCTGTCAC AGAGCTGTGA AAGCTACATG AGTCCAGATG TCGCTTCAGG TAAAGAGATG
    AGTGACAGCT TTGAAGGAGC AATAGGAAGT GGAAAACAAG AAGGAAAGTC TTCAAAGAAA
    CATAAGAAAT CTACACGTGC TCGTTCACGC CAGGAGAAAA CCAACAGACC CAAACTGACT
    ATATTAAATG TTTGTAACAC AGGGGATAAG ATGGTGGAGT GCCAGCTTGA AACACACAAT
    CACAAAATGG TGACTTTTAA GTTTGATTTG GATGGTGATG CGCCTGAGGA AATTGCTACT
    TACATGGTAG AGAATGAATT CATATTGCAG AGTGAGAAAG AGACGTTTAT TGAACAAATG
    AAAGATATTA TCGATAAAGC AGAAGATATG CTCAGTGAAG ATACAGAAGG AGAACGGAGT
    TCTGATCAGG GAACAAGTCC CCAGCAGGAT ACTGATGGAG CAGAAATGAG TGAGGAGAAG
    CGGCCATCTC AAGTGAAGAC ACCCGTGTAT CAACAAAATG TTTTGCATAC CGGAAAAAGG
    TGGTTTATTA TATGCCCTGT GGTGGAAAAC CCGACTACTG ATGCACCAGA ATTTTCTCCA
    CCAGTTCCTC AGAGTGCACA ACAGTCTGAA GGTCCAGACC TGGAGCAGTC GGATGATCAG
    CAAGCAAACT CTGCGGTACC GGATACGCCT GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA
    CAAGCAAGCG TCTGTGACAG CCCCATTGGG GCCTCTCCAT CTTTGGCACA AATTCCTGCT
    GCAGCATCTT CAGCTGGCTT GCCAAGCCAG GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA
    CCTGCTCCAA ACATCCTGGA GCAGGCAGCC GCTAACGGAG GCCAGCCGGA ATGTTCTCTA
    CTGAAAGCAG CATTGCCCGC CCCACCTGGC ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG
    GAGCCCGCTG ATGCTCCACT GGCTGCTCAG CCTACTCAGC ATGCGCAGCA GCAGGCAGAC
    GAGGCCATGT GTATCCCTGA TGTGGAGATA GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA
    GTCCCACCAG CTGCTGCCAA GGCACCGGAG CTCTGCCCGC TCCCTGTGCT CTCAGATGCT
    GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG ATCCCTCACC TGCCAGAGGC CAGCCAGCCC
    GGCGTGGTGC AGCACTCCTT TGTGAACAAA GTGTTTCCTC CATCTGTTGC ATCTGAGTCT
    CTCAATCTGG CAGGATCTCA ACTTCAGAGC CCCACTCAGG TGTCCATGGC CGCATCCCAG
    CAGCACGCGA TGCTGTCCCA GACACAGCCT GTCACCTGCG CCAGCGTGGG GATCAGCCTG
    CTGCAGCAGC AGCAACAGCA GCAGCCGTGC ACCGTGGAGT CTGATGGAGA GGGGCCACCT
    CGGGTGGACT TTGTTGACAA CACAATAAAA AGCCTCGATG AGAAACTGCG CAACTTACTT
    TACCAGGAAT ATGTCCCAAC TTCTTCTGCA TCAGCAGGAA CTCCAGACGC TTTTGTGCCA
    TTGGAACAGG CAGACAGTGA ATTTAACTTG CCACCACTTA CCGAAGATCA AGTTCCCAAG
    TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT AACACCACGG ATAGCTGCCA GGAGGTGAAA
    GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG CCACCTGAGA TCGCACCTTA TCCAGCATTG
    CTTGAAAAGT CTGAGGTCGC TGGCATTGGG ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA
    GCAAGCCAAA GAGGATCAGC TGCGAGCATC ACTCCAGCTC CTGTGACTCC AGCAAAGGGC
    CTCCTCCAGG TTGCACCTAC ATCATCGAGC ATAGCTAAAC AGATGGAAAC TTCTAAAACG
    AACGAGAAGG CAAAGGCTAC AACTTCATCT ACGCACACAG ATAATGTAAT GCAGCTATCG
    ACAGCAGATG GGGTGATAAA TAACCTTCAA AGGGATGACT CTCTAGACTC CGGTTCCTAT
    GGAAAACAGG CTGGAACTCA TGATAGCGGT ACACCAGCCA AAACTATTGG CAGGTTTTCA
    GTAAGCAGCA CGCAAGATGA ATTAACTTTA GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG
    CCCCCGGACG TCTATTTGGA TGAGCTCCCT TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA
    CGGGTGCAGA CGGCCTCGTC TGTTGATGTC CTTTGTGACC AGGGCTCGAG TGATTCTGCC
    GAGGAGCCCT TCCATCGACA GGCTGCTGCG GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA
    GCCTCTGACT TGATTAAAAA AGCAGCAGCT TTTCTGCAAA GGACTGGAAA AGCAGGCTCC
    CAAGGCCCCG ATTCACCAAA CGGGCAGGGA ACAAAGATTC CTACCATCAA CATAACATCC
    TTCCACTCGC AGTCATCATA CATGAGCAGT GACAATGACT CTGAATTTGA AGATGCAGAC
    ATGAAGAAAG AACTGCAGAA CTTGAGAGAG AAGCATATGA AAGAAATTGC TGAACTTCAG
    ACTCAGCAGA AGAATGAGAT AGAGGCGCTG TACATTCGGT TAGGGAAGCC CCTCCCTCCC
    AACATGGGGT TCCTTCACTC AGCCCCGCCA AGCAGCCGTC GACGAAGAAC AAGCAAAAAT
    AAATTGAAAG GTGGAAAACT ATTAAACCCT CTGGTTCAAC AGCTCAGGAG TGGAACATCA
    AGCACAAGTA ATGTTTCAGA CTCTAAAGAC TCACCCCACA AAGAAACAAC TAAAGCTCAG
    CCTGGATCTC CCGAAACAAC AGGAGTAACT TCCTCTGTGT CAGCCACATT TGGGAAGTCT
    GTTCAGACAC AGCAGCCATG CTCTGTCAAA GCCTCTTTGT CTTCTGACAT CTGCTCCGGC
    TTAGGCCCCG AAGGAGGCGA TGTGAATGCA AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC
    CAAACGTCTG AGAGAGTGAC CTATAAATCT AGTAGCAAAC CTCGTACCAG ATTCCTCAGT
    GGACCTGTGT CTTTGTCCAT CTGGTCCACC TTGAAACGAC TATGTCTAGG CAAAGATCAC
    AGTAGTAGAT CCTCTACAAA CAGTCTTGTA ACGTGTCCTG AGCCCCTCCC GCAATCAACT
    CTGCAGGTTC AGGCCAACAA CAGTAACAAC AAGAAGGGAA CGTTCACCGA TGATCTTCAC
    AAGCTGGTTG ACCAGTGGAC AAGCAAAACT GTTGGAGCTG CACAGGCAAA ACCTTCTTTA
    AACCAACTGA AGCAGAACCA AAAAAGGCAG GACATGGAGC CAAAGGCAAA TCCCATGCAA
    ACACAGAATG AGACATGTGG AGTTAATTCA GTAAGAACAG GATTGGGGAA AAGGTGCATT
    GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG CTAACTCCTG GAGTTTCACC ATCCATGCCA
    GTGCCTATGC CAGTTTGCTC CATAGGCAGC ACGGACACCT CTCTCCTGTT TGCGGGTTGC
    TTTTTGCCTC TTTTACTGTG CTTAGAGTCT GCATCTGCAC CTCTCAGTCT AAAGGCACAA
    GGTCACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144015
    Clone ID Related Accession (Same CDS sequence) XM_025154640.1
    Accession Version XM_025154640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGATCA
    GCTGCGAGCA TCACTCCAGC TCCTGTGACT CCAGCAAAGG GCCTCCTCCA GGTTGCACCT
    ACATCATCGA GCATAGCTAA ACAGATGGAA ACTTCTAAAA CGAACGAGAA GGCAAAGGCT
    ACAACTTCAT CTACGCACAC AGATAATGTA ATGCAGCTAT CGACAGCAGA TGGGGTGATA
    AATAACCTTC AAAGGGATGA CTCTCTAGAC TCCGGTTCCT ATGGAAAACA GGCTGGAACT
    CATGATAGCG GTACACCAGC CAAAACTATT GGCAGGTTTT CAGTAAGCAG CACGCAAGAT
    GAATTAACTT TAGCAGCGCC GCATTGCTTA AGATTCTCTG CGCCCCCGGA CGTCTATTTG
    GATGAGCTCC CTTCCAGCCC CGACCAGAAG ACAGCTGTCC GACGGGTGCA GACGGCCTCG
    TCTGTTGATG TCCTTTGTGA CCAGGGCTCG AGTGATTCTG CCGAGGAGCC CTTCCATCGA
    CAGGCTGCTG CGGCTCAGCC ATCCCCTCCG AGCAGTAGTG CAGCCTCTGA CTTGATTAAA
    AAAGCAGCAG CTTTTCTGCA AAGGACTGGA AAAGCAGGCT CCCAAGGCCC CGATTCACCA
    AACGGGCAGG GAACAAAGAT TCCTACCATC AACATAACAT CCTTCCACTC GCAGTCATCA
    TACATGAGCA GTGACAATGA CTCTGAATTT GAAGATGCAG ACATGAAGAA AGAACTGCAG
    AACTTGAGAG AGAAGCATAT GAAAGAAATT GCTGAACTTC AGACTCAGCA GAAGAATGAG
    ATAGAGGCGC TGTACATTCG GTTAGGGAAG CCCCTCCCTC CCAACATGGG GTTCCTTCAC
    TCAGCCCCGC CAAGCAGCCG TCGACGAAGA ACAAGCAAAA ATAAATTGAA AGGTGGAAAA
    CTATTAAACC CTCTGGTTCA ACAGCTCAGG AGTGGAACAT CAAGCACAAG TAATGTTTCA
    GACTCTAAAG ACTCACCCCA CAAAGAAACA ACTAAAGCTC AGCCTGGATC TCCCGAAACA
    ACAGGAGTAA CTTCCTCTGT GTCAGCCACA TTTGGGAAGT CTGTTCAGAC ACAGCAGCCA
    TGCTCTGTCA AAGCCTCTTT GTCTTCTGAC ATCTGCTCCG GCTTAGGCCC CGAAGGAGGC
    GATGTGAATG CAAGCTCTGG CCAAGGCTGG ACGGTTTTCC ACCAAACGTC TGAGAGAGTG
    ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTTTGTCC
    ATCTGGTCCA CCTTGAAACG ACTATGTCTA GGCAAAGATC ACAGTAGTAG ATCCTCTACA
    AACAGTCTTG TAACGTGTCC TGAGCCCCTC CCGCAATCAA CTCTGCAGGT TCAGGCCAAC
    AACAGTAACA ACAAGAAGGG AACGTTCACC GATGATCTTC ACAAGCTGGT TGACCAGTGG
    ACAAGCAAAA CTGTTGGAGC TGCACAGGCA AAACCTTCTT TAAACCAACT GAAGCAGAAC
    CAAAAAAGGC AGGACATGGA GCCAAAGGCA AATCCCATGC AAACACAGAA TGAGACATGT
    GGAGTTAATT CAGTAAGAAC AGGATTGGGG AAAAGGTGCA TTGTTCCAGT GTCTTGTCCC
    ATGTCAGCTG CGCTAACTCC TGGAGTTTCA CCATCCATGC CAGTGCCTAT GCCAGTTTGC
    TCCATAGGCA GCACGGACAC CTCTCTCCTG TTTGCGGGTT GCTTTTTGCC TCTTTTACTG
    TGCTTAGAGT CTGCATCTGC ACCTCTCAGT CTAAAGGCAC AAGGTCACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010408.1
    CDS202..7035
    Translation

    Target ORF information:

    RefSeq Version XM_025154640.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGATCA
    GCTGCGAGCA TCACTCCAGC TCCTGTGACT CCAGCAAAGG GCCTCCTCCA GGTTGCACCT
    ACATCATCGA GCATAGCTAA ACAGATGGAA ACTTCTAAAA CGAACGAGAA GGCAAAGGCT
    ACAACTTCAT CTACGCACAC AGATAATGTA ATGCAGCTAT CGACAGCAGA TGGGGTGATA
    AATAACCTTC AAAGGGATGA CTCTCTAGAC TCCGGTTCCT ATGGAAAACA GGCTGGAACT
    CATGATAGCG GTACACCAGC CAAAACTATT GGCAGGTTTT CAGTAAGCAG CACGCAAGAT
    GAATTAACTT TAGCAGCGCC GCATTGCTTA AGATTCTCTG CGCCCCCGGA CGTCTATTTG
    GATGAGCTCC CTTCCAGCCC CGACCAGAAG ACAGCTGTCC GACGGGTGCA GACGGCCTCG
    TCTGTTGATG TCCTTTGTGA CCAGGGCTCG AGTGATTCTG CCGAGGAGCC CTTCCATCGA
    CAGGCTGCTG CGGCTCAGCC ATCCCCTCCG AGCAGTAGTG CAGCCTCTGA CTTGATTAAA
    AAAGCAGCAG CTTTTCTGCA AAGGACTGGA AAAGCAGGCT CCCAAGGCCC CGATTCACCA
    AACGGGCAGG GAACAAAGAT TCCTACCATC AACATAACAT CCTTCCACTC GCAGTCATCA
    TACATGAGCA GTGACAATGA CTCTGAATTT GAAGATGCAG ACATGAAGAA AGAACTGCAG
    AACTTGAGAG AGAAGCATAT GAAAGAAATT GCTGAACTTC AGACTCAGCA GAAGAATGAG
    ATAGAGGCGC TGTACATTCG GTTAGGGAAG CCCCTCCCTC CCAACATGGG GTTCCTTCAC
    TCAGCCCCGC CAAGCAGCCG TCGACGAAGA ACAAGCAAAA ATAAATTGAA AGGTGGAAAA
    CTATTAAACC CTCTGGTTCA ACAGCTCAGG AGTGGAACAT CAAGCACAAG TAATGTTTCA
    GACTCTAAAG ACTCACCCCA CAAAGAAACA ACTAAAGCTC AGCCTGGATC TCCCGAAACA
    ACAGGAGTAA CTTCCTCTGT GTCAGCCACA TTTGGGAAGT CTGTTCAGAC ACAGCAGCCA
    TGCTCTGTCA AAGCCTCTTT GTCTTCTGAC ATCTGCTCCG GCTTAGGCCC CGAAGGAGGC
    GATGTGAATG CAAGCTCTGG CCAAGGCTGG ACGGTTTTCC ACCAAACGTC TGAGAGAGTG
    ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTTTGTCC
    ATCTGGTCCA CCTTGAAACG ACTATGTCTA GGCAAAGATC ACAGTAGTAG ATCCTCTACA
    AACAGTCTTG TAACGTGTCC TGAGCCCCTC CCGCAATCAA CTCTGCAGGT TCAGGCCAAC
    AACAGTAACA ACAAGAAGGG AACGTTCACC GATGATCTTC ACAAGCTGGT TGACCAGTGG
    ACAAGCAAAA CTGTTGGAGC TGCACAGGCA AAACCTTCTT TAAACCAACT GAAGCAGAAC
    CAAAAAAGGC AGGACATGGA GCCAAAGGCA AATCCCATGC AAACACAGAA TGAGACATGT
    GGAGTTAATT CAGTAAGAAC AGGATTGGGG AAAAGGTGCA TTGTTCCAGT GTCTTGTCCC
    ATGTCAGCTG CGCTAACTCC TGGAGTTTCA CCATCCATGC CAGTGCCTAT GCCAGTTTGC
    TCCATAGGCA GCACGGACAC CTCTCTCCTG TTTGCGGGTT GCTTTTTGCC TCTTTTACTG
    TGCTTAGAGT CTGCATCTGC ACCTCTCAGT CTAAAGGCAC AAGGTCACCA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144016
    Clone ID Related Accession (Same CDS sequence) XM_025154641.1
    Accession Version XM_025154641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGGTC CACCTTGAAA
    CGACTATGTC TAGGCAAAGA TCACAGTAGT AGATCCTCTA CAAACAGTCT TGTAACGTGT
    CCTGAGCCCC TCCCGCAATC AACTCTGCAG GTTCAGGCCA ACAACAGTAA CAACAAGAAG
    GGAACGTTCA CCGATGATCT TCACAAGCTG GTTGACCAGT GGACAAGCAA AACTGTTGGA
    GCTGCACAGG CAAAACCTTC TTTAAACCAA CTGAAGCAGA ACCAAAAAAG GCAGGACATG
    GAGCCAAAGG CAAATCCCAT GCAAACACAG AATGAGACAT GTGGAGTTAA TTCAGTAAGA
    ACAGGATTGG GGAAAAGGTG CATTGTTCCA GTGTCTTGTC CCATGTCAGC TGCGCTAACT
    CCTGGAGTTT CACCATCCAT GCCAGTGCCT ATGCCAGTTT GCTCCATAGG CAGCACGGAC
    ACCTCTCTCC TGTTTGCGGG TTGCTTTTTG CCTCTTTTAC TGTGCTTAGA GTCTGCATCT
    GCACCTCTCA GTCTAAAGGC ACAAGGTCAC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010409.1
    CDS202..6957
    Translation

    Target ORF information:

    RefSeq Version XM_025154641.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGGTC CACCTTGAAA
    CGACTATGTC TAGGCAAAGA TCACAGTAGT AGATCCTCTA CAAACAGTCT TGTAACGTGT
    CCTGAGCCCC TCCCGCAATC AACTCTGCAG GTTCAGGCCA ACAACAGTAA CAACAAGAAG
    GGAACGTTCA CCGATGATCT TCACAAGCTG GTTGACCAGT GGACAAGCAA AACTGTTGGA
    GCTGCACAGG CAAAACCTTC TTTAAACCAA CTGAAGCAGA ACCAAAAAAG GCAGGACATG
    GAGCCAAAGG CAAATCCCAT GCAAACACAG AATGAGACAT GTGGAGTTAA TTCAGTAAGA
    ACAGGATTGG GGAAAAGGTG CATTGTTCCA GTGTCTTGTC CCATGTCAGC TGCGCTAACT
    CCTGGAGTTT CACCATCCAT GCCAGTGCCT ATGCCAGTTT GCTCCATAGG CAGCACGGAC
    ACCTCTCTCC TGTTTGCGGG TTGCTTTTTG CCTCTTTTAC TGTGCTTAGA GTCTGCATCT
    GCACCTCTCA GTCTAAAGGC ACAAGGTCAC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144017
    Clone ID Related Accession (Same CDS sequence) XM_025154642.1
    Accession Version XM_025154642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    GTTAATTCAG TAAGAACAGG ATTGGGGAAA AGGTGCATTG TTCCAGTGTC TTGTCCCATG
    TCAGCTGCGC TAACTCCTGG AGTTTCACCA TCCATGCCAG TGCCTATGCC AGTTTGCTCC
    ATAGGCAGCA CGGACACCTC TCTCCTGTTT GCGGGTTGCT TTTTGCCTCT TTTACTGTGC
    TTAGAGTCTG CATCTGCACC TCTCAGTCTA AAGGCACAAG GTCACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010410.1
    CDS202..6912
    Translation

    Target ORF information:

    RefSeq Version XM_025154642.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    GTTAATTCAG TAAGAACAGG ATTGGGGAAA AGGTGCATTG TTCCAGTGTC TTGTCCCATG
    TCAGCTGCGC TAACTCCTGG AGTTTCACCA TCCATGCCAG TGCCTATGCC AGTTTGCTCC
    ATAGGCAGCA CGGACACCTC TCTCCTGTTT GCGGGTTGCT TTTTGCCTCT TTTACTGTGC
    TTAGAGTCTG CATCTGCACC TCTCAGTCTA AAGGCACAAG GTCACCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144018
    Clone ID Related Accession (Same CDS sequence) XM_025154643.1
    Accession Version XM_025154643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC AACCCATATC ACAGCAAACT
    TCTCTGCAGC AGGTGCTTGC CAGCCAGCCG GTTTGCCCCA TCCAGCCTGC CCCCCATCTC
    CTGCCACAGT ATCAGCCCCA GACCTCTCAG GTGGCAGCCA GTAGTACACC ACTGAAACCC
    CTTCAGATTT CAACTGTGCC ACAGCTTCCT CCAGTTGCCC CTTCCCAGAT TCCTCAGTTT
    CCCGTCATTC CTGCAATTAC TCCTCTGGCA GGACTTGACA ACCTTCCTCC AAACCTCTCT
    GATATACCTG CTGCAAACGT GCCCCCCATA CCTGCTCCTT CTCAGTATTT TGCTCCAGCT
    GTGATTTTAC CGAGCCTCCC CATGAACCCC ACTTTGCCCA TGGCACCAAA CTCCCCTGCC
    CTCCCCATGC AAGCAGTGAA CCTTCCTCAT ACAACTGTGG CTTCTCTGGT CTTGCCCTGC
    CAAACAATAG TGCCAAATAT GTCGGCTGCT ACAATACCGT TGCTTGCTGT GGCTCCGCCG
    GGAGTGCCAG CGCTGCCGCC GCACCACGCG GTGTCCCAGC TGCCCCAGCA GCAGATGTAC
    CCGACCACCT TCCAGCAGAT AATCCAGAGC GAAGCGCCCT CTCCCCATCA CACGCAGAGC
    ACGCAAGCCG TTTCCCAGCC GCAGCAGCCT CCACCTCCTC AGTCACTTCA GCCAGGCGTA
    ATTCATCCAT CAGAACAGCC GTTGTCCGCC CCTGGGGCTG GTAACCAGCA GGTTACTGGA
    CATACTCAGT ATGGTTTGGA AACTGGAGCC CAGGTGCCGG TACTCCCAGT GCAGCCTTCG
    CTAGTCATGT CACCGCCTTT GCAGTTGCAA CCTGAACTAT TGCCTCCCCG CGTCGCTCCA
    GAAAGTATTT CACAGATTCA TGGCAGCCTT GCTACAGCTA GTCCACCAGT GCCCATAGAT
    CACTGTCTGC CTCTCCAGCC TTCGGGTGTT CTGCAGCTTC AGCCAGACCT TTCCCAGCCT
    CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA GCCGTGCAGG CAGTGGCAGA GACATCGCAA
    GAGGAGCAGG CAATACAGGA CAAACTTCAA ACTCTGTCAC AGAGCTGTGA AAGCTACATG
    AGTCCAGATG TCGCTTCAGG TAAAGAGATG AGTGACAGCT TTGAAGGAGC AATAGGAAGT
    GGAAAACAAG AAGGAAAGTC TTCAAAGAAA CATAAGAAAT CTACACGTGC TCGTTCACGC
    CAGGAGAAAA CCAACAGACC CAAACTGACT ATATTAAATG TTTGTAACAC AGGGGATAAG
    ATGGTGGAGT GCCAGCTTGA AACACACAAT CACAAAATGG TGACTTTTAA GTTTGATTTG
    GATGGTGATG CGCCTGAGGA AATTGCTACT TACATGGTAG AGAATGAATT CATATTGCAG
    AGTGAGAAAG AGACGTTTAT TGAACAAATG AAAGATATTA TCGATAAAGC AGAAGATATG
    CTCAGTGAAG ATACAGAAGG AGAACGGAGT TCTGATCAGG GAACAAGTCC CCAGCAGGAT
    ACTGATGGAG CAGAAATGAG TGAGGAGAAG CGGCCATCTC AAGTGAAGAC ACCCGTGTAT
    CAACAAAATG TTTTGCATAC CGGAAAAAGG TGGTTTATTA TATGCCCTGT GGTGGAAAAC
    CCGACTACTG ATGCACCAGA ATTTTCTCCA CCAGTTCCTC AGAGTGCACA ACAGTCTGAA
    GGTCCAGACC TGGAGCAGTC GGATGATCAG CAAGCAAACT CTGCGGTACC GGATACGCCT
    GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA CAAGCAAGCG TCTGTGACAG CCCCATTGGG
    GCCTCTCCAT CTTTGGCACA AATTCCTGCT GCAGCATCTT CAGCTGGCTT GCCAAGCCAG
    GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA CCTGCTCCAA ACATCCTGGA GCAGGCAGCC
    GCTAACGGAG GCCAGCCGGA ATGTTCTCTA CTGAAAGCAG CATTGCCCGC CCCACCTGGC
    ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG GAGCCCGCTG ATGCTCCACT GGCTGCTCAG
    CCTACTCAGC ATGCGCAGCA GCAGGCAGAC GAGGCCATGT GTATCCCTGA TGTGGAGATA
    GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA GTCCCACCAG CTGCTGCCAA GGCACCGGAG
    CTCTGCCCGC TCCCTGTGCT CTCAGATGCT GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG
    ATCCCTCACC TGCCAGAGGC CAGCCAGCCC GGCGTGGTGC AGCACTCCTT TGTGAACAAA
    GTGTTTCCTC CATCTGTTGC ATCTGAGTCT CTCAATCTGG CAGGATCTCA ACTTCAGAGC
    CCCACTCAGG TGTCCATGGC CGCATCCCAG CAGCACGCGA TGCTGTCCCA GACACAGCCT
    GTCACCTGCG CCAGCGTGGG GATCAGCCTG CTGCAGCAGC AGCAACAGCA GCAGCCGTGC
    ACCGTGGAGT CTGATGGAGA GGGGCCACCT CGGGTGGACT TTGTTGACAA CACAATAAAA
    AGCCTCGATG AGAAACTGCG CAACTTACTT TACCAGGAAT ATGTCCCAAC TTCTTCTGCA
    TCAGCAGGAA CTCCAGACGC TTTTGTGCCA TTGGAACAGG CAGACAGTGA ATTTAACTTG
    CCACCACTTA CCGAAGATCA AGTTCCCAAG TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT
    AACACCACGG ATAGCTGCCA GGAGGTGAAA GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG
    CCACCTGAGA TCGCACCTTA TCCAGCATTG CTTGAAAAGT CTGAGGTCGC TGGCATTGGG
    ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA GCAAGCCAAA GAGGATCAGC TGCGAGCATC
    ACTCCAGCTC CTGTGACTCC AGCAAAGGGC CTCCTCCAGG TTGCACCTAC ATCATCGAGC
    ATAGCTAAAC AGATGGAAAC TTCTAAAACG AACGAGAAGG CAAAGGCTAC AACTTCATCT
    ACGCACACAG ATAATGTAAT GCAGCTATCG ACAGCAGATG GGGTGATAAA TAACCTTCAA
    AGGGATGACT CTCTAGACTC CGGTTCCTAT GGAAAACAGG CTGGAACTCA TGATAGCGGT
    ACACCAGCCA AAACTATTGG CAGGTTTTCA GTAAGCAGCA CGCAAGATGA ATTAACTTTA
    GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG CCCCCGGACG TCTATTTGGA TGAGCTCCCT
    TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA CGGGTGCAGA CGGCCTCGTC TGTTGATGTC
    CTTTGTGACC AGGGCTCGAG TGATTCTGCC GAGGAGCCCT TCCATCGACA GGCTGCTGCG
    GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA GCCTCTGACT TGATTAAAAA AGCAGCAGCT
    TTTCTGCAAA GGACTGGAAA AGCAGGCTCC CAAGGCCCCG ATTCACCAAA CGGGCAGGGA
    ACAAAGATTC CTACCATCAA CATAACATCC TTCCACTCGC AGTCATCATA CATGAGCAGT
    GACAATGACT CTGAATTTGA AGATGCAGAC ATGAAGAAAG AACTGCAGAA CTTGAGAGAG
    AAGCATATGA AAGAAATTGC TGAACTTCAG ACTCAGCAGA AGAATGAGAT AGAGGCGCTG
    TACATTCGGT TAGGGAAGCC CCTCCCTCCC AACATGGGGT TCCTTCACTC AGCCCCGCCA
    AGCAGCCGTC GACGAAGAAC AAGCAAAAAT AAATTGAAAG GTGGAAAACT ATTAAACCCT
    CTGGTTCAAC AGCTCAGGAG TGGAACATCA AGCACAAGTA ATGTTTCAGA CTCTAAAGAC
    TCACCCCACA AAGAAACAAC TAAAGCTCAG CCTGGATCTC CCGAAACAAC AGGAGTAACT
    TCCTCTGTGT CAGCCACATT TGGGAAGTCT GTTCAGACAC AGCAGCCATG CTCTGTCAAA
    GCCTCTTTGT CTTCTGACAT CTGCTCCGGC TTAGGCCCCG AAGGAGGCGA TGTGAATGCA
    AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC CAAACGTCTG AGAGAGTGAC CTATAAATCT
    AGTAGCAAAC CTCGTACCAG ATTCCTCAGT GGACCTGTGT CTTTGTCCAT CTGGTCCACC
    TTGAAACGAC TATGTCTAGG CAAAGATCAC AGTAGTAGAT CCTCTACAAA CAGTCTTGTA
    ACGTGTCCTG AGCCCCTCCC GCAATCAACT CTGCAGGTTC AGGCCAACAA CAGTAACAAC
    AAGAAGGGAA CGTTCACCGA TGATCTTCAC AAGCTGGTTG ACCAGTGGAC AAGCAAAACT
    GTTGGAGCTG CACAGGCAAA ACCTTCTTTA AACCAACTGA AGCAGAACCA AAAAAGGCAG
    GACATGGAGC CAAAGGCAAA TCCCATGCAA ACACAGAATG AGACATGTGG AGTTAATTCA
    GTAAGAACAG GATTGGGGAA AAGGTGCATT GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG
    CTAACTCCTG GAGTTTCACC ATCCATGCCA GTGCCTATGC CAGTTTGCTC CATAGGCAGC
    ACGGACACCT CTCTCCTGTT TGCGGGTTGC TTTTTGCCTC TTTTACTGTG CTTAGAGTCT
    GCATCTGCAC CTCTCAGTCT AAAGGCACAA GGTCACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010411.1
    CDS203..6904
    Translation

    Target ORF information:

    RefSeq Version XM_025154643.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC AACCCATATC ACAGCAAACT
    TCTCTGCAGC AGGTGCTTGC CAGCCAGCCG GTTTGCCCCA TCCAGCCTGC CCCCCATCTC
    CTGCCACAGT ATCAGCCCCA GACCTCTCAG GTGGCAGCCA GTAGTACACC ACTGAAACCC
    CTTCAGATTT CAACTGTGCC ACAGCTTCCT CCAGTTGCCC CTTCCCAGAT TCCTCAGTTT
    CCCGTCATTC CTGCAATTAC TCCTCTGGCA GGACTTGACA ACCTTCCTCC AAACCTCTCT
    GATATACCTG CTGCAAACGT GCCCCCCATA CCTGCTCCTT CTCAGTATTT TGCTCCAGCT
    GTGATTTTAC CGAGCCTCCC CATGAACCCC ACTTTGCCCA TGGCACCAAA CTCCCCTGCC
    CTCCCCATGC AAGCAGTGAA CCTTCCTCAT ACAACTGTGG CTTCTCTGGT CTTGCCCTGC
    CAAACAATAG TGCCAAATAT GTCGGCTGCT ACAATACCGT TGCTTGCTGT GGCTCCGCCG
    GGAGTGCCAG CGCTGCCGCC GCACCACGCG GTGTCCCAGC TGCCCCAGCA GCAGATGTAC
    CCGACCACCT TCCAGCAGAT AATCCAGAGC GAAGCGCCCT CTCCCCATCA CACGCAGAGC
    ACGCAAGCCG TTTCCCAGCC GCAGCAGCCT CCACCTCCTC AGTCACTTCA GCCAGGCGTA
    ATTCATCCAT CAGAACAGCC GTTGTCCGCC CCTGGGGCTG GTAACCAGCA GGTTACTGGA
    CATACTCAGT ATGGTTTGGA AACTGGAGCC CAGGTGCCGG TACTCCCAGT GCAGCCTTCG
    CTAGTCATGT CACCGCCTTT GCAGTTGCAA CCTGAACTAT TGCCTCCCCG CGTCGCTCCA
    GAAAGTATTT CACAGATTCA TGGCAGCCTT GCTACAGCTA GTCCACCAGT GCCCATAGAT
    CACTGTCTGC CTCTCCAGCC TTCGGGTGTT CTGCAGCTTC AGCCAGACCT TTCCCAGCCT
    CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA GCCGTGCAGG CAGTGGCAGA GACATCGCAA
    GAGGAGCAGG CAATACAGGA CAAACTTCAA ACTCTGTCAC AGAGCTGTGA AAGCTACATG
    AGTCCAGATG TCGCTTCAGG TAAAGAGATG AGTGACAGCT TTGAAGGAGC AATAGGAAGT
    GGAAAACAAG AAGGAAAGTC TTCAAAGAAA CATAAGAAAT CTACACGTGC TCGTTCACGC
    CAGGAGAAAA CCAACAGACC CAAACTGACT ATATTAAATG TTTGTAACAC AGGGGATAAG
    ATGGTGGAGT GCCAGCTTGA AACACACAAT CACAAAATGG TGACTTTTAA GTTTGATTTG
    GATGGTGATG CGCCTGAGGA AATTGCTACT TACATGGTAG AGAATGAATT CATATTGCAG
    AGTGAGAAAG AGACGTTTAT TGAACAAATG AAAGATATTA TCGATAAAGC AGAAGATATG
    CTCAGTGAAG ATACAGAAGG AGAACGGAGT TCTGATCAGG GAACAAGTCC CCAGCAGGAT
    ACTGATGGAG CAGAAATGAG TGAGGAGAAG CGGCCATCTC AAGTGAAGAC ACCCGTGTAT
    CAACAAAATG TTTTGCATAC CGGAAAAAGG TGGTTTATTA TATGCCCTGT GGTGGAAAAC
    CCGACTACTG ATGCACCAGA ATTTTCTCCA CCAGTTCCTC AGAGTGCACA ACAGTCTGAA
    GGTCCAGACC TGGAGCAGTC GGATGATCAG CAAGCAAACT CTGCGGTACC GGATACGCCT
    GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA CAAGCAAGCG TCTGTGACAG CCCCATTGGG
    GCCTCTCCAT CTTTGGCACA AATTCCTGCT GCAGCATCTT CAGCTGGCTT GCCAAGCCAG
    GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA CCTGCTCCAA ACATCCTGGA GCAGGCAGCC
    GCTAACGGAG GCCAGCCGGA ATGTTCTCTA CTGAAAGCAG CATTGCCCGC CCCACCTGGC
    ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG GAGCCCGCTG ATGCTCCACT GGCTGCTCAG
    CCTACTCAGC ATGCGCAGCA GCAGGCAGAC GAGGCCATGT GTATCCCTGA TGTGGAGATA
    GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA GTCCCACCAG CTGCTGCCAA GGCACCGGAG
    CTCTGCCCGC TCCCTGTGCT CTCAGATGCT GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG
    ATCCCTCACC TGCCAGAGGC CAGCCAGCCC GGCGTGGTGC AGCACTCCTT TGTGAACAAA
    GTGTTTCCTC CATCTGTTGC ATCTGAGTCT CTCAATCTGG CAGGATCTCA ACTTCAGAGC
    CCCACTCAGG TGTCCATGGC CGCATCCCAG CAGCACGCGA TGCTGTCCCA GACACAGCCT
    GTCACCTGCG CCAGCGTGGG GATCAGCCTG CTGCAGCAGC AGCAACAGCA GCAGCCGTGC
    ACCGTGGAGT CTGATGGAGA GGGGCCACCT CGGGTGGACT TTGTTGACAA CACAATAAAA
    AGCCTCGATG AGAAACTGCG CAACTTACTT TACCAGGAAT ATGTCCCAAC TTCTTCTGCA
    TCAGCAGGAA CTCCAGACGC TTTTGTGCCA TTGGAACAGG CAGACAGTGA ATTTAACTTG
    CCACCACTTA CCGAAGATCA AGTTCCCAAG TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT
    AACACCACGG ATAGCTGCCA GGAGGTGAAA GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG
    CCACCTGAGA TCGCACCTTA TCCAGCATTG CTTGAAAAGT CTGAGGTCGC TGGCATTGGG
    ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA GCAAGCCAAA GAGGATCAGC TGCGAGCATC
    ACTCCAGCTC CTGTGACTCC AGCAAAGGGC CTCCTCCAGG TTGCACCTAC ATCATCGAGC
    ATAGCTAAAC AGATGGAAAC TTCTAAAACG AACGAGAAGG CAAAGGCTAC AACTTCATCT
    ACGCACACAG ATAATGTAAT GCAGCTATCG ACAGCAGATG GGGTGATAAA TAACCTTCAA
    AGGGATGACT CTCTAGACTC CGGTTCCTAT GGAAAACAGG CTGGAACTCA TGATAGCGGT
    ACACCAGCCA AAACTATTGG CAGGTTTTCA GTAAGCAGCA CGCAAGATGA ATTAACTTTA
    GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG CCCCCGGACG TCTATTTGGA TGAGCTCCCT
    TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA CGGGTGCAGA CGGCCTCGTC TGTTGATGTC
    CTTTGTGACC AGGGCTCGAG TGATTCTGCC GAGGAGCCCT TCCATCGACA GGCTGCTGCG
    GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA GCCTCTGACT TGATTAAAAA AGCAGCAGCT
    TTTCTGCAAA GGACTGGAAA AGCAGGCTCC CAAGGCCCCG ATTCACCAAA CGGGCAGGGA
    ACAAAGATTC CTACCATCAA CATAACATCC TTCCACTCGC AGTCATCATA CATGAGCAGT
    GACAATGACT CTGAATTTGA AGATGCAGAC ATGAAGAAAG AACTGCAGAA CTTGAGAGAG
    AAGCATATGA AAGAAATTGC TGAACTTCAG ACTCAGCAGA AGAATGAGAT AGAGGCGCTG
    TACATTCGGT TAGGGAAGCC CCTCCCTCCC AACATGGGGT TCCTTCACTC AGCCCCGCCA
    AGCAGCCGTC GACGAAGAAC AAGCAAAAAT AAATTGAAAG GTGGAAAACT ATTAAACCCT
    CTGGTTCAAC AGCTCAGGAG TGGAACATCA AGCACAAGTA ATGTTTCAGA CTCTAAAGAC
    TCACCCCACA AAGAAACAAC TAAAGCTCAG CCTGGATCTC CCGAAACAAC AGGAGTAACT
    TCCTCTGTGT CAGCCACATT TGGGAAGTCT GTTCAGACAC AGCAGCCATG CTCTGTCAAA
    GCCTCTTTGT CTTCTGACAT CTGCTCCGGC TTAGGCCCCG AAGGAGGCGA TGTGAATGCA
    AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC CAAACGTCTG AGAGAGTGAC CTATAAATCT
    AGTAGCAAAC CTCGTACCAG ATTCCTCAGT GGACCTGTGT CTTTGTCCAT CTGGTCCACC
    TTGAAACGAC TATGTCTAGG CAAAGATCAC AGTAGTAGAT CCTCTACAAA CAGTCTTGTA
    ACGTGTCCTG AGCCCCTCCC GCAATCAACT CTGCAGGTTC AGGCCAACAA CAGTAACAAC
    AAGAAGGGAA CGTTCACCGA TGATCTTCAC AAGCTGGTTG ACCAGTGGAC AAGCAAAACT
    GTTGGAGCTG CACAGGCAAA ACCTTCTTTA AACCAACTGA AGCAGAACCA AAAAAGGCAG
    GACATGGAGC CAAAGGCAAA TCCCATGCAA ACACAGAATG AGACATGTGG AGTTAATTCA
    GTAAGAACAG GATTGGGGAA AAGGTGCATT GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG
    CTAACTCCTG GAGTTTCACC ATCCATGCCA GTGCCTATGC CAGTTTGCTC CATAGGCAGC
    ACGGACACCT CTCTCCTGTT TGCGGGTTGC TTTTTGCCTC TTTTACTGTG CTTAGAGTCT
    GCATCTGCAC CTCTCAGTCT AAAGGCACAA GGTCACCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144019
    Clone ID Related Accession (Same CDS sequence) XM_025154644.1
    Accession Version XM_025154644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGAGCAGGCA ATACAGGACA AACTTCAAAC TCTGTCACAG
    AGCTGTGAAA GCTACATGAG TCCAGATGTC GCTTCAGGTA AAGAGATGAG TGACAGCTTT
    GAAGGAGCAA TAGGAAGTGG AAAACAAGAA GGAAAGTCTT CAAAGAAACA TAAGAAATCT
    ACACGTGCTC GTTCACGCCA GGAGAAAACC AACAGACCCA AACTGACTAT ATTAAATGTT
    TGTAACACAG GGGATAAGAT GGTGGAGTGC CAGCTTGAAA CACACAATCA CAAAATGGTG
    ACTTTTAAGT TTGATTTGGA TGGTGATGCG CCTGAGGAAA TTGCTACTTA CATGGTAGAG
    AATGAATTCA TATTGCAGAG TGAGAAAGAG ACGTTTATTG AACAAATGAA AGATATTATC
    GATAAAGCAG AAGATATGCT CAGTGAAGAT ACAGAAGGAG AACGGAGTTC TGATCAGGGA
    ACAAGTCCCC AGCAGGATAC TGATGGAGCA GAAATGAGTG AGGAGAAGCG GCCATCTCAA
    GTGAAGACAC CCGTGTATCA ACAAAATGTT TTGCATACCG GAAAAAGGTG GTTTATTATA
    TGCCCTGTGG TGGAAAACCC GACTACTGAT GCACCAGAAT TTTCTCCACC AGTTCCTCAG
    AGTGCACAAC AGTCTGAAGG TCCAGACCTG GAGCAGTCGG ATGATCAGCA AGCAAACTCT
    GCGGTACCGG ATACGCCTGG TGTCTTAGCT TGTCAGCAGC TTCCTCTACA AGCAAGCGTC
    TGTGACAGCC CCATTGGGGC CTCTCCATCT TTGGCACAAA TTCCTGCTGC AGCATCTTCA
    GCTGGCTTGC CAAGCCAGGC AGAGCTCTCA GTTCCAGCAC CGCCAGGACC TGCTCCAAAC
    ATCCTGGAGC AGGCAGCCGC TAACGGAGGC CAGCCGGAAT GTTCTCTACT GAAAGCAGCA
    TTGCCCGCCC CACCTGGCAC TCCTGTCCCA GGTGCTGTCC CTCTGGAGGA GCCCGCTGAT
    GCTCCACTGG CTGCTCAGCC TACTCAGCAT GCGCAGCAGC AGGCAGACGA GGCCATGTGT
    ATCCCTGATG TGGAGATAGC GTGTTGCAGT GTCGGGCCAC CCAAAGCAGT CCCACCAGCT
    GCTGCCAAGG CACCGGAGCT CTGCCCGCTC CCTGTGCTCT CAGATGCTGT TGTTTTGGAG
    CGGCAGCCTG CGGCCCAGAT CCCTCACCTG CCAGAGGCCA GCCAGCCCGG CGTGGTGCAG
    CACTCCTTTG TGAACAAAGT GTTTCCTCCA TCTGTTGCAT CTGAGTCTCT CAATCTGGCA
    GGATCTCAAC TTCAGAGCCC CACTCAGGTG TCCATGGCCG CATCCCAGCA GCACGCGATG
    CTGTCCCAGA CACAGCCTGT CACCTGCGCC AGCGTGGGGA TCAGCCTGCT GCAGCAGCAG
    CAACAGCAGC AGCCGTGCAC CGTGGAGTCT GATGGAGAGG GGCCACCTCG GGTGGACTTT
    GTTGACAACA CAATAAAAAG CCTCGATGAG AAACTGCGCA ACTTACTTTA CCAGGAATAT
    GTCCCAACTT CTTCTGCATC AGCAGGAACT CCAGACGCTT TTGTGCCATT GGAACAGGCA
    GACAGTGAAT TTAACTTGCC ACCACTTACC GAAGATCAAG TTCCCAAGTT GGTGTTGGAT
    TTGAGAGAAC CAGGCCATAA CACCACGGAT AGCTGCCAGG AGGTGAAAGC TGCCGAGGCC
    CAGTTTGTGC CGGCTGTGCC ACCTGAGATC GCACCTTATC CAGCATTGCT TGAAAAGTCT
    GAGGTCGCTG GCATTGGGAC TGAGTCTTTG GAAGCAAGAG GTGGGTCAGC AAGCCAAAGA
    GGATCAGCTG CGAGCATCAC TCCAGCTCCT GTGACTCCAG CAAAGGGCCT CCTCCAGGTT
    GCACCTACAT CATCGAGCAT AGCTAAACAG ATGGAAACTT CTAAAACGAA CGAGAAGGCA
    AAGGCTACAA CTTCATCTAC GCACACAGAT AATGTAATGC AGCTATCGAC AGCAGATGGG
    GTGATAAATA ACCTTCAAAG GGATGACTCT CTAGACTCCG GTTCCTATGG AAAACAGGCT
    GGAACTCATG ATAGCGGTAC ACCAGCCAAA ACTATTGGCA GGTTTTCAGT AAGCAGCACG
    CAAGATGAAT TAACTTTAGC AGCGCCGCAT TGCTTAAGAT TCTCTGCGCC CCCGGACGTC
    TATTTGGATG AGCTCCCTTC CAGCCCCGAC CAGAAGACAG CTGTCCGACG GGTGCAGACG
    GCCTCGTCTG TTGATGTCCT TTGTGACCAG GGCTCGAGTG ATTCTGCCGA GGAGCCCTTC
    CATCGACAGG CTGCTGCGGC TCAGCCATCC CCTCCGAGCA GTAGTGCAGC CTCTGACTTG
    ATTAAAAAAG CAGCAGCTTT TCTGCAAAGG ACTGGAAAAG CAGGCTCCCA AGGCCCCGAT
    TCACCAAACG GGCAGGGAAC AAAGATTCCT ACCATCAACA TAACATCCTT CCACTCGCAG
    TCATCATACA TGAGCAGTGA CAATGACTCT GAATTTGAAG ATGCAGACAT GAAGAAAGAA
    CTGCAGAACT TGAGAGAGAA GCATATGAAA GAAATTGCTG AACTTCAGAC TCAGCAGAAG
    AATGAGATAG AGGCGCTGTA CATTCGGTTA GGGAAGCCCC TCCCTCCCAA CATGGGGTTC
    CTTCACTCAG CCCCGCCAAG CAGCCGTCGA CGAAGAACAA GCAAAAATAA ATTGAAAGGT
    GGAAAACTAT TAAACCCTCT GGTTCAACAG CTCAGGAGTG GAACATCAAG CACAAGTAAT
    GTTTCAGACT CTAAAGACTC ACCCCACAAA GAAACAACTA AAGCTCAGCC TGGATCTCCC
    GAAACAACAG GAGTAACTTC CTCTGTGTCA GCCACATTTG GGAAGTCTGT TCAGACACAG
    CAGCCATGCT CTGTCAAAGC CTCTTTGTCT TCTGACATCT GCTCCGGCTT AGGCCCCGAA
    GGAGGCGATG TGAATGCAAG CTCTGGCCAA GGCTGGACGG TTTTCCACCA AACGTCTGAG
    AGAGTGACCT ATAAATCTAG TAGCAAACCT CGTACCAGAT TCCTCAGTGG ACCTGTGTCT
    TTGTCCATCT GGTCCACCTT GAAACGACTA TGTCTAGGCA AAGATCACAG TAGTAGATCC
    TCTACAAACA GTCTTGTAAC GTGTCCTGAG CCCCTCCCGC AATCAACTCT GCAGGTTCAG
    GCCAACAACA GTAACAACAA GAAGGGAACG TTCACCGATG ATCTTCACAA GCTGGTTGAC
    CAGTGGACAA GCAAAACTGT TGGAGCTGCA CAGGCAAAAC CTTCTTTAAA CCAACTGAAG
    CAGAACCAAA AAAGGCAGGA CATGGAGCCA AAGGCAAATC CCATGCAAAC ACAGAATGAG
    ACATGTGGAG TTAATTCAGT AAGAACAGGA TTGGGGAAAA GGTGCATTGT TCCAGTGTCT
    TGTCCCATGT CAGCTGCGCT AACTCCTGGA GTTTCACCAT CCATGCCAGT GCCTATGCCA
    GTTTGCTCCA TAGGCAGCAC GGACACCTCT CTCCTGTTTG CGGGTTGCTT TTTGCCTCTT
    TTACTGTGCT TAGAGTCTGC ATCTGCACCT CTCAGTCTAA AGGCACAAGG TCACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010412.1
    CDS202..6741
    Translation

    Target ORF information:

    RefSeq Version XM_025154644.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGAGCAGGCA ATACAGGACA AACTTCAAAC TCTGTCACAG
    AGCTGTGAAA GCTACATGAG TCCAGATGTC GCTTCAGGTA AAGAGATGAG TGACAGCTTT
    GAAGGAGCAA TAGGAAGTGG AAAACAAGAA GGAAAGTCTT CAAAGAAACA TAAGAAATCT
    ACACGTGCTC GTTCACGCCA GGAGAAAACC AACAGACCCA AACTGACTAT ATTAAATGTT
    TGTAACACAG GGGATAAGAT GGTGGAGTGC CAGCTTGAAA CACACAATCA CAAAATGGTG
    ACTTTTAAGT TTGATTTGGA TGGTGATGCG CCTGAGGAAA TTGCTACTTA CATGGTAGAG
    AATGAATTCA TATTGCAGAG TGAGAAAGAG ACGTTTATTG AACAAATGAA AGATATTATC
    GATAAAGCAG AAGATATGCT CAGTGAAGAT ACAGAAGGAG AACGGAGTTC TGATCAGGGA
    ACAAGTCCCC AGCAGGATAC TGATGGAGCA GAAATGAGTG AGGAGAAGCG GCCATCTCAA
    GTGAAGACAC CCGTGTATCA ACAAAATGTT TTGCATACCG GAAAAAGGTG GTTTATTATA
    TGCCCTGTGG TGGAAAACCC GACTACTGAT GCACCAGAAT TTTCTCCACC AGTTCCTCAG
    AGTGCACAAC AGTCTGAAGG TCCAGACCTG GAGCAGTCGG ATGATCAGCA AGCAAACTCT
    GCGGTACCGG ATACGCCTGG TGTCTTAGCT TGTCAGCAGC TTCCTCTACA AGCAAGCGTC
    TGTGACAGCC CCATTGGGGC CTCTCCATCT TTGGCACAAA TTCCTGCTGC AGCATCTTCA
    GCTGGCTTGC CAAGCCAGGC AGAGCTCTCA GTTCCAGCAC CGCCAGGACC TGCTCCAAAC
    ATCCTGGAGC AGGCAGCCGC TAACGGAGGC CAGCCGGAAT GTTCTCTACT GAAAGCAGCA
    TTGCCCGCCC CACCTGGCAC TCCTGTCCCA GGTGCTGTCC CTCTGGAGGA GCCCGCTGAT
    GCTCCACTGG CTGCTCAGCC TACTCAGCAT GCGCAGCAGC AGGCAGACGA GGCCATGTGT
    ATCCCTGATG TGGAGATAGC GTGTTGCAGT GTCGGGCCAC CCAAAGCAGT CCCACCAGCT
    GCTGCCAAGG CACCGGAGCT CTGCCCGCTC CCTGTGCTCT CAGATGCTGT TGTTTTGGAG
    CGGCAGCCTG CGGCCCAGAT CCCTCACCTG CCAGAGGCCA GCCAGCCCGG CGTGGTGCAG
    CACTCCTTTG TGAACAAAGT GTTTCCTCCA TCTGTTGCAT CTGAGTCTCT CAATCTGGCA
    GGATCTCAAC TTCAGAGCCC CACTCAGGTG TCCATGGCCG CATCCCAGCA GCACGCGATG
    CTGTCCCAGA CACAGCCTGT CACCTGCGCC AGCGTGGGGA TCAGCCTGCT GCAGCAGCAG
    CAACAGCAGC AGCCGTGCAC CGTGGAGTCT GATGGAGAGG GGCCACCTCG GGTGGACTTT
    GTTGACAACA CAATAAAAAG CCTCGATGAG AAACTGCGCA ACTTACTTTA CCAGGAATAT
    GTCCCAACTT CTTCTGCATC AGCAGGAACT CCAGACGCTT TTGTGCCATT GGAACAGGCA
    GACAGTGAAT TTAACTTGCC ACCACTTACC GAAGATCAAG TTCCCAAGTT GGTGTTGGAT
    TTGAGAGAAC CAGGCCATAA CACCACGGAT AGCTGCCAGG AGGTGAAAGC TGCCGAGGCC
    CAGTTTGTGC CGGCTGTGCC ACCTGAGATC GCACCTTATC CAGCATTGCT TGAAAAGTCT
    GAGGTCGCTG GCATTGGGAC TGAGTCTTTG GAAGCAAGAG GTGGGTCAGC AAGCCAAAGA
    GGATCAGCTG CGAGCATCAC TCCAGCTCCT GTGACTCCAG CAAAGGGCCT CCTCCAGGTT
    GCACCTACAT CATCGAGCAT AGCTAAACAG ATGGAAACTT CTAAAACGAA CGAGAAGGCA
    AAGGCTACAA CTTCATCTAC GCACACAGAT AATGTAATGC AGCTATCGAC AGCAGATGGG
    GTGATAAATA ACCTTCAAAG GGATGACTCT CTAGACTCCG GTTCCTATGG AAAACAGGCT
    GGAACTCATG ATAGCGGTAC ACCAGCCAAA ACTATTGGCA GGTTTTCAGT AAGCAGCACG
    CAAGATGAAT TAACTTTAGC AGCGCCGCAT TGCTTAAGAT TCTCTGCGCC CCCGGACGTC
    TATTTGGATG AGCTCCCTTC CAGCCCCGAC CAGAAGACAG CTGTCCGACG GGTGCAGACG
    GCCTCGTCTG TTGATGTCCT TTGTGACCAG GGCTCGAGTG ATTCTGCCGA GGAGCCCTTC
    CATCGACAGG CTGCTGCGGC TCAGCCATCC CCTCCGAGCA GTAGTGCAGC CTCTGACTTG
    ATTAAAAAAG CAGCAGCTTT TCTGCAAAGG ACTGGAAAAG CAGGCTCCCA AGGCCCCGAT
    TCACCAAACG GGCAGGGAAC AAAGATTCCT ACCATCAACA TAACATCCTT CCACTCGCAG
    TCATCATACA TGAGCAGTGA CAATGACTCT GAATTTGAAG ATGCAGACAT GAAGAAAGAA
    CTGCAGAACT TGAGAGAGAA GCATATGAAA GAAATTGCTG AACTTCAGAC TCAGCAGAAG
    AATGAGATAG AGGCGCTGTA CATTCGGTTA GGGAAGCCCC TCCCTCCCAA CATGGGGTTC
    CTTCACTCAG CCCCGCCAAG CAGCCGTCGA CGAAGAACAA GCAAAAATAA ATTGAAAGGT
    GGAAAACTAT TAAACCCTCT GGTTCAACAG CTCAGGAGTG GAACATCAAG CACAAGTAAT
    GTTTCAGACT CTAAAGACTC ACCCCACAAA GAAACAACTA AAGCTCAGCC TGGATCTCCC
    GAAACAACAG GAGTAACTTC CTCTGTGTCA GCCACATTTG GGAAGTCTGT TCAGACACAG
    CAGCCATGCT CTGTCAAAGC CTCTTTGTCT TCTGACATCT GCTCCGGCTT AGGCCCCGAA
    GGAGGCGATG TGAATGCAAG CTCTGGCCAA GGCTGGACGG TTTTCCACCA AACGTCTGAG
    AGAGTGACCT ATAAATCTAG TAGCAAACCT CGTACCAGAT TCCTCAGTGG ACCTGTGTCT
    TTGTCCATCT GGTCCACCTT GAAACGACTA TGTCTAGGCA AAGATCACAG TAGTAGATCC
    TCTACAAACA GTCTTGTAAC GTGTCCTGAG CCCCTCCCGC AATCAACTCT GCAGGTTCAG
    GCCAACAACA GTAACAACAA GAAGGGAACG TTCACCGATG ATCTTCACAA GCTGGTTGAC
    CAGTGGACAA GCAAAACTGT TGGAGCTGCA CAGGCAAAAC CTTCTTTAAA CCAACTGAAG
    CAGAACCAAA AAAGGCAGGA CATGGAGCCA AAGGCAAATC CCATGCAAAC ACAGAATGAG
    ACATGTGGAG TTAATTCAGT AAGAACAGGA TTGGGGAAAA GGTGCATTGT TCCAGTGTCT
    TGTCCCATGT CAGCTGCGCT AACTCCTGGA GTTTCACCAT CCATGCCAGT GCCTATGCCA
    GTTTGCTCCA TAGGCAGCAC GGACACCTCT CTCCTGTTTG CGGGTTGCTT TTTGCCTCTT
    TTACTGTGCT TAGAGTCTGC ATCTGCACCT CTCAGTCTAA AGGCACAAGG TCACCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa144020
    Clone ID Related Accession (Same CDS sequence) XM_025154645.1
    Accession Version XM_025154645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025010413.1
    CDS203..6697
    Translation

    Target ORF information:

    RefSeq Version XM_025154645.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025154645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41672
    Clone ID Related Accession (Same CDS sequence) XM_015293416.1
    Accession Version XM_015293416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGGTAC TACCCCTTCT GGCGCAGTAA CGCCCTGTGT GATGCCTCTT
    TGTCAGTACG GAGGAGTTTT CCCTACGCCG CTTTATGGAG TGCAGTGGTC GGGGCCGGCA
    GTGCGGCCGG GGCTGGTAGG AGGGCAGAGC ATTGCTCATT TCTCAGCTCT CAGCAAAGCT
    TGCTTGCAGA TGTACCCTGT GCCGCTGCAG CAGCCCGCAG CCGACCCGCC CGGCCCGCGT
    GTAAGAATGA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148902.1
    CDS183..7157
    Translation

    Target ORF information:

    RefSeq Version XM_015293416.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGGTAC TACCCCTTCT GGCGCAGTAA CGCCCTGTGT GATGCCTCTT
    TGTCAGTACG GAGGAGTTTT CCCTACGCCG CTTTATGGAG TGCAGTGGTC GGGGCCGGCA
    GTGCGGCCGG GGCTGGTAGG AGGGCAGAGC ATTGCTCATT TCTCAGCTCT CAGCAAAGCT
    TGCTTGCAGA TGTACCCTGT GCCGCTGCAG CAGCCCGCAG CCGACCCGCC CGGCCCGCGT
    GTAAGAATGA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41673
    Clone ID Related Accession (Same CDS sequence) XM_015293417.1
    Accession Version XM_015293417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGTTACTGGA CATACTCAGT ATGGTTTGGA AACTGGAGCC
    CAGGTGCCGG TACTCCCAGT GCAGCCTTCG CTAGTCATGT CACCGCCTTT GCAGTTGCAA
    CCTGAACTAT TGCCTCCCCG CGTCGCTCCA GAAAGTATTT CACAGATTCA TGGCAGCCTT
    GCTACAGCTA GTCCACCAGT GCCCATAGAT CACTGTCTGC CTCTCCAGCC TTCGGGTGTT
    CTGCAGCTTC AGCCAGACCT TTCCCAGCCT CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA
    GCCGTGCAGG CAGTGGCAGA GACATCGCAA GAGGAGCAGG CAATACAGGA CAAACTTCAA
    ACTCTGTCAC AGAGCTGTGA AAGCTACATG AGTCCAGATG TCGCTTCAGG TAAAGAGATG
    AGTGACAGCT TTGAAGGAGC AATAGGAAGT GGAAAACAAG AAGGAAAGTC TTCAAAGAAA
    CATAAGAAAT CTACACGTGC TCGTTCACGC CAGGAGAAAA CCAACAGACC CAAACTGACT
    ATATTAAATG TTTGTAACAC AGGGGATAAG ATGGTGGAGT GCCAGCTTGA AACACACAAT
    CACAAAATGG TGACTTTTAA GTTTGATTTG GATGGTGATG CGCCTGAGGA AATTGCTACT
    TACATGGTAG AGAATGAATT CATATTGCAG AGTGAGAAAG AGACGTTTAT TGAACAAATG
    AAAGATATTA TCGATAAAGC AGAAGATATG CTCAGTGAAG ATACAGAAGG AGAACGGAGT
    TCTGATCAGG GAACAAGTCC CCAGCAGGAT ACTGATGGAG CAGAAATGAG TGAGGAGAAG
    CGGCCATCTC AAGTGAAGAC ACCCGTGTAT CAACAAAATG TTTTGCATAC CGGAAAAAGG
    TGGTTTATTA TATGCCCTGT GGTGGAAAAC CCGACTACTG ATGCACCAGA ATTTTCTCCA
    CCAGTTCCTC AGAGTGCACA ACAGTCTGAA GGTCCAGACC TGGAGCAGTC GGATGATCAG
    CAAGCAAACT CTGCGGTACC GGATACGCCT GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA
    CAAGCAAGCG TCTGTGACAG CCCCATTGGG GCCTCTCCAT CTTTGGCACA AATTCCTGCT
    GCAGCATCTT CAGCTGGCTT GCCAAGCCAG GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA
    CCTGCTCCAA ACATCCTGGA GCAGGCAGCC GCTAACGGAG GCCAGCCGGA ATGTTCTCTA
    CTGAAAGCAG CATTGCCCGC CCCACCTGGC ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG
    GAGCCCGCTG ATGCTCCACT GGCTGCTCAG CCTACTCAGC ATGCGCAGCA GCAGGCAGAC
    GAGGCCATGT GTATCCCTGA TGTGGAGATA GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA
    GTCCCACCAG CTGCTGCCAA GGCACCGGAG CTCTGCCCGC TCCCTGTGCT CTCAGATGCT
    GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG ATCCCTCACC TGCCAGAGGC CAGCCAGCCC
    GGCGTGGTGC AGCACTCCTT TGTGAACAAA GTGTTTCCTC CATCTGTTGC ATCTGAGTCT
    CTCAATCTGG CAGGATCTCA ACTTCAGAGC CCCACTCAGG TGTCCATGGC CGCATCCCAG
    CAGCACGCGA TGCTGTCCCA GACACAGCCT GTCACCTGCG CCAGCGTGGG GATCAGCCTG
    CTGCAGCAGC AGCAACAGCA GCAGCCGTGC ACCGTGGAGT CTGATGGAGA GGGGCCACCT
    CGGGTGGACT TTGTTGACAA CACAATAAAA AGCCTCGATG AGAAACTGCG CAACTTACTT
    TACCAGGAAT ATGTCCCAAC TTCTTCTGCA TCAGCAGGAA CTCCAGACGC TTTTGTGCCA
    TTGGAACAGG CAGACAGTGA ATTTAACTTG CCACCACTTA CCGAAGATCA AGTTCCCAAG
    TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT AACACCACGG ATAGCTGCCA GGAGGTGAAA
    GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG CCACCTGAGA TCGCACCTTA TCCAGCATTG
    CTTGAAAAGT CTGAGGTCGC TGGCATTGGG ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA
    GCAAGCCAAA GAGGATCAGC TGCGAGCATC ACTCCAGCTC CTGTGACTCC AGCAAAGGGC
    CTCCTCCAGG TTGCACCTAC ATCATCGAGC ATAGCTAAAC AGATGGAAAC TTCTAAAACG
    AACGAGAAGG CAAAGGCTAC AACTTCATCT ACGCACACAG ATAATGTAAT GCAGCTATCG
    ACAGCAGATG GGGTGATAAA TAACCTTCAA AGGGATGACT CTCTAGACTC CGGTTCCTAT
    GGAAAACAGG CTGGAACTCA TGATAGCGGT ACACCAGCCA AAACTATTGG CAGGTTTTCA
    GTAAGCAGCA CGCAAGATGA ATTAACTTTA GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG
    CCCCCGGACG TCTATTTGGA TGAGCTCCCT TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA
    CGGGTGCAGA CGGCCTCGTC TGTTGATGTC CTTTGTGACC AGGGCTCGAG TGATTCTGCC
    GAGGAGCCCT TCCATCGACA GGCTGCTGCG GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA
    GCCTCTGACT TGATTAAAAA AGCAGCAGCT TTTCTGCAAA GGACTGGAAA AGCAGGCTCC
    CAAGGCCCCG ATTCACCAAA CGGGCAGGGA ACAAAGATTC CTACCATCAA CATAACATCC
    TTCCACTCGC AGTCATCATA CATGAGCAGT GACAATGACT CTGAATTTGA AGATGCAGAC
    ATGAAGAAAG AACTGCAGAA CTTGAGAGAG AAGCATATGA AAGAAATTGC TGAACTTCAG
    ACTCAGCAGA AGAATGAGAT AGAGGCGCTG TACATTCGGT TAGGGAAGCC CCTCCCTCCC
    AACATGGGGT TCCTTCACTC AGCCCCGCCA AGCAGCCGTC GACGAAGAAC AAGCAAAAAT
    AAATTGAAAG GTGGAAAACT ATTAAACCCT CTGGTTCAAC AGCTCAGGAG TGGAACATCA
    AGCACAAGTA ATGTTTCAGA CTCTAAAGAC TCACCCCACA AAGAAACAAC TAAAGCTCAG
    CCTGGATCTC CCGAAACAAC AGGAGTAACT TCCTCTGTGT CAGCCACATT TGGGAAGTCT
    GTTCAGACAC AGCAGCCATG CTCTGTCAAA GCCTCTTTGT CTTCTGACAT CTGCTCCGGC
    TTAGGCCCCG AAGGAGGCGA TGTGAATGCA AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC
    CAAACGTCTG AGAGAGTGAC CTATAAATCT AGTAGCAAAC CTCGTACCAG ATTCCTCAGT
    GGACCTGTGT CTTTGTCCAT CTGGTCCACC TTGAAACGAC TATGTCTAGG CAAAGATCAC
    AGTAGTAGAT CCTCTACAAA CAGTCTTGTA ACGTGTCCTG AGCCCCTCCC GCAATCAACT
    CTGCAGGTTC AGGCCAACAA CAGTAACAAC AAGAAGGGAA CGTTCACCGA TGATCTTCAC
    AAGCTGGTTG ACCAGTGGAC AAGCAAAACT GTTGGAGCTG CACAGGCAAA ACCTTCTTTA
    AACCAACTGA AGCAGAACCA AAAAAGGCAG GACATGGAGC CAAAGGCAAA TCCCATGCAA
    ACACAGAATG AGACATGTGG AGTTAATTCA GTAAGAACAG GATTGGGGAA AAGGTGCATT
    GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG CTAACTCCTG GAGTTTCACC ATCCATGCCA
    GTGCCTATGC CAGGTACTAC CCCTTCTGGC GCAGTAACGC CCTGTGTGAT GCCTCTTTGT
    CAGTACGGAG GAGTTTTCCC TACGCCGCTT TATGGAGTGC AGTGGTCGGG GCCGGCAGTG
    CGGCCGGGGC TGGTAGGAGG GCAGAGCATT GCTCATTTCT CAGCTCTCAG CAAAGCTTGC
    TTGCAGATGT ACCCTGTGCC GCTGCAGCAG CCCGCAGCCG ACCCGCCCGG CCCGCGTGTA
    AGAATGACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148903.1
    CDS184..7155
    Translation

    Target ORF information:

    RefSeq Version XM_015293417.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGTTACTGGA CATACTCAGT ATGGTTTGGA AACTGGAGCC
    CAGGTGCCGG TACTCCCAGT GCAGCCTTCG CTAGTCATGT CACCGCCTTT GCAGTTGCAA
    CCTGAACTAT TGCCTCCCCG CGTCGCTCCA GAAAGTATTT CACAGATTCA TGGCAGCCTT
    GCTACAGCTA GTCCACCAGT GCCCATAGAT CACTGTCTGC CTCTCCAGCC TTCGGGTGTT
    CTGCAGCTTC AGCCAGACCT TTCCCAGCCT CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA
    GCCGTGCAGG CAGTGGCAGA GACATCGCAA GAGGAGCAGG CAATACAGGA CAAACTTCAA
    ACTCTGTCAC AGAGCTGTGA AAGCTACATG AGTCCAGATG TCGCTTCAGG TAAAGAGATG
    AGTGACAGCT TTGAAGGAGC AATAGGAAGT GGAAAACAAG AAGGAAAGTC TTCAAAGAAA
    CATAAGAAAT CTACACGTGC TCGTTCACGC CAGGAGAAAA CCAACAGACC CAAACTGACT
    ATATTAAATG TTTGTAACAC AGGGGATAAG ATGGTGGAGT GCCAGCTTGA AACACACAAT
    CACAAAATGG TGACTTTTAA GTTTGATTTG GATGGTGATG CGCCTGAGGA AATTGCTACT
    TACATGGTAG AGAATGAATT CATATTGCAG AGTGAGAAAG AGACGTTTAT TGAACAAATG
    AAAGATATTA TCGATAAAGC AGAAGATATG CTCAGTGAAG ATACAGAAGG AGAACGGAGT
    TCTGATCAGG GAACAAGTCC CCAGCAGGAT ACTGATGGAG CAGAAATGAG TGAGGAGAAG
    CGGCCATCTC AAGTGAAGAC ACCCGTGTAT CAACAAAATG TTTTGCATAC CGGAAAAAGG
    TGGTTTATTA TATGCCCTGT GGTGGAAAAC CCGACTACTG ATGCACCAGA ATTTTCTCCA
    CCAGTTCCTC AGAGTGCACA ACAGTCTGAA GGTCCAGACC TGGAGCAGTC GGATGATCAG
    CAAGCAAACT CTGCGGTACC GGATACGCCT GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA
    CAAGCAAGCG TCTGTGACAG CCCCATTGGG GCCTCTCCAT CTTTGGCACA AATTCCTGCT
    GCAGCATCTT CAGCTGGCTT GCCAAGCCAG GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA
    CCTGCTCCAA ACATCCTGGA GCAGGCAGCC GCTAACGGAG GCCAGCCGGA ATGTTCTCTA
    CTGAAAGCAG CATTGCCCGC CCCACCTGGC ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG
    GAGCCCGCTG ATGCTCCACT GGCTGCTCAG CCTACTCAGC ATGCGCAGCA GCAGGCAGAC
    GAGGCCATGT GTATCCCTGA TGTGGAGATA GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA
    GTCCCACCAG CTGCTGCCAA GGCACCGGAG CTCTGCCCGC TCCCTGTGCT CTCAGATGCT
    GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG ATCCCTCACC TGCCAGAGGC CAGCCAGCCC
    GGCGTGGTGC AGCACTCCTT TGTGAACAAA GTGTTTCCTC CATCTGTTGC ATCTGAGTCT
    CTCAATCTGG CAGGATCTCA ACTTCAGAGC CCCACTCAGG TGTCCATGGC CGCATCCCAG
    CAGCACGCGA TGCTGTCCCA GACACAGCCT GTCACCTGCG CCAGCGTGGG GATCAGCCTG
    CTGCAGCAGC AGCAACAGCA GCAGCCGTGC ACCGTGGAGT CTGATGGAGA GGGGCCACCT
    CGGGTGGACT TTGTTGACAA CACAATAAAA AGCCTCGATG AGAAACTGCG CAACTTACTT
    TACCAGGAAT ATGTCCCAAC TTCTTCTGCA TCAGCAGGAA CTCCAGACGC TTTTGTGCCA
    TTGGAACAGG CAGACAGTGA ATTTAACTTG CCACCACTTA CCGAAGATCA AGTTCCCAAG
    TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT AACACCACGG ATAGCTGCCA GGAGGTGAAA
    GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG CCACCTGAGA TCGCACCTTA TCCAGCATTG
    CTTGAAAAGT CTGAGGTCGC TGGCATTGGG ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA
    GCAAGCCAAA GAGGATCAGC TGCGAGCATC ACTCCAGCTC CTGTGACTCC AGCAAAGGGC
    CTCCTCCAGG TTGCACCTAC ATCATCGAGC ATAGCTAAAC AGATGGAAAC TTCTAAAACG
    AACGAGAAGG CAAAGGCTAC AACTTCATCT ACGCACACAG ATAATGTAAT GCAGCTATCG
    ACAGCAGATG GGGTGATAAA TAACCTTCAA AGGGATGACT CTCTAGACTC CGGTTCCTAT
    GGAAAACAGG CTGGAACTCA TGATAGCGGT ACACCAGCCA AAACTATTGG CAGGTTTTCA
    GTAAGCAGCA CGCAAGATGA ATTAACTTTA GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG
    CCCCCGGACG TCTATTTGGA TGAGCTCCCT TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA
    CGGGTGCAGA CGGCCTCGTC TGTTGATGTC CTTTGTGACC AGGGCTCGAG TGATTCTGCC
    GAGGAGCCCT TCCATCGACA GGCTGCTGCG GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA
    GCCTCTGACT TGATTAAAAA AGCAGCAGCT TTTCTGCAAA GGACTGGAAA AGCAGGCTCC
    CAAGGCCCCG ATTCACCAAA CGGGCAGGGA ACAAAGATTC CTACCATCAA CATAACATCC
    TTCCACTCGC AGTCATCATA CATGAGCAGT GACAATGACT CTGAATTTGA AGATGCAGAC
    ATGAAGAAAG AACTGCAGAA CTTGAGAGAG AAGCATATGA AAGAAATTGC TGAACTTCAG
    ACTCAGCAGA AGAATGAGAT AGAGGCGCTG TACATTCGGT TAGGGAAGCC CCTCCCTCCC
    AACATGGGGT TCCTTCACTC AGCCCCGCCA AGCAGCCGTC GACGAAGAAC AAGCAAAAAT
    AAATTGAAAG GTGGAAAACT ATTAAACCCT CTGGTTCAAC AGCTCAGGAG TGGAACATCA
    AGCACAAGTA ATGTTTCAGA CTCTAAAGAC TCACCCCACA AAGAAACAAC TAAAGCTCAG
    CCTGGATCTC CCGAAACAAC AGGAGTAACT TCCTCTGTGT CAGCCACATT TGGGAAGTCT
    GTTCAGACAC AGCAGCCATG CTCTGTCAAA GCCTCTTTGT CTTCTGACAT CTGCTCCGGC
    TTAGGCCCCG AAGGAGGCGA TGTGAATGCA AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC
    CAAACGTCTG AGAGAGTGAC CTATAAATCT AGTAGCAAAC CTCGTACCAG ATTCCTCAGT
    GGACCTGTGT CTTTGTCCAT CTGGTCCACC TTGAAACGAC TATGTCTAGG CAAAGATCAC
    AGTAGTAGAT CCTCTACAAA CAGTCTTGTA ACGTGTCCTG AGCCCCTCCC GCAATCAACT
    CTGCAGGTTC AGGCCAACAA CAGTAACAAC AAGAAGGGAA CGTTCACCGA TGATCTTCAC
    AAGCTGGTTG ACCAGTGGAC AAGCAAAACT GTTGGAGCTG CACAGGCAAA ACCTTCTTTA
    AACCAACTGA AGCAGAACCA AAAAAGGCAG GACATGGAGC CAAAGGCAAA TCCCATGCAA
    ACACAGAATG AGACATGTGG AGTTAATTCA GTAAGAACAG GATTGGGGAA AAGGTGCATT
    GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG CTAACTCCTG GAGTTTCACC ATCCATGCCA
    GTGCCTATGC CAGGTACTAC CCCTTCTGGC GCAGTAACGC CCTGTGTGAT GCCTCTTTGT
    CAGTACGGAG GAGTTTTCCC TACGCCGCTT TATGGAGTGC AGTGGTCGGG GCCGGCAGTG
    CGGCCGGGGC TGGTAGGAGG GCAGAGCATT GCTCATTTCT CAGCTCTCAG CAAAGCTTGC
    TTGCAGATGT ACCCTGTGCC GCTGCAGCAG CCCGCAGCCG ACCCGCCCGG CCCGCGTGTA
    AGAATGACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41674
    Clone ID Related Accession (Same CDS sequence) XM_015293420.1
    Accession Version XM_015293420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6954bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGATCA
    GCTGCGAGCA TCACTCCAGC TCCTGTGACT CCAGCAAAGG GCCTCCTCCA GGTTGCACCT
    ACATCATCGA GCATAGCTAA ACAGATGGAA ACTTCTAAAA CGAACGAGAA GGCAAAGGCT
    ACAACTTCAT CTACGCACAC AGATAATGTA ATGCAGCTAT CGACAGCAGA TGGGGTGATA
    AATAACCTTC AAAGGGATGA CTCTCTAGAC TCCGGTTCCT ATGGAAAACA GGCTGGAACT
    CATGATAGCG GTACACCAGC CAAAACTATT GGCAGGTTTT CAGTAAGCAG CACGCAAGAT
    GAATTAACTT TAGCAGCGCC GCATTGCTTA AGATTCTCTG CGCCCCCGGA CGTCTATTTG
    GATGAGCTCC CTTCCAGCCC CGACCAGAAG ACAGCTGTCC GACGGGTGCA GACGGCCTCG
    TCTGTTGATG TCCTTTGTGA CCAGGGCTCG AGTGATTCTG CCGAGGAGCC CTTCCATCGA
    CAGGCTGCTG CGGCTCAGCC ATCCCCTCCG AGCAGTAGTG CAGCCTCTGA CTTGATTAAA
    AAAGCAGCAG CTTTTCTGCA AAGGACTGGA AAAGCAGGCT CCCAAGGCCC CGATTCACCA
    AACGGGCAGG GAACAAAGAT TCCTACCATC AACATAACAT CCTTCCACTC GCAGTCATCA
    TACATGAGCA GTGACAATGA CTCTGAATTT GAAGATGCAG ACATGAAGAA AGAACTGCAG
    AACTTGAGAG AGAAGCATAT GAAAGAAATT GCTGAACTTC AGACTCAGCA GAAGAATGAG
    ATAGAGGCGC TGTACATTCG GTTAGGGAAG CCCCTCCCTC CCAACATGGG GTTCCTTCAC
    TCAGCCCCGC CAAGCAGCCG TCGACGAAGA ACAAGCAAAA ATAAATTGAA AGGTGGAAAA
    CTATTAAACC CTCTGGTTCA ACAGCTCAGG AGTGGAACAT CAAGCACAAG TAATGTTTCA
    GACTCTAAAG ACTCACCCCA CAAAGAAACA ACTAAAGCTC AGCCTGGATC TCCCGAAACA
    ACAGGAGTAA CTTCCTCTGT GTCAGCCACA TTTGGGAAGT CTGTTCAGAC ACAGCAGCCA
    TGCTCTGTCA AAGCCTCTTT GTCTTCTGAC ATCTGCTCCG GCTTAGGCCC CGAAGGAGGC
    GATGTGAATG CAAGCTCTGG CCAAGGCTGG ACGGTTTTCC ACCAAACGTC TGAGAGAGTG
    ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTTTGTCC
    ATCTGGTCCA CCTTGAAACG ACTATGTCTA GGCAAAGATC ACAGTAGTAG ATCCTCTACA
    AACAGTCTTG TAACGTGTCC TGAGCCCCTC CCGCAATCAA CTCTGCAGGT TCAGGCCAAC
    AACAGTAACA ACAAGAAGGG AACGTTCACC GATGATCTTC ACAAGCTGGT TGACCAGTGG
    ACAAGCAAAA CTGTTGGAGC TGCACAGGCA AAACCTTCTT TAAACCAACT GAAGCAGAAC
    CAAAAAAGGC AGGACATGGA GCCAAAGGCA AATCCCATGC AAACACAGAA TGAGACATGT
    GGAGTTAATT CAGTAAGAAC AGGATTGGGG AAAAGGTGCA TTGTTCCAGT GTCTTGTCCC
    ATGTCAGCTG CGCTAACTCC TGGAGTTTCA CCATCCATGC CAGTGCCTAT GCCAGGTACT
    ACCCCTTCTG GCGCAGTAAC GCCCTGTGTG ATGCCTCTTT GTCAGTACGG AGGAGTTTTC
    CCTACGCCGC TTTATGGAGT GCAGTGGTCG GGGCCGGCAG TGCGGCCGGG GCTGGTAGGA
    GGGCAGAGCA TTGCTCATTT CTCAGCTCTC AGCAAAGCTT GCTTGCAGAT GTACCCTGTG
    CCGCTGCAGC AGCCCGCAGC CGACCCGCCC GGCCCGCGTG TAAGAATGAC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148906.1
    CDS183..7136
    Translation

    Target ORF information:

    RefSeq Version XM_015293420.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGATCA
    GCTGCGAGCA TCACTCCAGC TCCTGTGACT CCAGCAAAGG GCCTCCTCCA GGTTGCACCT
    ACATCATCGA GCATAGCTAA ACAGATGGAA ACTTCTAAAA CGAACGAGAA GGCAAAGGCT
    ACAACTTCAT CTACGCACAC AGATAATGTA ATGCAGCTAT CGACAGCAGA TGGGGTGATA
    AATAACCTTC AAAGGGATGA CTCTCTAGAC TCCGGTTCCT ATGGAAAACA GGCTGGAACT
    CATGATAGCG GTACACCAGC CAAAACTATT GGCAGGTTTT CAGTAAGCAG CACGCAAGAT
    GAATTAACTT TAGCAGCGCC GCATTGCTTA AGATTCTCTG CGCCCCCGGA CGTCTATTTG
    GATGAGCTCC CTTCCAGCCC CGACCAGAAG ACAGCTGTCC GACGGGTGCA GACGGCCTCG
    TCTGTTGATG TCCTTTGTGA CCAGGGCTCG AGTGATTCTG CCGAGGAGCC CTTCCATCGA
    CAGGCTGCTG CGGCTCAGCC ATCCCCTCCG AGCAGTAGTG CAGCCTCTGA CTTGATTAAA
    AAAGCAGCAG CTTTTCTGCA AAGGACTGGA AAAGCAGGCT CCCAAGGCCC CGATTCACCA
    AACGGGCAGG GAACAAAGAT TCCTACCATC AACATAACAT CCTTCCACTC GCAGTCATCA
    TACATGAGCA GTGACAATGA CTCTGAATTT GAAGATGCAG ACATGAAGAA AGAACTGCAG
    AACTTGAGAG AGAAGCATAT GAAAGAAATT GCTGAACTTC AGACTCAGCA GAAGAATGAG
    ATAGAGGCGC TGTACATTCG GTTAGGGAAG CCCCTCCCTC CCAACATGGG GTTCCTTCAC
    TCAGCCCCGC CAAGCAGCCG TCGACGAAGA ACAAGCAAAA ATAAATTGAA AGGTGGAAAA
    CTATTAAACC CTCTGGTTCA ACAGCTCAGG AGTGGAACAT CAAGCACAAG TAATGTTTCA
    GACTCTAAAG ACTCACCCCA CAAAGAAACA ACTAAAGCTC AGCCTGGATC TCCCGAAACA
    ACAGGAGTAA CTTCCTCTGT GTCAGCCACA TTTGGGAAGT CTGTTCAGAC ACAGCAGCCA
    TGCTCTGTCA AAGCCTCTTT GTCTTCTGAC ATCTGCTCCG GCTTAGGCCC CGAAGGAGGC
    GATGTGAATG CAAGCTCTGG CCAAGGCTGG ACGGTTTTCC ACCAAACGTC TGAGAGAGTG
    ACCTATAAAT CTAGTAGCAA ACCTCGTACC AGATTCCTCA GTGGACCTGT GTCTTTGTCC
    ATCTGGTCCA CCTTGAAACG ACTATGTCTA GGCAAAGATC ACAGTAGTAG ATCCTCTACA
    AACAGTCTTG TAACGTGTCC TGAGCCCCTC CCGCAATCAA CTCTGCAGGT TCAGGCCAAC
    AACAGTAACA ACAAGAAGGG AACGTTCACC GATGATCTTC ACAAGCTGGT TGACCAGTGG
    ACAAGCAAAA CTGTTGGAGC TGCACAGGCA AAACCTTCTT TAAACCAACT GAAGCAGAAC
    CAAAAAAGGC AGGACATGGA GCCAAAGGCA AATCCCATGC AAACACAGAA TGAGACATGT
    GGAGTTAATT CAGTAAGAAC AGGATTGGGG AAAAGGTGCA TTGTTCCAGT GTCTTGTCCC
    ATGTCAGCTG CGCTAACTCC TGGAGTTTCA CCATCCATGC CAGTGCCTAT GCCAGGTACT
    ACCCCTTCTG GCGCAGTAAC GCCCTGTGTG ATGCCTCTTT GTCAGTACGG AGGAGTTTTC
    CCTACGCCGC TTTATGGAGT GCAGTGGTCG GGGCCGGCAG TGCGGCCGGG GCTGGTAGGA
    GGGCAGAGCA TTGCTCATTT CTCAGCTCTC AGCAAAGCTT GCTTGCAGAT GTACCCTGTG
    CCGCTGCAGC AGCCCGCAGC CGACCCGCCC GGCCCGCGTG TAAGAATGAC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41675
    Clone ID Related Accession (Same CDS sequence) XM_015293421.1
    Accession Version XM_015293421.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGGTC CACCTTGAAA
    CGACTATGTC TAGGCAAAGA TCACAGTAGT AGATCCTCTA CAAACAGTCT TGTAACGTGT
    CCTGAGCCCC TCCCGCAATC AACTCTGCAG GTTCAGGCCA ACAACAGTAA CAACAAGAAG
    GGAACGTTCA CCGATGATCT TCACAAGCTG GTTGACCAGT GGACAAGCAA AACTGTTGGA
    GCTGCACAGG CAAAACCTTC TTTAAACCAA CTGAAGCAGA ACCAAAAAAG GCAGGACATG
    GAGCCAAAGG CAAATCCCAT GCAAACACAG AATGAGACAT GTGGAGTTAA TTCAGTAAGA
    ACAGGATTGG GGAAAAGGTG CATTGTTCCA GTGTCTTGTC CCATGTCAGC TGCGCTAACT
    CCTGGAGTTT CACCATCCAT GCCAGTGCCT ATGCCAGGTA CTACCCCTTC TGGCGCAGTA
    ACGCCCTGTG TGATGCCTCT TTGTCAGTAC GGAGGAGTTT TCCCTACGCC GCTTTATGGA
    GTGCAGTGGT CGGGGCCGGC AGTGCGGCCG GGGCTGGTAG GAGGGCAGAG CATTGCTCAT
    TTCTCAGCTC TCAGCAAAGC TTGCTTGCAG ATGTACCCTG TGCCGCTGCA GCAGCCCGCA
    GCCGACCCGC CCGGCCCGCG TGTAAGAATG ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148907.1
    CDS183..7058
    Translation

    Target ORF information:

    RefSeq Version XM_015293421.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293421.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGGTC CACCTTGAAA
    CGACTATGTC TAGGCAAAGA TCACAGTAGT AGATCCTCTA CAAACAGTCT TGTAACGTGT
    CCTGAGCCCC TCCCGCAATC AACTCTGCAG GTTCAGGCCA ACAACAGTAA CAACAAGAAG
    GGAACGTTCA CCGATGATCT TCACAAGCTG GTTGACCAGT GGACAAGCAA AACTGTTGGA
    GCTGCACAGG CAAAACCTTC TTTAAACCAA CTGAAGCAGA ACCAAAAAAG GCAGGACATG
    GAGCCAAAGG CAAATCCCAT GCAAACACAG AATGAGACAT GTGGAGTTAA TTCAGTAAGA
    ACAGGATTGG GGAAAAGGTG CATTGTTCCA GTGTCTTGTC CCATGTCAGC TGCGCTAACT
    CCTGGAGTTT CACCATCCAT GCCAGTGCCT ATGCCAGGTA CTACCCCTTC TGGCGCAGTA
    ACGCCCTGTG TGATGCCTCT TTGTCAGTAC GGAGGAGTTT TCCCTACGCC GCTTTATGGA
    GTGCAGTGGT CGGGGCCGGC AGTGCGGCCG GGGCTGGTAG GAGGGCAGAG CATTGCTCAT
    TTCTCAGCTC TCAGCAAAGC TTGCTTGCAG ATGTACCCTG TGCCGCTGCA GCAGCCCGCA
    GCCGACCCGC CCGGCCCGCG TGTAAGAATG ACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41676
    Clone ID Related Accession (Same CDS sequence) XM_015293423.2 , XM_015293422.1 , XM_015293423.1 , XM_015293422.2
    Accession Version XM_015293423.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148909.1
    CDS202..7056
    Translation

    Target ORF information:

    RefSeq Version XM_015293423.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293423.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41676
    Clone ID Related Accession (Same CDS sequence) XM_015293423.2 , XM_015293422.1 , XM_015293423.1 , XM_015293422.2
    Accession Version XM_015293422.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148908.1
    CDS183..7037
    Translation

    Target ORF information:

    RefSeq Version XM_015293422.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41676
    Clone ID Related Accession (Same CDS sequence) XM_015293423.2 , XM_015293422.1 , XM_015293423.1 , XM_015293422.2
    Accession Version XM_015293423.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148909.1
    CDS183..7037
    Translation

    Target ORF information:

    RefSeq Version XM_015293423.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41676
    Clone ID Related Accession (Same CDS sequence) XM_015293423.2 , XM_015293422.1 , XM_015293423.1 , XM_015293422.2
    Accession Version XM_015293422.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148908.1
    CDS202..7056
    Translation

    Target ORF information:

    RefSeq Version XM_015293422.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293422.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGTTTG CTCCATAGGC AGCACGGACA CCTCTCTCCT GTTTGCGGGT
    TGCTTTTTGC CTCTTTTACT GTGCTTAGAG TCTGCATCTG CACCTCTCAG TCTAAAGGCA
    CAAGGTCACC ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41677
    Clone ID Related Accession (Same CDS sequence) XM_015293424.1
    Accession Version XM_015293424.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    GTTAATTCAG TAAGAACAGG ATTGGGGAAA AGGTGCATTG TTCCAGTGTC TTGTCCCATG
    TCAGCTGCGC TAACTCCTGG AGTTTCACCA TCCATGCCAG TGCCTATGCC AGGTACTACC
    CCTTCTGGCG CAGTAACGCC CTGTGTGATG CCTCTTTGTC AGTACGGAGG AGTTTTCCCT
    ACGCCGCTTT ATGGAGTGCA GTGGTCGGGG CCGGCAGTGC GGCCGGGGCT GGTAGGAGGG
    CAGAGCATTG CTCATTTCTC AGCTCTCAGC AAAGCTTGCT TGCAGATGTA CCCTGTGCCG
    CTGCAGCAGC CCGCAGCCGA CCCGCCCGGC CCGCGTGTAA GAATGACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148910.1
    CDS183..7013
    Translation

    Target ORF information:

    RefSeq Version XM_015293424.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293424.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    GTTAATTCAG TAAGAACAGG ATTGGGGAAA AGGTGCATTG TTCCAGTGTC TTGTCCCATG
    TCAGCTGCGC TAACTCCTGG AGTTTCACCA TCCATGCCAG TGCCTATGCC AGGTACTACC
    CCTTCTGGCG CAGTAACGCC CTGTGTGATG CCTCTTTGTC AGTACGGAGG AGTTTTCCCT
    ACGCCGCTTT ATGGAGTGCA GTGGTCGGGG CCGGCAGTGC GGCCGGGGCT GGTAGGAGGG
    CAGAGCATTG CTCATTTCTC AGCTCTCAGC AAAGCTTGCT TGCAGATGTA CCCTGTGCCG
    CTGCAGCAGC CCGCAGCCGA CCCGCCCGGC CCGCGTGTAA GAATGACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41678
    Clone ID Related Accession (Same CDS sequence) XM_015293425.1
    Accession Version XM_015293425.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC AACCCATATC ACAGCAAACT
    TCTCTGCAGC AGGTGCTTGC CAGCCAGCCG GTTTGCCCCA TCCAGCCTGC CCCCCATCTC
    CTGCCACAGT ATCAGCCCCA GACCTCTCAG GTGGCAGCCA GTAGTACACC ACTGAAACCC
    CTTCAGATTT CAACTGTGCC ACAGCTTCCT CCAGTTGCCC CTTCCCAGAT TCCTCAGTTT
    CCCGTCATTC CTGCAATTAC TCCTCTGGCA GGACTTGACA ACCTTCCTCC AAACCTCTCT
    GATATACCTG CTGCAAACGT GCCCCCCATA CCTGCTCCTT CTCAGTATTT TGCTCCAGCT
    GTGATTTTAC CGAGCCTCCC CATGAACCCC ACTTTGCCCA TGGCACCAAA CTCCCCTGCC
    CTCCCCATGC AAGCAGTGAA CCTTCCTCAT ACAACTGTGG CTTCTCTGGT CTTGCCCTGC
    CAAACAATAG TGCCAAATAT GTCGGCTGCT ACAATACCGT TGCTTGCTGT GGCTCCGCCG
    GGAGTGCCAG CGCTGCCGCC GCACCACGCG GTGTCCCAGC TGCCCCAGCA GCAGATGTAC
    CCGACCACCT TCCAGCAGAT AATCCAGAGC GAAGCGCCCT CTCCCCATCA CACGCAGAGC
    ACGCAAGCCG TTTCCCAGCC GCAGCAGCCT CCACCTCCTC AGTCACTTCA GCCAGGCGTA
    ATTCATCCAT CAGAACAGCC GTTGTCCGCC CCTGGGGCTG GTAACCAGCA GGTTACTGGA
    CATACTCAGT ATGGTTTGGA AACTGGAGCC CAGGTGCCGG TACTCCCAGT GCAGCCTTCG
    CTAGTCATGT CACCGCCTTT GCAGTTGCAA CCTGAACTAT TGCCTCCCCG CGTCGCTCCA
    GAAAGTATTT CACAGATTCA TGGCAGCCTT GCTACAGCTA GTCCACCAGT GCCCATAGAT
    CACTGTCTGC CTCTCCAGCC TTCGGGTGTT CTGCAGCTTC AGCCAGACCT TTCCCAGCCT
    CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA GCCGTGCAGG CAGTGGCAGA GACATCGCAA
    GAGGAGCAGG CAATACAGGA CAAACTTCAA ACTCTGTCAC AGAGCTGTGA AAGCTACATG
    AGTCCAGATG TCGCTTCAGG TAAAGAGATG AGTGACAGCT TTGAAGGAGC AATAGGAAGT
    GGAAAACAAG AAGGAAAGTC TTCAAAGAAA CATAAGAAAT CTACACGTGC TCGTTCACGC
    CAGGAGAAAA CCAACAGACC CAAACTGACT ATATTAAATG TTTGTAACAC AGGGGATAAG
    ATGGTGGAGT GCCAGCTTGA AACACACAAT CACAAAATGG TGACTTTTAA GTTTGATTTG
    GATGGTGATG CGCCTGAGGA AATTGCTACT TACATGGTAG AGAATGAATT CATATTGCAG
    AGTGAGAAAG AGACGTTTAT TGAACAAATG AAAGATATTA TCGATAAAGC AGAAGATATG
    CTCAGTGAAG ATACAGAAGG AGAACGGAGT TCTGATCAGG GAACAAGTCC CCAGCAGGAT
    ACTGATGGAG CAGAAATGAG TGAGGAGAAG CGGCCATCTC AAGTGAAGAC ACCCGTGTAT
    CAACAAAATG TTTTGCATAC CGGAAAAAGG TGGTTTATTA TATGCCCTGT GGTGGAAAAC
    CCGACTACTG ATGCACCAGA ATTTTCTCCA CCAGTTCCTC AGAGTGCACA ACAGTCTGAA
    GGTCCAGACC TGGAGCAGTC GGATGATCAG CAAGCAAACT CTGCGGTACC GGATACGCCT
    GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA CAAGCAAGCG TCTGTGACAG CCCCATTGGG
    GCCTCTCCAT CTTTGGCACA AATTCCTGCT GCAGCATCTT CAGCTGGCTT GCCAAGCCAG
    GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA CCTGCTCCAA ACATCCTGGA GCAGGCAGCC
    GCTAACGGAG GCCAGCCGGA ATGTTCTCTA CTGAAAGCAG CATTGCCCGC CCCACCTGGC
    ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG GAGCCCGCTG ATGCTCCACT GGCTGCTCAG
    CCTACTCAGC ATGCGCAGCA GCAGGCAGAC GAGGCCATGT GTATCCCTGA TGTGGAGATA
    GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA GTCCCACCAG CTGCTGCCAA GGCACCGGAG
    CTCTGCCCGC TCCCTGTGCT CTCAGATGCT GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG
    ATCCCTCACC TGCCAGAGGC CAGCCAGCCC GGCGTGGTGC AGCACTCCTT TGTGAACAAA
    GTGTTTCCTC CATCTGTTGC ATCTGAGTCT CTCAATCTGG CAGGATCTCA ACTTCAGAGC
    CCCACTCAGG TGTCCATGGC CGCATCCCAG CAGCACGCGA TGCTGTCCCA GACACAGCCT
    GTCACCTGCG CCAGCGTGGG GATCAGCCTG CTGCAGCAGC AGCAACAGCA GCAGCCGTGC
    ACCGTGGAGT CTGATGGAGA GGGGCCACCT CGGGTGGACT TTGTTGACAA CACAATAAAA
    AGCCTCGATG AGAAACTGCG CAACTTACTT TACCAGGAAT ATGTCCCAAC TTCTTCTGCA
    TCAGCAGGAA CTCCAGACGC TTTTGTGCCA TTGGAACAGG CAGACAGTGA ATTTAACTTG
    CCACCACTTA CCGAAGATCA AGTTCCCAAG TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT
    AACACCACGG ATAGCTGCCA GGAGGTGAAA GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG
    CCACCTGAGA TCGCACCTTA TCCAGCATTG CTTGAAAAGT CTGAGGTCGC TGGCATTGGG
    ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA GCAAGCCAAA GAGGATCAGC TGCGAGCATC
    ACTCCAGCTC CTGTGACTCC AGCAAAGGGC CTCCTCCAGG TTGCACCTAC ATCATCGAGC
    ATAGCTAAAC AGATGGAAAC TTCTAAAACG AACGAGAAGG CAAAGGCTAC AACTTCATCT
    ACGCACACAG ATAATGTAAT GCAGCTATCG ACAGCAGATG GGGTGATAAA TAACCTTCAA
    AGGGATGACT CTCTAGACTC CGGTTCCTAT GGAAAACAGG CTGGAACTCA TGATAGCGGT
    ACACCAGCCA AAACTATTGG CAGGTTTTCA GTAAGCAGCA CGCAAGATGA ATTAACTTTA
    GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG CCCCCGGACG TCTATTTGGA TGAGCTCCCT
    TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA CGGGTGCAGA CGGCCTCGTC TGTTGATGTC
    CTTTGTGACC AGGGCTCGAG TGATTCTGCC GAGGAGCCCT TCCATCGACA GGCTGCTGCG
    GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA GCCTCTGACT TGATTAAAAA AGCAGCAGCT
    TTTCTGCAAA GGACTGGAAA AGCAGGCTCC CAAGGCCCCG ATTCACCAAA CGGGCAGGGA
    ACAAAGATTC CTACCATCAA CATAACATCC TTCCACTCGC AGTCATCATA CATGAGCAGT
    GACAATGACT CTGAATTTGA AGATGCAGAC ATGAAGAAAG AACTGCAGAA CTTGAGAGAG
    AAGCATATGA AAGAAATTGC TGAACTTCAG ACTCAGCAGA AGAATGAGAT AGAGGCGCTG
    TACATTCGGT TAGGGAAGCC CCTCCCTCCC AACATGGGGT TCCTTCACTC AGCCCCGCCA
    AGCAGCCGTC GACGAAGAAC AAGCAAAAAT AAATTGAAAG GTGGAAAACT ATTAAACCCT
    CTGGTTCAAC AGCTCAGGAG TGGAACATCA AGCACAAGTA ATGTTTCAGA CTCTAAAGAC
    TCACCCCACA AAGAAACAAC TAAAGCTCAG CCTGGATCTC CCGAAACAAC AGGAGTAACT
    TCCTCTGTGT CAGCCACATT TGGGAAGTCT GTTCAGACAC AGCAGCCATG CTCTGTCAAA
    GCCTCTTTGT CTTCTGACAT CTGCTCCGGC TTAGGCCCCG AAGGAGGCGA TGTGAATGCA
    AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC CAAACGTCTG AGAGAGTGAC CTATAAATCT
    AGTAGCAAAC CTCGTACCAG ATTCCTCAGT GGACCTGTGT CTTTGTCCAT CTGGTCCACC
    TTGAAACGAC TATGTCTAGG CAAAGATCAC AGTAGTAGAT CCTCTACAAA CAGTCTTGTA
    ACGTGTCCTG AGCCCCTCCC GCAATCAACT CTGCAGGTTC AGGCCAACAA CAGTAACAAC
    AAGAAGGGAA CGTTCACCGA TGATCTTCAC AAGCTGGTTG ACCAGTGGAC AAGCAAAACT
    GTTGGAGCTG CACAGGCAAA ACCTTCTTTA AACCAACTGA AGCAGAACCA AAAAAGGCAG
    GACATGGAGC CAAAGGCAAA TCCCATGCAA ACACAGAATG AGACATGTGG AGTTAATTCA
    GTAAGAACAG GATTGGGGAA AAGGTGCATT GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG
    CTAACTCCTG GAGTTTCACC ATCCATGCCA GTGCCTATGC CAGGTACTAC CCCTTCTGGC
    GCAGTAACGC CCTGTGTGAT GCCTCTTTGT CAGTACGGAG GAGTTTTCCC TACGCCGCTT
    TATGGAGTGC AGTGGTCGGG GCCGGCAGTG CGGCCGGGGC TGGTAGGAGG GCAGAGCATT
    GCTCATTTCT CAGCTCTCAG CAAAGCTTGC TTGCAGATGT ACCCTGTGCC GCTGCAGCAG
    CCCGCAGCCG ACCCGCCCGG CCCGCGTGTA AGAATGACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148911.1
    CDS184..7005
    Translation

    Target ORF information:

    RefSeq Version XM_015293425.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC AACCCATATC ACAGCAAACT
    TCTCTGCAGC AGGTGCTTGC CAGCCAGCCG GTTTGCCCCA TCCAGCCTGC CCCCCATCTC
    CTGCCACAGT ATCAGCCCCA GACCTCTCAG GTGGCAGCCA GTAGTACACC ACTGAAACCC
    CTTCAGATTT CAACTGTGCC ACAGCTTCCT CCAGTTGCCC CTTCCCAGAT TCCTCAGTTT
    CCCGTCATTC CTGCAATTAC TCCTCTGGCA GGACTTGACA ACCTTCCTCC AAACCTCTCT
    GATATACCTG CTGCAAACGT GCCCCCCATA CCTGCTCCTT CTCAGTATTT TGCTCCAGCT
    GTGATTTTAC CGAGCCTCCC CATGAACCCC ACTTTGCCCA TGGCACCAAA CTCCCCTGCC
    CTCCCCATGC AAGCAGTGAA CCTTCCTCAT ACAACTGTGG CTTCTCTGGT CTTGCCCTGC
    CAAACAATAG TGCCAAATAT GTCGGCTGCT ACAATACCGT TGCTTGCTGT GGCTCCGCCG
    GGAGTGCCAG CGCTGCCGCC GCACCACGCG GTGTCCCAGC TGCCCCAGCA GCAGATGTAC
    CCGACCACCT TCCAGCAGAT AATCCAGAGC GAAGCGCCCT CTCCCCATCA CACGCAGAGC
    ACGCAAGCCG TTTCCCAGCC GCAGCAGCCT CCACCTCCTC AGTCACTTCA GCCAGGCGTA
    ATTCATCCAT CAGAACAGCC GTTGTCCGCC CCTGGGGCTG GTAACCAGCA GGTTACTGGA
    CATACTCAGT ATGGTTTGGA AACTGGAGCC CAGGTGCCGG TACTCCCAGT GCAGCCTTCG
    CTAGTCATGT CACCGCCTTT GCAGTTGCAA CCTGAACTAT TGCCTCCCCG CGTCGCTCCA
    GAAAGTATTT CACAGATTCA TGGCAGCCTT GCTACAGCTA GTCCACCAGT GCCCATAGAT
    CACTGTCTGC CTCTCCAGCC TTCGGGTGTT CTGCAGCTTC AGCCAGACCT TTCCCAGCCT
    CTGGCTCAGC AGCTCCCCGC TCCCGGCTCA GCCGTGCAGG CAGTGGCAGA GACATCGCAA
    GAGGAGCAGG CAATACAGGA CAAACTTCAA ACTCTGTCAC AGAGCTGTGA AAGCTACATG
    AGTCCAGATG TCGCTTCAGG TAAAGAGATG AGTGACAGCT TTGAAGGAGC AATAGGAAGT
    GGAAAACAAG AAGGAAAGTC TTCAAAGAAA CATAAGAAAT CTACACGTGC TCGTTCACGC
    CAGGAGAAAA CCAACAGACC CAAACTGACT ATATTAAATG TTTGTAACAC AGGGGATAAG
    ATGGTGGAGT GCCAGCTTGA AACACACAAT CACAAAATGG TGACTTTTAA GTTTGATTTG
    GATGGTGATG CGCCTGAGGA AATTGCTACT TACATGGTAG AGAATGAATT CATATTGCAG
    AGTGAGAAAG AGACGTTTAT TGAACAAATG AAAGATATTA TCGATAAAGC AGAAGATATG
    CTCAGTGAAG ATACAGAAGG AGAACGGAGT TCTGATCAGG GAACAAGTCC CCAGCAGGAT
    ACTGATGGAG CAGAAATGAG TGAGGAGAAG CGGCCATCTC AAGTGAAGAC ACCCGTGTAT
    CAACAAAATG TTTTGCATAC CGGAAAAAGG TGGTTTATTA TATGCCCTGT GGTGGAAAAC
    CCGACTACTG ATGCACCAGA ATTTTCTCCA CCAGTTCCTC AGAGTGCACA ACAGTCTGAA
    GGTCCAGACC TGGAGCAGTC GGATGATCAG CAAGCAAACT CTGCGGTACC GGATACGCCT
    GGTGTCTTAG CTTGTCAGCA GCTTCCTCTA CAAGCAAGCG TCTGTGACAG CCCCATTGGG
    GCCTCTCCAT CTTTGGCACA AATTCCTGCT GCAGCATCTT CAGCTGGCTT GCCAAGCCAG
    GCAGAGCTCT CAGTTCCAGC ACCGCCAGGA CCTGCTCCAA ACATCCTGGA GCAGGCAGCC
    GCTAACGGAG GCCAGCCGGA ATGTTCTCTA CTGAAAGCAG CATTGCCCGC CCCACCTGGC
    ACTCCTGTCC CAGGTGCTGT CCCTCTGGAG GAGCCCGCTG ATGCTCCACT GGCTGCTCAG
    CCTACTCAGC ATGCGCAGCA GCAGGCAGAC GAGGCCATGT GTATCCCTGA TGTGGAGATA
    GCGTGTTGCA GTGTCGGGCC ACCCAAAGCA GTCCCACCAG CTGCTGCCAA GGCACCGGAG
    CTCTGCCCGC TCCCTGTGCT CTCAGATGCT GTTGTTTTGG AGCGGCAGCC TGCGGCCCAG
    ATCCCTCACC TGCCAGAGGC CAGCCAGCCC GGCGTGGTGC AGCACTCCTT TGTGAACAAA
    GTGTTTCCTC CATCTGTTGC ATCTGAGTCT CTCAATCTGG CAGGATCTCA ACTTCAGAGC
    CCCACTCAGG TGTCCATGGC CGCATCCCAG CAGCACGCGA TGCTGTCCCA GACACAGCCT
    GTCACCTGCG CCAGCGTGGG GATCAGCCTG CTGCAGCAGC AGCAACAGCA GCAGCCGTGC
    ACCGTGGAGT CTGATGGAGA GGGGCCACCT CGGGTGGACT TTGTTGACAA CACAATAAAA
    AGCCTCGATG AGAAACTGCG CAACTTACTT TACCAGGAAT ATGTCCCAAC TTCTTCTGCA
    TCAGCAGGAA CTCCAGACGC TTTTGTGCCA TTGGAACAGG CAGACAGTGA ATTTAACTTG
    CCACCACTTA CCGAAGATCA AGTTCCCAAG TTGGTGTTGG ATTTGAGAGA ACCAGGCCAT
    AACACCACGG ATAGCTGCCA GGAGGTGAAA GCTGCCGAGG CCCAGTTTGT GCCGGCTGTG
    CCACCTGAGA TCGCACCTTA TCCAGCATTG CTTGAAAAGT CTGAGGTCGC TGGCATTGGG
    ACTGAGTCTT TGGAAGCAAG AGGTGGGTCA GCAAGCCAAA GAGGATCAGC TGCGAGCATC
    ACTCCAGCTC CTGTGACTCC AGCAAAGGGC CTCCTCCAGG TTGCACCTAC ATCATCGAGC
    ATAGCTAAAC AGATGGAAAC TTCTAAAACG AACGAGAAGG CAAAGGCTAC AACTTCATCT
    ACGCACACAG ATAATGTAAT GCAGCTATCG ACAGCAGATG GGGTGATAAA TAACCTTCAA
    AGGGATGACT CTCTAGACTC CGGTTCCTAT GGAAAACAGG CTGGAACTCA TGATAGCGGT
    ACACCAGCCA AAACTATTGG CAGGTTTTCA GTAAGCAGCA CGCAAGATGA ATTAACTTTA
    GCAGCGCCGC ATTGCTTAAG ATTCTCTGCG CCCCCGGACG TCTATTTGGA TGAGCTCCCT
    TCCAGCCCCG ACCAGAAGAC AGCTGTCCGA CGGGTGCAGA CGGCCTCGTC TGTTGATGTC
    CTTTGTGACC AGGGCTCGAG TGATTCTGCC GAGGAGCCCT TCCATCGACA GGCTGCTGCG
    GCTCAGCCAT CCCCTCCGAG CAGTAGTGCA GCCTCTGACT TGATTAAAAA AGCAGCAGCT
    TTTCTGCAAA GGACTGGAAA AGCAGGCTCC CAAGGCCCCG ATTCACCAAA CGGGCAGGGA
    ACAAAGATTC CTACCATCAA CATAACATCC TTCCACTCGC AGTCATCATA CATGAGCAGT
    GACAATGACT CTGAATTTGA AGATGCAGAC ATGAAGAAAG AACTGCAGAA CTTGAGAGAG
    AAGCATATGA AAGAAATTGC TGAACTTCAG ACTCAGCAGA AGAATGAGAT AGAGGCGCTG
    TACATTCGGT TAGGGAAGCC CCTCCCTCCC AACATGGGGT TCCTTCACTC AGCCCCGCCA
    AGCAGCCGTC GACGAAGAAC AAGCAAAAAT AAATTGAAAG GTGGAAAACT ATTAAACCCT
    CTGGTTCAAC AGCTCAGGAG TGGAACATCA AGCACAAGTA ATGTTTCAGA CTCTAAAGAC
    TCACCCCACA AAGAAACAAC TAAAGCTCAG CCTGGATCTC CCGAAACAAC AGGAGTAACT
    TCCTCTGTGT CAGCCACATT TGGGAAGTCT GTTCAGACAC AGCAGCCATG CTCTGTCAAA
    GCCTCTTTGT CTTCTGACAT CTGCTCCGGC TTAGGCCCCG AAGGAGGCGA TGTGAATGCA
    AGCTCTGGCC AAGGCTGGAC GGTTTTCCAC CAAACGTCTG AGAGAGTGAC CTATAAATCT
    AGTAGCAAAC CTCGTACCAG ATTCCTCAGT GGACCTGTGT CTTTGTCCAT CTGGTCCACC
    TTGAAACGAC TATGTCTAGG CAAAGATCAC AGTAGTAGAT CCTCTACAAA CAGTCTTGTA
    ACGTGTCCTG AGCCCCTCCC GCAATCAACT CTGCAGGTTC AGGCCAACAA CAGTAACAAC
    AAGAAGGGAA CGTTCACCGA TGATCTTCAC AAGCTGGTTG ACCAGTGGAC AAGCAAAACT
    GTTGGAGCTG CACAGGCAAA ACCTTCTTTA AACCAACTGA AGCAGAACCA AAAAAGGCAG
    GACATGGAGC CAAAGGCAAA TCCCATGCAA ACACAGAATG AGACATGTGG AGTTAATTCA
    GTAAGAACAG GATTGGGGAA AAGGTGCATT GTTCCAGTGT CTTGTCCCAT GTCAGCTGCG
    CTAACTCCTG GAGTTTCACC ATCCATGCCA GTGCCTATGC CAGGTACTAC CCCTTCTGGC
    GCAGTAACGC CCTGTGTGAT GCCTCTTTGT CAGTACGGAG GAGTTTTCCC TACGCCGCTT
    TATGGAGTGC AGTGGTCGGG GCCGGCAGTG CGGCCGGGGC TGGTAGGAGG GCAGAGCATT
    GCTCATTTCT CAGCTCTCAG CAAAGCTTGC TTGCAGATGT ACCCTGTGCC GCTGCAGCAG
    CCCGCAGCCG ACCCGCCCGG CCCGCGTGTA AGAATGACAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41679
    Clone ID Related Accession (Same CDS sequence) XM_015293426.1 , XM_015293426.2
    Accession Version XM_015293426.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGATTC TGAAAATGCG ATCGCCAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148912.1
    CDS183..6947
    Translation

    Target ORF information:

    RefSeq Version XM_015293426.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293426.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGATTC TGAAAATGCG ATCGCCAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41679
    Clone ID Related Accession (Same CDS sequence) XM_015293426.1 , XM_015293426.2
    Accession Version XM_015293426.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6765bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293426.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGATTC TGAAAATGCG ATCGCCAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148912.1
    CDS202..6966
    Translation

    Target ORF information:

    RefSeq Version XM_015293426.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293426.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGATTC TGAAAATGCG ATCGCCAGCG ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41680
    Clone ID Related Accession (Same CDS sequence) XM_015293427.1 , XM_015293427.2
    Accession Version XM_015293427.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAATTCTG AAAATGCGAT CGCCAGCGAC TGACACGTGG
    CCCCCGCAGC TACGGTGCCA AGCGCCAGGT CCTGCTGCAG CTAAGGAGGA GGAAGAGGAA
    GATGGGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148913.1
    CDS182..6913
    Translation

    Target ORF information:

    RefSeq Version XM_015293427.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293427.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAATTCTG AAAATGCGAT CGCCAGCGAC TGACACGTGG
    CCCCCGCAGC TACGGTGCCA AGCGCCAGGT CCTGCTGCAG CTAAGGAGGA GGAAGAGGAA
    GATGGGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41680
    Clone ID Related Accession (Same CDS sequence) XM_015293427.1 , XM_015293427.2
    Accession Version XM_015293427.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6732bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAATTCTG AAAATGCGAT CGCCAGCGAC TGACACGTGG
    CCCCCGCAGC TACGGTGCCA AGCGCCAGGT CCTGCTGCAG CTAAGGAGGA GGAAGAGGAA
    GATGGGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148913.1
    CDS201..6932
    Translation

    Target ORF information:

    RefSeq Version XM_015293427.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293427.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGGTCC ACCTTGAAAC GACTATGTCT AGGCAAAGAT
    CACAGTAGTA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAATTCTG AAAATGCGAT CGCCAGCGAC TGACACGTGG
    CCCCCGCAGC TACGGTGCCA AGCGCCAGGT CCTGCTGCAG CTAAGGAGGA GGAAGAGGAA
    GATGGGAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41681
    Clone ID Related Accession (Same CDS sequence) XM_015293428.1
    Accession Version XM_015293428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGAGCAGGCA ATACAGGACA AACTTCAAAC TCTGTCACAG
    AGCTGTGAAA GCTACATGAG TCCAGATGTC GCTTCAGGTA AAGAGATGAG TGACAGCTTT
    GAAGGAGCAA TAGGAAGTGG AAAACAAGAA GGAAAGTCTT CAAAGAAACA TAAGAAATCT
    ACACGTGCTC GTTCACGCCA GGAGAAAACC AACAGACCCA AACTGACTAT ATTAAATGTT
    TGTAACACAG GGGATAAGAT GGTGGAGTGC CAGCTTGAAA CACACAATCA CAAAATGGTG
    ACTTTTAAGT TTGATTTGGA TGGTGATGCG CCTGAGGAAA TTGCTACTTA CATGGTAGAG
    AATGAATTCA TATTGCAGAG TGAGAAAGAG ACGTTTATTG AACAAATGAA AGATATTATC
    GATAAAGCAG AAGATATGCT CAGTGAAGAT ACAGAAGGAG AACGGAGTTC TGATCAGGGA
    ACAAGTCCCC AGCAGGATAC TGATGGAGCA GAAATGAGTG AGGAGAAGCG GCCATCTCAA
    GTGAAGACAC CCGTGTATCA ACAAAATGTT TTGCATACCG GAAAAAGGTG GTTTATTATA
    TGCCCTGTGG TGGAAAACCC GACTACTGAT GCACCAGAAT TTTCTCCACC AGTTCCTCAG
    AGTGCACAAC AGTCTGAAGG TCCAGACCTG GAGCAGTCGG ATGATCAGCA AGCAAACTCT
    GCGGTACCGG ATACGCCTGG TGTCTTAGCT TGTCAGCAGC TTCCTCTACA AGCAAGCGTC
    TGTGACAGCC CCATTGGGGC CTCTCCATCT TTGGCACAAA TTCCTGCTGC AGCATCTTCA
    GCTGGCTTGC CAAGCCAGGC AGAGCTCTCA GTTCCAGCAC CGCCAGGACC TGCTCCAAAC
    ATCCTGGAGC AGGCAGCCGC TAACGGAGGC CAGCCGGAAT GTTCTCTACT GAAAGCAGCA
    TTGCCCGCCC CACCTGGCAC TCCTGTCCCA GGTGCTGTCC CTCTGGAGGA GCCCGCTGAT
    GCTCCACTGG CTGCTCAGCC TACTCAGCAT GCGCAGCAGC AGGCAGACGA GGCCATGTGT
    ATCCCTGATG TGGAGATAGC GTGTTGCAGT GTCGGGCCAC CCAAAGCAGT CCCACCAGCT
    GCTGCCAAGG CACCGGAGCT CTGCCCGCTC CCTGTGCTCT CAGATGCTGT TGTTTTGGAG
    CGGCAGCCTG CGGCCCAGAT CCCTCACCTG CCAGAGGCCA GCCAGCCCGG CGTGGTGCAG
    CACTCCTTTG TGAACAAAGT GTTTCCTCCA TCTGTTGCAT CTGAGTCTCT CAATCTGGCA
    GGATCTCAAC TTCAGAGCCC CACTCAGGTG TCCATGGCCG CATCCCAGCA GCACGCGATG
    CTGTCCCAGA CACAGCCTGT CACCTGCGCC AGCGTGGGGA TCAGCCTGCT GCAGCAGCAG
    CAACAGCAGC AGCCGTGCAC CGTGGAGTCT GATGGAGAGG GGCCACCTCG GGTGGACTTT
    GTTGACAACA CAATAAAAAG CCTCGATGAG AAACTGCGCA ACTTACTTTA CCAGGAATAT
    GTCCCAACTT CTTCTGCATC AGCAGGAACT CCAGACGCTT TTGTGCCATT GGAACAGGCA
    GACAGTGAAT TTAACTTGCC ACCACTTACC GAAGATCAAG TTCCCAAGTT GGTGTTGGAT
    TTGAGAGAAC CAGGCCATAA CACCACGGAT AGCTGCCAGG AGGTGAAAGC TGCCGAGGCC
    CAGTTTGTGC CGGCTGTGCC ACCTGAGATC GCACCTTATC CAGCATTGCT TGAAAAGTCT
    GAGGTCGCTG GCATTGGGAC TGAGTCTTTG GAAGCAAGAG GTGGGTCAGC AAGCCAAAGA
    GGATCAGCTG CGAGCATCAC TCCAGCTCCT GTGACTCCAG CAAAGGGCCT CCTCCAGGTT
    GCACCTACAT CATCGAGCAT AGCTAAACAG ATGGAAACTT CTAAAACGAA CGAGAAGGCA
    AAGGCTACAA CTTCATCTAC GCACACAGAT AATGTAATGC AGCTATCGAC AGCAGATGGG
    GTGATAAATA ACCTTCAAAG GGATGACTCT CTAGACTCCG GTTCCTATGG AAAACAGGCT
    GGAACTCATG ATAGCGGTAC ACCAGCCAAA ACTATTGGCA GGTTTTCAGT AAGCAGCACG
    CAAGATGAAT TAACTTTAGC AGCGCCGCAT TGCTTAAGAT TCTCTGCGCC CCCGGACGTC
    TATTTGGATG AGCTCCCTTC CAGCCCCGAC CAGAAGACAG CTGTCCGACG GGTGCAGACG
    GCCTCGTCTG TTGATGTCCT TTGTGACCAG GGCTCGAGTG ATTCTGCCGA GGAGCCCTTC
    CATCGACAGG CTGCTGCGGC TCAGCCATCC CCTCCGAGCA GTAGTGCAGC CTCTGACTTG
    ATTAAAAAAG CAGCAGCTTT TCTGCAAAGG ACTGGAAAAG CAGGCTCCCA AGGCCCCGAT
    TCACCAAACG GGCAGGGAAC AAAGATTCCT ACCATCAACA TAACATCCTT CCACTCGCAG
    TCATCATACA TGAGCAGTGA CAATGACTCT GAATTTGAAG ATGCAGACAT GAAGAAAGAA
    CTGCAGAACT TGAGAGAGAA GCATATGAAA GAAATTGCTG AACTTCAGAC TCAGCAGAAG
    AATGAGATAG AGGCGCTGTA CATTCGGTTA GGGAAGCCCC TCCCTCCCAA CATGGGGTTC
    CTTCACTCAG CCCCGCCAAG CAGCCGTCGA CGAAGAACAA GCAAAAATAA ATTGAAAGGT
    GGAAAACTAT TAAACCCTCT GGTTCAACAG CTCAGGAGTG GAACATCAAG CACAAGTAAT
    GTTTCAGACT CTAAAGACTC ACCCCACAAA GAAACAACTA AAGCTCAGCC TGGATCTCCC
    GAAACAACAG GAGTAACTTC CTCTGTGTCA GCCACATTTG GGAAGTCTGT TCAGACACAG
    CAGCCATGCT CTGTCAAAGC CTCTTTGTCT TCTGACATCT GCTCCGGCTT AGGCCCCGAA
    GGAGGCGATG TGAATGCAAG CTCTGGCCAA GGCTGGACGG TTTTCCACCA AACGTCTGAG
    AGAGTGACCT ATAAATCTAG TAGCAAACCT CGTACCAGAT TCCTCAGTGG ACCTGTGTCT
    TTGTCCATCT GGTCCACCTT GAAACGACTA TGTCTAGGCA AAGATCACAG TAGTAGATCC
    TCTACAAACA GTCTTGTAAC GTGTCCTGAG CCCCTCCCGC AATCAACTCT GCAGGTTCAG
    GCCAACAACA GTAACAACAA GAAGGGAACG TTCACCGATG ATCTTCACAA GCTGGTTGAC
    CAGTGGACAA GCAAAACTGT TGGAGCTGCA CAGGCAAAAC CTTCTTTAAA CCAACTGAAG
    CAGAACCAAA AAAGGCAGGA CATGGAGCCA AAGGCAAATC CCATGCAAAC ACAGAATGAG
    ACATGTGGAG TTAATTCAGT AAGAACAGGA TTGGGGAAAA GGTGCATTGT TCCAGTGTCT
    TGTCCCATGT CAGCTGCGCT AACTCCTGGA GTTTCACCAT CCATGCCAGT GCCTATGCCA
    GGTACTACCC CTTCTGGCGC AGTAACGCCC TGTGTGATGC CTCTTTGTCA GTACGGAGGA
    GTTTTCCCTA CGCCGCTTTA TGGAGTGCAG TGGTCGGGGC CGGCAGTGCG GCCGGGGCTG
    GTAGGAGGGC AGAGCATTGC TCATTTCTCA GCTCTCAGCA AAGCTTGCTT GCAGATGTAC
    CCTGTGCCGC TGCAGCAGCC CGCAGCCGAC CCGCCCGGCC CGCGTGTAAG AATGACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148914.1
    CDS183..6842
    Translation

    Target ORF information:

    RefSeq Version XM_015293428.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GGAGCAGGCA ATACAGGACA AACTTCAAAC TCTGTCACAG
    AGCTGTGAAA GCTACATGAG TCCAGATGTC GCTTCAGGTA AAGAGATGAG TGACAGCTTT
    GAAGGAGCAA TAGGAAGTGG AAAACAAGAA GGAAAGTCTT CAAAGAAACA TAAGAAATCT
    ACACGTGCTC GTTCACGCCA GGAGAAAACC AACAGACCCA AACTGACTAT ATTAAATGTT
    TGTAACACAG GGGATAAGAT GGTGGAGTGC CAGCTTGAAA CACACAATCA CAAAATGGTG
    ACTTTTAAGT TTGATTTGGA TGGTGATGCG CCTGAGGAAA TTGCTACTTA CATGGTAGAG
    AATGAATTCA TATTGCAGAG TGAGAAAGAG ACGTTTATTG AACAAATGAA AGATATTATC
    GATAAAGCAG AAGATATGCT CAGTGAAGAT ACAGAAGGAG AACGGAGTTC TGATCAGGGA
    ACAAGTCCCC AGCAGGATAC TGATGGAGCA GAAATGAGTG AGGAGAAGCG GCCATCTCAA
    GTGAAGACAC CCGTGTATCA ACAAAATGTT TTGCATACCG GAAAAAGGTG GTTTATTATA
    TGCCCTGTGG TGGAAAACCC GACTACTGAT GCACCAGAAT TTTCTCCACC AGTTCCTCAG
    AGTGCACAAC AGTCTGAAGG TCCAGACCTG GAGCAGTCGG ATGATCAGCA AGCAAACTCT
    GCGGTACCGG ATACGCCTGG TGTCTTAGCT TGTCAGCAGC TTCCTCTACA AGCAAGCGTC
    TGTGACAGCC CCATTGGGGC CTCTCCATCT TTGGCACAAA TTCCTGCTGC AGCATCTTCA
    GCTGGCTTGC CAAGCCAGGC AGAGCTCTCA GTTCCAGCAC CGCCAGGACC TGCTCCAAAC
    ATCCTGGAGC AGGCAGCCGC TAACGGAGGC CAGCCGGAAT GTTCTCTACT GAAAGCAGCA
    TTGCCCGCCC CACCTGGCAC TCCTGTCCCA GGTGCTGTCC CTCTGGAGGA GCCCGCTGAT
    GCTCCACTGG CTGCTCAGCC TACTCAGCAT GCGCAGCAGC AGGCAGACGA GGCCATGTGT
    ATCCCTGATG TGGAGATAGC GTGTTGCAGT GTCGGGCCAC CCAAAGCAGT CCCACCAGCT
    GCTGCCAAGG CACCGGAGCT CTGCCCGCTC CCTGTGCTCT CAGATGCTGT TGTTTTGGAG
    CGGCAGCCTG CGGCCCAGAT CCCTCACCTG CCAGAGGCCA GCCAGCCCGG CGTGGTGCAG
    CACTCCTTTG TGAACAAAGT GTTTCCTCCA TCTGTTGCAT CTGAGTCTCT CAATCTGGCA
    GGATCTCAAC TTCAGAGCCC CACTCAGGTG TCCATGGCCG CATCCCAGCA GCACGCGATG
    CTGTCCCAGA CACAGCCTGT CACCTGCGCC AGCGTGGGGA TCAGCCTGCT GCAGCAGCAG
    CAACAGCAGC AGCCGTGCAC CGTGGAGTCT GATGGAGAGG GGCCACCTCG GGTGGACTTT
    GTTGACAACA CAATAAAAAG CCTCGATGAG AAACTGCGCA ACTTACTTTA CCAGGAATAT
    GTCCCAACTT CTTCTGCATC AGCAGGAACT CCAGACGCTT TTGTGCCATT GGAACAGGCA
    GACAGTGAAT TTAACTTGCC ACCACTTACC GAAGATCAAG TTCCCAAGTT GGTGTTGGAT
    TTGAGAGAAC CAGGCCATAA CACCACGGAT AGCTGCCAGG AGGTGAAAGC TGCCGAGGCC
    CAGTTTGTGC CGGCTGTGCC ACCTGAGATC GCACCTTATC CAGCATTGCT TGAAAAGTCT
    GAGGTCGCTG GCATTGGGAC TGAGTCTTTG GAAGCAAGAG GTGGGTCAGC AAGCCAAAGA
    GGATCAGCTG CGAGCATCAC TCCAGCTCCT GTGACTCCAG CAAAGGGCCT CCTCCAGGTT
    GCACCTACAT CATCGAGCAT AGCTAAACAG ATGGAAACTT CTAAAACGAA CGAGAAGGCA
    AAGGCTACAA CTTCATCTAC GCACACAGAT AATGTAATGC AGCTATCGAC AGCAGATGGG
    GTGATAAATA ACCTTCAAAG GGATGACTCT CTAGACTCCG GTTCCTATGG AAAACAGGCT
    GGAACTCATG ATAGCGGTAC ACCAGCCAAA ACTATTGGCA GGTTTTCAGT AAGCAGCACG
    CAAGATGAAT TAACTTTAGC AGCGCCGCAT TGCTTAAGAT TCTCTGCGCC CCCGGACGTC
    TATTTGGATG AGCTCCCTTC CAGCCCCGAC CAGAAGACAG CTGTCCGACG GGTGCAGACG
    GCCTCGTCTG TTGATGTCCT TTGTGACCAG GGCTCGAGTG ATTCTGCCGA GGAGCCCTTC
    CATCGACAGG CTGCTGCGGC TCAGCCATCC CCTCCGAGCA GTAGTGCAGC CTCTGACTTG
    ATTAAAAAAG CAGCAGCTTT TCTGCAAAGG ACTGGAAAAG CAGGCTCCCA AGGCCCCGAT
    TCACCAAACG GGCAGGGAAC AAAGATTCCT ACCATCAACA TAACATCCTT CCACTCGCAG
    TCATCATACA TGAGCAGTGA CAATGACTCT GAATTTGAAG ATGCAGACAT GAAGAAAGAA
    CTGCAGAACT TGAGAGAGAA GCATATGAAA GAAATTGCTG AACTTCAGAC TCAGCAGAAG
    AATGAGATAG AGGCGCTGTA CATTCGGTTA GGGAAGCCCC TCCCTCCCAA CATGGGGTTC
    CTTCACTCAG CCCCGCCAAG CAGCCGTCGA CGAAGAACAA GCAAAAATAA ATTGAAAGGT
    GGAAAACTAT TAAACCCTCT GGTTCAACAG CTCAGGAGTG GAACATCAAG CACAAGTAAT
    GTTTCAGACT CTAAAGACTC ACCCCACAAA GAAACAACTA AAGCTCAGCC TGGATCTCCC
    GAAACAACAG GAGTAACTTC CTCTGTGTCA GCCACATTTG GGAAGTCTGT TCAGACACAG
    CAGCCATGCT CTGTCAAAGC CTCTTTGTCT TCTGACATCT GCTCCGGCTT AGGCCCCGAA
    GGAGGCGATG TGAATGCAAG CTCTGGCCAA GGCTGGACGG TTTTCCACCA AACGTCTGAG
    AGAGTGACCT ATAAATCTAG TAGCAAACCT CGTACCAGAT TCCTCAGTGG ACCTGTGTCT
    TTGTCCATCT GGTCCACCTT GAAACGACTA TGTCTAGGCA AAGATCACAG TAGTAGATCC
    TCTACAAACA GTCTTGTAAC GTGTCCTGAG CCCCTCCCGC AATCAACTCT GCAGGTTCAG
    GCCAACAACA GTAACAACAA GAAGGGAACG TTCACCGATG ATCTTCACAA GCTGGTTGAC
    CAGTGGACAA GCAAAACTGT TGGAGCTGCA CAGGCAAAAC CTTCTTTAAA CCAACTGAAG
    CAGAACCAAA AAAGGCAGGA CATGGAGCCA AAGGCAAATC CCATGCAAAC ACAGAATGAG
    ACATGTGGAG TTAATTCAGT AAGAACAGGA TTGGGGAAAA GGTGCATTGT TCCAGTGTCT
    TGTCCCATGT CAGCTGCGCT AACTCCTGGA GTTTCACCAT CCATGCCAGT GCCTATGCCA
    GGTACTACCC CTTCTGGCGC AGTAACGCCC TGTGTGATGC CTCTTTGTCA GTACGGAGGA
    GTTTTCCCTA CGCCGCTTTA TGGAGTGCAG TGGTCGGGGC CGGCAGTGCG GCCGGGGCTG
    GTAGGAGGGC AGAGCATTGC TCATTTCTCA GCTCTCAGCA AAGCTTGCTT GCAGATGTAC
    CCTGTGCCGC TGCAGCAGCC CGCAGCCGAC CCGCCCGGCC CGCGTGTAAG AATGACATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41682
    Clone ID Related Accession (Same CDS sequence) XM_015293429.1
    Accession Version XM_015293429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGGTAC TACCCCTTCT GGCGCAGTAA CGCCCTGTGT GATGCCTCTT
    TGTCAGTACG GAGGAGTTTT CCCTACGCCG CTTTATGGAG TGCAGTGGTC GGGGCCGGCA
    GTGCGGCCGG GGCTGGTAGG AGGGCAGAGC ATTGCTCATT TCTCAGCTCT CAGCAAAGCT
    TGCTTGCAGA TGTACCCTGT GCCGCTGCAG CAGCCCGCAG CCGACCCGCC CGGCCCGCGT
    GTAAGAATGA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148915.1
    CDS184..6798
    Translation

    Target ORF information:

    RefSeq Version XM_015293429.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GATCCTCTAC AAACAGTCTT GTAACGTGTC CTGAGCCCCT CCCGCAATCA
    ACTCTGCAGG TTCAGGCCAA CAACAGTAAC AACAAGAAGG GAACGTTCAC CGATGATCTT
    CACAAGCTGG TTGACCAGTG GACAAGCAAA ACTGTTGGAG CTGCACAGGC AAAACCTTCT
    TTAAACCAAC TGAAGCAGAA CCAAAAAAGG CAGGACATGG AGCCAAAGGC AAATCCCATG
    CAAACACAGA ATGAGACATG TGGAGTTAAT TCAGTAAGAA CAGGATTGGG GAAAAGGTGC
    ATTGTTCCAG TGTCTTGTCC CATGTCAGCT GCGCTAACTC CTGGAGTTTC ACCATCCATG
    CCAGTGCCTA TGCCAGGTAC TACCCCTTCT GGCGCAGTAA CGCCCTGTGT GATGCCTCTT
    TGTCAGTACG GAGGAGTTTT CCCTACGCCG CTTTATGGAG TGCAGTGGTC GGGGCCGGCA
    GTGCGGCCGG GGCTGGTAGG AGGGCAGAGC ATTGCTCATT TCTCAGCTCT CAGCAAAGCT
    TGCTTGCAGA TGTACCCTGT GCCGCTGCAG CAGCCCGCAG CCGACCCGCC CGGCCCGCGT
    GTAAGAATGA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41683
    Clone ID Related Accession (Same CDS sequence) XM_015293431.1 , XM_015293431.2
    Accession Version XM_015293431.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    ATTCTGAAAA TGCGATCGCC AGCGACTGAC ACGTGGCCCC CGCAGCTACG GTGCCAAGCG
    CCAGGTCCTG CTGCAGCTAA GGAGGAGGAA GAGGAAGATG GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148917.1
    CDS181..6768
    Translation

    Target ORF information:

    RefSeq Version XM_015293431.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293431.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    ATTCTGAAAA TGCGATCGCC AGCGACTGAC ACGTGGCCCC CGCAGCTACG GTGCCAAGCG
    CCAGGTCCTG CTGCAGCTAA GGAGGAGGAA GAGGAAGATG GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41683
    Clone ID Related Accession (Same CDS sequence) XM_015293431.1 , XM_015293431.2
    Accession Version XM_015293431.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    ATTCTGAAAA TGCGATCGCC AGCGACTGAC ACGTGGCCCC CGCAGCTACG GTGCCAAGCG
    CCAGGTCCTG CTGCAGCTAA GGAGGAGGAA GAGGAAGATG GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148917.1
    CDS201..6788
    Translation

    Target ORF information:

    RefSeq Version XM_015293431.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293431.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGATC CTCTACAAAC
    AGTCTTGTAA CGTGTCCTGA GCCCCTCCCG CAATCAACTC TGCAGGTTCA GGCCAACAAC
    AGTAACAACA AGAAGGGAAC GTTCACCGAT GATCTTCACA AGCTGGTTGA CCAGTGGACA
    AGCAAAACTG TTGGAGCTGC ACAGGCAAAA CCTTCTTTAA ACCAACTGAA GCAGAACCAA
    AAAAGGCAGG ACATGGAGCC AAAGGCAAAT CCCATGCAAA CACAGAATGA GACATGTGGA
    ATTCTGAAAA TGCGATCGCC AGCGACTGAC ACGTGGCCCC CGCAGCTACG GTGCCAAGCG
    CCAGGTCCTG CTGCAGCTAA GGAGGAGGAA GAGGAAGATG GGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41684
    Clone ID Related Accession (Same CDS sequence) XM_015293432.2 , XM_015293432.1
    Accession Version XM_015293432.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148918.1
    CDS202..6528
    Translation

    Target ORF information:

    RefSeq Version XM_015293432.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293432.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41684
    Clone ID Related Accession (Same CDS sequence) XM_015293432.2 , XM_015293432.1
    Accession Version XM_015293432.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6327bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product serine/threonine-protein kinase WNK2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148918.1
    CDS183..6509
    Translation

    Target ORF information:

    RefSeq Version XM_015293432.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus WNK lysine deficient protein kinase 2 (WNK2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGAAAGCG GGGGCGATGC CGGAACGGCA CCTCGGCGGG GAGGCGGCGG GCTCTCGGGC 
    GCTGCCCAGC CCGGCGTTCC CCCGGCTGCG GACGGCGGCG TGAGCATGGA TGGGCCCGGC
    GGGAGCGGGG CGGCCGCCGC TGCGGGGCCG GGAGCGCCCG CGCCGCCGCC CCGAGCGCCG
    TGCTCCAGGG ACCCTAAAAT GGTCCATCAG CGGTTCCTGC GGCGGAGCGT GGTGGACTCG
    GACCAGGAGG AGCCCGCGTT CGACCTTCCC GAGTCGGAGC ATCAGAGGAA AATCATCCTG
    CTCCCCAAAA CCAGGAGGAT AATCGCGGAG AGGGCGAAGG CAGGGCACGG GGCGGCGGAG
    AAGGTGTGCC TGGAGACCGA GCCCCCGGGG CCGCTGCAGG CGGGGCCGGC CCCCGGCGGT
    GCGGCGGGGC CCGAGGAGCA GAAGGAGGCC GGCAGGGATG CGGAGAGGAA GGAGGCGTCG
    GACGCGGCCA GGGAGCAGGG CAAGGCCAAG AAAGAGGAGC CCGAGGAGGA GGCTGACATG
    AAGGCGGTCG CCACCTCGCT GGATGGCAGG TTCCTCAAGT TTGATATTGA ACTCGGGAGG
    GGATCCTTCA AAACGGTCTA TAAGGGACTG GACACGGAGA CGTGGGTGGA AGTCGCTTGG
    TGTGAACTTC AGGACAGGAA ATTAACAAAA GTAGAGAGAC AGAGATTTAA AGAGGAAGCA
    GAAATGTTGA AAGGTCTACA GCACCCAAAC ATTGTAAGGT TTTATGACTT CTGGGAATCC
    TGTGTAAAAG GAAAAAGGTG CATCGTGCTG GTTACCGAAC TGATGACCTC TGGAACACTG
    AAGACGTATC TGAAGAGATT TAAAGTGATG AAACCAAAAG TCCTGCGTAG TTGGTGCCGG
    CAAATCCTGA AGGGTCTTCT ATTCTTGCAC ACGAGAACTC CACCAATAAT TCACAGAGAC
    TTAAAATGTG ACAATATTTT TATTACTGGA CCAACTGGAT CTGTGAAAAT AGGAGACTTA
    GGTCTGGCAA CCCTCAAGAG AGCTTCATTT GCCAAAAGTG TAATAGGTAC ACCAGAATTC
    ATGGCCCCAG AAATGTATGA GGAACACTAC GACGAATCCG TTGATGTCTA CGCGTTTGGA
    ATGTGCATGT TGGAAATGGC TACCTCAGAA TACCCTTACT CAGAATGCCA GAATGCTGCT
    CAAATTTACC GGAAAGTAAC CTGTGGTATA AAGCCAGCAA GCTTTGAGAA AGTTACAGAT
    CCTGAAATTA AGGAGATAAT TGGGGAGTGC ATTTGCAAAA ATAAGGAAGA AAGATATGAA
    ATTAAAGATT TATTAAGCCA TGCCTTTTTT GCTGAAGATA CTGGGGTAAG AGTGGAACTT
    GCAGAAGAAG ACCATGGTAG GAAATCCTCT ATTGCCTTAA GACTCTGGGT AGAAGATCCT
    AAGAAACTGA AAGGAAAACC CAAAGATAAT GGAGCCATTG AGTTTACTTT TGATCTGGAG
    AAGGAAACTC CTGATGATGT TGCACAAGAA ATGATTGACT CTGGATTTTT TCATGAAAAC
    GATCTCAAGA TAGTTGCGAA GTCAATACGT GACAGAGTAG CATTAATACT ATGGAGAAGA
    GAGAGAATTT GGCCTAGAAT ACAGTCAGAG GAACGTCGAG ATTCAGAGTG CTTGGAGAAG
    TCGAAGGCAC CGCATCCACA ACAGGTTCAA GTCACATACC TTTCCCACAC CGGTCATCAC
    ATCCTGTCCG AGTCGGAGGA GCCCGAGGCA GACCAGCATC CCTTCCAGCA AAACTTGCCC
    ACCAGCGTCA CATCCGTTGC ATCTGACAGC ACATTCGACA GTGGTCAGGG GTCCACTGTT
    TATTCAGACT CGCAGAGCAG CCAGCAAAGC GTCATCTACT CATCTCTGCC CGACGCCGTG
    CCGCCCACTA TCCAACGGGT GTACAGCCCG CCCCTCAGCG AGAGCCAGGC GCTGCCCCAC
    AGCCTTCCGC AGCTGGGGCA CTACCAGCAG CCCTCAGCAC CTGCCCTGCC CGTGGTTCAG
    GCTCCACCTG CAGTGGTGCC CCAGAGGCCC CAGCACTACC AGGAGCCAGC AATGACGTTC
    CCAGTGGTGC AGCCGACCAC CGTGGCCTCC ATGCAGCTGG GGCAGTCGCA GCCAGTGCTG
    GCCAGCCAGC AGCAACCCAT ATCACAGCAA ACTTCTCTGC AGCAGGTGCT TGCCAGCCAG
    CCGGTTTGCC CCATCCAGCC TGCCCCCCAT CTCCTGCCAC AGTATCAGCC CCAGACCTCT
    CAGGTGGCAG CCAGTAGTAC ACCACTGAAA CCCCTTCAGA TTTCAACTGT GCCACAGCTT
    CCTCCAGTTG CCCCTTCCCA GATTCCTCAG TTTCCCGTCA TTCCTGCAAT TACTCCTCTG
    GCAGGACTTG ACAACCTTCC TCCAAACCTC TCTGATATAC CTGCTGCAAA CGTGCCCCCC
    ATACCTGCTC CTTCTCAGTA TTTTGCTCCA GCTGTGATTT TACCGAGCCT CCCCATGAAC
    CCCACTTTGC CCATGGCACC AAACTCCCCT GCCCTCCCCA TGCAAGCAGT GAACCTTCCT
    CATACAACTG TGGCTTCTCT GGTCTTGCCC TGCCAAACAA TAGTGCCAAA TATGTCGGCT
    GCTACAATAC CGTTGCTTGC TGTGGCTCCG CCGGGAGTGC CAGCGCTGCC GCCGCACCAC
    GCGGTGTCCC AGCTGCCCCA GCAGCAGATG TACCCGACCA CCTTCCAGCA GATAATCCAG
    AGCGAAGCGC CCTCTCCCCA TCACACGCAG AGCACGCAAG CCGTTTCCCA GCCGCAGCAG
    CCTCCACCTC CTCAGTCACT TCAGCCAGGC GTAATTCATC CATCAGAACA GCCGTTGTCC
    GCCCCTGGGG CTGGTAACCA GCAGGTTACT GGACATACTC AGTATGGTTT GGAAACTGGA
    GCCCAGGTGC CGGTACTCCC AGTGCAGCCT TCGCTAGTCA TGTCACCGCC TTTGCAGTTG
    CAACCTGAAC TATTGCCTCC CCGCGTCGCT CCAGAAAGTA TTTCACAGAT TCATGGCAGC
    CTTGCTACAG CTAGTCCACC AGTGCCCATA GATCACTGTC TGCCTCTCCA GCCTTCGGGT
    GTTCTGCAGC TTCAGCCAGA CCTTTCCCAG CCTCTGGCTC AGCAGCTCCC CGCTCCCGGC
    TCAGCCGTGC AGGCAGTGGC AGAGACATCG CAAGAGGAGC AGGCAATACA GGACAAACTT
    CAAACTCTGT CACAGAGCTG TGAAAGCTAC ATGAGTCCAG ATGTCGCTTC AGGTAAAGAG
    ATGAGTGACA GCTTTGAAGG AGCAATAGGA AGTGGAAAAC AAGAAGGAAA GTCTTCAAAG
    AAACATAAGA AATCTACACG TGCTCGTTCA CGCCAGGAGA AAACCAACAG ACCCAAACTG
    ACTATATTAA ATGTTTGTAA CACAGGGGAT AAGATGGTGG AGTGCCAGCT TGAAACACAC
    AATCACAAAA TGGTGACTTT TAAGTTTGAT TTGGATGGTG ATGCGCCTGA GGAAATTGCT
    ACTTACATGG TAGAGAATGA ATTCATATTG CAGAGTGAGA AAGAGACGTT TATTGAACAA
    ATGAAAGATA TTATCGATAA AGCAGAAGAT ATGCTCAGTG AAGATACAGA AGGAGAACGG
    AGTTCTGATC AGGGAACAAG TCCCCAGCAG GATACTGATG GAGCAGAAAT GAGTGAGGAG
    AAGCGGCCAT CTCAAGTGAA GACACCCGTG TATCAACAAA ATGTTTTGCA TACCGGAAAA
    AGGTGGTTTA TTATATGCCC TGTGGTGGAA AACCCGACTA CTGATGCACC AGAATTTTCT
    CCACCAGTTC CTCAGAGTGC ACAACAGTCT GAAGGTCCAG ACCTGGAGCA GTCGGATGAT
    CAGCAAGCAA ACTCTGCGGT ACCGGATACG CCTGGTGTCT TAGCTTGTCA GCAGCTTCCT
    CTACAAGCAA GCGTCTGTGA CAGCCCCATT GGGGCCTCTC CATCTTTGGC ACAAATTCCT
    GCTGCAGCAT CTTCAGCTGG CTTGCCAAGC CAGGCAGAGC TCTCAGTTCC AGCACCGCCA
    GGACCTGCTC CAAACATCCT GGAGCAGGCA GCCGCTAACG GAGGCCAGCC GGAATGTTCT
    CTACTGAAAG CAGCATTGCC CGCCCCACCT GGCACTCCTG TCCCAGGTGC TGTCCCTCTG
    GAGGAGCCCG CTGATGCTCC ACTGGCTGCT CAGCCTACTC AGCATGCGCA GCAGCAGGCA
    GACGAGGCCA TGTGTATCCC TGATGTGGAG ATAGCGTGTT GCAGTGTCGG GCCACCCAAA
    GCAGTCCCAC CAGCTGCTGC CAAGGCACCG GAGCTCTGCC CGCTCCCTGT GCTCTCAGAT
    GCTGTTGTTT TGGAGCGGCA GCCTGCGGCC CAGATCCCTC ACCTGCCAGA GGCCAGCCAG
    CCCGGCGTGG TGCAGCACTC CTTTGTGAAC AAAGTGTTTC CTCCATCTGT TGCATCTGAG
    TCTCTCAATC TGGCAGGATC TCAACTTCAG AGCCCCACTC AGGTGTCCAT GGCCGCATCC
    CAGCAGCACG CGATGCTGTC CCAGACACAG CCTGTCACCT GCGCCAGCGT GGGGATCAGC
    CTGCTGCAGC AGCAGCAACA GCAGCAGCCG TGCACCGTGG AGTCTGATGG AGAGGGGCCA
    CCTCGGGTGG ACTTTGTTGA CAACACAATA AAAAGCCTCG ATGAGAAACT GCGCAACTTA
    CTTTACCAGG AATATGTCCC AACTTCTTCT GCATCAGCAG GAACTCCAGA CGCTTTTGTG
    CCATTGGAAC AGGCAGACAG TGAATTTAAC TTGCCACCAC TTACCGAAGA TCAAGTTCCC
    AAGTTGGTGT TGGATTTGAG AGAACCAGGC CATAACACCA CGGATAGCTG CCAGGAGGTG
    AAAGCTGCCG AGGCCCAGTT TGTGCCGGCT GTGCCACCTG AGATCGCACC TTATCCAGCA
    TTGCTTGAAA AGTCTGAGGT CGCTGGCATT GGGACTGAGT CTTTGGAAGC AAGAGGTGGG
    TCAGCAAGCC AAAGAGGATC AGCTGCGAGC ATCACTCCAG CTCCTGTGAC TCCAGCAAAG
    GGCCTCCTCC AGGTTGCACC TACATCATCG AGCATAGCTA AACAGATGGA AACTTCTAAA
    ACGAACGAGA AGGCAAAGGC TACAACTTCA TCTACGCACA CAGATAATGT AATGCAGCTA
    TCGACAGCAG ATGGGGTGAT AAATAACCTT CAAAGGGATG ACTCTCTAGA CTCCGGTTCC
    TATGGAAAAC AGGCTGGAAC TCATGATAGC GGTACACCAG CCAAAACTAT TGGCAGGTTT
    TCAGTAAGCA GCACGCAAGA TGAATTAACT TTAGCAGCGC CGCATTGCTT AAGATTCTCT
    GCGCCCCCGG ACGTCTATTT GGATGAGCTC CCTTCCAGCC CCGACCAGAA GACAGCTGTC
    CGACGGGTGC AGACGGCCTC GTCTGTTGAT GTCCTTTGTG ACCAGGGCTC GAGTGATTCT
    GCCGAGGAGC CCTTCCATCG ACAGGCTGCT GCGGCTCAGC CATCCCCTCC GAGCAGTAGT
    GCAGCCTCTG ACTTGATTAA AAAAGCAGCA GCTTTTCTGC AAAGGACTGG AAAAGCAGGC
    TCCCAAGGCC CCGATTCACC AAACGGGCAG GGAACAAAGA TTCCTACCAT CAACATAACA
    TCCTTCCACT CGCAGTCATC ATACATGAGC AGTGACAATG ACTCTGAATT TGAAGATGCA
    GACATGAAGA AAGAACTGCA GAACTTGAGA GAGAAGCATA TGAAAGAAAT TGCTGAACTT
    CAGACTCAGC AGAAGAATGA GATAGAGGCG CTGTACATTC GGTTAGGGAA GCCCCTCCCT
    CCCAACATGG GGTTCCTTCA CTCAGCCCCG CCAAGCAGCC GTCGACGAAG AACAAGCAAA
    AATAAATTGA AAGGTGGAAA ACTATTAAAC CCTCTGGTTC AACAGCTCAG GAGTGGAACA
    TCAAGCACAA GTAATGTTTC AGACTCTAAA GACTCACCCC ACAAAGAAAC AACTAAAGCT
    CAGCCTGGAT CTCCCGAAAC AACAGGAGTA ACTTCCTCTG TGTCAGCCAC ATTTGGGAAG
    TCTGTTCAGA CACAGCAGCC ATGCTCTGTC AAAGCCTCTT TGTCTTCTGA CATCTGCTCC
    GGCTTAGGCC CCGAAGGAGG CGATGTGAAT GCAAGCTCTG GCCAAGGCTG GACGGTTTTC
    CACCAAACGT CTGAGAGAGT GACCTATAAA TCTAGTAGCA AACCTCGTAC CAGATTCCTC
    AGTGGACCTG TGTCTTTGTC CATCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.