IARS cDNA ORF clone, Gallus gallus(chicken)

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa41720 XM_015293480.2
Latest version!
Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa41720 XM_015293481.2
Latest version!
Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa14086 XM_414300.5
Latest version!
Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OGa14086 XM_414300.4 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa41720 XM_015293480.1 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa41720 XM_015293481.1 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa41720
    Clone ID Related Accession (Same CDS sequence) XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
    Accession Version XM_015293480.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148966.1
    CDS158..3955
    Translation

    Target ORF information:

    RefSeq Version XM_015293480.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293480.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41720
    Clone ID Related Accession (Same CDS sequence) XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
    Accession Version XM_015293480.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148966.1
    CDS182..3979
    Translation

    Target ORF information:

    RefSeq Version XM_015293480.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41720
    Clone ID Related Accession (Same CDS sequence) XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
    Accession Version XM_015293481.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148967.1
    CDS113..3910
    Translation

    Target ORF information:

    RefSeq Version XM_015293481.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa41720
    Clone ID Related Accession (Same CDS sequence) XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
    Accession Version XM_015293481.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015148967.1
    CDS178..3975
    Translation

    Target ORF information:

    RefSeq Version XM_015293481.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015293481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
    CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG
    TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT
    GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC
    AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA
    GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC
    AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC
    TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG
    TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT
    TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT
    GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT
    GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA
    GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG
    TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT
    GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA
    GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT
    GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT
    ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT
    GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA
    CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC
    TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG
    CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG
    AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC
    CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT
    ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG
    GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC
    CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT
    CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA
    AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA
    ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT
    GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC
    AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT
    CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT
    TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC
    ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA
    GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC
    AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC
    AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG
    ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA
    AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA
    AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA
    ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT
    CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG
    ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG
    GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG
    AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG
    CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT
    CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG
    TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA
    AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT
    GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT
    GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG
    CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT
    CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG
    TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA
    GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA
    CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG
    TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG
    TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA
    AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT
    TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC
    ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC
    ACCACAGCTG AATGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14086
    Clone ID Related Accession (Same CDS sequence) XM_414300.4 , XM_414300.5
    Accession Version XM_414300.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_414300.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
    GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG
    CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT
    GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT
    AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA
    TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG
    CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG
    ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT
    GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG
    CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT
    TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT
    TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC
    ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT
    GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA
    GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG
    AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC
    TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA
    GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA
    GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC
    TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG
    GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT
    ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG
    AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT
    TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA
    ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG
    GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT
    CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT
    GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC
    AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC
    CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC
    AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG
    AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG
    TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA
    GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT
    CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC
    ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC
    ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA
    GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG
    GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT
    TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT
    CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC
    CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT
    TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA
    AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC
    AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG
    AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG
    CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG
    CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG
    GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA
    CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT
    GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA
    GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT
    ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT
    TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG
    GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC
    TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG
    AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT
    TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA
    ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT
    CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA
    AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT
    CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA
    AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC
    TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_414300.3
    CDS37..3882
    Translation

    Target ORF information:

    RefSeq Version XM_414300.4
    Organism Gallus gallus(chicken)
    Definition Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_414300.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
    GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG
    CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT
    GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT
    AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA
    TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG
    CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG
    ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT
    GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG
    CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT
    TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT
    TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC
    ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT
    GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA
    GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG
    AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC
    TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA
    GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA
    GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC
    TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG
    GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT
    ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG
    AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT
    TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA
    ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG
    GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT
    CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT
    GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC
    AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC
    CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC
    AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG
    AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG
    TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA
    GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT
    CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC
    ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC
    ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA
    GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG
    GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT
    TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT
    CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC
    CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT
    TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA
    AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC
    AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG
    AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG
    CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG
    CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG
    GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA
    CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT
    GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA
    GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT
    ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT
    TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG
    GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC
    TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG
    AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT
    TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA
    ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT
    CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA
    AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT
    CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA
    AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC
    TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa14086
    Clone ID Related Accession (Same CDS sequence) XM_414300.4 , XM_414300.5
    Accession Version XM_414300.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_414300.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
    GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG
    CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT
    GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT
    AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA
    TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG
    CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG
    ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT
    GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG
    CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT
    TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT
    TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC
    ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT
    GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA
    GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG
    AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC
    TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA
    GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA
    GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC
    TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG
    GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT
    ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG
    AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT
    TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA
    ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG
    GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT
    CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT
    GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC
    AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC
    CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC
    AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG
    AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG
    TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA
    GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT
    CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC
    ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC
    ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA
    GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG
    GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT
    TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT
    CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC
    CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT
    TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA
    AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC
    AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG
    AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG
    CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG
    CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG
    GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA
    CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT
    GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA
    GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT
    ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT
    TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG
    GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC
    TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG
    AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT
    TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA
    ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT
    CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA
    AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT
    CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA
    AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC
    TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_414300.3
    CDS29..3874
    Translation

    Target ORF information:

    RefSeq Version XM_414300.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_414300.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
    GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG
    CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT
    GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT
    AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA
    TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG
    CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG
    ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT
    GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG
    CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT
    TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT
    TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC
    ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT
    GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA
    GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG
    AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC
    TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA
    GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA
    GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC
    TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG
    GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT
    ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG
    AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT
    TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA
    ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG
    GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT
    CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT
    GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC
    AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC
    CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC
    AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG
    AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG
    TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA
    GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT
    CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC
    ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC
    ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA
    GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG
    GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT
    TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT
    CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC
    CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT
    TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA
    AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC
    AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG
    AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG
    CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG
    CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG
    GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA
    CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT
    GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA
    GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT
    ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT
    TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG
    GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC
    TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG
    AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT
    TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA
    ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT
    CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA
    AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT
    CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA
    AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC
    TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA
    TGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.