SLC12A1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A1
Entrez Gene ID	415433
Full Name	solute carrier family 12 member 1
General protein information	Preferred Names solute carrier family 12 member 1 Names solute carrier family 12 member 1 solute carrier family 12 (sodium/potassium/chloride transporter), member 1 solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_413814.5	XP_413814.4	solute carrier family 12 member 1 isoform X1
XM_413814.6	XP_413814.5	solute carrier family 12 member 1 isoform X1
XM_025154033.1	XP_025009801.1	solute carrier family 12 member 1 isoform X3
XM_004943799.2	XP_004943856.2	solute carrier family 12 member 1 isoform X2
XM_004943799.3	XP_004943856.3	solute carrier family 12 member 1 isoform X2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Gene Ontology

The following SLC12A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa143531	XM_413814.6 Latest version!	Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa143532	XM_004943799.3 Latest version!	Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa143533	XM_025154033.1 Latest version!	Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa40506	XM_413814.5 Latest version!	Gallus gallus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa40507	XM_004943799.2 Latest version!	Gallus gallus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A1 ( XM_413814.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_413814.5 Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa143531
Clone ID Related Accession (Same CDS sequence)	XM_413814.6
Accession Version	XM_413814.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_413814.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAG 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATATGAC AATTTTCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA ATCATAGTTA TTGCTCTGAG CGTAGTAGTA 
ACAACTTTAA CAGGTATCTC CATGTCTGCC ATTTGCACTA ATGGAGTAGT AAGAGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_413814.5
CDS	2345..5638
Translation	MSMNNSPNVFLDPNANRFQVNIINESHNSNEASQEDVESEPPHYEETSFTEEAHNRLRISYRPGKRELYDNFLQNGEKPEASLHPCDTHNKMYYLQTFGHNTMDPVPKIDYYRNTGSVSGNKLNRPSLLEIHEQLAKNIAVSTGSVERVANGESTAGEETAASKEEEKKTGFVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGIIVIALSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLKESDTIMVDESNDIRIIGTITVVCLLGISVAGMEWEAKAQVILLIVLLIAIANFFIGTVIPTNNEKKARGFFNYQASIFAENFGPDFRSGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMLAILITTVAYIGVAVCAASCVVRDATGNVNDTVIPGMSCNGSSACNLGYDFSRCRSQPCDYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNVYKGLHFFAKGYGKNNEPIRGYVLTFVIAMAFILIAQLNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFRYYNMWISLFGALLCCGVMFVINWWAALITYVIELFLYIYVTYKKPEVNWGSSTQALCYIKALDSALALTTVEDHVKNFRPQCIVLTGAPMIRPALLDITHSFTKNNGLCICCEVYTGPRKLCVKEMNSGMAKKQAWLTKNKRKAFYAAVAADSFRDGVKSLLQASGLGRMKPNTLVIGFKKDWRNAAVTQVENYVGVIHDAFDFELGTIIVRIGQGFDISQVLQVQEELEKLEQERLALEATIKENEFEEEKSGICGFFRKARKLNITKHEAKNESTINTVQSMHVGEFNQKLLEASTQFKKKQGKGTIDIWWLFDDGGLTILIPYILTIRKKWKNCKLRIFTGGKVNRIEEEKLVMASLLSKFRIKFADINIICDINMKPNKESWKFFEEMIEPYRLRESCKDITTAEKLKRETPWKITDAELEAFKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGVVSDYLYMAWLEILSKNLPPILMVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_413814.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_413814.6

ORF Insert Sequence:

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAG 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATATGAC AATTTTCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA ATCATAGTTA TTGCTCTGAG CGTAGTAGTA 
ACAACTTTAA CAGGTATCTC CATGTCTGCC ATTTGCACTA ATGGAGTAGT AAGAGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_004943799.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943856.3 Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa143532
Clone ID Related Accession (Same CDS sequence)	XM_004943799.3
Accession Version	XM_004943799.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943799.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAG 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATATGAC AATTTTCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA GTAATCATAA TTCTCCTCGC CACGATGGTA 
ACCTCAATTA CTGGGTTGTC AACTTCTGCA ATAGCAACTA ATGGGTTTGT CCGTGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943856.3
CDS	2346..5639
Translation	MSMNNSPNVFLDPNANRFQVNIINESHNSNEASQEDVESEPPHYEETSFTEEAHNRLRISYRPGKRELYDNFLQNGEKPEASLHPCDTHNKMYYLQTFGHNTMDPVPKIDYYRNTGSVSGNKLNRPSLLEIHEQLAKNIAVSTGSVERVANGESTAGEETAASKEEEKKTGFVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVIIILLATMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLKESDTIMVDESNDIRIIGTITVVCLLGISVAGMEWEAKAQVILLIVLLIAIANFFIGTVIPTNNEKKARGFFNYQASIFAENFGPDFRSGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMLAILITTVAYIGVAVCAASCVVRDATGNVNDTVIPGMSCNGSSACNLGYDFSRCRSQPCDYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNVYKGLHFFAKGYGKNNEPIRGYVLTFVIAMAFILIAQLNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFRYYNMWISLFGALLCCGVMFVINWWAALITYVIELFLYIYVTYKKPEVNWGSSTQALCYIKALDSALALTTVEDHVKNFRPQCIVLTGAPMIRPALLDITHSFTKNNGLCICCEVYTGPRKLCVKEMNSGMAKKQAWLTKNKRKAFYAAVAADSFRDGVKSLLQASGLGRMKPNTLVIGFKKDWRNAAVTQVENYVGVIHDAFDFELGTIIVRIGQGFDISQVLQVQEELEKLEQERLALEATIKENEFEEEKSGICGFFRKARKLNITKHEAKNESTINTVQSMHVGEFNQKLLEASTQFKKKQGKGTIDIWWLFDDGGLTILIPYILTIRKKWKNCKLRIFTGGKVNRIEEEKLVMASLLSKFRIKFADINIICDINMKPNKESWKFFEEMIEPYRLRESCKDITTAEKLKRETPWKITDAELEAFKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGVVSDYLYMAWLEILSKNLPPILMVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_004943799.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943799.3

ORF Insert Sequence:

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAG 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATATGAC AATTTTCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA GTAATCATAA TTCTCCTCGC CACGATGGTA 
ACCTCAATTA CTGGGTTGTC AACTTCTGCA ATAGCAACTA ATGGGTTTGT CCGTGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_025154033.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025009801.1 Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa143533
Clone ID Related Accession (Same CDS sequence)	XM_025154033.1
Accession Version	XM_025154033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006097.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAG 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATATGAC AATTTTCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA ATCATAGTTA TTGCTCTGAG CGTAGTAGTA 
ACAACTTTAA CAGGTATCTC CATGTCTGCC ATTTGCACTA ATGGAGTAGT AAGAGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGC TCAAGTTATT CTTCTAATTG TTCTCCTTAT TGCCATTGCA 
AACTTCTTTA TTGGGACAGT TATTCCTACC AACAACGAAA AGAAGGCAAG AGGCTTTTTT 
AATTACCAAG CATCAATATT TGCAGAAAAC TTTGGACCAG ATTTCAGAAG TGGAGAAGGA 
TTTTTCTCAG TGTTTGCCAT CTTCTTCCCA GCTGCCACTG GCATTCTCGC GGGAGCCAAC 
ATCTCAGGAG ATCTGAAAGA TCCGCAGGGT GCGATACCCA AAGGAACAAT GCTGGCCATT 
CTGATAACAA CAGTTGCTTA CATAGGCGTT GCAGTATGTG CAGCCTCCTG TGTTGTACGA 
GATGCTACTG GAAACGTCAA TGATACAGTT ATACCTGGGA TGAGCTGCAA TGGATCATCT 
GCCTGCAACC TAGGCTATGA TTTTTCCAGA TGCAGAAGTC AGCCATGCGA TTACGGGCTG 
ATGAATAATT TTCAGGTGAT GAGTATGGTG TCTGGCTTTG GTCCTCTCAT CACAGCTGGC 
ATCTTTTCTG CTACACTGTC ATCAGCTCTG GCATCTCTCG TCAGCGCACC CAAAGTTTTT 
CAGGCTCTTT GCAAGGACAA TGTCTACAAA GGACTCCACT TCTTTGCCAA AGGATACGGA 
AAAAACAACG AGCCAATCAG AGGATATGTA CTCACTTTTG TGATTGCTAT GGCGTTCATT 
TTGATTGCTC AGTTGAACAC CATTGCTCCC ATCATCTCCA ATTTCTTCCT GGCTTCATAT 
GCTTTGATTA ATTTCTCCTG CTTTCATGCC TCATACGCAA AATCTCCAGG TTGGAGACCT 
GCATTCAGAT ATTATAACAT GTGGATATCG CTTTTTGGAG CCCTGCTGTG CTGTGGCGTT 
ATGTTTGTCA TCAACTGGTG GGCAGCTCTC ATCACTTATG TCATTGAACT CTTTCTATAT 
ATTTATGTAA CATATAAGAA ACCAGAAGTA AATTGGGGCT CTTCTACACA GGCTCTTTGC 
TATATTAAGG CCTTAGACAG TGCGCTGGCA TTAACTACAG TGGAAGATCA TGTGAAAAAC 
TTCAGACCCC AGTGCATAGT ATTAACAGGA GCTCCTATGA TCAGGCCTGC TCTTCTAGAC 
ATCACACACA GTTTCACAAA GAACAATGGG CTGTGCATCT GCTGTGAAGT GTATACGGGA 
CCACGCAAGC TGTGTGTTAA GGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ACAAAGAACA AAAGAAAAGC CTTTTATGCA GCAGTGGCAG CTGACAGCTT CAGAGATGGA 
GTCAAAAGTC TCCTTCAAGC CTCAGGATTG GGTAGAATGA AACCAAATAC CTTGGTGATT 
GGATTTAAGA AGGACTGGAG AAATGCTGCT GTCACACAAG TTGAAAACTA TGTGGGTGTT 
ATACATGATG CCTTTGATTT TGAACTTGGC ACGATTATTG TCAGAATCGG CCAAGGATTT 
GATATATCTC AGGTCCTCCA GGTTCAAGAG GAATTAGAGA AGCTGGAGCA GGAGAGATTG 
GCACTGGAAG CTACAATTAA AGAAAATGAA TTTGAAGAAG AAAAAAGTGG CATCTGCGGA 
TTCTTCAGAA AAGCCAGAAA GCTCAATATC ACAAAACATG AAGCAAAAAA TGAGAGCACC 
ATTAACACAG TGCAGTCAAT GCATGTGGGA GAATTCAATC AGAAGCTTCT AGAAGCGAGC 
ACTCAATTTA AAAAGAAACA AGGAAAAGGC ACAATTGACA TTTGGTGGCT GTTTGATGAT 
GGAGGTCTAA CAATCCTAAT TCCTTACATT CTAACTATCA GGAAGAAGTG GAAAAATTGC 
AAATTAAGGA TCTTCACAGG AGGAAAAGTC AATAGGATTG AAGAAGAAAA ACTAGTGATG 
GCTTCACTTC TAAGCAAATT TAGAATAAAA TTTGCTGACA TTAATATCAT TTGTGATATC 
AATATGAAAC CCAACAAAGA GAGTTGGAAA TTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCGTGAAA GCTGCAAAGA TATAACAACT GCTGAGAAAT TAAAGCGGGA GACTCCATGG 
AAAATTACAG ATGCAGAGCT GGAAGCATTC AAAGAAAAGA GTTATCGCCA AGTTCGTTTG 
AATGAACTCT TACAAGAGCA TTCGAGGGCA GCTAATCTCA TTGTCCTGAG TCTCCCAGTG 
GCAAGAAAAG GAGTAGTGTC TGATTACCTG TATATGGCTT GGCTAGAAAT TCTCTCAAAG 
AACCTTCCTC CAATCTTAAT GGTCAGGGGA AACCACAAGA ACGTACTGAC TTTCTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025009801.1
CDS	2345..5527
Translation	MSMNNSPNVFLDPNANRFQVNIINESHNSNEASQEDVESEPPHYEETSFTEEAHNRLRISYRPGKRELYDNFLQNGEKPEASLHPCDTHNKMYYLQTFGHNTMDPVPKIDYYRNTGSVSGNKLNRPSLLEIHEQLAKNIAVSTGSVERVANGESTAGEETAASKEEEKKTGFVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGIIVIALSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLKAQVILLIVLLIAIANFFIGTVIPTNNEKKARGFFNYQASIFAENFGPDFRSGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMLAILITTVAYIGVAVCAASCVVRDATGNVNDTVIPGMSCNGSSACNLGYDFSRCRSQPCDYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNVYKGLHFFAKGYGKNNEPIRGYVLTFVIAMAFILIAQLNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFRYYNMWISLFGALLCCGVMFVINWWAALITYVIELFLYIYVTYKKPEVNWGSSTQALCYIKALDSALALTTVEDHVKNFRPQCIVLTGAPMIRPALLDITHSFTKNNGLCICCEVYTGPRKLCVKEMNSGMAKKQAWLTKNKRKAFYAAVAADSFRDGVKSLLQASGLGRMKPNTLVIGFKKDWRNAAVTQVENYVGVIHDAFDFELGTIIVRIGQGFDISQVLQVQEELEKLEQERLALEATIKENEFEEEKSGICGFFRKARKLNITKHEAKNESTINTVQSMHVGEFNQKLLEASTQFKKKQGKGTIDIWWLFDDGGLTILIPYILTIRKKWKNCKLRIFTGGKVNRIEEEKLVMASLLSKFRIKFADINIICDINMKPNKESWKFFEEMIEPYRLRESCKDITTAEKLKRETPWKITDAELEAFKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGVVSDYLYMAWLEILSKNLPPILMVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_025154033.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus solute carrier family 12 member 1 (SLC12A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154033.1

ORF Insert Sequence:

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAG 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATATGAC AATTTTCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA ATCATAGTTA TTGCTCTGAG CGTAGTAGTA 
ACAACTTTAA CAGGTATCTC CATGTCTGCC ATTTGCACTA ATGGAGTAGT AAGAGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGC TCAAGTTATT CTTCTAATTG TTCTCCTTAT TGCCATTGCA 
AACTTCTTTA TTGGGACAGT TATTCCTACC AACAACGAAA AGAAGGCAAG AGGCTTTTTT 
AATTACCAAG CATCAATATT TGCAGAAAAC TTTGGACCAG ATTTCAGAAG TGGAGAAGGA 
TTTTTCTCAG TGTTTGCCAT CTTCTTCCCA GCTGCCACTG GCATTCTCGC GGGAGCCAAC 
ATCTCAGGAG ATCTGAAAGA TCCGCAGGGT GCGATACCCA AAGGAACAAT GCTGGCCATT 
CTGATAACAA CAGTTGCTTA CATAGGCGTT GCAGTATGTG CAGCCTCCTG TGTTGTACGA 
GATGCTACTG GAAACGTCAA TGATACAGTT ATACCTGGGA TGAGCTGCAA TGGATCATCT 
GCCTGCAACC TAGGCTATGA TTTTTCCAGA TGCAGAAGTC AGCCATGCGA TTACGGGCTG 
ATGAATAATT TTCAGGTGAT GAGTATGGTG TCTGGCTTTG GTCCTCTCAT CACAGCTGGC 
ATCTTTTCTG CTACACTGTC ATCAGCTCTG GCATCTCTCG TCAGCGCACC CAAAGTTTTT 
CAGGCTCTTT GCAAGGACAA TGTCTACAAA GGACTCCACT TCTTTGCCAA AGGATACGGA 
AAAAACAACG AGCCAATCAG AGGATATGTA CTCACTTTTG TGATTGCTAT GGCGTTCATT 
TTGATTGCTC AGTTGAACAC CATTGCTCCC ATCATCTCCA ATTTCTTCCT GGCTTCATAT 
GCTTTGATTA ATTTCTCCTG CTTTCATGCC TCATACGCAA AATCTCCAGG TTGGAGACCT 
GCATTCAGAT ATTATAACAT GTGGATATCG CTTTTTGGAG CCCTGCTGTG CTGTGGCGTT 
ATGTTTGTCA TCAACTGGTG GGCAGCTCTC ATCACTTATG TCATTGAACT CTTTCTATAT 
ATTTATGTAA CATATAAGAA ACCAGAAGTA AATTGGGGCT CTTCTACACA GGCTCTTTGC 
TATATTAAGG CCTTAGACAG TGCGCTGGCA TTAACTACAG TGGAAGATCA TGTGAAAAAC 
TTCAGACCCC AGTGCATAGT ATTAACAGGA GCTCCTATGA TCAGGCCTGC TCTTCTAGAC 
ATCACACACA GTTTCACAAA GAACAATGGG CTGTGCATCT GCTGTGAAGT GTATACGGGA 
CCACGCAAGC TGTGTGTTAA GGAGATGAAC AGTGGCATGG CAAAAAAGCA GGCCTGGCTT 
ACAAAGAACA AAAGAAAAGC CTTTTATGCA GCAGTGGCAG CTGACAGCTT CAGAGATGGA 
GTCAAAAGTC TCCTTCAAGC CTCAGGATTG GGTAGAATGA AACCAAATAC CTTGGTGATT 
GGATTTAAGA AGGACTGGAG AAATGCTGCT GTCACACAAG TTGAAAACTA TGTGGGTGTT 
ATACATGATG CCTTTGATTT TGAACTTGGC ACGATTATTG TCAGAATCGG CCAAGGATTT 
GATATATCTC AGGTCCTCCA GGTTCAAGAG GAATTAGAGA AGCTGGAGCA GGAGAGATTG 
GCACTGGAAG CTACAATTAA AGAAAATGAA TTTGAAGAAG AAAAAAGTGG CATCTGCGGA 
TTCTTCAGAA AAGCCAGAAA GCTCAATATC ACAAAACATG AAGCAAAAAA TGAGAGCACC 
ATTAACACAG TGCAGTCAAT GCATGTGGGA GAATTCAATC AGAAGCTTCT AGAAGCGAGC 
ACTCAATTTA AAAAGAAACA AGGAAAAGGC ACAATTGACA TTTGGTGGCT GTTTGATGAT 
GGAGGTCTAA CAATCCTAAT TCCTTACATT CTAACTATCA GGAAGAAGTG GAAAAATTGC 
AAATTAAGGA TCTTCACAGG AGGAAAAGTC AATAGGATTG AAGAAGAAAA ACTAGTGATG 
GCTTCACTTC TAAGCAAATT TAGAATAAAA TTTGCTGACA TTAATATCAT TTGTGATATC 
AATATGAAAC CCAACAAAGA GAGTTGGAAA TTCTTTGAAG AGATGATTGA ACCATATCGT 
CTCCGTGAAA GCTGCAAAGA TATAACAACT GCTGAGAAAT TAAAGCGGGA GACTCCATGG 
AAAATTACAG ATGCAGAGCT GGAAGCATTC AAAGAAAAGA GTTATCGCCA AGTTCGTTTG 
AATGAACTCT TACAAGAGCA TTCGAGGGCA GCTAATCTCA TTGTCCTGAG TCTCCCAGTG 
GCAAGAAAAG GAGTAGTGTC TGATTACCTG TATATGGCTT GGCTAGAAAT TCTCTCAAAG 
AACCTTCCTC CAATCTTAAT GGTCAGGGGA AACCACAAGA ACGTACTGAC TTTCTATTCT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_413814.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_413814.4 Gallus gallus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa40506
Clone ID Related Accession (Same CDS sequence)	XM_413814.5
Accession Version	XM_413814.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_413814.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAC 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATACGAC AATTTCCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA ATCATAGTTA TTGCTCTGAG CGTAGTAGTA 
ACAACTTTAA CAGGTATCTC CATGTCTGCC ATTTGCACTA ATGGAGTAGT AAGAGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_413814.4
CDS	2313..5606
Translation	MSMNNSPNVFLDPNANRFQVNIINESHNSNEASQEDVESDPPHYEETSFTEEAHNRLRISYRPGKRELYDNFLQNGEKPEASLHPCDTHNKMYYLQTFGHNTMDPVPKIDYYRNTGSVSGNKLNRPSLLEIHEQLAKNIAVSTGSVERVANGESTAGEETAASKEEEKKTGFVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGIIVIALSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLKESDTIMVDESNDIRIIGTITVVCLLGISVAGMEWEAKAQVILLIVLLIAIANFFIGTVIPTNNEKKARGFFNYQASIFAENFGPDFRSGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMLAILITTVAYIGVAVCAASCVVRDATGNVNDTVIPGMSCNGSSACNLGYDFSRCRSQPCDYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNVYKGLHFFAKGYGKNNEPIRGYVLTFVIAMAFILIAQLNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFRYYNMWISLFGALLCCGVMFVINWWAALITYVIELFLYIYVTYKKPEVNWGSSTQALCYIKALDSALALTTVEDHVKNFRPQCIVLTGAPMIRPALLDITHSFTKNNGLCICCEVYTGPRKLCVKEMNSGMAKKQAWLTKNKRKAFYAAVAADSFRDGVKSLLQASGLGRMKPNTLVIGFKKDWRNAAVTQVENYVGVIHDAFDFELGTIIVRIGQGFDISQVLQVQEELEKLEQERLALEATIKENEFEEEKSGICGFFRKARKLNITKHEAKNESTINTVQSMHVGEFNQKLLEASTQFKKKQGKGTIDIWWLFDDGGLTILIPYILTIRKKWKNCKLRIFTGGKVNRIEEEKLVMASLLSKFRIKFADINIICDINMKPNKESWKFFEEMIEPYRLRESCKDITTAEKLKRETPWKITDAELEAFKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGVVSDYLYMAWLEILSKNLPPILMVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_413814.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_413814.5

ORF Insert Sequence:

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAC 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATACGAC AATTTCCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA ATCATAGTTA TTGCTCTGAG CGTAGTAGTA 
ACAACTTTAA CAGGTATCTC CATGTCTGCC ATTTGCACTA ATGGAGTAGT AAGAGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_004943799.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004943856.2 Gallus gallus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

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CloneID	OGa40507
Clone ID Related Accession (Same CDS sequence)	XM_004943799.2
Accession Version	XM_004943799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455923.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAC 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATACGAC AATTTCCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA GTAATCATAA TTCTCCTCGC CACGATGGTA 
ACCTCAATTA CTGGGTTGTC AACTTCTGCA ATAGCAACTA ATGGGTTTGT CCGTGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004943856.2
CDS	2313..5606
Translation	MSMNNSPNVFLDPNANRFQVNIINESHNSNEASQEDVESDPPHYEETSFTEEAHNRLRISYRPGKRELYDNFLQNGEKPEASLHPCDTHNKMYYLQTFGHNTMDPVPKIDYYRNTGSVSGNKLNRPSLLEIHEQLAKNIAVSTGSVERVANGESTAGEETAASKEEEKKTGFVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLGVIIILLATMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVELLKESDTIMVDESNDIRIIGTITVVCLLGISVAGMEWEAKAQVILLIVLLIAIANFFIGTVIPTNNEKKARGFFNYQASIFAENFGPDFRSGEGFFSVFAIFFPAATGILAGANISGDLKDPQGAIPKGTMLAILITTVAYIGVAVCAASCVVRDATGNVNDTVIPGMSCNGSSACNLGYDFSRCRSQPCDYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNVYKGLHFFAKGYGKNNEPIRGYVLTFVIAMAFILIAQLNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAFRYYNMWISLFGALLCCGVMFVINWWAALITYVIELFLYIYVTYKKPEVNWGSSTQALCYIKALDSALALTTVEDHVKNFRPQCIVLTGAPMIRPALLDITHSFTKNNGLCICCEVYTGPRKLCVKEMNSGMAKKQAWLTKNKRKAFYAAVAADSFRDGVKSLLQASGLGRMKPNTLVIGFKKDWRNAAVTQVENYVGVIHDAFDFELGTIIVRIGQGFDISQVLQVQEELEKLEQERLALEATIKENEFEEEKSGICGFFRKARKLNITKHEAKNESTINTVQSMHVGEFNQKLLEASTQFKKKQGKGTIDIWWLFDDGGLTILIPYILTIRKKWKNCKLRIFTGGKVNRIEEEKLVMASLLSKFRIKFADINIICDINMKPNKESWKFFEEMIEPYRLRESCKDITTAEKLKRETPWKITDAELEAFKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGVVSDYLYMAWLEILSKNLPPILMVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_004943799.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004943799.2

ORF Insert Sequence:

ATGTCAATGA ATAATTCTCC AAATGTATTC TTGGATCCAA ATGCAAATCG GTTTCAAGTT 
AACATAATTA ATGAAAGCCA CAACAGCAAT GAAGCATCCC AAGAGGACGT GGAATCTGAC 
CCACCACATT ATGAAGAAAC ATCTTTTACT GAGGAAGCAC ACAACAGACT GAGAATCAGT 
TACAGACCAG GAAAAAGAGA ATTATACGAC AATTTCCTTC AAAATGGGGA GAAACCAGAG 
GCCAGTTTAC ATCCCTGTGA TACTCATAAT AAAATGTATT ATCTACAAAC TTTTGGCCAT 
AACACCATGG ATCCAGTTCC TAAAATTGAT TATTATCGAA ACACTGGCAG CGTCAGTGGG 
AATAAGCTCA ACAGACCAAG CTTATTAGAA ATTCATGAAC AACTGGCAAA GAACATAGCC 
GTGAGCACCG GCTCAGTGGA GAGGGTGGCT AACGGAGAAA GCACTGCTGG AGAAGAGACA 
GCAGCCAGCA AGGAAGAAGA AAAGAAAACA GGATTTGTCA AGTTTGGATG GGTGAAGGGC 
GTGCTGGTAA GATGCATGCT CAATATCTGG GGTGTGATGC TTTTTATTCG ACTTTCCTGG 
ATTGTAGGTC AAGCAGGAAT TGGTCTTGGA GTAATCATAA TTCTCCTCGC CACGATGGTA 
ACCTCAATTA CTGGGTTGTC AACTTCTGCA ATAGCAACTA ATGGGTTTGT CCGTGGAGGT 
GGAGCATATT ATCTTATCTC AAGAAGTCTA GGCCCAGAGT TTGGTGGATC TATAGGACTT 
ATCTTTGCTT TTGCAAATGC TGTGGCAGTT GCTATGTATG TAGTTGGATT TGCTGAAACA 
GTCGTAGAGC TTCTTAAGGA GAGTGATACA ATAATGGTAG ATGAGTCCAA TGATATCAGA 
ATAATAGGTA CCATAACTGT TGTATGTCTT CTTGGCATCT CTGTTGCCGG CATGGAATGG 
GAAGCAAAGG CTCAAGTTAT TCTTCTAATT GTTCTCCTTA TTGCCATTGC AAACTTCTTT 
ATTGGGACAG TTATTCCTAC CAACAACGAA AAGAAGGCAA GAGGCTTTTT TAATTACCAA 
GCATCAATAT TTGCAGAAAA CTTTGGACCA GATTTCAGAA GTGGAGAAGG ATTTTTCTCA 
GTGTTTGCCA TCTTCTTCCC AGCTGCCACT GGCATTCTCG CGGGAGCCAA CATCTCAGGA 
GATCTGAAAG ATCCGCAGGG TGCGATACCC AAAGGAACAA TGCTGGCCAT TCTGATAACA 
ACAGTTGCTT ACATAGGCGT TGCAGTATGT GCAGCCTCCT GTGTTGTACG AGATGCTACT 
GGAAACGTCA ATGATACAGT TATACCTGGG ATGAGCTGCA ATGGATCATC TGCCTGCAAC 
CTAGGCTATG ATTTTTCCAG ATGCAGAAGT CAGCCATGCG ATTACGGGCT GATGAATAAT 
TTTCAGGTGA TGAGTATGGT GTCTGGCTTT GGTCCTCTCA TCACAGCTGG CATCTTTTCT 
GCTACACTGT CATCAGCTCT GGCATCTCTC GTCAGCGCAC CCAAAGTTTT TCAGGCTCTT 
TGCAAGGACA ATGTCTACAA AGGACTCCAC TTCTTTGCCA AAGGATACGG AAAAAACAAC 
GAGCCAATCA GAGGATATGT ACTCACTTTT GTGATTGCTA TGGCGTTCAT TTTGATTGCT 
CAGTTGAACA CCATTGCTCC CATCATCTCC AATTTCTTCC TGGCTTCATA TGCTTTGATT 
AATTTCTCCT GCTTTCATGC CTCATACGCA AAATCTCCAG GTTGGAGACC TGCATTCAGA 
TATTATAACA TGTGGATATC GCTTTTTGGA GCCCTGCTGT GCTGTGGCGT TATGTTTGTC 
ATCAACTGGT GGGCAGCTCT CATCACTTAT GTCATTGAAC TCTTTCTATA TATTTATGTA 
ACATATAAGA AACCAGAAGT AAATTGGGGC TCTTCTACAC AGGCTCTTTG CTATATTAAG 
GCCTTAGACA GTGCGCTGGC ATTAACTACA GTGGAAGATC ATGTGAAAAA CTTCAGACCC 
CAGTGCATAG TATTAACAGG AGCTCCTATG ATCAGGCCTG CTCTTCTAGA CATCACACAC 
AGTTTCACAA AGAACAATGG GCTGTGCATC TGCTGTGAAG TGTATACGGG ACCACGCAAG 
CTGTGTGTTA AGGAGATGAA CAGTGGCATG GCAAAAAAGC AGGCCTGGCT TACAAAGAAC 
AAAAGAAAAG CCTTTTATGC AGCAGTGGCA GCTGACAGCT TCAGAGATGG AGTCAAAAGT 
CTCCTTCAAG CCTCAGGATT GGGTAGAATG AAACCAAATA CCTTGGTGAT TGGATTTAAG 
AAGGACTGGA GAAATGCTGC TGTCACACAA GTTGAAAACT ATGTGGGTGT TATACATGAT 
GCCTTTGATT TTGAACTTGG CACGATTATT GTCAGAATCG GCCAAGGATT TGATATATCT 
CAGGTCCTCC AGGTTCAAGA GGAATTAGAG AAGCTGGAGC AGGAGAGATT GGCACTGGAA 
GCTACAATTA AAGAAAATGA ATTTGAAGAA GAAAAAAGTG GCATCTGCGG ATTCTTCAGA 
AAAGCCAGAA AGCTCAATAT CACAAAACAT GAAGCAAAAA ATGAGAGCAC CATTAACACA 
GTGCAGTCAA TGCATGTGGG AGAATTCAAT CAGAAGCTTC TAGAAGCGAG CACTCAATTT 
AAAAAGAAAC AAGGAAAAGG CACAATTGAC ATTTGGTGGC TGTTTGATGA TGGAGGTCTA 
ACAATCCTAA TTCCTTACAT TCTAACTATC AGGAAGAAGT GGAAAAATTG CAAATTAAGG 
ATCTTCACAG GAGGAAAAGT CAATAGGATT GAAGAAGAAA AACTAGTGAT GGCTTCACTT 
CTAAGCAAAT TTAGAATAAA ATTTGCTGAC ATTAATATCA TTTGTGATAT CAATATGAAA 
CCCAACAAAG AGAGTTGGAA ATTCTTTGAA GAGATGATTG AACCATATCG TCTCCGTGAA 
AGCTGCAAAG ATATAACAAC TGCTGAGAAA TTAAAGCGGG AGACTCCATG GAAAATTACA 
GATGCAGAGC TGGAAGCATT CAAAGAAAAG AGTTATCGCC AAGTTCGTTT GAATGAACTC 
TTACAAGAGC ATTCGAGGGC AGCTAATCTC ATTGTCCTGA GTCTCCCAGT GGCAAGAAAA 
GGAGTAGTGT CTGATTACCT GTATATGGCT TGGCTAGAAA TTCTCTCAAA GAACCTTCCT 
CCAATCTTAA TGGTCAGGGG AAACCACAAG AACGTACTGA CTTTCTATTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SLC12A1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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