GLI2 cDNA ORF clone, Gallus gallus(chicken)

The following GLI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa38541 XM_015289787.2
Latest version!
Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa00089 NM_001271902.1
Latest version!
Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OGa00144 NM_001271901.1
Latest version!
Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00144 XM_025152142.1
Latest version!
Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa00144 XM_015289786.2
Latest version!
Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OGa38541 XM_015289787.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa00144 XM_015289786.1 Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa38541
    Clone ID Related Accession (Same CDS sequence) XM_015289787.2 , XM_015289787.1
    Accession Version XM_015289787.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA
    GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA
    GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT
    ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG
    CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC
    CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA
    CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA
    GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA
    CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG
    AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT
    GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG
    AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT
    TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT
    GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT
    GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC
    AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG
    AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA
    GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT
    CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT
    AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG
    CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA
    GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT
    CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG
    TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG
    CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT
    CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC
    ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT
    GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG
    AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC
    AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC
    AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG
    CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT
    GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC
    AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG
    GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC
    GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC
    TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT
    GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG
    CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG
    AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG
    AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT
    GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC
    AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC
    CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC
    AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT
    CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC
    TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT
    CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA
    GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG
    AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA
    TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA
    ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG
    CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG
    TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG
    GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC
    CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA
    CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA
    GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145273.1
    CDS315..4787
    Translation

    Target ORF information:

    RefSeq Version XM_015289787.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289787.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA
    GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA
    GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT
    ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG
    CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC
    CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA
    CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA
    GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA
    CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG
    AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT
    GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG
    AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT
    TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT
    GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT
    GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC
    AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG
    AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA
    GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT
    CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT
    AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG
    CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA
    GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT
    CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG
    TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG
    CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT
    CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC
    ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT
    GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG
    AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC
    AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC
    AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG
    CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT
    GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC
    AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG
    GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC
    GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC
    TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT
    GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG
    CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG
    AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG
    AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT
    GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC
    AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC
    CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC
    AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT
    CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC
    TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT
    CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA
    GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG
    AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA
    TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA
    ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG
    CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG
    TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG
    GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC
    CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA
    CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA
    GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa38541
    Clone ID Related Accession (Same CDS sequence) XM_015289787.2 , XM_015289787.1
    Accession Version XM_015289787.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA
    GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA
    GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT
    ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG
    CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC
    CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA
    CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA
    GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA
    CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG
    AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT
    GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG
    AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT
    TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT
    GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT
    GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC
    AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG
    AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA
    GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT
    CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT
    AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG
    CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA
    GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT
    CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG
    TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG
    CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT
    CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC
    ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT
    GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG
    AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC
    AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC
    AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG
    CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT
    GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC
    AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG
    GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC
    GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC
    TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT
    GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG
    CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG
    AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG
    AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT
    GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC
    AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC
    CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC
    AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT
    CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC
    TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT
    CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA
    GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG
    AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA
    TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA
    ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG
    CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG
    TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG
    GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC
    CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA
    CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA
    GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145273.1
    CDS316..4788
    Translation

    Target ORF information:

    RefSeq Version XM_015289787.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG GAGCATCTGA CAGACCTGAA AGAAGATCTA
    GATAAGGATG AATGTAAACA GGAGCCTGAG GTTATTTATG AGACTAACTG TCACTGGGAA
    GGGTGCACAA AGGAATATGA CACTCAAGAG CAGCTCGTCC ATCACATCAA CAATGATCAT
    ATCCATGGGG AGAAGAAGGA GTTTGTCTGC CGCTGGCAGG ACTGCACGCG GGAGCAGAAG
    CCCTTCAAAG CTCAATACAT GCTGGTGGTG CACATGCGAA GGCACACTGG AGAGAAGCCC
    CACAAGTGCA CGTTTGAGGG TTGCTCCAAG GCTTACTCCC GCCTGGAGAA CTTAAAGACA
    CACCTGAGGT CCCACACTGG AGAAAAACCT TACGTCTGTG AACACGAAGG CTGCAATAAA
    GCCTTTTCCA ATGCCTCGGA TAGAGCCAAG CACCAGAACA GGACGCATTC CAATGAGAAA
    CCCTATGTCT GTAAGATCCC TGGCTGCACA AAGAGGTACA CAGACCCTAG TTCCCTCAGG
    AAACACGTCA AGACCGTACA CGGACCTGAT GCCCACGTCA CAAAGAAACA GCGCAACGAT
    GTCCACCCAA GGCCGCCTCC GCTCAAGGAA AATGGGGACA ACGAGGCGAG CGCCAAGCAG
    AGCAGTAAGG TGTCAGAGGA GAGCCCCGAG GCCAACAGTA CCACGAGGAG CATGGAGGAT
    TGCTTACAAG TCAAAACGAT AAAAACAGAG AATTCTGTGA TGTGTCAGTC CAGTCCTGGT
    GGCCAGTCAT CATGCAGCAG TGAGCCGTCA CCCCTGGGCA GCACCAACAA CAATGACAGT
    GGAGTAGAAA TGAACATGCA CAGCGGGGGA AGTCTGGGAG ATCTGACGGC GTTGGATGAC
    AACGCTCCTG TTGTGGACTC AACGGTCTCA TCTGGTAACT TGACAGTCAG CCTGCAGCTG
    AGGAAACACA TGACGACAAT GCAACGACTT GAACAGCTCA AGAAAGAGAA ACTCAAGACA
    GTTAAGGATT CCTGCTCATG GGTGAACCCA GCTCCACAAG GCAGAAACAC CAAGCTGCCT
    CCCATTTCAG GAAATGGCTC TATTCTAGAA AACAGTGGTG GTTCTTCTGC TACGCTGCCT
    AACCCAAGAA TAATGGAGCT GTCTGTCAAT GAGGTTACGA TGCTGAACCA AATCAATGAG
    CGCCGTGATA GTACGACAAG CACCATCAGC TCTGCCTACA CCGTGAGCCG CAGATCATCA
    GGGATTTCCC CATACTTCTC CAGCCGCCGC TCCAGCGAGG CTTCACAGCT CGGGCACCGT
    CCCAATAACA CGAGCTCTGC TGACTCTTAT GATCCGATTT CAACGGATGC TTCCCGCCGG
    TCAAGTGAGG CGAGCCAGTG CAGCGGGATG CCGGGCCTAC TGAACCTCAC ACCAGCTCAG
    CACTACAGGC TGAAAGCCAA GTATGCTGCT GCCACAGGGG GCCCTCCTCC AACGCCCCTT
    CCAAACATGG AGAGGATGAC CCTGAAGAAC AGAATCTCAC TTATGGATGG ACCAGAGCCC
    ACCTTGCCCT CCATTCGCCT CCCACCAGGC CCCAGGCGTT GCAGTGATGG CAACACCTAT
    GGCTACACAT CCACCGCAGC ATTTCCCCAT GAGGTTCCGG GCAACTGCAC GAGACGGGCG
    AGCGACCCTG TGAGGAGACC TGCTGGAGAG CCCCAGGCCC TCCCGAGAGT TCACCGCTTC
    AACAGCACCA ACAGCATGAA CCCCTTCCAT CCTCCACATC CTGCAGACCG GAGGAATTTC
    AGCATGCAGA GCTATGGGCG CTCTGACGGG AGTCTTGCCC GGCACACCTA CTCACCCCGG
    CCACTGAGCA TCAGTGAAAA CGTCACTATG GAAGCCATGT CTGGGGAGGC AGAGGTGCCT
    GTTGGAGATG ATGACATTAT GCTGCCAGAT GATGTGGTGC AGTACATCAA ATCTCAGAAC
    AACGGGACAG CAGCTGAGAG CACTTCCATC GGGTACAGCA ATGAGATGCA GAGCTTCCAG
    GGAGGCGGGA AGCTGCAGCC CCCAGCCTTT CCCAGCCAGC GCAGGATGGC GGTGGCCGAC
    GCGAGCATGA GCCATTTGGG GCCCATGATG GCAGAGTGTC CCATGAGCTT TGACGCGCCC
    TCAGACATGA ATAAAAATAA CATGCCTGTC CAGTGGAACG AAGTAAGCTC GGGTACAGTT
    GATCTCATGT CCAATCAATC CAAGCAGCAG TTTTCGCAAG GGAACTTAGC AGTGGTCCAG
    CAGAAGCAGA ACTTTGGTCA GTACCAGAGC TACAACCAGC AGCAGATGCA GCTACCAGAG
    AACAGCATGA ATGTAACACA GCAGAGCTTT ATGCAAAGAA ATGTGGGCAT GGATGGGCAG
    AGGCTAAACT GTATGCAGCT GCGGCAGCAG CCAATGAGCC TAGGCCCAGG GATGAACCCT
    GATCTGGGCT CGCAGGCAGG GTATAACCAA GCTCATCAGA TGCTGAGCCC CAGTGCAATC
    AATGGCAACC CAAATCAGCT CTCTCCTTCT TGTAGCAACA TGGTGACAAA GTCTGGGCCC
    CACCTCCACC CTCAGCAAAT GGACATGGCG ACCGACCCAT CCTTAATGGT CAGCAGCAAC
    AGCGAGCGCA CAATGCTGAA CCAGGTCATG CACGAACCGA GCCAGCAGAA CTACTCATCT
    CAGCCAACCC ACATGAACTT CCCCATGGCT CAGGAGGCTT TCCATCAGCC CATCATGCCC
    TCCAACCAGC AGAGCTTTGA GCCCCAGCAG AGCATGATGG GTTCGGCGGC GCAGACGTAT
    CCACCCGGCA TGGTCCAACC TCGTCCCCCC GCTGAGCCCA GCCCGGCCAA CAGGCCTCGA
    GGCCTCCGCA CCGTGCAGCA GCTGGGCTAC ATGAGAACTC CCCACCCTAC AAACACCATG
    AGCCCGGGCC AGGAGGCGGT GGAGGCTGTG CATAAAAGAA CCGGCGACGT GTTGCCAGCA
    TCGACCCAAC AGTGCGCGGA TGGCACGAGG GAAAATAATT TGATGTACTA TTATGGTCAA
    ATCCACATGT ACGAACAAAA CAGCGGCTTT GATAATCACT CGGACTGCAG GGTCAGGCAG
    CAGCAGTGCG CACTGAGCGG CAAGCCGGCC GCTCTGCCAT CCCCGGGCAC CAACCAGGTG
    TCCAGCACGG TGGACTCACA AGGTCTTGAG CCGCCCCAGA TAGATTTTGA TGCCATCATG
    GACGATGGGG ACCACTCGAG CCTGATGTCA GGAACCCTGA GCCCCAGCAT CCTGCAGAAC
    CTCTCCCAGA ACTCCTCTCG CCTGACGACT CCACGAAACT CTCTGACTCT GCCCACCATA
    CCCACGGGAA TAAGCAATAT GGCAATAGGA GATATGAGCT CCATGCTGAC CACCCTGGCA
    GAAGAGAGTA AATTTCTCAA CATGATGTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00089
    Clone ID Related Accession (Same CDS sequence) NM_001271902.1
    Accession Version NM_001271902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF022818.1, AADN05000350.1 and AM262746.1. Transcript Variant: This variant (2) differs in the 5' UTR, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcripts and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: AF022818.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201374, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGTGCC GCAGCACATT 
    TTTCCGGCCT TCCACGCTCC CTTGCCCATT GACATGCGCC ACCAGGAAGG ACGATACCAT
    TACGAGCCTC ACTCTATCCA CGCTATCCAC GGGCCTCCTC CATTGAGCGG CAGCCCCGTC
    ATCTCTGACA TCTCCCTCAT CCGTCTGTCT CCTCATCCTG CCGGGCCTGG CGAATCACCC
    TTCAGCCCAC CGCACCCCTA CGTGACGCCC CACATGGAGC ACTACCTCCG CTCTGTCCAC
    GGCAGCCCCA CGCTCTCCGT CATCTCCGCT GCCAGAGGGC TCAGCCCCGC CGATGTGGCT
    CATGAGCATC TGAAGGAGCG AGGCCTCTTC GGGCTGCCCC CACCGCCACC GGGAGCCAAC
    CCAACCGACT ACTACCACCA AATGACGCTG ATGGCCGGCC ACCCCAACCC CTACGGGGAC
    ATCCTGATGC AGAGCGGCGG AGCAGCCGGC ACGGCCCACC TGCACGAGTA CCTCAGCCCT
    GTTGATGTGT CCCGGTTTTC AAGCCCACGG GTGACTCCGA GGTTAAGCCG AAAACGAGCC
    CTGTCTATCT CCCCGCTGTC TGATGCCAGC ATTGATCTGC AGACGATGAT CAGGACCTCC
    CCAAACTCCC TCGTGGCATA TATTAACAAC TCCAGAAGCA GCTCAGCGGC AAGTGGCTCC
    TACGGCCATT TATCTGCCGG GACAATAAGC CCTGCATTCA GCTTCCCCCA CCCCATCAAC
    CCCGTGACGT ACCAGCAGAT CCTGACCCAG CAGAGAGGTC TGAGCTCAGC TTTCGGGCAC
    ACTCCTCCCC TCATCCAGCC GTCTCCCACC TTTCCCCCTC GGCAGCACAT GGCCGTCATC
    TCCGTCAACC CACCTCCGGC ACAGATCAGC AGCAACAGCA ACTGCATCTC CGACTCCAGT
    CAGAGCAAGC AGAGCAGCGA GTCAGCCGTG AGCAGCACCG TCAATCCCGT AATTAACAAG
    CGCACCAAAG TCAAGACCGA AGTCGAGGGC TTGCCCCCAG TATCCCCAAC CACGCAGGAG
    CATCTGACAG ACCTGAAAGA AGATCTAGAT AAGGATGAAT GTAAACAGGA GCCTGAGGTT
    ATTTATGAGA CTAACTGTCA CTGGGAAGGG TGCACAAAGG AATATGACAC TCAAGAGCAG
    CTCGTCCATC ACATCAACAA TGATCATATC CATGGGGAGA AGAAGGAGTT TGTCTGCCGC
    TGGCAGGACT GCACGCGGGA GCAGAAGCCC TTCAAAGCTC AATACATGCT GGTGGTGCAC
    ATGCGAAGGC ACACTGGAGA GAAGCCCCAC AAGTGCACGT TTGAGGGTTG CTCCAAGGCT
    TACTCCCGCC TGGAGAACTT AAAGACACAC CTGAGGTCCC ACACTGGAGA AAAACCTTAC
    GTCTGTGAAC ACGAAGGCTG CAATAAAGCC TTTTCCAATG CCTCGGATAG AGCCAAGCAC
    CAGAACAGGA CGCATTCCAA TGAGAAACCC TATGTCTGTA AGATCCCTGG CTGCACAAAG
    AGGTACACAG ACCCTAGTTC CCTCAGGAAA CACGTCAAGA CCGTACACGG ACCTGATGCC
    CACGTCACAA AGAAACAGCG CAACGATGTC CACCCAAGGC CGCCTCCGCT CAAGGAAAAT
    GGGGACAACG AGGCGAGCGC CAAGCAGAGC AGTAAGGTGT CAGAGGAGAG CCCCGAGGCC
    AACAGTACCA CGAGGAGCAT GGAGGATTGC TTACAAGTCA AAACGATAAA AACAGAGAAT
    TCTGTGATGT GTCAGTCCAG TCCTGGTGGC CAGTCATCAT GCAGCAGTGA GCCGTCACCC
    CTGGGCAGCA CCAACAACAA TGACAGTGGA GTAGAAATGA ACATGCACAG CGGGGGAAGT
    CTGGGAGATC TGACGGCGTT GGATGACAAC GCTCCTGTTG TGGACTCAAC GGTCTCATCT
    GGTAACTTGA CAGTCAGCCT GCAGCTGAGG AAACACATGA CGACAATGCA ACGACTTGAA
    CAGCTCAAGA AAGAGAAACT CAAGACAGTT AAGGATTCCT GCTCATGGGT GAACCCAGCT
    CCACAAGGCA GAAACACCAA GCTGCCTCCC ATTTCAGGAA ATGGCTCTAT TCTAGAAAAC
    AGTGGTGGTT CTTCTGCTAC GCTGCCTAAC CCAAGAATAA TGGAGCTGTC TGTCAATGAG
    GTTACGATGC TGAACCAAAT CAATGAGCGC CGTGATAGTA CGACAAGCAC CATCAGCTCT
    GCCTACACCG TGAGCCGCAG ATCATCAGGG ATTTCCCCAT ACTTCTCCAG CCGCCGCTCC
    AGCGAGGCTT CACAGCTCGG GCACCGTCCC AATAACACGA GCTCTGCTGA CTCTTATGAT
    CCGATTTCAA CGGATGCTTC CCGCCGGTCA AGTGAGGCGA GCCAGTGCAG CGGGATGCCG
    GGCCTACTGA ACCTCACACC AGCTCAGCAC TACAGGCTGA AAGCCAAGTA TGCTGCTGCC
    ACAGGGGGCC CTCCTCCAAC GCCCCTTCCA AACATGGAGA GGATGACCCT GAAGAACAGA
    ATCTCACTTA TGGATGGACC AGAGCCCACC TTGCCCTCCA TTCGCCTCCC ACCAGGCCCC
    AGGCGTTGCA GTGATGGCAA CACCTATGGC TACACATCCA CCGCAGCATT TCCCCATGAG
    GTTCCGGGCA ACTGCACGAG ACGGGCGAGC GACCCTGTGA GGAGACCTGC TGGAGAGCCC
    CAGGCCCTCC CGAGAGTTCA CCGCTTCAAC AGCACCAACA GCATGAACCC CTTCCATCCT
    CCACATCCTG CAGACCGGAG GAATTTCAGC ATGCAGAGCT ATGGGCGCTC TGACGGGAGT
    CTTGCCCGGC ACACCTACTC ACCCCGGCCA CTGAGCATCA GTGAAAACGT CACTATGGAA
    GCCATGTCTG GGGAGGCAGA GGTGCCTGTT GGAGATGATG ACATTATGCT GCCAGATGAT
    GTGGTGCAGT ACATCAAATC TCAGAACAAC GGGACAGCAG CTGAGAGCAC TTCCATCGGG
    TACAGCAATG AGATGCAGAG CTTCCAGGGA GGCGGGAAGC TGCAGCCCCC AGCCTTTCCC
    AGCCAGCGCA GGATGGCGGT GGCCGACGCG AGCATGAGCC ATTTGGGGCC CATGATGGCA
    GAGTGTCCCA TGAGCTTTGA CGCGCCCTCA GACATGAATA AAAATAACAT GCCTGTCCAG
    TGGAACGAAG TAAGCTCGGG TACAGTTGAT CTCATGTCCA ATCAATCCAA GCAGCAGTTT
    TCGCAAGGGA ACTTAGCAGT GGTCCAGCAG AAGCAGAACT TTGGTCAGTA CCAGAGCTAC
    AACCAGCAGC AGATGCAGCT ACCAGAGAAC AGCATGAATG TAACACAGCA GAGCTTTATG
    CAAAGAAATG TGGGCATGGA TGGGCAGAGG CTAAACTGTA TGCAGCTGCG GCAGCAGCCA
    ATGAGCCTAG GCCCAGGGAT GAACCCTGAT CTGGGCTCGC AGGCAGGGTA TAACCAAGCT
    CATCAGATGC TGAGCCCCAG TGCAATCAAT GGCAACCCAA ATCAGCTCTC TCCTTCTTGT
    AGCAACATGG TGACAAAGTC TGGGCCCCAC CTCCACCCTC AGCAAATGGA CATGGCGACC
    GACCCATCCT TAATGGTCAG CAGCAACAGC GAGCGCACAA TGCTGAACCA GGTCATGCAC
    GAACCGAGCC AGCAGAACTA CTCATCTCAG CCAACCCACA TGAACTTCCC CATGGCTCAG
    GAGGCTTTCC ATCAGCCCAT CATGCCCTCC AACCAGCAGA GCTTTGAGCC CCAGCAGAGC
    ATGATGGGTT CGGCGGCGCA GACGTATCCA CCCGGCATGG TCCAACCTCG TCCCCCCGCT
    GAGCCCAGCC CGGCCAACAG GCCTCGAGGC CTCCGCACCG TGCAGCAGCT GGGCTACATG
    AGAACTCCCC ACCCTACAAA CACCATGAGC CCGGGCCAGG AGGCGGTGGA GGCTGTGCAT
    AAAAGAACCG GCGACGTGTT GCCAGCATCG ACCCAACAGT GCGCGGATGG CACGAGGGAA
    AATAATTTGA TGTACTATTA TGGTCAAATC CACATGTACG AACAAAACAG CGGCTTTGAT
    AATCACTCGG ACTGCAGGGT CAGGCAGCAG CAGTGCGCAC TGAGCGGCAA GCCGGCCGCT
    CTGCCATCCC CGGGCACCAA CCAGGTGTCC AGCACGGTGG ACTCACAAGG TCTTGAGCCG
    CCCCAGATAG ATTTTGATGC CATCATGGAC GATGGGGACC ACTCGAGCCT GATGTCAGGA
    ACCCTGAGCC CCAGCATCCT GCAGAACCTC TCCCAGAACT CCTCTCGCCT GACGACTCCA
    CGAAACTCTC TGACTCTGCC CACCATACCC ACGGGAATAA GCAATATGGC AATAGGAGAT
    ATGAGCTCCA TGCTGACCAC CCTGGCAGAA GAGAGTAAAT TTCTCAACAT GATGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258831.1
    CDS83..4582
    Translation

    Target ORF information:

    RefSeq Version NM_001271902.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGTGCC GCAGCACATT 
    TTTCCGGCCT TCCACGCTCC CTTGCCCATT GACATGCGCC ACCAGGAAGG ACGATACCAT
    TACGAGCCTC ACTCTATCCA CGCTATCCAC GGGCCTCCTC CATTGAGCGG CAGCCCCGTC
    ATCTCTGACA TCTCCCTCAT CCGTCTGTCT CCTCATCCTG CCGGGCCTGG CGAATCACCC
    TTCAGCCCAC CGCACCCCTA CGTGACGCCC CACATGGAGC ACTACCTCCG CTCTGTCCAC
    GGCAGCCCCA CGCTCTCCGT CATCTCCGCT GCCAGAGGGC TCAGCCCCGC CGATGTGGCT
    CATGAGCATC TGAAGGAGCG AGGCCTCTTC GGGCTGCCCC CACCGCCACC GGGAGCCAAC
    CCAACCGACT ACTACCACCA AATGACGCTG ATGGCCGGCC ACCCCAACCC CTACGGGGAC
    ATCCTGATGC AGAGCGGCGG AGCAGCCGGC ACGGCCCACC TGCACGAGTA CCTCAGCCCT
    GTTGATGTGT CCCGGTTTTC AAGCCCACGG GTGACTCCGA GGTTAAGCCG AAAACGAGCC
    CTGTCTATCT CCCCGCTGTC TGATGCCAGC ATTGATCTGC AGACGATGAT CAGGACCTCC
    CCAAACTCCC TCGTGGCATA TATTAACAAC TCCAGAAGCA GCTCAGCGGC AAGTGGCTCC
    TACGGCCATT TATCTGCCGG GACAATAAGC CCTGCATTCA GCTTCCCCCA CCCCATCAAC
    CCCGTGACGT ACCAGCAGAT CCTGACCCAG CAGAGAGGTC TGAGCTCAGC TTTCGGGCAC
    ACTCCTCCCC TCATCCAGCC GTCTCCCACC TTTCCCCCTC GGCAGCACAT GGCCGTCATC
    TCCGTCAACC CACCTCCGGC ACAGATCAGC AGCAACAGCA ACTGCATCTC CGACTCCAGT
    CAGAGCAAGC AGAGCAGCGA GTCAGCCGTG AGCAGCACCG TCAATCCCGT AATTAACAAG
    CGCACCAAAG TCAAGACCGA AGTCGAGGGC TTGCCCCCAG TATCCCCAAC CACGCAGGAG
    CATCTGACAG ACCTGAAAGA AGATCTAGAT AAGGATGAAT GTAAACAGGA GCCTGAGGTT
    ATTTATGAGA CTAACTGTCA CTGGGAAGGG TGCACAAAGG AATATGACAC TCAAGAGCAG
    CTCGTCCATC ACATCAACAA TGATCATATC CATGGGGAGA AGAAGGAGTT TGTCTGCCGC
    TGGCAGGACT GCACGCGGGA GCAGAAGCCC TTCAAAGCTC AATACATGCT GGTGGTGCAC
    ATGCGAAGGC ACACTGGAGA GAAGCCCCAC AAGTGCACGT TTGAGGGTTG CTCCAAGGCT
    TACTCCCGCC TGGAGAACTT AAAGACACAC CTGAGGTCCC ACACTGGAGA AAAACCTTAC
    GTCTGTGAAC ACGAAGGCTG CAATAAAGCC TTTTCCAATG CCTCGGATAG AGCCAAGCAC
    CAGAACAGGA CGCATTCCAA TGAGAAACCC TATGTCTGTA AGATCCCTGG CTGCACAAAG
    AGGTACACAG ACCCTAGTTC CCTCAGGAAA CACGTCAAGA CCGTACACGG ACCTGATGCC
    CACGTCACAA AGAAACAGCG CAACGATGTC CACCCAAGGC CGCCTCCGCT CAAGGAAAAT
    GGGGACAACG AGGCGAGCGC CAAGCAGAGC AGTAAGGTGT CAGAGGAGAG CCCCGAGGCC
    AACAGTACCA CGAGGAGCAT GGAGGATTGC TTACAAGTCA AAACGATAAA AACAGAGAAT
    TCTGTGATGT GTCAGTCCAG TCCTGGTGGC CAGTCATCAT GCAGCAGTGA GCCGTCACCC
    CTGGGCAGCA CCAACAACAA TGACAGTGGA GTAGAAATGA ACATGCACAG CGGGGGAAGT
    CTGGGAGATC TGACGGCGTT GGATGACAAC GCTCCTGTTG TGGACTCAAC GGTCTCATCT
    GGTAACTTGA CAGTCAGCCT GCAGCTGAGG AAACACATGA CGACAATGCA ACGACTTGAA
    CAGCTCAAGA AAGAGAAACT CAAGACAGTT AAGGATTCCT GCTCATGGGT GAACCCAGCT
    CCACAAGGCA GAAACACCAA GCTGCCTCCC ATTTCAGGAA ATGGCTCTAT TCTAGAAAAC
    AGTGGTGGTT CTTCTGCTAC GCTGCCTAAC CCAAGAATAA TGGAGCTGTC TGTCAATGAG
    GTTACGATGC TGAACCAAAT CAATGAGCGC CGTGATAGTA CGACAAGCAC CATCAGCTCT
    GCCTACACCG TGAGCCGCAG ATCATCAGGG ATTTCCCCAT ACTTCTCCAG CCGCCGCTCC
    AGCGAGGCTT CACAGCTCGG GCACCGTCCC AATAACACGA GCTCTGCTGA CTCTTATGAT
    CCGATTTCAA CGGATGCTTC CCGCCGGTCA AGTGAGGCGA GCCAGTGCAG CGGGATGCCG
    GGCCTACTGA ACCTCACACC AGCTCAGCAC TACAGGCTGA AAGCCAAGTA TGCTGCTGCC
    ACAGGGGGCC CTCCTCCAAC GCCCCTTCCA AACATGGAGA GGATGACCCT GAAGAACAGA
    ATCTCACTTA TGGATGGACC AGAGCCCACC TTGCCCTCCA TTCGCCTCCC ACCAGGCCCC
    AGGCGTTGCA GTGATGGCAA CACCTATGGC TACACATCCA CCGCAGCATT TCCCCATGAG
    GTTCCGGGCA ACTGCACGAG ACGGGCGAGC GACCCTGTGA GGAGACCTGC TGGAGAGCCC
    CAGGCCCTCC CGAGAGTTCA CCGCTTCAAC AGCACCAACA GCATGAACCC CTTCCATCCT
    CCACATCCTG CAGACCGGAG GAATTTCAGC ATGCAGAGCT ATGGGCGCTC TGACGGGAGT
    CTTGCCCGGC ACACCTACTC ACCCCGGCCA CTGAGCATCA GTGAAAACGT CACTATGGAA
    GCCATGTCTG GGGAGGCAGA GGTGCCTGTT GGAGATGATG ACATTATGCT GCCAGATGAT
    GTGGTGCAGT ACATCAAATC TCAGAACAAC GGGACAGCAG CTGAGAGCAC TTCCATCGGG
    TACAGCAATG AGATGCAGAG CTTCCAGGGA GGCGGGAAGC TGCAGCCCCC AGCCTTTCCC
    AGCCAGCGCA GGATGGCGGT GGCCGACGCG AGCATGAGCC ATTTGGGGCC CATGATGGCA
    GAGTGTCCCA TGAGCTTTGA CGCGCCCTCA GACATGAATA AAAATAACAT GCCTGTCCAG
    TGGAACGAAG TAAGCTCGGG TACAGTTGAT CTCATGTCCA ATCAATCCAA GCAGCAGTTT
    TCGCAAGGGA ACTTAGCAGT GGTCCAGCAG AAGCAGAACT TTGGTCAGTA CCAGAGCTAC
    AACCAGCAGC AGATGCAGCT ACCAGAGAAC AGCATGAATG TAACACAGCA GAGCTTTATG
    CAAAGAAATG TGGGCATGGA TGGGCAGAGG CTAAACTGTA TGCAGCTGCG GCAGCAGCCA
    ATGAGCCTAG GCCCAGGGAT GAACCCTGAT CTGGGCTCGC AGGCAGGGTA TAACCAAGCT
    CATCAGATGC TGAGCCCCAG TGCAATCAAT GGCAACCCAA ATCAGCTCTC TCCTTCTTGT
    AGCAACATGG TGACAAAGTC TGGGCCCCAC CTCCACCCTC AGCAAATGGA CATGGCGACC
    GACCCATCCT TAATGGTCAG CAGCAACAGC GAGCGCACAA TGCTGAACCA GGTCATGCAC
    GAACCGAGCC AGCAGAACTA CTCATCTCAG CCAACCCACA TGAACTTCCC CATGGCTCAG
    GAGGCTTTCC ATCAGCCCAT CATGCCCTCC AACCAGCAGA GCTTTGAGCC CCAGCAGAGC
    ATGATGGGTT CGGCGGCGCA GACGTATCCA CCCGGCATGG TCCAACCTCG TCCCCCCGCT
    GAGCCCAGCC CGGCCAACAG GCCTCGAGGC CTCCGCACCG TGCAGCAGCT GGGCTACATG
    AGAACTCCCC ACCCTACAAA CACCATGAGC CCGGGCCAGG AGGCGGTGGA GGCTGTGCAT
    AAAAGAACCG GCGACGTGTT GCCAGCATCG ACCCAACAGT GCGCGGATGG CACGAGGGAA
    AATAATTTGA TGTACTATTA TGGTCAAATC CACATGTACG AACAAAACAG CGGCTTTGAT
    AATCACTCGG ACTGCAGGGT CAGGCAGCAG CAGTGCGCAC TGAGCGGCAA GCCGGCCGCT
    CTGCCATCCC CGGGCACCAA CCAGGTGTCC AGCACGGTGG ACTCACAAGG TCTTGAGCCG
    CCCCAGATAG ATTTTGATGC CATCATGGAC GATGGGGACC ACTCGAGCCT GATGTCAGGA
    ACCCTGAGCC CCAGCATCCT GCAGAACCTC TCCCAGAACT CCTCTCGCCT GACGACTCCA
    CGAAACTCTC TGACTCTGCC CACCATACCC ACGGGAATAA GCAATATGGC AATAGGAGAT
    ATGAGCTCCA TGCTGACCAC CCTGGCAGAA GAGAGTAAAT TTCTCAACAT GATGTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00144
    Clone ID Related Accession (Same CDS sequence) NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
    Accession Version NM_001271901.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AM262746.1, AADN05000350.1 and AF022818.1. On Dec 13, 2012 this sequence version replaced XM_003641584.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcripts of different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2201374, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001258830.1
    CDS99..4685
    Translation

    Target ORF information:

    RefSeq Version NM_001271901.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001271901.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00144
    Clone ID Related Accession (Same CDS sequence) NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
    Accession Version XM_025152142.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025007910.1
    CDS246..4832
    Translation

    Target ORF information:

    RefSeq Version XM_025152142.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025152142.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00144
    Clone ID Related Accession (Same CDS sequence) NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
    Accession Version XM_015289786.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145272.1
    CDS315..4901
    Translation

    Target ORF information:

    RefSeq Version XM_015289786.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00144
    Clone ID Related Accession (Same CDS sequence) NM_001271901.1 , XM_025152142.1 , XM_015289786.1 , XM_015289786.2
    Accession Version XM_015289786.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product zinc finger protein GLI2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289786.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145272.1
    CDS314..4900
    Translation

    Target ORF information:

    RefSeq Version XM_015289786.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus GLI family zinc finger 2 (GLI2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289786.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAAACTT CTGCTTCAAC TGTTGCTGGG AAAAAAGAAG GCAAAGGTGT GGTTCTGGAG 
    GGGGATGGCT TTACGGAGAC GGGGAAGAAA CCCACTCCTT TAGCAGCTGC AGGAGCAGCA
    GTGGCACAAG GAGTGCCGCA GCACATTTTT CCGGCCTTCC ACGCTCCCTT GCCCATTGAC
    ATGCGCCACC AGGAAGGACG ATACCATTAC GAGCCTCACT CTATCCACGC TATCCACGGG
    CCTCCTCCAT TGAGCGGCAG CCCCGTCATC TCTGACATCT CCCTCATCCG TCTGTCTCCT
    CATCCTGCCG GGCCTGGCGA ATCACCCTTC AGCCCACCGC ACCCCTACGT GACGCCCCAC
    ATGGAGCACT ACCTCCGCTC TGTCCACGGC AGCCCCACGC TCTCCGTCAT CTCCGCTGCC
    AGAGGGCTCA GCCCCGCCGA TGTGGCTCAT GAGCATCTGA AGGAGCGAGG CCTCTTCGGG
    CTGCCCCCAC CGCCACCGGG AGCCAACCCA ACCGACTACT ACCACCAAAT GACGCTGATG
    GCCGGCCACC CCAACCCCTA CGGGGACATC CTGATGCAGA GCGGCGGAGC AGCCGGCACG
    GCCCACCTGC ACGAGTACCT CAGCCCTGTT GATGTGTCCC GGTTTTCAAG CCCACGGGTG
    ACTCCGAGGT TAAGCCGAAA ACGAGCCCTG TCTATCTCCC CGCTGTCTGA TGCCAGCATT
    GATCTGCAGA CGATGATCAG GACCTCCCCA AACTCCCTCG TGGCATATAT TAACAACTCC
    AGAAGCAGCT CAGCGGCAAG TGGCTCCTAC GGCCATTTAT CTGCCGGGAC AATAAGCCCT
    GCATTCAGCT TCCCCCACCC CATCAACCCC GTGACGTACC AGCAGATCCT GACCCAGCAG
    AGAGGTCTGA GCTCAGCTTT CGGGCACACT CCTCCCCTCA TCCAGCCGTC TCCCACCTTT
    CCCCCTCGGC AGCACATGGC CGTCATCTCC GTCAACCCAC CTCCGGCACA GATCAGCAGC
    AACAGCAACT GCATCTCCGA CTCCAGTCAG AGCAAGCAGA GCAGCGAGTC AGCCGTGAGC
    AGCACCGTCA ATCCCGTAAT TAACAAGCGC ACCAAAGTCA AGACCGAAGT CGAGGGCTTG
    CCCCCAGTAT CCCCAACCAC GCAGGAGCAT CTGACAGACC TGAAAGAAGA TCTAGATAAG
    GATGAATGTA AACAGGAGCC TGAGGTTATT TATGAGACTA ACTGTCACTG GGAAGGGTGC
    ACAAAGGAAT ATGACACTCA AGAGCAGCTC GTCCATCACA TCAACAATGA TCATATCCAT
    GGGGAGAAGA AGGAGTTTGT CTGCCGCTGG CAGGACTGCA CGCGGGAGCA GAAGCCCTTC
    AAAGCTCAAT ACATGCTGGT GGTGCACATG CGAAGGCACA CTGGAGAGAA GCCCCACAAG
    TGCACGTTTG AGGGTTGCTC CAAGGCTTAC TCCCGCCTGG AGAACTTAAA GACACACCTG
    AGGTCCCACA CTGGAGAAAA ACCTTACGTC TGTGAACACG AAGGCTGCAA TAAAGCCTTT
    TCCAATGCCT CGGATAGAGC CAAGCACCAG AACAGGACGC ATTCCAATGA GAAACCCTAT
    GTCTGTAAGA TCCCTGGCTG CACAAAGAGG TACACAGACC CTAGTTCCCT CAGGAAACAC
    GTCAAGACCG TACACGGACC TGATGCCCAC GTCACAAAGA AACAGCGCAA CGATGTCCAC
    CCAAGGCCGC CTCCGCTCAA GGAAAATGGG GACAACGAGG CGAGCGCCAA GCAGAGCAGT
    AAGGTGTCAG AGGAGAGCCC CGAGGCCAAC AGTACCACGA GGAGCATGGA GGATTGCTTA
    CAAGTCAAAA CGATAAAAAC AGAGAATTCT GTGATGTGTC AGTCCAGTCC TGGTGGCCAG
    TCATCATGCA GCAGTGAGCC GTCACCCCTG GGCAGCACCA ACAACAATGA CAGTGGAGTA
    GAAATGAACA TGCACAGCGG GGGAAGTCTG GGAGATCTGA CGGCGTTGGA TGACAACGCT
    CCTGTTGTGG ACTCAACGGT CTCATCTGGT AACTTGACAG TCAGCCTGCA GCTGAGGAAA
    CACATGACGA CAATGCAACG ACTTGAACAG CTCAAGAAAG AGAAACTCAA GACAGTTAAG
    GATTCCTGCT CATGGGTGAA CCCAGCTCCA CAAGGCAGAA ACACCAAGCT GCCTCCCATT
    TCAGGAAATG GCTCTATTCT AGAAAACAGT GGTGGTTCTT CTGCTACGCT GCCTAACCCA
    AGAATAATGG AGCTGTCTGT CAATGAGGTT ACGATGCTGA ACCAAATCAA TGAGCGCCGT
    GATAGTACGA CAAGCACCAT CAGCTCTGCC TACACCGTGA GCCGCAGATC ATCAGGGATT
    TCCCCATACT TCTCCAGCCG CCGCTCCAGC GAGGCTTCAC AGCTCGGGCA CCGTCCCAAT
    AACACGAGCT CTGCTGACTC TTATGATCCG ATTTCAACGG ATGCTTCCCG CCGGTCAAGT
    GAGGCGAGCC AGTGCAGCGG GATGCCGGGC CTACTGAACC TCACACCAGC TCAGCACTAC
    AGGCTGAAAG CCAAGTATGC TGCTGCCACA GGGGGCCCTC CTCCAACGCC CCTTCCAAAC
    ATGGAGAGGA TGACCCTGAA GAACAGAATC TCACTTATGG ATGGACCAGA GCCCACCTTG
    CCCTCCATTC GCCTCCCACC AGGCCCCAGG CGTTGCAGTG ATGGCAACAC CTATGGCTAC
    ACATCCACCG CAGCATTTCC CCATGAGGTT CCGGGCAACT GCACGAGACG GGCGAGCGAC
    CCTGTGAGGA GACCTGCTGG AGAGCCCCAG GCCCTCCCGA GAGTTCACCG CTTCAACAGC
    ACCAACAGCA TGAACCCCTT CCATCCTCCA CATCCTGCAG ACCGGAGGAA TTTCAGCATG
    CAGAGCTATG GGCGCTCTGA CGGGAGTCTT GCCCGGCACA CCTACTCACC CCGGCCACTG
    AGCATCAGTG AAAACGTCAC TATGGAAGCC ATGTCTGGGG AGGCAGAGGT GCCTGTTGGA
    GATGATGACA TTATGCTGCC AGATGATGTG GTGCAGTACA TCAAATCTCA GAACAACGGG
    ACAGCAGCTG AGAGCACTTC CATCGGGTAC AGCAATGAGA TGCAGAGCTT CCAGGGAGGC
    GGGAAGCTGC AGCCCCCAGC CTTTCCCAGC CAGCGCAGGA TGGCGGTGGC CGACGCGAGC
    ATGAGCCATT TGGGGCCCAT GATGGCAGAG TGTCCCATGA GCTTTGACGC GCCCTCAGAC
    ATGAATAAAA ATAACATGCC TGTCCAGTGG AACGAAGTAA GCTCGGGTAC AGTTGATCTC
    ATGTCCAATC AATCCAAGCA GCAGTTTTCG CAAGGGAACT TAGCAGTGGT CCAGCAGAAG
    CAGAACTTTG GTCAGTACCA GAGCTACAAC CAGCAGCAGA TGCAGCTACC AGAGAACAGC
    ATGAATGTAA CACAGCAGAG CTTTATGCAA AGAAATGTGG GCATGGATGG GCAGAGGCTA
    AACTGTATGC AGCTGCGGCA GCAGCCAATG AGCCTAGGCC CAGGGATGAA CCCTGATCTG
    GGCTCGCAGG CAGGGTATAA CCAAGCTCAT CAGATGCTGA GCCCCAGTGC AATCAATGGC
    AACCCAAATC AGCTCTCTCC TTCTTGTAGC AACATGGTGA CAAAGTCTGG GCCCCACCTC
    CACCCTCAGC AAATGGACAT GGCGACCGAC CCATCCTTAA TGGTCAGCAG CAACAGCGAG
    CGCACAATGC TGAACCAGGT CATGCACGAA CCGAGCCAGC AGAACTACTC ATCTCAGCCA
    ACCCACATGA ACTTCCCCAT GGCTCAGGAG GCTTTCCATC AGCCCATCAT GCCCTCCAAC
    CAGCAGAGCT TTGAGCCCCA GCAGAGCATG ATGGGTTCGG CGGCGCAGAC GTATCCACCC
    GGCATGGTCC AACCTCGTCC CCCCGCTGAG CCCAGCCCGG CCAACAGGCC TCGAGGCCTC
    CGCACCGTGC AGCAGCTGGG CTACATGAGA ACTCCCCACC CTACAAACAC CATGAGCCCG
    GGCCAGGAGG CGGTGGAGGC TGTGCATAAA AGAACCGGCG ACGTGTTGCC AGCATCGACC
    CAACAGTGCG CGGATGGCAC GAGGGAAAAT AATTTGATGT ACTATTATGG TCAAATCCAC
    ATGTACGAAC AAAACAGCGG CTTTGATAAT CACTCGGACT GCAGGGTCAG GCAGCAGCAG
    TGCGCACTGA GCGGCAAGCC GGCCGCTCTG CCATCCCCGG GCACCAACCA GGTGTCCAGC
    ACGGTGGACT CACAAGGTCT TGAGCCGCCC CAGATAGATT TTGATGCCAT CATGGACGAT
    GGGGACCACT CGAGCCTGAT GTCAGGAACC CTGAGCCCCA GCATCCTGCA GAACCTCTCC
    CAGAACTCCT CTCGCCTGAC GACTCCACGA AACTCTCTGA CTCTGCCCAC CATACCCACG
    GGAATAAGCA ATATGGCAAT AGGAGATATG AGCTCCATGC TGACCACCCT GGCAGAAGAG
    AGTAAATTTC TCAACATGAT GTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.