CACNA1D cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1D
Entrez Gene ID	395895
Full Name	calcium voltage-gated channel subunit alpha1 D
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 D Names voltage-dependent L-type calcium channel subunit alpha-1D CHCACHA1D L-type voltage-gated calcium channel alpha1D subunit ChCaChA1D calcium channel, voltage-dependent, L type, alpha 1D subunit voltage-gated calcium channel subunit alpha Cav1.3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)
Summary	This gene encodes a member of the voltage-dependent calcium channel protein subunit family. The cDNA for this gene was cloned from hair cells of the cochlea in the chicken inner ear. Study of the similar transmembrane protein in deficient mice reported impaired cardiac function and deafness, suggesting that the encoded protein is required for normal hearing and pacemaker activity in the heart. [provided by RefSeq, Aug 2012].

mRNA and Protein(s)

mRNA	Protein	Name
XM_015292976.1	XP_015148462.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_015292978.1	XP_015148464.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_015292985.1	XP_015148471.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_015292996.1	XP_015148482.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X22
XM_015292998.1	XP_015148484.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X24
XM_015293000.1	XP_015148486.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X26
XM_015292981.2	XP_015148467.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_015292999.2	XP_015148485.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
XM_015292990.2	XP_015148476.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
XM_025154431.1	XP_025010199.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X26
XM_015292996.2	XP_015148482.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X28
XM_015293004.2	XP_015148490.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X37
XM_015292987.1	XP_015148473.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_015292988.1	XP_015148474.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_015292990.1	XP_015148476.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_015292994.1	XP_015148480.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
XM_015293005.1	XP_015148491.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X31
XM_025154428.1	XP_025010196.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_015292977.2	XP_015148463.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_015292978.2	XP_015148464.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_015292980.2	XP_015148466.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_015292983.2	XP_015148469.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_015292989.2	XP_015148475.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
XM_015292993.2	XP_015148479.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X24
XM_025154432.1	XP_025010200.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X29
NM_205034.2	NP_990365.1	voltage-dependent L-type calcium channel subunit alpha-1D
XM_015292975.1	XP_015148461.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_015292977.1	XP_015148463.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_015292979.1	XP_015148465.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_015292982.1	XP_015148468.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_015292984.1	XP_015148470.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
XM_015292999.1	XP_015148485.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X25
XM_015293001.1	XP_015148487.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X27
XM_015293004.1	XP_015148490.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X30
XM_015292979.2	XP_015148465.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_025154430.1	XP_025010198.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
XM_015292984.2	XP_015148470.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
XM_015292986.2	XP_015148472.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
XM_015292991.2	XP_015148477.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X22
XM_025154433.1	XP_025010201.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X30
XM_015292986.1	XP_015148472.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_015292993.1	XP_015148479.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
XM_015293002.1	XP_015148488.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X28
XM_015293003.1	XP_015148489.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X29
XM_015292975.2	XP_015148461.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_025154429.1	XP_025010197.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_015292982.2	XP_015148468.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_015292985.2	XP_015148471.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_015292987.2	XP_015148473.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
XM_015292988.2	XP_015148474.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
XM_015292992.2	XP_015148478.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X23
XM_015292995.2	XP_015148481.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X27
XM_015292997.2	XP_015148483.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X31
XM_015293001.2	XP_015148487.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X34
XM_015293002.2	XP_015148488.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X35
XM_015293003.2	XP_015148489.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X36
XM_015292980.1	XP_015148466.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_015292981.1	XP_015148467.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_015292983.1	XP_015148469.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_015292991.1	XP_015148477.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
XM_015292989.1	XP_015148475.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
XM_015292992.1	XP_015148478.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
XM_015292995.1	XP_015148481.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
XM_015292997.1	XP_015148483.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X23
XM_015292976.2	XP_015148462.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_025154427.1	XP_025010195.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_015292994.2	XP_015148480.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X25
XM_015292998.2	XP_015148484.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X32
XM_015293000.2	XP_015148486.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X33
XM_015293005.2	XP_015148491.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X38

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Gene Ontology
Bibliography

Related articles in PubMed

Development and regeneration of hair cells share common functional features.
Levic S, Nie L, Tuteja D, Harvey M, Sokolowski BH, Yamoah EN
Proceedings of the National Academy of Sciences of the United States of America104(48)19108-13(2007 Nov)

Predominance of the alpha1D subunit in L-type voltage-gated Ca2+ channels of hair cells in the chicken's cochlea.
Kollmar R, Montgomery LG, Fak J, Henry LJ, Hudspeth AJ
Proceedings of the National Academy of Sciences of the United States of America94(26)14883-8(1997 Dec)

Hair cell-specific splicing of mRNA for the alpha1D subunit of voltage-gated Ca2+ channels in the chicken's cochlea.
Kollmar R, Fak J, Montgomery LG, Hudspeth AJ
Proceedings of the National Academy of Sciences of the United States of America94(26)14889-93(1997 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A transient Ca(2+) current produced by the Ca(v)3.1 channel is expressed early in development to initiate spontaneous Ca(2+) spikes. Ca(v)1.3 currents, typical of mature hair cells, appeared later in development.
Title: Development and regeneration of hair cells share common functional features.

The following CACNA1D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa143890	XM_025154432.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa143885	XM_025154427.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa143886	XM_025154428.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa143887	XM_025154429.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa143888	XM_025154430.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa143889	XM_025154431.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa143891	XM_025154433.1 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa41333	XM_015292975.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41334	XM_015292976.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41335	XM_015292977.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41336	XM_015292978.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41337	XM_015292979.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41338	XM_015292980.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41339	XM_015292981.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41340	XM_015292982.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41341	XM_015292983.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41342	XM_015292984.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41343	XM_015292985.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41344	XM_015292986.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41345	XM_015292987.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41346	XM_015292988.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41347	XM_015292989.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41348	XM_015292990.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41349	XM_015292991.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41350	XM_015292992.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41351	XM_015292993.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41352	XM_015292994.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41353	XM_015292995.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41354	XM_015292996.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41355	XM_015292997.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41356	XM_015292998.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41357	XM_015292999.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41358	XM_015293000.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41359	XM_015293001.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41360	XM_015293002.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41361	XM_015293003.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41362	XM_015293004.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41363	XM_015293005.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa28272	NM_205034.2 Latest version!	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OGa41354	XM_015292996.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41348	XM_015292990.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41336	XM_015292978.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41335	XM_015292977.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41357	XM_015292999.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41362	XM_015293004.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41337	XM_015292979.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41342	XM_015292984.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41344	XM_015292986.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41351	XM_015292993.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41333	XM_015292975.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41340	XM_015292982.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41343	XM_015292985.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41345	XM_015292987.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41346	XM_015292988.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41359	XM_015293001.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41360	XM_015293002.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41361	XM_015293003.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41338	XM_015292980.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41339	XM_015292981.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41341	XM_015292983.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41349	XM_015292991.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41347	XM_015292989.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41350	XM_015292992.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41353	XM_015292995.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41355	XM_015292997.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41334	XM_015292976.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41352	XM_015292994.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41356	XM_015292998.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41358	XM_015293000.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa41363	XM_015293005.1	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1D ( XM_025154432.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010200.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X29, mRNA.

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CloneID	OGa143890
Clone ID Related Accession (Same CDS sequence)	XM_025154432.1
Accession Version	XM_025154432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTATA CTGGGGAATT TTCCAAGGAA AGAGAAAAAG CCAAGGCCCG AGGGGACTTT 
CAGAAGCTAC GTGAAAAACA ACAGCTTGAA GAAGATCTGA AGGGATATTT AGACTGGATT 
ACACAGGCAG AAGACATTGA TCCTGAGAAT GATGAAGAGG CTGATGAAGA AGGGAAACGG 
AACAGGGTTA CACTGGCTGA CTTAATGGAA GAAAAGAAGA AAAGCAGACT TAGTTGCTTT 
GGCCGGTCTT CCAATAAACA TGCAAGCATG CCCACCAGCG AAACCGAGTC AGTGAACACA 
GAGAATGTGA GTGGAGAAGG AGAGAACCCA GCATGCTGTG GGAGCTTATG TCAAACCATC 
TCAAAATCCA AGTTCAGTCG ACGCTGGCGT CGCTGGAATC GATTTAATCG AAGAAAATGT 
AGAGCTGCAG TAAAATCTGT CACGTTTTAT TGGCTTGTTA TTGTCTTGGT CTTTCTGAAC 
ACATTAACTA TCTCATCGGA ACATTATAAC CAACCTGACT GGCTGACCCA GATCCAAGAT 
ATCGCAAATA AAGTTCTTCT GGCTTTGTTC ACCTGTGAAA TGTTAGTAAA GATGTACAGC 
TTGGGTCTAC AGGCTTATTT TGTTTCTCTT TTTAACCGCT TTGATTGCTT CGTTGTTTGT 
GGTGGCATTG TTGAAACCAT TTTGGTGGAG TTGGAAATTA TGTCACCCCT TGGAATTTCA 
GTGTTTCGCT GTGTTCGTCT CCTGCGTATT TTTAAAGTAA CAAGGCACTG GGCATCCCTG 
AGCAACTTGG TAGCATCCTT GTTAAACTCA ATGAAGTCCA TTGCTTCACT GTTGCTTCTG 
CTTTTCCTCT TCATCATAAT CTTCTCGTTG CTTGGAATGC AGCTGTTTGG AGGCAAATTT 
AATTTTGATG AAACTCAAAC AAAACGGAGC ACCTTCGATA ATTTTCCACA AGCTCTTTTA 
ACTGTATTCC AGATTCTAAC AGGTGAAGAC TGGAATGCTG TTATGTATGA TGGCATAATG 
GCGTATGGTG GCCCATCATC GTCAGGCATG ATAGTCTGCA TATATTTCAT TATCCTCTTC 
ATCTGCGGTA ACTATATCTT GCTAAATGTC TTCTTGGCCA TTGCTGTGGA TAACTTGGCA 
GATGCTGAAA GTCTAAATAC AGCTCAGAAA GAAGAAGCAG AAGAAAAGGA AAGGAAAAAG 
AATGCAAGAA AAGAAAGTCT GGAAAATAAA AAAAGTGAGA AGTCAGAAGG TGACCAAAAG 
AAGCCCAAGG ATAGTAAGGT GACAATTGCT GAATATGGAG AAGGAGAGGA TGAAGATAAA 
GATCCCTATC CACCCTGTGA TGTACCAGTA GGTGAAGATG AAGAAGATGA GGAGGATGAA 
CCTGAGGTAC CAGCAGGCCC ACGTCCCCGG AGAATATCAG AACTAAACAT GAAGGAGAAG 
ATAACACCGA TCCCTGAAGG GAGTGCCTTC TTCATTTTCA GCAGCACCAA TCCAATTCGA 
GTGGGATGCC ACAGGCTCAT CAATCACCAC ATCTTTACCA ATCTCATCCT TGTCTTCATC 
ATGCTGAGCA GTGTTTCCCT AGCAGCAGAA GATCCGATTC GCAGCCACTC TTTTCGAAAT 
AATATACTTG GTTACTTTGA CTATGCTTTC ACAGCCATCT TTACTGTTGA AATCCTGTTA 
AAGATCCTAG GGTATGCAGA TTATGTCTTC ACTAGTATGT TTACATTTGA GATCATTTTG 
AAGATGACAG CTTTCGGAGC ATTCCTTCAT AAAGGGTCCT TCTGCAGAAA TTACTTTAAT 
TTGCTGGATT TGCTGGTTGT GGGTGTTTCT CTGGTATCAT TTGGTATTCA GTCAAGTGCT 
ATCTCAGTTG TGAAGATCCT CAGAGTTTTG AGAGTCTTGA GGCCCCTTAG GGCTATAAAC 
AGAGCAAAAG GACTTAAGCA CGTTGTTCAG TGCGTCTTTG TGGCTATTAG AACTATTGGT 
AATATCATGA TTGTTACAAC TCTGCTGCAG TTCATGTTTG CTTGTATTGG AGTGCAGCTC 
TTCAAGGGAA AATTCTACAA GTGCACTGAT GAGGCAAAAC AGAATCCAGA GGAATGCCGG 
GGGATATACA TTGTTTATAA AGATGGAGAT GTTGATAATC CAATGGTCAA AGAGAGGGTC 
TGGCAAAATA GTGATTTCAA CTTCGACAAT GTTCTGTCTG CTATGATGGC CCTTTTCACA 
GTGTCTACTT TTGAAGGCTG GCCAGCGCTG CTGTACAAAG CCATTGATTC CAATGGCGAG 
AATGTAGGAC CTGTGTACAA CTACAGAGTG GAGATCTCCA TTTTTTTCAT CATCTACATC 
ATCATTATTG CTTTCTTTAT GATGAACATA TTTGTTGGTT TTGTCATTGT TACCTTTCAA 
GAACAGGGAG AACAAGAATA CAAGAACTGT GAGCTGGACA AAAATCAGCG TCAGTGCGTG 
GAATATGCCT TGAAGGCTCG TCCTCTTCGT AGATACATTC CAAAAAATCC TTACCAGTAT 
AAGTTCTGGT ATGTGGTGAA TTCTACTGGA TTTGAATACA TCATGTTTGT CCTCATCATG 
CTGAACACAC TTTGCCTGGC TATGCAGCAC TATGGACAGT CTAAGCTCTT TAATGATGCG 
ATGGACATTA TGAATATGGT TTTCACGGGA GTGTTCACTG TTGAAATGGT TTTGAAGCTG 
ATTGCATTCA AACCCAAGAT TTTTGTAAGA AAAAAGGAAA GATGGCTAGG CTATTTTAGT 
GATGCCTGGA ATACGTTTGA CTCCCTCATC GTTATTGGCA GCATAGTAGA CGTCGTTCTC 
AGTGAAGCTG ATCACTATTT CACTGATGCA TGGAACACTT TTGATGCCTT AATTGTTGTT 
GGTAGCGTCG TTGATATTGC TATAACAGAA GTTAATCCAA AGCCAACTGA AACAGTCACA 
ACTGATGAGT CTGGGAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010200.1
CDS	152..5677
Translation	MCYTGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154432.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154432.1

ORF Insert Sequence:

ATGTGTTATA CTGGGGAATT TTCCAAGGAA AGAGAAAAAG CCAAGGCCCG AGGGGACTTT 
CAGAAGCTAC GTGAAAAACA ACAGCTTGAA GAAGATCTGA AGGGATATTT AGACTGGATT 
ACACAGGCAG AAGACATTGA TCCTGAGAAT GATGAAGAGG CTGATGAAGA AGGGAAACGG 
AACAGGGTTA CACTGGCTGA CTTAATGGAA GAAAAGAAGA AAAGCAGACT TAGTTGCTTT 
GGCCGGTCTT CCAATAAACA TGCAAGCATG CCCACCAGCG AAACCGAGTC AGTGAACACA 
GAGAATGTGA GTGGAGAAGG AGAGAACCCA GCATGCTGTG GGAGCTTATG TCAAACCATC 
TCAAAATCCA AGTTCAGTCG ACGCTGGCGT CGCTGGAATC GATTTAATCG AAGAAAATGT 
AGAGCTGCAG TAAAATCTGT CACGTTTTAT TGGCTTGTTA TTGTCTTGGT CTTTCTGAAC 
ACATTAACTA TCTCATCGGA ACATTATAAC CAACCTGACT GGCTGACCCA GATCCAAGAT 
ATCGCAAATA AAGTTCTTCT GGCTTTGTTC ACCTGTGAAA TGTTAGTAAA GATGTACAGC 
TTGGGTCTAC AGGCTTATTT TGTTTCTCTT TTTAACCGCT TTGATTGCTT CGTTGTTTGT 
GGTGGCATTG TTGAAACCAT TTTGGTGGAG TTGGAAATTA TGTCACCCCT TGGAATTTCA 
GTGTTTCGCT GTGTTCGTCT CCTGCGTATT TTTAAAGTAA CAAGGCACTG GGCATCCCTG 
AGCAACTTGG TAGCATCCTT GTTAAACTCA ATGAAGTCCA TTGCTTCACT GTTGCTTCTG 
CTTTTCCTCT TCATCATAAT CTTCTCGTTG CTTGGAATGC AGCTGTTTGG AGGCAAATTT 
AATTTTGATG AAACTCAAAC AAAACGGAGC ACCTTCGATA ATTTTCCACA AGCTCTTTTA 
ACTGTATTCC AGATTCTAAC AGGTGAAGAC TGGAATGCTG TTATGTATGA TGGCATAATG 
GCGTATGGTG GCCCATCATC GTCAGGCATG ATAGTCTGCA TATATTTCAT TATCCTCTTC 
ATCTGCGGTA ACTATATCTT GCTAAATGTC TTCTTGGCCA TTGCTGTGGA TAACTTGGCA 
GATGCTGAAA GTCTAAATAC AGCTCAGAAA GAAGAAGCAG AAGAAAAGGA AAGGAAAAAG 
AATGCAAGAA AAGAAAGTCT GGAAAATAAA AAAAGTGAGA AGTCAGAAGG TGACCAAAAG 
AAGCCCAAGG ATAGTAAGGT GACAATTGCT GAATATGGAG AAGGAGAGGA TGAAGATAAA 
GATCCCTATC CACCCTGTGA TGTACCAGTA GGTGAAGATG AAGAAGATGA GGAGGATGAA 
CCTGAGGTAC CAGCAGGCCC ACGTCCCCGG AGAATATCAG AACTAAACAT GAAGGAGAAG 
ATAACACCGA TCCCTGAAGG GAGTGCCTTC TTCATTTTCA GCAGCACCAA TCCAATTCGA 
GTGGGATGCC ACAGGCTCAT CAATCACCAC ATCTTTACCA ATCTCATCCT TGTCTTCATC 
ATGCTGAGCA GTGTTTCCCT AGCAGCAGAA GATCCGATTC GCAGCCACTC TTTTCGAAAT 
AATATACTTG GTTACTTTGA CTATGCTTTC ACAGCCATCT TTACTGTTGA AATCCTGTTA 
AAGATCCTAG GGTATGCAGA TTATGTCTTC ACTAGTATGT TTACATTTGA GATCATTTTG 
AAGATGACAG CTTTCGGAGC ATTCCTTCAT AAAGGGTCCT TCTGCAGAAA TTACTTTAAT 
TTGCTGGATT TGCTGGTTGT GGGTGTTTCT CTGGTATCAT TTGGTATTCA GTCAAGTGCT 
ATCTCAGTTG TGAAGATCCT CAGAGTTTTG AGAGTCTTGA GGCCCCTTAG GGCTATAAAC 
AGAGCAAAAG GACTTAAGCA CGTTGTTCAG TGCGTCTTTG TGGCTATTAG AACTATTGGT 
AATATCATGA TTGTTACAAC TCTGCTGCAG TTCATGTTTG CTTGTATTGG AGTGCAGCTC 
TTCAAGGGAA AATTCTACAA GTGCACTGAT GAGGCAAAAC AGAATCCAGA GGAATGCCGG 
GGGATATACA TTGTTTATAA AGATGGAGAT GTTGATAATC CAATGGTCAA AGAGAGGGTC 
TGGCAAAATA GTGATTTCAA CTTCGACAAT GTTCTGTCTG CTATGATGGC CCTTTTCACA 
GTGTCTACTT TTGAAGGCTG GCCAGCGCTG CTGTACAAAG CCATTGATTC CAATGGCGAG 
AATGTAGGAC CTGTGTACAA CTACAGAGTG GAGATCTCCA TTTTTTTCAT CATCTACATC 
ATCATTATTG CTTTCTTTAT GATGAACATA TTTGTTGGTT TTGTCATTGT TACCTTTCAA 
GAACAGGGAG AACAAGAATA CAAGAACTGT GAGCTGGACA AAAATCAGCG TCAGTGCGTG 
GAATATGCCT TGAAGGCTCG TCCTCTTCGT AGATACATTC CAAAAAATCC TTACCAGTAT 
AAGTTCTGGT ATGTGGTGAA TTCTACTGGA TTTGAATACA TCATGTTTGT CCTCATCATG 
CTGAACACAC TTTGCCTGGC TATGCAGCAC TATGGACAGT CTAAGCTCTT TAATGATGCG 
ATGGACATTA TGAATATGGT TTTCACGGGA GTGTTCACTG TTGAAATGGT TTTGAAGCTG 
ATTGCATTCA AACCCAAGAT TTTTGTAAGA AAAAAGGAAA GATGGCTAGG CTATTTTAGT 
GATGCCTGGA ATACGTTTGA CTCCCTCATC GTTATTGGCA GCATAGTAGA CGTCGTTCTC 
AGTGAAGCTG ATCACTATTT CACTGATGCA TGGAACACTT TTGATGCCTT AATTGTTGTT 
GGTAGCGTCG TTGATATTGC TATAACAGAA GTTAATCCAA AGCCAACTGA AACAGTCACA 
ACTGATGAGT CTGGGAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_025154427.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010195.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

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CloneID	OGa143885
Clone ID Related Accession (Same CDS sequence)	XM_025154427.1
Accession Version	XM_025154427.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGG 
TCTGACTCTG GTGATGGCCG TCGACCAACT ATTTGCCGTG AAGAACGTGA CATTCGAGAC 
TATTGCAATG ATGATCATTA TTTGGGAGAG CAGGAATATT ATAGTGGAGA AGAATACTAT 
GAGGAAGACA GCATGTTGTC AGGAAACAGA CATGTGTATG ATTACCACTG TAGACATCAC 
TGCCATGACT CAGATTTTGA GAGACCAAAA GGTTACCATC ACCCACATGG GTTTTTTGAG 
GAAGATGATT CACAGACCTG TTATGATACG AAAAGATCTC CTAGGAGACG GCTGCTACCT 
CCAACACCAG CATCAAATAG ACGATCTTCC TTTAATTTTG AATGCCTCCG CCGACAGAGT 
AGTCAAGATG ATATACCACT CTCTCCAAAT TTCCATCATC GCACTGCTTT ACCACTTCAC 
CTAATGCAAC AGCAGGTCAT GGCTGTTGCA GGTCTGGATT CCAGTAAAGC TCATAAACAT 
TCACCAAGTC GTTCCACGCG TTCATGGGCT ACCCCACCTG CAACCCCTCC CAACAGGGAC 
CATACCCCAT ACTATACACC TCTAATCCAA GTTGACAGGG CTGAATCTAC AGAACATATG 
AATGGTAGCC TGCCTTCCTT GCACAGGAGC TCCTGGTACA CAGACGACCC TGATATCTCC 
TACCGAACAT TTACACCAGC CAATTTAACA GTACCTAATG ACTTTAGGCA TAAACACAGT 
GACAAACAGA GGAGTGCAGA CAGTTTGGTA GAAGCGGTAC TTATATCTGA AGGCCTAGGA 
AGGTATGCAA AGGACCCTAA ATTTGTTTCA GCTACAAAAC ATGAGATTGC TGATGCTTGT 
GACATGACTA TCGATGAGAT GGAAAGTGCA GCAAGTAATC TTCTCAATGG AAATATTAGC 
AATGGGACCA ATGGGGATAT GTTTCCCATT TTAAGCAGAC AAGATTATGA ACTTCAAGAT 
TTTGGTCCTG GCTACAGTGA TGAAGAACCA GAACCAGGAC GATATGAAGA AGACTTAGCT 
GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010195.1
CDS	335..7024
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154427.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154427.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGG 
TCTGACTCTG GTGATGGCCG TCGACCAACT ATTTGCCGTG AAGAACGTGA CATTCGAGAC 
TATTGCAATG ATGATCATTA TTTGGGAGAG CAGGAATATT ATAGTGGAGA AGAATACTAT 
GAGGAAGACA GCATGTTGTC AGGAAACAGA CATGTGTATG ATTACCACTG TAGACATCAC 
TGCCATGACT CAGATTTTGA GAGACCAAAA GGTTACCATC ACCCACATGG GTTTTTTGAG 
GAAGATGATT CACAGACCTG TTATGATACG AAAAGATCTC CTAGGAGACG GCTGCTACCT 
CCAACACCAG CATCAAATAG ACGATCTTCC TTTAATTTTG AATGCCTCCG CCGACAGAGT 
AGTCAAGATG ATATACCACT CTCTCCAAAT TTCCATCATC GCACTGCTTT ACCACTTCAC 
CTAATGCAAC AGCAGGTCAT GGCTGTTGCA GGTCTGGATT CCAGTAAAGC TCATAAACAT 
TCACCAAGTC GTTCCACGCG TTCATGGGCT ACCCCACCTG CAACCCCTCC CAACAGGGAC 
CATACCCCAT ACTATACACC TCTAATCCAA GTTGACAGGG CTGAATCTAC AGAACATATG 
AATGGTAGCC TGCCTTCCTT GCACAGGAGC TCCTGGTACA CAGACGACCC TGATATCTCC 
TACCGAACAT TTACACCAGC CAATTTAACA GTACCTAATG ACTTTAGGCA TAAACACAGT 
GACAAACAGA GGAGTGCAGA CAGTTTGGTA GAAGCGGTAC TTATATCTGA AGGCCTAGGA 
AGGTATGCAA AGGACCCTAA ATTTGTTTCA GCTACAAAAC ATGAGATTGC TGATGCTTGT 
GACATGACTA TCGATGAGAT GGAAAGTGCA GCAAGTAATC TTCTCAATGG AAATATTAGC 
AATGGGACCA ATGGGGATAT GTTTCCCATT TTAAGCAGAC AAGATTATGA ACTTCAAGAT 
TTTGGTCCTG GCTACAGTGA TGAAGAACCA GAACCAGGAC GATATGAAGA AGACTTAGCT 
GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_025154428.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010196.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

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CloneID	OGa143886
Clone ID Related Accession (Same CDS sequence)	XM_025154428.1
Accession Version	XM_025154428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010196.1
CDS	335..7021
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154428.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154428.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_025154429.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010197.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

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CloneID	OGa143887
Clone ID Related Accession (Same CDS sequence)	XM_025154429.1
Accession Version	XM_025154429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATAAC TCCGAAGACA GCGCAAGAAT CTCCATCACT 
TTTTTCCGTC TTTTCCGAGT GATGAGGTTG GTGAAGCTGC TTAGCAGAGG AGAAGGAATC 
AGAACTTTAT TGTGGACATT TATTAAGTCA TTTCAGGCTC TGCCTTATGT TGCCCTTCTG 
ATAGCTATGC TGTTCTTCAT CTATGCTGTC ATTGGAATGC AGGTATTTGG AAAAGTGGCC 
ATGAGAGACA ACAATCAAAT AAACAGAAAC AACAATTTTC AAACTTTCCC CCAAGCTGTG 
CTGCTTCTCT TCAGGTGTGC AACTGGAGAA GCCTGGCAGG AAATCATGCT GGCATGTTTG 
CCTGGAAAAC GCTGTGATCC AGAATCTGAT TATAATCCAG GGGAAGAATA CACATGTGGA 
AGCAATTTTG CCATCATTTA TTTTATCAGT TTTTATATGC TTTGTGCATT TTTGATAATA 
AACTTATTTG TGGCTGTCAT TATGGATAAC TTTGACTATT TGACACGTGA CTGGTCCATT 
CTGGGTCCTC ATCATTTGGA TGAGTTCAAA AGGATATGGT CAGAATATGA CCCTGAAGCA 
AAGGGAAGGA TAAAGCATCT TGATGTGGTT ACGTTACTGA GACGCATTCA GCCACCACTT 
GGTTTTGGCA AATTATGCCC TCACCGGGTG GCCTGCAAGA GGTTAGTGGC CATGAATATG 
CCACTAAATA GTGACGGAAC CGTCATGTTC AATGCTACTT TGTTTGCTTT GGTTAGGACT 
GCTCTAAAGA TAAAAACAGA AGGTAACCTA GAGCAAGCAA ATGAAGAACT TAGAGCAGTT 
ATAAAGAAAA TATGGAAGAA AACTAGCATG AAATTACTTG ACCAAGTTGT CCCTCCGGCT 
GGCGATGATG AGGTAACCGT GGGGAAGTTC TATGCCACCT TCCTGATACA GGACTACTTT 
AGGAAATTCA AGAAACGCAA AGAACAAGGA CTGGTGGGGA AATATCCTGC GAAGAATACC 
ACGATTGCTC TGCAGGCAGG ACTAAGGACA CTTCATGATA TTGGCCCTGA AATACGCCGA 
GCTATATCCT GTGACTTGCA AGATGATGAA CCAGAGGAAA ACAATCCAGA CGAGGAGGAG 
GAAGTTTATA AAAGGAATGG TGCCCTCTTT GGCAACCATA TAAACCATAT TAGCAGTGAC 
AGACGAGATT CATTTCAGCA GATCAACACC ACGCACCGTC CCTTACATGT GCAGCGGCCT 
TCAATTCCAT CTGCAAGTGA TACTGAGAAA AATATATATC CTCATACAGG AAATTCTGTG 
TACCACAATC ATCATAACCA CAACTCAGTA GGAAAACAAG TTCCAAACTC AACAAATGCC 
AATCTCAATA ATGCCAACGT ATCCAAAGTT GTTCACGGAA AACACGCTAA TTTTGGAAGT 
CACGAGCATA GATCTGAAAA TGGTTACCAT TCGTACTCTA GAGCTGACCA TGAGAAGCGT 
AGAAGGCCAA GCAGTAGGAG AACCCGCTAC TACGAAACAT ATATTAGGTC TGACTCTGGT 
GATGGCCGTC GACCAACTAT TTGCCGTGAA GAACGTGACA TTCGAGACTA TTGCAATGAT 
GATCATTATT TGGGAGAGCA GGAATATTAT AGTGGAGAAG AATACTATGA GGAAGACAGC 
ATGTTGTCAG GAAACAGACA TGTGTATGAT TACCACTGTA GACATCACTG CCATGACTCA 
GATTTTGAGA GACCAAAAGG TTACCATCAC CCACATGGGT TTTTTGAGGA AGATGATTCA 
CAGACCTGTT ATGATACGAA AAGATCTCCT AGGAGACGGC TGCTACCTCC AACACCAGCA 
TCAAATAGAC GATCTTCCTT TAATTTTGAA TGCCTCCGCC GACAGAGTAG TCAAGATGAT 
ATACCACTCT CTCCAAATTT CCATCATCGC ACTGCTTTAC CACTTCACCT AATGCAACAG 
CAGGTCATGG CTGTTGCAGG TCTGGATTCC AGTAAAGCTC ATAAACATTC ACCAAGTCGT 
TCCACGCGTT CATGGGCTAC CCCACCTGCA ACCCCTCCCA ACAGGGACCA TACCCCATAC 
TATACACCTC TAATCCAAGT TGACAGGGCT GAATCTACAG AACATATGAA TGGTAGCCTG 
CCTTCCTTGC ACAGGAGCTC CTGGTACACA GACGACCCTG ATATCTCCTA CCGAACATTT 
ACACCAGCCA ATTTAACAGT ACCTAATGAC TTTAGGCATA AACACAGTGA CAAACAGAGG 
AGTGCAGACA GTTTGGTAGA AGCGGTACTT ATATCTGAAG GCCTAGGAAG GTATGCAAAG 
GACCCTAAAT TTGTTTCAGC TACAAAACAT GAGATTGCTG ATGCTTGTGA CATGACTATC 
GATGAGATGG AAAGTGCAGC AAGTAATCTT CTCAATGGAA ATATTAGCAA TGGGACCAAT 
GGGGATATGT TTCCCATTTT AAGCAGACAA GATTATGAAC TTCAAGATTT TGGTCCTGGC 
TACAGTGATG AAGAACCAGA ACCAGGACGA TATGAAGAAG ACTTAGCTGA TGAAATGATA 
TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010197.1
CDS	335..7012
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154429.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154429.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATAAC TCCGAAGACA GCGCAAGAAT CTCCATCACT 
TTTTTCCGTC TTTTCCGAGT GATGAGGTTG GTGAAGCTGC TTAGCAGAGG AGAAGGAATC 
AGAACTTTAT TGTGGACATT TATTAAGTCA TTTCAGGCTC TGCCTTATGT TGCCCTTCTG 
ATAGCTATGC TGTTCTTCAT CTATGCTGTC ATTGGAATGC AGGTATTTGG AAAAGTGGCC 
ATGAGAGACA ACAATCAAAT AAACAGAAAC AACAATTTTC AAACTTTCCC CCAAGCTGTG 
CTGCTTCTCT TCAGGTGTGC AACTGGAGAA GCCTGGCAGG AAATCATGCT GGCATGTTTG 
CCTGGAAAAC GCTGTGATCC AGAATCTGAT TATAATCCAG GGGAAGAATA CACATGTGGA 
AGCAATTTTG CCATCATTTA TTTTATCAGT TTTTATATGC TTTGTGCATT TTTGATAATA 
AACTTATTTG TGGCTGTCAT TATGGATAAC TTTGACTATT TGACACGTGA CTGGTCCATT 
CTGGGTCCTC ATCATTTGGA TGAGTTCAAA AGGATATGGT CAGAATATGA CCCTGAAGCA 
AAGGGAAGGA TAAAGCATCT TGATGTGGTT ACGTTACTGA GACGCATTCA GCCACCACTT 
GGTTTTGGCA AATTATGCCC TCACCGGGTG GCCTGCAAGA GGTTAGTGGC CATGAATATG 
CCACTAAATA GTGACGGAAC CGTCATGTTC AATGCTACTT TGTTTGCTTT GGTTAGGACT 
GCTCTAAAGA TAAAAACAGA AGGTAACCTA GAGCAAGCAA ATGAAGAACT TAGAGCAGTT 
ATAAAGAAAA TATGGAAGAA AACTAGCATG AAATTACTTG ACCAAGTTGT CCCTCCGGCT 
GGCGATGATG AGGTAACCGT GGGGAAGTTC TATGCCACCT TCCTGATACA GGACTACTTT 
AGGAAATTCA AGAAACGCAA AGAACAAGGA CTGGTGGGGA AATATCCTGC GAAGAATACC 
ACGATTGCTC TGCAGGCAGG ACTAAGGACA CTTCATGATA TTGGCCCTGA AATACGCCGA 
GCTATATCCT GTGACTTGCA AGATGATGAA CCAGAGGAAA ACAATCCAGA CGAGGAGGAG 
GAAGTTTATA AAAGGAATGG TGCCCTCTTT GGCAACCATA TAAACCATAT TAGCAGTGAC 
AGACGAGATT CATTTCAGCA GATCAACACC ACGCACCGTC CCTTACATGT GCAGCGGCCT 
TCAATTCCAT CTGCAAGTGA TACTGAGAAA AATATATATC CTCATACAGG AAATTCTGTG 
TACCACAATC ATCATAACCA CAACTCAGTA GGAAAACAAG TTCCAAACTC AACAAATGCC 
AATCTCAATA ATGCCAACGT ATCCAAAGTT GTTCACGGAA AACACGCTAA TTTTGGAAGT 
CACGAGCATA GATCTGAAAA TGGTTACCAT TCGTACTCTA GAGCTGACCA TGAGAAGCGT 
AGAAGGCCAA GCAGTAGGAG AACCCGCTAC TACGAAACAT ATATTAGGTC TGACTCTGGT 
GATGGCCGTC GACCAACTAT TTGCCGTGAA GAACGTGACA TTCGAGACTA TTGCAATGAT 
GATCATTATT TGGGAGAGCA GGAATATTAT AGTGGAGAAG AATACTATGA GGAAGACAGC 
ATGTTGTCAG GAAACAGACA TGTGTATGAT TACCACTGTA GACATCACTG CCATGACTCA 
GATTTTGAGA GACCAAAAGG TTACCATCAC CCACATGGGT TTTTTGAGGA AGATGATTCA 
CAGACCTGTT ATGATACGAA AAGATCTCCT AGGAGACGGC TGCTACCTCC AACACCAGCA 
TCAAATAGAC GATCTTCCTT TAATTTTGAA TGCCTCCGCC GACAGAGTAG TCAAGATGAT 
ATACCACTCT CTCCAAATTT CCATCATCGC ACTGCTTTAC CACTTCACCT AATGCAACAG 
CAGGTCATGG CTGTTGCAGG TCTGGATTCC AGTAAAGCTC ATAAACATTC ACCAAGTCGT 
TCCACGCGTT CATGGGCTAC CCCACCTGCA ACCCCTCCCA ACAGGGACCA TACCCCATAC 
TATACACCTC TAATCCAAGT TGACAGGGCT GAATCTACAG AACATATGAA TGGTAGCCTG 
CCTTCCTTGC ACAGGAGCTC CTGGTACACA GACGACCCTG ATATCTCCTA CCGAACATTT 
ACACCAGCCA ATTTAACAGT ACCTAATGAC TTTAGGCATA AACACAGTGA CAAACAGAGG 
AGTGCAGACA GTTTGGTAGA AGCGGTACTT ATATCTGAAG GCCTAGGAAG GTATGCAAAG 
GACCCTAAAT TTGTTTCAGC TACAAAACAT GAGATTGCTG ATGCTTGTGA CATGACTATC 
GATGAGATGG AAAGTGCAGC AAGTAATCTT CTCAATGGAA ATATTAGCAA TGGGACCAAT 
GGGGATATGT TTCCCATTTT AAGCAGACAA GATTATGAAC TTCAAGATTT TGGTCCTGGC 
TACAGTGATG AAGAACCAGA ACCAGGACGA TATGAAGAAG ACTTAGCTGA TGAAATGATA 
TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_025154430.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010198.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.

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CloneID	OGa143888
Clone ID Related Accession (Same CDS sequence)	XM_025154430.1
Accession Version	XM_025154430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010198.1
CDS	335..6937
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154430.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154430.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_025154431.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010199.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X26, mRNA.

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CloneID	OGa143889
Clone ID Related Accession (Same CDS sequence)	XM_025154431.1
Accession Version	XM_025154431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA ACTCCGAAGA CAGCGCAAGA ATCTCCATCA CTTTTTTCCG TCTTTTCCGA 
GTGATGAGGT TGGTGAAGCT GCTTAGCAGA GGAGAAGGAA TCAGAACTTT ATTGTGGACA 
TTTATTAAGT CATTTCAGGC TCTGCCTTAT GTTGCCCTTC TGATAGCTAT GCTGTTCTTC 
ATCTATGCTG TCATTGGAAT GCAGGTATTT GGAAAAGTGG CCATGAGAGA CAACAATCAA 
ATAAACAGAA ACAACAATTT TCAAACTTTC CCCCAAGCTG TGCTGCTTCT CTTCAGGTGT 
GCAACTGGAG AAGCCTGGCA GGAAATCATG CTGGCATGTT TGCCTGGAAA ACGCTGTGAT 
CCAGAATCTG ATTATAATCC AGGGGAAGAA TACACATGTG GAAGCAATTT TGCCATCATT 
TATTTTATCA GTTTTTATAT GCTTTGTGCA TTTTTGATAA TAAACTTATT TGTGGCTGTC 
ATTATGGATA ACTTTGACTA TTTGACACGT GACTGGTCCA TTCTGGGTCC TCATCATTTG 
GATGAGTTCA AAAGGATATG GTCAGAATAT GACCCTGAAG CAAAGGGAAG GATAAAGCAT 
CTTGATGTGG TTACGTTACT GAGACGCATT CAGCCACCAC TTGGTTTTGG CAAATTATGC 
CCTCACCGGG TGGCCTGCAA GAGGTTAGTG GCCATGAATA TGCCACTAAA TAGTGACGGA 
ACCGTCATGT TCAATGCTAC TTTGTTTGCT TTGGTTAGGA CTGCTCTAAA GATAAAAACA 
GAAGGTAACC TAGAGCAAGC AAATGAAGAA CTTAGAGCAG TTATAAAGAA AATATGGAAG 
AAAACTAGCA TGAAATTACT TGACCAAGTT GTCCCTCCGG CTGGCGATGA TGAGGTAACC 
GTGGGGAAGT TCTATGCCAC CTTCCTGATA CAGGACTACT TTAGGAAATT CAAGAAACGC 
AAAGAACAAG GACTGGTGGG GAAATATCCT GCGAAGAATA CCACGATTGC TCTGCAGGCA 
GGACTAAGGA CACTTCATGA TATTGGCCCT GAAATACGCC GAGCTATATC CTGTGACTTG 
CAAGATGATG AACCAGAGGA AAACAATCCA GACGAGGAGG AGGAAGTTTA TAAAAGGAAT 
GGTGCCCTCT TTGGCAACCA TATAAACCAT ATTAGCAGTG ACAGACGAGA TTCATTTCAG 
CAGATCAACA CCACGCACCG TCCCTTACAT GTGCAGCGGC CTTCAATTCC ATCTGCAAGT 
GATACTGAGA AAAATATATA TCCTCATACA GGAAATTCTG TGTACCACAA TCATCATAAC 
CACAACTCAG TAGGAAAACA AGTTCCAAAC TCAACAAATG CCAATCTCAA TAATGCCAAC 
GTATCCAAAG TTGTTCACGG AAAACACGCT AATTTTGGAA GTCACGAGCA TAGATCTGAA 
AATGGTTACC ATTCGTACTC TAGAGCTGAC CATGAGAAGC GTAGAAGGCC AAGCAGTAGG 
AGAACCCGCT ACTACGAAAC ATATATTAGG TCTGACTCTG GTGATGGCCG TCGACCAACT 
ATTTGCCGTG AAGAACGTGA CATTCGAGAC TATTGCAATG ATGATCATTA TTTGGGAGAG 
CAGGAATATT ATAGTGGAGA AGAATACTAT GAGGAAGACA GCATGTTGTC AGGAAACAGA 
CATGTGTATG ATTACCACTG TAGACATCAC TGCCATGACT CAGATTTTGA GAGACCAAAA 
GGTTACCATC ACCCACATGG GTTTTTTGAG GAAGATGATT CACAGACCTG TTATGATACG 
AAAAGATCTC CTAGGAGACG GCTGCTACCT CCAACACCAG CATCAAATAG ACGATCTTCC 
TTTAATTTTG AATGCCTCCG CCGACAGAGT AGTCAAGATG ATATACCACT CTCTCCAAAT 
TTCCATCATC GCACTGCTTT ACCACTTCAC CTAATGCAAC AGCAGGTCAT GGCTGTTGCA 
GGTCTGGATT CCAGTAAAGC TCATAAACAT TCACCAAGTC GTTCCACGCG TTCATGGGCT 
ACCCCACCTG CAACCCCTCC CAACAGGGAC CATACCCCAT ACTATACACC TCTAATCCAA 
GTTGACAGGG CTGAATCTAC AGAACATATG AATGGTAGCC TGCCTTCCTT GCACAGGAGC 
TCCTGGTACA CAGACGACCC TGATATCTCC TACCGAACAT TTACACCAGC CAATTTAACA 
GTACCTAATG ACTTTAGGCA TAAACACAGT GACAAACAGA GGAGTGCAGA CAGTTTGGTA 
GAAGCGGTAC TTATATCTGA AGGCCTAGGA AGGTATGCAA AGGACCCTAA ATTTGTTTCA 
GCTACAAAAC ATGAGATTGC TGATGCTTGT GACATGACTA TCGATGAGAT GGAAAGTGCA 
GCAAGTAATC TTCTCAATGG AAATATTAGC AATGGGACCA ATGGGGATAT GTTTCCCATT 
TTAAGCAGAC AAGATTATGA ACTTCAAGAT TTTGGTCCTG GCTACAGTGA TGAAGAACCA 
GAACCAGGAC GATATGAAGA AGACTTAGCT GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010199.1
CDS	335..6814
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154431.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154431.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA ACTCCGAAGA CAGCGCAAGA ATCTCCATCA CTTTTTTCCG TCTTTTCCGA 
GTGATGAGGT TGGTGAAGCT GCTTAGCAGA GGAGAAGGAA TCAGAACTTT ATTGTGGACA 
TTTATTAAGT CATTTCAGGC TCTGCCTTAT GTTGCCCTTC TGATAGCTAT GCTGTTCTTC 
ATCTATGCTG TCATTGGAAT GCAGGTATTT GGAAAAGTGG CCATGAGAGA CAACAATCAA 
ATAAACAGAA ACAACAATTT TCAAACTTTC CCCCAAGCTG TGCTGCTTCT CTTCAGGTGT 
GCAACTGGAG AAGCCTGGCA GGAAATCATG CTGGCATGTT TGCCTGGAAA ACGCTGTGAT 
CCAGAATCTG ATTATAATCC AGGGGAAGAA TACACATGTG GAAGCAATTT TGCCATCATT 
TATTTTATCA GTTTTTATAT GCTTTGTGCA TTTTTGATAA TAAACTTATT TGTGGCTGTC 
ATTATGGATA ACTTTGACTA TTTGACACGT GACTGGTCCA TTCTGGGTCC TCATCATTTG 
GATGAGTTCA AAAGGATATG GTCAGAATAT GACCCTGAAG CAAAGGGAAG GATAAAGCAT 
CTTGATGTGG TTACGTTACT GAGACGCATT CAGCCACCAC TTGGTTTTGG CAAATTATGC 
CCTCACCGGG TGGCCTGCAA GAGGTTAGTG GCCATGAATA TGCCACTAAA TAGTGACGGA 
ACCGTCATGT TCAATGCTAC TTTGTTTGCT TTGGTTAGGA CTGCTCTAAA GATAAAAACA 
GAAGGTAACC TAGAGCAAGC AAATGAAGAA CTTAGAGCAG TTATAAAGAA AATATGGAAG 
AAAACTAGCA TGAAATTACT TGACCAAGTT GTCCCTCCGG CTGGCGATGA TGAGGTAACC 
GTGGGGAAGT TCTATGCCAC CTTCCTGATA CAGGACTACT TTAGGAAATT CAAGAAACGC 
AAAGAACAAG GACTGGTGGG GAAATATCCT GCGAAGAATA CCACGATTGC TCTGCAGGCA 
GGACTAAGGA CACTTCATGA TATTGGCCCT GAAATACGCC GAGCTATATC CTGTGACTTG 
CAAGATGATG AACCAGAGGA AAACAATCCA GACGAGGAGG AGGAAGTTTA TAAAAGGAAT 
GGTGCCCTCT TTGGCAACCA TATAAACCAT ATTAGCAGTG ACAGACGAGA TTCATTTCAG 
CAGATCAACA CCACGCACCG TCCCTTACAT GTGCAGCGGC CTTCAATTCC ATCTGCAAGT 
GATACTGAGA AAAATATATA TCCTCATACA GGAAATTCTG TGTACCACAA TCATCATAAC 
CACAACTCAG TAGGAAAACA AGTTCCAAAC TCAACAAATG CCAATCTCAA TAATGCCAAC 
GTATCCAAAG TTGTTCACGG AAAACACGCT AATTTTGGAA GTCACGAGCA TAGATCTGAA 
AATGGTTACC ATTCGTACTC TAGAGCTGAC CATGAGAAGC GTAGAAGGCC AAGCAGTAGG 
AGAACCCGCT ACTACGAAAC ATATATTAGG TCTGACTCTG GTGATGGCCG TCGACCAACT 
ATTTGCCGTG AAGAACGTGA CATTCGAGAC TATTGCAATG ATGATCATTA TTTGGGAGAG 
CAGGAATATT ATAGTGGAGA AGAATACTAT GAGGAAGACA GCATGTTGTC AGGAAACAGA 
CATGTGTATG ATTACCACTG TAGACATCAC TGCCATGACT CAGATTTTGA GAGACCAAAA 
GGTTACCATC ACCCACATGG GTTTTTTGAG GAAGATGATT CACAGACCTG TTATGATACG 
AAAAGATCTC CTAGGAGACG GCTGCTACCT CCAACACCAG CATCAAATAG ACGATCTTCC 
TTTAATTTTG AATGCCTCCG CCGACAGAGT AGTCAAGATG ATATACCACT CTCTCCAAAT 
TTCCATCATC GCACTGCTTT ACCACTTCAC CTAATGCAAC AGCAGGTCAT GGCTGTTGCA 
GGTCTGGATT CCAGTAAAGC TCATAAACAT TCACCAAGTC GTTCCACGCG TTCATGGGCT 
ACCCCACCTG CAACCCCTCC CAACAGGGAC CATACCCCAT ACTATACACC TCTAATCCAA 
GTTGACAGGG CTGAATCTAC AGAACATATG AATGGTAGCC TGCCTTCCTT GCACAGGAGC 
TCCTGGTACA CAGACGACCC TGATATCTCC TACCGAACAT TTACACCAGC CAATTTAACA 
GTACCTAATG ACTTTAGGCA TAAACACAGT GACAAACAGA GGAGTGCAGA CAGTTTGGTA 
GAAGCGGTAC TTATATCTGA AGGCCTAGGA AGGTATGCAA AGGACCCTAA ATTTGTTTCA 
GCTACAAAAC ATGAGATTGC TGATGCTTGT GACATGACTA TCGATGAGAT GGAAAGTGCA 
GCAAGTAATC TTCTCAATGG AAATATTAGC AATGGGACCA ATGGGGATAT GTTTCCCATT 
TTAAGCAGAC AAGATTATGA ACTTCAAGAT TTTGGTCCTG GCTACAGTGA TGAAGAACCA 
GAACCAGGAC GATATGAAGA AGACTTAGCT GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_025154433.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010201.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X30, mRNA.

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CloneID	OGa143891
Clone ID Related Accession (Same CDS sequence)	XM_025154433.1
Accession Version	XM_025154433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010201.1
CDS	335..6877
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_025154433.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154433.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292975.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148461.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.

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CloneID	OGa41333
Clone ID Related Accession (Same CDS sequence)	XM_015292975.1 , XM_015292975.2
Accession Version	XM_015292975.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148461.1
CDS	335..7051
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292975.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292975.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292975.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148461.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

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CloneID	OGa41333
Clone ID Related Accession (Same CDS sequence)	XM_015292975.1 , XM_015292975.2
Accession Version	XM_015292975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148461.1
CDS	335..7051
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292975.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292975.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292976.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148462.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.

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CloneID	OGa41334
Clone ID Related Accession (Same CDS sequence)	XM_015292976.1 , XM_015292976.2
Accession Version	XM_015292976.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148462.1
CDS	335..7051
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292976.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292976.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292976.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148462.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

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CloneID	OGa41334
Clone ID Related Accession (Same CDS sequence)	XM_015292976.1 , XM_015292976.2
Accession Version	XM_015292976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148462.1
CDS	335..7051
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292976.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292976.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292977.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148463.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

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CloneID	OGa41335
Clone ID Related Accession (Same CDS sequence)	XM_015292977.2 , XM_015292977.1
Accession Version	XM_015292977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292977.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148463.1
CDS	335..6991
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292977.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292977.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292977.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148463.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.

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CloneID	OGa41335
Clone ID Related Accession (Same CDS sequence)	XM_015292977.2 , XM_015292977.1
Accession Version	XM_015292977.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148463.1
CDS	335..6991
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292977.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292977.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292978.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148464.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.

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CloneID	OGa41336
Clone ID Related Accession (Same CDS sequence)	XM_015292978.1 , XM_015292978.2
Accession Version	XM_015292978.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148464.1
CDS	335..6991
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292978.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292978.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292978.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148464.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

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CloneID	OGa41336
Clone ID Related Accession (Same CDS sequence)	XM_015292978.1 , XM_015292978.2
Accession Version	XM_015292978.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292978.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148464.1
CDS	335..6991
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292978.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292978.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCACTATT TCACTGATGC ATGGAACACT TTTGATGCCT TAATTGTTGT TGGTAGCGTC 
GTTGATATTG CTATAACAGA AGTTAATCCA AAGCCAACTG AAACAGTCAC AACTGATGAG 
TCTGGGAACT CCGAAGACAG CGCAAGAATC TCCATCACTT TTTTCCGTCT TTTCCGAGTG 
ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA GAAGGAATCA GAACTTTATT GTGGACATTT 
ATTAAGTCAT TTCAGGCTCT GCCTTATGTT GCCCTTCTGA TAGCTATGCT GTTCTTCATC 
TATGCTGTCA TTGGAATGCA GGTATTTGGA AAAGTGGCCA TGAGAGACAA CAATCAAATA 
AACAGAAACA ACAATTTTCA AACTTTCCCC CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA 
ACTGGAGAAG CCTGGCAGGA AATCATGCTG GCATGTTTGC CTGGAAAACG CTGTGATCCA 
GAATCTGATT ATAATCCAGG GGAAGAATAC ACATGTGGAA GCAATTTTGC CATCATTTAT 
TTTATCAGTT TTTATATGCT TTGTGCATTT TTGATAATAA ACTTATTTGT GGCTGTCATT 
ATGGATAACT TTGACTATTT GACACGTGAC TGGTCCATTC TGGGTCCTCA TCATTTGGAT 
GAGTTCAAAA GGATATGGTC AGAATATGAC CCTGAAGCAA AGGGAAGGAT AAAGCATCTT 
GATGTGGTTA CGTTACTGAG ACGCATTCAG CCACCACTTG GTTTTGGCAA ATTATGCCCT 
CACCGGGTGG CCTGCAAGAG GTTAGTGGCC ATGAATATGC CACTAAATAG TGACGGAACC 
GTCATGTTCA ATGCTACTTT GTTTGCTTTG GTTAGGACTG CTCTAAAGAT AAAAACAGAA 
GGTAACCTAG AGCAAGCAAA TGAAGAACTT AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA 
ACTAGCATGA AATTACTTGA CCAAGTTGTC CCTCCGGCTG GCGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACCTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGCAAA 
GAACAAGGAC TGGTGGGGAA ATATCCTGCG AAGAATACCA CGATTGCTCT GCAGGCAGGA 
CTAAGGACAC TTCATGATAT TGGCCCTGAA ATACGCCGAG CTATATCCTG TGACTTGCAA 
GATGATGAAC CAGAGGAAAA CAATCCAGAC GAGGAGGAGG AAGTTTATAA AAGGAATGGT 
GCCCTCTTTG GCAACCATAT AAACCATATT AGCAGTGACA GACGAGATTC ATTTCAGCAG 
ATCAACACCA CGCACCGTCC CTTACATGTG CAGCGGCCTT CAATTCCATC TGCAAGTGAT 
ACTGAGAAAA ATATATATCC TCATACAGGA AATTCTGTGT ACCACAATCA TCATAACCAC 
AACTCAGTAG GAAAACAAGT TCCAAACTCA ACAAATGCCA ATCTCAATAA TGCCAACGTA 
TCCAAAGTTG TTCACGGAAA ACACGCTAAT TTTGGAAGTC ACGAGCATAG ATCTGAAAAT 
GGTTACCATT CGTACTCTAG AGCTGACCAT GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA 
ACCCGCTACT ACGAAACATA TATTAGGTCT GACTCTGGTG ATGGCCGTCG ACCAACTATT 
TGCCGTGAAG AACGTGACAT TCGAGACTAT TGCAATGATG ATCATTATTT GGGAGAGCAG 
GAATATTATA GTGGAGAAGA ATACTATGAG GAAGACAGCA TGTTGTCAGG AAACAGACAT 
GTGTATGATT ACCACTGTAG ACATCACTGC CATGACTCAG ATTTTGAGAG ACCAAAAGGT 
TACCATCACC CACATGGGTT TTTTGAGGAA GATGATTCAC AGACCTGTTA TGATACGAAA 
AGATCTCCTA GGAGACGGCT GCTACCTCCA ACACCAGCAT CAAATAGACG ATCTTCCTTT 
AATTTTGAAT GCCTCCGCCG ACAGAGTAGT CAAGATGATA TACCACTCTC TCCAAATTTC 
CATCATCGCA CTGCTTTACC ACTTCACCTA ATGCAACAGC AGGTCATGGC TGTTGCAGGT 
CTGGATTCCA GTAAAGCTCA TAAACATTCA CCAAGTCGTT CCACGCGTTC ATGGGCTACC 
CCACCTGCAA CCCCTCCCAA CAGGGACCAT ACCCCATACT ATACACCTCT AATCCAAGTT 
GACAGGGCTG AATCTACAGA ACATATGAAT GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC 
TGGTACACAG ACGACCCTGA TATCTCCTAC CGAACATTTA CACCAGCCAA TTTAACAGTA 
CCTAATGACT TTAGGCATAA ACACAGTGAC AAACAGAGGA GTGCAGACAG TTTGGTAGAA 
GCGGTACTTA TATCTGAAGG CCTAGGAAGG TATGCAAAGG ACCCTAAATT TGTTTCAGCT 
ACAAAACATG AGATTGCTGA TGCTTGTGAC ATGACTATCG ATGAGATGGA AAGTGCAGCA 
AGTAATCTTC TCAATGGAAA TATTAGCAAT GGGACCAATG GGGATATGTT TCCCATTTTA 
AGCAGACAAG ATTATGAACT TCAAGATTTT GGTCCTGGCT ACAGTGATGA AGAACCAGAA 
CCAGGACGAT ATGAAGAAGA CTTAGCTGAT GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292979.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148465.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.

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CloneID	OGa41337
Clone ID Related Accession (Same CDS sequence)	XM_015292979.1 , XM_015292979.2
Accession Version	XM_015292979.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148465.1
CDS	335..6973
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292979.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292979.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292979.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148465.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

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CloneID	OGa41337
Clone ID Related Accession (Same CDS sequence)	XM_015292979.1 , XM_015292979.2
Accession Version	XM_015292979.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148465.1
CDS	335..6973
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292979.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292979.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292980.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148466.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

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CloneID	OGa41338
Clone ID Related Accession (Same CDS sequence)	XM_015292980.2 , XM_015292980.1
Accession Version	XM_015292980.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292980.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148466.1
CDS	335..6973
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292980.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292980.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292980.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148466.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X6, mRNA.

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CloneID	OGa41338
Clone ID Related Accession (Same CDS sequence)	XM_015292980.2 , XM_015292980.1
Accession Version	XM_015292980.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148466.1
CDS	335..6973
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292980.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292980.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GATTTTTGTA 
AGAAAAAAGG AAAGATGGCT AGGCTATTTT AGTGATGCCT GGAATACGTT TGACTCCCTC 
ATCGTTATTG GCAGCATAGT AGACGTCGTT CTCAGTGAAG CTGATCACTA TTTCACTGAT 
GCATGGAACA CTTTTGATGC CTTAATTGTT GTTGGTAGCG TCGTTGATAT TGCTATAACA 
GAAGTTAATC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292981.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148467.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.

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CloneID	OGa41339
Clone ID Related Accession (Same CDS sequence)	XM_015292981.2 , XM_015292981.1
Accession Version	XM_015292981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCCAAAGC CAACTGAAAC AGTCACAACT GATGAGTCTG GGAACTCCGA AGACAGCGCA 
AGAATCTCCA TCACTTTTTT CCGTCTTTTC CGAGTGATGA GGTTGGTGAA GCTGCTTAGC 
AGAGGAGAAG GAATCAGAAC TTTATTGTGG ACATTTATTA AGTCATTTCA GGCTCTGCCT 
TATGTTGCCC TTCTGATAGC TATGCTGTTC TTCATCTATG CTGTCATTGG AATGCAGGTA 
TTTGGAAAAG TGGCCATGAG AGACAACAAT CAAATAAACA GAAACAACAA TTTTCAAACT 
TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG TGTGCAACTG GAGAAGCCTG GCAGGAAATC 
ATGCTGGCAT GTTTGCCTGG AAAACGCTGT GATCCAGAAT CTGATTATAA TCCAGGGGAA 
GAATACACAT GTGGAAGCAA TTTTGCCATC ATTTATTTTA TCAGTTTTTA TATGCTTTGT 
GCATTTTTGA TAATAAACTT ATTTGTGGCT GTCATTATGG ATAACTTTGA CTATTTGACA 
CGTGACTGGT CCATTCTGGG TCCTCATCAT TTGGATGAGT TCAAAAGGAT ATGGTCAGAA 
TATGACCCTG AAGCAAAGGG AAGGATAAAG CATCTTGATG TGGTTACGTT ACTGAGACGC 
ATTCAGCCAC CACTTGGTTT TGGCAAATTA TGCCCTCACC GGGTGGCCTG CAAGAGGTTA 
GTGGCCATGA ATATGCCACT AAATAGTGAC GGAACCGTCA TGTTCAATGC TACTTTGTTT 
GCTTTGGTTA GGACTGCTCT AAAGATAAAA ACAGAAGGTA ACCTAGAGCA AGCAAATGAA 
GAACTTAGAG CAGTTATAAA GAAAATATGG AAGAAAACTA GCATGAAATT ACTTGACCAA 
GTTGTCCCTC CGGCTGGCGA TGATGAGGTA ACCGTGGGGA AGTTCTATGC CACCTTCCTG 
ATACAGGACT ACTTTAGGAA ATTCAAGAAA CGCAAAGAAC AAGGACTGGT GGGGAAATAT 
CCTGCGAAGA ATACCACGAT TGCTCTGCAG GCAGGACTAA GGACACTTCA TGATATTGGC 
CCTGAAATAC GCCGAGCTAT ATCCTGTGAC TTGCAAGATG ATGAACCAGA GGAAAACAAT 
CCAGACGAGG AGGAGGAAGT TTATAAAAGG AATGGTGCCC TCTTTGGCAA CCATATAAAC 
CATATTAGCA GTGACAGACG AGATTCATTT CAGCAGATCA ACACCACGCA CCGTCCCTTA 
CATGTGCAGC GGCCTTCAAT TCCATCTGCA AGTGATACTG AGAAAAATAT ATATCCTCAT 
ACAGGAAATT CTGTGTACCA CAATCATCAT AACCACAACT CAGTAGGAAA ACAAGTTCCA 
AACTCAACAA ATGCCAATCT CAATAATGCC AACGTATCCA AAGTTGTTCA CGGAAAACAC 
GCTAATTTTG GAAGTCACGA GCATAGATCT GAAAATGGTT ACCATTCGTA CTCTAGAGCT 
GACCATGAGA AGCGTAGAAG GCCAAGCAGT AGGAGAACCC GCTACTACGA AACATATATT 
AGGTCTGACT CTGGTGATGG CCGTCGACCA ACTATTTGCC GTGAAGAACG TGACATTCGA 
GACTATTGCA ATGATGATCA TTATTTGGGA GAGCAGGAAT ATTATAGTGG AGAAGAATAC 
TATGAGGAAG ACAGCATGTT GTCAGGAAAC AGACATGTGT ATGATTACCA CTGTAGACAT 
CACTGCCATG ACTCAGATTT TGAGAGACCA AAAGGTTACC ATCACCCACA TGGGTTTTTT 
GAGGAAGATG ATTCACAGAC CTGTTATGAT ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA 
CCTCCAACAC CAGCATCAAA TAGACGATCT TCCTTTAATT TTGAATGCCT CCGCCGACAG 
AGTAGTCAAG ATGATATACC ACTCTCTCCA AATTTCCATC ATCGCACTGC TTTACCACTT 
CACCTAATGC AACAGCAGGT CATGGCTGTT GCAGGTCTGG ATTCCAGTAA AGCTCATAAA 
CATTCACCAA GTCGTTCCAC GCGTTCATGG GCTACCCCAC CTGCAACCCC TCCCAACAGG 
GACCATACCC CATACTATAC ACCTCTAATC CAAGTTGACA GGGCTGAATC TACAGAACAT 
ATGAATGGTA GCCTGCCTTC CTTGCACAGG AGCTCCTGGT ACACAGACGA CCCTGATATC 
TCCTACCGAA CATTTACACC AGCCAATTTA ACAGTACCTA ATGACTTTAG GCATAAACAC 
AGTGACAAAC AGAGGAGTGC AGACAGTTTG GTAGAAGCGG TACTTATATC TGAAGGCCTA 
GGAAGGTATG CAAAGGACCC TAAATTTGTT TCAGCTACAA AACATGAGAT TGCTGATGCT 
TGTGACATGA CTATCGATGA GATGGAAAGT GCAGCAAGTA ATCTTCTCAA TGGAAATATT 
AGCAATGGGA CCAATGGGGA TATGTTTCCC ATTTTAAGCA GACAAGATTA TGAACTTCAA 
GATTTTGGTC CTGGCTACAG TGATGAAGAA CCAGAACCAG GACGATATGA AGAAGACTTA 
GCTGATGAAA TGATATGCAT TACAAGCTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148467.1
CDS	335..6967
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292981.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292981.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCCAAAGC CAACTGAAAC AGTCACAACT GATGAGTCTG GGAACTCCGA AGACAGCGCA 
AGAATCTCCA TCACTTTTTT CCGTCTTTTC CGAGTGATGA GGTTGGTGAA GCTGCTTAGC 
AGAGGAGAAG GAATCAGAAC TTTATTGTGG ACATTTATTA AGTCATTTCA GGCTCTGCCT 
TATGTTGCCC TTCTGATAGC TATGCTGTTC TTCATCTATG CTGTCATTGG AATGCAGGTA 
TTTGGAAAAG TGGCCATGAG AGACAACAAT CAAATAAACA GAAACAACAA TTTTCAAACT 
TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG TGTGCAACTG GAGAAGCCTG GCAGGAAATC 
ATGCTGGCAT GTTTGCCTGG AAAACGCTGT GATCCAGAAT CTGATTATAA TCCAGGGGAA 
GAATACACAT GTGGAAGCAA TTTTGCCATC ATTTATTTTA TCAGTTTTTA TATGCTTTGT 
GCATTTTTGA TAATAAACTT ATTTGTGGCT GTCATTATGG ATAACTTTGA CTATTTGACA 
CGTGACTGGT CCATTCTGGG TCCTCATCAT TTGGATGAGT TCAAAAGGAT ATGGTCAGAA 
TATGACCCTG AAGCAAAGGG AAGGATAAAG CATCTTGATG TGGTTACGTT ACTGAGACGC 
ATTCAGCCAC CACTTGGTTT TGGCAAATTA TGCCCTCACC GGGTGGCCTG CAAGAGGTTA 
GTGGCCATGA ATATGCCACT AAATAGTGAC GGAACCGTCA TGTTCAATGC TACTTTGTTT 
GCTTTGGTTA GGACTGCTCT AAAGATAAAA ACAGAAGGTA ACCTAGAGCA AGCAAATGAA 
GAACTTAGAG CAGTTATAAA GAAAATATGG AAGAAAACTA GCATGAAATT ACTTGACCAA 
GTTGTCCCTC CGGCTGGCGA TGATGAGGTA ACCGTGGGGA AGTTCTATGC CACCTTCCTG 
ATACAGGACT ACTTTAGGAA ATTCAAGAAA CGCAAAGAAC AAGGACTGGT GGGGAAATAT 
CCTGCGAAGA ATACCACGAT TGCTCTGCAG GCAGGACTAA GGACACTTCA TGATATTGGC 
CCTGAAATAC GCCGAGCTAT ATCCTGTGAC TTGCAAGATG ATGAACCAGA GGAAAACAAT 
CCAGACGAGG AGGAGGAAGT TTATAAAAGG AATGGTGCCC TCTTTGGCAA CCATATAAAC 
CATATTAGCA GTGACAGACG AGATTCATTT CAGCAGATCA ACACCACGCA CCGTCCCTTA 
CATGTGCAGC GGCCTTCAAT TCCATCTGCA AGTGATACTG AGAAAAATAT ATATCCTCAT 
ACAGGAAATT CTGTGTACCA CAATCATCAT AACCACAACT CAGTAGGAAA ACAAGTTCCA 
AACTCAACAA ATGCCAATCT CAATAATGCC AACGTATCCA AAGTTGTTCA CGGAAAACAC 
GCTAATTTTG GAAGTCACGA GCATAGATCT GAAAATGGTT ACCATTCGTA CTCTAGAGCT 
GACCATGAGA AGCGTAGAAG GCCAAGCAGT AGGAGAACCC GCTACTACGA AACATATATT 
AGGTCTGACT CTGGTGATGG CCGTCGACCA ACTATTTGCC GTGAAGAACG TGACATTCGA 
GACTATTGCA ATGATGATCA TTATTTGGGA GAGCAGGAAT ATTATAGTGG AGAAGAATAC 
TATGAGGAAG ACAGCATGTT GTCAGGAAAC AGACATGTGT ATGATTACCA CTGTAGACAT 
CACTGCCATG ACTCAGATTT TGAGAGACCA AAAGGTTACC ATCACCCACA TGGGTTTTTT 
GAGGAAGATG ATTCACAGAC CTGTTATGAT ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA 
CCTCCAACAC CAGCATCAAA TAGACGATCT TCCTTTAATT TTGAATGCCT CCGCCGACAG 
AGTAGTCAAG ATGATATACC ACTCTCTCCA AATTTCCATC ATCGCACTGC TTTACCACTT 
CACCTAATGC AACAGCAGGT CATGGCTGTT GCAGGTCTGG ATTCCAGTAA AGCTCATAAA 
CATTCACCAA GTCGTTCCAC GCGTTCATGG GCTACCCCAC CTGCAACCCC TCCCAACAGG 
GACCATACCC CATACTATAC ACCTCTAATC CAAGTTGACA GGGCTGAATC TACAGAACAT 
ATGAATGGTA GCCTGCCTTC CTTGCACAGG AGCTCCTGGT ACACAGACGA CCCTGATATC 
TCCTACCGAA CATTTACACC AGCCAATTTA ACAGTACCTA ATGACTTTAG GCATAAACAC 
AGTGACAAAC AGAGGAGTGC AGACAGTTTG GTAGAAGCGG TACTTATATC TGAAGGCCTA 
GGAAGGTATG CAAAGGACCC TAAATTTGTT TCAGCTACAA AACATGAGAT TGCTGATGCT 
TGTGACATGA CTATCGATGA GATGGAAAGT GCAGCAAGTA ATCTTCTCAA TGGAAATATT 
AGCAATGGGA CCAATGGGGA TATGTTTCCC ATTTTAAGCA GACAAGATTA TGAACTTCAA 
GATTTTGGTC CTGGCTACAG TGATGAAGAA CCAGAACCAG GACGATATGA AGAAGACTTA 
GCTGATGAAA TGATATGCAT TACAAGCTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292981.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148467.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.

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CloneID	OGa41339
Clone ID Related Accession (Same CDS sequence)	XM_015292981.2 , XM_015292981.1
Accession Version	XM_015292981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCCAAAGC CAACTGAAAC AGTCACAACT GATGAGTCTG GGAACTCCGA AGACAGCGCA 
AGAATCTCCA TCACTTTTTT CCGTCTTTTC CGAGTGATGA GGTTGGTGAA GCTGCTTAGC 
AGAGGAGAAG GAATCAGAAC TTTATTGTGG ACATTTATTA AGTCATTTCA GGCTCTGCCT 
TATGTTGCCC TTCTGATAGC TATGCTGTTC TTCATCTATG CTGTCATTGG AATGCAGGTA 
TTTGGAAAAG TGGCCATGAG AGACAACAAT CAAATAAACA GAAACAACAA TTTTCAAACT 
TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG TGTGCAACTG GAGAAGCCTG GCAGGAAATC 
ATGCTGGCAT GTTTGCCTGG AAAACGCTGT GATCCAGAAT CTGATTATAA TCCAGGGGAA 
GAATACACAT GTGGAAGCAA TTTTGCCATC ATTTATTTTA TCAGTTTTTA TATGCTTTGT 
GCATTTTTGA TAATAAACTT ATTTGTGGCT GTCATTATGG ATAACTTTGA CTATTTGACA 
CGTGACTGGT CCATTCTGGG TCCTCATCAT TTGGATGAGT TCAAAAGGAT ATGGTCAGAA 
TATGACCCTG AAGCAAAGGG AAGGATAAAG CATCTTGATG TGGTTACGTT ACTGAGACGC 
ATTCAGCCAC CACTTGGTTT TGGCAAATTA TGCCCTCACC GGGTGGCCTG CAAGAGGTTA 
GTGGCCATGA ATATGCCACT AAATAGTGAC GGAACCGTCA TGTTCAATGC TACTTTGTTT 
GCTTTGGTTA GGACTGCTCT AAAGATAAAA ACAGAAGGTA ACCTAGAGCA AGCAAATGAA 
GAACTTAGAG CAGTTATAAA GAAAATATGG AAGAAAACTA GCATGAAATT ACTTGACCAA 
GTTGTCCCTC CGGCTGGCGA TGATGAGGTA ACCGTGGGGA AGTTCTATGC CACCTTCCTG 
ATACAGGACT ACTTTAGGAA ATTCAAGAAA CGCAAAGAAC AAGGACTGGT GGGGAAATAT 
CCTGCGAAGA ATACCACGAT TGCTCTGCAG GCAGGACTAA GGACACTTCA TGATATTGGC 
CCTGAAATAC GCCGAGCTAT ATCCTGTGAC TTGCAAGATG ATGAACCAGA GGAAAACAAT 
CCAGACGAGG AGGAGGAAGT TTATAAAAGG AATGGTGCCC TCTTTGGCAA CCATATAAAC 
CATATTAGCA GTGACAGACG AGATTCATTT CAGCAGATCA ACACCACGCA CCGTCCCTTA 
CATGTGCAGC GGCCTTCAAT TCCATCTGCA AGTGATACTG AGAAAAATAT ATATCCTCAT 
ACAGGAAATT CTGTGTACCA CAATCATCAT AACCACAACT CAGTAGGAAA ACAAGTTCCA 
AACTCAACAA ATGCCAATCT CAATAATGCC AACGTATCCA AAGTTGTTCA CGGAAAACAC 
GCTAATTTTG GAAGTCACGA GCATAGATCT GAAAATGGTT ACCATTCGTA CTCTAGAGCT 
GACCATGAGA AGCGTAGAAG GCCAAGCAGT AGGAGAACCC GCTACTACGA AACATATATT 
AGGTCTGACT CTGGTGATGG CCGTCGACCA ACTATTTGCC GTGAAGAACG TGACATTCGA 
GACTATTGCA ATGATGATCA TTATTTGGGA GAGCAGGAAT ATTATAGTGG AGAAGAATAC 
TATGAGGAAG ACAGCATGTT GTCAGGAAAC AGACATGTGT ATGATTACCA CTGTAGACAT 
CACTGCCATG ACTCAGATTT TGAGAGACCA AAAGGTTACC ATCACCCACA TGGGTTTTTT 
GAGGAAGATG ATTCACAGAC CTGTTATGAT ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA 
CCTCCAACAC CAGCATCAAA TAGACGATCT TCCTTTAATT TTGAATGCCT CCGCCGACAG 
AGTAGTCAAG ATGATATACC ACTCTCTCCA AATTTCCATC ATCGCACTGC TTTACCACTT 
CACCTAATGC AACAGCAGGT CATGGCTGTT GCAGGTCTGG ATTCCAGTAA AGCTCATAAA 
CATTCACCAA GTCGTTCCAC GCGTTCATGG GCTACCCCAC CTGCAACCCC TCCCAACAGG 
GACCATACCC CATACTATAC ACCTCTAATC CAAGTTGACA GGGCTGAATC TACAGAACAT 
ATGAATGGTA GCCTGCCTTC CTTGCACAGG AGCTCCTGGT ACACAGACGA CCCTGATATC 
TCCTACCGAA CATTTACACC AGCCAATTTA ACAGTACCTA ATGACTTTAG GCATAAACAC 
AGTGACAAAC AGAGGAGTGC AGACAGTTTG GTAGAAGCGG TACTTATATC TGAAGGCCTA 
GGAAGGTATG CAAAGGACCC TAAATTTGTT TCAGCTACAA AACATGAGAT TGCTGATGCT 
TGTGACATGA CTATCGATGA GATGGAAAGT GCAGCAAGTA ATCTTCTCAA TGGAAATATT 
AGCAATGGGA CCAATGGGGA TATGTTTCCC ATTTTAAGCA GACAAGATTA TGAACTTCAA 
GATTTTGGTC CTGGCTACAG TGATGAAGAA CCAGAACCAG GACGATATGA AGAAGACTTA 
GCTGATGAAA TGATATGCAT TACAAGCTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148467.1
CDS	335..6967
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292981.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292981.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCCAAAGC CAACTGAAAC AGTCACAACT GATGAGTCTG GGAACTCCGA AGACAGCGCA 
AGAATCTCCA TCACTTTTTT CCGTCTTTTC CGAGTGATGA GGTTGGTGAA GCTGCTTAGC 
AGAGGAGAAG GAATCAGAAC TTTATTGTGG ACATTTATTA AGTCATTTCA GGCTCTGCCT 
TATGTTGCCC TTCTGATAGC TATGCTGTTC TTCATCTATG CTGTCATTGG AATGCAGGTA 
TTTGGAAAAG TGGCCATGAG AGACAACAAT CAAATAAACA GAAACAACAA TTTTCAAACT 
TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG TGTGCAACTG GAGAAGCCTG GCAGGAAATC 
ATGCTGGCAT GTTTGCCTGG AAAACGCTGT GATCCAGAAT CTGATTATAA TCCAGGGGAA 
GAATACACAT GTGGAAGCAA TTTTGCCATC ATTTATTTTA TCAGTTTTTA TATGCTTTGT 
GCATTTTTGA TAATAAACTT ATTTGTGGCT GTCATTATGG ATAACTTTGA CTATTTGACA 
CGTGACTGGT CCATTCTGGG TCCTCATCAT TTGGATGAGT TCAAAAGGAT ATGGTCAGAA 
TATGACCCTG AAGCAAAGGG AAGGATAAAG CATCTTGATG TGGTTACGTT ACTGAGACGC 
ATTCAGCCAC CACTTGGTTT TGGCAAATTA TGCCCTCACC GGGTGGCCTG CAAGAGGTTA 
GTGGCCATGA ATATGCCACT AAATAGTGAC GGAACCGTCA TGTTCAATGC TACTTTGTTT 
GCTTTGGTTA GGACTGCTCT AAAGATAAAA ACAGAAGGTA ACCTAGAGCA AGCAAATGAA 
GAACTTAGAG CAGTTATAAA GAAAATATGG AAGAAAACTA GCATGAAATT ACTTGACCAA 
GTTGTCCCTC CGGCTGGCGA TGATGAGGTA ACCGTGGGGA AGTTCTATGC CACCTTCCTG 
ATACAGGACT ACTTTAGGAA ATTCAAGAAA CGCAAAGAAC AAGGACTGGT GGGGAAATAT 
CCTGCGAAGA ATACCACGAT TGCTCTGCAG GCAGGACTAA GGACACTTCA TGATATTGGC 
CCTGAAATAC GCCGAGCTAT ATCCTGTGAC TTGCAAGATG ATGAACCAGA GGAAAACAAT 
CCAGACGAGG AGGAGGAAGT TTATAAAAGG AATGGTGCCC TCTTTGGCAA CCATATAAAC 
CATATTAGCA GTGACAGACG AGATTCATTT CAGCAGATCA ACACCACGCA CCGTCCCTTA 
CATGTGCAGC GGCCTTCAAT TCCATCTGCA AGTGATACTG AGAAAAATAT ATATCCTCAT 
ACAGGAAATT CTGTGTACCA CAATCATCAT AACCACAACT CAGTAGGAAA ACAAGTTCCA 
AACTCAACAA ATGCCAATCT CAATAATGCC AACGTATCCA AAGTTGTTCA CGGAAAACAC 
GCTAATTTTG GAAGTCACGA GCATAGATCT GAAAATGGTT ACCATTCGTA CTCTAGAGCT 
GACCATGAGA AGCGTAGAAG GCCAAGCAGT AGGAGAACCC GCTACTACGA AACATATATT 
AGGTCTGACT CTGGTGATGG CCGTCGACCA ACTATTTGCC GTGAAGAACG TGACATTCGA 
GACTATTGCA ATGATGATCA TTATTTGGGA GAGCAGGAAT ATTATAGTGG AGAAGAATAC 
TATGAGGAAG ACAGCATGTT GTCAGGAAAC AGACATGTGT ATGATTACCA CTGTAGACAT 
CACTGCCATG ACTCAGATTT TGAGAGACCA AAAGGTTACC ATCACCCACA TGGGTTTTTT 
GAGGAAGATG ATTCACAGAC CTGTTATGAT ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA 
CCTCCAACAC CAGCATCAAA TAGACGATCT TCCTTTAATT TTGAATGCCT CCGCCGACAG 
AGTAGTCAAG ATGATATACC ACTCTCTCCA AATTTCCATC ATCGCACTGC TTTACCACTT 
CACCTAATGC AACAGCAGGT CATGGCTGTT GCAGGTCTGG ATTCCAGTAA AGCTCATAAA 
CATTCACCAA GTCGTTCCAC GCGTTCATGG GCTACCCCAC CTGCAACCCC TCCCAACAGG 
GACCATACCC CATACTATAC ACCTCTAATC CAAGTTGACA GGGCTGAATC TACAGAACAT 
ATGAATGGTA GCCTGCCTTC CTTGCACAGG AGCTCCTGGT ACACAGACGA CCCTGATATC 
TCCTACCGAA CATTTACACC AGCCAATTTA ACAGTACCTA ATGACTTTAG GCATAAACAC 
AGTGACAAAC AGAGGAGTGC AGACAGTTTG GTAGAAGCGG TACTTATATC TGAAGGCCTA 
GGAAGGTATG CAAAGGACCC TAAATTTGTT TCAGCTACAA AACATGAGAT TGCTGATGCT 
TGTGACATGA CTATCGATGA GATGGAAAGT GCAGCAAGTA ATCTTCTCAA TGGAAATATT 
AGCAATGGGA CCAATGGGGA TATGTTTCCC ATTTTAAGCA GACAAGATTA TGAACTTCAA 
GATTTTGGTC CTGGCTACAG TGATGAAGAA CCAGAACCAG GACGATATGA AGAAGACTTA 
GCTGATGAAA TGATATGCAT TACAAGCTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292982.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148468.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.

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CloneID	OGa41340
Clone ID Related Accession (Same CDS sequence)	XM_015292982.1 , XM_015292982.2
Accession Version	XM_015292982.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148468.1
CDS	335..6961
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292982.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292982.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292982.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148468.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.

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CloneID	OGa41340
Clone ID Related Accession (Same CDS sequence)	XM_015292982.1 , XM_015292982.2
Accession Version	XM_015292982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292982.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148468.1
CDS	335..6961
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292982.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292982.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292983.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148469.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.

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CloneID	OGa41341
Clone ID Related Accession (Same CDS sequence)	XM_015292983.2 , XM_015292983.1
Accession Version	XM_015292983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292983.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148469.1
CDS	335..6961
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292983.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292983.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292983.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148469.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.

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CloneID	OGa41341
Clone ID Related Accession (Same CDS sequence)	XM_015292983.2 , XM_015292983.1
Accession Version	XM_015292983.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148469.1
CDS	335..6961
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292983.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292983.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATCCA 
AAGCCAACTG AAACAGTCAC AACTGATGAG TCTGGGAACT CCGAAGACAG CGCAAGAATC 
TCCATCACTT TTTTCCGTCT TTTCCGAGTG ATGAGGTTGG TGAAGCTGCT TAGCAGAGGA 
GAAGGAATCA GAACTTTATT GTGGACATTT ATTAAGTCAT TTCAGGCTCT GCCTTATGTT 
GCCCTTCTGA TAGCTATGCT GTTCTTCATC TATGCTGTCA TTGGAATGCA GGTATTTGGA 
AAAGTGGCCA TGAGAGACAA CAATCAAATA AACAGAAACA ACAATTTTCA AACTTTCCCC 
CAAGCTGTGC TGCTTCTCTT CAGGTGTGCA ACTGGAGAAG CCTGGCAGGA AATCATGCTG 
GCATGTTTGC CTGGAAAACG CTGTGATCCA GAATCTGATT ATAATCCAGG GGAAGAATAC 
ACATGTGGAA GCAATTTTGC CATCATTTAT TTTATCAGTT TTTATATGCT TTGTGCATTT 
TTGATAATAA ACTTATTTGT GGCTGTCATT ATGGATAACT TTGACTATTT GACACGTGAC 
TGGTCCATTC TGGGTCCTCA TCATTTGGAT GAGTTCAAAA GGATATGGTC AGAATATGAC 
CCTGAAGCAA AGGGAAGGAT AAAGCATCTT GATGTGGTTA CGTTACTGAG ACGCATTCAG 
CCACCACTTG GTTTTGGCAA ATTATGCCCT CACCGGGTGG CCTGCAAGAG GTTAGTGGCC 
ATGAATATGC CACTAAATAG TGACGGAACC GTCATGTTCA ATGCTACTTT GTTTGCTTTG 
GTTAGGACTG CTCTAAAGAT AAAAACAGAA GGTAACCTAG AGCAAGCAAA TGAAGAACTT 
AGAGCAGTTA TAAAGAAAAT ATGGAAGAAA ACTAGCATGA AATTACTTGA CCAAGTTGTC 
CCTCCGGCTG GCGATGATGA GGTAACCGTG GGGAAGTTCT ATGCCACCTT CCTGATACAG 
GACTACTTTA GGAAATTCAA GAAACGCAAA GAACAAGGAC TGGTGGGGAA ATATCCTGCG 
AAGAATACCA CGATTGCTCT GCAGGCAGGA CTAAGGACAC TTCATGATAT TGGCCCTGAA 
ATACGCCGAG CTATATCCTG TGACTTGCAA GATGATGAAC CAGAGGAAAA CAATCCAGAC 
GAGGAGGAGG AAGTTTATAA AAGGAATGGT GCCCTCTTTG GCAACCATAT AAACCATATT 
AGCAGTGACA GACGAGATTC ATTTCAGCAG ATCAACACCA CGCACCGTCC CTTACATGTG 
CAGCGGCCTT CAATTCCATC TGCAAGTGAT ACTGAGAAAA ATATATATCC TCATACAGGA 
AATTCTGTGT ACCACAATCA TCATAACCAC AACTCAGTAG GAAAACAAGT TCCAAACTCA 
ACAAATGCCA ATCTCAATAA TGCCAACGTA TCCAAAGTTG TTCACGGAAA ACACGCTAAT 
TTTGGAAGTC ACGAGCATAG ATCTGAAAAT GGTTACCATT CGTACTCTAG AGCTGACCAT 
GAGAAGCGTA GAAGGCCAAG CAGTAGGAGA ACCCGCTACT ACGAAACATA TATTAGGTCT 
GACTCTGGTG ATGGCCGTCG ACCAACTATT TGCCGTGAAG AACGTGACAT TCGAGACTAT 
TGCAATGATG ATCATTATTT GGGAGAGCAG GAATATTATA GTGGAGAAGA ATACTATGAG 
GAAGACAGCA TGTTGTCAGG AAACAGACAT GTGTATGATT ACCACTGTAG ACATCACTGC 
CATGACTCAG ATTTTGAGAG ACCAAAAGGT TACCATCACC CACATGGGTT TTTTGAGGAA 
GATGATTCAC AGACCTGTTA TGATACGAAA AGATCTCCTA GGAGACGGCT GCTACCTCCA 
ACACCAGCAT CAAATAGACG ATCTTCCTTT AATTTTGAAT GCCTCCGCCG ACAGAGTAGT 
CAAGATGATA TACCACTCTC TCCAAATTTC CATCATCGCA CTGCTTTACC ACTTCACCTA 
ATGCAACAGC AGGTCATGGC TGTTGCAGGT CTGGATTCCA GTAAAGCTCA TAAACATTCA 
CCAAGTCGTT CCACGCGTTC ATGGGCTACC CCACCTGCAA CCCCTCCCAA CAGGGACCAT 
ACCCCATACT ATACACCTCT AATCCAAGTT GACAGGGCTG AATCTACAGA ACATATGAAT 
GGTAGCCTGC CTTCCTTGCA CAGGAGCTCC TGGTACACAG ACGACCCTGA TATCTCCTAC 
CGAACATTTA CACCAGCCAA TTTAACAGTA CCTAATGACT TTAGGCATAA ACACAGTGAC 
AAACAGAGGA GTGCAGACAG TTTGGTAGAA GCGGTACTTA TATCTGAAGG CCTAGGAAGG 
TATGCAAAGG ACCCTAAATT TGTTTCAGCT ACAAAACATG AGATTGCTGA TGCTTGTGAC 
ATGACTATCG ATGAGATGGA AAGTGCAGCA AGTAATCTTC TCAATGGAAA TATTAGCAAT 
GGGACCAATG GGGATATGTT TCCCATTTTA AGCAGACAAG ATTATGAACT TCAAGATTTT 
GGTCCTGGCT ACAGTGATGA AGAACCAGAA CCAGGACGAT ATGAAGAAGA CTTAGCTGAT 
GAAATGATAT GCATTACAAG CTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292984.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148470.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X10, mRNA.

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CloneID	OGa41342
Clone ID Related Accession (Same CDS sequence)	XM_015292984.1 , XM_015292984.2
Accession Version	XM_015292984.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATAACTCCG AAGACAGCGC AAGAATCTCC ATCACTTTTT TCCGTCTTTT CCGAGTGATG 
AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA GGAATCAGAA CTTTATTGTG GACATTTATT 
AAGTCATTTC AGGCTCTGCC TTATGTTGCC CTTCTGATAG CTATGCTGTT CTTCATCTAT 
GCTGTCATTG GAATGCAGGT ATTTGGAAAA GTGGCCATGA GAGACAACAA TCAAATAAAC 
AGAAACAACA ATTTTCAAAC TTTCCCCCAA GCTGTGCTGC TTCTCTTCAG GTGTGCAACT 
GGAGAAGCCT GGCAGGAAAT CATGCTGGCA TGTTTGCCTG GAAAACGCTG TGATCCAGAA 
TCTGATTATA ATCCAGGGGA AGAATACACA TGTGGAAGCA ATTTTGCCAT CATTTATTTT 
ATCAGTTTTT ATATGCTTTG TGCATTTTTG ATAATAAACT TATTTGTGGC TGTCATTATG 
GATAACTTTG ACTATTTGAC ACGTGACTGG TCCATTCTGG GTCCTCATCA TTTGGATGAG 
TTCAAAAGGA TATGGTCAGA ATATGACCCT GAAGCAAAGG GAAGGATAAA GCATCTTGAT 
GTGGTTACGT TACTGAGACG CATTCAGCCA CCACTTGGTT TTGGCAAATT ATGCCCTCAC 
CGGGTGGCCT GCAAGAGGTT AGTGGCCATG AATATGCCAC TAAATAGTGA CGGAACCGTC 
ATGTTCAATG CTACTTTGTT TGCTTTGGTT AGGACTGCTC TAAAGATAAA AACAGAAGGT 
AACCTAGAGC AAGCAAATGA AGAACTTAGA GCAGTTATAA AGAAAATATG GAAGAAAACT 
AGCATGAAAT TACTTGACCA AGTTGTCCCT CCGGCTGGCG ATGATGAGGT AACCGTGGGG 
AAGTTCTATG CCACCTTCCT GATACAGGAC TACTTTAGGA AATTCAAGAA ACGCAAAGAA 
CAAGGACTGG TGGGGAAATA TCCTGCGAAG AATACCACGA TTGCTCTGCA GGCAGGACTA 
AGGACACTTC ATGATATTGG CCCTGAAATA CGCCGAGCTA TATCCTGTGA CTTGCAAGAT 
GATGAACCAG AGGAAAACAA TCCAGACGAG GAGGAGGAAG TTTATAAAAG GAATGGTGCC 
CTCTTTGGCA ACCATATAAA CCATATTAGC AGTGACAGAC GAGATTCATT TCAGCAGATC 
AACACCACGC ACCGTCCCTT ACATGTGCAG CGGCCTTCAA TTCCATCTGC AAGTGATACT 
GAGAAAAATA TATATCCTCA TACAGGAAAT TCTGTGTACC ACAATCATCA TAACCACAAC 
TCAGTAGGAA AACAAGTTCC AAACTCAACA AATGCCAATC TCAATAATGC CAACGTATCC 
AAAGTTGTTC ACGGAAAACA CGCTAATTTT GGAAGTCACG AGCATAGATC TGAAAATGGT 
TACCATTCGT ACTCTAGAGC TGACCATGAG AAGCGTAGAA GGCCAAGCAG TAGGAGAACC 
CGCTACTACG AAACATATAT TAGGTCTGAC TCTGGTGATG GCCGTCGACC AACTATTTGC 
CGTGAAGAAC GTGACATTCG AGACTATTGC AATGATGATC ATTATTTGGG AGAGCAGGAA 
TATTATAGTG GAGAAGAATA CTATGAGGAA GACAGCATGT TGTCAGGAAA CAGACATGTG 
TATGATTACC ACTGTAGACA TCACTGCCAT GACTCAGATT TTGAGAGACC AAAAGGTTAC 
CATCACCCAC ATGGGTTTTT TGAGGAAGAT GATTCACAGA CCTGTTATGA TACGAAAAGA 
TCTCCTAGGA GACGGCTGCT ACCTCCAACA CCAGCATCAA ATAGACGATC TTCCTTTAAT 
TTTGAATGCC TCCGCCGACA GAGTAGTCAA GATGATATAC CACTCTCTCC AAATTTCCAT 
CATCGCACTG CTTTACCACT TCACCTAATG CAACAGCAGG TCATGGCTGT TGCAGGTCTG 
GATTCCAGTA AAGCTCATAA ACATTCACCA AGTCGTTCCA CGCGTTCATG GGCTACCCCA 
CCTGCAACCC CTCCCAACAG GGACCATACC CCATACTATA CACCTCTAAT CCAAGTTGAC 
AGGGCTGAAT CTACAGAACA TATGAATGGT AGCCTGCCTT CCTTGCACAG GAGCTCCTGG 
TACACAGACG ACCCTGATAT CTCCTACCGA ACATTTACAC CAGCCAATTT AACAGTACCT 
AATGACTTTA GGCATAAACA CAGTGACAAA CAGAGGAGTG CAGACAGTTT GGTAGAAGCG 
GTACTTATAT CTGAAGGCCT AGGAAGGTAT GCAAAGGACC CTAAATTTGT TTCAGCTACA 
AAACATGAGA TTGCTGATGC TTGTGACATG ACTATCGATG AGATGGAAAG TGCAGCAAGT 
AATCTTCTCA ATGGAAATAT TAGCAATGGG ACCAATGGGG ATATGTTTCC CATTTTAAGC 
AGACAAGATT ATGAACTTCA AGATTTTGGT CCTGGCTACA GTGATGAAGA ACCAGAACCA 
GGACGATATG AAGAAGACTT AGCTGATGAA ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148470.1
CDS	335..6928
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292984.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292984.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATAACTCCG AAGACAGCGC AAGAATCTCC ATCACTTTTT TCCGTCTTTT CCGAGTGATG 
AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA GGAATCAGAA CTTTATTGTG GACATTTATT 
AAGTCATTTC AGGCTCTGCC TTATGTTGCC CTTCTGATAG CTATGCTGTT CTTCATCTAT 
GCTGTCATTG GAATGCAGGT ATTTGGAAAA GTGGCCATGA GAGACAACAA TCAAATAAAC 
AGAAACAACA ATTTTCAAAC TTTCCCCCAA GCTGTGCTGC TTCTCTTCAG GTGTGCAACT 
GGAGAAGCCT GGCAGGAAAT CATGCTGGCA TGTTTGCCTG GAAAACGCTG TGATCCAGAA 
TCTGATTATA ATCCAGGGGA AGAATACACA TGTGGAAGCA ATTTTGCCAT CATTTATTTT 
ATCAGTTTTT ATATGCTTTG TGCATTTTTG ATAATAAACT TATTTGTGGC TGTCATTATG 
GATAACTTTG ACTATTTGAC ACGTGACTGG TCCATTCTGG GTCCTCATCA TTTGGATGAG 
TTCAAAAGGA TATGGTCAGA ATATGACCCT GAAGCAAAGG GAAGGATAAA GCATCTTGAT 
GTGGTTACGT TACTGAGACG CATTCAGCCA CCACTTGGTT TTGGCAAATT ATGCCCTCAC 
CGGGTGGCCT GCAAGAGGTT AGTGGCCATG AATATGCCAC TAAATAGTGA CGGAACCGTC 
ATGTTCAATG CTACTTTGTT TGCTTTGGTT AGGACTGCTC TAAAGATAAA AACAGAAGGT 
AACCTAGAGC AAGCAAATGA AGAACTTAGA GCAGTTATAA AGAAAATATG GAAGAAAACT 
AGCATGAAAT TACTTGACCA AGTTGTCCCT CCGGCTGGCG ATGATGAGGT AACCGTGGGG 
AAGTTCTATG CCACCTTCCT GATACAGGAC TACTTTAGGA AATTCAAGAA ACGCAAAGAA 
CAAGGACTGG TGGGGAAATA TCCTGCGAAG AATACCACGA TTGCTCTGCA GGCAGGACTA 
AGGACACTTC ATGATATTGG CCCTGAAATA CGCCGAGCTA TATCCTGTGA CTTGCAAGAT 
GATGAACCAG AGGAAAACAA TCCAGACGAG GAGGAGGAAG TTTATAAAAG GAATGGTGCC 
CTCTTTGGCA ACCATATAAA CCATATTAGC AGTGACAGAC GAGATTCATT TCAGCAGATC 
AACACCACGC ACCGTCCCTT ACATGTGCAG CGGCCTTCAA TTCCATCTGC AAGTGATACT 
GAGAAAAATA TATATCCTCA TACAGGAAAT TCTGTGTACC ACAATCATCA TAACCACAAC 
TCAGTAGGAA AACAAGTTCC AAACTCAACA AATGCCAATC TCAATAATGC CAACGTATCC 
AAAGTTGTTC ACGGAAAACA CGCTAATTTT GGAAGTCACG AGCATAGATC TGAAAATGGT 
TACCATTCGT ACTCTAGAGC TGACCATGAG AAGCGTAGAA GGCCAAGCAG TAGGAGAACC 
CGCTACTACG AAACATATAT TAGGTCTGAC TCTGGTGATG GCCGTCGACC AACTATTTGC 
CGTGAAGAAC GTGACATTCG AGACTATTGC AATGATGATC ATTATTTGGG AGAGCAGGAA 
TATTATAGTG GAGAAGAATA CTATGAGGAA GACAGCATGT TGTCAGGAAA CAGACATGTG 
TATGATTACC ACTGTAGACA TCACTGCCAT GACTCAGATT TTGAGAGACC AAAAGGTTAC 
CATCACCCAC ATGGGTTTTT TGAGGAAGAT GATTCACAGA CCTGTTATGA TACGAAAAGA 
TCTCCTAGGA GACGGCTGCT ACCTCCAACA CCAGCATCAA ATAGACGATC TTCCTTTAAT 
TTTGAATGCC TCCGCCGACA GAGTAGTCAA GATGATATAC CACTCTCTCC AAATTTCCAT 
CATCGCACTG CTTTACCACT TCACCTAATG CAACAGCAGG TCATGGCTGT TGCAGGTCTG 
GATTCCAGTA AAGCTCATAA ACATTCACCA AGTCGTTCCA CGCGTTCATG GGCTACCCCA 
CCTGCAACCC CTCCCAACAG GGACCATACC CCATACTATA CACCTCTAAT CCAAGTTGAC 
AGGGCTGAAT CTACAGAACA TATGAATGGT AGCCTGCCTT CCTTGCACAG GAGCTCCTGG 
TACACAGACG ACCCTGATAT CTCCTACCGA ACATTTACAC CAGCCAATTT AACAGTACCT 
AATGACTTTA GGCATAAACA CAGTGACAAA CAGAGGAGTG CAGACAGTTT GGTAGAAGCG 
GTACTTATAT CTGAAGGCCT AGGAAGGTAT GCAAAGGACC CTAAATTTGT TTCAGCTACA 
AAACATGAGA TTGCTGATGC TTGTGACATG ACTATCGATG AGATGGAAAG TGCAGCAAGT 
AATCTTCTCA ATGGAAATAT TAGCAATGGG ACCAATGGGG ATATGTTTCC CATTTTAAGC 
AGACAAGATT ATGAACTTCA AGATTTTGGT CCTGGCTACA GTGATGAAGA ACCAGAACCA 
GGACGATATG AAGAAGACTT AGCTGATGAA ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292984.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148470.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.

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CloneID	OGa41342
Clone ID Related Accession (Same CDS sequence)	XM_015292984.1 , XM_015292984.2
Accession Version	XM_015292984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATAACTCCG AAGACAGCGC AAGAATCTCC ATCACTTTTT TCCGTCTTTT CCGAGTGATG 
AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA GGAATCAGAA CTTTATTGTG GACATTTATT 
AAGTCATTTC AGGCTCTGCC TTATGTTGCC CTTCTGATAG CTATGCTGTT CTTCATCTAT 
GCTGTCATTG GAATGCAGGT ATTTGGAAAA GTGGCCATGA GAGACAACAA TCAAATAAAC 
AGAAACAACA ATTTTCAAAC TTTCCCCCAA GCTGTGCTGC TTCTCTTCAG GTGTGCAACT 
GGAGAAGCCT GGCAGGAAAT CATGCTGGCA TGTTTGCCTG GAAAACGCTG TGATCCAGAA 
TCTGATTATA ATCCAGGGGA AGAATACACA TGTGGAAGCA ATTTTGCCAT CATTTATTTT 
ATCAGTTTTT ATATGCTTTG TGCATTTTTG ATAATAAACT TATTTGTGGC TGTCATTATG 
GATAACTTTG ACTATTTGAC ACGTGACTGG TCCATTCTGG GTCCTCATCA TTTGGATGAG 
TTCAAAAGGA TATGGTCAGA ATATGACCCT GAAGCAAAGG GAAGGATAAA GCATCTTGAT 
GTGGTTACGT TACTGAGACG CATTCAGCCA CCACTTGGTT TTGGCAAATT ATGCCCTCAC 
CGGGTGGCCT GCAAGAGGTT AGTGGCCATG AATATGCCAC TAAATAGTGA CGGAACCGTC 
ATGTTCAATG CTACTTTGTT TGCTTTGGTT AGGACTGCTC TAAAGATAAA AACAGAAGGT 
AACCTAGAGC AAGCAAATGA AGAACTTAGA GCAGTTATAA AGAAAATATG GAAGAAAACT 
AGCATGAAAT TACTTGACCA AGTTGTCCCT CCGGCTGGCG ATGATGAGGT AACCGTGGGG 
AAGTTCTATG CCACCTTCCT GATACAGGAC TACTTTAGGA AATTCAAGAA ACGCAAAGAA 
CAAGGACTGG TGGGGAAATA TCCTGCGAAG AATACCACGA TTGCTCTGCA GGCAGGACTA 
AGGACACTTC ATGATATTGG CCCTGAAATA CGCCGAGCTA TATCCTGTGA CTTGCAAGAT 
GATGAACCAG AGGAAAACAA TCCAGACGAG GAGGAGGAAG TTTATAAAAG GAATGGTGCC 
CTCTTTGGCA ACCATATAAA CCATATTAGC AGTGACAGAC GAGATTCATT TCAGCAGATC 
AACACCACGC ACCGTCCCTT ACATGTGCAG CGGCCTTCAA TTCCATCTGC AAGTGATACT 
GAGAAAAATA TATATCCTCA TACAGGAAAT TCTGTGTACC ACAATCATCA TAACCACAAC 
TCAGTAGGAA AACAAGTTCC AAACTCAACA AATGCCAATC TCAATAATGC CAACGTATCC 
AAAGTTGTTC ACGGAAAACA CGCTAATTTT GGAAGTCACG AGCATAGATC TGAAAATGGT 
TACCATTCGT ACTCTAGAGC TGACCATGAG AAGCGTAGAA GGCCAAGCAG TAGGAGAACC 
CGCTACTACG AAACATATAT TAGGTCTGAC TCTGGTGATG GCCGTCGACC AACTATTTGC 
CGTGAAGAAC GTGACATTCG AGACTATTGC AATGATGATC ATTATTTGGG AGAGCAGGAA 
TATTATAGTG GAGAAGAATA CTATGAGGAA GACAGCATGT TGTCAGGAAA CAGACATGTG 
TATGATTACC ACTGTAGACA TCACTGCCAT GACTCAGATT TTGAGAGACC AAAAGGTTAC 
CATCACCCAC ATGGGTTTTT TGAGGAAGAT GATTCACAGA CCTGTTATGA TACGAAAAGA 
TCTCCTAGGA GACGGCTGCT ACCTCCAACA CCAGCATCAA ATAGACGATC TTCCTTTAAT 
TTTGAATGCC TCCGCCGACA GAGTAGTCAA GATGATATAC CACTCTCTCC AAATTTCCAT 
CATCGCACTG CTTTACCACT TCACCTAATG CAACAGCAGG TCATGGCTGT TGCAGGTCTG 
GATTCCAGTA AAGCTCATAA ACATTCACCA AGTCGTTCCA CGCGTTCATG GGCTACCCCA 
CCTGCAACCC CTCCCAACAG GGACCATACC CCATACTATA CACCTCTAAT CCAAGTTGAC 
AGGGCTGAAT CTACAGAACA TATGAATGGT AGCCTGCCTT CCTTGCACAG GAGCTCCTGG 
TACACAGACG ACCCTGATAT CTCCTACCGA ACATTTACAC CAGCCAATTT AACAGTACCT 
AATGACTTTA GGCATAAACA CAGTGACAAA CAGAGGAGTG CAGACAGTTT GGTAGAAGCG 
GTACTTATAT CTGAAGGCCT AGGAAGGTAT GCAAAGGACC CTAAATTTGT TTCAGCTACA 
AAACATGAGA TTGCTGATGC TTGTGACATG ACTATCGATG AGATGGAAAG TGCAGCAAGT 
AATCTTCTCA ATGGAAATAT TAGCAATGGG ACCAATGGGG ATATGTTTCC CATTTTAAGC 
AGACAAGATT ATGAACTTCA AGATTTTGGT CCTGGCTACA GTGATGAAGA ACCAGAACCA 
GGACGATATG AAGAAGACTT AGCTGATGAA ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148470.1
CDS	335..6928
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292984.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292984.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATAACTCCG AAGACAGCGC AAGAATCTCC ATCACTTTTT TCCGTCTTTT CCGAGTGATG 
AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA GGAATCAGAA CTTTATTGTG GACATTTATT 
AAGTCATTTC AGGCTCTGCC TTATGTTGCC CTTCTGATAG CTATGCTGTT CTTCATCTAT 
GCTGTCATTG GAATGCAGGT ATTTGGAAAA GTGGCCATGA GAGACAACAA TCAAATAAAC 
AGAAACAACA ATTTTCAAAC TTTCCCCCAA GCTGTGCTGC TTCTCTTCAG GTGTGCAACT 
GGAGAAGCCT GGCAGGAAAT CATGCTGGCA TGTTTGCCTG GAAAACGCTG TGATCCAGAA 
TCTGATTATA ATCCAGGGGA AGAATACACA TGTGGAAGCA ATTTTGCCAT CATTTATTTT 
ATCAGTTTTT ATATGCTTTG TGCATTTTTG ATAATAAACT TATTTGTGGC TGTCATTATG 
GATAACTTTG ACTATTTGAC ACGTGACTGG TCCATTCTGG GTCCTCATCA TTTGGATGAG 
TTCAAAAGGA TATGGTCAGA ATATGACCCT GAAGCAAAGG GAAGGATAAA GCATCTTGAT 
GTGGTTACGT TACTGAGACG CATTCAGCCA CCACTTGGTT TTGGCAAATT ATGCCCTCAC 
CGGGTGGCCT GCAAGAGGTT AGTGGCCATG AATATGCCAC TAAATAGTGA CGGAACCGTC 
ATGTTCAATG CTACTTTGTT TGCTTTGGTT AGGACTGCTC TAAAGATAAA AACAGAAGGT 
AACCTAGAGC AAGCAAATGA AGAACTTAGA GCAGTTATAA AGAAAATATG GAAGAAAACT 
AGCATGAAAT TACTTGACCA AGTTGTCCCT CCGGCTGGCG ATGATGAGGT AACCGTGGGG 
AAGTTCTATG CCACCTTCCT GATACAGGAC TACTTTAGGA AATTCAAGAA ACGCAAAGAA 
CAAGGACTGG TGGGGAAATA TCCTGCGAAG AATACCACGA TTGCTCTGCA GGCAGGACTA 
AGGACACTTC ATGATATTGG CCCTGAAATA CGCCGAGCTA TATCCTGTGA CTTGCAAGAT 
GATGAACCAG AGGAAAACAA TCCAGACGAG GAGGAGGAAG TTTATAAAAG GAATGGTGCC 
CTCTTTGGCA ACCATATAAA CCATATTAGC AGTGACAGAC GAGATTCATT TCAGCAGATC 
AACACCACGC ACCGTCCCTT ACATGTGCAG CGGCCTTCAA TTCCATCTGC AAGTGATACT 
GAGAAAAATA TATATCCTCA TACAGGAAAT TCTGTGTACC ACAATCATCA TAACCACAAC 
TCAGTAGGAA AACAAGTTCC AAACTCAACA AATGCCAATC TCAATAATGC CAACGTATCC 
AAAGTTGTTC ACGGAAAACA CGCTAATTTT GGAAGTCACG AGCATAGATC TGAAAATGGT 
TACCATTCGT ACTCTAGAGC TGACCATGAG AAGCGTAGAA GGCCAAGCAG TAGGAGAACC 
CGCTACTACG AAACATATAT TAGGTCTGAC TCTGGTGATG GCCGTCGACC AACTATTTGC 
CGTGAAGAAC GTGACATTCG AGACTATTGC AATGATGATC ATTATTTGGG AGAGCAGGAA 
TATTATAGTG GAGAAGAATA CTATGAGGAA GACAGCATGT TGTCAGGAAA CAGACATGTG 
TATGATTACC ACTGTAGACA TCACTGCCAT GACTCAGATT TTGAGAGACC AAAAGGTTAC 
CATCACCCAC ATGGGTTTTT TGAGGAAGAT GATTCACAGA CCTGTTATGA TACGAAAAGA 
TCTCCTAGGA GACGGCTGCT ACCTCCAACA CCAGCATCAA ATAGACGATC TTCCTTTAAT 
TTTGAATGCC TCCGCCGACA GAGTAGTCAA GATGATATAC CACTCTCTCC AAATTTCCAT 
CATCGCACTG CTTTACCACT TCACCTAATG CAACAGCAGG TCATGGCTGT TGCAGGTCTG 
GATTCCAGTA AAGCTCATAA ACATTCACCA AGTCGTTCCA CGCGTTCATG GGCTACCCCA 
CCTGCAACCC CTCCCAACAG GGACCATACC CCATACTATA CACCTCTAAT CCAAGTTGAC 
AGGGCTGAAT CTACAGAACA TATGAATGGT AGCCTGCCTT CCTTGCACAG GAGCTCCTGG 
TACACAGACG ACCCTGATAT CTCCTACCGA ACATTTACAC CAGCCAATTT AACAGTACCT 
AATGACTTTA GGCATAAACA CAGTGACAAA CAGAGGAGTG CAGACAGTTT GGTAGAAGCG 
GTACTTATAT CTGAAGGCCT AGGAAGGTAT GCAAAGGACC CTAAATTTGT TTCAGCTACA 
AAACATGAGA TTGCTGATGC TTGTGACATG ACTATCGATG AGATGGAAAG TGCAGCAAGT 
AATCTTCTCA ATGGAAATAT TAGCAATGGG ACCAATGGGG ATATGTTTCC CATTTTAAGC 
AGACAAGATT ATGAACTTCA AGATTTTGGT CCTGGCTACA GTGATGAAGA ACCAGAACCA 
GGACGATATG AAGAAGACTT AGCTGATGAA ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292985.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148471.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X11, mRNA.

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CloneID	OGa41343
Clone ID Related Accession (Same CDS sequence)	XM_015292985.1 , XM_015292985.2
Accession Version	XM_015292985.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148471.1
CDS	335..6922
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292985.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292985.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292985.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148471.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.

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CloneID	OGa41343
Clone ID Related Accession (Same CDS sequence)	XM_015292985.1 , XM_015292985.2
Accession Version	XM_015292985.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292985.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148471.1
CDS	335..6922
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292985.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292985.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATCACTATT TCACTGATGC ATGGAACACT 
TTTGATGCCT TAATTGTTGT TGGTAGCGTC GTTGATATTG CTATAACAGA AGTTAATAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292986.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148472.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X16, mRNA.

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CloneID	OGa41344
Clone ID Related Accession (Same CDS sequence)	XM_015292986.2 , XM_015292986.1
Accession Version	XM_015292986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292986.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148472.1
CDS	335..6898
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292986.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292986.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292986.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148472.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X12, mRNA.

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CloneID	OGa41344
Clone ID Related Accession (Same CDS sequence)	XM_015292986.2 , XM_015292986.1
Accession Version	XM_015292986.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148472.1
CDS	335..6898
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292986.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292986.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292987.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148473.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.

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CloneID	OGa41345
Clone ID Related Accession (Same CDS sequence)	XM_015292987.1 , XM_015292987.2
Accession Version	XM_015292987.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148473.1
CDS	335..6883
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292987.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292987.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292987.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148473.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X18, mRNA.

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CloneID	OGa41345
Clone ID Related Accession (Same CDS sequence)	XM_015292987.1 , XM_015292987.2
Accession Version	XM_015292987.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292987.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148473.1
CDS	335..6883
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292987.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292987.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292988.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148474.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.

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CloneID	OGa41346
Clone ID Related Accession (Same CDS sequence)	XM_015292988.1 , XM_015292988.2
Accession Version	XM_015292988.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148474.1
CDS	335..6877
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292988.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292988.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292988.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148474.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X19, mRNA.

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CloneID	OGa41346
Clone ID Related Accession (Same CDS sequence)	XM_015292988.1 , XM_015292988.2
Accession Version	XM_015292988.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148474.1
CDS	335..6877
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292988.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292988.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292989.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148475.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X20, mRNA.

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CloneID	OGa41347
Clone ID Related Accession (Same CDS sequence)	XM_015292989.2 , XM_015292989.1
Accession Version	XM_015292989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148475.1
CDS	335..6877
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292989.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292989.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292989.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148475.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.

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CloneID	OGa41347
Clone ID Related Accession (Same CDS sequence)	XM_015292989.2 , XM_015292989.1
Accession Version	XM_015292989.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148475.1
CDS	335..6877
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292989.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292989.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATCCAAAGC CAACTGAAAC AGTCACAACT 
GATGAGTCTG GGAACTCCGA AGACAGCGCA AGAATCTCCA TCACTTTTTT CCGTCTTTTC 
CGAGTGATGA GGTTGGTGAA GCTGCTTAGC AGAGGAGAAG GAATCAGAAC TTTATTGTGG 
ACATTTATTA AGTCATTTCA GGCTCTGCCT TATGTTGCCC TTCTGATAGC TATGCTGTTC 
TTCATCTATG CTGTCATTGG AATGCAGGTA TTTGGAAAAG TGGCCATGAG AGACAACAAT 
CAAATAAACA GAAACAACAA TTTTCAAACT TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG 
TGTGCAACTG GAGAAGCCTG GCAGGAAATC ATGCTGGCAT GTTTGCCTGG AAAACGCTGT 
GATCCAGAAT CTGATTATAA TCCAGGGGAA GAATACACAT GTGGAAGCAA TTTTGCCATC 
ATTTATTTTA TCAGTTTTTA TATGCTTTGT GCATTTTTGA TAATAAACTT ATTTGTGGCT 
GTCATTATGG ATAACTTTGA CTATTTGACA CGTGACTGGT CCATTCTGGG TCCTCATCAT 
TTGGATGAGT TCAAAAGGAT ATGGTCAGAA TATGACCCTG AAGCAAAGGG AAGGATAAAG 
CATCTTGATG TGGTTACGTT ACTGAGACGC ATTCAGCCAC CACTTGGTTT TGGCAAATTA 
TGCCCTCACC GGGTGGCCTG CAAGAGGTTA GTGGCCATGA ATATGCCACT AAATAGTGAC 
GGAACCGTCA TGTTCAATGC TACTTTGTTT GCTTTGGTTA GGACTGCTCT AAAGATAAAA 
ACAGAAGGTA ACCTAGAGCA AGCAAATGAA GAACTTAGAG CAGTTATAAA GAAAATATGG 
AAGAAAACTA GCATGAAATT ACTTGACCAA GTTGTCCCTC CGGCTGGCGA TGATGAGGTA 
ACCGTGGGGA AGTTCTATGC CACCTTCCTG ATACAGGACT ACTTTAGGAA ATTCAAGAAA 
CGCAAAGAAC AAGGACTGGT GGGGAAATAT CCTGCGAAGA ATACCACGAT TGCTCTGCAG 
GCAGGACTAA GGACACTTCA TGATATTGGC CCTGAAATAC GCCGAGCTAT ATCCTGTGAC 
TTGCAAGATG ATGAACCAGA GGAAAACAAT CCAGACGAGG AGGAGGAAGT TTATAAAAGG 
AATGGTGCCC TCTTTGGCAA CCATATAAAC CATATTAGCA GTGACAGACG AGATTCATTT 
CAGCAGATCA ACACCACGCA CCGTCCCTTA CATGTGCAGC GGCCTTCAAT TCCATCTGCA 
AGTGATACTG AGAAAAATAT ATATCCTCAT ACAGGAAATT CTGTGTACCA CAATCATCAT 
AACCACAACT CAGTAGGAAA ACAAGTTCCA AACTCAACAA ATGCCAATCT CAATAATGCC 
AACGTATCCA AAGTTGTTCA CGGAAAACAC GCTAATTTTG GAAGTCACGA GCATAGATCT 
GAAAATGGTT ACCATTCGTA CTCTAGAGCT GACCATGAGA AGCGTAGAAG GCCAAGCAGT 
AGGAGAACCC GCTACTACGA AACATATATT AGGTCTGACT CTGGTGATGG CCGTCGACCA 
ACTATTTGCC GTGAAGAACG TGACATTCGA GACTATTGCA ATGATGATCA TTATTTGGGA 
GAGCAGGAAT ATTATAGTGG AGAAGAATAC TATGAGGAAG ACAGCATGTT GTCAGGAAAC 
AGACATGTGT ATGATTACCA CTGTAGACAT CACTGCCATG ACTCAGATTT TGAGAGACCA 
AAAGGTTACC ATCACCCACA TGGGTTTTTT GAGGAAGATG ATTCACAGAC CTGTTATGAT 
ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA CCTCCAACAC CAGCATCAAA TAGACGATCT 
TCCTTTAATT TTGAATGCCT CCGCCGACAG AGTAGTCAAG ATGATATACC ACTCTCTCCA 
AATTTCCATC ATCGCACTGC TTTACCACTT CACCTAATGC AACAGCAGGT CATGGCTGTT 
GCAGGTCTGG ATTCCAGTAA AGCTCATAAA CATTCACCAA GTCGTTCCAC GCGTTCATGG 
GCTACCCCAC CTGCAACCCC TCCCAACAGG GACCATACCC CATACTATAC ACCTCTAATC 
CAAGTTGACA GGGCTGAATC TACAGAACAT ATGAATGGTA GCCTGCCTTC CTTGCACAGG 
AGCTCCTGGT ACACAGACGA CCCTGATATC TCCTACCGAA CATTTACACC AGCCAATTTA 
ACAGTACCTA ATGACTTTAG GCATAAACAC AGTGACAAAC AGAGGAGTGC AGACAGTTTG 
GTAGAAGCGG TACTTATATC TGAAGGCCTA GGAAGGTATG CAAAGGACCC TAAATTTGTT 
TCAGCTACAA AACATGAGAT TGCTGATGCT TGTGACATGA CTATCGATGA GATGGAAAGT 
GCAGCAAGTA ATCTTCTCAA TGGAAATATT AGCAATGGGA CCAATGGGGA TATGTTTCCC 
ATTTTAAGCA GACAAGATTA TGAACTTCAA GATTTTGGTC CTGGCTACAG TGATGAAGAA 
CCAGAACCAG GACGATATGA AGAAGACTTA GCTGATGAAA TGATATGCAT TACAAGCTTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292990.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148476.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X21, mRNA.

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CloneID	OGa41348
Clone ID Related Accession (Same CDS sequence)	XM_015292990.2 , XM_015292990.1
Accession Version	XM_015292990.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292990.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACCA CCACAGCCCA GCCTGTAGGA TCTCTGTCCC AAAGAAAACG CCAGCAATAT 
GCCAAGAGCA AAAAACAGGG TAATACGTCT AATAGTCGGC CACCCCGTGC CCTTTTCTGT 
TTATCCCTCA ACAACCCAAT ACGAAGAGCC TGCATTAGTC TAGTAGAATG GAAACCATTT 
GACATATTTA TACTACTGTC TATTTTTGCC AATTGTGTGG CCTTAGCTGT TTACATCCCA 
TTCCCAGAAG ATGATTCTAA TTCAACTAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATAATTT TTACAGTTGA AACATTTTTG AAGATTATAG CATATGGATT ATTATTACAC 
CCCAATGCGT ATGTTAGAAA TGGATGGAAT TTATTGGATT TTGTAATAGT AGTAGTAGGA 
TTATTTAGTG TAATATTGGA ACAATTAACC AAAGAAACAG AAGGTGGCAG CCACTCAGGT 
GGCAAACCTG GAGGCTTTGA TGTCAAAGCC CTAAGAGCCT TTCGTGTATT GCGACCTCTC 
CGGCTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTCCTGA ACTCCATTAT AAAAGCCATG 
GTTCCCCTGC TCCATATTGC CCTTTTGGTA TTGTTTGTAA TCATAATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAATCTTGTT TTCTTATTGA TTCAGATATA 
CTAGTTGAAG AAGATCCAGC ACCTTGTGCA TTCTCAGGGA ATGGACGTCA GTGTGTCATG 
AATGGCACTG AATGTAAGGG TGGTTGGGTT GGACCGAATG GAGGCATAAC GAACTTCGAT 
AATTTTGCAT TTGCAATGCT GACTGTATTC CAGTGTATAA CCATGGAAGG ATGGACTGAT 
GTGTTATACT GGGTAAATGA TGCAATAGGA TGTGAATGGC CATGGATCTA TTTTGTTAGT 
CTTATCATCC TTGGCTCATT TTTCGTACTT AACCTGGTTC TTGGTGTACT TAGTGGGGAA 
TTTTCCAAGG AAAGAGAAAA AGCCAAGGCC CGAGGGGACT TTCAGAAGCT ACGTGAAAAA 
CAACAGCTTG AAGAAGATCT GAAGGGATAT TTAGACTGGA TTACACAGGC AGAAGACATT 
GATCCTGAGA ATGATGAAGA GGCTGATGAA GAAGGGAAAC GGAACAGGGT TACACTGGCT 
GACTTAATGG AAGAAAAGAA GAAAAGCAGA CTTAGTTGCT TTGGCCGGTC TTCCAATAAA 
CATGCAAGCA TGCCCACCAG CGAAACCGAG TCAGTGAACA CAGAGAATGT GAGTGGAGAA 
GGAGAGAACC CAGCATGCTG TGGGAGCTTA TGTCAAACCA TCTCAAAATC CAAGTTCAGT 
CGACGCTGGC GTCGCTGGAA TCGATTTAAT CGAAGAAAAT GTAGAGCTGC AGTAAAATCT 
GTCACGTTTT ATTGGCTTGT TATTGTCTTG GTCTTTCTGA ACACATTAAC TATCTCATCG 
GAACATTATA ACCAACCTGA CTGGCTGACC CAGATCCAAG ATATCGCAAA TAAAGTTCTT 
CTGGCTTTGT TCACCTGTGA AATGTTAGTA AAGATGTACA GCTTGGGTCT ACAGGCTTAT 
TTTGTTTCTC TTTTTAACCG CTTTGATTGC TTCGTTGTTT GTGGTGGCAT TGTTGAAACC 
ATTTTGGTGG AGTTGGAAAT TATGTCACCC CTTGGAATTT CAGTGTTTCG CTGTGTTCGT 
CTCCTGCGTA TTTTTAAAGT AACAAGGCAC TGGGCATCCC TGAGCAACTT GGTAGCATCC 
TTGTTAAACT CAATGAAGTC CATTGCTTCA CTGTTGCTTC TGCTTTTCCT CTTCATCATA 
ATCTTCTCGT TGCTTGGAAT GCAGCTGTTT GGAGGCAAAT TTAATTTTGA TGAAACTCAA 
ACAAAACGGA GCACCTTCGA TAATTTTCCA CAAGCTCTTT TAACTGTATT CCAGATTCTA 
ACAGGTGAAG ACTGGAATGC TGTTATGTAT GATGGCATAA TGGCGTATGG TGGCCCATCA 
TCGTCAGGCA TGATAGTCTG CATATATTTC ATTATCCTCT TCATCTGCGG TAACTATATC 
TTGCTAAATG TCTTCTTGGC CATTGCTGTG GATAACTTGG CAGATGCTGA AAGTCTAAAT 
ACAGCTCAGA AAGAAGAAGC AGAAGAAAAG GAAAGGAAAA AGAATGCAAG AAAAGAAAGT 
CTGGAAAATA AAAAAAGTGA GAAGTCAGAA GGTGACCAAA AGAAGCCCAA GGATAGTAAG 
GTGACAATTG CTGAATATGG AGAAGGAGAG GATGAAGATA AAGATCCCTA TCCACCCTGT 
GATGTACCAG TAGGTGAAGA TGAAGAAGAT GAGGAGGATG AACCTGAGGT ACCAGCAGGC 
CCACGTCCCC GGAGAATATC AGAACTAAAC ATGAAGGAGA AGATAACACC GATCCCTGAA 
GGGAGTGCCT TCTTCATTTT CAGCAGCACC AATCCAATTC GAGTGGGATG CCACAGGCTC 
ATCAATCACC ACATCTTTAC CAATCTCATC CTTGTCTTCA TCATGCTGAG CAGTGTTTCC 
CTAGCAGCAG AAGATCCGAT TCGCAGCCAC TCTTTTCGAA ATAATATACT TGGTTACTTT 
GACTATGCTT TCACAGCCAT CTTTACTGTT GAAATCCTGT TAAAGATCCT AGGGTATGCA 
GATTATGTCT TCACTAGTAT GTTTACATTT GAGATCATTT TGAAGATGAC AGCTTTCGGA 
GCATTCCTTC ATAAAGGGTC CTTCTGCAGA AATTACTTTA ATTTGCTGGA TTTGCTGGTT 
GTGGGTGTTT CTCTGGTATC ATTTGGTATT CAGTCAAGTG CTATCTCAGT TGTGAAGATC 
CTCAGAGTTT TGAGAGTCTT GAGGCCCCTT AGGGCTATAA ACAGAGCAAA AGGACTTAAG 
CACGTTGTTC AGTGCGTCTT TGTGGCTATT AGAACTATTG GTAATATCAT GATTGTTACA 
ACTCTGCTGC AGTTCATGTT TGCTTGTATT GGAGTGCAGC TCTTCAAGGG AAAATTCTAC 
AAGTGCACTG ATGAGGCAAA ACAGAATCCA GAGGAATGCC GGGGGATATA CATTGTTTAT 
AAAGATGGAG ATGTTGATAA TCCAATGGTC AAAGAGAGGG TCTGGCAAAA TAGTGATTTC 
AACTTCGACA ATGTTCTGTC TGCTATGATG GCCCTTTTCA CAGTGTCTAC TTTTGAAGGC 
TGGCCAGCGC TGCTGTACAA AGCCATTGAT TCCAATGGCG AGAATGTAGG ACCTGTGTAC 
AACTACAGAG TGGAGATCTC CATTTTTTTC ATCATCTACA TCATCATTAT TGCTTTCTTT 
ATGATGAACA TATTTGTTGG TTTTGTCATT GTTACCTTTC AAGAACAGGG AGAACAAGAA 
TACAAGAACT GTGAGCTGGA CAAAAATCAG CGTCAGTGCG TGGAATATGC CTTGAAGGCT 
CGTCCTCTTC GTAGATACAT TCCAAAAAAT CCTTACCAGT ATAAGTTCTG GTATGTGGTG 
AATTCTACTG GATTTGAATA CATCATGTTT GTCCTCATCA TGCTGAACAC ACTTTGCCTG 
GCTATGCAGC ACTATGGACA GTCTAAGCTC TTTAATGATG CGATGGACAT TATGAATATG 
GTTTTCACGG GAGTGTTCAC TGTTGAAATG GTTTTGAAGC TGATTGCATT CAAACCCAAG 
ATTTTTGTAA GAAAAAAGGA AAGATGGCTA GGCTATTTTA GTGATGCCTG GAATACGTTT 
GACTCCCTCA TCGTTATTGG CAGCATAGTA GACGTCGTTC TCAGTGAAGC TGATCACTAT 
TTCACTGATG CATGGAACAC TTTTGATGCC TTAATTGTTG TTGGTAGCGT CGTTGATATT 
GCTATAACAG AAGTTAATCC AAAGCCAACT GAAACAGTCA CAACTGATGA GTCTGGGAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148476.1
CDS	260..6787
Translation	MNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292990.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292990.2

ORF Insert Sequence:

ATGAATACCA CCACAGCCCA GCCTGTAGGA TCTCTGTCCC AAAGAAAACG CCAGCAATAT 
GCCAAGAGCA AAAAACAGGG TAATACGTCT AATAGTCGGC CACCCCGTGC CCTTTTCTGT 
TTATCCCTCA ACAACCCAAT ACGAAGAGCC TGCATTAGTC TAGTAGAATG GAAACCATTT 
GACATATTTA TACTACTGTC TATTTTTGCC AATTGTGTGG CCTTAGCTGT TTACATCCCA 
TTCCCAGAAG ATGATTCTAA TTCAACTAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATAATTT TTACAGTTGA AACATTTTTG AAGATTATAG CATATGGATT ATTATTACAC 
CCCAATGCGT ATGTTAGAAA TGGATGGAAT TTATTGGATT TTGTAATAGT AGTAGTAGGA 
TTATTTAGTG TAATATTGGA ACAATTAACC AAAGAAACAG AAGGTGGCAG CCACTCAGGT 
GGCAAACCTG GAGGCTTTGA TGTCAAAGCC CTAAGAGCCT TTCGTGTATT GCGACCTCTC 
CGGCTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTCCTGA ACTCCATTAT AAAAGCCATG 
GTTCCCCTGC TCCATATTGC CCTTTTGGTA TTGTTTGTAA TCATAATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAATCTTGTT TTCTTATTGA TTCAGATATA 
CTAGTTGAAG AAGATCCAGC ACCTTGTGCA TTCTCAGGGA ATGGACGTCA GTGTGTCATG 
AATGGCACTG AATGTAAGGG TGGTTGGGTT GGACCGAATG GAGGCATAAC GAACTTCGAT 
AATTTTGCAT TTGCAATGCT GACTGTATTC CAGTGTATAA CCATGGAAGG ATGGACTGAT 
GTGTTATACT GGGTAAATGA TGCAATAGGA TGTGAATGGC CATGGATCTA TTTTGTTAGT 
CTTATCATCC TTGGCTCATT TTTCGTACTT AACCTGGTTC TTGGTGTACT TAGTGGGGAA 
TTTTCCAAGG AAAGAGAAAA AGCCAAGGCC CGAGGGGACT TTCAGAAGCT ACGTGAAAAA 
CAACAGCTTG AAGAAGATCT GAAGGGATAT TTAGACTGGA TTACACAGGC AGAAGACATT 
GATCCTGAGA ATGATGAAGA GGCTGATGAA GAAGGGAAAC GGAACAGGGT TACACTGGCT 
GACTTAATGG AAGAAAAGAA GAAAAGCAGA CTTAGTTGCT TTGGCCGGTC TTCCAATAAA 
CATGCAAGCA TGCCCACCAG CGAAACCGAG TCAGTGAACA CAGAGAATGT GAGTGGAGAA 
GGAGAGAACC CAGCATGCTG TGGGAGCTTA TGTCAAACCA TCTCAAAATC CAAGTTCAGT 
CGACGCTGGC GTCGCTGGAA TCGATTTAAT CGAAGAAAAT GTAGAGCTGC AGTAAAATCT 
GTCACGTTTT ATTGGCTTGT TATTGTCTTG GTCTTTCTGA ACACATTAAC TATCTCATCG 
GAACATTATA ACCAACCTGA CTGGCTGACC CAGATCCAAG ATATCGCAAA TAAAGTTCTT 
CTGGCTTTGT TCACCTGTGA AATGTTAGTA AAGATGTACA GCTTGGGTCT ACAGGCTTAT 
TTTGTTTCTC TTTTTAACCG CTTTGATTGC TTCGTTGTTT GTGGTGGCAT TGTTGAAACC 
ATTTTGGTGG AGTTGGAAAT TATGTCACCC CTTGGAATTT CAGTGTTTCG CTGTGTTCGT 
CTCCTGCGTA TTTTTAAAGT AACAAGGCAC TGGGCATCCC TGAGCAACTT GGTAGCATCC 
TTGTTAAACT CAATGAAGTC CATTGCTTCA CTGTTGCTTC TGCTTTTCCT CTTCATCATA 
ATCTTCTCGT TGCTTGGAAT GCAGCTGTTT GGAGGCAAAT TTAATTTTGA TGAAACTCAA 
ACAAAACGGA GCACCTTCGA TAATTTTCCA CAAGCTCTTT TAACTGTATT CCAGATTCTA 
ACAGGTGAAG ACTGGAATGC TGTTATGTAT GATGGCATAA TGGCGTATGG TGGCCCATCA 
TCGTCAGGCA TGATAGTCTG CATATATTTC ATTATCCTCT TCATCTGCGG TAACTATATC 
TTGCTAAATG TCTTCTTGGC CATTGCTGTG GATAACTTGG CAGATGCTGA AAGTCTAAAT 
ACAGCTCAGA AAGAAGAAGC AGAAGAAAAG GAAAGGAAAA AGAATGCAAG AAAAGAAAGT 
CTGGAAAATA AAAAAAGTGA GAAGTCAGAA GGTGACCAAA AGAAGCCCAA GGATAGTAAG 
GTGACAATTG CTGAATATGG AGAAGGAGAG GATGAAGATA AAGATCCCTA TCCACCCTGT 
GATGTACCAG TAGGTGAAGA TGAAGAAGAT GAGGAGGATG AACCTGAGGT ACCAGCAGGC 
CCACGTCCCC GGAGAATATC AGAACTAAAC ATGAAGGAGA AGATAACACC GATCCCTGAA 
GGGAGTGCCT TCTTCATTTT CAGCAGCACC AATCCAATTC GAGTGGGATG CCACAGGCTC 
ATCAATCACC ACATCTTTAC CAATCTCATC CTTGTCTTCA TCATGCTGAG CAGTGTTTCC 
CTAGCAGCAG AAGATCCGAT TCGCAGCCAC TCTTTTCGAA ATAATATACT TGGTTACTTT 
GACTATGCTT TCACAGCCAT CTTTACTGTT GAAATCCTGT TAAAGATCCT AGGGTATGCA 
GATTATGTCT TCACTAGTAT GTTTACATTT GAGATCATTT TGAAGATGAC AGCTTTCGGA 
GCATTCCTTC ATAAAGGGTC CTTCTGCAGA AATTACTTTA ATTTGCTGGA TTTGCTGGTT 
GTGGGTGTTT CTCTGGTATC ATTTGGTATT CAGTCAAGTG CTATCTCAGT TGTGAAGATC 
CTCAGAGTTT TGAGAGTCTT GAGGCCCCTT AGGGCTATAA ACAGAGCAAA AGGACTTAAG 
CACGTTGTTC AGTGCGTCTT TGTGGCTATT AGAACTATTG GTAATATCAT GATTGTTACA 
ACTCTGCTGC AGTTCATGTT TGCTTGTATT GGAGTGCAGC TCTTCAAGGG AAAATTCTAC 
AAGTGCACTG ATGAGGCAAA ACAGAATCCA GAGGAATGCC GGGGGATATA CATTGTTTAT 
AAAGATGGAG ATGTTGATAA TCCAATGGTC AAAGAGAGGG TCTGGCAAAA TAGTGATTTC 
AACTTCGACA ATGTTCTGTC TGCTATGATG GCCCTTTTCA CAGTGTCTAC TTTTGAAGGC 
TGGCCAGCGC TGCTGTACAA AGCCATTGAT TCCAATGGCG AGAATGTAGG ACCTGTGTAC 
AACTACAGAG TGGAGATCTC CATTTTTTTC ATCATCTACA TCATCATTAT TGCTTTCTTT 
ATGATGAACA TATTTGTTGG TTTTGTCATT GTTACCTTTC AAGAACAGGG AGAACAAGAA 
TACAAGAACT GTGAGCTGGA CAAAAATCAG CGTCAGTGCG TGGAATATGC CTTGAAGGCT 
CGTCCTCTTC GTAGATACAT TCCAAAAAAT CCTTACCAGT ATAAGTTCTG GTATGTGGTG 
AATTCTACTG GATTTGAATA CATCATGTTT GTCCTCATCA TGCTGAACAC ACTTTGCCTG 
GCTATGCAGC ACTATGGACA GTCTAAGCTC TTTAATGATG CGATGGACAT TATGAATATG 
GTTTTCACGG GAGTGTTCAC TGTTGAAATG GTTTTGAAGC TGATTGCATT CAAACCCAAG 
ATTTTTGTAA GAAAAAAGGA AAGATGGCTA GGCTATTTTA GTGATGCCTG GAATACGTTT 
GACTCCCTCA TCGTTATTGG CAGCATAGTA GACGTCGTTC TCAGTGAAGC TGATCACTAT 
TTCACTGATG CATGGAACAC TTTTGATGCC TTAATTGTTG TTGGTAGCGT CGTTGATATT 
GCTATAACAG AAGTTAATCC AAAGCCAACT GAAACAGTCA CAACTGATGA GTCTGGGAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292990.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148476.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.

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CloneID	OGa41348
Clone ID Related Accession (Same CDS sequence)	XM_015292990.2 , XM_015292990.1
Accession Version	XM_015292990.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACCA CCACAGCCCA GCCTGTAGGA TCTCTGTCCC AAAGAAAACG CCAGCAATAT 
GCCAAGAGCA AAAAACAGGG TAATACGTCT AATAGTCGGC CACCCCGTGC CCTTTTCTGT 
TTATCCCTCA ACAACCCAAT ACGAAGAGCC TGCATTAGTC TAGTAGAATG GAAACCATTT 
GACATATTTA TACTACTGTC TATTTTTGCC AATTGTGTGG CCTTAGCTGT TTACATCCCA 
TTCCCAGAAG ATGATTCTAA TTCAACTAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATAATTT TTACAGTTGA AACATTTTTG AAGATTATAG CATATGGATT ATTATTACAC 
CCCAATGCGT ATGTTAGAAA TGGATGGAAT TTATTGGATT TTGTAATAGT AGTAGTAGGA 
TTATTTAGTG TAATATTGGA ACAATTAACC AAAGAAACAG AAGGTGGCAG CCACTCAGGT 
GGCAAACCTG GAGGCTTTGA TGTCAAAGCC CTAAGAGCCT TTCGTGTATT GCGACCTCTC 
CGGCTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTCCTGA ACTCCATTAT AAAAGCCATG 
GTTCCCCTGC TCCATATTGC CCTTTTGGTA TTGTTTGTAA TCATAATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAATCTTGTT TTCTTATTGA TTCAGATATA 
CTAGTTGAAG AAGATCCAGC ACCTTGTGCA TTCTCAGGGA ATGGACGTCA GTGTGTCATG 
AATGGCACTG AATGTAAGGG TGGTTGGGTT GGACCGAATG GAGGCATAAC GAACTTCGAT 
AATTTTGCAT TTGCAATGCT GACTGTATTC CAGTGTATAA CCATGGAAGG ATGGACTGAT 
GTGTTATACT GGGTAAATGA TGCAATAGGA TGTGAATGGC CATGGATCTA TTTTGTTAGT 
CTTATCATCC TTGGCTCATT TTTCGTACTT AACCTGGTTC TTGGTGTACT TAGTGGGGAA 
TTTTCCAAGG AAAGAGAAAA AGCCAAGGCC CGAGGGGACT TTCAGAAGCT ACGTGAAAAA 
CAACAGCTTG AAGAAGATCT GAAGGGATAT TTAGACTGGA TTACACAGGC AGAAGACATT 
GATCCTGAGA ATGATGAAGA GGCTGATGAA GAAGGGAAAC GGAACAGGGT TACACTGGCT 
GACTTAATGG AAGAAAAGAA GAAAAGCAGA CTTAGTTGCT TTGGCCGGTC TTCCAATAAA 
CATGCAAGCA TGCCCACCAG CGAAACCGAG TCAGTGAACA CAGAGAATGT GAGTGGAGAA 
GGAGAGAACC CAGCATGCTG TGGGAGCTTA TGTCAAACCA TCTCAAAATC CAAGTTCAGT 
CGACGCTGGC GTCGCTGGAA TCGATTTAAT CGAAGAAAAT GTAGAGCTGC AGTAAAATCT 
GTCACGTTTT ATTGGCTTGT TATTGTCTTG GTCTTTCTGA ACACATTAAC TATCTCATCG 
GAACATTATA ACCAACCTGA CTGGCTGACC CAGATCCAAG ATATCGCAAA TAAAGTTCTT 
CTGGCTTTGT TCACCTGTGA AATGTTAGTA AAGATGTACA GCTTGGGTCT ACAGGCTTAT 
TTTGTTTCTC TTTTTAACCG CTTTGATTGC TTCGTTGTTT GTGGTGGCAT TGTTGAAACC 
ATTTTGGTGG AGTTGGAAAT TATGTCACCC CTTGGAATTT CAGTGTTTCG CTGTGTTCGT 
CTCCTGCGTA TTTTTAAAGT AACAAGGCAC TGGGCATCCC TGAGCAACTT GGTAGCATCC 
TTGTTAAACT CAATGAAGTC CATTGCTTCA CTGTTGCTTC TGCTTTTCCT CTTCATCATA 
ATCTTCTCGT TGCTTGGAAT GCAGCTGTTT GGAGGCAAAT TTAATTTTGA TGAAACTCAA 
ACAAAACGGA GCACCTTCGA TAATTTTCCA CAAGCTCTTT TAACTGTATT CCAGATTCTA 
ACAGGTGAAG ACTGGAATGC TGTTATGTAT GATGGCATAA TGGCGTATGG TGGCCCATCA 
TCGTCAGGCA TGATAGTCTG CATATATTTC ATTATCCTCT TCATCTGCGG TAACTATATC 
TTGCTAAATG TCTTCTTGGC CATTGCTGTG GATAACTTGG CAGATGCTGA AAGTCTAAAT 
ACAGCTCAGA AAGAAGAAGC AGAAGAAAAG GAAAGGAAAA AGAATGCAAG AAAAGAAAGT 
CTGGAAAATA AAAAAAGTGA GAAGTCAGAA GGTGACCAAA AGAAGCCCAA GGATAGTAAG 
GTGACAATTG CTGAATATGG AGAAGGAGAG GATGAAGATA AAGATCCCTA TCCACCCTGT 
GATGTACCAG TAGGTGAAGA TGAAGAAGAT GAGGAGGATG AACCTGAGGT ACCAGCAGGC 
CCACGTCCCC GGAGAATATC AGAACTAAAC ATGAAGGAGA AGATAACACC GATCCCTGAA 
GGGAGTGCCT TCTTCATTTT CAGCAGCACC AATCCAATTC GAGTGGGATG CCACAGGCTC 
ATCAATCACC ACATCTTTAC CAATCTCATC CTTGTCTTCA TCATGCTGAG CAGTGTTTCC 
CTAGCAGCAG AAGATCCGAT TCGCAGCCAC TCTTTTCGAA ATAATATACT TGGTTACTTT 
GACTATGCTT TCACAGCCAT CTTTACTGTT GAAATCCTGT TAAAGATCCT AGGGTATGCA 
GATTATGTCT TCACTAGTAT GTTTACATTT GAGATCATTT TGAAGATGAC AGCTTTCGGA 
GCATTCCTTC ATAAAGGGTC CTTCTGCAGA AATTACTTTA ATTTGCTGGA TTTGCTGGTT 
GTGGGTGTTT CTCTGGTATC ATTTGGTATT CAGTCAAGTG CTATCTCAGT TGTGAAGATC 
CTCAGAGTTT TGAGAGTCTT GAGGCCCCTT AGGGCTATAA ACAGAGCAAA AGGACTTAAG 
CACGTTGTTC AGTGCGTCTT TGTGGCTATT AGAACTATTG GTAATATCAT GATTGTTACA 
ACTCTGCTGC AGTTCATGTT TGCTTGTATT GGAGTGCAGC TCTTCAAGGG AAAATTCTAC 
AAGTGCACTG ATGAGGCAAA ACAGAATCCA GAGGAATGCC GGGGGATATA CATTGTTTAT 
AAAGATGGAG ATGTTGATAA TCCAATGGTC AAAGAGAGGG TCTGGCAAAA TAGTGATTTC 
AACTTCGACA ATGTTCTGTC TGCTATGATG GCCCTTTTCA CAGTGTCTAC TTTTGAAGGC 
TGGCCAGCGC TGCTGTACAA AGCCATTGAT TCCAATGGCG AGAATGTAGG ACCTGTGTAC 
AACTACAGAG TGGAGATCTC CATTTTTTTC ATCATCTACA TCATCATTAT TGCTTTCTTT 
ATGATGAACA TATTTGTTGG TTTTGTCATT GTTACCTTTC AAGAACAGGG AGAACAAGAA 
TACAAGAACT GTGAGCTGGA CAAAAATCAG CGTCAGTGCG TGGAATATGC CTTGAAGGCT 
CGTCCTCTTC GTAGATACAT TCCAAAAAAT CCTTACCAGT ATAAGTTCTG GTATGTGGTG 
AATTCTACTG GATTTGAATA CATCATGTTT GTCCTCATCA TGCTGAACAC ACTTTGCCTG 
GCTATGCAGC ACTATGGACA GTCTAAGCTC TTTAATGATG CGATGGACAT TATGAATATG 
GTTTTCACGG GAGTGTTCAC TGTTGAAATG GTTTTGAAGC TGATTGCATT CAAACCCAAG 
ATTTTTGTAA GAAAAAAGGA AAGATGGCTA GGCTATTTTA GTGATGCCTG GAATACGTTT 
GACTCCCTCA TCGTTATTGG CAGCATAGTA GACGTCGTTC TCAGTGAAGC TGATCACTAT 
TTCACTGATG CATGGAACAC TTTTGATGCC TTAATTGTTG TTGGTAGCGT CGTTGATATT 
GCTATAACAG AAGTTAATCC AAAGCCAACT GAAACAGTCA CAACTGATGA GTCTGGGAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148476.1
CDS	253..6780
Translation	MNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292990.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292990.1

ORF Insert Sequence:

ATGAATACCA CCACAGCCCA GCCTGTAGGA TCTCTGTCCC AAAGAAAACG CCAGCAATAT 
GCCAAGAGCA AAAAACAGGG TAATACGTCT AATAGTCGGC CACCCCGTGC CCTTTTCTGT 
TTATCCCTCA ACAACCCAAT ACGAAGAGCC TGCATTAGTC TAGTAGAATG GAAACCATTT 
GACATATTTA TACTACTGTC TATTTTTGCC AATTGTGTGG CCTTAGCTGT TTACATCCCA 
TTCCCAGAAG ATGATTCTAA TTCAACTAAT CATAACTTGG AAAAAGTAGA ATATGCCTTC 
CTGATAATTT TTACAGTTGA AACATTTTTG AAGATTATAG CATATGGATT ATTATTACAC 
CCCAATGCGT ATGTTAGAAA TGGATGGAAT TTATTGGATT TTGTAATAGT AGTAGTAGGA 
TTATTTAGTG TAATATTGGA ACAATTAACC AAAGAAACAG AAGGTGGCAG CCACTCAGGT 
GGCAAACCTG GAGGCTTTGA TGTCAAAGCC CTAAGAGCCT TTCGTGTATT GCGACCTCTC 
CGGCTTGTAT CAGGAGTGCC CAGTTTACAA GTTGTCCTGA ACTCCATTAT AAAAGCCATG 
GTTCCCCTGC TCCATATTGC CCTTTTGGTA TTGTTTGTAA TCATAATCTA TGCTATTATA 
GGATTGGAAC TTTTTATTGG AAAAATGCAC AAATCTTGTT TTCTTATTGA TTCAGATATA 
CTAGTTGAAG AAGATCCAGC ACCTTGTGCA TTCTCAGGGA ATGGACGTCA GTGTGTCATG 
AATGGCACTG AATGTAAGGG TGGTTGGGTT GGACCGAATG GAGGCATAAC GAACTTCGAT 
AATTTTGCAT TTGCAATGCT GACTGTATTC CAGTGTATAA CCATGGAAGG ATGGACTGAT 
GTGTTATACT GGGTAAATGA TGCAATAGGA TGTGAATGGC CATGGATCTA TTTTGTTAGT 
CTTATCATCC TTGGCTCATT TTTCGTACTT AACCTGGTTC TTGGTGTACT TAGTGGGGAA 
TTTTCCAAGG AAAGAGAAAA AGCCAAGGCC CGAGGGGACT TTCAGAAGCT ACGTGAAAAA 
CAACAGCTTG AAGAAGATCT GAAGGGATAT TTAGACTGGA TTACACAGGC AGAAGACATT 
GATCCTGAGA ATGATGAAGA GGCTGATGAA GAAGGGAAAC GGAACAGGGT TACACTGGCT 
GACTTAATGG AAGAAAAGAA GAAAAGCAGA CTTAGTTGCT TTGGCCGGTC TTCCAATAAA 
CATGCAAGCA TGCCCACCAG CGAAACCGAG TCAGTGAACA CAGAGAATGT GAGTGGAGAA 
GGAGAGAACC CAGCATGCTG TGGGAGCTTA TGTCAAACCA TCTCAAAATC CAAGTTCAGT 
CGACGCTGGC GTCGCTGGAA TCGATTTAAT CGAAGAAAAT GTAGAGCTGC AGTAAAATCT 
GTCACGTTTT ATTGGCTTGT TATTGTCTTG GTCTTTCTGA ACACATTAAC TATCTCATCG 
GAACATTATA ACCAACCTGA CTGGCTGACC CAGATCCAAG ATATCGCAAA TAAAGTTCTT 
CTGGCTTTGT TCACCTGTGA AATGTTAGTA AAGATGTACA GCTTGGGTCT ACAGGCTTAT 
TTTGTTTCTC TTTTTAACCG CTTTGATTGC TTCGTTGTTT GTGGTGGCAT TGTTGAAACC 
ATTTTGGTGG AGTTGGAAAT TATGTCACCC CTTGGAATTT CAGTGTTTCG CTGTGTTCGT 
CTCCTGCGTA TTTTTAAAGT AACAAGGCAC TGGGCATCCC TGAGCAACTT GGTAGCATCC 
TTGTTAAACT CAATGAAGTC CATTGCTTCA CTGTTGCTTC TGCTTTTCCT CTTCATCATA 
ATCTTCTCGT TGCTTGGAAT GCAGCTGTTT GGAGGCAAAT TTAATTTTGA TGAAACTCAA 
ACAAAACGGA GCACCTTCGA TAATTTTCCA CAAGCTCTTT TAACTGTATT CCAGATTCTA 
ACAGGTGAAG ACTGGAATGC TGTTATGTAT GATGGCATAA TGGCGTATGG TGGCCCATCA 
TCGTCAGGCA TGATAGTCTG CATATATTTC ATTATCCTCT TCATCTGCGG TAACTATATC 
TTGCTAAATG TCTTCTTGGC CATTGCTGTG GATAACTTGG CAGATGCTGA AAGTCTAAAT 
ACAGCTCAGA AAGAAGAAGC AGAAGAAAAG GAAAGGAAAA AGAATGCAAG AAAAGAAAGT 
CTGGAAAATA AAAAAAGTGA GAAGTCAGAA GGTGACCAAA AGAAGCCCAA GGATAGTAAG 
GTGACAATTG CTGAATATGG AGAAGGAGAG GATGAAGATA AAGATCCCTA TCCACCCTGT 
GATGTACCAG TAGGTGAAGA TGAAGAAGAT GAGGAGGATG AACCTGAGGT ACCAGCAGGC 
CCACGTCCCC GGAGAATATC AGAACTAAAC ATGAAGGAGA AGATAACACC GATCCCTGAA 
GGGAGTGCCT TCTTCATTTT CAGCAGCACC AATCCAATTC GAGTGGGATG CCACAGGCTC 
ATCAATCACC ACATCTTTAC CAATCTCATC CTTGTCTTCA TCATGCTGAG CAGTGTTTCC 
CTAGCAGCAG AAGATCCGAT TCGCAGCCAC TCTTTTCGAA ATAATATACT TGGTTACTTT 
GACTATGCTT TCACAGCCAT CTTTACTGTT GAAATCCTGT TAAAGATCCT AGGGTATGCA 
GATTATGTCT TCACTAGTAT GTTTACATTT GAGATCATTT TGAAGATGAC AGCTTTCGGA 
GCATTCCTTC ATAAAGGGTC CTTCTGCAGA AATTACTTTA ATTTGCTGGA TTTGCTGGTT 
GTGGGTGTTT CTCTGGTATC ATTTGGTATT CAGTCAAGTG CTATCTCAGT TGTGAAGATC 
CTCAGAGTTT TGAGAGTCTT GAGGCCCCTT AGGGCTATAA ACAGAGCAAA AGGACTTAAG 
CACGTTGTTC AGTGCGTCTT TGTGGCTATT AGAACTATTG GTAATATCAT GATTGTTACA 
ACTCTGCTGC AGTTCATGTT TGCTTGTATT GGAGTGCAGC TCTTCAAGGG AAAATTCTAC 
AAGTGCACTG ATGAGGCAAA ACAGAATCCA GAGGAATGCC GGGGGATATA CATTGTTTAT 
AAAGATGGAG ATGTTGATAA TCCAATGGTC AAAGAGAGGG TCTGGCAAAA TAGTGATTTC 
AACTTCGACA ATGTTCTGTC TGCTATGATG GCCCTTTTCA CAGTGTCTAC TTTTGAAGGC 
TGGCCAGCGC TGCTGTACAA AGCCATTGAT TCCAATGGCG AGAATGTAGG ACCTGTGTAC 
AACTACAGAG TGGAGATCTC CATTTTTTTC ATCATCTACA TCATCATTAT TGCTTTCTTT 
ATGATGAACA TATTTGTTGG TTTTGTCATT GTTACCTTTC AAGAACAGGG AGAACAAGAA 
TACAAGAACT GTGAGCTGGA CAAAAATCAG CGTCAGTGCG TGGAATATGC CTTGAAGGCT 
CGTCCTCTTC GTAGATACAT TCCAAAAAAT CCTTACCAGT ATAAGTTCTG GTATGTGGTG 
AATTCTACTG GATTTGAATA CATCATGTTT GTCCTCATCA TGCTGAACAC ACTTTGCCTG 
GCTATGCAGC ACTATGGACA GTCTAAGCTC TTTAATGATG CGATGGACAT TATGAATATG 
GTTTTCACGG GAGTGTTCAC TGTTGAAATG GTTTTGAAGC TGATTGCATT CAAACCCAAG 
ATTTTTGTAA GAAAAAAGGA AAGATGGCTA GGCTATTTTA GTGATGCCTG GAATACGTTT 
GACTCCCTCA TCGTTATTGG CAGCATAGTA GACGTCGTTC TCAGTGAAGC TGATCACTAT 
TTCACTGATG CATGGAACAC TTTTGATGCC TTAATTGTTG TTGGTAGCGT CGTTGATATT 
GCTATAACAG AAGTTAATCC AAAGCCAACT GAAACAGTCA CAACTGATGA GTCTGGGAAC 
TCCGAAGACA GCGCAAGAAT CTCCATCACT TTTTTCCGTC TTTTCCGAGT GATGAGGTTG 
GTGAAGCTGC TTAGCAGAGG AGAAGGAATC AGAACTTTAT TGTGGACATT TATTAAGTCA 
TTTCAGGCTC TGCCTTATGT TGCCCTTCTG ATAGCTATGC TGTTCTTCAT CTATGCTGTC 
ATTGGAATGC AGGTATTTGG AAAAGTGGCC ATGAGAGACA ACAATCAAAT AAACAGAAAC 
AACAATTTTC AAACTTTCCC CCAAGCTGTG CTGCTTCTCT TCAGGTGTGC AACTGGAGAA 
GCCTGGCAGG AAATCATGCT GGCATGTTTG CCTGGAAAAC GCTGTGATCC AGAATCTGAT 
TATAATCCAG GGGAAGAATA CACATGTGGA AGCAATTTTG CCATCATTTA TTTTATCAGT 
TTTTATATGC TTTGTGCATT TTTGATAATA AACTTATTTG TGGCTGTCAT TATGGATAAC 
TTTGACTATT TGACACGTGA CTGGTCCATT CTGGGTCCTC ATCATTTGGA TGAGTTCAAA 
AGGATATGGT CAGAATATGA CCCTGAAGCA AAGGGAAGGA TAAAGCATCT TGATGTGGTT 
ACGTTACTGA GACGCATTCA GCCACCACTT GGTTTTGGCA AATTATGCCC TCACCGGGTG 
GCCTGCAAGA GGTTAGTGGC CATGAATATG CCACTAAATA GTGACGGAAC CGTCATGTTC 
AATGCTACTT TGTTTGCTTT GGTTAGGACT GCTCTAAAGA TAAAAACAGA AGGTAACCTA 
GAGCAAGCAA ATGAAGAACT TAGAGCAGTT ATAAAGAAAA TATGGAAGAA AACTAGCATG 
AAATTACTTG ACCAAGTTGT CCCTCCGGCT GGCGATGATG AGGTAACCGT GGGGAAGTTC 
TATGCCACCT TCCTGATACA GGACTACTTT AGGAAATTCA AGAAACGCAA AGAACAAGGA 
CTGGTGGGGA AATATCCTGC GAAGAATACC ACGATTGCTC TGCAGGCAGG ACTAAGGACA 
CTTCATGATA TTGGCCCTGA AATACGCCGA GCTATATCCT GTGACTTGCA AGATGATGAA 
CCAGAGGAAA ACAATCCAGA CGAGGAGGAG GAAGTTTATA AAAGGAATGG TGCCCTCTTT 
GGCAACCATA TAAACCATAT TAGCAGTGAC AGACGAGATT CATTTCAGCA GATCAACACC 
ACGCACCGTC CCTTACATGT GCAGCGGCCT TCAATTCCAT CTGCAAGTGA TACTGAGAAA 
AATATATATC CTCATACAGG AAATTCTGTG TACCACAATC ATCATAACCA CAACTCAGTA 
GGAAAACAAG TTCCAAACTC AACAAATGCC AATCTCAATA ATGCCAACGT ATCCAAAGTT 
GTTCACGGAA AACACGCTAA TTTTGGAAGT CACGAGCATA GATCTGAAAA TGGTTACCAT 
TCGTACTCTA GAGCTGACCA TGAGAAGCGT AGAAGGCCAA GCAGTAGGAG AACCCGCTAC 
TACGAAACAT ATATTAGGTC TGACTCTGGT GATGGCCGTC GACCAACTAT TTGCCGTGAA 
GAACGTGACA TTCGAGACTA TTGCAATGAT GATCATTATT TGGGAGAGCA GGAATATTAT 
AGTGGAGAAG AATACTATGA GGAAGACAGC ATGTTGTCAG GAAACAGACA TGTGTATGAT 
TACCACTGTA GACATCACTG CCATGACTCA GATTTTGAGA GACCAAAAGG TTACCATCAC 
CCACATGGGT TTTTTGAGGA AGATGATTCA CAGACCTGTT ATGATACGAA AAGATCTCCT 
AGGAGACGGC TGCTACCTCC AACACCAGCA TCAAATAGAC GATCTTCCTT TAATTTTGAA 
TGCCTCCGCC GACAGAGTAG TCAAGATGAT ATACCACTCT CTCCAAATTT CCATCATCGC 
ACTGCTTTAC CACTTCACCT AATGCAACAG CAGGTCATGG CTGTTGCAGG TCTGGATTCC 
AGTAAAGCTC ATAAACATTC ACCAAGTCGT TCCACGCGTT CATGGGCTAC CCCACCTGCA 
ACCCCTCCCA ACAGGGACCA TACCCCATAC TATACACCTC TAATCCAAGT TGACAGGGCT 
GAATCTACAG AACATATGAA TGGTAGCCTG CCTTCCTTGC ACAGGAGCTC CTGGTACACA 
GACGACCCTG ATATCTCCTA CCGAACATTT ACACCAGCCA ATTTAACAGT ACCTAATGAC 
TTTAGGCATA AACACAGTGA CAAACAGAGG AGTGCAGACA GTTTGGTAGA AGCGGTACTT 
ATATCTGAAG GCCTAGGAAG GTATGCAAAG GACCCTAAAT TTGTTTCAGC TACAAAACAT 
GAGATTGCTG ATGCTTGTGA CATGACTATC GATGAGATGG AAAGTGCAGC AAGTAATCTT 
CTCAATGGAA ATATTAGCAA TGGGACCAAT GGGGATATGT TTCCCATTTT AAGCAGACAA 
GATTATGAAC TTCAAGATTT TGGTCCTGGC TACAGTGATG AAGAACCAGA ACCAGGACGA 
TATGAAGAAG ACTTAGCTGA TGAAATGATA TGCATTACAA GCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292991.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148477.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X22, mRNA.

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CloneID	OGa41349
Clone ID Related Accession (Same CDS sequence)	XM_015292991.2 , XM_015292991.1
Accession Version	XM_015292991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148477.1
CDS	335..6853
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292991.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292991.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292991.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148477.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X17, mRNA.

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CloneID	OGa41349
Clone ID Related Accession (Same CDS sequence)	XM_015292991.2 , XM_015292991.1
Accession Version	XM_015292991.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148477.1
CDS	335..6853
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292991.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292991.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC CAAAGCCAAC TGAAACAGTC ACAACTGATG AGTCTGGGAA CTCCGAAGAC 
AGCGCAAGAA TCTCCATCAC TTTTTTCCGT CTTTTCCGAG TGATGAGGTT GGTGAAGCTG 
CTTAGCAGAG GAGAAGGAAT CAGAACTTTA TTGTGGACAT TTATTAAGTC ATTTCAGGCT 
CTGCCTTATG TTGCCCTTCT GATAGCTATG CTGTTCTTCA TCTATGCTGT CATTGGAATG 
CAGGTATTTG GAAAAGTGGC CATGAGAGAC AACAATCAAA TAAACAGAAA CAACAATTTT 
CAAACTTTCC CCCAAGCTGT GCTGCTTCTC TTCAGGTGTG CAACTGGAGA AGCCTGGCAG 
GAAATCATGC TGGCATGTTT GCCTGGAAAA CGCTGTGATC CAGAATCTGA TTATAATCCA 
GGGGAAGAAT ACACATGTGG AAGCAATTTT GCCATCATTT ATTTTATCAG TTTTTATATG 
CTTTGTGCAT TTTTGATAAT AAACTTATTT GTGGCTGTCA TTATGGATAA CTTTGACTAT 
TTGACACGTG ACTGGTCCAT TCTGGGTCCT CATCATTTGG ATGAGTTCAA AAGGATATGG 
TCAGAATATG ACCCTGAAGC AAAGGGAAGG ATAAAGCATC TTGATGTGGT TACGTTACTG 
AGACGCATTC AGCCACCACT TGGTTTTGGC AAATTATGCC CTCACCGGGT GGCCTGCAAG 
AGGTTAGTGG CCATGAATAT GCCACTAAAT AGTGACGGAA CCGTCATGTT CAATGCTACT 
TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG ATAAAAACAG AAGGTAACCT AGAGCAAGCA 
AATGAAGAAC TTAGAGCAGT TATAAAGAAA ATATGGAAGA AAACTAGCAT GAAATTACTT 
GACCAAGTTG TCCCTCCGGC TGGCGATGAT GAGGTAACCG TGGGGAAGTT CTATGCCACC 
TTCCTGATAC AGGACTACTT TAGGAAATTC AAGAAACGCA AAGAACAAGG ACTGGTGGGG 
AAATATCCTG CGAAGAATAC CACGATTGCT CTGCAGGCAG GACTAAGGAC ACTTCATGAT 
ATTGGCCCTG AAATACGCCG AGCTATATCC TGTGACTTGC AAGATGATGA ACCAGAGGAA 
AACAATCCAG ACGAGGAGGA GGAAGTTTAT AAAAGGAATG GTGCCCTCTT TGGCAACCAT 
ATAAACCATA TTAGCAGTGA CAGACGAGAT TCATTTCAGC AGATCAACAC CACGCACCGT 
CCCTTACATG TGCAGCGGCC TTCAATTCCA TCTGCAAGTG ATACTGAGAA AAATATATAT 
CCTCATACAG GAAATTCTGT GTACCACAAT CATCATAACC ACAACTCAGT AGGAAAACAA 
GTTCCAAACT CAACAAATGC CAATCTCAAT AATGCCAACG TATCCAAAGT TGTTCACGGA 
AAACACGCTA ATTTTGGAAG TCACGAGCAT AGATCTGAAA ATGGTTACCA TTCGTACTCT 
AGAGCTGACC ATGAGAAGCG TAGAAGGCCA AGCAGTAGGA GAACCCGCTA CTACGAAACA 
TATATTAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292992.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148478.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X23, mRNA.

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CloneID	OGa41350
Clone ID Related Accession (Same CDS sequence)	XM_015292992.2 , XM_015292992.1
Accession Version	XM_015292992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148478.1
CDS	335..6838
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292992.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292992.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292992.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148478.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X18, mRNA.

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CloneID	OGa41350
Clone ID Related Accession (Same CDS sequence)	XM_015292992.2 , XM_015292992.1
Accession Version	XM_015292992.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148478.1
CDS	335..6838
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292992.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292992.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGG GCTATTTTAG TGATGCCTGG AATACGTTTG ACTCCCTCAT CGTTATTGGC 
AGCATAGTAG ACGTCGTTCT CAGTGAAGCT GATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292993.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148479.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X24, mRNA.

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CloneID	OGa41351
Clone ID Related Accession (Same CDS sequence)	XM_015292993.2 , XM_015292993.1
Accession Version	XM_015292993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148479.1
CDS	335..6838
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292993.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292993.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292993.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148479.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X19, mRNA.

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CloneID	OGa41351
Clone ID Related Accession (Same CDS sequence)	XM_015292993.2 , XM_015292993.1
Accession Version	XM_015292993.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148479.1
CDS	335..6838
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292993.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292993.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATACTT 
GGTTACTTTG ACTATGCTTT CACAGCCATC TTTACTGTTG AAATCCTGTT AAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292994.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148480.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X20, mRNA.

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CloneID	OGa41352
Clone ID Related Accession (Same CDS sequence)	XM_015292994.1 , XM_015292994.2
Accession Version	XM_015292994.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148480.1
CDS	335..6838
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292994.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292994.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292994.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148480.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X25, mRNA.

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CloneID	OGa41352
Clone ID Related Accession (Same CDS sequence)	XM_015292994.1 , XM_015292994.2
Accession Version	XM_015292994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148480.1
CDS	335..6838
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292994.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292994.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACAGAAGTT AATAACTCCG AAGACAGCGC AAGAATCTCC 
ATCACTTTTT TCCGTCTTTT CCGAGTGATG AGGTTGGTGA AGCTGCTTAG CAGAGGAGAA 
GGAATCAGAA CTTTATTGTG GACATTTATT AAGTCATTTC AGGCTCTGCC TTATGTTGCC 
CTTCTGATAG CTATGCTGTT CTTCATCTAT GCTGTCATTG GAATGCAGGT ATTTGGAAAA 
GTGGCCATGA GAGACAACAA TCAAATAAAC AGAAACAACA ATTTTCAAAC TTTCCCCCAA 
GCTGTGCTGC TTCTCTTCAG GTGTGCAACT GGAGAAGCCT GGCAGGAAAT CATGCTGGCA 
TGTTTGCCTG GAAAACGCTG TGATCCAGAA TCTGATTATA ATCCAGGGGA AGAATACACA 
TGTGGAAGCA ATTTTGCCAT CATTTATTTT ATCAGTTTTT ATATGCTTTG TGCATTTTTG 
ATAATAAACT TATTTGTGGC TGTCATTATG GATAACTTTG ACTATTTGAC ACGTGACTGG 
TCCATTCTGG GTCCTCATCA TTTGGATGAG TTCAAAAGGA TATGGTCAGA ATATGACCCT 
GAAGCAAAGG GAAGGATAAA GCATCTTGAT GTGGTTACGT TACTGAGACG CATTCAGCCA 
CCACTTGGTT TTGGCAAATT ATGCCCTCAC CGGGTGGCCT GCAAGAGGTT AGTGGCCATG 
AATATGCCAC TAAATAGTGA CGGAACCGTC ATGTTCAATG CTACTTTGTT TGCTTTGGTT 
AGGACTGCTC TAAAGATAAA AACAGAAGGT AACCTAGAGC AAGCAAATGA AGAACTTAGA 
GCAGTTATAA AGAAAATATG GAAGAAAACT AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCGGCTGGCG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACCTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGCAAAGAA CAAGGACTGG TGGGGAAATA TCCTGCGAAG 
AATACCACGA TTGCTCTGCA GGCAGGACTA AGGACACTTC ATGATATTGG CCCTGAAATA 
CGCCGAGCTA TATCCTGTGA CTTGCAAGAT GATGAACCAG AGGAAAACAA TCCAGACGAG 
GAGGAGGAAG TTTATAAAAG GAATGGTGCC CTCTTTGGCA ACCATATAAA CCATATTAGC 
AGTGACAGAC GAGATTCATT TCAGCAGATC AACACCACGC ACCGTCCCTT ACATGTGCAG 
CGGCCTTCAA TTCCATCTGC AAGTGATACT GAGAAAAATA TATATCCTCA TACAGGAAAT 
TCTGTGTACC ACAATCATCA TAACCACAAC TCAGTAGGAA AACAAGTTCC AAACTCAACA 
AATGCCAATC TCAATAATGC CAACGTATCC AAAGTTGTTC ACGGAAAACA CGCTAATTTT 
GGAAGTCACG AGCATAGATC TGAAAATGGT TACCATTCGT ACTCTAGAGC TGACCATGAG 
AAGCGTAGAA GGCCAAGCAG TAGGAGAACC CGCTACTACG AAACATATAT TAGGTCTGAC 
TCTGGTGATG GCCGTCGACC AACTATTTGC CGTGAAGAAC GTGACATTCG AGACTATTGC 
AATGATGATC ATTATTTGGG AGAGCAGGAA TATTATAGTG GAGAAGAATA CTATGAGGAA 
GACAGCATGT TGTCAGGAAA CAGACATGTG TATGATTACC ACTGTAGACA TCACTGCCAT 
GACTCAGATT TTGAGAGACC AAAAGGTTAC CATCACCCAC ATGGGTTTTT TGAGGAAGAT 
GATTCACAGA CCTGTTATGA TACGAAAAGA TCTCCTAGGA GACGGCTGCT ACCTCCAACA 
CCAGCATCAA ATAGACGATC TTCCTTTAAT TTTGAATGCC TCCGCCGACA GAGTAGTCAA 
GATGATATAC CACTCTCTCC AAATTTCCAT CATCGCACTG CTTTACCACT TCACCTAATG 
CAACAGCAGG TCATGGCTGT TGCAGGTCTG GATTCCAGTA AAGCTCATAA ACATTCACCA 
AGTCGTTCCA CGCGTTCATG GGCTACCCCA CCTGCAACCC CTCCCAACAG GGACCATACC 
CCATACTATA CACCTCTAAT CCAAGTTGAC AGGGCTGAAT CTACAGAACA TATGAATGGT 
AGCCTGCCTT CCTTGCACAG GAGCTCCTGG TACACAGACG ACCCTGATAT CTCCTACCGA 
ACATTTACAC CAGCCAATTT AACAGTACCT AATGACTTTA GGCATAAACA CAGTGACAAA 
CAGAGGAGTG CAGACAGTTT GGTAGAAGCG GTACTTATAT CTGAAGGCCT AGGAAGGTAT 
GCAAAGGACC CTAAATTTGT TTCAGCTACA AAACATGAGA TTGCTGATGC TTGTGACATG 
ACTATCGATG AGATGGAAAG TGCAGCAAGT AATCTTCTCA ATGGAAATAT TAGCAATGGG 
ACCAATGGGG ATATGTTTCC CATTTTAAGC AGACAAGATT ATGAACTTCA AGATTTTGGT 
CCTGGCTACA GTGATGAAGA ACCAGAACCA GGACGATATG AAGAAGACTT AGCTGATGAA 
ATGATATGCA TTACAAGCTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292995.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148481.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X27, mRNA.

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CloneID	OGa41353
Clone ID Related Accession (Same CDS sequence)	XM_015292995.2 , XM_015292995.1
Accession Version	XM_015292995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148481.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292995.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292995.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292995.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148481.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X21, mRNA.

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CloneID	OGa41353
Clone ID Related Accession (Same CDS sequence)	XM_015292995.2 , XM_015292995.1
Accession Version	XM_015292995.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148481.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292995.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292995.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292996.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148482.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X22, mRNA.

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CloneID	OGa41354
Clone ID Related Accession (Same CDS sequence)	XM_015292996.1 , XM_015292996.2
Accession Version	XM_015292996.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148482.1
CDS	335..6733
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292996.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292996.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292996.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148482.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X28, mRNA.

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CloneID	OGa41354
Clone ID Related Accession (Same CDS sequence)	XM_015292996.1 , XM_015292996.2
Accession Version	XM_015292996.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148482.1
CDS	335..6733
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292996.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292996.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGGT CTGACTCTGG TGATGGCCGT CGACCAACTA TTTGCCGTGA AGAACGTGAC 
ATTCGAGACT ATTGCAATGA TGATCATTAT TTGGGAGAGC AGGAATATTA TAGTGGAGAA 
GAATACTATG AGGAAGACAG CATGTTGTCA GGAAACAGAC ATGTGTATGA TTACCACTGT 
AGACATCACT GCCATGACTC AGATTTTGAG AGACCAAAAG GTTACCATCA CCCACATGGG 
TTTTTTGAGG AAGATGATTC ACAGACCTGT TATGATACGA AAAGATCTCC TAGGAGACGG 
CTGCTACCTC CAACACCAGC ATCAAATAGA CGATCTTCCT TTAATTTTGA ATGCCTCCGC 
CGACAGAGTA GTCAAGATGA TATACCACTC TCTCCAAATT TCCATCATCG CACTGCTTTA 
CCACTTCACC TAATGCAACA GCAGGTCATG GCTGTTGCAG GTCTGGATTC CAGTAAAGCT 
CATAAACATT CACCAAGTCG TTCCACGCGT TCATGGGCTA CCCCACCTGC AACCCCTCCC 
AACAGGGACC ATACCCCATA CTATACACCT CTAATCCAAG TTGACAGGGC TGAATCTACA 
GAACATATGA ATGGTAGCCT GCCTTCCTTG CACAGGAGCT CCTGGTACAC AGACGACCCT 
GATATCTCCT ACCGAACATT TACACCAGCC AATTTAACAG TACCTAATGA CTTTAGGCAT 
AAACACAGTG ACAAACAGAG GAGTGCAGAC AGTTTGGTAG AAGCGGTACT TATATCTGAA 
GGCCTAGGAA GGTATGCAAA GGACCCTAAA TTTGTTTCAG CTACAAAACA TGAGATTGCT 
GATGCTTGTG ACATGACTAT CGATGAGATG GAAAGTGCAG CAAGTAATCT TCTCAATGGA 
AATATTAGCA ATGGGACCAA TGGGGATATG TTTCCCATTT TAAGCAGACA AGATTATGAA 
CTTCAAGATT TTGGTCCTGG CTACAGTGAT GAAGAACCAG AACCAGGACG ATATGAAGAA 
GACTTAGCTG ATGAAATGAT ATGCATTACA AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292997.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148483.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X31, mRNA.

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CloneID	OGa41355
Clone ID Related Accession (Same CDS sequence)	XM_015292997.2 , XM_015292997.1
Accession Version	XM_015292997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148483.1
CDS	335..6943
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292997.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292997.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292997.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148483.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X23, mRNA.

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CloneID	OGa41355
Clone ID Related Accession (Same CDS sequence)	XM_015292997.2 , XM_015292997.1
Accession Version	XM_015292997.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148483.1
CDS	335..6943
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292997.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292997.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292998.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148484.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X24, mRNA.

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CloneID	OGa41356
Clone ID Related Accession (Same CDS sequence)	XM_015292998.1 , XM_015292998.2
Accession Version	XM_015292998.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATA ACTCCGAAGA CAGCGCAAGA 
ATCTCCATCA CTTTTTTCCG TCTTTTCCGA GTGATGAGGT TGGTGAAGCT GCTTAGCAGA 
GGAGAAGGAA TCAGAACTTT ATTGTGGACA TTTATTAAGT CATTTCAGGC TCTGCCTTAT 
GTTGCCCTTC TGATAGCTAT GCTGTTCTTC ATCTATGCTG TCATTGGAAT GCAGGTATTT 
GGAAAAGTGG CCATGAGAGA CAACAATCAA ATAAACAGAA ACAACAATTT TCAAACTTTC 
CCCCAAGCTG TGCTGCTTCT CTTCAGGTGT GCAACTGGAG AAGCCTGGCA GGAAATCATG 
CTGGCATGTT TGCCTGGAAA ACGCTGTGAT CCAGAATCTG ATTATAATCC AGGGGAAGAA 
TACACATGTG GAAGCAATTT TGCCATCATT TATTTTATCA GTTTTTATAT GCTTTGTGCA 
TTTTTGATAA TAAACTTATT TGTGGCTGTC ATTATGGATA ACTTTGACTA TTTGACACGT 
GACTGGTCCA TTCTGGGTCC TCATCATTTG GATGAGTTCA AAAGGATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAGCAT CTTGATGTGG TTACGTTACT GAGACGCATT 
CAGCCACCAC TTGGTTTTGG CAAATTATGC CCTCACCGGG TGGCCTGCAA GAGGTTAGTG 
GCCATGAATA TGCCACTAAA TAGTGACGGA ACCGTCATGT TCAATGCTAC TTTGTTTGCT 
TTGGTTAGGA CTGCTCTAAA GATAAAAACA GAAGGTAACC TAGAGCAAGC AAATGAAGAA 
CTTAGAGCAG TTATAAAGAA AATATGGAAG AAAACTAGCA TGAAATTACT TGACCAAGTT 
GTCCCTCCGG CTGGCGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC CTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAACAAG GACTGGTGGG GAAATATCCT 
GCGAAGAATA CCACGATTGC TCTGCAGGCA GGACTAAGGA CACTTCATGA TATTGGCCCT 
GAAATACGCC GAGCTATATC CTGTGACTTG CAAGATGATG AACCAGAGGA AAACAATCCA 
GACGAGGAGG AGGAAGTTTA TAAAAGGAAT GGTGCCCTCT TTGGCAACCA TATAAACCAT 
ATTAGCAGTG ACAGACGAGA TTCATTTCAG CAGATCAACA CCACGCACCG TCCCTTACAT 
GTGCAGCGGC CTTCAATTCC ATCTGCAAGT GATACTGAGA AAAATATATA TCCTCATACA 
GGAAATTCTG TGTACCACAA TCATCATAAC CACAACTCAG TAGGAAAACA AGTTCCAAAC 
TCAACAAATG CCAATCTCAA TAATGCCAAC GTATCCAAAG TTGTTCACGG AAAACACGCT 
AATTTTGGAA GTCACGAGCA TAGATCTGAA AATGGTTACC ATTCGTACTC TAGAGCTGAC 
CATGAGAAGC GTAGAAGGCC AAGCAGTAGG AGAACCCGCT ACTACGAAAC ATATATTAGG 
TCTGACTCTG GTGATGGCCG TCGACCAACT ATTTGCCGTG AAGAACGTGA CATTCGAGAC 
TATTGCAATG ATGATCATTA TTTGGGAGAG CAGGAATATT ATAGTGGAGA AGAATACTAT 
GAGGAAGACA GCATGTTGTC AGGAAACAGA CATGTGTATG ATTACCACTG TAGACATCAC 
TGCCATGACT CAGATTTTGA GAGACCAAAA GGTTACCATC ACCCACATGG GTTTTTTGAG 
GAAGATGATT CACAGACCTG TTATGATACG AAAAGATCTC CTAGGAGACG GCTGCTACCT 
CCAACACCAG CATCAAATAG ACGATCTTCC TTTAATTTTG AATGCCTCCG CCGACAGAGT 
AGTCAAGATG ATATACCACT CTCTCCAAAT TTCCATCATC GCACTGCTTT ACCACTTCAC 
CTAATGCAAC AGCAGGTCAT GGCTGTTGCA GGTCTGGATT CCAGTAAAGC TCATAAACAT 
TCACCAAGTC GTTCCACGCG TTCATGGGCT ACCCCACCTG CAACCCCTCC CAACAGGGAC 
CATACCCCAT ACTATACACC TCTAATCCAA GTTGACAGGG CTGAATCTAC AGAACATATG 
AATGGTAGCC TGCCTTCCTT GCACAGGAGC TCCTGGTACA CAGACGACCC TGATATCTCC 
TACCGAACAT TTACACCAGC CAATTTAACA GTACCTAATG ACTTTAGGCA TAAACACAGT 
GACAAACAGA GGAGTGCAGA CAGTTTGGTA GAAGCGGTAC TTATATCTGA AGGCCTAGGA 
AGGTATGCAA AGGACCCTAA ATTTGTTTCA GCTACAAAAC ATGAGATTGC TGATGCTTGT 
GACATGACTA TCGATGAGAT GGAAAGTGCA GCAAGTAATC TTCTCAATGG AAATATTAGC 
AATGGGACCA ATGGGGATAT GTTTCCCATT TTAAGCAGAC AAGATTATGA ACTTCAAGAT 
TTTGGTCCTG GCTACAGTGA TGAAGAACCA GAACCAGGAC GATATGAAGA AGACTTAGCT 
GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148484.1
CDS	335..6844
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292998.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292998.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATA ACTCCGAAGA CAGCGCAAGA 
ATCTCCATCA CTTTTTTCCG TCTTTTCCGA GTGATGAGGT TGGTGAAGCT GCTTAGCAGA 
GGAGAAGGAA TCAGAACTTT ATTGTGGACA TTTATTAAGT CATTTCAGGC TCTGCCTTAT 
GTTGCCCTTC TGATAGCTAT GCTGTTCTTC ATCTATGCTG TCATTGGAAT GCAGGTATTT 
GGAAAAGTGG CCATGAGAGA CAACAATCAA ATAAACAGAA ACAACAATTT TCAAACTTTC 
CCCCAAGCTG TGCTGCTTCT CTTCAGGTGT GCAACTGGAG AAGCCTGGCA GGAAATCATG 
CTGGCATGTT TGCCTGGAAA ACGCTGTGAT CCAGAATCTG ATTATAATCC AGGGGAAGAA 
TACACATGTG GAAGCAATTT TGCCATCATT TATTTTATCA GTTTTTATAT GCTTTGTGCA 
TTTTTGATAA TAAACTTATT TGTGGCTGTC ATTATGGATA ACTTTGACTA TTTGACACGT 
GACTGGTCCA TTCTGGGTCC TCATCATTTG GATGAGTTCA AAAGGATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAGCAT CTTGATGTGG TTACGTTACT GAGACGCATT 
CAGCCACCAC TTGGTTTTGG CAAATTATGC CCTCACCGGG TGGCCTGCAA GAGGTTAGTG 
GCCATGAATA TGCCACTAAA TAGTGACGGA ACCGTCATGT TCAATGCTAC TTTGTTTGCT 
TTGGTTAGGA CTGCTCTAAA GATAAAAACA GAAGGTAACC TAGAGCAAGC AAATGAAGAA 
CTTAGAGCAG TTATAAAGAA AATATGGAAG AAAACTAGCA TGAAATTACT TGACCAAGTT 
GTCCCTCCGG CTGGCGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC CTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAACAAG GACTGGTGGG GAAATATCCT 
GCGAAGAATA CCACGATTGC TCTGCAGGCA GGACTAAGGA CACTTCATGA TATTGGCCCT 
GAAATACGCC GAGCTATATC CTGTGACTTG CAAGATGATG AACCAGAGGA AAACAATCCA 
GACGAGGAGG AGGAAGTTTA TAAAAGGAAT GGTGCCCTCT TTGGCAACCA TATAAACCAT 
ATTAGCAGTG ACAGACGAGA TTCATTTCAG CAGATCAACA CCACGCACCG TCCCTTACAT 
GTGCAGCGGC CTTCAATTCC ATCTGCAAGT GATACTGAGA AAAATATATA TCCTCATACA 
GGAAATTCTG TGTACCACAA TCATCATAAC CACAACTCAG TAGGAAAACA AGTTCCAAAC 
TCAACAAATG CCAATCTCAA TAATGCCAAC GTATCCAAAG TTGTTCACGG AAAACACGCT 
AATTTTGGAA GTCACGAGCA TAGATCTGAA AATGGTTACC ATTCGTACTC TAGAGCTGAC 
CATGAGAAGC GTAGAAGGCC AAGCAGTAGG AGAACCCGCT ACTACGAAAC ATATATTAGG 
TCTGACTCTG GTGATGGCCG TCGACCAACT ATTTGCCGTG AAGAACGTGA CATTCGAGAC 
TATTGCAATG ATGATCATTA TTTGGGAGAG CAGGAATATT ATAGTGGAGA AGAATACTAT 
GAGGAAGACA GCATGTTGTC AGGAAACAGA CATGTGTATG ATTACCACTG TAGACATCAC 
TGCCATGACT CAGATTTTGA GAGACCAAAA GGTTACCATC ACCCACATGG GTTTTTTGAG 
GAAGATGATT CACAGACCTG TTATGATACG AAAAGATCTC CTAGGAGACG GCTGCTACCT 
CCAACACCAG CATCAAATAG ACGATCTTCC TTTAATTTTG AATGCCTCCG CCGACAGAGT 
AGTCAAGATG ATATACCACT CTCTCCAAAT TTCCATCATC GCACTGCTTT ACCACTTCAC 
CTAATGCAAC AGCAGGTCAT GGCTGTTGCA GGTCTGGATT CCAGTAAAGC TCATAAACAT 
TCACCAAGTC GTTCCACGCG TTCATGGGCT ACCCCACCTG CAACCCCTCC CAACAGGGAC 
CATACCCCAT ACTATACACC TCTAATCCAA GTTGACAGGG CTGAATCTAC AGAACATATG 
AATGGTAGCC TGCCTTCCTT GCACAGGAGC TCCTGGTACA CAGACGACCC TGATATCTCC 
TACCGAACAT TTACACCAGC CAATTTAACA GTACCTAATG ACTTTAGGCA TAAACACAGT 
GACAAACAGA GGAGTGCAGA CAGTTTGGTA GAAGCGGTAC TTATATCTGA AGGCCTAGGA 
AGGTATGCAA AGGACCCTAA ATTTGTTTCA GCTACAAAAC ATGAGATTGC TGATGCTTGT 
GACATGACTA TCGATGAGAT GGAAAGTGCA GCAAGTAATC TTCTCAATGG AAATATTAGC 
AATGGGACCA ATGGGGATAT GTTTCCCATT TTAAGCAGAC AAGATTATGA ACTTCAAGAT 
TTTGGTCCTG GCTACAGTGA TGAAGAACCA GAACCAGGAC GATATGAAGA AGACTTAGCT 
GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292998.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148484.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X32, mRNA.

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CloneID	OGa41356
Clone ID Related Accession (Same CDS sequence)	XM_015292998.1 , XM_015292998.2
Accession Version	XM_015292998.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATA ACTCCGAAGA CAGCGCAAGA 
ATCTCCATCA CTTTTTTCCG TCTTTTCCGA GTGATGAGGT TGGTGAAGCT GCTTAGCAGA 
GGAGAAGGAA TCAGAACTTT ATTGTGGACA TTTATTAAGT CATTTCAGGC TCTGCCTTAT 
GTTGCCCTTC TGATAGCTAT GCTGTTCTTC ATCTATGCTG TCATTGGAAT GCAGGTATTT 
GGAAAAGTGG CCATGAGAGA CAACAATCAA ATAAACAGAA ACAACAATTT TCAAACTTTC 
CCCCAAGCTG TGCTGCTTCT CTTCAGGTGT GCAACTGGAG AAGCCTGGCA GGAAATCATG 
CTGGCATGTT TGCCTGGAAA ACGCTGTGAT CCAGAATCTG ATTATAATCC AGGGGAAGAA 
TACACATGTG GAAGCAATTT TGCCATCATT TATTTTATCA GTTTTTATAT GCTTTGTGCA 
TTTTTGATAA TAAACTTATT TGTGGCTGTC ATTATGGATA ACTTTGACTA TTTGACACGT 
GACTGGTCCA TTCTGGGTCC TCATCATTTG GATGAGTTCA AAAGGATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAGCAT CTTGATGTGG TTACGTTACT GAGACGCATT 
CAGCCACCAC TTGGTTTTGG CAAATTATGC CCTCACCGGG TGGCCTGCAA GAGGTTAGTG 
GCCATGAATA TGCCACTAAA TAGTGACGGA ACCGTCATGT TCAATGCTAC TTTGTTTGCT 
TTGGTTAGGA CTGCTCTAAA GATAAAAACA GAAGGTAACC TAGAGCAAGC AAATGAAGAA 
CTTAGAGCAG TTATAAAGAA AATATGGAAG AAAACTAGCA TGAAATTACT TGACCAAGTT 
GTCCCTCCGG CTGGCGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC CTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAACAAG GACTGGTGGG GAAATATCCT 
GCGAAGAATA CCACGATTGC TCTGCAGGCA GGACTAAGGA CACTTCATGA TATTGGCCCT 
GAAATACGCC GAGCTATATC CTGTGACTTG CAAGATGATG AACCAGAGGA AAACAATCCA 
GACGAGGAGG AGGAAGTTTA TAAAAGGAAT GGTGCCCTCT TTGGCAACCA TATAAACCAT 
ATTAGCAGTG ACAGACGAGA TTCATTTCAG CAGATCAACA CCACGCACCG TCCCTTACAT 
GTGCAGCGGC CTTCAATTCC ATCTGCAAGT GATACTGAGA AAAATATATA TCCTCATACA 
GGAAATTCTG TGTACCACAA TCATCATAAC CACAACTCAG TAGGAAAACA AGTTCCAAAC 
TCAACAAATG CCAATCTCAA TAATGCCAAC GTATCCAAAG TTGTTCACGG AAAACACGCT 
AATTTTGGAA GTCACGAGCA TAGATCTGAA AATGGTTACC ATTCGTACTC TAGAGCTGAC 
CATGAGAAGC GTAGAAGGCC AAGCAGTAGG AGAACCCGCT ACTACGAAAC ATATATTAGG 
TCTGACTCTG GTGATGGCCG TCGACCAACT ATTTGCCGTG AAGAACGTGA CATTCGAGAC 
TATTGCAATG ATGATCATTA TTTGGGAGAG CAGGAATATT ATAGTGGAGA AGAATACTAT 
GAGGAAGACA GCATGTTGTC AGGAAACAGA CATGTGTATG ATTACCACTG TAGACATCAC 
TGCCATGACT CAGATTTTGA GAGACCAAAA GGTTACCATC ACCCACATGG GTTTTTTGAG 
GAAGATGATT CACAGACCTG TTATGATACG AAAAGATCTC CTAGGAGACG GCTGCTACCT 
CCAACACCAG CATCAAATAG ACGATCTTCC TTTAATTTTG AATGCCTCCG CCGACAGAGT 
AGTCAAGATG ATATACCACT CTCTCCAAAT TTCCATCATC GCACTGCTTT ACCACTTCAC 
CTAATGCAAC AGCAGGTCAT GGCTGTTGCA GGTCTGGATT CCAGTAAAGC TCATAAACAT 
TCACCAAGTC GTTCCACGCG TTCATGGGCT ACCCCACCTG CAACCCCTCC CAACAGGGAC 
CATACCCCAT ACTATACACC TCTAATCCAA GTTGACAGGG CTGAATCTAC AGAACATATG 
AATGGTAGCC TGCCTTCCTT GCACAGGAGC TCCTGGTACA CAGACGACCC TGATATCTCC 
TACCGAACAT TTACACCAGC CAATTTAACA GTACCTAATG ACTTTAGGCA TAAACACAGT 
GACAAACAGA GGAGTGCAGA CAGTTTGGTA GAAGCGGTAC TTATATCTGA AGGCCTAGGA 
AGGTATGCAA AGGACCCTAA ATTTGTTTCA GCTACAAAAC ATGAGATTGC TGATGCTTGT 
GACATGACTA TCGATGAGAT GGAAAGTGCA GCAAGTAATC TTCTCAATGG AAATATTAGC 
AATGGGACCA ATGGGGATAT GTTTCCCATT TTAAGCAGAC AAGATTATGA ACTTCAAGAT 
TTTGGTCCTG GCTACAGTGA TGAAGAACCA GAACCAGGAC GATATGAAGA AGACTTAGCT 
GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148484.1
CDS	335..6844
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292998.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292998.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATA ACTCCGAAGA CAGCGCAAGA 
ATCTCCATCA CTTTTTTCCG TCTTTTCCGA GTGATGAGGT TGGTGAAGCT GCTTAGCAGA 
GGAGAAGGAA TCAGAACTTT ATTGTGGACA TTTATTAAGT CATTTCAGGC TCTGCCTTAT 
GTTGCCCTTC TGATAGCTAT GCTGTTCTTC ATCTATGCTG TCATTGGAAT GCAGGTATTT 
GGAAAAGTGG CCATGAGAGA CAACAATCAA ATAAACAGAA ACAACAATTT TCAAACTTTC 
CCCCAAGCTG TGCTGCTTCT CTTCAGGTGT GCAACTGGAG AAGCCTGGCA GGAAATCATG 
CTGGCATGTT TGCCTGGAAA ACGCTGTGAT CCAGAATCTG ATTATAATCC AGGGGAAGAA 
TACACATGTG GAAGCAATTT TGCCATCATT TATTTTATCA GTTTTTATAT GCTTTGTGCA 
TTTTTGATAA TAAACTTATT TGTGGCTGTC ATTATGGATA ACTTTGACTA TTTGACACGT 
GACTGGTCCA TTCTGGGTCC TCATCATTTG GATGAGTTCA AAAGGATATG GTCAGAATAT 
GACCCTGAAG CAAAGGGAAG GATAAAGCAT CTTGATGTGG TTACGTTACT GAGACGCATT 
CAGCCACCAC TTGGTTTTGG CAAATTATGC CCTCACCGGG TGGCCTGCAA GAGGTTAGTG 
GCCATGAATA TGCCACTAAA TAGTGACGGA ACCGTCATGT TCAATGCTAC TTTGTTTGCT 
TTGGTTAGGA CTGCTCTAAA GATAAAAACA GAAGGTAACC TAGAGCAAGC AAATGAAGAA 
CTTAGAGCAG TTATAAAGAA AATATGGAAG AAAACTAGCA TGAAATTACT TGACCAAGTT 
GTCCCTCCGG CTGGCGATGA TGAGGTAACC GTGGGGAAGT TCTATGCCAC CTTCCTGATA 
CAGGACTACT TTAGGAAATT CAAGAAACGC AAAGAACAAG GACTGGTGGG GAAATATCCT 
GCGAAGAATA CCACGATTGC TCTGCAGGCA GGACTAAGGA CACTTCATGA TATTGGCCCT 
GAAATACGCC GAGCTATATC CTGTGACTTG CAAGATGATG AACCAGAGGA AAACAATCCA 
GACGAGGAGG AGGAAGTTTA TAAAAGGAAT GGTGCCCTCT TTGGCAACCA TATAAACCAT 
ATTAGCAGTG ACAGACGAGA TTCATTTCAG CAGATCAACA CCACGCACCG TCCCTTACAT 
GTGCAGCGGC CTTCAATTCC ATCTGCAAGT GATACTGAGA AAAATATATA TCCTCATACA 
GGAAATTCTG TGTACCACAA TCATCATAAC CACAACTCAG TAGGAAAACA AGTTCCAAAC 
TCAACAAATG CCAATCTCAA TAATGCCAAC GTATCCAAAG TTGTTCACGG AAAACACGCT 
AATTTTGGAA GTCACGAGCA TAGATCTGAA AATGGTTACC ATTCGTACTC TAGAGCTGAC 
CATGAGAAGC GTAGAAGGCC AAGCAGTAGG AGAACCCGCT ACTACGAAAC ATATATTAGG 
TCTGACTCTG GTGATGGCCG TCGACCAACT ATTTGCCGTG AAGAACGTGA CATTCGAGAC 
TATTGCAATG ATGATCATTA TTTGGGAGAG CAGGAATATT ATAGTGGAGA AGAATACTAT 
GAGGAAGACA GCATGTTGTC AGGAAACAGA CATGTGTATG ATTACCACTG TAGACATCAC 
TGCCATGACT CAGATTTTGA GAGACCAAAA GGTTACCATC ACCCACATGG GTTTTTTGAG 
GAAGATGATT CACAGACCTG TTATGATACG AAAAGATCTC CTAGGAGACG GCTGCTACCT 
CCAACACCAG CATCAAATAG ACGATCTTCC TTTAATTTTG AATGCCTCCG CCGACAGAGT 
AGTCAAGATG ATATACCACT CTCTCCAAAT TTCCATCATC GCACTGCTTT ACCACTTCAC 
CTAATGCAAC AGCAGGTCAT GGCTGTTGCA GGTCTGGATT CCAGTAAAGC TCATAAACAT 
TCACCAAGTC GTTCCACGCG TTCATGGGCT ACCCCACCTG CAACCCCTCC CAACAGGGAC 
CATACCCCAT ACTATACACC TCTAATCCAA GTTGACAGGG CTGAATCTAC AGAACATATG 
AATGGTAGCC TGCCTTCCTT GCACAGGAGC TCCTGGTACA CAGACGACCC TGATATCTCC 
TACCGAACAT TTACACCAGC CAATTTAACA GTACCTAATG ACTTTAGGCA TAAACACAGT 
GACAAACAGA GGAGTGCAGA CAGTTTGGTA GAAGCGGTAC TTATATCTGA AGGCCTAGGA 
AGGTATGCAA AGGACCCTAA ATTTGTTTCA GCTACAAAAC ATGAGATTGC TGATGCTTGT 
GACATGACTA TCGATGAGAT GGAAAGTGCA GCAAGTAATC TTCTCAATGG AAATATTAGC 
AATGGGACCA ATGGGGATAT GTTTCCCATT TTAAGCAGAC AAGATTATGA ACTTCAAGAT 
TTTGGTCCTG GCTACAGTGA TGAAGAACCA GAACCAGGAC GATATGAAGA AGACTTAGCT 
GATGAAATGA TATGCATTAC AAGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292999.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148485.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X17, mRNA.

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CloneID	OGa41357
Clone ID Related Accession (Same CDS sequence)	XM_015292999.2 , XM_015292999.1
Accession Version	XM_015292999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015292999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148485.1
CDS	335..6883
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292999.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292999.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015292999.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148485.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X25, mRNA.

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CloneID	OGa41357
Clone ID Related Accession (Same CDS sequence)	XM_015292999.2 , XM_015292999.1
Accession Version	XM_015292999.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148485.1
CDS	335..6883
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKGYFSDAWNTFDSLIVIGSIVDVVLSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015292999.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292999.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GGGCTATTTT 
AGTGATGCCT GGAATACGTT TGACTCCCTC ATCGTTATTG GCAGCATAGT AGACGTCGTT 
CTCAGTGAAG CTGATCACTA TTTCACTGAT GCATGGAACA CTTTTGATGC CTTAATTGTT 
GTTGGTAGCG TCGTTGATAT TGCTATAACA GAAGTTAATC CAAAGCCAAC TGAAACAGTC 
ACAACTGATG AGTCTGGGAA CTCCGAAGAC AGCGCAAGAA TCTCCATCAC TTTTTTCCGT 
CTTTTCCGAG TGATGAGGTT GGTGAAGCTG CTTAGCAGAG GAGAAGGAAT CAGAACTTTA 
TTGTGGACAT TTATTAAGTC ATTTCAGGCT CTGCCTTATG TTGCCCTTCT GATAGCTATG 
CTGTTCTTCA TCTATGCTGT CATTGGAATG CAGGTATTTG GAAAAGTGGC CATGAGAGAC 
AACAATCAAA TAAACAGAAA CAACAATTTT CAAACTTTCC CCCAAGCTGT GCTGCTTCTC 
TTCAGGTGTG CAACTGGAGA AGCCTGGCAG GAAATCATGC TGGCATGTTT GCCTGGAAAA 
CGCTGTGATC CAGAATCTGA TTATAATCCA GGGGAAGAAT ACACATGTGG AAGCAATTTT 
GCCATCATTT ATTTTATCAG TTTTTATATG CTTTGTGCAT TTTTGATAAT AAACTTATTT 
GTGGCTGTCA TTATGGATAA CTTTGACTAT TTGACACGTG ACTGGTCCAT TCTGGGTCCT 
CATCATTTGG ATGAGTTCAA AAGGATATGG TCAGAATATG ACCCTGAAGC AAAGGGAAGG 
ATAAAGCATC TTGATGTGGT TACGTTACTG AGACGCATTC AGCCACCACT TGGTTTTGGC 
AAATTATGCC CTCACCGGGT GGCCTGCAAG AGGTTAGTGG CCATGAATAT GCCACTAAAT 
AGTGACGGAA CCGTCATGTT CAATGCTACT TTGTTTGCTT TGGTTAGGAC TGCTCTAAAG 
ATAAAAACAG AAGGTAACCT AGAGCAAGCA AATGAAGAAC TTAGAGCAGT TATAAAGAAA 
ATATGGAAGA AAACTAGCAT GAAATTACTT GACCAAGTTG TCCCTCCGGC TGGCGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACC TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGCA AAGAACAAGG ACTGGTGGGG AAATATCCTG CGAAGAATAC CACGATTGCT 
CTGCAGGCAG GACTAAGGAC ACTTCATGAT ATTGGCCCTG AAATACGCCG AGCTATATCC 
TGTGACTTGC AAGATGATGA ACCAGAGGAA AACAATCCAG ACGAGGAGGA GGAAGTTTAT 
AAAAGGAATG GTGCCCTCTT TGGCAACCAT ATAAACCATA TTAGCAGTGA CAGACGAGAT 
TCATTTCAGC AGATCAACAC CACGCACCGT CCCTTACATG TGCAGCGGCC TTCAATTCCA 
TCTGCAAGTG ATACTGAGAA AAATATATAT CCTCATACAG GAAATTCTGT GTACCACAAT 
CATCATAACC ACAACTCAGT AGGAAAACAA GTTCCAAACT CAACAAATGC CAATCTCAAT 
AATGCCAACG TATCCAAAGT TGTTCACGGA AAACACGCTA ATTTTGGAAG TCACGAGCAT 
AGATCTGAAA ATGGTTACCA TTCGTACTCT AGAGCTGACC ATGAGAAGCG TAGAAGGCCA 
AGCAGTAGGA GAACCCGCTA CTACGAAACA TATATTAGGT CTGACTCTGG TGATGGCCGT 
CGACCAACTA TTTGCCGTGA AGAACGTGAC ATTCGAGACT ATTGCAATGA TGATCATTAT 
TTGGGAGAGC AGGAATATTA TAGTGGAGAA GAATACTATG AGGAAGACAG CATGTTGTCA 
GGAAACAGAC ATGTGTATGA TTACCACTGT AGACATCACT GCCATGACTC AGATTTTGAG 
AGACCAAAAG GTTACCATCA CCCACATGGG TTTTTTGAGG AAGATGATTC ACAGACCTGT 
TATGATACGA AAAGATCTCC TAGGAGACGG CTGCTACCTC CAACACCAGC ATCAAATAGA 
CGATCTTCCT TTAATTTTGA ATGCCTCCGC CGACAGAGTA GTCAAGATGA TATACCACTC 
TCTCCAAATT TCCATCATCG CACTGCTTTA CCACTTCACC TAATGCAACA GCAGGTCATG 
GCTGTTGCAG GTCTGGATTC CAGTAAAGCT CATAAACATT CACCAAGTCG TTCCACGCGT 
TCATGGGCTA CCCCACCTGC AACCCCTCCC AACAGGGACC ATACCCCATA CTATACACCT 
CTAATCCAAG TTGACAGGGC TGAATCTACA GAACATATGA ATGGTAGCCT GCCTTCCTTG 
CACAGGAGCT CCTGGTACAC AGACGACCCT GATATCTCCT ACCGAACATT TACACCAGCC 
AATTTAACAG TACCTAATGA CTTTAGGCAT AAACACAGTG ACAAACAGAG GAGTGCAGAC 
AGTTTGGTAG AAGCGGTACT TATATCTGAA GGCCTAGGAA GGTATGCAAA GGACCCTAAA 
TTTGTTTCAG CTACAAAACA TGAGATTGCT GATGCTTGTG ACATGACTAT CGATGAGATG 
GAAAGTGCAG CAAGTAATCT TCTCAATGGA AATATTAGCA ATGGGACCAA TGGGGATATG 
TTTCCCATTT TAAGCAGACA AGATTATGAA CTTCAAGATT TTGGTCCTGG CTACAGTGAT 
GAAGAACCAG AACCAGGACG ATATGAAGAA GACTTAGCTG ATGAAATGAT ATGCATTACA 
AGCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293000.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148486.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X26, mRNA.

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CloneID	OGa41358
Clone ID Related Accession (Same CDS sequence)	XM_015293000.1 , XM_015293000.2
Accession Version	XM_015293000.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148486.1
CDS	335..6859
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293000.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293000.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293000.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148486.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X33, mRNA.

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CloneID	OGa41358
Clone ID Related Accession (Same CDS sequence)	XM_015293000.1 , XM_015293000.2
Accession Version	XM_015293000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148486.1
CDS	335..6859
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293000.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293000.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293001.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148487.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X27, mRNA.

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CloneID	OGa41359
Clone ID Related Accession (Same CDS sequence)	XM_015293001.1 , XM_015293001.2
Accession Version	XM_015293001.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148487.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293001.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293001.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293001.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148487.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X34, mRNA.

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CloneID	OGa41359
Clone ID Related Accession (Same CDS sequence)	XM_015293001.1 , XM_015293001.2
Accession Version	XM_015293001.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148487.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293001.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293001.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293002.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148488.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X28, mRNA.

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CloneID	OGa41360
Clone ID Related Accession (Same CDS sequence)	XM_015293002.1 , XM_015293002.2
Accession Version	XM_015293002.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148488.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293002.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293002.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293002.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148488.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X35, mRNA.

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CloneID	OGa41360
Clone ID Related Accession (Same CDS sequence)	XM_015293002.1 , XM_015293002.2
Accession Version	XM_015293002.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148488.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293002.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293002.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293003.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148489.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X29, mRNA.

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CloneID	OGa41361
Clone ID Related Accession (Same CDS sequence)	XM_015293003.1 , XM_015293003.2
Accession Version	XM_015293003.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148489.1
CDS	335..6799
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293003.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293003.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293003.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148489.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X36, mRNA.

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CloneID	OGa41361
Clone ID Related Accession (Same CDS sequence)	XM_015293003.1 , XM_015293003.2
Accession Version	XM_015293003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148489.1
CDS	335..6799
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293003.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293003.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293004.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148490.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X37, mRNA.

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CloneID	OGa41362
Clone ID Related Accession (Same CDS sequence)	XM_015293004.2 , XM_015293004.1
Accession Version	XM_015293004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148490.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293004.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293004.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293004.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148490.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X30, mRNA.

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CloneID	OGa41362
Clone ID Related Accession (Same CDS sequence)	XM_015293004.2 , XM_015293004.1
Accession Version	XM_015293004.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148490.1
CDS	335..6760
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYFDYAFTAIFTVEILLKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293004.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293004.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATAC TTGGTTACTT TGACTATGCT 
TTCACAGCCA TCTTTACTGT TGAAATCCTG TTAAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATAACTC CGAAGACAGC GCAAGAATCT CCATCACTTT TTTCCGTCTT 
TTCCGAGTGA TGAGGTTGGT GAAGCTGCTT AGCAGAGGAG AAGGAATCAG AACTTTATTG 
TGGACATTTA TTAAGTCATT TCAGGCTCTG CCTTATGTTG CCCTTCTGAT AGCTATGCTG 
TTCTTCATCT ATGCTGTCAT TGGAATGCAG GTATTTGGAA AAGTGGCCAT GAGAGACAAC 
AATCAAATAA ACAGAAACAA CAATTTTCAA ACTTTCCCCC AAGCTGTGCT GCTTCTCTTC 
AGGTGTGCAA CTGGAGAAGC CTGGCAGGAA ATCATGCTGG CATGTTTGCC TGGAAAACGC 
TGTGATCCAG AATCTGATTA TAATCCAGGG GAAGAATACA CATGTGGAAG CAATTTTGCC 
ATCATTTATT TTATCAGTTT TTATATGCTT TGTGCATTTT TGATAATAAA CTTATTTGTG 
GCTGTCATTA TGGATAACTT TGACTATTTG ACACGTGACT GGTCCATTCT GGGTCCTCAT 
CATTTGGATG AGTTCAAAAG GATATGGTCA GAATATGACC CTGAAGCAAA GGGAAGGATA 
AAGCATCTTG ATGTGGTTAC GTTACTGAGA CGCATTCAGC CACCACTTGG TTTTGGCAAA 
TTATGCCCTC ACCGGGTGGC CTGCAAGAGG TTAGTGGCCA TGAATATGCC ACTAAATAGT 
GACGGAACCG TCATGTTCAA TGCTACTTTG TTTGCTTTGG TTAGGACTGC TCTAAAGATA 
AAAACAGAAG GTAACCTAGA GCAAGCAAAT GAAGAACTTA GAGCAGTTAT AAAGAAAATA 
TGGAAGAAAA CTAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCGGCTGG CGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACCTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGCAAAG AACAAGGACT GGTGGGGAAA TATCCTGCGA AGAATACCAC GATTGCTCTG 
CAGGCAGGAC TAAGGACACT TCATGATATT GGCCCTGAAA TACGCCGAGC TATATCCTGT 
GACTTGCAAG ATGATGAACC AGAGGAAAAC AATCCAGACG AGGAGGAGGA AGTTTATAAA 
AGGAATGGTG CCCTCTTTGG CAACCATATA AACCATATTA GCAGTGACAG ACGAGATTCA 
TTTCAGCAGA TCAACACCAC GCACCGTCCC TTACATGTGC AGCGGCCTTC AATTCCATCT 
GCAAGTGATA CTGAGAAAAA TATATATCCT CATACAGGAA ATTCTGTGTA CCACAATCAT 
CATAACCACA ACTCAGTAGG AAAACAAGTT CCAAACTCAA CAAATGCCAA TCTCAATAAT 
GCCAACGTAT CCAAAGTTGT TCACGGAAAA CACGCTAATT TTGGAAGTCA CGAGCATAGA 
TCTGAAAATG GTTACCATTC GTACTCTAGA GCTGACCATG AGAAGCGTAG AAGGCCAAGC 
AGTAGGAGAA CCCGCTACTA CGAAACATAT ATTAGGTCTG ACTCTGGTGA TGGCCGTCGA 
CCAACTATTT GCCGTGAAGA ACGTGACATT CGAGACTATT GCAATGATGA TCATTATTTG 
GGAGAGCAGG AATATTATAG TGGAGAAGAA TACTATGAGG AAGACAGCAT GTTGTCAGGA 
AACAGACATG TGTATGATTA CCACTGTAGA CATCACTGCC ATGACTCAGA TTTTGAGAGA 
CCAAAAGGTT ACCATCACCC ACATGGGTTT TTTGAGGAAG ATGATTCACA GACCTGTTAT 
GATACGAAAA GATCTCCTAG GAGACGGCTG CTACCTCCAA CACCAGCATC AAATAGACGA 
TCTTCCTTTA ATTTTGAATG CCTCCGCCGA CAGAGTAGTC AAGATGATAT ACCACTCTCT 
CCAAATTTCC ATCATCGCAC TGCTTTACCA CTTCACCTAA TGCAACAGCA GGTCATGGCT 
GTTGCAGGTC TGGATTCCAG TAAAGCTCAT AAACATTCAC CAAGTCGTTC CACGCGTTCA 
TGGGCTACCC CACCTGCAAC CCCTCCCAAC AGGGACCATA CCCCATACTA TACACCTCTA 
ATCCAAGTTG ACAGGGCTGA ATCTACAGAA CATATGAATG GTAGCCTGCC TTCCTTGCAC 
AGGAGCTCCT GGTACACAGA CGACCCTGAT ATCTCCTACC GAACATTTAC ACCAGCCAAT 
TTAACAGTAC CTAATGACTT TAGGCATAAA CACAGTGACA AACAGAGGAG TGCAGACAGT 
TTGGTAGAAG CGGTACTTAT ATCTGAAGGC CTAGGAAGGT ATGCAAAGGA CCCTAAATTT 
GTTTCAGCTA CAAAACATGA GATTGCTGAT GCTTGTGACA TGACTATCGA TGAGATGGAA 
AGTGCAGCAA GTAATCTTCT CAATGGAAAT ATTAGCAATG GGACCAATGG GGATATGTTT 
CCCATTTTAA GCAGACAAGA TTATGAACTT CAAGATTTTG GTCCTGGCTA CAGTGATGAA 
GAACCAGAAC CAGGACGATA TGAAGAAGAC TTAGCTGATG AAATGATATG CATTACAAGC 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293005.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148491.1 Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X31, mRNA.

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CloneID	OGa41363
Clone ID Related Accession (Same CDS sequence)	XM_015293005.1 , XM_015293005.2
Accession Version	XM_015293005.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148491.1
CDS	335..6799
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293005.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293005.1

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_015293005.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148491.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X38, mRNA.

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CloneID	OGa41363
Clone ID Related Accession (Same CDS sequence)	XM_015293005.1 , XM_015293005.2
Accession Version	XM_015293005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148491.1
CDS	335..6799
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNTSMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	XM_015293005.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293005.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GATGAATGAT GCTATGGGAT TTGAATTGCC ATGGGTGTAT 
TTTGTCAGTC TCGTCATCTT TGGGTCATTT TTCGTACTAA ATCTTGTTCT TGGTGTATTG 
AGCGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACACAAGC 
ATGCCCACCA GCGAAACCGA GTCAGTGAAC ACAGAGAATG TGAGTGGAGA AGGAGAGAAC 
CCAGCATGCT GTGGGAGCTT ATGTCAAACC ATCTCAAAAT CCAAGTTCAG TCGACGCTGG 
CGTCGCTGGA ATCGATTTAA TCGAAGAAAA TGTAGAGCTG CAGTAAAATC TGTCACGTTT 
TATTGGCTTG TTATTGTCTT GGTCTTTCTG AACACATTAA CTATCTCATC GGAACATTAT 
AACCAACCTG ACTGGCTGAC CCAGATCCAA GATATCGCAA ATAAAGTTCT TCTGGCTTTG 
TTCACCTGTG AAATGTTAGT AAAGATGTAC AGCTTGGGTC TACAGGCTTA TTTTGTTTCT 
CTTTTTAACC GCTTTGATTG CTTCGTTGTT TGTGGTGGCA TTGTTGAAAC CATTTTGGTG 
GAGTTGGAAA TTATGTCACC CCTTGGAATT TCAGTGTTTC GCTGTGTTCG TCTCCTGCGT 
ATTTTTAAAG TAACAAGGCA CTGGGCATCC CTGAGCAACT TGGTAGCATC CTTGTTAAAC 
TCAATGAAGT CCATTGCTTC ACTGTTGCTT CTGCTTTTCC TCTTCATCAT AATCTTCTCG 
TTGCTTGGAA TGCAGCTGTT TGGAGGCAAA TTTAATTTTG ATGAAACTCA AACAAAACGG 
AGCACCTTCG ATAATTTTCC ACAAGCTCTT TTAACTGTAT TCCAGATTCT AACAGGTGAA 
GACTGGAATG CTGTTATGTA TGATGGCATA ATGGCGTATG GTGGCCCATC ATCGTCAGGC 
ATGATAGTCT GCATATATTT CATTATCCTC TTCATCTGCG GTAACTATAT CTTGCTAAAT 
GTCTTCTTGG CCATTGCTGT GGATAACTTG GCAGATGCTG AAAGTCTAAA TACAGCTCAG 
AAAGAAGAAG CAGAAGAAAA GGAAAGGAAA AAGAATGCAA GAAAAGAAAG TCTGGAAAAT 
AAAAAAAGTG AGAAGTCAGA AGGTGACCAA AAGAAGCCCA AGGATAGTAA GGTGACAATT 
GCTGAATATG GAGAAGGAGA GGATGAAGAT AAAGATCCCT ATCCACCCTG TGATGTACCA 
GTAGGTGAAG ATGAAGAAGA TGAGGAGGAT GAACCTGAGG TACCAGCAGG CCCACGTCCC 
CGGAGAATAT CAGAACTAAA CATGAAGGAG AAGATAACAC CGATCCCTGA AGGGAGTGCC 
TTCTTCATTT TCAGCAGCAC CAATCCAATT CGAGTGGGAT GCCACAGGCT CATCAATCAC 
CACATCTTTA CCAATCTCAT CCTTGTCTTC ATCATGCTGA GCAGTGTTTC CCTAGCAGCA 
GAAGATCCGA TTCGCAGCCA CTCTTTTCGA AATAATATCC TAGGGTATGC AGATTATGTC 
TTCACTAGTA TGTTTACATT TGAGATCATT TTGAAGATGA CAGCTTTCGG AGCATTCCTT 
CATAAAGGGT CCTTCTGCAG AAATTACTTT AATTTGCTGG ATTTGCTGGT TGTGGGTGTT 
TCTCTGGTAT CATTTGGTAT TCAGTCAAGT GCTATCTCAG TTGTGAAGAT CCTCAGAGTT 
TTGAGAGTCT TGAGGCCCCT TAGGGCTATA AACAGAGCAA AAGGACTTAA GCACGTTGTT 
CAGTGCGTCT TTGTGGCTAT TAGAACTATT GGTAATATCA TGATTGTTAC AACTCTGCTG 
CAGTTCATGT TTGCTTGTAT TGGAGTGCAG CTCTTCAAGG GAAAATTCTA CAAGTGCACT 
GATGAGGCAA AACAGAATCC AGAGGAATGC CGGGGGATAT ACATTGTTTA TAAAGATGGA 
GATGTTGATA ATCCAATGGT CAAAGAGAGG GTCTGGCAAA ATAGTGATTT CAACTTCGAC 
AATGTTCTGT CTGCTATGAT GGCCCTTTTC ACAGTGTCTA CTTTTGAAGG CTGGCCAGCG 
CTGCTGTACA AAGCCATTGA TTCCAATGGC GAGAATGTAG GACCTGTGTA CAACTACAGA 
GTGGAGATCT CCATTTTTTT CATCATCTAC ATCATCATTA TTGCTTTCTT TATGATGAAC 
ATATTTGTTG GTTTTGTCAT TGTTACCTTT CAAGAACAGG GAGAACAAGA ATACAAGAAC 
TGTGAGCTGG ACAAAAATCA GCGTCAGTGC GTGGAATATG CCTTGAAGGC TCGTCCTCTT 
CGTAGATACA TTCCAAAAAA TCCTTACCAG TATAAGTTCT GGTATGTGGT GAATTCTACT 
GGATTTGAAT ACATCATGTT TGTCCTCATC ATGCTGAACA CACTTTGCCT GGCTATGCAG 
CACTATGGAC AGTCTAAGCT CTTTAATGAT GCGATGGACA TTATGAATAT GGTTTTCACG 
GGAGTGTTCA CTGTTGAAAT GGTTTTGAAG CTGATTGCAT TCAAACCCAA GCACTATTTC 
ACTGATGCAT GGAACACTTT TGATGCCTTA ATTGTTGTTG GTAGCGTCGT TGATATTGCT 
ATAACAGAAG TTAATCCAAA GCCAACTGAA ACAGTCACAA CTGATGAGTC TGGGAACTCC 
GAAGACAGCG CAAGAATCTC CATCACTTTT TTCCGTCTTT TCCGAGTGAT GAGGTTGGTG 
AAGCTGCTTA GCAGAGGAGA AGGAATCAGA ACTTTATTGT GGACATTTAT TAAGTCATTT 
CAGGCTCTGC CTTATGTTGC CCTTCTGATA GCTATGCTGT TCTTCATCTA TGCTGTCATT 
GGAATGCAGG TATTTGGAAA AGTGGCCATG AGAGACAACA ATCAAATAAA CAGAAACAAC 
AATTTTCAAA CTTTCCCCCA AGCTGTGCTG CTTCTCTTCA GGTGTGCAAC TGGAGAAGCC 
TGGCAGGAAA TCATGCTGGC ATGTTTGCCT GGAAAACGCT GTGATCCAGA ATCTGATTAT 
AATCCAGGGG AAGAATACAC ATGTGGAAGC AATTTTGCCA TCATTTATTT TATCAGTTTT 
TATATGCTTT GTGCATTTTT GATAATAAAC TTATTTGTGG CTGTCATTAT GGATAACTTT 
GACTATTTGA CACGTGACTG GTCCATTCTG GGTCCTCATC ATTTGGATGA GTTCAAAAGG 
ATATGGTCAG AATATGACCC TGAAGCAAAG GGAAGGATAA AGCATCTTGA TGTGGTTACG 
TTACTGAGAC GCATTCAGCC ACCACTTGGT TTTGGCAAAT TATGCCCTCA CCGGGTGGCC 
TGCAAGAGGT TAGTGGCCAT GAATATGCCA CTAAATAGTG ACGGAACCGT CATGTTCAAT 
GCTACTTTGT TTGCTTTGGT TAGGACTGCT CTAAAGATAA AAACAGAAGG TAACCTAGAG 
CAAGCAAATG AAGAACTTAG AGCAGTTATA AAGAAAATAT GGAAGAAAAC TAGCATGAAA 
TTACTTGACC AAGTTGTCCC TCCGGCTGGC GATGATGAGG TAACCGTGGG GAAGTTCTAT 
GCCACCTTCC TGATACAGGA CTACTTTAGG AAATTCAAGA AACGCAAAGA ACAAGGACTG 
GTGGGGAAAT ATCCTGCGAA GAATACCACG ATTGCTCTGC AGGCAGGACT AAGGACACTT 
CATGATATTG GCCCTGAAAT ACGCCGAGCT ATATCCTGTG ACTTGCAAGA TGATGAACCA 
GAGGAAAACA ATCCAGACGA GGAGGAGGAA GTTTATAAAA GGAATGGTGC CCTCTTTGGC 
AACCATATAA ACCATATTAG CAGTGACAGA CGAGATTCAT TTCAGCAGAT CAACACCACG 
CACCGTCCCT TACATGTGCA GCGGCCTTCA ATTCCATCTG CAAGTGATAC TGAGAAAAAT 
ATATATCCTC ATACAGGAAA TTCTGTGTAC CACAATCATC ATAACCACAA CTCAGTAGGA 
AAACAAGTTC CAAACTCAAC AAATGCCAAT CTCAATAATG CCAACGTATC CAAAGTTGTT 
CACGGAAAAC ACGCTAATTT TGGAAGTCAC GAGCATAGAT CTGAAAATGG TTACCATTCG 
TACTCTAGAG CTGACCATGA GAAGCGTAGA AGGCCAAGCA GTAGGAGAAC CCGCTACTAC 
GAAACATATA TTAGGTCTGA CTCTGGTGAT GGCCGTCGAC CAACTATTTG CCGTGAAGAA 
CGTGACATTC GAGACTATTG CAATGATGAT CATTATTTGG GAGAGCAGGA ATATTATAGT 
GGAGAAGAAT ACTATGAGGA AGACAGCATG TTGTCAGGAA ACAGACATGT GTATGATTAC 
CACTGTAGAC ATCACTGCCA TGACTCAGAT TTTGAGAGAC CAAAAGGTTA CCATCACCCA 
CATGGGTTTT TTGAGGAAGA TGATTCACAG ACCTGTTATG ATACGAAAAG ATCTCCTAGG 
AGACGGCTGC TACCTCCAAC ACCAGCATCA AATAGACGAT CTTCCTTTAA TTTTGAATGC 
CTCCGCCGAC AGAGTAGTCA AGATGATATA CCACTCTCTC CAAATTTCCA TCATCGCACT 
GCTTTACCAC TTCACCTAAT GCAACAGCAG GTCATGGCTG TTGCAGGTCT GGATTCCAGT 
AAAGCTCATA AACATTCACC AAGTCGTTCC ACGCGTTCAT GGGCTACCCC ACCTGCAACC 
CCTCCCAACA GGGACCATAC CCCATACTAT ACACCTCTAA TCCAAGTTGA CAGGGCTGAA 
TCTACAGAAC ATATGAATGG TAGCCTGCCT TCCTTGCACA GGAGCTCCTG GTACACAGAC 
GACCCTGATA TCTCCTACCG AACATTTACA CCAGCCAATT TAACAGTACC TAATGACTTT 
AGGCATAAAC ACAGTGACAA ACAGAGGAGT GCAGACAGTT TGGTAGAAGC GGTACTTATA 
TCTGAAGGCC TAGGAAGGTA TGCAAAGGAC CCTAAATTTG TTTCAGCTAC AAAACATGAG 
ATTGCTGATG CTTGTGACAT GACTATCGAT GAGATGGAAA GTGCAGCAAG TAATCTTCTC 
AATGGAAATA TTAGCAATGG GACCAATGGG GATATGTTTC CCATTTTAAG CAGACAAGAT 
TATGAACTTC AAGATTTTGG TCCTGGCTAC AGTGATGAAG AACCAGAACC AGGACGATAT 
GAAGAAGACT TAGCTGATGA AATGATATGC ATTACAAGCT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_205034.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990365.1 Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), mRNA.

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CloneID	OGa28272
Clone ID Related Accession (Same CDS sequence)	NM_205034.2
Accession Version	NM_205034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Gallus gallus(chicken)
Product	voltage-dependent L-type calcium channel subunit alpha-1D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF027602.1, AC171014.3, AADN05000356.1 and CO421703.1. On Aug 24, 2012 this sequence version replaced NM_205034.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: AF027602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03354471, SAMN03354473 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCCAAAGC CAACTGAAAC AGTCACAACT GATGAGTCTG GGAACTCCGA AGACAGCGCA 
AGAATCTCCA TCACTTTTTT CCGTCTTTTC CGAGTGATGA GGTTGGTGAA GCTGCTTAGC 
AGAGGAGAAG GAATCAGAAC TTTATTGTGG ACATTTATTA AGTCATTTCA GGCTCTGCCT 
TATGTTGCCC TTCTGATAGC TATGCTGTTC TTCATCTATG CTGTCATTGG AATGCAGGTA 
TTTGGAAAAG TGGCCATGAG AGACAACAAT CAAATAAACA GAAACAACAA TTTTCAAACT 
TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG TGTGCAACTG GAGAAGCCTG GCAGGAAATC 
ATGCTGGCAT GTTTGCCTGG AAAACGCTGT GATCCAGAAT CTGATTATAA TCCAGGGGAA 
GAATACACAT GTGGAAGCAA TTTTGCCATC ATTTATTTTA TCAGTTTTTA TATGCTTTGT 
GCATTTTTGA TAATAAACTT ATTTGTGGCT GTCATTATGG ATAACTTTGA CTATTTGACA 
CGTGACTGGT CCATTCTGGG TCCTCATCAT TTGGATGAGT TCAAAAGGAT ATGGTCAGAA 
TATGACCCTG AAGCAAAGGG AAGGATAAAG CATCTTGATG TGGTTACGTT ACTGAGACGC 
ATTCAGCCAC CACTTGGTTT TGGCAAATTA TGCCCTCACC GGGTGGCCTG CAAGAGGTTA 
GTGGCCATGA ATATGCCACT AAATAGTGAC GGAACCGTCA TGTTCAATGC TACTTTGTTT 
GCTTTGGTTA GGACTGCTCT AAAGATAAAA ACAGAAGGTA ACCTAGAGCA AGCAAATGAA 
GAACTTAGAG CAGTTATAAA GAAAATATGG AAGAAAACTA GCATGAAATT ACTTGACCAA 
GTTGTCCCTC CGGCTGGCGA TGATGAGGTA ACCGTGGGGA AGTTCTATGC CACCTTCCTG 
ATACAGGACT ACTTTAGGAA ATTCAAGAAA CGCAAAGAAC AAGGACTGGT GGGGAAATAT 
CCTGCGAAGA ATACCACGAT TGCTCTGCAG GCAGGACTAA GGACACTTCA TGATATTGGC 
CCTGAAATAC GCCGAGCTAT ATCCTGTGAC TTGCAAGATG ATGAACCAGA GGAAAACAAT 
CCAGACGAGG AGGAGGAAGT TTATAAAAGG AATGGTGCCC TCTTTGGCAA CCATATAAAC 
CATATTAGCA GTGACAGACG AGATTCATTT CAGCAGATCA ACACCACGCA CCGTCCCTTA 
CATGTGCAGC GGCCTTCAAT TCCATCTGCA AGTGATACTG AGAAAAATAT ATATCCTCAT 
ACAGGAAATT CTGTGTACCA CAATCATCAT AACCACAACT CAGTAGGAAA ACAAGTTCCA 
AACTCAACAA ATGCCAATCT CAATAATGCC AACGTATCCA AAGTTGTTCA CGGAAAACAC 
GCTAATTTTG GAAGTCACGA GCATAGATCT GAAAATGGTT ACCATTCGTA CTCTAGAGCT 
GACCATGAGA AGCGTAGAAG GCCAAGCAGT AGGAGAACCC GCTACTACGA AACATATATT 
AGGTCTGACT CTGGTGATGG CCGTCGACCA ACTATTTGCC GTGAAGAACG TGACATTCGA 
GACTATTGCA ATGATGATCA TTATTTGGGA GAGCAGGAAT ATTATAGTGG AGAAGAATAC 
TATGAGGAAG ACAGCATGTT GTCAGGAAAC AGACATGTGT ATGATTACCA CTGTAGACAT 
CACTGCCATG ACTCAGATTT TGAGAGACCA AAAGGTTACC ATCACCCACA TGGGTTTTTT 
GAGGAAGATG ATTCACAGAC CTGTTATGAT ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA 
CCTCCAACAC CAGCATCAAA TAGACGATCT TCCTTTAATT TTGAATGCCT CCGCCGACAG 
AGTAGTCAAG ATGATATACC ACTCTCTCCA AATTTCCATC ATCGCACTGC TTTACCACTT 
CACCTAATGC AACAGCAGGT CATGGCTGTT GCAGGTCTGG ATTCCAGTAA AGCTCATAAA 
CATTCACCAA GTCGTTCCAC GCGTTCATGG GCTACCCCAC CTGCAACCCC TCCCAACAGG 
GACCATACCC CATACTATAC ACCTCTAATC CAAGTTGACA GGGCTGAATC TACAGAACAT 
ATGAATGGTA GCCTGCCTTC CTTGCACAGG AGCTCCTGGT ACACAGACGA CCCTGATATC 
TCCTACCGAA CATTTACACC AGCCAATTTA ACAGTACCTA ATGACTTTAG GCATAAACAC 
AGTGACAAAC AGAGGAGTGC AGACAGTTTG GTAGAAGCGG TACTTATATC TGAAGGCCTA 
GGAAGGTATG CAAAGGACCC TAAATTTGTT TCAGCTACAA AACATGAGAT TGCTGATGCT 
TGTGACATGA CTATCGATGA GATGGAAAGT GCAGCAAGTA ATCTTCTCAA TGGAAATATT 
AGCAATGGGA CCAATGGGGA TATGTTTCCC ATTTTAAGCA GACAAGATTA TGAACTTCAA 
GATTTTGGTC CTGGCTACAG TGATGAAGAA CCAGAACCAG GACGATATGA AGAAGACTTA 
GCTGATGAAA TGATATGCAT TACAAGCTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990365.1
CDS	253..6825
Translation	MQHHQQQQPEQHPEEANYASSTRIPLPGDGPTTQSNSSAPSKQTVLSWQAAIDAARQAKAAQNMNTTTAQPVGSLSQRKRQQYAKSKKQGNTSNSRPPRALFCLSLNNPIRRACISLVEWKPFDIFILLSIFANCVALAVYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVVVGLFSVILEQLTKETEGGSHSGGKPGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKSCFLIDSDILVEEDPAPCAFSGNGRQCVMNGTECKGGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGCEWPWIYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENDEEADEEGKRNRVTLADLMEEKKKSRLSCFGRSSNKHASMPTSETESVNTENVSGEGENPACCGSLCQTISKSKFSRRWRRWNRFNRRKCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGIVETILVELEIMSPLGISVFRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKNARKESLENKKSEKSEGDQKKPKDSKVTIAEYGEGEDEDKDPYPPCDVPVGEDEEDEEDEPEVPAGPRPRRISELNMKEKITPIPEGSAFFIFSSTNPIRVGCHRLINHHIFTNLILVFIMLSSVSLAAEDPIRSHSFRNNILGYADYVFTSMFTFEIILKMTAFGAFLHKGSFCRNYFNLLDLLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYKCTDEAKQNPEECRGIYIVYKDGDVDNPMVKERVWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENVGPVYNYRVEISIFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSTGFEYIMFVLIMLNTLCLAMQHYGQSKLFNDAMDIMNMVFTGVFTVEMVLKLIAFKPKIFVRKKERWLGYFSDAWNTFDSLIVIGSIVDVVLSEADPKPTETVTTDESGNSEDSARISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQVFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKRCDPESDYNPGEEYTCGSNFAIIYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEENNPDEEEEVYKRNGALFGNHINHISSDRRDSFQQINTTHRPLHVQRPSIPSASDTEKNIYPHTGNSVYHNHHNHNSVGKQVPNSTNANLNNANVSKVVHGKHANFGSHEHRSENGYHSYSRADHEKRRRPSSRRTRYYETYIRSDSGDGRRPTICREERDIRDYCNDDHYLGEQEYYSGEEYYEEDSMLSGNRHVYDYHCRHHCHDSDFERPKGYHHPHGFFEEDDSQTCYDTKRSPRRRLLPPTPASNRRSSFNFECLRRQSSQDDIPLSPNFHHRTALPLHLMQQQVMAVAGLDSSKAHKHSPSRSTRSWATPPATPPNRDHTPYYTPLIQVDRAESTEHMNGSLPSLHRSSWYTDDPDISYRTFTPANLTVPNDFRHKHSDKQRSADSLVEAVLISEGLGRYAKDPKFVSATKHEIADACDMTIDEMESAASNLLNGNISNGTNGDMFPILSRQDYELQDFGPGYSDEEPEPGRYEEDLADEMICITSL

Target ORF information:

RefSeq Version	NM_205034.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus calcium voltage-gated channel subunit alpha1 D (CACNA1D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205034.2

ORF Insert Sequence:

ATGCAGCACC ACCAGCAGCA GCAGCCCGAG CAGCACCCAG AAGAGGCGAA CTATGCAAGC 
AGCACCAGAA TTCCTCTCCC TGGTGACGGA CCAACTACTC AGTCCAACAG TTCAGCACCA 
TCCAAGCAAA CTGTTCTGTC TTGGCAAGCT GCAATTGATG CTGCTAGACA AGCAAAGGCT 
GCCCAAAATA TGAATACCAC CACAGCCCAG CCTGTAGGAT CTCTGTCCCA AAGAAAACGC 
CAGCAATATG CCAAGAGCAA AAAACAGGGT AATACGTCTA ATAGTCGGCC ACCCCGTGCC 
CTTTTCTGTT TATCCCTCAA CAACCCAATA CGAAGAGCCT GCATTAGTCT AGTAGAATGG 
AAACCATTTG ACATATTTAT ACTACTGTCT ATTTTTGCCA ATTGTGTGGC CTTAGCTGTT 
TACATCCCAT TCCCAGAAGA TGATTCTAAT TCAACTAATC ATAACTTGGA AAAAGTAGAA 
TATGCCTTCC TGATAATTTT TACAGTTGAA ACATTTTTGA AGATTATAGC ATATGGATTA 
TTATTACACC CCAATGCGTA TGTTAGAAAT GGATGGAATT TATTGGATTT TGTAATAGTA 
GTAGTAGGAT TATTTAGTGT AATATTGGAA CAATTAACCA AAGAAACAGA AGGTGGCAGC 
CACTCAGGTG GCAAACCTGG AGGCTTTGAT GTCAAAGCCC TAAGAGCCTT TCGTGTATTG 
CGACCTCTCC GGCTTGTATC AGGAGTGCCC AGTTTACAAG TTGTCCTGAA CTCCATTATA 
AAAGCCATGG TTCCCCTGCT CCATATTGCC CTTTTGGTAT TGTTTGTAAT CATAATCTAT 
GCTATTATAG GATTGGAACT TTTTATTGGA AAAATGCACA AATCTTGTTT TCTTATTGAT 
TCAGATATAC TAGTTGAAGA AGATCCAGCA CCTTGTGCAT TCTCAGGGAA TGGACGTCAG 
TGTGTCATGA ATGGCACTGA ATGTAAGGGT GGTTGGGTTG GACCGAATGG AGGCATAACG 
AACTTCGATA ATTTTGCATT TGCAATGCTG ACTGTATTCC AGTGTATAAC CATGGAAGGA 
TGGACTGATG TGTTATACTG GGTAAATGAT GCAATAGGAT GTGAATGGCC ATGGATCTAT 
TTTGTTAGTC TTATCATCCT TGGCTCATTT TTCGTACTTA ACCTGGTTCT TGGTGTACTT 
AGTGGGGAAT TTTCCAAGGA AAGAGAAAAA GCCAAGGCCC GAGGGGACTT TCAGAAGCTA 
CGTGAAAAAC AACAGCTTGA AGAAGATCTG AAGGGATATT TAGACTGGAT TACACAGGCA 
GAAGACATTG ATCCTGAGAA TGATGAAGAG GCTGATGAAG AAGGGAAACG GAACAGGGTT 
ACACTGGCTG ACTTAATGGA AGAAAAGAAG AAAAGCAGAC TTAGTTGCTT TGGCCGGTCT 
TCCAATAAAC ATGCAAGCAT GCCCACCAGC GAAACCGAGT CAGTGAACAC AGAGAATGTG 
AGTGGAGAAG GAGAGAACCC AGCATGCTGT GGGAGCTTAT GTCAAACCAT CTCAAAATCC 
AAGTTCAGTC GACGCTGGCG TCGCTGGAAT CGATTTAATC GAAGAAAATG TAGAGCTGCA 
GTAAAATCTG TCACGTTTTA TTGGCTTGTT ATTGTCTTGG TCTTTCTGAA CACATTAACT 
ATCTCATCGG AACATTATAA CCAACCTGAC TGGCTGACCC AGATCCAAGA TATCGCAAAT 
AAAGTTCTTC TGGCTTTGTT CACCTGTGAA ATGTTAGTAA AGATGTACAG CTTGGGTCTA 
CAGGCTTATT TTGTTTCTCT TTTTAACCGC TTTGATTGCT TCGTTGTTTG TGGTGGCATT 
GTTGAAACCA TTTTGGTGGA GTTGGAAATT ATGTCACCCC TTGGAATTTC AGTGTTTCGC 
TGTGTTCGTC TCCTGCGTAT TTTTAAAGTA ACAAGGCACT GGGCATCCCT GAGCAACTTG 
GTAGCATCCT TGTTAAACTC AATGAAGTCC ATTGCTTCAC TGTTGCTTCT GCTTTTCCTC 
TTCATCATAA TCTTCTCGTT GCTTGGAATG CAGCTGTTTG GAGGCAAATT TAATTTTGAT 
GAAACTCAAA CAAAACGGAG CACCTTCGAT AATTTTCCAC AAGCTCTTTT AACTGTATTC 
CAGATTCTAA CAGGTGAAGA CTGGAATGCT GTTATGTATG ATGGCATAAT GGCGTATGGT 
GGCCCATCAT CGTCAGGCAT GATAGTCTGC ATATATTTCA TTATCCTCTT CATCTGCGGT 
AACTATATCT TGCTAAATGT CTTCTTGGCC ATTGCTGTGG ATAACTTGGC AGATGCTGAA 
AGTCTAAATA CAGCTCAGAA AGAAGAAGCA GAAGAAAAGG AAAGGAAAAA GAATGCAAGA 
AAAGAAAGTC TGGAAAATAA AAAAAGTGAG AAGTCAGAAG GTGACCAAAA GAAGCCCAAG 
GATAGTAAGG TGACAATTGC TGAATATGGA GAAGGAGAGG ATGAAGATAA AGATCCCTAT 
CCACCCTGTG ATGTACCAGT AGGTGAAGAT GAAGAAGATG AGGAGGATGA ACCTGAGGTA 
CCAGCAGGCC CACGTCCCCG GAGAATATCA GAACTAAACA TGAAGGAGAA GATAACACCG 
ATCCCTGAAG GGAGTGCCTT CTTCATTTTC AGCAGCACCA ATCCAATTCG AGTGGGATGC 
CACAGGCTCA TCAATCACCA CATCTTTACC AATCTCATCC TTGTCTTCAT CATGCTGAGC 
AGTGTTTCCC TAGCAGCAGA AGATCCGATT CGCAGCCACT CTTTTCGAAA TAATATCCTA 
GGGTATGCAG ATTATGTCTT CACTAGTATG TTTACATTTG AGATCATTTT GAAGATGACA 
GCTTTCGGAG CATTCCTTCA TAAAGGGTCC TTCTGCAGAA ATTACTTTAA TTTGCTGGAT 
TTGCTGGTTG TGGGTGTTTC TCTGGTATCA TTTGGTATTC AGTCAAGTGC TATCTCAGTT 
GTGAAGATCC TCAGAGTTTT GAGAGTCTTG AGGCCCCTTA GGGCTATAAA CAGAGCAAAA 
GGACTTAAGC ACGTTGTTCA GTGCGTCTTT GTGGCTATTA GAACTATTGG TAATATCATG 
ATTGTTACAA CTCTGCTGCA GTTCATGTTT GCTTGTATTG GAGTGCAGCT CTTCAAGGGA 
AAATTCTACA AGTGCACTGA TGAGGCAAAA CAGAATCCAG AGGAATGCCG GGGGATATAC 
ATTGTTTATA AAGATGGAGA TGTTGATAAT CCAATGGTCA AAGAGAGGGT CTGGCAAAAT 
AGTGATTTCA ACTTCGACAA TGTTCTGTCT GCTATGATGG CCCTTTTCAC AGTGTCTACT 
TTTGAAGGCT GGCCAGCGCT GCTGTACAAA GCCATTGATT CCAATGGCGA GAATGTAGGA 
CCTGTGTACA ACTACAGAGT GGAGATCTCC ATTTTTTTCA TCATCTACAT CATCATTATT 
GCTTTCTTTA TGATGAACAT ATTTGTTGGT TTTGTCATTG TTACCTTTCA AGAACAGGGA 
GAACAAGAAT ACAAGAACTG TGAGCTGGAC AAAAATCAGC GTCAGTGCGT GGAATATGCC 
TTGAAGGCTC GTCCTCTTCG TAGATACATT CCAAAAAATC CTTACCAGTA TAAGTTCTGG 
TATGTGGTGA ATTCTACTGG ATTTGAATAC ATCATGTTTG TCCTCATCAT GCTGAACACA 
CTTTGCCTGG CTATGCAGCA CTATGGACAG TCTAAGCTCT TTAATGATGC GATGGACATT 
ATGAATATGG TTTTCACGGG AGTGTTCACT GTTGAAATGG TTTTGAAGCT GATTGCATTC 
AAACCCAAGA TTTTTGTAAG AAAAAAGGAA AGATGGCTAG GCTATTTTAG TGATGCCTGG 
AATACGTTTG ACTCCCTCAT CGTTATTGGC AGCATAGTAG ACGTCGTTCT CAGTGAAGCT 
GATCCAAAGC CAACTGAAAC AGTCACAACT GATGAGTCTG GGAACTCCGA AGACAGCGCA 
AGAATCTCCA TCACTTTTTT CCGTCTTTTC CGAGTGATGA GGTTGGTGAA GCTGCTTAGC 
AGAGGAGAAG GAATCAGAAC TTTATTGTGG ACATTTATTA AGTCATTTCA GGCTCTGCCT 
TATGTTGCCC TTCTGATAGC TATGCTGTTC TTCATCTATG CTGTCATTGG AATGCAGGTA 
TTTGGAAAAG TGGCCATGAG AGACAACAAT CAAATAAACA GAAACAACAA TTTTCAAACT 
TTCCCCCAAG CTGTGCTGCT TCTCTTCAGG TGTGCAACTG GAGAAGCCTG GCAGGAAATC 
ATGCTGGCAT GTTTGCCTGG AAAACGCTGT GATCCAGAAT CTGATTATAA TCCAGGGGAA 
GAATACACAT GTGGAAGCAA TTTTGCCATC ATTTATTTTA TCAGTTTTTA TATGCTTTGT 
GCATTTTTGA TAATAAACTT ATTTGTGGCT GTCATTATGG ATAACTTTGA CTATTTGACA 
CGTGACTGGT CCATTCTGGG TCCTCATCAT TTGGATGAGT TCAAAAGGAT ATGGTCAGAA 
TATGACCCTG AAGCAAAGGG AAGGATAAAG CATCTTGATG TGGTTACGTT ACTGAGACGC 
ATTCAGCCAC CACTTGGTTT TGGCAAATTA TGCCCTCACC GGGTGGCCTG CAAGAGGTTA 
GTGGCCATGA ATATGCCACT AAATAGTGAC GGAACCGTCA TGTTCAATGC TACTTTGTTT 
GCTTTGGTTA GGACTGCTCT AAAGATAAAA ACAGAAGGTA ACCTAGAGCA AGCAAATGAA 
GAACTTAGAG CAGTTATAAA GAAAATATGG AAGAAAACTA GCATGAAATT ACTTGACCAA 
GTTGTCCCTC CGGCTGGCGA TGATGAGGTA ACCGTGGGGA AGTTCTATGC CACCTTCCTG 
ATACAGGACT ACTTTAGGAA ATTCAAGAAA CGCAAAGAAC AAGGACTGGT GGGGAAATAT 
CCTGCGAAGA ATACCACGAT TGCTCTGCAG GCAGGACTAA GGACACTTCA TGATATTGGC 
CCTGAAATAC GCCGAGCTAT ATCCTGTGAC TTGCAAGATG ATGAACCAGA GGAAAACAAT 
CCAGACGAGG AGGAGGAAGT TTATAAAAGG AATGGTGCCC TCTTTGGCAA CCATATAAAC 
CATATTAGCA GTGACAGACG AGATTCATTT CAGCAGATCA ACACCACGCA CCGTCCCTTA 
CATGTGCAGC GGCCTTCAAT TCCATCTGCA AGTGATACTG AGAAAAATAT ATATCCTCAT 
ACAGGAAATT CTGTGTACCA CAATCATCAT AACCACAACT CAGTAGGAAA ACAAGTTCCA 
AACTCAACAA ATGCCAATCT CAATAATGCC AACGTATCCA AAGTTGTTCA CGGAAAACAC 
GCTAATTTTG GAAGTCACGA GCATAGATCT GAAAATGGTT ACCATTCGTA CTCTAGAGCT 
GACCATGAGA AGCGTAGAAG GCCAAGCAGT AGGAGAACCC GCTACTACGA AACATATATT 
AGGTCTGACT CTGGTGATGG CCGTCGACCA ACTATTTGCC GTGAAGAACG TGACATTCGA 
GACTATTGCA ATGATGATCA TTATTTGGGA GAGCAGGAAT ATTATAGTGG AGAAGAATAC 
TATGAGGAAG ACAGCATGTT GTCAGGAAAC AGACATGTGT ATGATTACCA CTGTAGACAT 
CACTGCCATG ACTCAGATTT TGAGAGACCA AAAGGTTACC ATCACCCACA TGGGTTTTTT 
GAGGAAGATG ATTCACAGAC CTGTTATGAT ACGAAAAGAT CTCCTAGGAG ACGGCTGCTA 
CCTCCAACAC CAGCATCAAA TAGACGATCT TCCTTTAATT TTGAATGCCT CCGCCGACAG 
AGTAGTCAAG ATGATATACC ACTCTCTCCA AATTTCCATC ATCGCACTGC TTTACCACTT 
CACCTAATGC AACAGCAGGT CATGGCTGTT GCAGGTCTGG ATTCCAGTAA AGCTCATAAA 
CATTCACCAA GTCGTTCCAC GCGTTCATGG GCTACCCCAC CTGCAACCCC TCCCAACAGG 
GACCATACCC CATACTATAC ACCTCTAATC CAAGTTGACA GGGCTGAATC TACAGAACAT 
ATGAATGGTA GCCTGCCTTC CTTGCACAGG AGCTCCTGGT ACACAGACGA CCCTGATATC 
TCCTACCGAA CATTTACACC AGCCAATTTA ACAGTACCTA ATGACTTTAG GCATAAACAC 
AGTGACAAAC AGAGGAGTGC AGACAGTTTG GTAGAAGCGG TACTTATATC TGAAGGCCTA 
GGAAGGTATG CAAAGGACCC TAAATTTGTT TCAGCTACAA AACATGAGAT TGCTGATGCT 
TGTGACATGA CTATCGATGA GATGGAAAGT GCAGCAAGTA ATCTTCTCAA TGGAAATATT 
AGCAATGGGA CCAATGGGGA TATGTTTCCC ATTTTAAGCA GACAAGATTA TGAACTTCAA 
GATTTTGGTC CTGGCTACAG TGATGAAGAA CCAGAACCAG GACGATATGA AGAAGACTTA 
GCTGATGAAA TGATATGCAT TACAAGCTTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development and regeneration of hair cells share common functional features.
Proceedings of the National Academy of Sciences of the United States of America104(48)19108-13(2007 Nov)
Levic S,Nie L,Tuteja D,Harvey M,Sokolowski BH,Yamoah EN

Predominance of the alpha1D subunit in L-type voltage-gated Ca2+ channels of hair cells in the chicken's cochlea.
Proceedings of the National Academy of Sciences of the United States of America94(26)14883-8(1997 Dec)
Kollmar R,Montgomery LG,Fak J,Henry LJ,Hudspeth AJ

Hair cell-specific splicing of mRNA for the alpha1D subunit of voltage-gated Ca2+ channels in the chicken's cochlea.
Proceedings of the National Academy of Sciences of the United States of America94(26)14889-93(1997 Dec)
Kollmar R,Fak J,Montgomery LG,Hudspeth AJ

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CACNA1D cDNA ORF clone, Gallus gallus(chicken)

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