TJP2 cDNA ORF clone, Gallus gallus(chicken)

The following TJP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa48036 XM_025144668.1
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48036 XM_015280242.2
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48036 XM_015280246.2
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48036 XM_015280247.2
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48036 XM_015280245.1
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48036 XM_025144669.1
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48037 XM_015280243.2
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa48038 XM_015280244.2
Latest version!
Gallus gallus tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa00603 NM_204918.1
Latest version!
Gallus gallus tight junction protein 2 (TJP2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OGa48038 XM_015280244.1 Gallus gallus tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa48036 XM_015280247.1 Gallus gallus tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48036 XM_015280242.1 Gallus gallus tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OGa48037 XM_015280243.1 Gallus gallus tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa48036 XM_015280246.1 Gallus gallus tight junction protein 2 (TJP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280242.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135728.1
    CDS289..3780
    Translation

    Target ORF information:

    RefSeq Version XM_015280242.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280242.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135731.1
    CDS217..3708
    Translation

    Target ORF information:

    RefSeq Version XM_015280245.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280246.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135732.1
    CDS251..3742
    Translation

    Target ORF information:

    RefSeq Version XM_015280246.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280246.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280247.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135733.1
    CDS152..3643
    Translation

    Target ORF information:

    RefSeq Version XM_015280247.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280247.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135733.1
    CDS153..3644
    Translation

    Target ORF information:

    RefSeq Version XM_015280247.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280247.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280242.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135728.1
    CDS271..3762
    Translation

    Target ORF information:

    RefSeq Version XM_015280242.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280242.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_015280246.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135732.1
    CDS161..3652
    Translation

    Target ORF information:

    RefSeq Version XM_015280246.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_025144668.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000436.1
    CDS3131..6622
    Translation

    Target ORF information:

    RefSeq Version XM_025144668.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144668.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48036
    Clone ID Related Accession (Same CDS sequence) XM_015280242.2 , XM_015280245.1 , XM_015280246.1 , XM_015280247.2 , XM_015280247.1 , XM_015280242.1 , XM_015280246.2 , XM_025144668.1 , XM_025144669.1
    Accession Version XM_025144669.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000437.1
    CDS3079..6570
    Translation

    Target ORF information:

    RefSeq Version XM_025144669.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144669.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAGAAG CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGATGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GCAGGGAATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGACTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48037
    Clone ID Related Accession (Same CDS sequence) XM_015280243.2 , XM_015280243.1
    Accession Version XM_015280243.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAATGA AGACAGCGCA AGCTCTGCAG AGAATGTGGT CACATGCAGT CAAAAAGTTG 
    AGGATTTTAA GGGGGCACGC CCCAGGCATG GAAGAGCTGA TATGGGAACA GTACACTGTG
    ACCTTACAAA AGGATTCAAA ACGAGGATTT GGGATTGCAG TTTCTGGTGG CAGAGATAAC
    CCACATTTTG AAAATGGTGA AACCTCAATA GTCATTTCAG ATGTTCTCCC AGGTGGCCCA
    GCAGATGGAT TACTTCAAGA AAATGACCGC GTGGTGATTG TTAATGGGAC CCCAATGGAA
    AATGTTCCAC ATTCTTTTGC AGTACAACAG CTTAGGAAAA GTGGAAAAGT GGCCACCATT
    GTTGTGAAAA GACCACGGAA AGTGCAGGCT GCTGCTTTGA GGAGAAGCCC CTCCCTTGAC
    TATGAGGACA GAGCTTTAGA TGTAATGGAT GACCATGCAG AATTTGATGG CAAAAGTGCT
    CGAAGTGGCT ACAGTGAAAG AAGCTGGCGT AGTGGCAATG GAGGGCGCAG TCAAAGCTGG
    GGAAACAGCC TGGATCAGAG CTATAGAGAT GATCAAGACA GAGAGCACAA CCGAAGCAGA
    GGTCGTAGCA GGGAATGTAG CTACAGCCGT GATCAAAGTC GTGGTAGAAG CATTGATAGG
    AGCTTGGATC AAGACTATGG CAGAGATCGG AGCAGGGGAA GAAGCGTTGA CAGGGATGGT
    GGCTATGGAC AGGACTACAG AAGAGACTAC AGTCCAGCCA GTTACAGACA TGGATCTCAA
    CCTGATCCTA GATATGTGAG GGAGGTGAGA AGCCTAAGTC GAGACAGGCT TCATTCTCGT
    AGTCCTTCAC CTGAAACACG CCATCAGCAT GAGTACCTAG GACCACAGGA TCAGAATGGA
    CCCATCAGTG TTCTCTTAAC AAAAGGCAAA CATAATGAAG AATATGGTCT CCGACTTGGA
    AGTCAGATCT TCATAAAAGA AATGACCAGT ACTGGCCTAG CAACCAAAGA TGGCAACCTT
    CATGAAGGGG ATATCATTCT TAAGATCAAT GGTACAGTGA CAGAGAACAT GTCTTTAGCT
    GATGCCCGAA AATTGATTGA GAAGTCTCGG GGAAAGCTCC AGCTGGTTGT CCTCAGGGAC
    AGAAAGCAGA CCCTGCTCAA CATTCCTTCA TTGAATGACA GTGACTCAGA AATGGATGAT
    ATTTCTGAAA TAGAGTCAAA CAGATCGTTC TCCCCTCAAG ATGATAGATT GCATCATTCT
    GATCTGGATT TGCATTCATC CAATGAAAAG CTGAAAGAGA AAACGAATGC AAAAGATGAT
    CCATCCAATA GGATGTCCAG GATGGGAGCA ATGCCTACAC CATTTAAATC AACTGGTGAT
    ATTGCTACAA CTGCTGTCAC TGTAGTAGAT GCAAACAAAG AACCAAAGTA CCAAGATGAT
    GTAGCAGTGC CCCAGCCAAA AGCAGTTACA AGAACGATTC TTAAACCCAG CCCAGAAGAT
    GAAGCAATAT ATGGTCCTAA TACAAAAATG GTAAGATTCA AGAAGGGGGA CAGTGTTGGT
    CTGCGACTTG CTGGTGGAAA TGATGTAGGG ATATTTATTG CTGGAATCCA AGAAGGCACC
    TCAGCTGACC AGGAAGGACT TCAGGAAGGA GATCAGATTC TCAAGGTAAA CGCTCAAGAT
    TTCAGAGGCA TTGTTCGGGA GGATGCTGTT TTATACCTAC TAGAAATTCC CAAAGGTGAT
    ACAGTGACAA TTTTGGCTCA AAGCAAATAT GAAGTATACA GAGACATCAT GGCCTGTGGC
    AGAGGAGATT CATTCTTCAT CAGAAGCCAC TTTGAGTGTG AAAAAGAGTC ACCACAGAGC
    TTAGCCTTCA CCAGAGGGGA GATCTTTAGA GTAGTTGATA CTCTGTATGA TGGCAAACTA
    GGAAACTGGC TGGCTGTGAG AATTGGAAAT GAACTTGAAA AGGGCCTCAT TCCAAACAGG
    AGCAGAGCTG AGCAGATGGC CAGTGTTCAG AATGCCCAGA AAGATGGCCC TAGTGACAGG
    GCAGACTTCT GGAGAACACG CGGCCAGCGA TCTGGAGTGA AGAAGAATTT GAGGAAGAGT
    CGTGAAGATC TGACAGCAAT TGTATCAGTG GGCACAAAAT TCCCAGCTTA TGAACGAGTT
    CAGTTGCGTG AAGCTGGTTT TAAGAGACCT GTTGTGATAT TTGGCCCCAT TGCAGATGTT
    GCTATGGAGA AGTTGTCAAC TGACTTGCCT CACCTGTACC AGACTGCAAA GACAGAACCC
    AGAGATGCAG GTTCAGAGAA GGCAACTGGG GTAGTACGCT TGAACACTGT GAGGCAAATC
    ATTGAGCAGG ATAAACATGC TCTCTTGGAT GTGACTCCTA AAGCAGTGGA TCTGCTGAAT
    TATACCCAGT GGTTTCCCAT TGTAGTCTTC TTTAACCCAG ACAGTAAGCA GGGCGTGAAA
    ACGATGAGAC AAAGGCTGTG TTCCACATCT AACAAAAGCT CGAGGAAGCT TTATGAGCAA
    GCAAACAAGC TGAAGAAGAC TTGTTCCCAC CTCTTTACAG CCACCATCAA TTTGAATTCA
    GCTAATGATA GCTGGTATGG TAGTCTGAAG GATACAATTC AGCAACAGCA AGGTGAAGCA
    GTTTGGGTGT CAGAAGGAAA GGTAGATGGC ATGGAAGATG ACGCAGATGA TCGCATGTCT
    TACCTTACTG CCATGGGTGC TGACTATTTG AGTTGTGACA GTCGGTTGAT CAGTGACTTA
    GAAGATACAG ATGGAGAGGG AGGTGCATAC ACTGATAATG AACTTGATGA ACCTTTGGAT
    GAACCAAGAA TCTCATCTGT TGGCCGGTCC TCTGAACCTG TACATCATGA AGAGAATATA
    AAGAAATTTA GCTTGGAACA AAGAACTCCA TTGAGGAAAG CTGGTAGCAG GGAGATCCTT
    AGAGAACCTA GTCCACCTCC AGCATTCAAG CCTGAACCAC CAAAGGGGAA GTTACAAAAC
    AAAGAGGATC TGTATGACTT CCCTAAGAAC TATGACTCCA AACCAAGTAG CATCCATGTC
    AGCAGTGAGA CTTCAACTGT ATCAGCCAAA GCAGCACCAC CACCTGTTTC TGTGAAACCT
    ACTTTTGGGC GTCCCATCCT GAGAAATACT CAGCCAGCAG TCCCAAGTGC AGAAGAAGAA
    GAGGAGGGAA AGTTGGAAGA GGAAGGGAGT GAACAAGAAA ATACTCCAAA ATCTGTGCTG
    AGGAAAGTAA AAATATTTGA GGAGATGGAT CATAAGGCAA GACTGCAAAG AATGCAAGAG
    CTACAGGAGG CCCAGAATGC CAGGATTGAA ATAGCCCAGA AGCATCCAGA CATTTATGCT
    GTCCCTGTCA AAACACAGAA GTCAGACCAG AACTGGCCCC AGCCCATCAG CTGCCGACCT
    CCAGAACCCC AGAAACCTCC TACCAGACCT TACCTGGAGG ATCGTGGCAG CTGTGGCAGT
    GATGGAGAGG AGGAGGAGGA GTACCGTCGG CAGCTATCAG ACCACTCTAA GAAAGGTTAC
    TACGGACAGC CACCCAGATA CAGAGACACA GAACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135729.1
    CDS10..3588
    Translation

    Target ORF information:

    RefSeq Version XM_015280243.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAATGA AGACAGCGCA AGCTCTGCAG AGAATGTGGT CACATGCAGT CAAAAAGTTG 
    AGGATTTTAA GGGGGCACGC CCCAGGCATG GAAGAGCTGA TATGGGAACA GTACACTGTG
    ACCTTACAAA AGGATTCAAA ACGAGGATTT GGGATTGCAG TTTCTGGTGG CAGAGATAAC
    CCACATTTTG AAAATGGTGA AACCTCAATA GTCATTTCAG ATGTTCTCCC AGGTGGCCCA
    GCAGATGGAT TACTTCAAGA AAATGACCGC GTGGTGATTG TTAATGGGAC CCCAATGGAA
    AATGTTCCAC ATTCTTTTGC AGTACAACAG CTTAGGAAAA GTGGAAAAGT GGCCACCATT
    GTTGTGAAAA GACCACGGAA AGTGCAGGCT GCTGCTTTGA GGAGAAGCCC CTCCCTTGAC
    TATGAGGACA GAGCTTTAGA TGTAATGGAT GACCATGCAG AATTTGATGG CAAAAGTGCT
    CGAAGTGGCT ACAGTGAAAG AAGCTGGCGT AGTGGCAATG GAGGGCGCAG TCAAAGCTGG
    GGAAACAGCC TGGATCAGAG CTATAGAGAT GATCAAGACA GAGAGCACAA CCGAAGCAGA
    GGTCGTAGCA GGGAATGTAG CTACAGCCGT GATCAAAGTC GTGGTAGAAG CATTGATAGG
    AGCTTGGATC AAGACTATGG CAGAGATCGG AGCAGGGGAA GAAGCGTTGA CAGGGATGGT
    GGCTATGGAC AGGACTACAG AAGAGACTAC AGTCCAGCCA GTTACAGACA TGGATCTCAA
    CCTGATCCTA GATATGTGAG GGAGGTGAGA AGCCTAAGTC GAGACAGGCT TCATTCTCGT
    AGTCCTTCAC CTGAAACACG CCATCAGCAT GAGTACCTAG GACCACAGGA TCAGAATGGA
    CCCATCAGTG TTCTCTTAAC AAAAGGCAAA CATAATGAAG AATATGGTCT CCGACTTGGA
    AGTCAGATCT TCATAAAAGA AATGACCAGT ACTGGCCTAG CAACCAAAGA TGGCAACCTT
    CATGAAGGGG ATATCATTCT TAAGATCAAT GGTACAGTGA CAGAGAACAT GTCTTTAGCT
    GATGCCCGAA AATTGATTGA GAAGTCTCGG GGAAAGCTCC AGCTGGTTGT CCTCAGGGAC
    AGAAAGCAGA CCCTGCTCAA CATTCCTTCA TTGAATGACA GTGACTCAGA AATGGATGAT
    ATTTCTGAAA TAGAGTCAAA CAGATCGTTC TCCCCTCAAG ATGATAGATT GCATCATTCT
    GATCTGGATT TGCATTCATC CAATGAAAAG CTGAAAGAGA AAACGAATGC AAAAGATGAT
    CCATCCAATA GGATGTCCAG GATGGGAGCA ATGCCTACAC CATTTAAATC AACTGGTGAT
    ATTGCTACAA CTGCTGTCAC TGTAGTAGAT GCAAACAAAG AACCAAAGTA CCAAGATGAT
    GTAGCAGTGC CCCAGCCAAA AGCAGTTACA AGAACGATTC TTAAACCCAG CCCAGAAGAT
    GAAGCAATAT ATGGTCCTAA TACAAAAATG GTAAGATTCA AGAAGGGGGA CAGTGTTGGT
    CTGCGACTTG CTGGTGGAAA TGATGTAGGG ATATTTATTG CTGGAATCCA AGAAGGCACC
    TCAGCTGACC AGGAAGGACT TCAGGAAGGA GATCAGATTC TCAAGGTAAA CGCTCAAGAT
    TTCAGAGGCA TTGTTCGGGA GGATGCTGTT TTATACCTAC TAGAAATTCC CAAAGGTGAT
    ACAGTGACAA TTTTGGCTCA AAGCAAATAT GAAGTATACA GAGACATCAT GGCCTGTGGC
    AGAGGAGATT CATTCTTCAT CAGAAGCCAC TTTGAGTGTG AAAAAGAGTC ACCACAGAGC
    TTAGCCTTCA CCAGAGGGGA GATCTTTAGA GTAGTTGATA CTCTGTATGA TGGCAAACTA
    GGAAACTGGC TGGCTGTGAG AATTGGAAAT GAACTTGAAA AGGGCCTCAT TCCAAACAGG
    AGCAGAGCTG AGCAGATGGC CAGTGTTCAG AATGCCCAGA AAGATGGCCC TAGTGACAGG
    GCAGACTTCT GGAGAACACG CGGCCAGCGA TCTGGAGTGA AGAAGAATTT GAGGAAGAGT
    CGTGAAGATC TGACAGCAAT TGTATCAGTG GGCACAAAAT TCCCAGCTTA TGAACGAGTT
    CAGTTGCGTG AAGCTGGTTT TAAGAGACCT GTTGTGATAT TTGGCCCCAT TGCAGATGTT
    GCTATGGAGA AGTTGTCAAC TGACTTGCCT CACCTGTACC AGACTGCAAA GACAGAACCC
    AGAGATGCAG GTTCAGAGAA GGCAACTGGG GTAGTACGCT TGAACACTGT GAGGCAAATC
    ATTGAGCAGG ATAAACATGC TCTCTTGGAT GTGACTCCTA AAGCAGTGGA TCTGCTGAAT
    TATACCCAGT GGTTTCCCAT TGTAGTCTTC TTTAACCCAG ACAGTAAGCA GGGCGTGAAA
    ACGATGAGAC AAAGGCTGTG TTCCACATCT AACAAAAGCT CGAGGAAGCT TTATGAGCAA
    GCAAACAAGC TGAAGAAGAC TTGTTCCCAC CTCTTTACAG CCACCATCAA TTTGAATTCA
    GCTAATGATA GCTGGTATGG TAGTCTGAAG GATACAATTC AGCAACAGCA AGGTGAAGCA
    GTTTGGGTGT CAGAAGGAAA GGTAGATGGC ATGGAAGATG ACGCAGATGA TCGCATGTCT
    TACCTTACTG CCATGGGTGC TGACTATTTG AGTTGTGACA GTCGGTTGAT CAGTGACTTA
    GAAGATACAG ATGGAGAGGG AGGTGCATAC ACTGATAATG AACTTGATGA ACCTTTGGAT
    GAACCAAGAA TCTCATCTGT TGGCCGGTCC TCTGAACCTG TACATCATGA AGAGAATATA
    AAGAAATTTA GCTTGGAACA AAGAACTCCA TTGAGGAAAG CTGGTAGCAG GGAGATCCTT
    AGAGAACCTA GTCCACCTCC AGCATTCAAG CCTGAACCAC CAAAGGGGAA GTTACAAAAC
    AAAGAGGATC TGTATGACTT CCCTAAGAAC TATGACTCCA AACCAAGTAG CATCCATGTC
    AGCAGTGAGA CTTCAACTGT ATCAGCCAAA GCAGCACCAC CACCTGTTTC TGTGAAACCT
    ACTTTTGGGC GTCCCATCCT GAGAAATACT CAGCCAGCAG TCCCAAGTGC AGAAGAAGAA
    GAGGAGGGAA AGTTGGAAGA GGAAGGGAGT GAACAAGAAA ATACTCCAAA ATCTGTGCTG
    AGGAAAGTAA AAATATTTGA GGAGATGGAT CATAAGGCAA GACTGCAAAG AATGCAAGAG
    CTACAGGAGG CCCAGAATGC CAGGATTGAA ATAGCCCAGA AGCATCCAGA CATTTATGCT
    GTCCCTGTCA AAACACAGAA GTCAGACCAG AACTGGCCCC AGCCCATCAG CTGCCGACCT
    CCAGAACCCC AGAAACCTCC TACCAGACCT TACCTGGAGG ATCGTGGCAG CTGTGGCAGT
    GATGGAGAGG AGGAGGAGGA GTACCGTCGG CAGCTATCAG ACCACTCTAA GAAAGGTTAC
    TACGGACAGC CACCCAGATA CAGAGACACA GAACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48037
    Clone ID Related Accession (Same CDS sequence) XM_015280243.2 , XM_015280243.1
    Accession Version XM_015280243.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3579bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAATGA AGACAGCGCA AGCTCTGCAG AGAATGTGGT CACATGCAGT CAAAAAGTTG 
    AGGATTTTAA GGGGGCACGC CCCAGGCATG GAAGAGCTGA TATGGGAACA GTACACTGTG
    ACCTTACAAA AGGATTCAAA ACGAGGATTT GGGATTGCAG TTTCTGGTGG CAGAGATAAC
    CCACATTTTG AAAATGGTGA AACCTCAATA GTCATTTCAG ATGTTCTCCC AGGTGGCCCA
    GCAGATGGAT TACTTCAAGA AAATGACCGC GTGGTGATTG TTAATGGGAC CCCAATGGAA
    AATGTTCCAC ATTCTTTTGC AGTACAACAG CTTAGGAAAA GTGGAAAAGT GGCCACCATT
    GTTGTGAAAA GACCACGGAA AGTGCAGGCT GCTGCTTTGA GGAGAAGCCC CTCCCTTGAC
    TATGAGGACA GAGCTTTAGA TGTAATGGAT GACCATGCAG AATTTGATGG CAAAAGTGCT
    CGAAGTGGCT ACAGTGAAAG AAGCTGGCGT AGTGGCAATG GAGGGCGCAG TCAAAGCTGG
    GGAAACAGCC TGGATCAGAG CTATAGAGAT GATCAAGACA GAGAGCACAA CCGAAGCAGA
    GGTCGTAGCA GGGAATGTAG CTACAGCCGT GATCAAAGTC GTGGTAGAAG CATTGATAGG
    AGCTTGGATC AAGACTATGG CAGAGATCGG AGCAGGGGAA GAAGCGTTGA CAGGGATGGT
    GGCTATGGAC AGGACTACAG AAGAGACTAC AGTCCAGCCA GTTACAGACA TGGATCTCAA
    CCTGATCCTA GATATGTGAG GGAGGTGAGA AGCCTAAGTC GAGACAGGCT TCATTCTCGT
    AGTCCTTCAC CTGAAACACG CCATCAGCAT GAGTACCTAG GACCACAGGA TCAGAATGGA
    CCCATCAGTG TTCTCTTAAC AAAAGGCAAA CATAATGAAG AATATGGTCT CCGACTTGGA
    AGTCAGATCT TCATAAAAGA AATGACCAGT ACTGGCCTAG CAACCAAAGA TGGCAACCTT
    CATGAAGGGG ATATCATTCT TAAGATCAAT GGTACAGTGA CAGAGAACAT GTCTTTAGCT
    GATGCCCGAA AATTGATTGA GAAGTCTCGG GGAAAGCTCC AGCTGGTTGT CCTCAGGGAC
    AGAAAGCAGA CCCTGCTCAA CATTCCTTCA TTGAATGACA GTGACTCAGA AATGGATGAT
    ATTTCTGAAA TAGAGTCAAA CAGATCGTTC TCCCCTCAAG ATGATAGATT GCATCATTCT
    GATCTGGATT TGCATTCATC CAATGAAAAG CTGAAAGAGA AAACGAATGC AAAAGATGAT
    CCATCCAATA GGATGTCCAG GATGGGAGCA ATGCCTACAC CATTTAAATC AACTGGTGAT
    ATTGCTACAA CTGCTGTCAC TGTAGTAGAT GCAAACAAAG AACCAAAGTA CCAAGATGAT
    GTAGCAGTGC CCCAGCCAAA AGCAGTTACA AGAACGATTC TTAAACCCAG CCCAGAAGAT
    GAAGCAATAT ATGGTCCTAA TACAAAAATG GTAAGATTCA AGAAGGGGGA CAGTGTTGGT
    CTGCGACTTG CTGGTGGAAA TGATGTAGGG ATATTTATTG CTGGAATCCA AGAAGGCACC
    TCAGCTGACC AGGAAGGACT TCAGGAAGGA GATCAGATTC TCAAGGTAAA CGCTCAAGAT
    TTCAGAGGCA TTGTTCGGGA GGATGCTGTT TTATACCTAC TAGAAATTCC CAAAGGTGAT
    ACAGTGACAA TTTTGGCTCA AAGCAAATAT GAAGTATACA GAGACATCAT GGCCTGTGGC
    AGAGGAGATT CATTCTTCAT CAGAAGCCAC TTTGAGTGTG AAAAAGAGTC ACCACAGAGC
    TTAGCCTTCA CCAGAGGGGA GATCTTTAGA GTAGTTGATA CTCTGTATGA TGGCAAACTA
    GGAAACTGGC TGGCTGTGAG AATTGGAAAT GAACTTGAAA AGGGCCTCAT TCCAAACAGG
    AGCAGAGCTG AGCAGATGGC CAGTGTTCAG AATGCCCAGA AAGATGGCCC TAGTGACAGG
    GCAGACTTCT GGAGAACACG CGGCCAGCGA TCTGGAGTGA AGAAGAATTT GAGGAAGAGT
    CGTGAAGATC TGACAGCAAT TGTATCAGTG GGCACAAAAT TCCCAGCTTA TGAACGAGTT
    CAGTTGCGTG AAGCTGGTTT TAAGAGACCT GTTGTGATAT TTGGCCCCAT TGCAGATGTT
    GCTATGGAGA AGTTGTCAAC TGACTTGCCT CACCTGTACC AGACTGCAAA GACAGAACCC
    AGAGATGCAG GTTCAGAGAA GGCAACTGGG GTAGTACGCT TGAACACTGT GAGGCAAATC
    ATTGAGCAGG ATAAACATGC TCTCTTGGAT GTGACTCCTA AAGCAGTGGA TCTGCTGAAT
    TATACCCAGT GGTTTCCCAT TGTAGTCTTC TTTAACCCAG ACAGTAAGCA GGGCGTGAAA
    ACGATGAGAC AAAGGCTGTG TTCCACATCT AACAAAAGCT CGAGGAAGCT TTATGAGCAA
    GCAAACAAGC TGAAGAAGAC TTGTTCCCAC CTCTTTACAG CCACCATCAA TTTGAATTCA
    GCTAATGATA GCTGGTATGG TAGTCTGAAG GATACAATTC AGCAACAGCA AGGTGAAGCA
    GTTTGGGTGT CAGAAGGAAA GGTAGATGGC ATGGAAGATG ACGCAGATGA TCGCATGTCT
    TACCTTACTG CCATGGGTGC TGACTATTTG AGTTGTGACA GTCGGTTGAT CAGTGACTTA
    GAAGATACAG ATGGAGAGGG AGGTGCATAC ACTGATAATG AACTTGATGA ACCTTTGGAT
    GAACCAAGAA TCTCATCTGT TGGCCGGTCC TCTGAACCTG TACATCATGA AGAGAATATA
    AAGAAATTTA GCTTGGAACA AAGAACTCCA TTGAGGAAAG CTGGTAGCAG GGAGATCCTT
    AGAGAACCTA GTCCACCTCC AGCATTCAAG CCTGAACCAC CAAAGGGGAA GTTACAAAAC
    AAAGAGGATC TGTATGACTT CCCTAAGAAC TATGACTCCA AACCAAGTAG CATCCATGTC
    AGCAGTGAGA CTTCAACTGT ATCAGCCAAA GCAGCACCAC CACCTGTTTC TGTGAAACCT
    ACTTTTGGGC GTCCCATCCT GAGAAATACT CAGCCAGCAG TCCCAAGTGC AGAAGAAGAA
    GAGGAGGGAA AGTTGGAAGA GGAAGGGAGT GAACAAGAAA ATACTCCAAA ATCTGTGCTG
    AGGAAAGTAA AAATATTTGA GGAGATGGAT CATAAGGCAA GACTGCAAAG AATGCAAGAG
    CTACAGGAGG CCCAGAATGC CAGGATTGAA ATAGCCCAGA AGCATCCAGA CATTTATGCT
    GTCCCTGTCA AAACACAGAA GTCAGACCAG AACTGGCCCC AGCCCATCAG CTGCCGACCT
    CCAGAACCCC AGAAACCTCC TACCAGACCT TACCTGGAGG ATCGTGGCAG CTGTGGCAGT
    GATGGAGAGG AGGAGGAGGA GTACCGTCGG CAGCTATCAG ACCACTCTAA GAAAGGTTAC
    TACGGACAGC CACCCAGATA CAGAGACACA GAACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135729.1
    CDS10..3588
    Translation

    Target ORF information:

    RefSeq Version XM_015280243.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACAATGA AGACAGCGCA AGCTCTGCAG AGAATGTGGT CACATGCAGT CAAAAAGTTG 
    AGGATTTTAA GGGGGCACGC CCCAGGCATG GAAGAGCTGA TATGGGAACA GTACACTGTG
    ACCTTACAAA AGGATTCAAA ACGAGGATTT GGGATTGCAG TTTCTGGTGG CAGAGATAAC
    CCACATTTTG AAAATGGTGA AACCTCAATA GTCATTTCAG ATGTTCTCCC AGGTGGCCCA
    GCAGATGGAT TACTTCAAGA AAATGACCGC GTGGTGATTG TTAATGGGAC CCCAATGGAA
    AATGTTCCAC ATTCTTTTGC AGTACAACAG CTTAGGAAAA GTGGAAAAGT GGCCACCATT
    GTTGTGAAAA GACCACGGAA AGTGCAGGCT GCTGCTTTGA GGAGAAGCCC CTCCCTTGAC
    TATGAGGACA GAGCTTTAGA TGTAATGGAT GACCATGCAG AATTTGATGG CAAAAGTGCT
    CGAAGTGGCT ACAGTGAAAG AAGCTGGCGT AGTGGCAATG GAGGGCGCAG TCAAAGCTGG
    GGAAACAGCC TGGATCAGAG CTATAGAGAT GATCAAGACA GAGAGCACAA CCGAAGCAGA
    GGTCGTAGCA GGGAATGTAG CTACAGCCGT GATCAAAGTC GTGGTAGAAG CATTGATAGG
    AGCTTGGATC AAGACTATGG CAGAGATCGG AGCAGGGGAA GAAGCGTTGA CAGGGATGGT
    GGCTATGGAC AGGACTACAG AAGAGACTAC AGTCCAGCCA GTTACAGACA TGGATCTCAA
    CCTGATCCTA GATATGTGAG GGAGGTGAGA AGCCTAAGTC GAGACAGGCT TCATTCTCGT
    AGTCCTTCAC CTGAAACACG CCATCAGCAT GAGTACCTAG GACCACAGGA TCAGAATGGA
    CCCATCAGTG TTCTCTTAAC AAAAGGCAAA CATAATGAAG AATATGGTCT CCGACTTGGA
    AGTCAGATCT TCATAAAAGA AATGACCAGT ACTGGCCTAG CAACCAAAGA TGGCAACCTT
    CATGAAGGGG ATATCATTCT TAAGATCAAT GGTACAGTGA CAGAGAACAT GTCTTTAGCT
    GATGCCCGAA AATTGATTGA GAAGTCTCGG GGAAAGCTCC AGCTGGTTGT CCTCAGGGAC
    AGAAAGCAGA CCCTGCTCAA CATTCCTTCA TTGAATGACA GTGACTCAGA AATGGATGAT
    ATTTCTGAAA TAGAGTCAAA CAGATCGTTC TCCCCTCAAG ATGATAGATT GCATCATTCT
    GATCTGGATT TGCATTCATC CAATGAAAAG CTGAAAGAGA AAACGAATGC AAAAGATGAT
    CCATCCAATA GGATGTCCAG GATGGGAGCA ATGCCTACAC CATTTAAATC AACTGGTGAT
    ATTGCTACAA CTGCTGTCAC TGTAGTAGAT GCAAACAAAG AACCAAAGTA CCAAGATGAT
    GTAGCAGTGC CCCAGCCAAA AGCAGTTACA AGAACGATTC TTAAACCCAG CCCAGAAGAT
    GAAGCAATAT ATGGTCCTAA TACAAAAATG GTAAGATTCA AGAAGGGGGA CAGTGTTGGT
    CTGCGACTTG CTGGTGGAAA TGATGTAGGG ATATTTATTG CTGGAATCCA AGAAGGCACC
    TCAGCTGACC AGGAAGGACT TCAGGAAGGA GATCAGATTC TCAAGGTAAA CGCTCAAGAT
    TTCAGAGGCA TTGTTCGGGA GGATGCTGTT TTATACCTAC TAGAAATTCC CAAAGGTGAT
    ACAGTGACAA TTTTGGCTCA AAGCAAATAT GAAGTATACA GAGACATCAT GGCCTGTGGC
    AGAGGAGATT CATTCTTCAT CAGAAGCCAC TTTGAGTGTG AAAAAGAGTC ACCACAGAGC
    TTAGCCTTCA CCAGAGGGGA GATCTTTAGA GTAGTTGATA CTCTGTATGA TGGCAAACTA
    GGAAACTGGC TGGCTGTGAG AATTGGAAAT GAACTTGAAA AGGGCCTCAT TCCAAACAGG
    AGCAGAGCTG AGCAGATGGC CAGTGTTCAG AATGCCCAGA AAGATGGCCC TAGTGACAGG
    GCAGACTTCT GGAGAACACG CGGCCAGCGA TCTGGAGTGA AGAAGAATTT GAGGAAGAGT
    CGTGAAGATC TGACAGCAAT TGTATCAGTG GGCACAAAAT TCCCAGCTTA TGAACGAGTT
    CAGTTGCGTG AAGCTGGTTT TAAGAGACCT GTTGTGATAT TTGGCCCCAT TGCAGATGTT
    GCTATGGAGA AGTTGTCAAC TGACTTGCCT CACCTGTACC AGACTGCAAA GACAGAACCC
    AGAGATGCAG GTTCAGAGAA GGCAACTGGG GTAGTACGCT TGAACACTGT GAGGCAAATC
    ATTGAGCAGG ATAAACATGC TCTCTTGGAT GTGACTCCTA AAGCAGTGGA TCTGCTGAAT
    TATACCCAGT GGTTTCCCAT TGTAGTCTTC TTTAACCCAG ACAGTAAGCA GGGCGTGAAA
    ACGATGAGAC AAAGGCTGTG TTCCACATCT AACAAAAGCT CGAGGAAGCT TTATGAGCAA
    GCAAACAAGC TGAAGAAGAC TTGTTCCCAC CTCTTTACAG CCACCATCAA TTTGAATTCA
    GCTAATGATA GCTGGTATGG TAGTCTGAAG GATACAATTC AGCAACAGCA AGGTGAAGCA
    GTTTGGGTGT CAGAAGGAAA GGTAGATGGC ATGGAAGATG ACGCAGATGA TCGCATGTCT
    TACCTTACTG CCATGGGTGC TGACTATTTG AGTTGTGACA GTCGGTTGAT CAGTGACTTA
    GAAGATACAG ATGGAGAGGG AGGTGCATAC ACTGATAATG AACTTGATGA ACCTTTGGAT
    GAACCAAGAA TCTCATCTGT TGGCCGGTCC TCTGAACCTG TACATCATGA AGAGAATATA
    AAGAAATTTA GCTTGGAACA AAGAACTCCA TTGAGGAAAG CTGGTAGCAG GGAGATCCTT
    AGAGAACCTA GTCCACCTCC AGCATTCAAG CCTGAACCAC CAAAGGGGAA GTTACAAAAC
    AAAGAGGATC TGTATGACTT CCCTAAGAAC TATGACTCCA AACCAAGTAG CATCCATGTC
    AGCAGTGAGA CTTCAACTGT ATCAGCCAAA GCAGCACCAC CACCTGTTTC TGTGAAACCT
    ACTTTTGGGC GTCCCATCCT GAGAAATACT CAGCCAGCAG TCCCAAGTGC AGAAGAAGAA
    GAGGAGGGAA AGTTGGAAGA GGAAGGGAGT GAACAAGAAA ATACTCCAAA ATCTGTGCTG
    AGGAAAGTAA AAATATTTGA GGAGATGGAT CATAAGGCAA GACTGCAAAG AATGCAAGAG
    CTACAGGAGG CCCAGAATGC CAGGATTGAA ATAGCCCAGA AGCATCCAGA CATTTATGCT
    GTCCCTGTCA AAACACAGAA GTCAGACCAG AACTGGCCCC AGCCCATCAG CTGCCGACCT
    CCAGAACCCC AGAAACCTCC TACCAGACCT TACCTGGAGG ATCGTGGCAG CTGTGGCAGT
    GATGGAGAGG AGGAGGAGGA GTACCGTCGG CAGCTATCAG ACCACTCTAA GAAAGGTTAC
    TACGGACAGC CACCCAGATA CAGAGACACA GAACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48038
    Clone ID Related Accession (Same CDS sequence) XM_015280244.1 , XM_015280244.2
    Accession Version XM_015280244.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003764318.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCGA CGCAGGGCTC CGCCGGCAGC GAACCGCCAG GGCCCGCGGG GGAGGCGGAG 
    CAGGCCCCAG GCATGGAAGA GCTGATATGG GAACAGTACA CTGTGACCTT ACAAAAGGAT
    TCAAAACGAG GATTTGGGAT TGCAGTTTCT GGTGGCAGAG ATAACCCACA TTTTGAAAAT
    GGTGAAACCT CAATAGTCAT TTCAGATGTT CTCCCAGGTG GCCCAGCAGA TGGATTACTT
    CAAGAAAATG ACCGCGTGGT GATTGTTAAT GGGACCCCAA TGGAAAATGT TCCACATTCT
    TTTGCAGTAC AACAGCTTAG GAAAAGTGGA AAAGTGGCCA CCATTGTTGT GAAAAGACCA
    CGGAAAGTGC AGGCTGCTGC TTTGAGGAGA AGCCCCTCCC TTGACTATGA GGACAGAGCT
    TTAGATGTAA TGGATGACCA TGCAGAATTT GATGGCAAAA GTGCTCGAAG TGGCTACAGT
    GAAAGAAGCT GGCGTAGTGG CAATGGAGGG CGCAGTCAAA GCTGGGGAAA CAGCCTGGAT
    CAGAGCTATA GAGATGATCA AGACAGAGAG CACAACCGAA GCAGAGGTCG TAGCAGGGAA
    TGTAGCTACA GCCGTGATCA AAGTCGTGGT AGAAGCATTG ATAGGAGCTT GGATCAAGAC
    TATGGCAGAG ATCGGAGCAG GGGAAGAAGC GTTGACAGGG ATGGTGGCTA TGGACAGGAC
    TACAGAAGAG ACTACAGTCC AGCCAGTTAC AGACATGGAT CTCAACCTGA TCCTAGATAT
    GTGAGGGAGG TGAGAAGCCT AAGTCGAGAC AGGCTTCATT CTCGTAGTCC TTCACCTGAA
    ACACGCCATC AGCATGAGTA CCTAGGACCA CAGGATCAGA ATGGACCCAT CAGTGTTCTC
    TTAACAAAAG GCAAACATAA TGAAGAATAT GGTCTCCGAC TTGGAAGTCA GATCTTCATA
    AAAGAAATGA CCAGTACTGG CCTAGCAACC AAAGATGGCA ACCTTCATGA AGGGGATATC
    ATTCTTAAGA TCAATGGTAC AGTGACAGAG AACATGTCTT TAGCTGATGC CCGAAAATTG
    ATTGAGAAGT CTCGGGGAAA GCTCCAGCTG GTTGTCCTCA GGGACAGAAA GCAGACCCTG
    CTCAACATTC CTTCATTGAA TGACAGTGAC TCAGAAATGG ATGATATTTC TGAAATAGAG
    TCAAACAGAT CGTTCTCCCC TCAAGATGAT AGATTGCATC ATTCTGATCT GGATTTGCAT
    TCATCCAATG AAAAGCTGAA AGAGAAAACG AATGCAAAAG ATGATCCATC CAATAGGATG
    TCCAGGATGG GAGCAATGCC TACACCATTT AAATCAACTG GTGATATTGC TACAACTGCT
    GTCACTGTAG TAGATGCAAA CAAAGAACCA AAGTACCAAG ATGATGTAGC AGTGCCCCAG
    CCAAAAGCAG TTACAAGAAC GATTCTTAAA CCCAGCCCAG AAGATGAAGC AATATATGGT
    CCTAATACAA AAATGGTAAG ATTCAAGAAG GGGGACAGTG TTGGTCTGCG ACTTGCTGGT
    GGAAATGATG TAGGGATATT TATTGCTGGA ATCCAAGAAG GCACCTCAGC TGACCAGGAA
    GGACTTCAGG AAGGAGATCA GATTCTCAAG GTAAACGCTC AAGATTTCAG AGGCATTGTT
    CGGGAGGATG CTGTTTTATA CCTACTAGAA ATTCCCAAAG GTGATACAGT GACAATTTTG
    GCTCAAAGCA AATATGAAGT ATACAGAGAC ATCATGGCCT GTGGCAGAGG AGATTCATTC
    TTCATCAGAA GCCACTTTGA GTGTGAAAAA GAGTCACCAC AGAGCTTAGC CTTCACCAGA
    GGGGAGATCT TTAGAGTAGT TGATACTCTG TATGATGGCA AACTAGGAAA CTGGCTGGCT
    GTGAGAATTG GAAATGAACT TGAAAAGGGC CTCATTCCAA ACAGGAGCAG AGCTGAGCAG
    ATGGCCAGTG TTCAGAATGC CCAGAAAGAT GGCCCTAGTG ACAGGGCAGA CTTCTGGAGA
    ACACGCGGCC AGCGATCTGG AGTGAAGAAG AATTTGAGGA AGAGTCGTGA AGATCTGACA
    GCAATTGTAT CAGTGGGCAC AAAATTCCCA GCTTATGAAC GAGTTCAGTT GCGTGAAGCT
    GGTTTTAAGA GACCTGTTGT GATATTTGGC CCCATTGCAG ATGTTGCTAT GGAGAAGTTG
    TCAACTGACT TGCCTCACCT GTACCAGACT GCAAAGACAG AACCCAGAGA TGCAGGTTCA
    GAGAAGGCAA CTGGGGTAGT ACGCTTGAAC ACTGTGAGGC AAATCATTGA GCAGGATAAA
    CATGCTCTCT TGGATGTGAC TCCTAAAGCA GTGGATCTGC TGAATTATAC CCAGTGGTTT
    CCCATTGTAG TCTTCTTTAA CCCAGACAGT AAGCAGGGCG TGAAAACGAT GAGACAAAGG
    CTGTGTTCCA CATCTAACAA AAGCTCGAGG AAGCTTTATG AGCAAGCAAA CAAGCTGAAG
    AAGACTTGTT CCCACCTCTT TACAGCCACC ATCAATTTGA ATTCAGCTAA TGATAGCTGG
    TATGGTAGTC TGAAGGATAC AATTCAGCAA CAGCAAGGTG AAGCAGTTTG GGTGTCAGAA
    GGAAAGGTAG ATGGCATGGA AGATGACGCA GATGATCGCA TGTCTTACCT TACTGCCATG
    GGTGCTGACT ATTTGAGTTG TGACAGTCGG TTGATCAGTG ACTTAGAAGA TACAGATGGA
    GAGGGAGGTG CATACACTGA TAATGAACTT GATGAACCTT TGGATGAACC AAGAATCTCA
    TCTGTTGGCC GGTCCTCTGA ACCTGTACAT CATGAAGAGA ATATAAAGAA ATTTAGCTTG
    GAACAAAGAA CTCCATTGAG GAAAGCTGGT AGCAGGGAGA TCCTTAGAGA ACCTAGTCCA
    CCTCCAGCAT TCAAGCCTGA ACCACCAAAG GGGAAGTTAC AAAACAAAGA GGATCTGTAT
    GACTTCCCTA AGAACTATGA CTCCAAACCA AGTAGCATCC ATGTCAGCAG TGAGACTTCA
    ACTGTATCAG CCAAAGCAGC ACCACCACCT GTTTCTGTGA AACCTACTTT TGGGCGTCCC
    ATCCTGAGAA ATACTCAGCC AGCAGTCCCA AGTGCAGAAG AAGAAGAGGA GGGAAAGTTG
    GAAGAGGAAG GGAGTGAACA AGAAAATACT CCAAAATCTG TGCTGAGGAA AGTAAAAATA
    TTTGAGGAGA TGGATCATAA GGCAAGACTG CAAAGAATGC AAGAGCTACA GGAGGCCCAG
    AATGCCAGGA TTGAAATAGC CCAGAAGCAT CCAGACATTT ATGCTGTCCC TGTCAAAACA
    CAGAAGTCAG ACCAGAACTG GCCCCAGCCC ATCAGCTGCC GACCTCCAGA ACCCCAGAAA
    CCTCCTACCA GACCTTACCT GGAGGATCGT GGCAGCTGTG GCAGTGATGG AGAGGAGGAG
    GAGGAGTACC GTCGGCAGCT ATCAGACCAC TCTAAGAAAG GTTACTACGG ACAGCCACCC
    AGATACAGAG ACACAGAACT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135730.1
    CDS36..3599
    Translation

    Target ORF information:

    RefSeq Version XM_015280244.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280244.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCGA CGCAGGGCTC CGCCGGCAGC GAACCGCCAG GGCCCGCGGG GGAGGCGGAG 
    CAGGCCCCAG GCATGGAAGA GCTGATATGG GAACAGTACA CTGTGACCTT ACAAAAGGAT
    TCAAAACGAG GATTTGGGAT TGCAGTTTCT GGTGGCAGAG ATAACCCACA TTTTGAAAAT
    GGTGAAACCT CAATAGTCAT TTCAGATGTT CTCCCAGGTG GCCCAGCAGA TGGATTACTT
    CAAGAAAATG ACCGCGTGGT GATTGTTAAT GGGACCCCAA TGGAAAATGT TCCACATTCT
    TTTGCAGTAC AACAGCTTAG GAAAAGTGGA AAAGTGGCCA CCATTGTTGT GAAAAGACCA
    CGGAAAGTGC AGGCTGCTGC TTTGAGGAGA AGCCCCTCCC TTGACTATGA GGACAGAGCT
    TTAGATGTAA TGGATGACCA TGCAGAATTT GATGGCAAAA GTGCTCGAAG TGGCTACAGT
    GAAAGAAGCT GGCGTAGTGG CAATGGAGGG CGCAGTCAAA GCTGGGGAAA CAGCCTGGAT
    CAGAGCTATA GAGATGATCA AGACAGAGAG CACAACCGAA GCAGAGGTCG TAGCAGGGAA
    TGTAGCTACA GCCGTGATCA AAGTCGTGGT AGAAGCATTG ATAGGAGCTT GGATCAAGAC
    TATGGCAGAG ATCGGAGCAG GGGAAGAAGC GTTGACAGGG ATGGTGGCTA TGGACAGGAC
    TACAGAAGAG ACTACAGTCC AGCCAGTTAC AGACATGGAT CTCAACCTGA TCCTAGATAT
    GTGAGGGAGG TGAGAAGCCT AAGTCGAGAC AGGCTTCATT CTCGTAGTCC TTCACCTGAA
    ACACGCCATC AGCATGAGTA CCTAGGACCA CAGGATCAGA ATGGACCCAT CAGTGTTCTC
    TTAACAAAAG GCAAACATAA TGAAGAATAT GGTCTCCGAC TTGGAAGTCA GATCTTCATA
    AAAGAAATGA CCAGTACTGG CCTAGCAACC AAAGATGGCA ACCTTCATGA AGGGGATATC
    ATTCTTAAGA TCAATGGTAC AGTGACAGAG AACATGTCTT TAGCTGATGC CCGAAAATTG
    ATTGAGAAGT CTCGGGGAAA GCTCCAGCTG GTTGTCCTCA GGGACAGAAA GCAGACCCTG
    CTCAACATTC CTTCATTGAA TGACAGTGAC TCAGAAATGG ATGATATTTC TGAAATAGAG
    TCAAACAGAT CGTTCTCCCC TCAAGATGAT AGATTGCATC ATTCTGATCT GGATTTGCAT
    TCATCCAATG AAAAGCTGAA AGAGAAAACG AATGCAAAAG ATGATCCATC CAATAGGATG
    TCCAGGATGG GAGCAATGCC TACACCATTT AAATCAACTG GTGATATTGC TACAACTGCT
    GTCACTGTAG TAGATGCAAA CAAAGAACCA AAGTACCAAG ATGATGTAGC AGTGCCCCAG
    CCAAAAGCAG TTACAAGAAC GATTCTTAAA CCCAGCCCAG AAGATGAAGC AATATATGGT
    CCTAATACAA AAATGGTAAG ATTCAAGAAG GGGGACAGTG TTGGTCTGCG ACTTGCTGGT
    GGAAATGATG TAGGGATATT TATTGCTGGA ATCCAAGAAG GCACCTCAGC TGACCAGGAA
    GGACTTCAGG AAGGAGATCA GATTCTCAAG GTAAACGCTC AAGATTTCAG AGGCATTGTT
    CGGGAGGATG CTGTTTTATA CCTACTAGAA ATTCCCAAAG GTGATACAGT GACAATTTTG
    GCTCAAAGCA AATATGAAGT ATACAGAGAC ATCATGGCCT GTGGCAGAGG AGATTCATTC
    TTCATCAGAA GCCACTTTGA GTGTGAAAAA GAGTCACCAC AGAGCTTAGC CTTCACCAGA
    GGGGAGATCT TTAGAGTAGT TGATACTCTG TATGATGGCA AACTAGGAAA CTGGCTGGCT
    GTGAGAATTG GAAATGAACT TGAAAAGGGC CTCATTCCAA ACAGGAGCAG AGCTGAGCAG
    ATGGCCAGTG TTCAGAATGC CCAGAAAGAT GGCCCTAGTG ACAGGGCAGA CTTCTGGAGA
    ACACGCGGCC AGCGATCTGG AGTGAAGAAG AATTTGAGGA AGAGTCGTGA AGATCTGACA
    GCAATTGTAT CAGTGGGCAC AAAATTCCCA GCTTATGAAC GAGTTCAGTT GCGTGAAGCT
    GGTTTTAAGA GACCTGTTGT GATATTTGGC CCCATTGCAG ATGTTGCTAT GGAGAAGTTG
    TCAACTGACT TGCCTCACCT GTACCAGACT GCAAAGACAG AACCCAGAGA TGCAGGTTCA
    GAGAAGGCAA CTGGGGTAGT ACGCTTGAAC ACTGTGAGGC AAATCATTGA GCAGGATAAA
    CATGCTCTCT TGGATGTGAC TCCTAAAGCA GTGGATCTGC TGAATTATAC CCAGTGGTTT
    CCCATTGTAG TCTTCTTTAA CCCAGACAGT AAGCAGGGCG TGAAAACGAT GAGACAAAGG
    CTGTGTTCCA CATCTAACAA AAGCTCGAGG AAGCTTTATG AGCAAGCAAA CAAGCTGAAG
    AAGACTTGTT CCCACCTCTT TACAGCCACC ATCAATTTGA ATTCAGCTAA TGATAGCTGG
    TATGGTAGTC TGAAGGATAC AATTCAGCAA CAGCAAGGTG AAGCAGTTTG GGTGTCAGAA
    GGAAAGGTAG ATGGCATGGA AGATGACGCA GATGATCGCA TGTCTTACCT TACTGCCATG
    GGTGCTGACT ATTTGAGTTG TGACAGTCGG TTGATCAGTG ACTTAGAAGA TACAGATGGA
    GAGGGAGGTG CATACACTGA TAATGAACTT GATGAACCTT TGGATGAACC AAGAATCTCA
    TCTGTTGGCC GGTCCTCTGA ACCTGTACAT CATGAAGAGA ATATAAAGAA ATTTAGCTTG
    GAACAAAGAA CTCCATTGAG GAAAGCTGGT AGCAGGGAGA TCCTTAGAGA ACCTAGTCCA
    CCTCCAGCAT TCAAGCCTGA ACCACCAAAG GGGAAGTTAC AAAACAAAGA GGATCTGTAT
    GACTTCCCTA AGAACTATGA CTCCAAACCA AGTAGCATCC ATGTCAGCAG TGAGACTTCA
    ACTGTATCAG CCAAAGCAGC ACCACCACCT GTTTCTGTGA AACCTACTTT TGGGCGTCCC
    ATCCTGAGAA ATACTCAGCC AGCAGTCCCA AGTGCAGAAG AAGAAGAGGA GGGAAAGTTG
    GAAGAGGAAG GGAGTGAACA AGAAAATACT CCAAAATCTG TGCTGAGGAA AGTAAAAATA
    TTTGAGGAGA TGGATCATAA GGCAAGACTG CAAAGAATGC AAGAGCTACA GGAGGCCCAG
    AATGCCAGGA TTGAAATAGC CCAGAAGCAT CCAGACATTT ATGCTGTCCC TGTCAAAACA
    CAGAAGTCAG ACCAGAACTG GCCCCAGCCC ATCAGCTGCC GACCTCCAGA ACCCCAGAAA
    CCTCCTACCA GACCTTACCT GGAGGATCGT GGCAGCTGTG GCAGTGATGG AGAGGAGGAG
    GAGGAGTACC GTCGGCAGCT ATCAGACCAC TCTAAGAAAG GTTACTACGG ACAGCCACCC
    AGATACAGAG ACACAGAACT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa48038
    Clone ID Related Accession (Same CDS sequence) XM_015280244.1 , XM_015280244.2
    Accession Version XM_015280244.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3564bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015280244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCGA CGCAGGGCTC CGCCGGCAGC GAACCGCCAG GGCCCGCGGG GGAGGCGGAG 
    CAGGCCCCAG GCATGGAAGA GCTGATATGG GAACAGTACA CTGTGACCTT ACAAAAGGAT
    TCAAAACGAG GATTTGGGAT TGCAGTTTCT GGTGGCAGAG ATAACCCACA TTTTGAAAAT
    GGTGAAACCT CAATAGTCAT TTCAGATGTT CTCCCAGGTG GCCCAGCAGA TGGATTACTT
    CAAGAAAATG ACCGCGTGGT GATTGTTAAT GGGACCCCAA TGGAAAATGT TCCACATTCT
    TTTGCAGTAC AACAGCTTAG GAAAAGTGGA AAAGTGGCCA CCATTGTTGT GAAAAGACCA
    CGGAAAGTGC AGGCTGCTGC TTTGAGGAGA AGCCCCTCCC TTGACTATGA GGACAGAGCT
    TTAGATGTAA TGGATGACCA TGCAGAATTT GATGGCAAAA GTGCTCGAAG TGGCTACAGT
    GAAAGAAGCT GGCGTAGTGG CAATGGAGGG CGCAGTCAAA GCTGGGGAAA CAGCCTGGAT
    CAGAGCTATA GAGATGATCA AGACAGAGAG CACAACCGAA GCAGAGGTCG TAGCAGGGAA
    TGTAGCTACA GCCGTGATCA AAGTCGTGGT AGAAGCATTG ATAGGAGCTT GGATCAAGAC
    TATGGCAGAG ATCGGAGCAG GGGAAGAAGC GTTGACAGGG ATGGTGGCTA TGGACAGGAC
    TACAGAAGAG ACTACAGTCC AGCCAGTTAC AGACATGGAT CTCAACCTGA TCCTAGATAT
    GTGAGGGAGG TGAGAAGCCT AAGTCGAGAC AGGCTTCATT CTCGTAGTCC TTCACCTGAA
    ACACGCCATC AGCATGAGTA CCTAGGACCA CAGGATCAGA ATGGACCCAT CAGTGTTCTC
    TTAACAAAAG GCAAACATAA TGAAGAATAT GGTCTCCGAC TTGGAAGTCA GATCTTCATA
    AAAGAAATGA CCAGTACTGG CCTAGCAACC AAAGATGGCA ACCTTCATGA AGGGGATATC
    ATTCTTAAGA TCAATGGTAC AGTGACAGAG AACATGTCTT TAGCTGATGC CCGAAAATTG
    ATTGAGAAGT CTCGGGGAAA GCTCCAGCTG GTTGTCCTCA GGGACAGAAA GCAGACCCTG
    CTCAACATTC CTTCATTGAA TGACAGTGAC TCAGAAATGG ATGATATTTC TGAAATAGAG
    TCAAACAGAT CGTTCTCCCC TCAAGATGAT AGATTGCATC ATTCTGATCT GGATTTGCAT
    TCATCCAATG AAAAGCTGAA AGAGAAAACG AATGCAAAAG ATGATCCATC CAATAGGATG
    TCCAGGATGG GAGCAATGCC TACACCATTT AAATCAACTG GTGATATTGC TACAACTGCT
    GTCACTGTAG TAGATGCAAA CAAAGAACCA AAGTACCAAG ATGATGTAGC AGTGCCCCAG
    CCAAAAGCAG TTACAAGAAC GATTCTTAAA CCCAGCCCAG AAGATGAAGC AATATATGGT
    CCTAATACAA AAATGGTAAG ATTCAAGAAG GGGGACAGTG TTGGTCTGCG ACTTGCTGGT
    GGAAATGATG TAGGGATATT TATTGCTGGA ATCCAAGAAG GCACCTCAGC TGACCAGGAA
    GGACTTCAGG AAGGAGATCA GATTCTCAAG GTAAACGCTC AAGATTTCAG AGGCATTGTT
    CGGGAGGATG CTGTTTTATA CCTACTAGAA ATTCCCAAAG GTGATACAGT GACAATTTTG
    GCTCAAAGCA AATATGAAGT ATACAGAGAC ATCATGGCCT GTGGCAGAGG AGATTCATTC
    TTCATCAGAA GCCACTTTGA GTGTGAAAAA GAGTCACCAC AGAGCTTAGC CTTCACCAGA
    GGGGAGATCT TTAGAGTAGT TGATACTCTG TATGATGGCA AACTAGGAAA CTGGCTGGCT
    GTGAGAATTG GAAATGAACT TGAAAAGGGC CTCATTCCAA ACAGGAGCAG AGCTGAGCAG
    ATGGCCAGTG TTCAGAATGC CCAGAAAGAT GGCCCTAGTG ACAGGGCAGA CTTCTGGAGA
    ACACGCGGCC AGCGATCTGG AGTGAAGAAG AATTTGAGGA AGAGTCGTGA AGATCTGACA
    GCAATTGTAT CAGTGGGCAC AAAATTCCCA GCTTATGAAC GAGTTCAGTT GCGTGAAGCT
    GGTTTTAAGA GACCTGTTGT GATATTTGGC CCCATTGCAG ATGTTGCTAT GGAGAAGTTG
    TCAACTGACT TGCCTCACCT GTACCAGACT GCAAAGACAG AACCCAGAGA TGCAGGTTCA
    GAGAAGGCAA CTGGGGTAGT ACGCTTGAAC ACTGTGAGGC AAATCATTGA GCAGGATAAA
    CATGCTCTCT TGGATGTGAC TCCTAAAGCA GTGGATCTGC TGAATTATAC CCAGTGGTTT
    CCCATTGTAG TCTTCTTTAA CCCAGACAGT AAGCAGGGCG TGAAAACGAT GAGACAAAGG
    CTGTGTTCCA CATCTAACAA AAGCTCGAGG AAGCTTTATG AGCAAGCAAA CAAGCTGAAG
    AAGACTTGTT CCCACCTCTT TACAGCCACC ATCAATTTGA ATTCAGCTAA TGATAGCTGG
    TATGGTAGTC TGAAGGATAC AATTCAGCAA CAGCAAGGTG AAGCAGTTTG GGTGTCAGAA
    GGAAAGGTAG ATGGCATGGA AGATGACGCA GATGATCGCA TGTCTTACCT TACTGCCATG
    GGTGCTGACT ATTTGAGTTG TGACAGTCGG TTGATCAGTG ACTTAGAAGA TACAGATGGA
    GAGGGAGGTG CATACACTGA TAATGAACTT GATGAACCTT TGGATGAACC AAGAATCTCA
    TCTGTTGGCC GGTCCTCTGA ACCTGTACAT CATGAAGAGA ATATAAAGAA ATTTAGCTTG
    GAACAAAGAA CTCCATTGAG GAAAGCTGGT AGCAGGGAGA TCCTTAGAGA ACCTAGTCCA
    CCTCCAGCAT TCAAGCCTGA ACCACCAAAG GGGAAGTTAC AAAACAAAGA GGATCTGTAT
    GACTTCCCTA AGAACTATGA CTCCAAACCA AGTAGCATCC ATGTCAGCAG TGAGACTTCA
    ACTGTATCAG CCAAAGCAGC ACCACCACCT GTTTCTGTGA AACCTACTTT TGGGCGTCCC
    ATCCTGAGAA ATACTCAGCC AGCAGTCCCA AGTGCAGAAG AAGAAGAGGA GGGAAAGTTG
    GAAGAGGAAG GGAGTGAACA AGAAAATACT CCAAAATCTG TGCTGAGGAA AGTAAAAATA
    TTTGAGGAGA TGGATCATAA GGCAAGACTG CAAAGAATGC AAGAGCTACA GGAGGCCCAG
    AATGCCAGGA TTGAAATAGC CCAGAAGCAT CCAGACATTT ATGCTGTCCC TGTCAAAACA
    CAGAAGTCAG ACCAGAACTG GCCCCAGCCC ATCAGCTGCC GACCTCCAGA ACCCCAGAAA
    CCTCCTACCA GACCTTACCT GGAGGATCGT GGCAGCTGTG GCAGTGATGG AGAGGAGGAG
    GAGGAGTACC GTCGGCAGCT ATCAGACCAC TCTAAGAAAG GTTACTACGG ACAGCCACCC
    AGATACAGAG ACACAGAACT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015135730.1
    CDS39..3602
    Translation

    Target ORF information:

    RefSeq Version XM_015280244.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015280244.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGGAGCGA CGCAGGGCTC CGCCGGCAGC GAACCGCCAG GGCCCGCGGG GGAGGCGGAG 
    CAGGCCCCAG GCATGGAAGA GCTGATATGG GAACAGTACA CTGTGACCTT ACAAAAGGAT
    TCAAAACGAG GATTTGGGAT TGCAGTTTCT GGTGGCAGAG ATAACCCACA TTTTGAAAAT
    GGTGAAACCT CAATAGTCAT TTCAGATGTT CTCCCAGGTG GCCCAGCAGA TGGATTACTT
    CAAGAAAATG ACCGCGTGGT GATTGTTAAT GGGACCCCAA TGGAAAATGT TCCACATTCT
    TTTGCAGTAC AACAGCTTAG GAAAAGTGGA AAAGTGGCCA CCATTGTTGT GAAAAGACCA
    CGGAAAGTGC AGGCTGCTGC TTTGAGGAGA AGCCCCTCCC TTGACTATGA GGACAGAGCT
    TTAGATGTAA TGGATGACCA TGCAGAATTT GATGGCAAAA GTGCTCGAAG TGGCTACAGT
    GAAAGAAGCT GGCGTAGTGG CAATGGAGGG CGCAGTCAAA GCTGGGGAAA CAGCCTGGAT
    CAGAGCTATA GAGATGATCA AGACAGAGAG CACAACCGAA GCAGAGGTCG TAGCAGGGAA
    TGTAGCTACA GCCGTGATCA AAGTCGTGGT AGAAGCATTG ATAGGAGCTT GGATCAAGAC
    TATGGCAGAG ATCGGAGCAG GGGAAGAAGC GTTGACAGGG ATGGTGGCTA TGGACAGGAC
    TACAGAAGAG ACTACAGTCC AGCCAGTTAC AGACATGGAT CTCAACCTGA TCCTAGATAT
    GTGAGGGAGG TGAGAAGCCT AAGTCGAGAC AGGCTTCATT CTCGTAGTCC TTCACCTGAA
    ACACGCCATC AGCATGAGTA CCTAGGACCA CAGGATCAGA ATGGACCCAT CAGTGTTCTC
    TTAACAAAAG GCAAACATAA TGAAGAATAT GGTCTCCGAC TTGGAAGTCA GATCTTCATA
    AAAGAAATGA CCAGTACTGG CCTAGCAACC AAAGATGGCA ACCTTCATGA AGGGGATATC
    ATTCTTAAGA TCAATGGTAC AGTGACAGAG AACATGTCTT TAGCTGATGC CCGAAAATTG
    ATTGAGAAGT CTCGGGGAAA GCTCCAGCTG GTTGTCCTCA GGGACAGAAA GCAGACCCTG
    CTCAACATTC CTTCATTGAA TGACAGTGAC TCAGAAATGG ATGATATTTC TGAAATAGAG
    TCAAACAGAT CGTTCTCCCC TCAAGATGAT AGATTGCATC ATTCTGATCT GGATTTGCAT
    TCATCCAATG AAAAGCTGAA AGAGAAAACG AATGCAAAAG ATGATCCATC CAATAGGATG
    TCCAGGATGG GAGCAATGCC TACACCATTT AAATCAACTG GTGATATTGC TACAACTGCT
    GTCACTGTAG TAGATGCAAA CAAAGAACCA AAGTACCAAG ATGATGTAGC AGTGCCCCAG
    CCAAAAGCAG TTACAAGAAC GATTCTTAAA CCCAGCCCAG AAGATGAAGC AATATATGGT
    CCTAATACAA AAATGGTAAG ATTCAAGAAG GGGGACAGTG TTGGTCTGCG ACTTGCTGGT
    GGAAATGATG TAGGGATATT TATTGCTGGA ATCCAAGAAG GCACCTCAGC TGACCAGGAA
    GGACTTCAGG AAGGAGATCA GATTCTCAAG GTAAACGCTC AAGATTTCAG AGGCATTGTT
    CGGGAGGATG CTGTTTTATA CCTACTAGAA ATTCCCAAAG GTGATACAGT GACAATTTTG
    GCTCAAAGCA AATATGAAGT ATACAGAGAC ATCATGGCCT GTGGCAGAGG AGATTCATTC
    TTCATCAGAA GCCACTTTGA GTGTGAAAAA GAGTCACCAC AGAGCTTAGC CTTCACCAGA
    GGGGAGATCT TTAGAGTAGT TGATACTCTG TATGATGGCA AACTAGGAAA CTGGCTGGCT
    GTGAGAATTG GAAATGAACT TGAAAAGGGC CTCATTCCAA ACAGGAGCAG AGCTGAGCAG
    ATGGCCAGTG TTCAGAATGC CCAGAAAGAT GGCCCTAGTG ACAGGGCAGA CTTCTGGAGA
    ACACGCGGCC AGCGATCTGG AGTGAAGAAG AATTTGAGGA AGAGTCGTGA AGATCTGACA
    GCAATTGTAT CAGTGGGCAC AAAATTCCCA GCTTATGAAC GAGTTCAGTT GCGTGAAGCT
    GGTTTTAAGA GACCTGTTGT GATATTTGGC CCCATTGCAG ATGTTGCTAT GGAGAAGTTG
    TCAACTGACT TGCCTCACCT GTACCAGACT GCAAAGACAG AACCCAGAGA TGCAGGTTCA
    GAGAAGGCAA CTGGGGTAGT ACGCTTGAAC ACTGTGAGGC AAATCATTGA GCAGGATAAA
    CATGCTCTCT TGGATGTGAC TCCTAAAGCA GTGGATCTGC TGAATTATAC CCAGTGGTTT
    CCCATTGTAG TCTTCTTTAA CCCAGACAGT AAGCAGGGCG TGAAAACGAT GAGACAAAGG
    CTGTGTTCCA CATCTAACAA AAGCTCGAGG AAGCTTTATG AGCAAGCAAA CAAGCTGAAG
    AAGACTTGTT CCCACCTCTT TACAGCCACC ATCAATTTGA ATTCAGCTAA TGATAGCTGG
    TATGGTAGTC TGAAGGATAC AATTCAGCAA CAGCAAGGTG AAGCAGTTTG GGTGTCAGAA
    GGAAAGGTAG ATGGCATGGA AGATGACGCA GATGATCGCA TGTCTTACCT TACTGCCATG
    GGTGCTGACT ATTTGAGTTG TGACAGTCGG TTGATCAGTG ACTTAGAAGA TACAGATGGA
    GAGGGAGGTG CATACACTGA TAATGAACTT GATGAACCTT TGGATGAACC AAGAATCTCA
    TCTGTTGGCC GGTCCTCTGA ACCTGTACAT CATGAAGAGA ATATAAAGAA ATTTAGCTTG
    GAACAAAGAA CTCCATTGAG GAAAGCTGGT AGCAGGGAGA TCCTTAGAGA ACCTAGTCCA
    CCTCCAGCAT TCAAGCCTGA ACCACCAAAG GGGAAGTTAC AAAACAAAGA GGATCTGTAT
    GACTTCCCTA AGAACTATGA CTCCAAACCA AGTAGCATCC ATGTCAGCAG TGAGACTTCA
    ACTGTATCAG CCAAAGCAGC ACCACCACCT GTTTCTGTGA AACCTACTTT TGGGCGTCCC
    ATCCTGAGAA ATACTCAGCC AGCAGTCCCA AGTGCAGAAG AAGAAGAGGA GGGAAAGTTG
    GAAGAGGAAG GGAGTGAACA AGAAAATACT CCAAAATCTG TGCTGAGGAA AGTAAAAATA
    TTTGAGGAGA TGGATCATAA GGCAAGACTG CAAAGAATGC AAGAGCTACA GGAGGCCCAG
    AATGCCAGGA TTGAAATAGC CCAGAAGCAT CCAGACATTT ATGCTGTCCC TGTCAAAACA
    CAGAAGTCAG ACCAGAACTG GCCCCAGCCC ATCAGCTGCC GACCTCCAGA ACCCCAGAAA
    CCTCCTACCA GACCTTACCT GGAGGATCGT GGCAGCTGTG GCAGTGATGG AGAGGAGGAG
    GAGGAGTACC GTCGGCAGCT ATCAGACCAC TCTAAGAAAG GTTACTACGG ACAGCCACCC
    AGATACAGAG ACACAGAACT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00603
    Clone ID Related Accession (Same CDS sequence) NM_204918.1
    Accession Version NM_204918.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-20
    Organism Gallus gallus(chicken)
    Product tight junction protein ZO-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF085184.1. ##Evidence-Data-START## Transcript exon combination :: AF085184.1 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAAAAA CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGAAGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GTAGGGGATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGGCTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_990249.1
    CDS220..3711
    Translation

    Target ORF information:

    RefSeq Version NM_204918.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus tight junction protein 2 (TJP2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCTTAC AAAAGGATTC AAAACGAGGA 
    TTTGGGATTG CAGTTTCTGG TGGCAGAGAT AACCCACATT TTGAAAATGG TGAAACCTCA
    ATAGTCATTT CAGATGTTCT CCCAGGTGGC CCAGCAGATG GATTACTTCA AGAAAATGAC
    CGCGTGGTGA TTGTTAATGG GACCCCAATG GAAAATGTTC CACATTCTTT TGCAGTACAA
    CAGCTTAGGA AAAGTGGAAA AGTGGCCACC ATTGTTGTGA AAAGACCACG GAAAGTGCAG
    GCTGCTGCTT TGAGGAAAAA CCCCTCCCTT GACTATGAGG ACAGAGCTTT AGAAGTAATG
    GATGACCATG CAGAATTTGA TGGCAAAAGT GCTCGAAGTG GCTACAGTGA AAGAAGCTGG
    CGTAGTGGCA ATGGAGGGCG CAGTCAAAGC TGGGGAAACA GCCTGGATCA GAGCTATAGA
    GATGATCAAG ACAGAGAGCA CAACCGAAGC AGAGGTCGTA GTAGGGGATG TAGCTACAGC
    CGTGATCAAA GTCGTGGTAG AAGCATTGAT AGGAGCTTGG ATCAAGACTA TGGCAGAGAT
    CGGAGCAGGG GAAGAAGCGT TGACAGGGAT GGTGGCTATG GACAGGACTA CAGAAGAGAC
    TACAGTCCAG CCAGTTACAG ACATGGATCT CAACCTGATC CTAGATATGT GAGGGAGGTG
    AGAAGCCTAA GTCGAGACAG GCTTCATTCT CGTAGTCCTT CACCTGAAAC ACGCCATCAG
    CATGAGTACC TAGGACCACA GGATCAGAAT GGACCCATCA GTGTTCTCTT AACAAAAGGC
    AAACATAATG AAGAATATGG TCTCCGACTT GGAAGTCAGA TCTTCATAAA AGAAATGACC
    AGTACTGGCC TAGCAACCAA AGATGGCAAC CTTCATGAAG GGGATATCAT TCTTAAGATC
    AATGGTACAG TGACAGAGAA CATGTCTTTA GCTGATGCCC GAAAATTGAT TGAGAAGTCT
    CGGGGAAAGC TCCAGCTGGT TGTCCTCAGG GACAGAAAGC AGACCCTGCT CAACATTCCT
    TCATTGAATG ACAGTGACTC AGAAATGGAT GATATTTCTG AAATAGAGTC AAACAGATCG
    TTCTCCCCTC AAGATGATAG ATTGCATCAT TCTGATCTGG ATTTGCATTC ATCCAATGAA
    AAGCTGAAAG AGAAAACGAA TGCAAAAGAT GATCCATCCA ATAGGATGTC CAGGATGGGA
    GCAATGCCTA CACCATTTAA ATCAACTGGT GATATTGCTA CAACTGCTGT CACTGTAGTA
    GATGCAAACA AAGAACCAAA GTACCAAGAT GATGTAGCAG TGCCCCAGCC AAAAGCAGTT
    ACAAGAACGA TTCTTAAACC CAGCCCAGAA GATGAAGCAA TATATGGTCC TAATACAAAA
    ATGGTAAGAT TCAAGAAGGG GGACAGTGTT GGTCTGCGAC TTGCTGGTGG AAATGATGTA
    GGGATATTTA TTGCTGGAAT CCAAGAAGGC ACCTCAGCTG ACCAGGAAGG ACTTCAGGAA
    GGAGATCAGA TTCTCAAGGT AAACGCTCAA GATTTCAGAG GCATTGTTCG GGAGGATGCT
    GTTTTATACC TACTAGAAAT TCCCAAAGGT GATACAGTGA CAATTTTGGC TCAAAGCAAA
    TATGAAGTAT ACAGAGACAT CATGGCCTGT GGCAGAGGAG ATTCATTCTT CATCAGAAGC
    CACTTTGAGT GTGAAAAAGA GTCACCACAG AGCTTAGCCT TCACCAGAGG GGAGATCTTT
    AGAGTAGTTG ATACTCTGTA TGATGGCAAA CTAGGAAACT GGCTGGCTGT GAGAATTGGA
    AATGAACTTG AAAAGGGCCT CATTCCAAAC AGGAGCAGAG CTGAGCAGAT GGCCAGTGTT
    CAGAATGCCC AGAAAGATGG CCCTAGTGAC AGGGCAGACT TCTGGAGAAC ACGCGGCCAG
    CGATCTGGAG TGAAGAAGAA TTTGAGGAAG AGTCGTGAAG ATCTGACAGC AATTGTATCA
    GTGGGCACAA AATTCCCAGC TTATGAACGA GTTCAGTTGC GTGAAGCTGG TTTTAAGAGA
    CCTGTTGTGA TATTTGGCCC CATTGCAGAT GTTGCTATGG AGAAGTTGTC AACTGACTTG
    CCTCACCTGT ACCAGACTGC AAAGACAGAA CCCAGAGATG CAGGTTCAGA GAAGGCAACT
    GGGGTAGTAC GCTTGAACAC TGTGAGGCAA ATCATTGAGC AGGATAAACA TGCTCTCTTG
    GATGTGACTC CTAAAGCAGT GGATCTGCTG AATTATACCC AGTGGTTTCC CATTGTAGTC
    TTCTTTAACC CAGACAGTAA GCAGGGCGTG AAAACGATGA GACAAAGGCT GTGTTCCACA
    TCTAACAAAA GCTCGAGGAA GCTTTATGAG CAAGCAAACA AGCTGAAGAA GACTTGTTCC
    CACCTCTTTA CAGCCACCAT CAATTTGAAT TCAGCTAATG ATAGCTGGTA TGGTAGTCTG
    AAGGATACAA TTCAGCAACA GCAAGGTGAA GCAGTTTGGG TGTCAGAAGG AAAGGTAGAT
    GGCATGGAAG ATGACGCAGA TGATCGCATG TCTTACCTTA CTGCCATGGG TGCTGACTAT
    TTGAGTTGTG ACAGTCGGTT GATCAGTGAC TTAGAAGATA CAGATGGAGA GGGAGGTGCA
    TACACTGATA ATGAACTTGA TGAACCTTTG GATGAACCAA GAATCTCATC TGTTGGCCGG
    TCCTCTGAAC CTGTACATCA TGAAGAGAAT ATAAAGAAAT TTAGCTTGGA ACAAAGAACT
    CCATTGAGGA AAGCTGGTAG CAGGGAGATC CTTAGAGAAC CTAGTCCACC TCCAGCATTC
    AAGCCTGAAC CACCAAAGGG GAAGTTACAA AACAAAGAGG ATCTGTATGA CTTCCCTAAG
    AACTATGACT CCAAACCAAG TAGCATCCAT GTCAGCAGTG AGACTTCAAC TGTATCAGCC
    AAAGCAGCAC CACCACCTGT TTCTGTGAAA CCTACTTTTG GGCGTCCCAT CCTGAGAAAT
    ACTCAGCCAG CAGTCCCAAG TGCAGAAGAA GAAGAGGAGG GAAAGTTGGA AGAGGAAGGG
    AGTGAACAAG AAAATACTCC AAAATCTGTG CTGAGGAAAG TAAAAATATT TGAGGAGATG
    GATCATAAGG CAAGGCTGCA AAGAATGCAA GAGCTACAGG AGGCCCAGAA TGCCAGGATT
    GAAATAGCCC AGAAGCATCC AGACATTTAT GCTGTCCCTG TCAAAACACA GAAGTCAGAC
    CAGAACTGGC CCCAGCCCAT CAGCTGCCGA CCTCCAGAAC CCCAGAAACC TCCTACCAGA
    CCTTACCTGG AGGATCGTGG CAGCTGTGGC AGTGATGGAG AGGAGGAGGA GGAGTACCGT
    CGGCAGCTAT CAGACCACTC TAAGAAAGGT TACTACGGAC AGCCACCCAG ATACAGAGAC
    ACAGAACTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.