CHRNA7 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRNA7
Entrez Gene ID	374001
Full Name	cholinergic receptor nicotinic alpha 7 subunit
General protein information	Preferred Names cholinergic receptor nicotinic alpha 7 subunit Names neuronal acetylcholine receptor subunit alpha-7 cholinergic receptor, nicotinic, alpha 7 (neuronal) cholinergic receptor, nicotinic, alpha polypeptide 7
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204181.2	NP_989512.2	neuronal acetylcholine receptor subunit alpha-7 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chrna7	NP_031416.3
Rattus norvegicus (Norway rat)	Chrna7	NP_036964.3
Drosophila melanogaster (fruit fly)	nAcRalpha-34E	NP_001246027.1
Caenorhabditis elegans (roundworm)	acr-16	NP_505207.1
Caenorhabditis elegans (roundworm)	acr-25	NP_001023570.2
Caenorhabditis elegans (roundworm)	acr-7	NP_495647.1
Pan troglodytes (chimpanzee)	CHRNA7	XP_001164138.1
	CHRNA7	NP_001028055.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008588	XP_314691.2
Caenorhabditis elegans (roundworm)	acr-11	NP_491906.1
Caenorhabditis elegans (roundworm)	acr-9	NP_510285.2
Caenorhabditis elegans (roundworm)	acr-14	NP_495716.1
Homo sapiens (human)	CHRNA7	NP_000737.1
Bos taurus (cattle)	CHRNA7	NP_776940.1
Gallus gallus (chicken)	CHRNA7	NP_989512.2
Caenorhabditis elegans (roundworm)	acr-10	NP_508692.2
Canis lupus familiaris (dog)	CHRNA7	XP_545813.4
Danio rerio (zebrafish)	chrna7	NP_957513.1
Xenopus tropicalis (tropical clawed frog)	LOC100493611	XP_002937717.2

Gene Ontology
Bibliography

Related articles in PubMed

Mutations of cytosolic loop residues impair assembly and maturation of alpha7 nicotinic acetylcholine receptors.
Mukherjee J, Kuryatov A, Moss SJ, Lindstrom JM, Anand R
Journal of neurochemistry110(6)1885-94(2009 Sep)

EphB receptors co-distribute with a nicotinic receptor subtype and regulate nicotinic downstream signaling in neurons.
Liu Z, Conroy WG, Stawicki TM, Nai Q, Neff RA, Berg DK
Molecular and cellular neurosciences38(2)236-44(2008 Jun)

Reversible inhibition of GABAA receptors by alpha7-containing nicotinic receptors on the vertebrate postsynaptic neurons.
Zhang J, Berg DK
The Journal of physiology579(Pt 3)753-63(2007 Mar)

Sequential interplay of nicotinic and GABAergic signaling guides neuronal development.
Liu Z, Neff RA, Berg DK
Science (New York, N.Y.)314(5805)1610-3(2006 Dec)

Rapid activity-driven SNARE-dependent trafficking of nicotinic receptors on somatic spines.
Liu Z, Tearle AW, Nai Q, Berg DK
The Journal of neuroscience : the official journal of the Society for Neuroscience25(5)1159-68(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findinfgs show efficient assembly of alpha7 AChRs is influenced by several regions of the large cytoplasmic domain, as well perhaps by other parts of its structure, and requires as yet unknown factors not required by other AChR subtypes
Title: Mutations of cytosolic loop residues impair assembly and maturation of alpha7 nicotinic acetylcholine receptors.

EphB2 receptors are co-localized with postsynaptic alpha7-nAChRs on chick ciliary ganglion neurons and that treatment of the cells with an ephrinB1 construct to activate the EphB receptors exerts physical restraints on both classes of receptors
Title: EphB receptors co-distribute with a nicotinic receptor subtype and regulate nicotinic downstream signaling in neurons.

regulation of GABAA receptors by alpha7-nAChRs on the postsynaptic neuron and identify a new mechanism by which nicotinic cholinergic signalling influences nervous system function
Title: Reversible inhibition of GABAA receptors by alpha7-containing nicotinic receptors on the vertebrate postsynaptic neurons.

findings show that spontaneous nicotinic cholinergic activity is responsible for terminating GABAergic excitation and
Title: Sequential interplay of nicotinic and GABAergic signaling guides neuronal development.

SNARE-dependent trafficking appears to be essential for maintaining functional coupling between alpha7-receptor responses and downstream signaling.
Title: Rapid activity-driven SNARE-dependent trafficking of nicotinic receptors on somatic spines.

The following CHRNA7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRNA7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa28211	NM_204181.2 Latest version!	Gallus gallus cholinergic receptor nicotinic alpha 7 subunit (CHRNA7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRNA7 ( NM_204181.2 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989512.2 Gallus gallus cholinergic receptor nicotinic alpha 7 subunit (CHRNA7), mRNA.

Price & Availability↑

CloneID	OGa28211
Clone ID Related Accession (Same CDS sequence)	NM_204181.2
Accession Version	NM_204181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Gallus gallus(chicken)
Product	neuronal acetylcholine receptor subunit alpha-7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000047.1. On Jan 19, 2011 this sequence version replaced NM_204181.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52295.1, X68586.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201361 [ECO:0000350] ##Evidence-Data-END##

ATGGGCCTCC AGGCGCTGAT GCTGTGGCTG CTGGCGGCGG CGGGGCTCGT GCGCGAGTCC 
CTGCAAGGAG AGTTCCAAAG GAAGCTGTAC AAGGAGCTGC TGAAGAACTA CAACCCTCTG 
GAACGACCAG TTGCAAATGA CTCCCAGCCG CTCACTGTCT ATTTCACTCT CAGCCTCATG 
CAGATCATGG ATGTGGATGA AAAGAATCAA GTATTAACAA CAAACATCTG GCTACAAATG 
TACTGGACAG ATCATTACTT ACAGTGGAAT GTGTCTGAAT ACCCTGGAGT GAAGAACGTC 
CGTTTTCCTG ATGGACTGAT TTGGAAGCCA GATATTCTTC TCTATAACAG TGCTGATGAA 
AGATTTGATG CTACATTTCA CACTAATGTT TTAGTCAATT CTTCGGGACA CTGCCAATAT 
CTGCCACCAG GCATATTTAA AAGCTCATGC TACATAGACG TGCGTTGGTT TCCATTTGAT 
GTTCAGAAGT GCAATCTGAA GTTTGGATCT TGGACATATG GAGGCTGGTC CTTAGACTTA 
CAAATGCAAG AAGCAGATAT ATCCGGCTAT ATTTCAAATG GAGAGTGGGA TTTAGTAGGA 
ATTCCTGGGA AGAGAACTGA GAGCTTTTAT GAGTGCTGTA AAGAACCATA CCCAGATATC 
ACATTCACAG TAACCATGAG ACGCAGAACT CTCTACTACG GGCTCAACCT TCTTATTCCC 
TGTGTACTGA TATCAGCACT TGCCTTATTA GTCTTTCTGC TTCCAGCAGA CTCAGGAGAA 
AAGATCTCAC TAGGTATAAC AGTTTTATTG TCTCTCACCG TCTTCATGTT ACTCGTGGCT 
GAAATTATGC CAGCAACATC TGATTCTGTG CCCTTAATTG CTCAGTATTT TGCCAGCACC 
ATGATTATTG TTGGCCTCTC TGTTGTTGTC ACTGTTATCG TTCTACAATA CCATCATCAC 
GATCCAGATG GGGGAAAAAT GCCTAAATGG ACAAGAGTCA TCCTTCTGAA TTGGTGTGCT 
TGGTTTCTGA GGATGAAGAG ACCAGGGGAA GATAAAGTGC GTCCCGCCTG TCAACATAAA 
CAGCGTCGAT GCAGCCTGTC AAGCATGGAG ATGAACACTG TGAGTGGTCA GCAATGCAGT 
AATGGGAACA TGCTGTATAT TGGGTTTCGA GGGCTGGATG GGGTTCACTG CACACCCACC 
ACTGATTCAG GGGTGATCTG TGGGAGGATG ACCTGTTCAC CAACAGAGGA AGAAAATCTT 
CTGCACAGTG GCCACCCCTC TGAAGGCGAC CCAGATTTGG CTAAGATCCT GGAAGAGGTC 
AGATACATTG CAAACAGGTT CAGAGACCAG GATGAAGAAG AAGCCATTTG CAACGAATGG 
AAGTTTGCAG CCTCTGTAGT AGATCGGCTC TGCTTGATGG CATTTTCGGT CTTCACCATC 
ATTTGTACAA TTGGCATCTT AATGTCAGCA CCAAACTTTG TAGAGGCTGT CTCTAAAGAT 
TTTGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989512.2
CDS	111..1619
Translation	MGLQALMLWLLAAAGLVRESLQGEFQRKLYKELLKNYNPLERPVANDSQPLTVYFTLSLMQIMDVDEKNQVLTTNIWLQMYWTDHYLQWNVSEYPGVKNVRFPDGLIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQKCNLKFGSWTYGGWSLDLQMQEADISGYISNGEWDLVGIPGKRTESFYECCKEPYPDITFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLSSMEMNTVSGQQCSNGNMLYIGFRGLDGVHCTPTTDSGVICGRMTCSPTEEENLLHSGHPSEGDPDLAKILEEVRYIANRFRDQDEEEAICNEWKFAASVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA

Target ORF information:

RefSeq Version	NM_204181.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus cholinergic receptor nicotinic alpha 7 subunit (CHRNA7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204181.2

ORF Insert Sequence:

ATGGGCCTCC AGGCGCTGAT GCTGTGGCTG CTGGCGGCGG CGGGGCTCGT GCGCGAGTCC 
CTGCAAGGAG AGTTCCAAAG GAAGCTGTAC AAGGAGCTGC TGAAGAACTA CAACCCTCTG 
GAACGACCAG TTGCAAATGA CTCCCAGCCG CTCACTGTCT ATTTCACTCT CAGCCTCATG 
CAGATCATGG ATGTGGATGA AAAGAATCAA GTATTAACAA CAAACATCTG GCTACAAATG 
TACTGGACAG ATCATTACTT ACAGTGGAAT GTGTCTGAAT ACCCTGGAGT GAAGAACGTC 
CGTTTTCCTG ATGGACTGAT TTGGAAGCCA GATATTCTTC TCTATAACAG TGCTGATGAA 
AGATTTGATG CTACATTTCA CACTAATGTT TTAGTCAATT CTTCGGGACA CTGCCAATAT 
CTGCCACCAG GCATATTTAA AAGCTCATGC TACATAGACG TGCGTTGGTT TCCATTTGAT 
GTTCAGAAGT GCAATCTGAA GTTTGGATCT TGGACATATG GAGGCTGGTC CTTAGACTTA 
CAAATGCAAG AAGCAGATAT ATCCGGCTAT ATTTCAAATG GAGAGTGGGA TTTAGTAGGA 
ATTCCTGGGA AGAGAACTGA GAGCTTTTAT GAGTGCTGTA AAGAACCATA CCCAGATATC 
ACATTCACAG TAACCATGAG ACGCAGAACT CTCTACTACG GGCTCAACCT TCTTATTCCC 
TGTGTACTGA TATCAGCACT TGCCTTATTA GTCTTTCTGC TTCCAGCAGA CTCAGGAGAA 
AAGATCTCAC TAGGTATAAC AGTTTTATTG TCTCTCACCG TCTTCATGTT ACTCGTGGCT 
GAAATTATGC CAGCAACATC TGATTCTGTG CCCTTAATTG CTCAGTATTT TGCCAGCACC 
ATGATTATTG TTGGCCTCTC TGTTGTTGTC ACTGTTATCG TTCTACAATA CCATCATCAC 
GATCCAGATG GGGGAAAAAT GCCTAAATGG ACAAGAGTCA TCCTTCTGAA TTGGTGTGCT 
TGGTTTCTGA GGATGAAGAG ACCAGGGGAA GATAAAGTGC GTCCCGCCTG TCAACATAAA 
CAGCGTCGAT GCAGCCTGTC AAGCATGGAG ATGAACACTG TGAGTGGTCA GCAATGCAGT 
AATGGGAACA TGCTGTATAT TGGGTTTCGA GGGCTGGATG GGGTTCACTG CACACCCACC 
ACTGATTCAG GGGTGATCTG TGGGAGGATG ACCTGTTCAC CAACAGAGGA AGAAAATCTT 
CTGCACAGTG GCCACCCCTC TGAAGGCGAC CCAGATTTGG CTAAGATCCT GGAAGAGGTC 
AGATACATTG CAAACAGGTT CAGAGACCAG GATGAAGAAG AAGCCATTTG CAACGAATGG 
AAGTTTGCAG CCTCTGTAGT AGATCGGCTC TGCTTGATGG CATTTTCGGT CTTCACCATC 
ATTTGTACAA TTGGCATCTT AATGTCAGCA CCAAACTTTG TAGAGGCTGT CTCTAAAGAT 
TTTGCTTAA