DGKE cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKE
Entrez Gene ID	103116742
Full Name	diacylglycerol kinase epsilon
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007526694.2	XP_007526756.1	diacylglycerol kinase epsilon

Pathways from BioSystems
Gene Ontology

The following DGKE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr14740	XM_007526694.2 Latest version!	Erinaceus europaeus diacylglycerol kinase epsilon (DGKE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKE ( XM_007526694.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007526756.1 Erinaceus europaeus diacylglycerol kinase epsilon (DGKE), mRNA.

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CloneID	OEr14740
Clone ID Related Accession (Same CDS sequence)	XM_007526694.2
Accession Version	XM_007526694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	diacylglycerol kinase epsilon
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804231.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007526694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGGG AGCAGCGGCC GGCGCCCTAC CGGGGCCTGT TTGCGGACGG GCACCTGGTG 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGCTCATCA CCTGCTGGTG CAGCTTCCAG 
CGGTCGCGCC GCCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGGTGGCGC 
GACACGGACC TGTTCAGCCA GCCCACCTAC TGCTGCGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG CGGCCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC AGTGCAAGGA GATCATGCTC AAGAGTGACG GCAGGGCCCC GAGCCCCATG 
CCCCACCACT GGATCCGGGG CAACGTCCCC CTGTGCAGCT ACTGTGCGGT TTGCAAGCAG 
CAGTGCGGCA GTCAGCCCAA GCTCTGTGAT TACAGGTGCA TTTGGTGTCA GAAAACAGTT 
CATGATGAGT GCATGAAAAA TGGCTTAAGG AGTGAAAAGT GCAATTTTGG AGAATTCAAA 
AATCTCATCA TTCCACCAAG TTATCTGACG TCTATTAACC AGATGCGTAA AGACAGAAAA 
ACAGATTATG CAATGCTAGC CTCAACACTT GGAAAGAATT GGACTCCATT AATAGTCCTG 
GCCAACTCTC GTAGTGGTAC TAACATGGGA GAAGGACTGT TGGGAGAATT TAGGATCCTC 
CTAAATCCAG TTCAGGTTTT TGATGTAACA AAAACTCCCC CTGCTAAAGC CTTGCAACTT 
TGTACTCTGC TTCCCTACTG CTCAGCTCGA GTACTTGTTT GTGGAGGGGA CGGTACTGTG 
GGCTGGGTCC TGGATGCAGT GGATGAAATG AAATTTAAGG GACAGGAAAA ATACATTCCA 
CAGGTTGCTG TCCTGCCTTT GGGAACAGGC AATGATCTGT CCAATACCTT GGGTTGGGGT 
GCAGGTTATG CTGGAGAAAT CCCAGTTGCA CAGGTCTTAC GAAATGTAAT GGAAGCAGAT 
GGAATTACAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA TAACTTAAGA 
AAGCCCAAGG AATTCACAAT GAACAACTAT TTTTCTGTTG GACCTGATGC TCTCATGGCT 
CTCAATTTCC ATGCTCATCG TGAGAAGGCG CCATCTCTGT TTTCTAACAG AATTCTTAAT 
AAGGCTGTTT ACCTATTCTA TGGAACCAAA GATTGTTTAG TACAAGAATG TAAAGATTTG 
AATAAAAAAG TCGAGCTAGA ACTGGATGGT GAGCGAGTGG AACTGCCTAA TTTGGAAGGC 
ATCATAGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GATGAAACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAC TAGAAGTTGT TGGAGTATAT 
GGATCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCAA ATCCTTATCG AATAGGACAA 
GCACATACAG TGAGGCTGAT TTTGAAGTGC TCAATGATGC CAATGCAGGT AGATGGGGAG 
CCATGGGCCC AAGGGCCTTG CACTGTCACC ATAACTCACA AGACACATGC ATTGATGTTA 
TATTTCTCTG GGGAACAAAC GGATGATGAC GTCTCTAGTA CTTCGGATCA AGAGGACTTG 
AAGGAAGCTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007526756.1
CDS	36..1730
Translation	MDGEQRPAPYRGLFADGHLVLWTLCSVLLPVLITCWCSFQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLKKADKRFQCKEIMLKSDGRAPSPMPHHWIRGNVPLCSYCAVCKQQCGSQPKLCDYRCIWCQKTVHDECMKNGLRSEKCNFGEFKNLIIPPSYLTSINQMRKDRKTDYAMLASTLGKNWTPLIVLANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPAKALQLCTLLPYCSARVLVCGGDGTVGWVLDAVDEMKFKGQEKYIPQVAVLPLGTGNDLSNTLGWGAGYAGEIPVAQVLRNVMEADGITLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSNRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVELPNLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPYRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHALMLYFSGEQTDDDVSSTSDQEDLKEAE

Target ORF information:

RefSeq Version	XM_007526694.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus diacylglycerol kinase epsilon (DGKE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007526694.2

ORF Insert Sequence:

ATGGACGGGG AGCAGCGGCC GGCGCCCTAC CGGGGCCTGT TTGCGGACGG GCACCTGGTG 
CTGTGGACGC TGTGCTCGGT GCTGCTGCCC GTGCTCATCA CCTGCTGGTG CAGCTTCCAG 
CGGTCGCGCC GCCAGCTGCA CCGCAGGGAC ATCTTCCGCA AGAGCAAGCA CGGGTGGCGC 
GACACGGACC TGTTCAGCCA GCCCACCTAC TGCTGCGTGT GCGCGCAGCA CATCCTGCAG 
GGCGCCTTCT GCGACTGCTG CGGCCTGCGC GTGGACGAGG GCTGCCTCAA GAAGGCCGAC 
AAGCGCTTCC AGTGCAAGGA GATCATGCTC AAGAGTGACG GCAGGGCCCC GAGCCCCATG 
CCCCACCACT GGATCCGGGG CAACGTCCCC CTGTGCAGCT ACTGTGCGGT TTGCAAGCAG 
CAGTGCGGCA GTCAGCCCAA GCTCTGTGAT TACAGGTGCA TTTGGTGTCA GAAAACAGTT 
CATGATGAGT GCATGAAAAA TGGCTTAAGG AGTGAAAAGT GCAATTTTGG AGAATTCAAA 
AATCTCATCA TTCCACCAAG TTATCTGACG TCTATTAACC AGATGCGTAA AGACAGAAAA 
ACAGATTATG CAATGCTAGC CTCAACACTT GGAAAGAATT GGACTCCATT AATAGTCCTG 
GCCAACTCTC GTAGTGGTAC TAACATGGGA GAAGGACTGT TGGGAGAATT TAGGATCCTC 
CTAAATCCAG TTCAGGTTTT TGATGTAACA AAAACTCCCC CTGCTAAAGC CTTGCAACTT 
TGTACTCTGC TTCCCTACTG CTCAGCTCGA GTACTTGTTT GTGGAGGGGA CGGTACTGTG 
GGCTGGGTCC TGGATGCAGT GGATGAAATG AAATTTAAGG GACAGGAAAA ATACATTCCA 
CAGGTTGCTG TCCTGCCTTT GGGAACAGGC AATGATCTGT CCAATACCTT GGGTTGGGGT 
GCAGGTTATG CTGGAGAAAT CCCAGTTGCA CAGGTCTTAC GAAATGTAAT GGAAGCAGAT 
GGAATTACAC TAGATAGATG GAAAGTTCAA GTAACAAATA AAGGATACTA TAACTTAAGA 
AAGCCCAAGG AATTCACAAT GAACAACTAT TTTTCTGTTG GACCTGATGC TCTCATGGCT 
CTCAATTTCC ATGCTCATCG TGAGAAGGCG CCATCTCTGT TTTCTAACAG AATTCTTAAT 
AAGGCTGTTT ACCTATTCTA TGGAACCAAA GATTGTTTAG TACAAGAATG TAAAGATTTG 
AATAAAAAAG TCGAGCTAGA ACTGGATGGT GAGCGAGTGG AACTGCCTAA TTTGGAAGGC 
ATCATAGTTC TGAACATTGG ATACTGGGGC GGTGGCTGCA GACTGTGGGA AGGGATGGGA 
GATGAAACTT ACCCTCTAGC CAGGCATGAT GATGGTTTAC TAGAAGTTGT TGGAGTATAT 
GGATCTTTCC ACTGTGCTCA GATTCAAGTG AAACTGGCAA ATCCTTATCG AATAGGACAA 
GCACATACAG TGAGGCTGAT TTTGAAGTGC TCAATGATGC CAATGCAGGT AGATGGGGAG 
CCATGGGCCC AAGGGCCTTG CACTGTCACC ATAACTCACA AGACACATGC ATTGATGTTA 
TATTTCTCTG GGGAACAAAC GGATGATGAC GTCTCTAGTA CTTCGGATCA AGAGGACTTG 
AAGGAAGCTG AGTAG