HYOU1 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HYOU1
Entrez Gene ID	103113978
Full Name	hypoxia up-regulated 1
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016188377.1	XP_016043863.1	hypoxia up-regulated protein 1 isoform X1
XM_007523593.2	XP_007523655.1	hypoxia up-regulated protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following HYOU1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HYOU1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr11499	XM_007523593.2 Latest version!	Erinaceus europaeus hypoxia up-regulated 1 (HYOU1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OEr11498	XM_016188377.1 Latest version!	Erinaceus europaeus hypoxia up-regulated 1 (HYOU1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HYOU1 ( XM_007523593.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007523655.1 Erinaceus europaeus hypoxia up-regulated 1 (HYOU1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OEr11499
Clone ID Related Accession (Same CDS sequence)	XM_007523593.2
Accession Version	XM_007523593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	hypoxia up-regulated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804130.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007523593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTACCA CAGTCAGAAG ACAGAGACAG AGAAGACTAC CCTGTTGGGT CTTGATGGTT 
GTGCTCTTGG CAGACCTGTT GGCACTGAGC GATACACTGG CAGTGATGTC TGTAGATCTA 
GGCAGTGAGT CCATGAAGGT GGCGATCGTC AAACCTGGAG TGCCCATGGA AATTGTCTTG 
AACAAGGAAT CCCGGAGGAA AACCCCAGTG ACTGTGACCC TAAAAGAAAA TGAAAGGTTC 
TTTGGAGACA GTGCAGCAAG CATGGCTATC AAGAATCCAA AGGCTACACT TCGTTACTTC 
CAGCACCTCC TGGGGAAGCA GGAGAACAAT CCCCATGTGG CCCTTTATAG AGCTCGTTTT 
CCAGAGCATG AGCTAGCCTT TGACCCACAG AGGCAGACTG TGCGCTTCCA AATCAGCCCG 
CAGCTGCACT TCTCTCCTGA GGAAGTACTG GGCATGGTTC TCAATTACTC CCGTTCCCTG 
GCTGAGGATT TTGCAGAGCA GCCCATCAAG GATGCCGTGA TCACTGTGCC AGCCTTCTTC 
AATCAGGCTG AGCGCCGGGC CGTGCTGCAG GCTGCTCGCA TGGCAGGTCT CAAAGTGCTA 
CAGCTTATCA ATGACAACAC TGCTACTGCC CTCAGCTATG GGGTCTTCCG CCGGAAGGAT 
ATCAACACCA CTGCCCAGAA TGTCATGTTT TACGACATGG GCTCAGGCAG CACTGTGTGC 
ACCATCGTGA CCTACCAGAC AGTGAAGACT AAAGAAGCAG GGATGCAGCC CCAGTTGCAA 
ATCCGGGGAG TGGGCTTTGA CCGCACCCTG GGAGGCCTGG AGATGGAGCT CAGGCTTCGG 
GAGCACCTGG CCAAGCTTTT CAATGAGCAG CGCAAGGGCC AGAGTACCAA GGATGTGCGG 
GAGAACCCCC GGGCCATGGC TAAACTGCTG CGGGAGGCTA ATCGCCTCAA GACTGTCCTG 
AGTGCCAATG CTGATCACAT GGCACAGATT GAAGGCCTCA TGGACGATGT GGACTTTAAG 
GCCAAAGTGA CTCGAGCAGA GTTTGAGGAA CTGTGTGCTG ACCTGTTTGA GCGAGTGCCT 
GGTCCTGTGC AGCAGGCCCT GCAGAGTGCT GAAATGAGTC TGGATGAGAT TGAACAGGTG 
ATCCTGGTGG GCGGGGCCAC TCGGGTCCCC AGAGTTCAAG AGGTGCTGCT GAAGGCCGTG 
GGCAAGGAGG AGCTCGGCAA GAACATCAAC GCAGATGAAG CAGCTGCCAT GGGTGCTGTA 
TACCAGGCAG CCGCACTCAG CAAGGCCTTC AAAGTGAAGC CTTTTGTAGT CCGAGATGCG 
GTTATCTACC CCATCCTGGT GGAATTCATC AGGGAGGTAG AGGAGGACCC CGGGGTACGC 
AACCTGAAGC ACAATAAGCG TGTCCTCTTC TCCCGGATGG GGCCTTACCC TCAACGCAAA 
GTCATCACCT TTAACCGCTA TAGCCATGAC TTCAACTTCC ACATCAACTA CGGTGACCTG 
GGCTTCCTGG GGCCTGAGGA CCTTCGGGTA TTTGGCTCCC AGAATCTGAC CACAGTGAAC 
CTAAAAGGTG TGGGCGAGAG CTTCAAGAAG TATCCCAACT ATGAGTCCAA GGGCATCAAG 
GCTCATTTCA ACTTGGATGA GAGTGGTGTG CTCAGCCTGG ACCGGGTGGA ATCTGTTTTT 
GAGACACTGG TGGAGGACAG CCCAGAAGAG GAATCTACTC TGACCAAACT TGGCAACACC 
ATCTCCAGTC TGTTTGGAGG AGGGACCACA CCAGATTCTA AAGAGAATGG TACAGATACT 
GTACAGGAGG AAGAGGAGAG TCCTGCAGAG GGGGCCAAAG ATGAAACTGG AGAGCAGGCA 
GAGCTGCGGG AGGAATCTGA GACCCCATCG GAAGATAGCT CTCTGCCTCC ACCCCCTGAG 
CCAAAGGTGG ATGAAGCTCC TCAGGGAGAA AAGGCTGAAG CAAAGGAAAG TGGGGAGAAG 
TCTGAGGCAC AGAAGCCAAG TGAAAAAGGA GAGACAGGAG CTGAGAGTGC CCCCTTGACC 
CCAGAGGAAG CAAAGAAGCA GAAGCCTGCC CGGAAGCAGA GAATGGTGGA GGAGATTGGT 
GTGGAGATGG TTGTTCTAGA CCTGCCTGAC CTGCCGGAGG ATGAGCTGGC CCATTCGGCA 
CAGAGACTTC AGGACTTGAC TATCCGAGAT CTAGAGAAGC AAGAACGTGA GAAAGCTGCT 
AACAGCTTGG AAGCCTTCAT CTTTGAGACC CAGGACAAGC TGTACCAGTC TGAGTACCAG 
GAAGTGTCCA CTGAAGCGCA GAGGGAAGAA ATCTCTGGGA AACTTAGCTC TGCTTCCACC 
TGGCTAGAGG ATGAGGGCTT CGACGCCACC ACAGTGATGT TGAAGGAGAA GCTGGCAGAG 
CTGAGGAAGC TGTGCCAAGG GTTATTTTTT CGGGTGGAAG AACGCAAGAA ATGGCCTGAA 
AGACTATCTG CTCTGGATAA TCTCCTCAAT CATTCCAGCA TGTTCCTCAA GGGTGCCCGG 
CTCATCCCAG AGATGGACCA GATCTTCACT GAAGTGGAAA TGACAACATT AGAGAAAATC 
ATCAATGAGA CCTGGGCCTG GAAGAATGCA ACCGTAGCGG AGCAGTCCAA GCTACCTGCC 
ACAGAGAAGC CTGTGCTGCT CTCAAAGGAC ATTGAGGCCA AGATGATGGC CCTGGACCGT 
GAAGTACAGT ATCTGCTTAA TAAGGCCAAG TTCGCCAAAC CCCGGCCCCG GCCCAAGGAC 
AAGAATGGGA CCCGGACAGA GCCTCCCGTC AATGCCAGTG ACCAGGGGGA GAAGGTCATC 
CCTCCAACAG GCCAGACTGA AGATGCAAAG CCCATTTTGG AACCCGAGAA AGAGTCTGAA 
TCTGAAGTAG CAGATGCTGA ACCTCTGGAG TTAGGAGGCC CTGGAGTAGA AACTGAACAG 
AAGGAGCAAC CAACAGGACA GAAGAAAACC TTGAAGAATG ATGAACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007523655.1
CDS	134..3124
Translation	MATTVRRQRQRRLPCWVLMVVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVTVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQENNPHVALYRARFPEHELAFDPQRQTVRFQISPQLHFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPAFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRRKDINTTAQNVMFYDMGSGSTVCTIVTYQTVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLREHLAKLFNEQRKGQSTKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRAEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVIYPILVEFIREVEEDPGVRNLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDLRVFGSQNLTTVNLKGVGESFKKYPNYESKGIKAHFNLDESGVLSLDRVESVFETLVEDSPEEESTLTKLGNTISSLFGGGTTPDSKENGTDTVQEEEESPAEGAKDETGEQAELREESETPSEDSSLPPPPEPKVDEAPQGEKAEAKESGEKSEAQKPSEKGETGAESAPLTPEEAKKQKPARKQRMVEEIGVEMVVLDLPDLPEDELAHSAQRLQDLTIRDLEKQEREKAANSLEAFIFETQDKLYQSEYQEVSTEAQREEISGKLSSASTWLEDEGFDATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQIFTEVEMTTLEKIINETWAWKNATVAEQSKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFAKPRPRPKDKNGTRTEPPVNASDQGEKVIPPTGQTEDAKPILEPEKESESEVADAEPLELGGPGVETEQKEQPTGQKKTLKNDEL

Target ORF information:

RefSeq Version	XM_007523593.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus hypoxia up-regulated 1 (HYOU1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007523593.2

ORF Insert Sequence:

ATGGCTACCA CAGTCAGAAG ACAGAGACAG AGAAGACTAC CCTGTTGGGT CTTGATGGTT 
GTGCTCTTGG CAGACCTGTT GGCACTGAGC GATACACTGG CAGTGATGTC TGTAGATCTA 
GGCAGTGAGT CCATGAAGGT GGCGATCGTC AAACCTGGAG TGCCCATGGA AATTGTCTTG 
AACAAGGAAT CCCGGAGGAA AACCCCAGTG ACTGTGACCC TAAAAGAAAA TGAAAGGTTC 
TTTGGAGACA GTGCAGCAAG CATGGCTATC AAGAATCCAA AGGCTACACT TCGTTACTTC 
CAGCACCTCC TGGGGAAGCA GGAGAACAAT CCCCATGTGG CCCTTTATAG AGCTCGTTTT 
CCAGAGCATG AGCTAGCCTT TGACCCACAG AGGCAGACTG TGCGCTTCCA AATCAGCCCG 
CAGCTGCACT TCTCTCCTGA GGAAGTACTG GGCATGGTTC TCAATTACTC CCGTTCCCTG 
GCTGAGGATT TTGCAGAGCA GCCCATCAAG GATGCCGTGA TCACTGTGCC AGCCTTCTTC 
AATCAGGCTG AGCGCCGGGC CGTGCTGCAG GCTGCTCGCA TGGCAGGTCT CAAAGTGCTA 
CAGCTTATCA ATGACAACAC TGCTACTGCC CTCAGCTATG GGGTCTTCCG CCGGAAGGAT 
ATCAACACCA CTGCCCAGAA TGTCATGTTT TACGACATGG GCTCAGGCAG CACTGTGTGC 
ACCATCGTGA CCTACCAGAC AGTGAAGACT AAAGAAGCAG GGATGCAGCC CCAGTTGCAA 
ATCCGGGGAG TGGGCTTTGA CCGCACCCTG GGAGGCCTGG AGATGGAGCT CAGGCTTCGG 
GAGCACCTGG CCAAGCTTTT CAATGAGCAG CGCAAGGGCC AGAGTACCAA GGATGTGCGG 
GAGAACCCCC GGGCCATGGC TAAACTGCTG CGGGAGGCTA ATCGCCTCAA GACTGTCCTG 
AGTGCCAATG CTGATCACAT GGCACAGATT GAAGGCCTCA TGGACGATGT GGACTTTAAG 
GCCAAAGTGA CTCGAGCAGA GTTTGAGGAA CTGTGTGCTG ACCTGTTTGA GCGAGTGCCT 
GGTCCTGTGC AGCAGGCCCT GCAGAGTGCT GAAATGAGTC TGGATGAGAT TGAACAGGTG 
ATCCTGGTGG GCGGGGCCAC TCGGGTCCCC AGAGTTCAAG AGGTGCTGCT GAAGGCCGTG 
GGCAAGGAGG AGCTCGGCAA GAACATCAAC GCAGATGAAG CAGCTGCCAT GGGTGCTGTA 
TACCAGGCAG CCGCACTCAG CAAGGCCTTC AAAGTGAAGC CTTTTGTAGT CCGAGATGCG 
GTTATCTACC CCATCCTGGT GGAATTCATC AGGGAGGTAG AGGAGGACCC CGGGGTACGC 
AACCTGAAGC ACAATAAGCG TGTCCTCTTC TCCCGGATGG GGCCTTACCC TCAACGCAAA 
GTCATCACCT TTAACCGCTA TAGCCATGAC TTCAACTTCC ACATCAACTA CGGTGACCTG 
GGCTTCCTGG GGCCTGAGGA CCTTCGGGTA TTTGGCTCCC AGAATCTGAC CACAGTGAAC 
CTAAAAGGTG TGGGCGAGAG CTTCAAGAAG TATCCCAACT ATGAGTCCAA GGGCATCAAG 
GCTCATTTCA ACTTGGATGA GAGTGGTGTG CTCAGCCTGG ACCGGGTGGA ATCTGTTTTT 
GAGACACTGG TGGAGGACAG CCCAGAAGAG GAATCTACTC TGACCAAACT TGGCAACACC 
ATCTCCAGTC TGTTTGGAGG AGGGACCACA CCAGATTCTA AAGAGAATGG TACAGATACT 
GTACAGGAGG AAGAGGAGAG TCCTGCAGAG GGGGCCAAAG ATGAAACTGG AGAGCAGGCA 
GAGCTGCGGG AGGAATCTGA GACCCCATCG GAAGATAGCT CTCTGCCTCC ACCCCCTGAG 
CCAAAGGTGG ATGAAGCTCC TCAGGGAGAA AAGGCTGAAG CAAAGGAAAG TGGGGAGAAG 
TCTGAGGCAC AGAAGCCAAG TGAAAAAGGA GAGACAGGAG CTGAGAGTGC CCCCTTGACC 
CCAGAGGAAG CAAAGAAGCA GAAGCCTGCC CGGAAGCAGA GAATGGTGGA GGAGATTGGT 
GTGGAGATGG TTGTTCTAGA CCTGCCTGAC CTGCCGGAGG ATGAGCTGGC CCATTCGGCA 
CAGAGACTTC AGGACTTGAC TATCCGAGAT CTAGAGAAGC AAGAACGTGA GAAAGCTGCT 
AACAGCTTGG AAGCCTTCAT CTTTGAGACC CAGGACAAGC TGTACCAGTC TGAGTACCAG 
GAAGTGTCCA CTGAAGCGCA GAGGGAAGAA ATCTCTGGGA AACTTAGCTC TGCTTCCACC 
TGGCTAGAGG ATGAGGGCTT CGACGCCACC ACAGTGATGT TGAAGGAGAA GCTGGCAGAG 
CTGAGGAAGC TGTGCCAAGG GTTATTTTTT CGGGTGGAAG AACGCAAGAA ATGGCCTGAA 
AGACTATCTG CTCTGGATAA TCTCCTCAAT CATTCCAGCA TGTTCCTCAA GGGTGCCCGG 
CTCATCCCAG AGATGGACCA GATCTTCACT GAAGTGGAAA TGACAACATT AGAGAAAATC 
ATCAATGAGA CCTGGGCCTG GAAGAATGCA ACCGTAGCGG AGCAGTCCAA GCTACCTGCC 
ACAGAGAAGC CTGTGCTGCT CTCAAAGGAC ATTGAGGCCA AGATGATGGC CCTGGACCGT 
GAAGTACAGT ATCTGCTTAA TAAGGCCAAG TTCGCCAAAC CCCGGCCCCG GCCCAAGGAC 
AAGAATGGGA CCCGGACAGA GCCTCCCGTC AATGCCAGTG ACCAGGGGGA GAAGGTCATC 
CCTCCAACAG GCCAGACTGA AGATGCAAAG CCCATTTTGG AACCCGAGAA AGAGTCTGAA 
TCTGAAGTAG CAGATGCTGA ACCTCTGGAG TTAGGAGGCC CTGGAGTAGA AACTGAACAG 
AAGGAGCAAC CAACAGGACA GAAGAAAACC TTGAAGAATG ATGAACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HYOU1 ( XM_016188377.1 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_016043863.1 Erinaceus europaeus hypoxia up-regulated 1 (HYOU1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OEr11498
Clone ID Related Accession (Same CDS sequence)	XM_016188377.1
Accession Version	XM_016188377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	hypoxia up-regulated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804130.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAA GGAGCACAAT GGCTACCACA GTCAGAAGAC AGAGACAGAG AAGACTACCC 
TGTTGGGTCT TGATGGTTGT GCTCTTGGCA GACCTGTTGG CACTGAGCGA TACACTGGCA 
GTGATGTCTG TAGATCTAGG CAGTGAGTCC ATGAAGGTGG CGATCGTCAA ACCTGGAGTG 
CCCATGGAAA TTGTCTTGAA CAAGGAATCC CGGAGGAAAA CCCCAGTGAC TGTGACCCTA 
AAAGAAAATG AAAGGTTCTT TGGAGACAGT GCAGCAAGCA TGGCTATCAA GAATCCAAAG 
GCTACACTTC GTTACTTCCA GCACCTCCTG GGGAAGCAGG AGAACAATCC CCATGTGGCC 
CTTTATAGAG CTCGTTTTCC AGAGCATGAG CTAGCCTTTG ACCCACAGAG GCAGACTGTG 
CGCTTCCAAA TCAGCCCGCA GCTGCACTTC TCTCCTGAGG AAGTACTGGG CATGGTTCTC 
AATTACTCCC GTTCCCTGGC TGAGGATTTT GCAGAGCAGC CCATCAAGGA TGCCGTGATC 
ACTGTGCCAG CCTTCTTCAA TCAGGCTGAG CGCCGGGCCG TGCTGCAGGC TGCTCGCATG 
GCAGGTCTCA AAGTGCTACA GCTTATCAAT GACAACACTG CTACTGCCCT CAGCTATGGG 
GTCTTCCGCC GGAAGGATAT CAACACCACT GCCCAGAATG TCATGTTTTA CGACATGGGC 
TCAGGCAGCA CTGTGTGCAC CATCGTGACC TACCAGACAG TGAAGACTAA AGAAGCAGGG 
ATGCAGCCCC AGTTGCAAAT CCGGGGAGTG GGCTTTGACC GCACCCTGGG AGGCCTGGAG 
ATGGAGCTCA GGCTTCGGGA GCACCTGGCC AAGCTTTTCA ATGAGCAGCG CAAGGGCCAG 
AGTACCAAGG ATGTGCGGGA GAACCCCCGG GCCATGGCTA AACTGCTGCG GGAGGCTAAT 
CGCCTCAAGA CTGTCCTGAG TGCCAATGCT GATCACATGG CACAGATTGA AGGCCTCATG 
GACGATGTGG ACTTTAAGGC CAAAGTGACT CGAGCAGAGT TTGAGGAACT GTGTGCTGAC 
CTGTTTGAGC GAGTGCCTGG TCCTGTGCAG CAGGCCCTGC AGAGTGCTGA AATGAGTCTG 
GATGAGATTG AACAGGTGAT CCTGGTGGGC GGGGCCACTC GGGTCCCCAG AGTTCAAGAG 
GTGCTGCTGA AGGCCGTGGG CAAGGAGGAG CTCGGCAAGA ACATCAACGC AGATGAAGCA 
GCTGCCATGG GTGCTGTATA CCAGGCAGCC GCACTCAGCA AGGCCTTCAA AGTGAAGCCT 
TTTGTAGTCC GAGATGCGGT TATCTACCCC ATCCTGGTGG AATTCATCAG GGAGGTAGAG 
GAGGACCCCG GGGTACGCAA CCTGAAGCAC AATAAGCGTG TCCTCTTCTC CCGGATGGGG 
CCTTACCCTC AACGCAAAGT CATCACCTTT AACCGCTATA GCCATGACTT CAACTTCCAC 
ATCAACTACG GTGACCTGGG CTTCCTGGGG CCTGAGGACC TTCGGGTATT TGGCTCCCAG 
AATCTGACCA CAGTGAACCT AAAAGGTGTG GGCGAGAGCT TCAAGAAGTA TCCCAACTAT 
GAGTCCAAGG GCATCAAGGC TCATTTCAAC TTGGATGAGA GTGGTGTGCT CAGCCTGGAC 
CGGGTGGAAT CTGTTTTTGA GACACTGGTG GAGGACAGCC CAGAAGAGGA ATCTACTCTG 
ACCAAACTTG GCAACACCAT CTCCAGTCTG TTTGGAGGAG GGACCACACC AGATTCTAAA 
GAGAATGGTA CAGATACTGT ACAGGAGGAA GAGGAGAGTC CTGCAGAGGG GGCCAAAGAT 
GAAACTGGAG AGCAGGCAGA GCTGCGGGAG GAATCTGAGA CCCCATCGGA AGATAGCTCT 
CTGCCTCCAC CCCCTGAGCC AAAGGTGGAT GAAGCTCCTC AGGGAGAAAA GGCTGAAGCA 
AAGGAAAGTG GGGAGAAGTC TGAGGCACAG AAGCCAAGTG AAAAAGGAGA GACAGGAGCT 
GAGAGTGCCC CCTTGACCCC AGAGGAAGCA AAGAAGCAGA AGCCTGCCCG GAAGCAGAGA 
ATGGTGGAGG AGATTGGTGT GGAGATGGTT GTTCTAGACC TGCCTGACCT GCCGGAGGAT 
GAGCTGGCCC ATTCGGCACA GAGACTTCAG GACTTGACTA TCCGAGATCT AGAGAAGCAA 
GAACGTGAGA AAGCTGCTAA CAGCTTGGAA GCCTTCATCT TTGAGACCCA GGACAAGCTG 
TACCAGTCTG AGTACCAGGA AGTGTCCACT GAAGCGCAGA GGGAAGAAAT CTCTGGGAAA 
CTTAGCTCTG CTTCCACCTG GCTAGAGGAT GAGGGCTTCG ACGCCACCAC AGTGATGTTG 
AAGGAGAAGC TGGCAGAGCT GAGGAAGCTG TGCCAAGGGT TATTTTTTCG GGTGGAAGAA 
CGCAAGAAAT GGCCTGAAAG ACTATCTGCT CTGGATAATC TCCTCAATCA TTCCAGCATG 
TTCCTCAAGG GTGCCCGGCT CATCCCAGAG ATGGACCAGA TCTTCACTGA AGTGGAAATG 
ACAACATTAG AGAAAATCAT CAATGAGACC TGGGCCTGGA AGAATGCAAC CGTAGCGGAG 
CAGTCCAAGC TACCTGCCAC AGAGAAGCCT GTGCTGCTCT CAAAGGACAT TGAGGCCAAG 
ATGATGGCCC TGGACCGTGA AGTACAGTAT CTGCTTAATA AGGCCAAGTT CGCCAAACCC 
CGGCCCCGGC CCAAGGACAA GAATGGGACC CGGACAGAGC CTCCCGTCAA TGCCAGTGAC 
CAGGGGGAGA AGGTCATCCC TCCAACAGGC CAGACTGAAG ATGCAAAGCC CATTTTGGAA 
CCCGAGAAAG AGTCTGAATC TGAAGTAGCA GATGCTGAAC CTCTGGAGTT AGGAGGCCCT 
GGAGTAGAAA CTGAACAGAA GGAGCAACCA ACAGGACAGA AGAAAACCTT GAAGAATGAT 
GAACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016043863.1
CDS	10..3018
Translation	MNSRSTMATTVRRQRQRRLPCWVLMVVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVTVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQENNPHVALYRARFPEHELAFDPQRQTVRFQISPQLHFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPAFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRRKDINTTAQNVMFYDMGSGSTVCTIVTYQTVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLREHLAKLFNEQRKGQSTKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRAEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVIYPILVEFIREVEEDPGVRNLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDLRVFGSQNLTTVNLKGVGESFKKYPNYESKGIKAHFNLDESGVLSLDRVESVFETLVEDSPEEESTLTKLGNTISSLFGGGTTPDSKENGTDTVQEEEESPAEGAKDETGEQAELREESETPSEDSSLPPPPEPKVDEAPQGEKAEAKESGEKSEAQKPSEKGETGAESAPLTPEEAKKQKPARKQRMVEEIGVEMVVLDLPDLPEDELAHSAQRLQDLTIRDLEKQEREKAANSLEAFIFETQDKLYQSEYQEVSTEAQREEISGKLSSASTWLEDEGFDATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQIFTEVEMTTLEKIINETWAWKNATVAEQSKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFAKPRPRPKDKNGTRTEPPVNASDQGEKVIPPTGQTEDAKPILEPEKESESEVADAEPLELGGPGVETEQKEQPTGQKKTLKNDEL

Target ORF information:

RefSeq Version	XM_016188377.1
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus hypoxia up-regulated 1 (HYOU1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016188377.1

ORF Insert Sequence:

ATGAACTCAA GGAGCACAAT GGCTACCACA GTCAGAAGAC AGAGACAGAG AAGACTACCC 
TGTTGGGTCT TGATGGTTGT GCTCTTGGCA GACCTGTTGG CACTGAGCGA TACACTGGCA 
GTGATGTCTG TAGATCTAGG CAGTGAGTCC ATGAAGGTGG CGATCGTCAA ACCTGGAGTG 
CCCATGGAAA TTGTCTTGAA CAAGGAATCC CGGAGGAAAA CCCCAGTGAC TGTGACCCTA 
AAAGAAAATG AAAGGTTCTT TGGAGACAGT GCAGCAAGCA TGGCTATCAA GAATCCAAAG 
GCTACACTTC GTTACTTCCA GCACCTCCTG GGGAAGCAGG AGAACAATCC CCATGTGGCC 
CTTTATAGAG CTCGTTTTCC AGAGCATGAG CTAGCCTTTG ACCCACAGAG GCAGACTGTG 
CGCTTCCAAA TCAGCCCGCA GCTGCACTTC TCTCCTGAGG AAGTACTGGG CATGGTTCTC 
AATTACTCCC GTTCCCTGGC TGAGGATTTT GCAGAGCAGC CCATCAAGGA TGCCGTGATC 
ACTGTGCCAG CCTTCTTCAA TCAGGCTGAG CGCCGGGCCG TGCTGCAGGC TGCTCGCATG 
GCAGGTCTCA AAGTGCTACA GCTTATCAAT GACAACACTG CTACTGCCCT CAGCTATGGG 
GTCTTCCGCC GGAAGGATAT CAACACCACT GCCCAGAATG TCATGTTTTA CGACATGGGC 
TCAGGCAGCA CTGTGTGCAC CATCGTGACC TACCAGACAG TGAAGACTAA AGAAGCAGGG 
ATGCAGCCCC AGTTGCAAAT CCGGGGAGTG GGCTTTGACC GCACCCTGGG AGGCCTGGAG 
ATGGAGCTCA GGCTTCGGGA GCACCTGGCC AAGCTTTTCA ATGAGCAGCG CAAGGGCCAG 
AGTACCAAGG ATGTGCGGGA GAACCCCCGG GCCATGGCTA AACTGCTGCG GGAGGCTAAT 
CGCCTCAAGA CTGTCCTGAG TGCCAATGCT GATCACATGG CACAGATTGA AGGCCTCATG 
GACGATGTGG ACTTTAAGGC CAAAGTGACT CGAGCAGAGT TTGAGGAACT GTGTGCTGAC 
CTGTTTGAGC GAGTGCCTGG TCCTGTGCAG CAGGCCCTGC AGAGTGCTGA AATGAGTCTG 
GATGAGATTG AACAGGTGAT CCTGGTGGGC GGGGCCACTC GGGTCCCCAG AGTTCAAGAG 
GTGCTGCTGA AGGCCGTGGG CAAGGAGGAG CTCGGCAAGA ACATCAACGC AGATGAAGCA 
GCTGCCATGG GTGCTGTATA CCAGGCAGCC GCACTCAGCA AGGCCTTCAA AGTGAAGCCT 
TTTGTAGTCC GAGATGCGGT TATCTACCCC ATCCTGGTGG AATTCATCAG GGAGGTAGAG 
GAGGACCCCG GGGTACGCAA CCTGAAGCAC AATAAGCGTG TCCTCTTCTC CCGGATGGGG 
CCTTACCCTC AACGCAAAGT CATCACCTTT AACCGCTATA GCCATGACTT CAACTTCCAC 
ATCAACTACG GTGACCTGGG CTTCCTGGGG CCTGAGGACC TTCGGGTATT TGGCTCCCAG 
AATCTGACCA CAGTGAACCT AAAAGGTGTG GGCGAGAGCT TCAAGAAGTA TCCCAACTAT 
GAGTCCAAGG GCATCAAGGC TCATTTCAAC TTGGATGAGA GTGGTGTGCT CAGCCTGGAC 
CGGGTGGAAT CTGTTTTTGA GACACTGGTG GAGGACAGCC CAGAAGAGGA ATCTACTCTG 
ACCAAACTTG GCAACACCAT CTCCAGTCTG TTTGGAGGAG GGACCACACC AGATTCTAAA 
GAGAATGGTA CAGATACTGT ACAGGAGGAA GAGGAGAGTC CTGCAGAGGG GGCCAAAGAT 
GAAACTGGAG AGCAGGCAGA GCTGCGGGAG GAATCTGAGA CCCCATCGGA AGATAGCTCT 
CTGCCTCCAC CCCCTGAGCC AAAGGTGGAT GAAGCTCCTC AGGGAGAAAA GGCTGAAGCA 
AAGGAAAGTG GGGAGAAGTC TGAGGCACAG AAGCCAAGTG AAAAAGGAGA GACAGGAGCT 
GAGAGTGCCC CCTTGACCCC AGAGGAAGCA AAGAAGCAGA AGCCTGCCCG GAAGCAGAGA 
ATGGTGGAGG AGATTGGTGT GGAGATGGTT GTTCTAGACC TGCCTGACCT GCCGGAGGAT 
GAGCTGGCCC ATTCGGCACA GAGACTTCAG GACTTGACTA TCCGAGATCT AGAGAAGCAA 
GAACGTGAGA AAGCTGCTAA CAGCTTGGAA GCCTTCATCT TTGAGACCCA GGACAAGCTG 
TACCAGTCTG AGTACCAGGA AGTGTCCACT GAAGCGCAGA GGGAAGAAAT CTCTGGGAAA 
CTTAGCTCTG CTTCCACCTG GCTAGAGGAT GAGGGCTTCG ACGCCACCAC AGTGATGTTG 
AAGGAGAAGC TGGCAGAGCT GAGGAAGCTG TGCCAAGGGT TATTTTTTCG GGTGGAAGAA 
CGCAAGAAAT GGCCTGAAAG ACTATCTGCT CTGGATAATC TCCTCAATCA TTCCAGCATG 
TTCCTCAAGG GTGCCCGGCT CATCCCAGAG ATGGACCAGA TCTTCACTGA AGTGGAAATG 
ACAACATTAG AGAAAATCAT CAATGAGACC TGGGCCTGGA AGAATGCAAC CGTAGCGGAG 
CAGTCCAAGC TACCTGCCAC AGAGAAGCCT GTGCTGCTCT CAAAGGACAT TGAGGCCAAG 
ATGATGGCCC TGGACCGTGA AGTACAGTAT CTGCTTAATA AGGCCAAGTT CGCCAAACCC 
CGGCCCCGGC CCAAGGACAA GAATGGGACC CGGACAGAGC CTCCCGTCAA TGCCAGTGAC 
CAGGGGGAGA AGGTCATCCC TCCAACAGGC CAGACTGAAG ATGCAAAGCC CATTTTGGAA 
CCCGAGAAAG AGTCTGAATC TGAAGTAGCA GATGCTGAAC CTCTGGAGTT AGGAGGCCCT 
GGAGTAGAAA CTGAACAGAA GGAGCAACCA ACAGGACAGA AGAAAACCTT GAAGAATGAT 
GAACTATAA