CRIM1 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRIM1
Entrez Gene ID	100070329
Full Name	cysteine rich transmembrane BMP regulator 1
General protein information	Preferred Names cysteine rich transmembrane BMP regulator 1 Names cysteine-rich motor neuron 1 protein
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005600291.2	XP_005600348.2	cysteine-rich motor neuron 1 protein
XM_005600291.3	XP_005600348.3	cysteine-rich motor neuron 1 protein

Pathways from BioSystems
Gene Ontology

The following CRIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb35475	XM_005600291.2 Latest version!	Equus caballus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OHb50029	XM_005600291.3 Latest version!	Equus caballus cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRIM1 ( XM_005600291.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005600348.2 Equus caballus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.

Price & Availability↑

CloneID	OHb35475
Clone ID Related Accession (Same CDS sequence)	XM_005600291.2
Accession Version	XM_005600291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867379.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005600291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCGCCC GCCAGAGGAA CGAGAGCTGC GGCGGCGCCT ACGGGCTCCA CGGAGCCTGC 
GACAGGGGGC TGCGCTGTGT CATCCGCCCC CCGCTCAATG GCGACTCCAT CACCGAGTAC 
GAAGTGGGCG TCTGCGAAGA TGAGAACTGG GATGATGACC AGCTGCTTGG TTTTGAACCA 
TGCAATGAAA ACCTTGTCTC TGGCTGCAAT ATAATCAATG GGAAATGTGA ATGTGACACC 
ATTCGAACTT GCAACAATCC CTTTGAGTTT CCAAGGAAGG ATATGTGCCT TTCAGCTTTG 
AAGAGGATTG AAGAAGAGAA GCCCGATTGC TCCAAGGCCC GCTGTGAAGT CCAGTTCTCT 
CCACGTTGTC CTGAAGATTC TGTTCTGATC GAGGGCTATG CGCCCCCGGG GGAGTGCTGC 
CCCTTACCCA GCCGCTGCGT GTGCGACCCT GCGGGCTGTC TGCGCAAAGT CTGCCAGCCA 
GGCTACCTGA ACATACTGGT GTCCAAAGCC TCAGGGAAGC CGGGAGAGTG CTGTGACCTC 
TATGAGTGCA AGCCAGTCTT CAGCGTGGAC TGCAGCACCG TGGAGTGCCC CTCCGTTCAG 
CAGGCCGTAT GCCCCCTGGA TAGCTACGAA ACTCAAGTCA GACTCACCGC GGACGGTTGC 
TGCACCCTGC CAACAAGATG CGAGTGTCTC TCTGGCTTAT GTGGTTTCCC CGTGTGTGAG 
GTGGGATCCA CTCCCCGCAT AGTCTCTCGT GGAGATGGGA CACCTGGAAA GTGCTGTGAT 
GTCTTTGAAT GTGTTAATGA AACAAAGCCA GCCTGTGTAT TCAACAATGT GGAATATAAT 
GATGGGGATA TGTTTCGAAT GGACAACTGT CGATTTTGTC GATGCCAAGG GGGTGTTTCC 
ATCTGCTTCA CTGCCCAGTG CGGTGAGCTG AACTGCGAGA GGTACTATGT GCCTGAAGGG 
GAGTGCTGCC CAGTGTGCGA AGATCCAGTG TATCCTTTTA ATAATCCTGC GGGCTGCTAT 
GCCAACGGTC AGATCCGCGC CCATGGGGAC CGGTGGCGGG AAGACGACTG CACGTTCTGC 
CAGTGCATCA ACGGAGAGCC CCACTGCGTT GCAACAGCCT GCGGACAGAG CTGCATGAAC 
CCTGTGAAAG TGCCTGGGGA GTGTTGCCCC GTGTGTGAAG AACCCACCTA CATCACAATT 
GATCCACCTG CATGTGGGGA GTTATCCAAC TGCACCCTGA CAGAGAAGGA CTGCATTTAT 
GGTTTCAAAC TTGATCACAA TGGTTGTCGA ACTTGTCAGT GCAAGAACAG GGAGGAGCTG 
TGCACCGGCC TCAAACGAGG CTGCACCCTG GACTGCCCCT TTGGTTTCCT GACCGATGCC 
CACAACTGTG AGATCTGTCA GTGCCGCCCG CGGCCCAAGA AGTGCAGACC CATAATCTGT 
GACAAGTATT GTCCATTTGG ATACCTGAAG AATAAGCACG GCTGTGACAT CTGTCGCTGT 
AAGAAATGCC CAGAGCTCCC ATGCAGTAAA ATCTGCCCCT TGGGTTTCCA GCAGGACAGT 
CATGGCTGTC TTATCTGCAA GTGCAGAGAG GTCCCTGCTT CAGCCGGGCC ACCTGTCCTG 
TCAGGCACAT GTCTGTCCAT GGATGGCCAT CATCATAAAA ATGAGGAAAG CTGGCATGAT 
GGGTGCCGGG AATGCTACTG TCACAATGGA CGGGAAATGT GTGCTCTGAT CACCTGCCCA 
GTGCCTGCCT GTGGCAACCC CACCATTCAT CCAGGACAGT GCTGCCCGTC CTGCTCAGAT 
GACTTTGTGG TGCAGAAGCC AGAGCTCAGC ACCCCCTCCA TTTGCCACGC CCCTGGAGGA 
GAATACTTTG TGGAAGGAGA AACGTGGAAC ATCGACTCCT GTACTCAGTG CACGTGCCAC 
AGTGGGCGGG TACTGTGTGA GACAGAGGTG TGCCCACCAC TGCTCTGCCA GAACCCCTCA 
CGCACGCAGG ACTCCTGCTG CCCGCAGTGT ACAGATGAAC CTCTCCAGCC TTCCTCATCC 
CATAATGAGA GCGTGCCTAG TTACTGCAAA AATGACGAAG GGGATATATT CCTGGCAGCT 
GAGTCCTGGA AGCCTGACGT TTGTACCAGC TGCGTGTGCA TGGATAGCGT AATTAGCTGT 
TACTCTGAGT CTTGCCCTTC AGTATCCTGT GAAAGACCTG TTTTGAGAAA AGGCCAGTGT 
TGTCCCTACT GCATAGAAGA CACGATTGCA AAGAAGGTGG TGTGCCACTT CAGTGGGAAG 
GCCTACGCCG ACGAGGAGCG GTGGGACATT GACAGCTGCA CACACTGCTA CTGCCTGCAG 
GGCCAGACCC TCTGCTCAAC CGTGAGCTGC CCGCCCCTGC CCTGCGTCGA GCCCATCAAC 
GTGGAAGGAA GTTGCTGCCC AATGTGTCCA GAAATGTATG TCCCAGAACC AACCAATATA 
CCCATAGAGA AGACGAATCA TCGTGGAGAG ATTGACCTGG AGGTTCCTGT GTGGCCCACA 
CCCAGTGAAA ATGACATCAT CCATCTCCCC AGAGACATGG GTCACCTCCA GGTAGATTAC 
AGAGATAATA ACAGGCTGCA CCCAGGCGAA GATTCTTCGC TGGACTCCAT TGCCTCGGTT 
GTGGTTCCCA TAATTATAGG CCTGTCTATT ATAATAGCAT TCCTGTTCAT CAATCAGAAG 
AAACAGTGGA TCCCACTGCT TTGCTGGTAT CGAACACCAA CTAAGCCTTC TTCCTTAAAT 
AATCAGCTGG TATCTGTGGA CTGCAAGAAA GGAACCCGAG TCCAGGTGGA CGGTTCCCAG 
AGAATGCTAA GAATTGCTGA ACCAGACGCA AGATTCAGTG GCTTCTACAG CATGCAAAAA 
CAGAACCATC TACAGGCAGA CAACTTCTAC CAAACAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005600348.2
CDS	36..2957
Translation	MCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPIICDKYCPFGYLKNKHGCDICRCKKCPELPCSKICPLGFQQDSHGCLICKCREVPASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCQNPSRTQDSCCPQCTDEPLQPSSSHNESVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIAKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGEIDLEVPVWPTPSENDIIHLPRDMGHLQVDYRDNNRLHPGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDGSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_005600291.2
Organism	Equus caballus(horse)
Definition	Equus caballus cysteine rich transmembrane BMP regulator 1 (chordin-like) (CRIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005600291.2

ORF Insert Sequence:

ATGTGCGCCC GCCAGAGGAA CGAGAGCTGC GGCGGCGCCT ACGGGCTCCA CGGAGCCTGC 
GACAGGGGGC TGCGCTGTGT CATCCGCCCC CCGCTCAATG GCGACTCCAT CACCGAGTAC 
GAAGTGGGCG TCTGCGAAGA TGAGAACTGG GATGATGACC AGCTGCTTGG TTTTGAACCA 
TGCAATGAAA ACCTTGTCTC TGGCTGCAAT ATAATCAATG GGAAATGTGA ATGTGACACC 
ATTCGAACTT GCAACAATCC CTTTGAGTTT CCAAGGAAGG ATATGTGCCT TTCAGCTTTG 
AAGAGGATTG AAGAAGAGAA GCCCGATTGC TCCAAGGCCC GCTGTGAAGT CCAGTTCTCT 
CCACGTTGTC CTGAAGATTC TGTTCTGATC GAGGGCTATG CGCCCCCGGG GGAGTGCTGC 
CCCTTACCCA GCCGCTGCGT GTGCGACCCT GCGGGCTGTC TGCGCAAAGT CTGCCAGCCA 
GGCTACCTGA ACATACTGGT GTCCAAAGCC TCAGGGAAGC CGGGAGAGTG CTGTGACCTC 
TATGAGTGCA AGCCAGTCTT CAGCGTGGAC TGCAGCACCG TGGAGTGCCC CTCCGTTCAG 
CAGGCCGTAT GCCCCCTGGA TAGCTACGAA ACTCAAGTCA GACTCACCGC GGACGGTTGC 
TGCACCCTGC CAACAAGATG CGAGTGTCTC TCTGGCTTAT GTGGTTTCCC CGTGTGTGAG 
GTGGGATCCA CTCCCCGCAT AGTCTCTCGT GGAGATGGGA CACCTGGAAA GTGCTGTGAT 
GTCTTTGAAT GTGTTAATGA AACAAAGCCA GCCTGTGTAT TCAACAATGT GGAATATAAT 
GATGGGGATA TGTTTCGAAT GGACAACTGT CGATTTTGTC GATGCCAAGG GGGTGTTTCC 
ATCTGCTTCA CTGCCCAGTG CGGTGAGCTG AACTGCGAGA GGTACTATGT GCCTGAAGGG 
GAGTGCTGCC CAGTGTGCGA AGATCCAGTG TATCCTTTTA ATAATCCTGC GGGCTGCTAT 
GCCAACGGTC AGATCCGCGC CCATGGGGAC CGGTGGCGGG AAGACGACTG CACGTTCTGC 
CAGTGCATCA ACGGAGAGCC CCACTGCGTT GCAACAGCCT GCGGACAGAG CTGCATGAAC 
CCTGTGAAAG TGCCTGGGGA GTGTTGCCCC GTGTGTGAAG AACCCACCTA CATCACAATT 
GATCCACCTG CATGTGGGGA GTTATCCAAC TGCACCCTGA CAGAGAAGGA CTGCATTTAT 
GGTTTCAAAC TTGATCACAA TGGTTGTCGA ACTTGTCAGT GCAAGAACAG GGAGGAGCTG 
TGCACCGGCC TCAAACGAGG CTGCACCCTG GACTGCCCCT TTGGTTTCCT GACCGATGCC 
CACAACTGTG AGATCTGTCA GTGCCGCCCG CGGCCCAAGA AGTGCAGACC CATAATCTGT 
GACAAGTATT GTCCATTTGG ATACCTGAAG AATAAGCACG GCTGTGACAT CTGTCGCTGT 
AAGAAATGCC CAGAGCTCCC ATGCAGTAAA ATCTGCCCCT TGGGTTTCCA GCAGGACAGT 
CATGGCTGTC TTATCTGCAA GTGCAGAGAG GTCCCTGCTT CAGCCGGGCC ACCTGTCCTG 
TCAGGCACAT GTCTGTCCAT GGATGGCCAT CATCATAAAA ATGAGGAAAG CTGGCATGAT 
GGGTGCCGGG AATGCTACTG TCACAATGGA CGGGAAATGT GTGCTCTGAT CACCTGCCCA 
GTGCCTGCCT GTGGCAACCC CACCATTCAT CCAGGACAGT GCTGCCCGTC CTGCTCAGAT 
GACTTTGTGG TGCAGAAGCC AGAGCTCAGC ACCCCCTCCA TTTGCCACGC CCCTGGAGGA 
GAATACTTTG TGGAAGGAGA AACGTGGAAC ATCGACTCCT GTACTCAGTG CACGTGCCAC 
AGTGGGCGGG TACTGTGTGA GACAGAGGTG TGCCCACCAC TGCTCTGCCA GAACCCCTCA 
CGCACGCAGG ACTCCTGCTG CCCGCAGTGT ACAGATGAAC CTCTCCAGCC TTCCTCATCC 
CATAATGAGA GCGTGCCTAG TTACTGCAAA AATGACGAAG GGGATATATT CCTGGCAGCT 
GAGTCCTGGA AGCCTGACGT TTGTACCAGC TGCGTGTGCA TGGATAGCGT AATTAGCTGT 
TACTCTGAGT CTTGCCCTTC AGTATCCTGT GAAAGACCTG TTTTGAGAAA AGGCCAGTGT 
TGTCCCTACT GCATAGAAGA CACGATTGCA AAGAAGGTGG TGTGCCACTT CAGTGGGAAG 
GCCTACGCCG ACGAGGAGCG GTGGGACATT GACAGCTGCA CACACTGCTA CTGCCTGCAG 
GGCCAGACCC TCTGCTCAAC CGTGAGCTGC CCGCCCCTGC CCTGCGTCGA GCCCATCAAC 
GTGGAAGGAA GTTGCTGCCC AATGTGTCCA GAAATGTATG TCCCAGAACC AACCAATATA 
CCCATAGAGA AGACGAATCA TCGTGGAGAG ATTGACCTGG AGGTTCCTGT GTGGCCCACA 
CCCAGTGAAA ATGACATCAT CCATCTCCCC AGAGACATGG GTCACCTCCA GGTAGATTAC 
AGAGATAATA ACAGGCTGCA CCCAGGCGAA GATTCTTCGC TGGACTCCAT TGCCTCGGTT 
GTGGTTCCCA TAATTATAGG CCTGTCTATT ATAATAGCAT TCCTGTTCAT CAATCAGAAG 
AAACAGTGGA TCCCACTGCT TTGCTGGTAT CGAACACCAA CTAAGCCTTC TTCCTTAAAT 
AATCAGCTGG TATCTGTGGA CTGCAAGAAA GGAACCCGAG TCCAGGTGGA CGGTTCCCAG 
AGAATGCTAA GAATTGCTGA ACCAGACGCA AGATTCAGTG GCTTCTACAG CATGCAAAAA 
CAGAACCATC TACAGGCAGA CAACTTCTAC CAAACAGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRIM1 ( XM_005600291.3 ) cDNA ORF clone, Equus caballus(horse) -> XP_005600348.3 Equus caballus cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.

Price & Availability↑

CloneID	OHb50029
Clone ID Related Accession (Same CDS sequence)	XM_005600291.3
Accession Version	XM_005600291.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	cysteine-rich motor neuron 1 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009158.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005600291.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGC TGCTGTTGCT GGCGCGCTCC GGCACCCAGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACAGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CATGCAATGA AAACCTTGTC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAATGTGACA CCATTCGAAC TTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCCGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCTGTTCTGA TCGAGGGCTA TGCGCCCCCG 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CAGGCTACCT GAACATACTG GTGTCCAAAG CCTCAGGGAA GCCGGGAGAG 
TGCTGTGACC TCTATGAGTG CAAGCCAGTC TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT ATGCCCCCTG GATAGCTACG AAACTCAAGT CAGACTCACC 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAT 
GTGGAATATA ATGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTTTG TCGATGCCAA 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGCGGTGAGC TGAACTGCGA GAGGTACTAT 
GTGCCTGAAG GGGAGTGCTG CCCAGTGTGC GAAGATCCAG TGTATCCTTT TAATAATCCT 
GCGGGCTGCT ATGCCAACGG TCAGATCCGC GCCCATGGGG ACCGGTGGCG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAACGGAGAG CCCCACTGCG TTGCAACAGC CTGCGGACAG 
AGCTGCATGA ACCCTGTGAA AGTGCCTGGG GAGTGTTGCC CCGTGTGTGA AGAACCCACC 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCCA ACTGCACCCT GACAGAGAAG 
GACTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAGAAC 
AGGGAGGAGC TGTGCACCGG CCTCAAACGA GGCTGCACCC TGGACTGCCC CTTTGGTTTC 
CTGACCGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CGCGGCCCAA GAAGTGCAGA 
CCCATAATCT GTGACAAGTA TTGTCCATTT GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG CCCAGAGCTC CCATGCAGTA AAATCTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CAGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCAGGACA GTGCTGCCCG 
TCCTGCTCAG ATGACTTTGT GGTGCAGAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAC 
GCCCCTGGAG GAGAATACTT TGTGGAAGGA GAAACGTGGA ACATCGACTC CTGTACTCAG 
TGCACGTGCC ACAGTGGGCG GGTACTGTGT GAGACAGAGG TGTGCCCACC ACTGCTCTGC 
CAGAACCCCT CACGCACGCA GGACTCCTGC TGCCCGCAGT GTACAGATGA ACCTCTCCAG 
CCTTCCTCAT CCCATAATGA GAGCGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCTGAC GTTTGTACCA GCTGCGTGTG CATGGATAGC 
GTAATTAGCT GTTACTCTGA GTCTTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATAGAA GACACGATTG CAAAGAAGGT GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAG CGGTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCA ACCGTGAGCT GCCCGCCCCT GCCCTGCGTC 
GAGCCCATCA ACGTGGAAGG AAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATAGA GAAGACGAAT CATCGTGGAG AGATTGACCT GGAGGTTCCT 
GTGTGGCCCA CACCCAGTGA AAATGACATC ATCCATCTCC CCAGAGACAT GGGTCACCTC 
CAGGTAGATT ACAGAGATAA TAACAGGCTG CACCCAGGCG AAGATTCTTC GCTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATAATTATA GGCCTGTCTA TTATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATCCCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACCCG AGTCCAGGTG 
GACGGTTCCC AGAGAATGCT AAGAATTGCT GAACCAGACG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAGAACCA TCTACAGGCA GACAACTTCT ACCAAACAGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005600348.3
CDS	1..3114
Translation	MYLVAGGRGLAGCGHLLVSLLGLLLLLARSGTQALVCLPCDESKCEEPRSCPGSIVQGVCGCCYMCARQRNESCGGAYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLLGFEPCNENLVSGCNIINGKCECDTIRTCNNPFEFPRKDMCLSALKRIEEEKPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCDPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPSVQQAVCPLDSYETQVRLTADGCCTLPTRCECLSGLCGFPVCEVGSTPRIVSRGDGTPGKCCDVFECVNETKPACVFNNVEYNDGDMFRMDNCRFCRCQGGVSICFTAQCGELNCERYYVPEGECCPVCEDPVYPFNNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCMNPVKVPGECCPVCEEPTYITIDPPACGELSNCTLTEKDCIYGFKLDHNGCRTCQCKNREELCTGLKRGCTLDCPFGFLTDAHNCEICQCRPRPKKCRPIICDKYCPFGYLKNKHGCDICRCKKCPELPCSKICPLGFQQDSHGCLICKCREVPASAGPPVLSGTCLSMDGHHHKNEESWHDGCRECYCHNGREMCALITCPVPACGNPTIHPGQCCPSCSDDFVVQKPELSTPSICHAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCQNPSRTQDSCCPQCTDEPLQPSSSHNESVPSYCKNDEGDIFLAAESWKPDVCTSCVCMDSVISCYSESCPSVSCERPVLRKGQCCPYCIEDTIAKKVVCHFSGKAYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPINVEGSCCPMCPEMYVPEPTNIPIEKTNHRGEIDLEVPVWPTPSENDIIHLPRDMGHLQVDYRDNNRLHPGEDSSLDSIASVVVPIIIGLSIIIAFLFINQKKQWIPLLCWYRTPTKPSSLNNQLVSVDCKKGTRVQVDGSQRMLRIAEPDARFSGFYSMQKQNHLQADNFYQTV

Target ORF information:

RefSeq Version	XM_005600291.3
Organism	Equus caballus(horse)
Definition	Equus caballus cysteine rich transmembrane BMP regulator 1 (CRIM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005600291.3

ORF Insert Sequence:

ATGTACTTGG TGGCGGGGGG CAGGGGGCTG GCCGGCTGCG GGCACCTCCT GGTCTCGCTG 
CTGGGGCTGC TGCTGTTGCT GGCGCGCTCC GGCACCCAGG CGCTGGTCTG CCTGCCCTGC 
GACGAGTCCA AGTGCGAAGA GCCCAGGAGC TGCCCCGGGA GCATCGTGCA GGGCGTCTGC 
GGCTGCTGCT ACATGTGCGC CCGCCAGAGG AACGAGAGCT GCGGCGGCGC CTACGGGCTC 
CACGGAGCCT GCGACAGGGG GCTGCGCTGT GTCATCCGCC CCCCGCTCAA TGGCGACTCC 
ATCACCGAGT ACGAAGTGGG CGTCTGCGAA GATGAGAACT GGGATGATGA CCAGCTGCTT 
GGTTTTGAAC CATGCAATGA AAACCTTGTC TCTGGCTGCA ATATAATCAA TGGGAAATGT 
GAATGTGACA CCATTCGAAC TTGCAACAAT CCCTTTGAGT TTCCAAGGAA GGATATGTGC 
CTTTCAGCTT TGAAGAGGAT TGAAGAAGAG AAGCCCGATT GCTCCAAGGC CCGCTGTGAA 
GTCCAGTTCT CTCCACGTTG TCCTGAAGAT TCTGTTCTGA TCGAGGGCTA TGCGCCCCCG 
GGGGAGTGCT GCCCCTTACC CAGCCGCTGC GTGTGCGACC CTGCGGGCTG TCTGCGCAAA 
GTCTGCCAGC CAGGCTACCT GAACATACTG GTGTCCAAAG CCTCAGGGAA GCCGGGAGAG 
TGCTGTGACC TCTATGAGTG CAAGCCAGTC TTCAGCGTGG ACTGCAGCAC CGTGGAGTGC 
CCCTCCGTTC AGCAGGCCGT ATGCCCCCTG GATAGCTACG AAACTCAAGT CAGACTCACC 
GCGGACGGTT GCTGCACCCT GCCAACAAGA TGCGAGTGTC TCTCTGGCTT ATGTGGTTTC 
CCCGTGTGTG AGGTGGGATC CACTCCCCGC ATAGTCTCTC GTGGAGATGG GACACCTGGA 
AAGTGCTGTG ATGTCTTTGA ATGTGTTAAT GAAACAAAGC CAGCCTGTGT ATTCAACAAT 
GTGGAATATA ATGATGGGGA TATGTTTCGA ATGGACAACT GTCGATTTTG TCGATGCCAA 
GGGGGTGTTT CCATCTGCTT CACTGCCCAG TGCGGTGAGC TGAACTGCGA GAGGTACTAT 
GTGCCTGAAG GGGAGTGCTG CCCAGTGTGC GAAGATCCAG TGTATCCTTT TAATAATCCT 
GCGGGCTGCT ATGCCAACGG TCAGATCCGC GCCCATGGGG ACCGGTGGCG GGAAGACGAC 
TGCACGTTCT GCCAGTGCAT CAACGGAGAG CCCCACTGCG TTGCAACAGC CTGCGGACAG 
AGCTGCATGA ACCCTGTGAA AGTGCCTGGG GAGTGTTGCC CCGTGTGTGA AGAACCCACC 
TACATCACAA TTGATCCACC TGCATGTGGG GAGTTATCCA ACTGCACCCT GACAGAGAAG 
GACTGCATTT ATGGTTTCAA ACTTGATCAC AATGGTTGTC GAACTTGTCA GTGCAAGAAC 
AGGGAGGAGC TGTGCACCGG CCTCAAACGA GGCTGCACCC TGGACTGCCC CTTTGGTTTC 
CTGACCGATG CCCACAACTG TGAGATCTGT CAGTGCCGCC CGCGGCCCAA GAAGTGCAGA 
CCCATAATCT GTGACAAGTA TTGTCCATTT GGATACCTGA AGAATAAGCA CGGCTGTGAC 
ATCTGTCGCT GTAAGAAATG CCCAGAGCTC CCATGCAGTA AAATCTGCCC CTTGGGTTTC 
CAGCAGGACA GTCATGGCTG TCTTATCTGC AAGTGCAGAG AGGTCCCTGC TTCAGCCGGG 
CCACCTGTCC TGTCAGGCAC ATGTCTGTCC ATGGATGGCC ATCATCATAA AAATGAGGAA 
AGCTGGCATG ATGGGTGCCG GGAATGCTAC TGTCACAATG GACGGGAAAT GTGTGCTCTG 
ATCACCTGCC CAGTGCCTGC CTGTGGCAAC CCCACCATTC ATCCAGGACA GTGCTGCCCG 
TCCTGCTCAG ATGACTTTGT GGTGCAGAAG CCAGAGCTCA GCACCCCCTC CATTTGCCAC 
GCCCCTGGAG GAGAATACTT TGTGGAAGGA GAAACGTGGA ACATCGACTC CTGTACTCAG 
TGCACGTGCC ACAGTGGGCG GGTACTGTGT GAGACAGAGG TGTGCCCACC ACTGCTCTGC 
CAGAACCCCT CACGCACGCA GGACTCCTGC TGCCCGCAGT GTACAGATGA ACCTCTCCAG 
CCTTCCTCAT CCCATAATGA GAGCGTGCCT AGTTACTGCA AAAATGACGA AGGGGATATA 
TTCCTGGCAG CTGAGTCCTG GAAGCCTGAC GTTTGTACCA GCTGCGTGTG CATGGATAGC 
GTAATTAGCT GTTACTCTGA GTCTTGCCCT TCAGTATCCT GTGAAAGACC TGTTTTGAGA 
AAAGGCCAGT GTTGTCCCTA CTGCATAGAA GACACGATTG CAAAGAAGGT GGTGTGCCAC 
TTCAGTGGGA AGGCCTACGC CGACGAGGAG CGGTGGGACA TTGACAGCTG CACACACTGC 
TACTGCCTGC AGGGCCAGAC CCTCTGCTCA ACCGTGAGCT GCCCGCCCCT GCCCTGCGTC 
GAGCCCATCA ACGTGGAAGG AAGTTGCTGC CCAATGTGTC CAGAAATGTA TGTCCCAGAA 
CCAACCAATA TACCCATAGA GAAGACGAAT CATCGTGGAG AGATTGACCT GGAGGTTCCT 
GTGTGGCCCA CACCCAGTGA AAATGACATC ATCCATCTCC CCAGAGACAT GGGTCACCTC 
CAGGTAGATT ACAGAGATAA TAACAGGCTG CACCCAGGCG AAGATTCTTC GCTGGACTCC 
ATTGCCTCGG TTGTGGTTCC CATAATTATA GGCCTGTCTA TTATAATAGC ATTCCTGTTC 
ATCAATCAGA AGAAACAGTG GATCCCACTG CTTTGCTGGT ATCGAACACC AACTAAGCCT 
TCTTCCTTAA ATAATCAGCT GGTATCTGTG GACTGCAAGA AAGGAACCCG AGTCCAGGTG 
GACGGTTCCC AGAGAATGCT AAGAATTGCT GAACCAGACG CAAGATTCAG TGGCTTCTAC 
AGCATGCAAA AACAGAACCA TCTACAGGCA GACAACTTCT ACCAAACAGT GTGA