NUB1 cDNA ORF clone, Equus caballus(horse)

The following NUB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb20133 XM_005609543.2
Latest version!
Equus caballus negative regulator of ubiquitin-like proteins 1 (NUB1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHb64546 XM_005609543.3
Latest version!
Equus caballus negative regulator of ubiquitin like proteins 1 (NUB1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb20133
    Clone ID Related Accession (Same CDS sequence) XM_005609543.2
    Accession Version XM_005609543.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: NEDD8 ultimate buster 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867415.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005609543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAACAA ACGGTGGCCC CCGCCGCATC CGGGCACTCG GTGAGTCGCG ACGGGAGTGG 
    AGGGGAGCTC TAATGGCGCA AAAGGAATAT CTCCAAGCAA AATTGACCCA GTTTTTAAGG
    GAAGACAGAA TTCAACTTTG GAAACCTCCA TATACAGATG AAAATAAAGA AGTTGGTTTG
    GCGTTGAAGG ATCTTGCTAA GAAGTACTCT GACAAGCTAG AATGTTGTGA AAATGAAGTA
    GAAAAGATAA TGGAAGAAAT ACGTTGCAAA GCAATCGAGC GTGGCACAGG AAATGAACAT
    TATAAAACGA CAGGAATTGC TACAATTGAG GTATTTTTAC CTCCACGAGT AAGAAAAGAT
    AGAAAAAACC GGTTGGAGAC CCGATTGCAC ATCACTGGCA GGGAACTGAG GTCTAAAATA
    GCTGAAACCT TTGGATTTCA AGAAAGTTAT ATCAAAATTG TCATAAATAA GAAACAACTT
    CAACTAGGGA GAACACTTGA GGAACAAGGA GTGACACACA ATGTCAAAGC TATGGTGCTC
    GAACTAAAAC AGTCTGAAGA GGATACAAGA AAAAATGTCC AGGTTGAAGA AGACGAGCAA
    AATGAAGCCA AACTAAAAGA AAAAAGAATT CAGAGGACTA AGAGAGGACT GGAGATCCTG
    GCAGAGAGAG CTGAGATGGT GGTGGATCCA GAAACTACCC CGTACTTAGA CATAGCCAAC
    CAGACGGGCA GGTCCATCAG AATGCCTCCA TCTGAAAGAA AAGCCCTTGT GTTAGCTATG
    GGATATCATG AGAAGGGCAG AGCTTTCCTG AAAAGAAAAG AATATGGAAT AGCCTTGCCA
    TGTTTGTTGG ACGCTGACAG ATATTTCTGT GAGTGTTGCC CAGAACTACT GGATACGGTA
    GATAACTATG CAGTGCTCCA GCTGGATATA GTGTGGTGTT ACTTCCGCTT GGGACAACTG
    GAATGCCTTG ATGATGCAGA AAAAAAATTA AACTTGGCCC AGAAATGCTT TAAAAATTGT
    TATGGAGAAA ATCATCAGAG ACTGGTCCAC ATAAAAGGAA ATTGTGGAAA AGAGAAGATA
    CTGTTTTTAA GACTTTACTT GCTTCAGGGT ATCCGAAATT ATCTCAGTGG GAATGGTGAA
    GTGGCTTGTG ACTATCTCAG TAAGGCACAT CAACTCTTTA AAGAGCTATA TATCGATCCA
    ACAAAAGTTG ACAATTTGTT GCAGTTGGGA TTTACTGCCC AGGAAGCCCG GCTTGGCCTG
    AGGGCATGTG ACGGGAATGT GGATCACGCA GCCGCCCACA TTACCAACCG CAGAGAGGAA
    TTGGCCCAAA TAAGGAAAGA GGAAAAAGAG AAGAAAAGAC GCCGCCTAGA AAACATAAAC
    TCTTTGAAAG GAATGGGCTA CTCCACCCAT GCAGCCAGGC AGGCTCTCCA TCAGGCCAGC
    GGGAACCTGG ACGAAGCGCT GAAGATTCTT GTCAGTAATC CTCAGATGTG GTGGTTAAAT
    GATTCGGATC CTCAAACCAA CAACTGTCAA GAAAGTCCTT CCCAGGAAAG TATCAACCAA
    CTGGTGTACA TGGGCTTTGA AGCGGTAGCG GCCGAAGCTG CCCTGAGGGT GTTCAGGGGA
    AACGTCCAGC TGGCAGCTCA GACCCTTGCC CACAATGGAG GAAGTCTTCC TCCCGACCTC
    CAGTTTTCGG CGGAAGATTC TTCATCAACG CCGTCCACGT CCCCTTCCGA TTCTGCAGCT
    GCCTCTAGTG CCTCAACAGA TGAAGATATG GAGACAGAGG CCGTCAATGA AATATTGGAA
    GATATTCCAG AACATGAAGA AGATTATCTT GACTCAACTC TGGAAGACGA AGAAATTCTT
    ATTGCAGAGT ACTTATGCTA TGTAGAAAAT ATAAAGTCAG CAACCAAGAA AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005609600.1
    CDS16..1932
    Translation

    Target ORF information:

    RefSeq Version XM_005609543.2
    Organism Equus caballus(horse)
    Definition Equus caballus negative regulator of ubiquitin-like proteins 1 (NUB1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005609543.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAACAA ACGGTGGCCC CCGCCGCATC CGGGCACTCG GTGAGTCGCG ACGGGAGTGG 
    AGGGGAGCTC TAATGGCGCA AAAGGAATAT CTCCAAGCAA AATTGACCCA GTTTTTAAGG
    GAAGACAGAA TTCAACTTTG GAAACCTCCA TATACAGATG AAAATAAAGA AGTTGGTTTG
    GCGTTGAAGG ATCTTGCTAA GAAGTACTCT GACAAGCTAG AATGTTGTGA AAATGAAGTA
    GAAAAGATAA TGGAAGAAAT ACGTTGCAAA GCAATCGAGC GTGGCACAGG AAATGAACAT
    TATAAAACGA CAGGAATTGC TACAATTGAG GTATTTTTAC CTCCACGAGT AAGAAAAGAT
    AGAAAAAACC GGTTGGAGAC CCGATTGCAC ATCACTGGCA GGGAACTGAG GTCTAAAATA
    GCTGAAACCT TTGGATTTCA AGAAAGTTAT ATCAAAATTG TCATAAATAA GAAACAACTT
    CAACTAGGGA GAACACTTGA GGAACAAGGA GTGACACACA ATGTCAAAGC TATGGTGCTC
    GAACTAAAAC AGTCTGAAGA GGATACAAGA AAAAATGTCC AGGTTGAAGA AGACGAGCAA
    AATGAAGCCA AACTAAAAGA AAAAAGAATT CAGAGGACTA AGAGAGGACT GGAGATCCTG
    GCAGAGAGAG CTGAGATGGT GGTGGATCCA GAAACTACCC CGTACTTAGA CATAGCCAAC
    CAGACGGGCA GGTCCATCAG AATGCCTCCA TCTGAAAGAA AAGCCCTTGT GTTAGCTATG
    GGATATCATG AGAAGGGCAG AGCTTTCCTG AAAAGAAAAG AATATGGAAT AGCCTTGCCA
    TGTTTGTTGG ACGCTGACAG ATATTTCTGT GAGTGTTGCC CAGAACTACT GGATACGGTA
    GATAACTATG CAGTGCTCCA GCTGGATATA GTGTGGTGTT ACTTCCGCTT GGGACAACTG
    GAATGCCTTG ATGATGCAGA AAAAAAATTA AACTTGGCCC AGAAATGCTT TAAAAATTGT
    TATGGAGAAA ATCATCAGAG ACTGGTCCAC ATAAAAGGAA ATTGTGGAAA AGAGAAGATA
    CTGTTTTTAA GACTTTACTT GCTTCAGGGT ATCCGAAATT ATCTCAGTGG GAATGGTGAA
    GTGGCTTGTG ACTATCTCAG TAAGGCACAT CAACTCTTTA AAGAGCTATA TATCGATCCA
    ACAAAAGTTG ACAATTTGTT GCAGTTGGGA TTTACTGCCC AGGAAGCCCG GCTTGGCCTG
    AGGGCATGTG ACGGGAATGT GGATCACGCA GCCGCCCACA TTACCAACCG CAGAGAGGAA
    TTGGCCCAAA TAAGGAAAGA GGAAAAAGAG AAGAAAAGAC GCCGCCTAGA AAACATAAAC
    TCTTTGAAAG GAATGGGCTA CTCCACCCAT GCAGCCAGGC AGGCTCTCCA TCAGGCCAGC
    GGGAACCTGG ACGAAGCGCT GAAGATTCTT GTCAGTAATC CTCAGATGTG GTGGTTAAAT
    GATTCGGATC CTCAAACCAA CAACTGTCAA GAAAGTCCTT CCCAGGAAAG TATCAACCAA
    CTGGTGTACA TGGGCTTTGA AGCGGTAGCG GCCGAAGCTG CCCTGAGGGT GTTCAGGGGA
    AACGTCCAGC TGGCAGCTCA GACCCTTGCC CACAATGGAG GAAGTCTTCC TCCCGACCTC
    CAGTTTTCGG CGGAAGATTC TTCATCAACG CCGTCCACGT CCCCTTCCGA TTCTGCAGCT
    GCCTCTAGTG CCTCAACAGA TGAAGATATG GAGACAGAGG CCGTCAATGA AATATTGGAA
    GATATTCCAG AACATGAAGA AGATTATCTT GACTCAACTC TGGAAGACGA AGAAATTCTT
    ATTGCAGAGT ACTTATGCTA TGTAGAAAAT ATAAAGTCAG CAACCAAGAA AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64546
    Clone ID Related Accession (Same CDS sequence) XM_005609543.3
    Accession Version XM_005609543.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: NEDD8 ultimate buster 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009147.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005609543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAACAA ACGGTGGCCC CCGCCGCATC CGGGCACTCG GTGAGTCGCG ACGGGAGTGG 
    AGGGGAGCTC TAATGGCGCA AAAGGAATAT CTCCAAGCAA AATTGACCCA GTTTTTAAGG
    GAAGACAGAA TTCAACTTTG GAAACCTCCA TATACAGATG AAAATAAAGA AGTTGGTTTG
    GCGTTGAAGG ATCTTGCTAA GAAGTACTCT GACAAGCTAG AATGTTGTGA AAATGAAGTA
    GAAAAGATAA TGGAAGAAAT ACGTTGCAAA GCAATCGAGC GTGGCACAGG AAATGAACAT
    TATAAAACGA CAGGAATTGC TACAATTGAG GTATTTTTAC CTCCACGAGT AAGAAAAGAT
    AGAAAAAACC GGTTGGAGAC CCGATTGCAC ATCACTGGCA GGGAACTGAG GTCTAAAATA
    GCTGAAACCT TTGGATTTCA AGAAAGTTAT ATCAAAATTG TCATAAATAA GAAACAACTT
    CAACTAGGGA GAACACTTGA GGAACAAGGA GTGACACACA ATGTCAAAGC TATGGTGCTC
    GAACTAAAAC AGTCTGAAGA GGATACAAGA AAAAATGTCC AGGTTGAAGA AGACGAGCAA
    AATGAAGCCA AACTAAAAGA AAAAAGAATT CAGAGGACTA AGAGAGGACT GGAGATCCTG
    GCAGAGAGAG CTGAGATGGT GGTGGATCCA GAAACTACCC CATACTTAGA CATAGCTAAC
    CAGACGGGCA GGTCCATCAG AATGCCTCCA TCTGAAAGAA AAGCCCTTGT GTTAGCTATG
    GGATATCATG AGAAGGGCAG AGCTTTCCTG AAAAGAAAAG AATATGGAAT AGCCTTGCCA
    TGTTTGTTGG ACGCTGACAG ATATTTCTGT GAGTGTTGCC CAGAACTACT GGATACGGTA
    GATAACTATG CAGTGCTCCA GCTGGATATA GTGTGGTGTT ACTTCCGCTT GGGACAACTG
    GAATGCCTTG ATGATGCAGA AAAAAAATTA AACTTGGCCC AGAAATGCTT TAAAAATTGT
    TATGGAGAAA ATCATCAGAG ACTGGTCCAC ATAAAAGGAA ATTGTGGAAA AGAGAAGATA
    CTGTTTTTAA GACTTTACTT GCTTCAGGGT ATCCGAAATT ATCTCAGTGG GAATGGTGAA
    GTGGCTTGTG ACTATCTCAG TAAGGCACAT CAACTCTTTA AAGAGCTATA TATCGATCCA
    ACAAAAGTTG ACAATTTGTT GCAGTTGGGA TTTACTGCCC AGGAAGCCCG GCTTGGCCTG
    AGGGCATGTG ACGGGAATGT GGATCACGCA GCCGCCCACA TTACCAACCG CAGAGAGGAA
    TTGGCCCAAA TAAGGAAAGA GGAAAAAGAG AAGAAAAGAC GCCGCCTAGA AAACATAAAC
    TCTTTGAAAG GAATGGGCTA CTCCACCCAT GCAGCCAGGC AGGCTCTCCA TCAGGCCAGC
    GGGAACCTGG ACGAAGCGCT GAAGATTCTT GTCAGTAATC CTCAGATGTG GTGGTTAAAT
    GATTCGGATC CTCAAACCAA CAACTGTCAA GAAAGTCCTT CCCAGGAAAG TATCAACCAA
    CTGGTGTACA TGGGCTTTGA AGCGGTAGCG GCCGAAGCTG CCCTGAGGGT GTTCAGGGGA
    AACGTCCAGC TGGCAGCTCA GACCCTTGCC CACAATGGAG GAAGTCTTCC TCCCGACCTC
    CAGTTTTCGG CGGAAGATTC TTCATCAACG CCGTCCACGT CCCCTTCCGA TTCTGCAGCT
    GCCTCTAGTG CCTCAACAGA TGAAGATATG GAGACAGAGG CCGTCAATGA AATATTGGAA
    GATATTCCAG AACATGAAGA AGATTATCTT GACTCAACTC TGGAAGACGA AGAAATTCTT
    ATTGCAGAGT ACTTATGCTA TGTAGAAAAT ATAAAGTCAG CAACCAAGAA AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005609600.1
    CDS16..1932
    Translation

    Target ORF information:

    RefSeq Version XM_005609543.3
    Organism Equus caballus(horse)
    Definition Equus caballus negative regulator of ubiquitin like proteins 1 (NUB1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005609543.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAAAACAA ACGGTGGCCC CCGCCGCATC CGGGCACTCG GTGAGTCGCG ACGGGAGTGG 
    AGGGGAGCTC TAATGGCGCA AAAGGAATAT CTCCAAGCAA AATTGACCCA GTTTTTAAGG
    GAAGACAGAA TTCAACTTTG GAAACCTCCA TATACAGATG AAAATAAAGA AGTTGGTTTG
    GCGTTGAAGG ATCTTGCTAA GAAGTACTCT GACAAGCTAG AATGTTGTGA AAATGAAGTA
    GAAAAGATAA TGGAAGAAAT ACGTTGCAAA GCAATCGAGC GTGGCACAGG AAATGAACAT
    TATAAAACGA CAGGAATTGC TACAATTGAG GTATTTTTAC CTCCACGAGT AAGAAAAGAT
    AGAAAAAACC GGTTGGAGAC CCGATTGCAC ATCACTGGCA GGGAACTGAG GTCTAAAATA
    GCTGAAACCT TTGGATTTCA AGAAAGTTAT ATCAAAATTG TCATAAATAA GAAACAACTT
    CAACTAGGGA GAACACTTGA GGAACAAGGA GTGACACACA ATGTCAAAGC TATGGTGCTC
    GAACTAAAAC AGTCTGAAGA GGATACAAGA AAAAATGTCC AGGTTGAAGA AGACGAGCAA
    AATGAAGCCA AACTAAAAGA AAAAAGAATT CAGAGGACTA AGAGAGGACT GGAGATCCTG
    GCAGAGAGAG CTGAGATGGT GGTGGATCCA GAAACTACCC CATACTTAGA CATAGCTAAC
    CAGACGGGCA GGTCCATCAG AATGCCTCCA TCTGAAAGAA AAGCCCTTGT GTTAGCTATG
    GGATATCATG AGAAGGGCAG AGCTTTCCTG AAAAGAAAAG AATATGGAAT AGCCTTGCCA
    TGTTTGTTGG ACGCTGACAG ATATTTCTGT GAGTGTTGCC CAGAACTACT GGATACGGTA
    GATAACTATG CAGTGCTCCA GCTGGATATA GTGTGGTGTT ACTTCCGCTT GGGACAACTG
    GAATGCCTTG ATGATGCAGA AAAAAAATTA AACTTGGCCC AGAAATGCTT TAAAAATTGT
    TATGGAGAAA ATCATCAGAG ACTGGTCCAC ATAAAAGGAA ATTGTGGAAA AGAGAAGATA
    CTGTTTTTAA GACTTTACTT GCTTCAGGGT ATCCGAAATT ATCTCAGTGG GAATGGTGAA
    GTGGCTTGTG ACTATCTCAG TAAGGCACAT CAACTCTTTA AAGAGCTATA TATCGATCCA
    ACAAAAGTTG ACAATTTGTT GCAGTTGGGA TTTACTGCCC AGGAAGCCCG GCTTGGCCTG
    AGGGCATGTG ACGGGAATGT GGATCACGCA GCCGCCCACA TTACCAACCG CAGAGAGGAA
    TTGGCCCAAA TAAGGAAAGA GGAAAAAGAG AAGAAAAGAC GCCGCCTAGA AAACATAAAC
    TCTTTGAAAG GAATGGGCTA CTCCACCCAT GCAGCCAGGC AGGCTCTCCA TCAGGCCAGC
    GGGAACCTGG ACGAAGCGCT GAAGATTCTT GTCAGTAATC CTCAGATGTG GTGGTTAAAT
    GATTCGGATC CTCAAACCAA CAACTGTCAA GAAAGTCCTT CCCAGGAAAG TATCAACCAA
    CTGGTGTACA TGGGCTTTGA AGCGGTAGCG GCCGAAGCTG CCCTGAGGGT GTTCAGGGGA
    AACGTCCAGC TGGCAGCTCA GACCCTTGCC CACAATGGAG GAAGTCTTCC TCCCGACCTC
    CAGTTTTCGG CGGAAGATTC TTCATCAACG CCGTCCACGT CCCCTTCCGA TTCTGCAGCT
    GCCTCTAGTG CCTCAACAGA TGAAGATATG GAGACAGAGG CCGTCAATGA AATATTGGAA
    GATATTCCAG AACATGAAGA AGATTATCTT GACTCAACTC TGGAAGACGA AGAAATTCTT
    ATTGCAGAGT ACTTATGCTA TGTAGAAAAT ATAAAGTCAG CAACCAAGAA AAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.