WRN cDNA ORF clone, Equus caballus(horse)

The following WRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb38322 XM_014736585.1
Latest version!
Equus caballus Werner syndrome, RecQ helicase-like (WRN), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OHb60382 XM_023630595.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60382 XM_023630594.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60382 XM_023630593.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60383 XM_014736585.2
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60384 XM_023630596.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60385 XM_023630597.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60386 XM_023630598.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60386 XM_023630599.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb60387 XM_023630600.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb60388 XM_023630601.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb60389 XM_023630602.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb60390 XM_023630603.1
Latest version!
Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb38322
    Clone ID Related Accession (Same CDS sequence) XM_014736585.1
    Accession Version XM_014736585.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: Werner syndrome ATP-dependent helicase
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867398.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTNGAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGNAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGGCTA ACTTGGAGAA GATGTATTCT
    TATAAAGCAT TTGATAAAAT TTCTTCTGGG AGTAACATTC CTAAAAAAAG CGTCATGTTG
    GAATCACCAA GAAAATCTTA CAAGTCCTCA GAACCTGTTA TTTCAGCTCA AGAGCAAGAG
    TCTCAGACTC TGTTATACGG TGAATTGGTA GAAGCTAGGC AGAAACGTGC CCATAACATG
    GACGTTCCTC CAGCTATCCT GGCAACAAAT AAGATACTGT TGGATATGGC CAAAATGAGA
    CCTACTACGG TTGAAAATGT GAAAAGGATC GACGGTGTTT CTGAAGGCAA AGCTACTATG
    TTGGCCCCTC TGTTGGAAGT CATCAAACAT TTCAGTCAAA CAAATAGTAT TCAGACAGAC
    CTCTTTCCAA GTACAAAGCC TCAAGAACAG CAGAAAAGTC CGGTGGTGAA AGATGAAGCA
    CGCTCACTTT CACACTCTGT GGCCGTCACA TATTCTTTAT TCCAAGAAAA GAACATGTCT
    TTGAAAAGCA TATCTGAGAA CAGGACTCTG CCTCTCACAG CAGTTGGCGC ACACTTACTC
    CAAGCGCTGA AAGCCGGCTA CCCACTCGAC ATGGAGCGAG CAGGCCTGAC TCCAGAGACT
    CACAAGATTA TTGCTGATGT CATCCGAAAC CCTCCCGTCA ACTCAGATAT CAATAAAACT
    AAACTAATTA GAATGTTTGT TCCTGAAAAC ATTGACACAT ACCTTATCTA CATGGCAATT
    GAGATCCTGA GAAAAGATTG TGACAACAGA CCGTTATGCC AACCTTCATG TGATTCCAAT
    AAAAAGAGAT GTTTCCCCAA CTCTGAGGAC AGCTGTTCAC GTTCGAAGAG AAGCAAGGAC
    GAAGTAGGCA CTAATACCAA GGTTGATGGA AAACATCCTT GTGATCTTTC ACCAAAAGAT
    AAGAGTCACA GAAATTCACT GAGACTGACA TCTTGGAATC AGCCATCCAG CGATCCAGAA
    ATAGAAGATT TATTTACAGA CTCTCATCCA CAGACTTCAT CTGGAGCCTT AAAGAGAATG
    TATGCGGGGT TTGGCAAGAG AGATAAGATC TTAGTTGATA CCAGCAAAAA ATCAATGGCC
    AGAACGAAAA AGAAGGGTCT TTTTAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014592071.1
    CDS204..4553
    Translation

    Target ORF information:

    RefSeq Version XM_014736585.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome, RecQ helicase-like (WRN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014736585.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTNGAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGNAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGGCTA ACTTGGAGAA GATGTATTCT
    TATAAAGCAT TTGATAAAAT TTCTTCTGGG AGTAACATTC CTAAAAAAAG CGTCATGTTG
    GAATCACCAA GAAAATCTTA CAAGTCCTCA GAACCTGTTA TTTCAGCTCA AGAGCAAGAG
    TCTCAGACTC TGTTATACGG TGAATTGGTA GAAGCTAGGC AGAAACGTGC CCATAACATG
    GACGTTCCTC CAGCTATCCT GGCAACAAAT AAGATACTGT TGGATATGGC CAAAATGAGA
    CCTACTACGG TTGAAAATGT GAAAAGGATC GACGGTGTTT CTGAAGGCAA AGCTACTATG
    TTGGCCCCTC TGTTGGAAGT CATCAAACAT TTCAGTCAAA CAAATAGTAT TCAGACAGAC
    CTCTTTCCAA GTACAAAGCC TCAAGAACAG CAGAAAAGTC CGGTGGTGAA AGATGAAGCA
    CGCTCACTTT CACACTCTGT GGCCGTCACA TATTCTTTAT TCCAAGAAAA GAACATGTCT
    TTGAAAAGCA TATCTGAGAA CAGGACTCTG CCTCTCACAG CAGTTGGCGC ACACTTACTC
    CAAGCGCTGA AAGCCGGCTA CCCACTCGAC ATGGAGCGAG CAGGCCTGAC TCCAGAGACT
    CACAAGATTA TTGCTGATGT CATCCGAAAC CCTCCCGTCA ACTCAGATAT CAATAAAACT
    AAACTAATTA GAATGTTTGT TCCTGAAAAC ATTGACACAT ACCTTATCTA CATGGCAATT
    GAGATCCTGA GAAAAGATTG TGACAACAGA CCGTTATGCC AACCTTCATG TGATTCCAAT
    AAAAAGAGAT GTTTCCCCAA CTCTGAGGAC AGCTGTTCAC GTTCGAAGAG AAGCAAGGAC
    GAAGTAGGCA CTAATACCAA GGTTGATGGA AAACATCCTT GTGATCTTTC ACCAAAAGAT
    AAGAGTCACA GAAATTCACT GAGACTGACA TCTTGGAATC AGCCATCCAG CGATCCAGAA
    ATAGAAGATT TATTTACAGA CTCTCATCCA CAGACTTCAT CTGGAGCCTT AAAGAGAATG
    TATGCGGGGT TTGGCAAGAG AGATAAGATC TTAGTTGATA CCAGCAAAAA ATCAATGGCC
    AGAACGAAAA AGAAGGGTCT TTTTAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60382
    Clone ID Related Accession (Same CDS sequence) XM_023630594.1 , XM_023630593.1 , XM_023630595.1
    Accession Version XM_023630594.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486362.1
    CDS466..4818
    Translation

    Target ORF information:

    RefSeq Version XM_023630594.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630594.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60382
    Clone ID Related Accession (Same CDS sequence) XM_023630594.1 , XM_023630593.1 , XM_023630595.1
    Accession Version XM_023630593.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486361.1
    CDS215..4567
    Translation

    Target ORF information:

    RefSeq Version XM_023630593.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630593.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60382
    Clone ID Related Accession (Same CDS sequence) XM_023630594.1 , XM_023630593.1 , XM_023630595.1
    Accession Version XM_023630595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486363.1
    CDS1435..5787
    Translation

    Target ORF information:

    RefSeq Version XM_023630595.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60383
    Clone ID Related Accession (Same CDS sequence) XM_014736585.2
    Accession Version XM_014736585.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4350bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014736585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGGCTA ACTTGGAGAA GATGTATTCT
    TATAAAGCAT TTGATAAAAT TTCTTCTGGG AGTAACATTC CTAAAAAAAG CGTCATGTTG
    GAATCACCAA GAAAATCTTA CAAGTCCTCA GAACCTGTTA TTTCAGCTCA AGAGCAAGAG
    TCTCAGACTC TGTTATACGG TGAATTGGTA GAAGCTAGGC AGAAACGTGC CCATAACATG
    GACGTTCCTC CAGCTATCCT GGCAACAAAT AAGATACTGT TGGATATGGC CAAAATGAGA
    CCTACTACGG TTGAAAATGT GAAAAGGATC GACGGTGTTT CTGAAGGCAA AGCTACTATG
    TTGGCCCCTC TGTTGGAAGT CATCAAACAT TTCAGTCAAA CAAATAGTAT TCAGACAGAC
    CTCTTTCCAA GTACAAAGCC TCAAGAACAG CAGAAAAGTC CGGTGGTGAA AGATGAAGCA
    CGCTCACTTT CACACTCTGT GGCCGTCACA TATTCTTTAT TCCAAGAAAA GAACATGTCT
    TTGAAAAGCA TATCTGAGAA CAGGACTCTG CCTCTCACAG CAGTTGGCGC ACACTTACTC
    CAAGCGCTGA AAGCCGGCTA CCCACTCGAC ATGGAGCGAG CAGGCCTGAC TCCAGAGACT
    CACAAGATTA TTGCTGATGT CATCCGAAAC CCTCCCGTCA ACTCAGATAT CAATAAAACT
    AAACTAATTA GAATGTTTGT TCCTGAAAAC ATTGACACAT ACCTTATCTA CATGGCAATT
    GAGATCCTGA GAAAAGATTG TGACAACAGA CCGTTATGCC AACCTTCATG TGATTCCAGT
    AAAAAGAGAT GTTTCCCCAA CTCTGAGGAC AGCTGTTCAC GTTCGAAGAG AAGCAAGGAC
    GAAGTAGGCA CTAATACCAA GGTTGATGGA AAACATCCTT GTGATCTTTC ACCAAAAGAT
    AAGAGTCACA GAAATTCACT GAGACTGACA TCTTGGAATC AGCCATCCAG CGATCCAGAA
    ATAGAAGATT TATTTACAGA CTCTCATCCA CAGACTTCAT CTGGAGCCTT AAAGAGAATG
    TATGCGGGGT TTGGCAAGAG AGATAAGATC TTAGTTGATA CCAGCAAAAA ATCAATGGCC
    AGAACGAAAA AGAAGGGTCT TTTTAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014592071.2
    CDS215..4564
    Translation

    Target ORF information:

    RefSeq Version XM_014736585.2
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014736585.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGGCTA ACTTGGAGAA GATGTATTCT
    TATAAAGCAT TTGATAAAAT TTCTTCTGGG AGTAACATTC CTAAAAAAAG CGTCATGTTG
    GAATCACCAA GAAAATCTTA CAAGTCCTCA GAACCTGTTA TTTCAGCTCA AGAGCAAGAG
    TCTCAGACTC TGTTATACGG TGAATTGGTA GAAGCTAGGC AGAAACGTGC CCATAACATG
    GACGTTCCTC CAGCTATCCT GGCAACAAAT AAGATACTGT TGGATATGGC CAAAATGAGA
    CCTACTACGG TTGAAAATGT GAAAAGGATC GACGGTGTTT CTGAAGGCAA AGCTACTATG
    TTGGCCCCTC TGTTGGAAGT CATCAAACAT TTCAGTCAAA CAAATAGTAT TCAGACAGAC
    CTCTTTCCAA GTACAAAGCC TCAAGAACAG CAGAAAAGTC CGGTGGTGAA AGATGAAGCA
    CGCTCACTTT CACACTCTGT GGCCGTCACA TATTCTTTAT TCCAAGAAAA GAACATGTCT
    TTGAAAAGCA TATCTGAGAA CAGGACTCTG CCTCTCACAG CAGTTGGCGC ACACTTACTC
    CAAGCGCTGA AAGCCGGCTA CCCACTCGAC ATGGAGCGAG CAGGCCTGAC TCCAGAGACT
    CACAAGATTA TTGCTGATGT CATCCGAAAC CCTCCCGTCA ACTCAGATAT CAATAAAACT
    AAACTAATTA GAATGTTTGT TCCTGAAAAC ATTGACACAT ACCTTATCTA CATGGCAATT
    GAGATCCTGA GAAAAGATTG TGACAACAGA CCGTTATGCC AACCTTCATG TGATTCCAGT
    AAAAAGAGAT GTTTCCCCAA CTCTGAGGAC AGCTGTTCAC GTTCGAAGAG AAGCAAGGAC
    GAAGTAGGCA CTAATACCAA GGTTGATGGA AAACATCCTT GTGATCTTTC ACCAAAAGAT
    AAGAGTCACA GAAATTCACT GAGACTGACA TCTTGGAATC AGCCATCCAG CGATCCAGAA
    ATAGAAGATT TATTTACAGA CTCTCATCCA CAGACTTCAT CTGGAGCCTT AAAGAGAATG
    TATGCGGGGT TTGGCAAGAG AGATAAGATC TTAGTTGATA CCAGCAAAAA ATCAATGGCC
    AGAACGAAAA AGAAGGGTCT TTTTAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60384
    Clone ID Related Accession (Same CDS sequence) XM_023630596.1
    Accession Version XM_023630596.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTTG ATGGAAAACA TCCTTGTGAT CTTTCACCAA AAGATAAGAG TCACAGAAAT
    TCACTGAGAC TGACATCTTG GAATCAGCCA TCCAGCGATC CAGAAATAGA AGATTTATTT
    ACAGACTCTC ATCCACAGAC TTCATCTGGA GCCTTAAAGA GAATGTATGC GGGGTTTGGC
    AAGAGAGATA AGATCTTAGT TGATACCAGC AAAAAATCAA TGGCCAGAAC GAAAAAGAAG
    GGTCTTTTTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486364.1
    CDS215..4549
    Translation

    Target ORF information:

    RefSeq Version XM_023630596.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630596.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGAAA AACAGTTGAA AACATCTCCA CCGAAGCGGA GACTTCCTGA ATGGATGTCT 
    ATGCCTAGTA AGAGATGTGC TGTAGAAGAA AGAAAGGCAT CTAGTCAGAA GAGTGTTTTT
    GAAGATGACC TTCCCTTCTT AGAATTCACT GGATCCATTG TGTACAGTTA TGAAGCTAGT
    GATTGTTCTT TCCTATCAGA AGATATTAGC TTGAGTCTGT CTGATGGAGA TGTGGTGGGA
    TTTGACATGG AATGGCCACC AATATATAAT AAACGGAAAC TGGACAGAGT TGCACTAATT
    CAGTTGTGTG TGTCAGAGAG CAAATGTTAC TTGTTTCATA TTTCTTCAAT GTCAGTTTTT
    CCTCAGGGAT TAAAAATGTT GCTTGAAAAC AAAGCAATTA AAAAAGTAGG TGTAGGAATT
    GAAGGGGATC AGTGGAAACT TCTACGTGAC TTTGACATCA AATTGAAGAG TTTTGTGGAG
    CTGACAGATG TTGCAAATGA AAAGCTGAAA TGTGCAGAGA TCTGGAGCCT CAATGGTTTG
    GTTAAACACC TCTTCGGTAA ACAGCTTCTG AAAGACAAGT CTGTCCGCTG TAGTGATTGG
    AATAATTTTC CTCTCACTGA GGATCAGAAA CTGTATGCAG CCACGGATGC TTATGCTGGT
    TTCATTATTT ACCGAAAATT AGAAATTTTG GGTGATGCTG TGCAAATGTT TACTATAAAT
    AGAGAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTTG ATGGAAAACA TCCTTGTGAT CTTTCACCAA AAGATAAGAG TCACAGAAAT
    TCACTGAGAC TGACATCTTG GAATCAGCCA TCCAGCGATC CAGAAATAGA AGATTTATTT
    ACAGACTCTC ATCCACAGAC TTCATCTGGA GCCTTAAAGA GAATGTATGC GGGGTTTGGC
    AAGAGAGATA AGATCTTAGT TGATACCAGC AAAAAATCAA TGGCCAGAAC GAAAAAGAAG
    GGTCTTTTTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60385
    Clone ID Related Accession (Same CDS sequence) XM_023630597.1
    Accession Version XM_023630597.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGCTGTA GAAGAAAGAA AGGTATGCAG TTCGCCAATC AGAAGAGTGT TTTTGAAGAT 
    GACCTTCCCT TCTTAGAATT CACTGGATCC ATTGTGTACA GTTATGAAGC TAGTGATTGT
    TCTTTCCTAT CAGAAGATAT TAGCTTGAGT CTGTCTGATG GAGATGTGGT GGGATTTGAC
    ATGGAATGGC CACCAATATA TAATAAACGG AAACTGGACA GAGTTGCACT AATTCAGTTG
    TGTGTGTCAG AGAGCAAATG TTACTTGTTT CATATTTCTT CAATGTCAGT TTTTCCTCAG
    GGATTAAAAA TGTTGCTTGA AAACAAAGCA ATTAAAAAAG TAGGTGTAGG AATTGAAGGG
    GATCAGTGGA AACTTCTACG TGACTTTGAC ATCAAATTGA AGAGTTTTGT GGAGCTGACA
    GATGTTGCAA ATGAAAAGCT GAAATGTGCA GAGATCTGGA GCCTCAATGG TTTGGTTAAA
    CACCTCTTCG GTAAACAGCT TCTGAAAGAC AAGTCTGTCC GCTGTAGTGA TTGGAATAAT
    TTTCCTCTCA CTGAGGATCA GAAACTGTAT GCAGCCACGG ATGCTTATGC TGGTTTCATT
    ATTTACCGAA AATTAGAAAT TTTGGGTGAT GCTGTGCAAA TGTTTACTAT AAATAGAGAG
    GAGGAACTTC TTTTTGGTGA CGTGAGGAAA CAGTTGACTT CAGTCTCCGA GGAGATGATG
    GATCTGGCTA AGCGTCTTCC TGACACTTTC AGAAAGTTGG AAAATCCACA GAGGGTTTCT
    GTACTGTTGA AGGATATTTC AGAAAATCTC GATTCATTGA GGAAGATGAT ATTTGGTTCT
    ACCGAGACCG AATTGAAGCC TAGCGGCAAT TCTAACTTAC TACCATTGGG AGACCCGACT
    GCTGGTGGAG TACTACAGAA ACAAATTAGA GAACATAAAA TTTTTACTGA AGTTAAAGAT
    GAGACATGGG ACACAACACT GGATAATTTC ATTAAACATG ATGGAGAAAA TGTACTTGGA
    AATGAAGTGA AAAGAAAAGA CGATGAATTT GAAGATGAAC TAGAAGCCAA TAAGTTGAAA
    GAGGAAACAG AAAGAATTAT GTCATTAGAT ATCACAGAAT ATGAACTCCA AATTTTGGAA
    CAGCAGGCTC AGGAAAAAAA TCTTAGTGAT GTTACTTACA AATCTCCTGA GCATGTATCC
    TCCAAGGATA ATGAAGAGGA TGTATCTTAT ATAATTGAAA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGACTTTAGA AAACCTAAAT ACTGTCACGG TAGATCCAAT TCATTCAAAA
    GGCACAGAAA TGGAAGGAAA TCTGGATGTT CCTTTTGAAG ATGAGGAAGA TGAGAGTGAA
    GCTGTTGAAG AGGAAGAAGA AGATGATGAA GACCGTTTGT TACCAGGACC TAATGCAGAG
    CAAGTGATTT GCCTCAAGAC CTACTTTGGC CATTCCAGTT TTAAGCCGGT TCAGTGGAAA
    GTGATTCATT CTGTATTGGA AGAAAGAAGA GATAACGTTG TTGTCATGGC AACTGGATAT
    GGAAAGAGTT TATGCTTCCA GTATCCACCA GTTTATGTAG GTGGGATTGC CATTGTCATC
    TCTCCTCTTA TTTCTTTGAT GGAAGACCAA GTGCTCCAGC TTGAAATGTC CAACATTCCA
    GCTTGTTTTC TGGGATCAGC ACAGTCAAAA AATGTTCTAG AGGATGTTAA ATTAGGCAAA
    TATCGGATTG TATACATAAC TCCAGAATTC TGTTCGAGGA ACTTGTACCT ACTCCACCAA
    CTTCAGGCTA ATATTGGTAT CACACTTATT GCTGTGGATG AGGCTCACTG TATTTCTGAG
    TGGGGACATG ATTTTAGAAA TTCATTCAGG AATTTGGGCT CCCTAAAAAC AGCATTCCCG
    TTGGTTCCAG TGGTCGCCCT CACTGCTACG GCCAGTTCTT CAATCCGGGA CGACATTGTG
    CGTTGCTTAG AACTGAAGAA CCCTCGGATC ACCTGTACTG GTTTTGATCG ACCGAACTTG
    TATTTAGAAG TTGGACAAAA AACAGGAAAC ATCCTTCAGG ATCTGAAGCA ATTTCTTGTC
    CAAAAGACAT GTTCTGCCTG GGAATTTGAA GGTCCAACTA TCATCTATTG TCTTTCCAGA
    AAAATGACAG AACAAGTTAC AGCTGAACTC AGGAAACTGC AGTTAGCCTG TGGAACATAC
    CATGCAGGCA TGGGTAGTAA CTCAAGGAAA GAGGTTCATC ATCGGTTCAT GAGAGATGAA
    ATTCAGTGTG TCGTAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGATATTCGC
    AAAGTCATTC ATTACGGTGC TCCTAAGGAA ATGGAATCAT ATTACCAGGA GATTGGTAGA
    GCTGGCCGGG ATGGACTTCA AAGTTCTTGT CACCTACTCT GGACTCCAAC AGACATCAAC
    TTAAATAGGA ACCGCCTTAG TGACATACCT GATGAAAAGT TTCGATTATA CAAATTAAAG
    ATGATGGAAA AGATGGAAAA ATATCTTCGT TCCAGCAAGT GTAGGCGACA AATCATCTTG
    TCTCATTTTG AAGATAAACA ACTACGAAAG GCCTCCGTGG GCATTATGGG AACTGAAAAT
    TGCTGTGATA ATTGCAGGTT CAGATTGGAT CATTGCTTTT TCATTGATGA CTCAGATGAT
    GCAGCCCAGG ACTTCGGTCC GCAAGCACTC CAGCTATTAT CTGCTGTGAA CATCTTAGGA
    GAAAAGTTTG GAATTGGGGT TCCAATTTTA TTTCTCCAAG GATCCAATTC CAAGCGTCTC
    GCAGATAAAT ATCGCAGTCA CAGTTTCTTT GGCACTGGCA AGGACCAAAC AAGGGCGTGG
    TGGAGGGCTC TTTCCCGTCA GCTCATAACT GAGGGATTCT TGGTCGAAGT GCCTGGGAAA
    GAAAAGTTTA TAAAGATTTG CACCCTTACA AAAAAGGGTA GAAATTGGCT TGATAAAGCC
    AAAAGAGGAT CCCCTCAGAG GCTAATCCTT GAAGCTAATG AAGAGCTGCG TCCAAGGAGG
    TTTCTTCTAC CGAGTTCTAA AACTGTATCT TCAGACACTG GACAGCATTC CTCTAGTCAA
    GTACCAACTG AATTAACTGC AGAGAATAAG CAGGCTAACT TGGAGAAGAT GTATTCTTAT
    AAAGCATTTG ATAAAATTTC TTCTGGGAGT AACATTCCTA AAAAAAGCGT CATGTTGGAA
    TCACCAAGAA AATCTTACAA GTCCTCAGAA CCTGTTATTT CAGCTCAAGA GCAAGAGTCT
    CAGACTCTGT TATACGGTGA ATTGGTAGAA GCTAGGCAGA AACGTGCCCA TAACATGGAC
    GTTCCTCCAG CTATCCTGGC AACAAATAAG ATACTGTTGG ATATGGCCAA AATGAGACCT
    ACTACGGTTG AAAATGTGAA AAGGATCGAC GGTGTTTCTG AAGGCAAAGC TACTATGTTG
    GCCCCTCTGT TGGAAGTCAT CAAACATTTC AGTCAAACAA ATAGTATTCA GACAGACCTC
    TTTCCAAGTA CAAAGCCTCA AGAACAGCAG AAAAGTCCGG TGGTGAAAGA TGAAGCACGC
    TCACTTTCAC ACTCTGTGGC CGTCACATAT TCTTTATTCC AAGAAAAGAA CATGTCTTTG
    AAAAGCATAT CTGAGAACAG GACTCTGCCT CTCACAGCAG TTGGCGCACA CTTACTCCAA
    GCGCTGAAAG CCGGCTACCC ACTCGACATG GAGCGAGCAG GCCTGACTCC AGAGACTCAC
    AAGATTATTG CTGATGTCAT CCGAAACCCT CCCGTCAACT CAGATATCAA TAAAACTAAA
    CTAATTAGAA TGTTTGTTCC TGAAAACATT GACACATACC TTATCTACAT GGCAATTGAG
    ATCCTGAGAA AAGATTGTGA CAACAGACCG TTATGCCAAC CTTCATGTGA TTCCAGTAAA
    AAGAGATGTT TCCCCAACTC TGAGGACAGC TGTTCACGTT CGAAGAGAAG CAAGGACGAA
    GTAGGCACTA ATACCAAGGT TGATGGAAAA CATCCTTGTG ATCTTTCACC AAAAGATAAG
    AGTCACAGAA ATTCACTGAG ACTGACATCT TGGAATCAGC CATCCAGCGA TCCAGAAATA
    GAAGATTTAT TTACAGACTC TCATCCACAG ACTTCATCTG GAGCCTTAAA GAGAATGTAT
    GCGGGGTTTG GCAAGAGAGA TAAGATCTTA GTTGATACCA GCAAAAAATC AATGGCCAGA
    ACGAAAAAGA AGGGTCTTTT TAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486365.1
    CDS87..4373
    Translation

    Target ORF information:

    RefSeq Version XM_023630597.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTGCTGTA GAAGAAAGAA AGGTATGCAG TTCGCCAATC AGAAGAGTGT TTTTGAAGAT 
    GACCTTCCCT TCTTAGAATT CACTGGATCC ATTGTGTACA GTTATGAAGC TAGTGATTGT
    TCTTTCCTAT CAGAAGATAT TAGCTTGAGT CTGTCTGATG GAGATGTGGT GGGATTTGAC
    ATGGAATGGC CACCAATATA TAATAAACGG AAACTGGACA GAGTTGCACT AATTCAGTTG
    TGTGTGTCAG AGAGCAAATG TTACTTGTTT CATATTTCTT CAATGTCAGT TTTTCCTCAG
    GGATTAAAAA TGTTGCTTGA AAACAAAGCA ATTAAAAAAG TAGGTGTAGG AATTGAAGGG
    GATCAGTGGA AACTTCTACG TGACTTTGAC ATCAAATTGA AGAGTTTTGT GGAGCTGACA
    GATGTTGCAA ATGAAAAGCT GAAATGTGCA GAGATCTGGA GCCTCAATGG TTTGGTTAAA
    CACCTCTTCG GTAAACAGCT TCTGAAAGAC AAGTCTGTCC GCTGTAGTGA TTGGAATAAT
    TTTCCTCTCA CTGAGGATCA GAAACTGTAT GCAGCCACGG ATGCTTATGC TGGTTTCATT
    ATTTACCGAA AATTAGAAAT TTTGGGTGAT GCTGTGCAAA TGTTTACTAT AAATAGAGAG
    GAGGAACTTC TTTTTGGTGA CGTGAGGAAA CAGTTGACTT CAGTCTCCGA GGAGATGATG
    GATCTGGCTA AGCGTCTTCC TGACACTTTC AGAAAGTTGG AAAATCCACA GAGGGTTTCT
    GTACTGTTGA AGGATATTTC AGAAAATCTC GATTCATTGA GGAAGATGAT ATTTGGTTCT
    ACCGAGACCG AATTGAAGCC TAGCGGCAAT TCTAACTTAC TACCATTGGG AGACCCGACT
    GCTGGTGGAG TACTACAGAA ACAAATTAGA GAACATAAAA TTTTTACTGA AGTTAAAGAT
    GAGACATGGG ACACAACACT GGATAATTTC ATTAAACATG ATGGAGAAAA TGTACTTGGA
    AATGAAGTGA AAAGAAAAGA CGATGAATTT GAAGATGAAC TAGAAGCCAA TAAGTTGAAA
    GAGGAAACAG AAAGAATTAT GTCATTAGAT ATCACAGAAT ATGAACTCCA AATTTTGGAA
    CAGCAGGCTC AGGAAAAAAA TCTTAGTGAT GTTACTTACA AATCTCCTGA GCATGTATCC
    TCCAAGGATA ATGAAGAGGA TGTATCTTAT ATAATTGAAA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGACTTTAGA AAACCTAAAT ACTGTCACGG TAGATCCAAT TCATTCAAAA
    GGCACAGAAA TGGAAGGAAA TCTGGATGTT CCTTTTGAAG ATGAGGAAGA TGAGAGTGAA
    GCTGTTGAAG AGGAAGAAGA AGATGATGAA GACCGTTTGT TACCAGGACC TAATGCAGAG
    CAAGTGATTT GCCTCAAGAC CTACTTTGGC CATTCCAGTT TTAAGCCGGT TCAGTGGAAA
    GTGATTCATT CTGTATTGGA AGAAAGAAGA GATAACGTTG TTGTCATGGC AACTGGATAT
    GGAAAGAGTT TATGCTTCCA GTATCCACCA GTTTATGTAG GTGGGATTGC CATTGTCATC
    TCTCCTCTTA TTTCTTTGAT GGAAGACCAA GTGCTCCAGC TTGAAATGTC CAACATTCCA
    GCTTGTTTTC TGGGATCAGC ACAGTCAAAA AATGTTCTAG AGGATGTTAA ATTAGGCAAA
    TATCGGATTG TATACATAAC TCCAGAATTC TGTTCGAGGA ACTTGTACCT ACTCCACCAA
    CTTCAGGCTA ATATTGGTAT CACACTTATT GCTGTGGATG AGGCTCACTG TATTTCTGAG
    TGGGGACATG ATTTTAGAAA TTCATTCAGG AATTTGGGCT CCCTAAAAAC AGCATTCCCG
    TTGGTTCCAG TGGTCGCCCT CACTGCTACG GCCAGTTCTT CAATCCGGGA CGACATTGTG
    CGTTGCTTAG AACTGAAGAA CCCTCGGATC ACCTGTACTG GTTTTGATCG ACCGAACTTG
    TATTTAGAAG TTGGACAAAA AACAGGAAAC ATCCTTCAGG ATCTGAAGCA ATTTCTTGTC
    CAAAAGACAT GTTCTGCCTG GGAATTTGAA GGTCCAACTA TCATCTATTG TCTTTCCAGA
    AAAATGACAG AACAAGTTAC AGCTGAACTC AGGAAACTGC AGTTAGCCTG TGGAACATAC
    CATGCAGGCA TGGGTAGTAA CTCAAGGAAA GAGGTTCATC ATCGGTTCAT GAGAGATGAA
    ATTCAGTGTG TCGTAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGATATTCGC
    AAAGTCATTC ATTACGGTGC TCCTAAGGAA ATGGAATCAT ATTACCAGGA GATTGGTAGA
    GCTGGCCGGG ATGGACTTCA AAGTTCTTGT CACCTACTCT GGACTCCAAC AGACATCAAC
    TTAAATAGGA ACCGCCTTAG TGACATACCT GATGAAAAGT TTCGATTATA CAAATTAAAG
    ATGATGGAAA AGATGGAAAA ATATCTTCGT TCCAGCAAGT GTAGGCGACA AATCATCTTG
    TCTCATTTTG AAGATAAACA ACTACGAAAG GCCTCCGTGG GCATTATGGG AACTGAAAAT
    TGCTGTGATA ATTGCAGGTT CAGATTGGAT CATTGCTTTT TCATTGATGA CTCAGATGAT
    GCAGCCCAGG ACTTCGGTCC GCAAGCACTC CAGCTATTAT CTGCTGTGAA CATCTTAGGA
    GAAAAGTTTG GAATTGGGGT TCCAATTTTA TTTCTCCAAG GATCCAATTC CAAGCGTCTC
    GCAGATAAAT ATCGCAGTCA CAGTTTCTTT GGCACTGGCA AGGACCAAAC AAGGGCGTGG
    TGGAGGGCTC TTTCCCGTCA GCTCATAACT GAGGGATTCT TGGTCGAAGT GCCTGGGAAA
    GAAAAGTTTA TAAAGATTTG CACCCTTACA AAAAAGGGTA GAAATTGGCT TGATAAAGCC
    AAAAGAGGAT CCCCTCAGAG GCTAATCCTT GAAGCTAATG AAGAGCTGCG TCCAAGGAGG
    TTTCTTCTAC CGAGTTCTAA AACTGTATCT TCAGACACTG GACAGCATTC CTCTAGTCAA
    GTACCAACTG AATTAACTGC AGAGAATAAG CAGGCTAACT TGGAGAAGAT GTATTCTTAT
    AAAGCATTTG ATAAAATTTC TTCTGGGAGT AACATTCCTA AAAAAAGCGT CATGTTGGAA
    TCACCAAGAA AATCTTACAA GTCCTCAGAA CCTGTTATTT CAGCTCAAGA GCAAGAGTCT
    CAGACTCTGT TATACGGTGA ATTGGTAGAA GCTAGGCAGA AACGTGCCCA TAACATGGAC
    GTTCCTCCAG CTATCCTGGC AACAAATAAG ATACTGTTGG ATATGGCCAA AATGAGACCT
    ACTACGGTTG AAAATGTGAA AAGGATCGAC GGTGTTTCTG AAGGCAAAGC TACTATGTTG
    GCCCCTCTGT TGGAAGTCAT CAAACATTTC AGTCAAACAA ATAGTATTCA GACAGACCTC
    TTTCCAAGTA CAAAGCCTCA AGAACAGCAG AAAAGTCCGG TGGTGAAAGA TGAAGCACGC
    TCACTTTCAC ACTCTGTGGC CGTCACATAT TCTTTATTCC AAGAAAAGAA CATGTCTTTG
    AAAAGCATAT CTGAGAACAG GACTCTGCCT CTCACAGCAG TTGGCGCACA CTTACTCCAA
    GCGCTGAAAG CCGGCTACCC ACTCGACATG GAGCGAGCAG GCCTGACTCC AGAGACTCAC
    AAGATTATTG CTGATGTCAT CCGAAACCCT CCCGTCAACT CAGATATCAA TAAAACTAAA
    CTAATTAGAA TGTTTGTTCC TGAAAACATT GACACATACC TTATCTACAT GGCAATTGAG
    ATCCTGAGAA AAGATTGTGA CAACAGACCG TTATGCCAAC CTTCATGTGA TTCCAGTAAA
    AAGAGATGTT TCCCCAACTC TGAGGACAGC TGTTCACGTT CGAAGAGAAG CAAGGACGAA
    GTAGGCACTA ATACCAAGGT TGATGGAAAA CATCCTTGTG ATCTTTCACC AAAAGATAAG
    AGTCACAGAA ATTCACTGAG ACTGACATCT TGGAATCAGC CATCCAGCGA TCCAGAAATA
    GAAGATTTAT TTACAGACTC TCATCCACAG ACTTCATCTG GAGCCTTAAA GAGAATGTAT
    GCGGGGTTTG GCAAGAGAGA TAAGATCTTA GTTGATACCA GCAAAAAATC AATGGCCAGA
    ACGAAAAAGA AGGGTCTTTT TAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60386
    Clone ID Related Accession (Same CDS sequence) XM_023630598.1 , XM_023630599.1
    Accession Version XM_023630598.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAATGGC CACCAATATA TAATAAACGG AAACTGGACA GAGTTGCACT AATTCAGTTG 
    TGTGTGTCAG AGAGCAAATG TTACTTGTTT CATATTTCTT CAATGTCAGT TTTTCCTCAG
    GGATTAAAAA TGTTGCTTGA AAACAAAGCA ATTAAAAAAG TAGGTGTAGG AATTGAAGGG
    GATCAGTGGA AACTTCTACG TGACTTTGAC ATCAAATTGA AGAGTTTTGT GGAGCTGACA
    GATGTTGCAA ATGAAAAGCT GAAATGTGCA GAGATCTGGA GCCTCAATGG TTTGGTTAAA
    CACCTCTTCG GTAAACAGCT TCTGAAAGAC AAGTCTGTCC GCTGTAGTGA TTGGAATAAT
    TTTCCTCTCA CTGAGGATCA GAAACTGTAT GCAGCCACGG ATGCTTATGC TGGTTTCATT
    ATTTACCGAA AATTAGAAAT TTTGGGTGAT GCTGTGCAAA TGTTTACTAT AAATAGAGAG
    GAGGAACTTC TTTTTGGTGA CGTGAGGAAA CAGTTGACTT CAGTCTCCGA GGAGATGATG
    GATCTGGCTA AGCGTCTTCC TGACACTTTC AGAAAGTTGG AAAATCCACA GAGGGTTTCT
    GTACTGTTGA AGGATATTTC AGAAAATCTC GATTCATTGA GGAAGATGAT ATTTGGTTCT
    ACCGAGACCG AATTGAAGCC TAGCGGCAAT TCTAACTTAC TACCATTGGG AGACCCGACT
    GCTGGTGGAG TACTACAGAA ACAAATTAGA GAACATAAAA TTTTTACTGA AGTTAAAGAT
    GAGACATGGG ACACAACACT GGATAATTTC ATTAAACATG ATGGAGAAAA TGTACTTGGA
    AATGAAGTGA AAAGAAAAGA CGATGAATTT GAAGATGAAC TAGAAGCCAA TAAGTTGAAA
    GAGGAAACAG AAAGAATTAT GTCATTAGAT ATCACAGAAT ATGAACTCCA AATTTTGGAA
    CAGCAGGCTC AGGAAAAAAA TCTTAGTGAT GTTACTTACA AATCTCCTGA GCATGTATCC
    TCCAAGGATA ATGAAGAGGA TGTATCTTAT ATAATTGAAA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGACTTTAGA AAACCTAAAT ACTGTCACGG TAGATCCAAT TCATTCAAAA
    GGCACAGAAA TGGAAGGAAA TCTGGATGTT CCTTTTGAAG ATGAGGAAGA TGAGAGTGAA
    GCTGTTGAAG AGGAAGAAGA AGATGATGAA GACCGTTTGT TACCAGGACC TAATGCAGAG
    CAAGTGATTT GCCTCAAGAC CTACTTTGGC CATTCCAGTT TTAAGCCGGT TCAGTGGAAA
    GTGATTCATT CTGTATTGGA AGAAAGAAGA GATAACGTTG TTGTCATGGC AACTGGATAT
    GGAAAGAGTT TATGCTTCCA GTATCCACCA GTTTATGTAG GTGGGATTGC CATTGTCATC
    TCTCCTCTTA TTTCTTTGAT GGAAGACCAA GTGCTCCAGC TTGAAATGTC CAACATTCCA
    GCTTGTTTTC TGGGATCAGC ACAGTCAAAA AATGTTCTAG AGGATGTTAA ATTAGGCAAA
    TATCGGATTG TATACATAAC TCCAGAATTC TGTTCGAGGA ACTTGTACCT ACTCCACCAA
    CTTCAGGCTA ATATTGGTAT CACACTTATT GCTGTGGATG AGGCTCACTG TATTTCTGAG
    TGGGGACATG ATTTTAGAAA TTCATTCAGG AATTTGGGCT CCCTAAAAAC AGCATTCCCG
    TTGGTTCCAG TGGTCGCCCT CACTGCTACG GCCAGTTCTT CAATCCGGGA CGACATTGTG
    CGTTGCTTAG AACTGAAGAA CCCTCGGATC ACCTGTACTG GTTTTGATCG ACCGAACTTG
    TATTTAGAAG TTGGACAAAA AACAGGAAAC ATCCTTCAGG ATCTGAAGCA ATTTCTTGTC
    CAAAAGACAT GTTCTGCCTG GGAATTTGAA GGTCCAACTA TCATCTATTG TCTTTCCAGA
    AAAATGACAG AACAAGTTAC AGCTGAACTC AGGAAACTGC AGTTAGCCTG TGGAACATAC
    CATGCAGGCA TGGGTAGTAA CTCAAGGAAA GAGGTTCATC ATCGGTTCAT GAGAGATGAA
    ATTCAGTGTG TCGTAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGATATTCGC
    AAAGTCATTC ATTACGGTGC TCCTAAGGAA ATGGAATCAT ATTACCAGGA GATTGGTAGA
    GCTGGCCGGG ATGGACTTCA AAGTTCTTGT CACCTACTCT GGACTCCAAC AGACATCAAC
    TTAAATAGGA ACCGCCTTAG TGACATACCT GATGAAAAGT TTCGATTATA CAAATTAAAG
    ATGATGGAAA AGATGGAAAA ATATCTTCGT TCCAGCAAGT GTAGGCGACA AATCATCTTG
    TCTCATTTTG AAGATAAACA ACTACGAAAG GCCTCCGTGG GCATTATGGG AACTGAAAAT
    TGCTGTGATA ATTGCAGGTT CAGATTGGAT CATTGCTTTT TCATTGATGA CTCAGATGAT
    GCAGCCCAGG ACTTCGGTCC GCAAGCACTC CAGCTATTAT CTGCTGTGAA CATCTTAGGA
    GAAAAGTTTG GAATTGGGGT TCCAATTTTA TTTCTCCAAG GATCCAATTC CAAGCGTCTC
    GCAGATAAAT ATCGCAGTCA CAGTTTCTTT GGCACTGGCA AGGACCAAAC AAGGGCGTGG
    TGGAGGGCTC TTTCCCGTCA GCTCATAACT GAGGGATTCT TGGTCGAAGT GCCTGGGAAA
    GAAAAGTTTA TAAAGATTTG CACCCTTACA AAAAAGGGTA GAAATTGGCT TGATAAAGCC
    AAAAGAGGAT CCCCTCAGAG GCTAATCCTT GAAGCTAATG AAGAGCTGCG TCCAAGGAGG
    TTTCTTCTAC CGAGTTCTAA AACTGTATCT TCAGACACTG GACAGCATTC CTCTAGTCAA
    GTACCAACTG AATTAACTGC AGAGAATAAG CAGGCTAACT TGGAGAAGAT GTATTCTTAT
    AAAGCATTTG ATAAAATTTC TTCTGGGAGT AACATTCCTA AAAAAAGCGT CATGTTGGAA
    TCACCAAGAA AATCTTACAA GTCCTCAGAA CCTGTTATTT CAGCTCAAGA GCAAGAGTCT
    CAGACTCTGT TATACGGTGA ATTGGTAGAA GCTAGGCAGA AACGTGCCCA TAACATGGAC
    GTTCCTCCAG CTATCCTGGC AACAAATAAG ATACTGTTGG ATATGGCCAA AATGAGACCT
    ACTACGGTTG AAAATGTGAA AAGGATCGAC GGTGTTTCTG AAGGCAAAGC TACTATGTTG
    GCCCCTCTGT TGGAAGTCAT CAAACATTTC AGTCAAACAA ATAGTATTCA GACAGACCTC
    TTTCCAAGTA CAAAGCCTCA AGAACAGCAG AAAAGTCCGG TGGTGAAAGA TGAAGCACGC
    TCACTTTCAC ACTCTGTGGC CGTCACATAT TCTTTATTCC AAGAAAAGAA CATGTCTTTG
    AAAAGCATAT CTGAGAACAG GACTCTGCCT CTCACAGCAG TTGGCGCACA CTTACTCCAA
    GCGCTGAAAG CCGGCTACCC ACTCGACATG GAGCGAGCAG GCCTGACTCC AGAGACTCAC
    AAGATTATTG CTGATGTCAT CCGAAACCCT CCCGTCAACT CAGATATCAA TAAAACTAAA
    CTAATTAGAA TGTTTGTTCC TGAAAACATT GACACATACC TTATCTACAT GGCAATTGAG
    ATCCTGAGAA AAGATTGTGA CAACAGACCG TTATGCCAAC CTTCATGTGA TTCCAGTAAA
    AAGAGATGTT TCCCCAACTC TGAGGACAGC TGTTCACGTT CGAAGAGAAG CAAGGACGAA
    GTAGGCACTA ATACCAAGGT TGATGGAAAA CATCCTTGTG ATCTTTCACC AAAAGATAAG
    AGTCACAGAA ATTCACTGAG ACTGACATCT TGGAATCAGC CATCCAGCGA TCCAGAAATA
    GAAGATTTAT TTACAGACTC TCATCCACAG ACTTCATCTG GAGCCTTAAA GAGAATGTAT
    GCGGGGTTTG GCAAGAGAGA TAAGATCTTA GTTGATACCA GCAAAAAATC AATGGCCAGA
    ACGAAAAAGA AGGGTCTTTT TAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486366.1
    CDS548..4654
    Translation

    Target ORF information:

    RefSeq Version XM_023630598.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAATGGC CACCAATATA TAATAAACGG AAACTGGACA GAGTTGCACT AATTCAGTTG 
    TGTGTGTCAG AGAGCAAATG TTACTTGTTT CATATTTCTT CAATGTCAGT TTTTCCTCAG
    GGATTAAAAA TGTTGCTTGA AAACAAAGCA ATTAAAAAAG TAGGTGTAGG AATTGAAGGG
    GATCAGTGGA AACTTCTACG TGACTTTGAC ATCAAATTGA AGAGTTTTGT GGAGCTGACA
    GATGTTGCAA ATGAAAAGCT GAAATGTGCA GAGATCTGGA GCCTCAATGG TTTGGTTAAA
    CACCTCTTCG GTAAACAGCT TCTGAAAGAC AAGTCTGTCC GCTGTAGTGA TTGGAATAAT
    TTTCCTCTCA CTGAGGATCA GAAACTGTAT GCAGCCACGG ATGCTTATGC TGGTTTCATT
    ATTTACCGAA AATTAGAAAT TTTGGGTGAT GCTGTGCAAA TGTTTACTAT AAATAGAGAG
    GAGGAACTTC TTTTTGGTGA CGTGAGGAAA CAGTTGACTT CAGTCTCCGA GGAGATGATG
    GATCTGGCTA AGCGTCTTCC TGACACTTTC AGAAAGTTGG AAAATCCACA GAGGGTTTCT
    GTACTGTTGA AGGATATTTC AGAAAATCTC GATTCATTGA GGAAGATGAT ATTTGGTTCT
    ACCGAGACCG AATTGAAGCC TAGCGGCAAT TCTAACTTAC TACCATTGGG AGACCCGACT
    GCTGGTGGAG TACTACAGAA ACAAATTAGA GAACATAAAA TTTTTACTGA AGTTAAAGAT
    GAGACATGGG ACACAACACT GGATAATTTC ATTAAACATG ATGGAGAAAA TGTACTTGGA
    AATGAAGTGA AAAGAAAAGA CGATGAATTT GAAGATGAAC TAGAAGCCAA TAAGTTGAAA
    GAGGAAACAG AAAGAATTAT GTCATTAGAT ATCACAGAAT ATGAACTCCA AATTTTGGAA
    CAGCAGGCTC AGGAAAAAAA TCTTAGTGAT GTTACTTACA AATCTCCTGA GCATGTATCC
    TCCAAGGATA ATGAAGAGGA TGTATCTTAT ATAATTGAAA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGACTTTAGA AAACCTAAAT ACTGTCACGG TAGATCCAAT TCATTCAAAA
    GGCACAGAAA TGGAAGGAAA TCTGGATGTT CCTTTTGAAG ATGAGGAAGA TGAGAGTGAA
    GCTGTTGAAG AGGAAGAAGA AGATGATGAA GACCGTTTGT TACCAGGACC TAATGCAGAG
    CAAGTGATTT GCCTCAAGAC CTACTTTGGC CATTCCAGTT TTAAGCCGGT TCAGTGGAAA
    GTGATTCATT CTGTATTGGA AGAAAGAAGA GATAACGTTG TTGTCATGGC AACTGGATAT
    GGAAAGAGTT TATGCTTCCA GTATCCACCA GTTTATGTAG GTGGGATTGC CATTGTCATC
    TCTCCTCTTA TTTCTTTGAT GGAAGACCAA GTGCTCCAGC TTGAAATGTC CAACATTCCA
    GCTTGTTTTC TGGGATCAGC ACAGTCAAAA AATGTTCTAG AGGATGTTAA ATTAGGCAAA
    TATCGGATTG TATACATAAC TCCAGAATTC TGTTCGAGGA ACTTGTACCT ACTCCACCAA
    CTTCAGGCTA ATATTGGTAT CACACTTATT GCTGTGGATG AGGCTCACTG TATTTCTGAG
    TGGGGACATG ATTTTAGAAA TTCATTCAGG AATTTGGGCT CCCTAAAAAC AGCATTCCCG
    TTGGTTCCAG TGGTCGCCCT CACTGCTACG GCCAGTTCTT CAATCCGGGA CGACATTGTG
    CGTTGCTTAG AACTGAAGAA CCCTCGGATC ACCTGTACTG GTTTTGATCG ACCGAACTTG
    TATTTAGAAG TTGGACAAAA AACAGGAAAC ATCCTTCAGG ATCTGAAGCA ATTTCTTGTC
    CAAAAGACAT GTTCTGCCTG GGAATTTGAA GGTCCAACTA TCATCTATTG TCTTTCCAGA
    AAAATGACAG AACAAGTTAC AGCTGAACTC AGGAAACTGC AGTTAGCCTG TGGAACATAC
    CATGCAGGCA TGGGTAGTAA CTCAAGGAAA GAGGTTCATC ATCGGTTCAT GAGAGATGAA
    ATTCAGTGTG TCGTAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGATATTCGC
    AAAGTCATTC ATTACGGTGC TCCTAAGGAA ATGGAATCAT ATTACCAGGA GATTGGTAGA
    GCTGGCCGGG ATGGACTTCA AAGTTCTTGT CACCTACTCT GGACTCCAAC AGACATCAAC
    TTAAATAGGA ACCGCCTTAG TGACATACCT GATGAAAAGT TTCGATTATA CAAATTAAAG
    ATGATGGAAA AGATGGAAAA ATATCTTCGT TCCAGCAAGT GTAGGCGACA AATCATCTTG
    TCTCATTTTG AAGATAAACA ACTACGAAAG GCCTCCGTGG GCATTATGGG AACTGAAAAT
    TGCTGTGATA ATTGCAGGTT CAGATTGGAT CATTGCTTTT TCATTGATGA CTCAGATGAT
    GCAGCCCAGG ACTTCGGTCC GCAAGCACTC CAGCTATTAT CTGCTGTGAA CATCTTAGGA
    GAAAAGTTTG GAATTGGGGT TCCAATTTTA TTTCTCCAAG GATCCAATTC CAAGCGTCTC
    GCAGATAAAT ATCGCAGTCA CAGTTTCTTT GGCACTGGCA AGGACCAAAC AAGGGCGTGG
    TGGAGGGCTC TTTCCCGTCA GCTCATAACT GAGGGATTCT TGGTCGAAGT GCCTGGGAAA
    GAAAAGTTTA TAAAGATTTG CACCCTTACA AAAAAGGGTA GAAATTGGCT TGATAAAGCC
    AAAAGAGGAT CCCCTCAGAG GCTAATCCTT GAAGCTAATG AAGAGCTGCG TCCAAGGAGG
    TTTCTTCTAC CGAGTTCTAA AACTGTATCT TCAGACACTG GACAGCATTC CTCTAGTCAA
    GTACCAACTG AATTAACTGC AGAGAATAAG CAGGCTAACT TGGAGAAGAT GTATTCTTAT
    AAAGCATTTG ATAAAATTTC TTCTGGGAGT AACATTCCTA AAAAAAGCGT CATGTTGGAA
    TCACCAAGAA AATCTTACAA GTCCTCAGAA CCTGTTATTT CAGCTCAAGA GCAAGAGTCT
    CAGACTCTGT TATACGGTGA ATTGGTAGAA GCTAGGCAGA AACGTGCCCA TAACATGGAC
    GTTCCTCCAG CTATCCTGGC AACAAATAAG ATACTGTTGG ATATGGCCAA AATGAGACCT
    ACTACGGTTG AAAATGTGAA AAGGATCGAC GGTGTTTCTG AAGGCAAAGC TACTATGTTG
    GCCCCTCTGT TGGAAGTCAT CAAACATTTC AGTCAAACAA ATAGTATTCA GACAGACCTC
    TTTCCAAGTA CAAAGCCTCA AGAACAGCAG AAAAGTCCGG TGGTGAAAGA TGAAGCACGC
    TCACTTTCAC ACTCTGTGGC CGTCACATAT TCTTTATTCC AAGAAAAGAA CATGTCTTTG
    AAAAGCATAT CTGAGAACAG GACTCTGCCT CTCACAGCAG TTGGCGCACA CTTACTCCAA
    GCGCTGAAAG CCGGCTACCC ACTCGACATG GAGCGAGCAG GCCTGACTCC AGAGACTCAC
    AAGATTATTG CTGATGTCAT CCGAAACCCT CCCGTCAACT CAGATATCAA TAAAACTAAA
    CTAATTAGAA TGTTTGTTCC TGAAAACATT GACACATACC TTATCTACAT GGCAATTGAG
    ATCCTGAGAA AAGATTGTGA CAACAGACCG TTATGCCAAC CTTCATGTGA TTCCAGTAAA
    AAGAGATGTT TCCCCAACTC TGAGGACAGC TGTTCACGTT CGAAGAGAAG CAAGGACGAA
    GTAGGCACTA ATACCAAGGT TGATGGAAAA CATCCTTGTG ATCTTTCACC AAAAGATAAG
    AGTCACAGAA ATTCACTGAG ACTGACATCT TGGAATCAGC CATCCAGCGA TCCAGAAATA
    GAAGATTTAT TTACAGACTC TCATCCACAG ACTTCATCTG GAGCCTTAAA GAGAATGTAT
    GCGGGGTTTG GCAAGAGAGA TAAGATCTTA GTTGATACCA GCAAAAAATC AATGGCCAGA
    ACGAAAAAGA AGGGTCTTTT TAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60386
    Clone ID Related Accession (Same CDS sequence) XM_023630598.1 , XM_023630599.1
    Accession Version XM_023630599.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAATGGC CACCAATATA TAATAAACGG AAACTGGACA GAGTTGCACT AATTCAGTTG 
    TGTGTGTCAG AGAGCAAATG TTACTTGTTT CATATTTCTT CAATGTCAGT TTTTCCTCAG
    GGATTAAAAA TGTTGCTTGA AAACAAAGCA ATTAAAAAAG TAGGTGTAGG AATTGAAGGG
    GATCAGTGGA AACTTCTACG TGACTTTGAC ATCAAATTGA AGAGTTTTGT GGAGCTGACA
    GATGTTGCAA ATGAAAAGCT GAAATGTGCA GAGATCTGGA GCCTCAATGG TTTGGTTAAA
    CACCTCTTCG GTAAACAGCT TCTGAAAGAC AAGTCTGTCC GCTGTAGTGA TTGGAATAAT
    TTTCCTCTCA CTGAGGATCA GAAACTGTAT GCAGCCACGG ATGCTTATGC TGGTTTCATT
    ATTTACCGAA AATTAGAAAT TTTGGGTGAT GCTGTGCAAA TGTTTACTAT AAATAGAGAG
    GAGGAACTTC TTTTTGGTGA CGTGAGGAAA CAGTTGACTT CAGTCTCCGA GGAGATGATG
    GATCTGGCTA AGCGTCTTCC TGACACTTTC AGAAAGTTGG AAAATCCACA GAGGGTTTCT
    GTACTGTTGA AGGATATTTC AGAAAATCTC GATTCATTGA GGAAGATGAT ATTTGGTTCT
    ACCGAGACCG AATTGAAGCC TAGCGGCAAT TCTAACTTAC TACCATTGGG AGACCCGACT
    GCTGGTGGAG TACTACAGAA ACAAATTAGA GAACATAAAA TTTTTACTGA AGTTAAAGAT
    GAGACATGGG ACACAACACT GGATAATTTC ATTAAACATG ATGGAGAAAA TGTACTTGGA
    AATGAAGTGA AAAGAAAAGA CGATGAATTT GAAGATGAAC TAGAAGCCAA TAAGTTGAAA
    GAGGAAACAG AAAGAATTAT GTCATTAGAT ATCACAGAAT ATGAACTCCA AATTTTGGAA
    CAGCAGGCTC AGGAAAAAAA TCTTAGTGAT GTTACTTACA AATCTCCTGA GCATGTATCC
    TCCAAGGATA ATGAAGAGGA TGTATCTTAT ATAATTGAAA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGACTTTAGA AAACCTAAAT ACTGTCACGG TAGATCCAAT TCATTCAAAA
    GGCACAGAAA TGGAAGGAAA TCTGGATGTT CCTTTTGAAG ATGAGGAAGA TGAGAGTGAA
    GCTGTTGAAG AGGAAGAAGA AGATGATGAA GACCGTTTGT TACCAGGACC TAATGCAGAG
    CAAGTGATTT GCCTCAAGAC CTACTTTGGC CATTCCAGTT TTAAGCCGGT TCAGTGGAAA
    GTGATTCATT CTGTATTGGA AGAAAGAAGA GATAACGTTG TTGTCATGGC AACTGGATAT
    GGAAAGAGTT TATGCTTCCA GTATCCACCA GTTTATGTAG GTGGGATTGC CATTGTCATC
    TCTCCTCTTA TTTCTTTGAT GGAAGACCAA GTGCTCCAGC TTGAAATGTC CAACATTCCA
    GCTTGTTTTC TGGGATCAGC ACAGTCAAAA AATGTTCTAG AGGATGTTAA ATTAGGCAAA
    TATCGGATTG TATACATAAC TCCAGAATTC TGTTCGAGGA ACTTGTACCT ACTCCACCAA
    CTTCAGGCTA ATATTGGTAT CACACTTATT GCTGTGGATG AGGCTCACTG TATTTCTGAG
    TGGGGACATG ATTTTAGAAA TTCATTCAGG AATTTGGGCT CCCTAAAAAC AGCATTCCCG
    TTGGTTCCAG TGGTCGCCCT CACTGCTACG GCCAGTTCTT CAATCCGGGA CGACATTGTG
    CGTTGCTTAG AACTGAAGAA CCCTCGGATC ACCTGTACTG GTTTTGATCG ACCGAACTTG
    TATTTAGAAG TTGGACAAAA AACAGGAAAC ATCCTTCAGG ATCTGAAGCA ATTTCTTGTC
    CAAAAGACAT GTTCTGCCTG GGAATTTGAA GGTCCAACTA TCATCTATTG TCTTTCCAGA
    AAAATGACAG AACAAGTTAC AGCTGAACTC AGGAAACTGC AGTTAGCCTG TGGAACATAC
    CATGCAGGCA TGGGTAGTAA CTCAAGGAAA GAGGTTCATC ATCGGTTCAT GAGAGATGAA
    ATTCAGTGTG TCGTAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGATATTCGC
    AAAGTCATTC ATTACGGTGC TCCTAAGGAA ATGGAATCAT ATTACCAGGA GATTGGTAGA
    GCTGGCCGGG ATGGACTTCA AAGTTCTTGT CACCTACTCT GGACTCCAAC AGACATCAAC
    TTAAATAGGA ACCGCCTTAG TGACATACCT GATGAAAAGT TTCGATTATA CAAATTAAAG
    ATGATGGAAA AGATGGAAAA ATATCTTCGT TCCAGCAAGT GTAGGCGACA AATCATCTTG
    TCTCATTTTG AAGATAAACA ACTACGAAAG GCCTCCGTGG GCATTATGGG AACTGAAAAT
    TGCTGTGATA ATTGCAGGTT CAGATTGGAT CATTGCTTTT TCATTGATGA CTCAGATGAT
    GCAGCCCAGG ACTTCGGTCC GCAAGCACTC CAGCTATTAT CTGCTGTGAA CATCTTAGGA
    GAAAAGTTTG GAATTGGGGT TCCAATTTTA TTTCTCCAAG GATCCAATTC CAAGCGTCTC
    GCAGATAAAT ATCGCAGTCA CAGTTTCTTT GGCACTGGCA AGGACCAAAC AAGGGCGTGG
    TGGAGGGCTC TTTCCCGTCA GCTCATAACT GAGGGATTCT TGGTCGAAGT GCCTGGGAAA
    GAAAAGTTTA TAAAGATTTG CACCCTTACA AAAAAGGGTA GAAATTGGCT TGATAAAGCC
    AAAAGAGGAT CCCCTCAGAG GCTAATCCTT GAAGCTAATG AAGAGCTGCG TCCAAGGAGG
    TTTCTTCTAC CGAGTTCTAA AACTGTATCT TCAGACACTG GACAGCATTC CTCTAGTCAA
    GTACCAACTG AATTAACTGC AGAGAATAAG CAGGCTAACT TGGAGAAGAT GTATTCTTAT
    AAAGCATTTG ATAAAATTTC TTCTGGGAGT AACATTCCTA AAAAAAGCGT CATGTTGGAA
    TCACCAAGAA AATCTTACAA GTCCTCAGAA CCTGTTATTT CAGCTCAAGA GCAAGAGTCT
    CAGACTCTGT TATACGGTGA ATTGGTAGAA GCTAGGCAGA AACGTGCCCA TAACATGGAC
    GTTCCTCCAG CTATCCTGGC AACAAATAAG ATACTGTTGG ATATGGCCAA AATGAGACCT
    ACTACGGTTG AAAATGTGAA AAGGATCGAC GGTGTTTCTG AAGGCAAAGC TACTATGTTG
    GCCCCTCTGT TGGAAGTCAT CAAACATTTC AGTCAAACAA ATAGTATTCA GACAGACCTC
    TTTCCAAGTA CAAAGCCTCA AGAACAGCAG AAAAGTCCGG TGGTGAAAGA TGAAGCACGC
    TCACTTTCAC ACTCTGTGGC CGTCACATAT TCTTTATTCC AAGAAAAGAA CATGTCTTTG
    AAAAGCATAT CTGAGAACAG GACTCTGCCT CTCACAGCAG TTGGCGCACA CTTACTCCAA
    GCGCTGAAAG CCGGCTACCC ACTCGACATG GAGCGAGCAG GCCTGACTCC AGAGACTCAC
    AAGATTATTG CTGATGTCAT CCGAAACCCT CCCGTCAACT CAGATATCAA TAAAACTAAA
    CTAATTAGAA TGTTTGTTCC TGAAAACATT GACACATACC TTATCTACAT GGCAATTGAG
    ATCCTGAGAA AAGATTGTGA CAACAGACCG TTATGCCAAC CTTCATGTGA TTCCAGTAAA
    AAGAGATGTT TCCCCAACTC TGAGGACAGC TGTTCACGTT CGAAGAGAAG CAAGGACGAA
    GTAGGCACTA ATACCAAGGT TGATGGAAAA CATCCTTGTG ATCTTTCACC AAAAGATAAG
    AGTCACAGAA ATTCACTGAG ACTGACATCT TGGAATCAGC CATCCAGCGA TCCAGAAATA
    GAAGATTTAT TTACAGACTC TCATCCACAG ACTTCATCTG GAGCCTTAAA GAGAATGTAT
    GCGGGGTTTG GCAAGAGAGA TAAGATCTTA GTTGATACCA GCAAAAAATC AATGGCCAGA
    ACGAAAAAGA AGGGTCTTTT TAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486367.1
    CDS239..4345
    Translation

    Target ORF information:

    RefSeq Version XM_023630599.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAATGGC CACCAATATA TAATAAACGG AAACTGGACA GAGTTGCACT AATTCAGTTG 
    TGTGTGTCAG AGAGCAAATG TTACTTGTTT CATATTTCTT CAATGTCAGT TTTTCCTCAG
    GGATTAAAAA TGTTGCTTGA AAACAAAGCA ATTAAAAAAG TAGGTGTAGG AATTGAAGGG
    GATCAGTGGA AACTTCTACG TGACTTTGAC ATCAAATTGA AGAGTTTTGT GGAGCTGACA
    GATGTTGCAA ATGAAAAGCT GAAATGTGCA GAGATCTGGA GCCTCAATGG TTTGGTTAAA
    CACCTCTTCG GTAAACAGCT TCTGAAAGAC AAGTCTGTCC GCTGTAGTGA TTGGAATAAT
    TTTCCTCTCA CTGAGGATCA GAAACTGTAT GCAGCCACGG ATGCTTATGC TGGTTTCATT
    ATTTACCGAA AATTAGAAAT TTTGGGTGAT GCTGTGCAAA TGTTTACTAT AAATAGAGAG
    GAGGAACTTC TTTTTGGTGA CGTGAGGAAA CAGTTGACTT CAGTCTCCGA GGAGATGATG
    GATCTGGCTA AGCGTCTTCC TGACACTTTC AGAAAGTTGG AAAATCCACA GAGGGTTTCT
    GTACTGTTGA AGGATATTTC AGAAAATCTC GATTCATTGA GGAAGATGAT ATTTGGTTCT
    ACCGAGACCG AATTGAAGCC TAGCGGCAAT TCTAACTTAC TACCATTGGG AGACCCGACT
    GCTGGTGGAG TACTACAGAA ACAAATTAGA GAACATAAAA TTTTTACTGA AGTTAAAGAT
    GAGACATGGG ACACAACACT GGATAATTTC ATTAAACATG ATGGAGAAAA TGTACTTGGA
    AATGAAGTGA AAAGAAAAGA CGATGAATTT GAAGATGAAC TAGAAGCCAA TAAGTTGAAA
    GAGGAAACAG AAAGAATTAT GTCATTAGAT ATCACAGAAT ATGAACTCCA AATTTTGGAA
    CAGCAGGCTC AGGAAAAAAA TCTTAGTGAT GTTACTTACA AATCTCCTGA GCATGTATCC
    TCCAAGGATA ATGAAGAGGA TGTATCTTAT ATAATTGAAA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGACTTTAGA AAACCTAAAT ACTGTCACGG TAGATCCAAT TCATTCAAAA
    GGCACAGAAA TGGAAGGAAA TCTGGATGTT CCTTTTGAAG ATGAGGAAGA TGAGAGTGAA
    GCTGTTGAAG AGGAAGAAGA AGATGATGAA GACCGTTTGT TACCAGGACC TAATGCAGAG
    CAAGTGATTT GCCTCAAGAC CTACTTTGGC CATTCCAGTT TTAAGCCGGT TCAGTGGAAA
    GTGATTCATT CTGTATTGGA AGAAAGAAGA GATAACGTTG TTGTCATGGC AACTGGATAT
    GGAAAGAGTT TATGCTTCCA GTATCCACCA GTTTATGTAG GTGGGATTGC CATTGTCATC
    TCTCCTCTTA TTTCTTTGAT GGAAGACCAA GTGCTCCAGC TTGAAATGTC CAACATTCCA
    GCTTGTTTTC TGGGATCAGC ACAGTCAAAA AATGTTCTAG AGGATGTTAA ATTAGGCAAA
    TATCGGATTG TATACATAAC TCCAGAATTC TGTTCGAGGA ACTTGTACCT ACTCCACCAA
    CTTCAGGCTA ATATTGGTAT CACACTTATT GCTGTGGATG AGGCTCACTG TATTTCTGAG
    TGGGGACATG ATTTTAGAAA TTCATTCAGG AATTTGGGCT CCCTAAAAAC AGCATTCCCG
    TTGGTTCCAG TGGTCGCCCT CACTGCTACG GCCAGTTCTT CAATCCGGGA CGACATTGTG
    CGTTGCTTAG AACTGAAGAA CCCTCGGATC ACCTGTACTG GTTTTGATCG ACCGAACTTG
    TATTTAGAAG TTGGACAAAA AACAGGAAAC ATCCTTCAGG ATCTGAAGCA ATTTCTTGTC
    CAAAAGACAT GTTCTGCCTG GGAATTTGAA GGTCCAACTA TCATCTATTG TCTTTCCAGA
    AAAATGACAG AACAAGTTAC AGCTGAACTC AGGAAACTGC AGTTAGCCTG TGGAACATAC
    CATGCAGGCA TGGGTAGTAA CTCAAGGAAA GAGGTTCATC ATCGGTTCAT GAGAGATGAA
    ATTCAGTGTG TCGTAGCTAC CATAGCTTTT GGAATGGGCA TTAATAAAGC TGATATTCGC
    AAAGTCATTC ATTACGGTGC TCCTAAGGAA ATGGAATCAT ATTACCAGGA GATTGGTAGA
    GCTGGCCGGG ATGGACTTCA AAGTTCTTGT CACCTACTCT GGACTCCAAC AGACATCAAC
    TTAAATAGGA ACCGCCTTAG TGACATACCT GATGAAAAGT TTCGATTATA CAAATTAAAG
    ATGATGGAAA AGATGGAAAA ATATCTTCGT TCCAGCAAGT GTAGGCGACA AATCATCTTG
    TCTCATTTTG AAGATAAACA ACTACGAAAG GCCTCCGTGG GCATTATGGG AACTGAAAAT
    TGCTGTGATA ATTGCAGGTT CAGATTGGAT CATTGCTTTT TCATTGATGA CTCAGATGAT
    GCAGCCCAGG ACTTCGGTCC GCAAGCACTC CAGCTATTAT CTGCTGTGAA CATCTTAGGA
    GAAAAGTTTG GAATTGGGGT TCCAATTTTA TTTCTCCAAG GATCCAATTC CAAGCGTCTC
    GCAGATAAAT ATCGCAGTCA CAGTTTCTTT GGCACTGGCA AGGACCAAAC AAGGGCGTGG
    TGGAGGGCTC TTTCCCGTCA GCTCATAACT GAGGGATTCT TGGTCGAAGT GCCTGGGAAA
    GAAAAGTTTA TAAAGATTTG CACCCTTACA AAAAAGGGTA GAAATTGGCT TGATAAAGCC
    AAAAGAGGAT CCCCTCAGAG GCTAATCCTT GAAGCTAATG AAGAGCTGCG TCCAAGGAGG
    TTTCTTCTAC CGAGTTCTAA AACTGTATCT TCAGACACTG GACAGCATTC CTCTAGTCAA
    GTACCAACTG AATTAACTGC AGAGAATAAG CAGGCTAACT TGGAGAAGAT GTATTCTTAT
    AAAGCATTTG ATAAAATTTC TTCTGGGAGT AACATTCCTA AAAAAAGCGT CATGTTGGAA
    TCACCAAGAA AATCTTACAA GTCCTCAGAA CCTGTTATTT CAGCTCAAGA GCAAGAGTCT
    CAGACTCTGT TATACGGTGA ATTGGTAGAA GCTAGGCAGA AACGTGCCCA TAACATGGAC
    GTTCCTCCAG CTATCCTGGC AACAAATAAG ATACTGTTGG ATATGGCCAA AATGAGACCT
    ACTACGGTTG AAAATGTGAA AAGGATCGAC GGTGTTTCTG AAGGCAAAGC TACTATGTTG
    GCCCCTCTGT TGGAAGTCAT CAAACATTTC AGTCAAACAA ATAGTATTCA GACAGACCTC
    TTTCCAAGTA CAAAGCCTCA AGAACAGCAG AAAAGTCCGG TGGTGAAAGA TGAAGCACGC
    TCACTTTCAC ACTCTGTGGC CGTCACATAT TCTTTATTCC AAGAAAAGAA CATGTCTTTG
    AAAAGCATAT CTGAGAACAG GACTCTGCCT CTCACAGCAG TTGGCGCACA CTTACTCCAA
    GCGCTGAAAG CCGGCTACCC ACTCGACATG GAGCGAGCAG GCCTGACTCC AGAGACTCAC
    AAGATTATTG CTGATGTCAT CCGAAACCCT CCCGTCAACT CAGATATCAA TAAAACTAAA
    CTAATTAGAA TGTTTGTTCC TGAAAACATT GACACATACC TTATCTACAT GGCAATTGAG
    ATCCTGAGAA AAGATTGTGA CAACAGACCG TTATGCCAAC CTTCATGTGA TTCCAGTAAA
    AAGAGATGTT TCCCCAACTC TGAGGACAGC TGTTCACGTT CGAAGAGAAG CAAGGACGAA
    GTAGGCACTA ATACCAAGGT TGATGGAAAA CATCCTTGTG ATCTTTCACC AAAAGATAAG
    AGTCACAGAA ATTCACTGAG ACTGACATCT TGGAATCAGC CATCCAGCGA TCCAGAAATA
    GAAGATTTAT TTACAGACTC TCATCCACAG ACTTCATCTG GAGCCTTAAA GAGAATGTAT
    GCGGGGTTTG GCAAGAGAGA TAAGATCTTA GTTGATACCA GCAAAAAATC AATGGCCAGA
    ACGAAAAAGA AGGGTCTTTT TAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60387
    Clone ID Related Accession (Same CDS sequence) XM_023630600.1
    Accession Version XM_023630600.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGCTTG AAAACAAAGC AATTAAAAAA GTAGGTGTAG GAATTGAAGG GGATCAGTGG 
    AAACTTCTAC GTGACTTTGA CATCAAATTG AAGAGTTTTG TGGAGCTGAC AGATGTTGCA
    AATGAAAAGC TGAAATGTGC AGAGATCTGG AGCCTCAATG GTTTGGTTAA ACACCTCTTC
    GGTAAACAGC TTCTGAAAGA CAAGTCTGTC CGCTGTAGTG ATTGGAATAA TTTTCCTCTC
    ACTGAGGATC AGAAACTGTA TGCAGCCACG GATGCTTATG CTGGTTTCAT TATTTACCGA
    AAATTAGAAA TTTTGGGTGA TGCTGTGCAA ATGTTTACTA TAAATAGAGA GGAGGAACTT
    CTTTTTGGTG ACGTGAGGAA ACAGTTGACT TCAGTCTCCG AGGAGATGAT GGATCTGGCT
    AAGCGTCTTC CTGACACTTT CAGAAAGTTG GAAAATCCAC AGAGGGTTTC TGTACTGTTG
    AAGGATATTT CAGAAAATCT CGATTCATTG AGGAAGATGA TATTTGGTTC TACCGAGACC
    GAATTGAAGC CTAGCGGCAA TTCTAACTTA CTACCATTGG GAGACCCGAC TGCTGGTGGA
    GTACTACAGA AACAAATTAG AGAACATAAA ATTTTTACTG AAGTTAAAGA TGAGACATGG
    GACACAACAC TGGATAATTT CATTAAACAT GATGGAGAAA ATGTACTTGG AAATGAAGTG
    AAAAGAAAAG ACGATGAATT TGAAGATGAA CTAGAAGCCA ATAAGTTGAA AGAGGAAACA
    GAAAGAATTA TGTCATTAGA TATCACAGAA TATGAACTCC AAATTTTGGA ACAGCAGGCT
    CAGGAAAAAA ATCTTAGTGA TGTTACTTAC AAATCTCCTG AGCATGTATC CTCCAAGGAT
    AATGAAGAGG ATGTATCTTA TATAATTGAA AGTGATGAAG ATTTAGAAAT GGAGATGCTT
    AAGACTTTAG AAAACCTAAA TACTGTCACG GTAGATCCAA TTCATTCAAA AGGCACAGAA
    ATGGAAGGAA ATCTGGATGT TCCTTTTGAA GATGAGGAAG ATGAGAGTGA AGCTGTTGAA
    GAGGAAGAAG AAGATGATGA AGACCGTTTG TTACCAGGAC CTAATGCAGA GCAAGTGATT
    TGCCTCAAGA CCTACTTTGG CCATTCCAGT TTTAAGCCGG TTCAGTGGAA AGTGATTCAT
    TCTGTATTGG AAGAAAGAAG AGATAACGTT GTTGTCATGG CAACTGGATA TGGAAAGAGT
    TTATGCTTCC AGTATCCACC AGTTTATGTA GGTGGGATTG CCATTGTCAT CTCTCCTCTT
    ATTTCTTTGA TGGAAGACCA AGTGCTCCAG CTTGAAATGT CCAACATTCC AGCTTGTTTT
    CTGGGATCAG CACAGTCAAA AAATGTTCTA GAGGATGTTA AATTAGGCAA ATATCGGATT
    GTATACATAA CTCCAGAATT CTGTTCGAGG AACTTGTACC TACTCCACCA ACTTCAGGCT
    AATATTGGTA TCACACTTAT TGCTGTGGAT GAGGCTCACT GTATTTCTGA GTGGGGACAT
    GATTTTAGAA ATTCATTCAG GAATTTGGGC TCCCTAAAAA CAGCATTCCC GTTGGTTCCA
    GTGGTCGCCC TCACTGCTAC GGCCAGTTCT TCAATCCGGG ACGACATTGT GCGTTGCTTA
    GAACTGAAGA ACCCTCGGAT CACCTGTACT GGTTTTGATC GACCGAACTT GTATTTAGAA
    GTTGGACAAA AAACAGGAAA CATCCTTCAG GATCTGAAGC AATTTCTTGT CCAAAAGACA
    TGTTCTGCCT GGGAATTTGA AGGTCCAACT ATCATCTATT GTCTTTCCAG AAAAATGACA
    GAACAAGTTA CAGCTGAACT CAGGAAACTG CAGTTAGCCT GTGGAACATA CCATGCAGGC
    ATGGGTAGTA ACTCAAGGAA AGAGGTTCAT CATCGGTTCA TGAGAGATGA AATTCAGTGT
    GTCGTAGCTA CCATAGCTTT TGGAATGGGC ATTAATAAAG CTGATATTCG CAAAGTCATT
    CATTACGGTG CTCCTAAGGA AATGGAATCA TATTACCAGG AGATTGGTAG AGCTGGCCGG
    GATGGACTTC AAAGTTCTTG TCACCTACTC TGGACTCCAA CAGACATCAA CTTAAATAGG
    AACCGCCTTA GTGACATACC TGATGAAAAG TTTCGATTAT ACAAATTAAA GATGATGGAA
    AAGATGGAAA AATATCTTCG TTCCAGCAAG TGTAGGCGAC AAATCATCTT GTCTCATTTT
    GAAGATAAAC AACTACGAAA GGCCTCCGTG GGCATTATGG GAACTGAAAA TTGCTGTGAT
    AATTGCAGGT TCAGATTGGA TCATTGCTTT TTCATTGATG ACTCAGATGA TGCAGCCCAG
    GACTTCGGTC CGCAAGCACT CCAGCTATTA TCTGCTGTGA ACATCTTAGG AGAAAAGTTT
    GGAATTGGGG TTCCAATTTT ATTTCTCCAA GGATCCAATT CCAAGCGTCT CGCAGATAAA
    TATCGCAGTC ACAGTTTCTT TGGCACTGGC AAGGACCAAA CAAGGGCGTG GTGGAGGGCT
    CTTTCCCGTC AGCTCATAAC TGAGGGATTC TTGGTCGAAG TGCCTGGGAA AGAAAAGTTT
    ATAAAGATTT GCACCCTTAC AAAAAAGGGT AGAAATTGGC TTGATAAAGC CAAAAGAGGA
    TCCCCTCAGA GGCTAATCCT TGAAGCTAAT GAAGAGCTGC GTCCAAGGAG GTTTCTTCTA
    CCGAGTTCTA AAACTGTATC TTCAGACACT GGACAGCATT CCTCTAGTCA AGTACCAACT
    GAATTAACTG CAGAGAATAA GCAGGCTAAC TTGGAGAAGA TGTATTCTTA TAAAGCATTT
    GATAAAATTT CTTCTGGGAG TAACATTCCT AAAAAAAGCG TCATGTTGGA ATCACCAAGA
    AAATCTTACA AGTCCTCAGA ACCTGTTATT TCAGCTCAAG AGCAAGAGTC TCAGACTCTG
    TTATACGGTG AATTGGTAGA AGCTAGGCAG AAACGTGCCC ATAACATGGA CGTTCCTCCA
    GCTATCCTGG CAACAAATAA GATACTGTTG GATATGGCCA AAATGAGACC TACTACGGTT
    GAAAATGTGA AAAGGATCGA CGGTGTTTCT GAAGGCAAAG CTACTATGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CAGTCAAACA AATAGTATTC AGACAGACCT CTTTCCAAGT
    ACAAAGCCTC AAGAACAGCA GAAAAGTCCG GTGGTGAAAG ATGAAGCACG CTCACTTTCA
    CACTCTGTGG CCGTCACATA TTCTTTATTC CAAGAAAAGA ACATGTCTTT GAAAAGCATA
    TCTGAGAACA GGACTCTGCC TCTCACAGCA GTTGGCGCAC ACTTACTCCA AGCGCTGAAA
    GCCGGCTACC CACTCGACAT GGAGCGAGCA GGCCTGACTC CAGAGACTCA CAAGATTATT
    GCTGATGTCA TCCGAAACCC TCCCGTCAAC TCAGATATCA ATAAAACTAA ACTAATTAGA
    ATGTTTGTTC CTGAAAACAT TGACACATAC CTTATCTACA TGGCAATTGA GATCCTGAGA
    AAAGATTGTG ACAACAGACC GTTATGCCAA CCTTCATGTG ATTCCAGTAA AAAGAGATGT
    TTCCCCAACT CTGAGGACAG CTGTTCACGT TCGAAGAGAA GCAAGGACGA AGTAGGCACT
    AATACCAAGG TTGATGGAAA ACATCCTTGT GATCTTTCAC CAAAAGATAA GAGTCACAGA
    AATTCACTGA GACTGACATC TTGGAATCAG CCATCCAGCG ATCCAGAAAT AGAAGATTTA
    TTTACAGACT CTCATCCACA GACTTCATCT GGAGCCTTAA AGAGAATGTA TGCGGGGTTT
    GGCAAGAGAG ATAAGATCTT AGTTGATACC AGCAAAAAAT CAATGGCCAG AACGAAAAAG
    AAGGGTCTTT TTAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486368.1
    CDS140..4117
    Translation

    Target ORF information:

    RefSeq Version XM_023630600.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630600.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGTTGCTTG AAAACAAAGC AATTAAAAAA GTAGGTGTAG GAATTGAAGG GGATCAGTGG 
    AAACTTCTAC GTGACTTTGA CATCAAATTG AAGAGTTTTG TGGAGCTGAC AGATGTTGCA
    AATGAAAAGC TGAAATGTGC AGAGATCTGG AGCCTCAATG GTTTGGTTAA ACACCTCTTC
    GGTAAACAGC TTCTGAAAGA CAAGTCTGTC CGCTGTAGTG ATTGGAATAA TTTTCCTCTC
    ACTGAGGATC AGAAACTGTA TGCAGCCACG GATGCTTATG CTGGTTTCAT TATTTACCGA
    AAATTAGAAA TTTTGGGTGA TGCTGTGCAA ATGTTTACTA TAAATAGAGA GGAGGAACTT
    CTTTTTGGTG ACGTGAGGAA ACAGTTGACT TCAGTCTCCG AGGAGATGAT GGATCTGGCT
    AAGCGTCTTC CTGACACTTT CAGAAAGTTG GAAAATCCAC AGAGGGTTTC TGTACTGTTG
    AAGGATATTT CAGAAAATCT CGATTCATTG AGGAAGATGA TATTTGGTTC TACCGAGACC
    GAATTGAAGC CTAGCGGCAA TTCTAACTTA CTACCATTGG GAGACCCGAC TGCTGGTGGA
    GTACTACAGA AACAAATTAG AGAACATAAA ATTTTTACTG AAGTTAAAGA TGAGACATGG
    GACACAACAC TGGATAATTT CATTAAACAT GATGGAGAAA ATGTACTTGG AAATGAAGTG
    AAAAGAAAAG ACGATGAATT TGAAGATGAA CTAGAAGCCA ATAAGTTGAA AGAGGAAACA
    GAAAGAATTA TGTCATTAGA TATCACAGAA TATGAACTCC AAATTTTGGA ACAGCAGGCT
    CAGGAAAAAA ATCTTAGTGA TGTTACTTAC AAATCTCCTG AGCATGTATC CTCCAAGGAT
    AATGAAGAGG ATGTATCTTA TATAATTGAA AGTGATGAAG ATTTAGAAAT GGAGATGCTT
    AAGACTTTAG AAAACCTAAA TACTGTCACG GTAGATCCAA TTCATTCAAA AGGCACAGAA
    ATGGAAGGAA ATCTGGATGT TCCTTTTGAA GATGAGGAAG ATGAGAGTGA AGCTGTTGAA
    GAGGAAGAAG AAGATGATGA AGACCGTTTG TTACCAGGAC CTAATGCAGA GCAAGTGATT
    TGCCTCAAGA CCTACTTTGG CCATTCCAGT TTTAAGCCGG TTCAGTGGAA AGTGATTCAT
    TCTGTATTGG AAGAAAGAAG AGATAACGTT GTTGTCATGG CAACTGGATA TGGAAAGAGT
    TTATGCTTCC AGTATCCACC AGTTTATGTA GGTGGGATTG CCATTGTCAT CTCTCCTCTT
    ATTTCTTTGA TGGAAGACCA AGTGCTCCAG CTTGAAATGT CCAACATTCC AGCTTGTTTT
    CTGGGATCAG CACAGTCAAA AAATGTTCTA GAGGATGTTA AATTAGGCAA ATATCGGATT
    GTATACATAA CTCCAGAATT CTGTTCGAGG AACTTGTACC TACTCCACCA ACTTCAGGCT
    AATATTGGTA TCACACTTAT TGCTGTGGAT GAGGCTCACT GTATTTCTGA GTGGGGACAT
    GATTTTAGAA ATTCATTCAG GAATTTGGGC TCCCTAAAAA CAGCATTCCC GTTGGTTCCA
    GTGGTCGCCC TCACTGCTAC GGCCAGTTCT TCAATCCGGG ACGACATTGT GCGTTGCTTA
    GAACTGAAGA ACCCTCGGAT CACCTGTACT GGTTTTGATC GACCGAACTT GTATTTAGAA
    GTTGGACAAA AAACAGGAAA CATCCTTCAG GATCTGAAGC AATTTCTTGT CCAAAAGACA
    TGTTCTGCCT GGGAATTTGA AGGTCCAACT ATCATCTATT GTCTTTCCAG AAAAATGACA
    GAACAAGTTA CAGCTGAACT CAGGAAACTG CAGTTAGCCT GTGGAACATA CCATGCAGGC
    ATGGGTAGTA ACTCAAGGAA AGAGGTTCAT CATCGGTTCA TGAGAGATGA AATTCAGTGT
    GTCGTAGCTA CCATAGCTTT TGGAATGGGC ATTAATAAAG CTGATATTCG CAAAGTCATT
    CATTACGGTG CTCCTAAGGA AATGGAATCA TATTACCAGG AGATTGGTAG AGCTGGCCGG
    GATGGACTTC AAAGTTCTTG TCACCTACTC TGGACTCCAA CAGACATCAA CTTAAATAGG
    AACCGCCTTA GTGACATACC TGATGAAAAG TTTCGATTAT ACAAATTAAA GATGATGGAA
    AAGATGGAAA AATATCTTCG TTCCAGCAAG TGTAGGCGAC AAATCATCTT GTCTCATTTT
    GAAGATAAAC AACTACGAAA GGCCTCCGTG GGCATTATGG GAACTGAAAA TTGCTGTGAT
    AATTGCAGGT TCAGATTGGA TCATTGCTTT TTCATTGATG ACTCAGATGA TGCAGCCCAG
    GACTTCGGTC CGCAAGCACT CCAGCTATTA TCTGCTGTGA ACATCTTAGG AGAAAAGTTT
    GGAATTGGGG TTCCAATTTT ATTTCTCCAA GGATCCAATT CCAAGCGTCT CGCAGATAAA
    TATCGCAGTC ACAGTTTCTT TGGCACTGGC AAGGACCAAA CAAGGGCGTG GTGGAGGGCT
    CTTTCCCGTC AGCTCATAAC TGAGGGATTC TTGGTCGAAG TGCCTGGGAA AGAAAAGTTT
    ATAAAGATTT GCACCCTTAC AAAAAAGGGT AGAAATTGGC TTGATAAAGC CAAAAGAGGA
    TCCCCTCAGA GGCTAATCCT TGAAGCTAAT GAAGAGCTGC GTCCAAGGAG GTTTCTTCTA
    CCGAGTTCTA AAACTGTATC TTCAGACACT GGACAGCATT CCTCTAGTCA AGTACCAACT
    GAATTAACTG CAGAGAATAA GCAGGCTAAC TTGGAGAAGA TGTATTCTTA TAAAGCATTT
    GATAAAATTT CTTCTGGGAG TAACATTCCT AAAAAAAGCG TCATGTTGGA ATCACCAAGA
    AAATCTTACA AGTCCTCAGA ACCTGTTATT TCAGCTCAAG AGCAAGAGTC TCAGACTCTG
    TTATACGGTG AATTGGTAGA AGCTAGGCAG AAACGTGCCC ATAACATGGA CGTTCCTCCA
    GCTATCCTGG CAACAAATAA GATACTGTTG GATATGGCCA AAATGAGACC TACTACGGTT
    GAAAATGTGA AAAGGATCGA CGGTGTTTCT GAAGGCAAAG CTACTATGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CAGTCAAACA AATAGTATTC AGACAGACCT CTTTCCAAGT
    ACAAAGCCTC AAGAACAGCA GAAAAGTCCG GTGGTGAAAG ATGAAGCACG CTCACTTTCA
    CACTCTGTGG CCGTCACATA TTCTTTATTC CAAGAAAAGA ACATGTCTTT GAAAAGCATA
    TCTGAGAACA GGACTCTGCC TCTCACAGCA GTTGGCGCAC ACTTACTCCA AGCGCTGAAA
    GCCGGCTACC CACTCGACAT GGAGCGAGCA GGCCTGACTC CAGAGACTCA CAAGATTATT
    GCTGATGTCA TCCGAAACCC TCCCGTCAAC TCAGATATCA ATAAAACTAA ACTAATTAGA
    ATGTTTGTTC CTGAAAACAT TGACACATAC CTTATCTACA TGGCAATTGA GATCCTGAGA
    AAAGATTGTG ACAACAGACC GTTATGCCAA CCTTCATGTG ATTCCAGTAA AAAGAGATGT
    TTCCCCAACT CTGAGGACAG CTGTTCACGT TCGAAGAGAA GCAAGGACGA AGTAGGCACT
    AATACCAAGG TTGATGGAAA ACATCCTTGT GATCTTTCAC CAAAAGATAA GAGTCACAGA
    AATTCACTGA GACTGACATC TTGGAATCAG CCATCCAGCG ATCCAGAAAT AGAAGATTTA
    TTTACAGACT CTCATCCACA GACTTCATCT GGAGCCTTAA AGAGAATGTA TGCGGGGTTT
    GGCAAGAGAG ATAAGATCTT AGTTGATACC AGCAAAAAAT CAATGGCCAG AACGAAAAAG
    AAGGGTCTTT TTAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60388
    Clone ID Related Accession (Same CDS sequence) XM_023630601.1
    Accession Version XM_023630601.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG 
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486369.1
    CDS3326..6958
    Translation

    Target ORF information:

    RefSeq Version XM_023630601.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630601.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAAGGAGG AACTTCTTTT TGGTGACGTG AGGAAACAGT TGACTTCAGT CTCCGAGGAG 
    ATGATGGATC TGGCTAAGCG TCTTCCTGAC ACTTTCAGAA AGTTGGAAAA TCCACAGAGG
    GTTTCTGTAC TGTTGAAGGA TATTTCAGAA AATCTCGATT CATTGAGGAA GATGATATTT
    GGTTCTACCG AGACCGAATT GAAGCCTAGC GGCAATTCTA ACTTACTACC ATTGGGAGAC
    CCGACTGCTG GTGGAGTACT ACAGAAACAA ATTAGAGAAC ATAAAATTTT TACTGAAGTT
    AAAGATGAGA CATGGGACAC AACACTGGAT AATTTCATTA AACATGATGG AGAAAATGTA
    CTTGGAAATG AAGTGAAAAG AAAAGACGAT GAATTTGAAG ATGAACTAGA AGCCAATAAG
    TTGAAAGAGG AAACAGAAAG AATTATGTCA TTAGATATCA CAGAATATGA ACTCCAAATT
    TTGGAACAGC AGGCTCAGGA AAAAAATCTT AGTGATGTTA CTTACAAATC TCCTGAGCAT
    GTATCCTCCA AGGATAATGA AGAGGATGTA TCTTATATAA TTGAAAGTGA TGAAGATTTA
    GAAATGGAGA TGCTTAAGAC TTTAGAAAAC CTAAATACTG TCACGGTAGA TCCAATTCAT
    TCAAAAGGCA CAGAAATGGA AGGAAATCTG GATGTTCCTT TTGAAGATGA GGAAGATGAG
    AGTGAAGCTG TTGAAGAGGA AGAAGAAGAT GATGAAGACC GTTTGTTACC AGGACCTAAT
    GCAGAGCAAG TGATTTGCCT CAAGACCTAC TTTGGCCATT CCAGTTTTAA GCCGGTTCAG
    TGGAAAGTGA TTCATTCTGT ATTGGAAGAA AGAAGAGATA ACGTTGTTGT CATGGCAACT
    GGATATGGAA AGAGTTTATG CTTCCAGTAT CCACCAGTTT ATGTAGGTGG GATTGCCATT
    GTCATCTCTC CTCTTATTTC TTTGATGGAA GACCAAGTGC TCCAGCTTGA AATGTCCAAC
    ATTCCAGCTT GTTTTCTGGG ATCAGCACAG TCAAAAAATG TTCTAGAGGA TGTTAAATTA
    GGCAAATATC GGATTGTATA CATAACTCCA GAATTCTGTT CGAGGAACTT GTACCTACTC
    CACCAACTTC AGGCTAATAT TGGTATCACA CTTATTGCTG TGGATGAGGC TCACTGTATT
    TCTGAGTGGG GACATGATTT TAGAAATTCA TTCAGGAATT TGGGCTCCCT AAAAACAGCA
    TTCCCGTTGG TTCCAGTGGT CGCCCTCACT GCTACGGCCA GTTCTTCAAT CCGGGACGAC
    ATTGTGCGTT GCTTAGAACT GAAGAACCCT CGGATCACCT GTACTGGTTT TGATCGACCG
    AACTTGTATT TAGAAGTTGG ACAAAAAACA GGAAACATCC TTCAGGATCT GAAGCAATTT
    CTTGTCCAAA AGACATGTTC TGCCTGGGAA TTTGAAGGTC CAACTATCAT CTATTGTCTT
    TCCAGAAAAA TGACAGAACA AGTTACAGCT GAACTCAGGA AACTGCAGTT AGCCTGTGGA
    ACATACCATG CAGGCATGGG TAGTAACTCA AGGAAAGAGG TTCATCATCG GTTCATGAGA
    GATGAAATTC AGTGTGTCGT AGCTACCATA GCTTTTGGAA TGGGCATTAA TAAAGCTGAT
    ATTCGCAAAG TCATTCATTA CGGTGCTCCT AAGGAAATGG AATCATATTA CCAGGAGATT
    GGTAGAGCTG GCCGGGATGG ACTTCAAAGT TCTTGTCACC TACTCTGGAC TCCAACAGAC
    ATCAACTTAA ATAGGAACCG CCTTAGTGAC ATACCTGATG AAAAGTTTCG ATTATACAAA
    TTAAAGATGA TGGAAAAGAT GGAAAAATAT CTTCGTTCCA GCAAGTGTAG GCGACAAATC
    ATCTTGTCTC ATTTTGAAGA TAAACAACTA CGAAAGGCCT CCGTGGGCAT TATGGGAACT
    GAAAATTGCT GTGATAATTG CAGGTTCAGA TTGGATCATT GCTTTTTCAT TGATGACTCA
    GATGATGCAG CCCAGGACTT CGGTCCGCAA GCACTCCAGC TATTATCTGC TGTGAACATC
    TTAGGAGAAA AGTTTGGAAT TGGGGTTCCA ATTTTATTTC TCCAAGGATC CAATTCCAAG
    CGTCTCGCAG ATAAATATCG CAGTCACAGT TTCTTTGGCA CTGGCAAGGA CCAAACAAGG
    GCGTGGTGGA GGGCTCTTTC CCGTCAGCTC ATAACTGAGG GATTCTTGGT CGAAGTGCCT
    GGGAAAGAAA AGTTTATAAA GATTTGCACC CTTACAAAAA AGGGTAGAAA TTGGCTTGAT
    AAAGCCAAAA GAGGATCCCC TCAGAGGCTA ATCCTTGAAG CTAATGAAGA GCTGCGTCCA
    AGGAGGTTTC TTCTACCGAG TTCTAAAACT GTATCTTCAG ACACTGGACA GCATTCCTCT
    AGTCAAGTAC CAACTGAATT AACTGCAGAG AATAAGCAGG CTAACTTGGA GAAGATGTAT
    TCTTATAAAG CATTTGATAA AATTTCTTCT GGGAGTAACA TTCCTAAAAA AAGCGTCATG
    TTGGAATCAC CAAGAAAATC TTACAAGTCC TCAGAACCTG TTATTTCAGC TCAAGAGCAA
    GAGTCTCAGA CTCTGTTATA CGGTGAATTG GTAGAAGCTA GGCAGAAACG TGCCCATAAC
    ATGGACGTTC CTCCAGCTAT CCTGGCAACA AATAAGATAC TGTTGGATAT GGCCAAAATG
    AGACCTACTA CGGTTGAAAA TGTGAAAAGG ATCGACGGTG TTTCTGAAGG CAAAGCTACT
    ATGTTGGCCC CTCTGTTGGA AGTCATCAAA CATTTCAGTC AAACAAATAG TATTCAGACA
    GACCTCTTTC CAAGTACAAA GCCTCAAGAA CAGCAGAAAA GTCCGGTGGT GAAAGATGAA
    GCACGCTCAC TTTCACACTC TGTGGCCGTC ACATATTCTT TATTCCAAGA AAAGAACATG
    TCTTTGAAAA GCATATCTGA GAACAGGACT CTGCCTCTCA CAGCAGTTGG CGCACACTTA
    CTCCAAGCGC TGAAAGCCGG CTACCCACTC GACATGGAGC GAGCAGGCCT GACTCCAGAG
    ACTCACAAGA TTATTGCTGA TGTCATCCGA AACCCTCCCG TCAACTCAGA TATCAATAAA
    ACTAAACTAA TTAGAATGTT TGTTCCTGAA AACATTGACA CATACCTTAT CTACATGGCA
    ATTGAGATCC TGAGAAAAGA TTGTGACAAC AGACCGTTAT GCCAACCTTC ATGTGATTCC
    AGTAAAAAGA GATGTTTCCC CAACTCTGAG GACAGCTGTT CACGTTCGAA GAGAAGCAAG
    GACGAAGTAG GCACTAATAC CAAGGTTGAT GGAAAACATC CTTGTGATCT TTCACCAAAA
    GATAAGAGTC ACAGAAATTC ACTGAGACTG ACATCTTGGA ATCAGCCATC CAGCGATCCA
    GAAATAGAAG ATTTATTTAC AGACTCTCAT CCACAGACTT CATCTGGAGC CTTAAAGAGA
    ATGTATGCGG GGTTTGGCAA GAGAGATAAG ATCTTAGTTG ATACCAGCAA AAAATCAATG
    GCCAGAACGA AAAAGAAGGG TCTTTTTAGT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60389
    Clone ID Related Accession (Same CDS sequence) XM_023630602.1
    Accession Version XM_023630602.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGAGG AATGGTATGC ACATGACAAC AGCTGGAAGT TACAGCTGGC AGTCTGTGGA 
    GTGGACAAAC TGGTTTCTGT ACTGTTGAAG GATATTTCAG AAAATCTCGA TTCATTGAGG
    AAGATGATAT TTGGTTCTAC CGAGACCGAA TTGAAGCCTA GCGGCAATTC TAACTTACTA
    CCATTGGGAG ACCCGACTGC TGGTGGAGTA CTACAGAAAC AAATTAGAGA ACATAAAATT
    TTTACTGAAG TTAAAGATGA GACATGGGAC ACAACACTGG ATAATTTCAT TAAACATGAT
    GGAGAAAATG TACTTGGAAA TGAAGTGAAA AGAAAAGACG ATGAATTTGA AGATGAACTA
    GAAGCCAATA AGTTGAAAGA GGAAACAGAA AGAATTATGT CATTAGATAT CACAGAATAT
    GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAAAAAATC TTAGTGATGT TACTTACAAA
    TCTCCTGAGC ATGTATCCTC CAAGGATAAT GAAGAGGATG TATCTTATAT AATTGAAAGT
    GATGAAGATT TAGAAATGGA GATGCTTAAG ACTTTAGAAA ACCTAAATAC TGTCACGGTA
    GATCCAATTC ATTCAAAAGG CACAGAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT
    GAGGAAGATG AGAGTGAAGC TGTTGAAGAG GAAGAAGAAG ATGATGAAGA CCGTTTGTTA
    CCAGGACCTA ATGCAGAGCA AGTGATTTGC CTCAAGACCT ACTTTGGCCA TTCCAGTTTT
    AAGCCGGTTC AGTGGAAAGT GATTCATTCT GTATTGGAAG AAAGAAGAGA TAACGTTGTT
    GTCATGGCAA CTGGATATGG AAAGAGTTTA TGCTTCCAGT ATCCACCAGT TTATGTAGGT
    GGGATTGCCA TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT
    GAAATGTCCA ACATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAG
    GATGTTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCGAGGAAC
    TTGTACCTAC TCCACCAACT TCAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGAA TTTGGGCTCC
    CTAAAAACAG CATTCCCGTT GGTTCCAGTG GTCGCCCTCA CTGCTACGGC CAGTTCTTCA
    ATCCGGGACG ACATTGTGCG TTGCTTAGAA CTGAAGAACC CTCGGATCAC CTGTACTGGT
    TTTGATCGAC CGAACTTGTA TTTAGAAGTT GGACAAAAAA CAGGAAACAT CCTTCAGGAT
    CTGAAGCAAT TTCTTGTCCA AAAGACATGT TCTGCCTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC TTTCCAGAAA AATGACAGAA CAAGTTACAG CTGAACTCAG GAAACTGCAG
    TTAGCCTGTG GAACATACCA TGCAGGCATG GGTAGTAACT CAAGGAAAGA GGTTCATCAT
    CGGTTCATGA GAGATGAAAT TCAGTGTGTC GTAGCTACCA TAGCTTTTGG AATGGGCATT
    AATAAAGCTG ATATTCGCAA AGTCATTCAT TACGGTGCTC CTAAGGAAAT GGAATCATAT
    TACCAGGAGA TTGGTAGAGC TGGCCGGGAT GGACTTCAAA GTTCTTGTCA CCTACTCTGG
    ACTCCAACAG ACATCAACTT AAATAGGAAC CGCCTTAGTG ACATACCTGA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGAAAAG ATGGAAAAAT ATCTTCGTTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GATAAACAAC TACGAAAGGC CTCCGTGGGC
    ATTATGGGAA CTGAAAATTG CTGTGATAAT TGCAGGTTCA GATTGGATCA TTGCTTTTTC
    ATTGATGACT CAGATGATGC AGCCCAGGAC TTCGGTCCGC AAGCACTCCA GCTATTATCT
    GCTGTGAACA TCTTAGGAGA AAAGTTTGGA ATTGGGGTTC CAATTTTATT TCTCCAAGGA
    TCCAATTCCA AGCGTCTCGC AGATAAATAT CGCAGTCACA GTTTCTTTGG CACTGGCAAG
    GACCAAACAA GGGCGTGGTG GAGGGCTCTT TCCCGTCAGC TCATAACTGA GGGATTCTTG
    GTCGAAGTGC CTGGGAAAGA AAAGTTTATA AAGATTTGCA CCCTTACAAA AAAGGGTAGA
    AATTGGCTTG ATAAAGCCAA AAGAGGATCC CCTCAGAGGC TAATCCTTGA AGCTAATGAA
    GAGCTGCGTC CAAGGAGGTT TCTTCTACCG AGTTCTAAAA CTGTATCTTC AGACACTGGA
    CAGCATTCCT CTAGTCAAGT ACCAACTGAA TTAACTGCAG AGAATAAGCA GGCTAACTTG
    GAGAAGATGT ATTCTTATAA AGCATTTGAT AAAATTTCTT CTGGGAGTAA CATTCCTAAA
    AAAAGCGTCA TGTTGGAATC ACCAAGAAAA TCTTACAAGT CCTCAGAACC TGTTATTTCA
    GCTCAAGAGC AAGAGTCTCA GACTCTGTTA TACGGTGAAT TGGTAGAAGC TAGGCAGAAA
    CGTGCCCATA ACATGGACGT TCCTCCAGCT ATCCTGGCAA CAAATAAGAT ACTGTTGGAT
    ATGGCCAAAA TGAGACCTAC TACGGTTGAA AATGTGAAAA GGATCGACGG TGTTTCTGAA
    GGCAAAGCTA CTATGTTGGC CCCTCTGTTG GAAGTCATCA AACATTTCAG TCAAACAAAT
    AGTATTCAGA CAGACCTCTT TCCAAGTACA AAGCCTCAAG AACAGCAGAA AAGTCCGGTG
    GTGAAAGATG AAGCACGCTC ACTTTCACAC TCTGTGGCCG TCACATATTC TTTATTCCAA
    GAAAAGAACA TGTCTTTGAA AAGCATATCT GAGAACAGGA CTCTGCCTCT CACAGCAGTT
    GGCGCACACT TACTCCAAGC GCTGAAAGCC GGCTACCCAC TCGACATGGA GCGAGCAGGC
    CTGACTCCAG AGACTCACAA GATTATTGCT GATGTCATCC GAAACCCTCC CGTCAACTCA
    GATATCAATA AAACTAAACT AATTAGAATG TTTGTTCCTG AAAACATTGA CACATACCTT
    ATCTACATGG CAATTGAGAT CCTGAGAAAA GATTGTGACA ACAGACCGTT ATGCCAACCT
    TCATGTGATT CCAGTAAAAA GAGATGTTTC CCCAACTCTG AGGACAGCTG TTCACGTTCG
    AAGAGAAGCA AGGACGAAGT AGGCACTAAT ACCAAGGTTG ATGGAAAACA TCCTTGTGAT
    CTTTCACCAA AAGATAAGAG TCACAGAAAT TCACTGAGAC TGACATCTTG GAATCAGCCA
    TCCAGCGATC CAGAAATAGA AGATTTATTT ACAGACTCTC ATCCACAGAC TTCATCTGGA
    GCCTTAAAGA GAATGTATGC GGGGTTTGGC AAGAGAGATA AGATCTTAGT TGATACCAGC
    AAAAAATCAA TGGCCAGAAC GAAAAAGAAG GGTCTTTTTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486370.1
    CDS22..3606
    Translation

    Target ORF information:

    RefSeq Version XM_023630602.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGAGG AATGGTATGC ACATGACAAC AGCTGGAAGT TACAGCTGGC AGTCTGTGGA 
    GTGGACAAAC TGGTTTCTGT ACTGTTGAAG GATATTTCAG AAAATCTCGA TTCATTGAGG
    AAGATGATAT TTGGTTCTAC CGAGACCGAA TTGAAGCCTA GCGGCAATTC TAACTTACTA
    CCATTGGGAG ACCCGACTGC TGGTGGAGTA CTACAGAAAC AAATTAGAGA ACATAAAATT
    TTTACTGAAG TTAAAGATGA GACATGGGAC ACAACACTGG ATAATTTCAT TAAACATGAT
    GGAGAAAATG TACTTGGAAA TGAAGTGAAA AGAAAAGACG ATGAATTTGA AGATGAACTA
    GAAGCCAATA AGTTGAAAGA GGAAACAGAA AGAATTATGT CATTAGATAT CACAGAATAT
    GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAAAAAATC TTAGTGATGT TACTTACAAA
    TCTCCTGAGC ATGTATCCTC CAAGGATAAT GAAGAGGATG TATCTTATAT AATTGAAAGT
    GATGAAGATT TAGAAATGGA GATGCTTAAG ACTTTAGAAA ACCTAAATAC TGTCACGGTA
    GATCCAATTC ATTCAAAAGG CACAGAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT
    GAGGAAGATG AGAGTGAAGC TGTTGAAGAG GAAGAAGAAG ATGATGAAGA CCGTTTGTTA
    CCAGGACCTA ATGCAGAGCA AGTGATTTGC CTCAAGACCT ACTTTGGCCA TTCCAGTTTT
    AAGCCGGTTC AGTGGAAAGT GATTCATTCT GTATTGGAAG AAAGAAGAGA TAACGTTGTT
    GTCATGGCAA CTGGATATGG AAAGAGTTTA TGCTTCCAGT ATCCACCAGT TTATGTAGGT
    GGGATTGCCA TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT
    GAAATGTCCA ACATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAG
    GATGTTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCGAGGAAC
    TTGTACCTAC TCCACCAACT TCAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGAA TTTGGGCTCC
    CTAAAAACAG CATTCCCGTT GGTTCCAGTG GTCGCCCTCA CTGCTACGGC CAGTTCTTCA
    ATCCGGGACG ACATTGTGCG TTGCTTAGAA CTGAAGAACC CTCGGATCAC CTGTACTGGT
    TTTGATCGAC CGAACTTGTA TTTAGAAGTT GGACAAAAAA CAGGAAACAT CCTTCAGGAT
    CTGAAGCAAT TTCTTGTCCA AAAGACATGT TCTGCCTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC TTTCCAGAAA AATGACAGAA CAAGTTACAG CTGAACTCAG GAAACTGCAG
    TTAGCCTGTG GAACATACCA TGCAGGCATG GGTAGTAACT CAAGGAAAGA GGTTCATCAT
    CGGTTCATGA GAGATGAAAT TCAGTGTGTC GTAGCTACCA TAGCTTTTGG AATGGGCATT
    AATAAAGCTG ATATTCGCAA AGTCATTCAT TACGGTGCTC CTAAGGAAAT GGAATCATAT
    TACCAGGAGA TTGGTAGAGC TGGCCGGGAT GGACTTCAAA GTTCTTGTCA CCTACTCTGG
    ACTCCAACAG ACATCAACTT AAATAGGAAC CGCCTTAGTG ACATACCTGA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGAAAAG ATGGAAAAAT ATCTTCGTTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GATAAACAAC TACGAAAGGC CTCCGTGGGC
    ATTATGGGAA CTGAAAATTG CTGTGATAAT TGCAGGTTCA GATTGGATCA TTGCTTTTTC
    ATTGATGACT CAGATGATGC AGCCCAGGAC TTCGGTCCGC AAGCACTCCA GCTATTATCT
    GCTGTGAACA TCTTAGGAGA AAAGTTTGGA ATTGGGGTTC CAATTTTATT TCTCCAAGGA
    TCCAATTCCA AGCGTCTCGC AGATAAATAT CGCAGTCACA GTTTCTTTGG CACTGGCAAG
    GACCAAACAA GGGCGTGGTG GAGGGCTCTT TCCCGTCAGC TCATAACTGA GGGATTCTTG
    GTCGAAGTGC CTGGGAAAGA AAAGTTTATA AAGATTTGCA CCCTTACAAA AAAGGGTAGA
    AATTGGCTTG ATAAAGCCAA AAGAGGATCC CCTCAGAGGC TAATCCTTGA AGCTAATGAA
    GAGCTGCGTC CAAGGAGGTT TCTTCTACCG AGTTCTAAAA CTGTATCTTC AGACACTGGA
    CAGCATTCCT CTAGTCAAGT ACCAACTGAA TTAACTGCAG AGAATAAGCA GGCTAACTTG
    GAGAAGATGT ATTCTTATAA AGCATTTGAT AAAATTTCTT CTGGGAGTAA CATTCCTAAA
    AAAAGCGTCA TGTTGGAATC ACCAAGAAAA TCTTACAAGT CCTCAGAACC TGTTATTTCA
    GCTCAAGAGC AAGAGTCTCA GACTCTGTTA TACGGTGAAT TGGTAGAAGC TAGGCAGAAA
    CGTGCCCATA ACATGGACGT TCCTCCAGCT ATCCTGGCAA CAAATAAGAT ACTGTTGGAT
    ATGGCCAAAA TGAGACCTAC TACGGTTGAA AATGTGAAAA GGATCGACGG TGTTTCTGAA
    GGCAAAGCTA CTATGTTGGC CCCTCTGTTG GAAGTCATCA AACATTTCAG TCAAACAAAT
    AGTATTCAGA CAGACCTCTT TCCAAGTACA AAGCCTCAAG AACAGCAGAA AAGTCCGGTG
    GTGAAAGATG AAGCACGCTC ACTTTCACAC TCTGTGGCCG TCACATATTC TTTATTCCAA
    GAAAAGAACA TGTCTTTGAA AAGCATATCT GAGAACAGGA CTCTGCCTCT CACAGCAGTT
    GGCGCACACT TACTCCAAGC GCTGAAAGCC GGCTACCCAC TCGACATGGA GCGAGCAGGC
    CTGACTCCAG AGACTCACAA GATTATTGCT GATGTCATCC GAAACCCTCC CGTCAACTCA
    GATATCAATA AAACTAAACT AATTAGAATG TTTGTTCCTG AAAACATTGA CACATACCTT
    ATCTACATGG CAATTGAGAT CCTGAGAAAA GATTGTGACA ACAGACCGTT ATGCCAACCT
    TCATGTGATT CCAGTAAAAA GAGATGTTTC CCCAACTCTG AGGACAGCTG TTCACGTTCG
    AAGAGAAGCA AGGACGAAGT AGGCACTAAT ACCAAGGTTG ATGGAAAACA TCCTTGTGAT
    CTTTCACCAA AAGATAAGAG TCACAGAAAT TCACTGAGAC TGACATCTTG GAATCAGCCA
    TCCAGCGATC CAGAAATAGA AGATTTATTT ACAGACTCTC ATCCACAGAC TTCATCTGGA
    GCCTTAAAGA GAATGTATGC GGGGTTTGGC AAGAGAGATA AGATCTTAGT TGATACCAGC
    AAAAAATCAA TGGCCAGAAC GAAAAAGAAG GGTCTTTTTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb60390
    Clone ID Related Accession (Same CDS sequence) XM_023630603.1
    Accession Version XM_023630603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product Werner syndrome ATP-dependent helicase isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009170.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAACCA GGGTTTCTGT ACTGTTGAAG GATATTTCAG AAAATCTCGA TTCATTGAGG 
    AAGATGATAT TTGGTTCTAC CGAGACCGAA TTGAAGCCTA GCGGCAATTC TAACTTACTA
    CCATTGGGAG ACCCGACTGC TGGTGGAGTA CTACAGAAAC AAATTAGAGA ACATAAAATT
    TTTACTGAAG TTAAAGATGA GACATGGGAC ACAACACTGG ATAATTTCAT TAAACATGAT
    GGAGAAAATG TACTTGGAAA TGAAGTGAAA AGAAAAGACG ATGAATTTGA AGATGAACTA
    GAAGCCAATA AGTTGAAAGA GGAAACAGAA AGAATTATGT CATTAGATAT CACAGAATAT
    GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAAAAAATC TTAGTGATGT TACTTACAAA
    TCTCCTGAGC ATGTATCCTC CAAGGATAAT GAAGAGGATG TATCTTATAT AATTGAAAGT
    GATGAAGATT TAGAAATGGA GATGCTTAAG ACTTTAGAAA ACCTAAATAC TGTCACGGTA
    GATCCAATTC ATTCAAAAGG CACAGAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT
    GAGGAAGATG AGAGTGAAGC TGTTGAAGAG GAAGAAGAAG ATGATGAAGA CCGTTTGTTA
    CCAGGACCTA ATGCAGAGCA AGTGATTTGC CTCAAGACCT ACTTTGGCCA TTCCAGTTTT
    AAGCCGGTTC AGTGGAAAGT GATTCATTCT GTATTGGAAG AAAGAAGAGA TAACGTTGTT
    GTCATGGCAA CTGGATATGG AAAGAGTTTA TGCTTCCAGT ATCCACCAGT TTATGTAGGT
    GGGATTGCCA TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT
    GAAATGTCCA ACATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAG
    GATGTTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCGAGGAAC
    TTGTACCTAC TCCACCAACT TCAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGAA TTTGGGCTCC
    CTAAAAACAG CATTCCCGTT GGTTCCAGTG GTCGCCCTCA CTGCTACGGC CAGTTCTTCA
    ATCCGGGACG ACATTGTGCG TTGCTTAGAA CTGAAGAACC CTCGGATCAC CTGTACTGGT
    TTTGATCGAC CGAACTTGTA TTTAGAAGTT GGACAAAAAA CAGGAAACAT CCTTCAGGAT
    CTGAAGCAAT TTCTTGTCCA AAAGACATGT TCTGCCTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC TTTCCAGAAA AATGACAGAA CAAGTTACAG CTGAACTCAG GAAACTGCAG
    TTAGCCTGTG GAACATACCA TGCAGGCATG GGTAGTAACT CAAGGAAAGA GGTTCATCAT
    CGGTTCATGA GAGATGAAAT TCAGTGTGTC GTAGCTACCA TAGCTTTTGG AATGGGCATT
    AATAAAGCTG ATATTCGCAA AGTCATTCAT TACGGTGCTC CTAAGGAAAT GGAATCATAT
    TACCAGGAGA TTGGTAGAGC TGGCCGGGAT GGACTTCAAA GTTCTTGTCA CCTACTCTGG
    ACTCCAACAG ACATCAACTT AAATAGGAAC CGCCTTAGTG ACATACCTGA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGAAAAG ATGGAAAAAT ATCTTCGTTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GATAAACAAC TACGAAAGGC CTCCGTGGGC
    ATTATGGGAA CTGAAAATTG CTGTGATAAT TGCAGGTTCA GATTGGATCA TTGCTTTTTC
    ATTGATGACT CAGATGATGC AGCCCAGGAC TTCGGTCCGC AAGCACTCCA GCTATTATCT
    GCTGTGAACA TCTTAGGAGA AAAGTTTGGA ATTGGGGTTC CAATTTTATT TCTCCAAGGA
    TCCAATTCCA AGCGTCTCGC AGATAAATAT CGCAGTCACA GTTTCTTTGG CACTGGCAAG
    GACCAAACAA GGGCGTGGTG GAGGGCTCTT TCCCGTCAGC TCATAACTGA GGGATTCTTG
    GTCGAAGTGC CTGGGAAAGA AAAGTTTATA AAGATTTGCA CCCTTACAAA AAAGGGTAGA
    AATTGGCTTG ATAAAGCCAA AAGAGGATCC CCTCAGAGGC TAATCCTTGA AGCTAATGAA
    GAGCTGCGTC CAAGGAGGTT TCTTCTACCG AGTTCTAAAA CTGTATCTTC AGACACTGGA
    CAGCATTCCT CTAGTCAAGT ACCAACTGAA TTAACTGCAG AGAATAAGCA GGCTAACTTG
    GAGAAGATGT ATTCTTATAA AGCATTTGAT AAAATTTCTT CTGGGAGTAA CATTCCTAAA
    AAAAGCGTCA TGTTGGAATC ACCAAGAAAA TCTTACAAGT CCTCAGAACC TGTTATTTCA
    GCTCAAGAGC AAGAGTCTCA GACTCTGTTA TACGGTGAAT TGGTAGAAGC TAGGCAGAAA
    CGTGCCCATA ACATGGACGT TCCTCCAGCT ATCCTGGCAA CAAATAAGAT ACTGTTGGAT
    ATGGCCAAAA TGAGACCTAC TACGGTTGAA AATGTGAAAA GGATCGACGG TGTTTCTGAA
    GGCAAAGCTA CTATGTTGGC CCCTCTGTTG GAAGTCATCA AACATTTCAG TCAAACAAAT
    AGTATTCAGA CAGACCTCTT TCCAAGTACA AAGCCTCAAG AACAGCAGAA AAGTCCGGTG
    GTGAAAGATG AAGCACGCTC ACTTTCACAC TCTGTGGCCG TCACATATTC TTTATTCCAA
    GAAAAGAACA TGTCTTTGAA AAGCATATCT GAGAACAGGA CTCTGCCTCT CACAGCAGTT
    GGCGCACACT TACTCCAAGC GCTGAAAGCC GGCTACCCAC TCGACATGGA GCGAGCAGGC
    CTGACTCCAG AGACTCACAA GATTATTGCT GATGTCATCC GAAACCCTCC CGTCAACTCA
    GATATCAATA AAACTAAACT AATTAGAATG TTTGTTCCTG AAAACATTGA CACATACCTT
    ATCTACATGG CAATTGAGAT CCTGAGAAAA GATTGTGACA ACAGACCGTT ATGCCAACCT
    TCATGTGATT CCAGTAAAAA GAGATGTTTC CCCAACTCTG AGGACAGCTG TTCACGTTCG
    AAGAGAAGCA AGGACGAAGT AGGCACTAAT ACCAAGGTTG ATGGAAAACA TCCTTGTGAT
    CTTTCACCAA AAGATAAGAG TCACAGAAAT TCACTGAGAC TGACATCTTG GAATCAGCCA
    TCCAGCGATC CAGAAATAGA AGATTTATTT ACAGACTCTC ATCCACAGAC TTCATCTGGA
    GCCTTAAAGA GAATGTATGC GGGGTTTGGC AAGAGAGATA AGATCTTAGT TGATACCAGC
    AAAAAATCAA TGGCCAGAAC GAAAAAGAAG GGTCTTTTTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023486371.1
    CDS22..3546
    Translation

    Target ORF information:

    RefSeq Version XM_023630603.1
    Organism Equus caballus(horse)
    Definition Equus caballus Werner syndrome RecQ like helicase (WRN), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023630603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAAACCA GGGTTTCTGT ACTGTTGAAG GATATTTCAG AAAATCTCGA TTCATTGAGG 
    AAGATGATAT TTGGTTCTAC CGAGACCGAA TTGAAGCCTA GCGGCAATTC TAACTTACTA
    CCATTGGGAG ACCCGACTGC TGGTGGAGTA CTACAGAAAC AAATTAGAGA ACATAAAATT
    TTTACTGAAG TTAAAGATGA GACATGGGAC ACAACACTGG ATAATTTCAT TAAACATGAT
    GGAGAAAATG TACTTGGAAA TGAAGTGAAA AGAAAAGACG ATGAATTTGA AGATGAACTA
    GAAGCCAATA AGTTGAAAGA GGAAACAGAA AGAATTATGT CATTAGATAT CACAGAATAT
    GAACTCCAAA TTTTGGAACA GCAGGCTCAG GAAAAAAATC TTAGTGATGT TACTTACAAA
    TCTCCTGAGC ATGTATCCTC CAAGGATAAT GAAGAGGATG TATCTTATAT AATTGAAAGT
    GATGAAGATT TAGAAATGGA GATGCTTAAG ACTTTAGAAA ACCTAAATAC TGTCACGGTA
    GATCCAATTC ATTCAAAAGG CACAGAAATG GAAGGAAATC TGGATGTTCC TTTTGAAGAT
    GAGGAAGATG AGAGTGAAGC TGTTGAAGAG GAAGAAGAAG ATGATGAAGA CCGTTTGTTA
    CCAGGACCTA ATGCAGAGCA AGTGATTTGC CTCAAGACCT ACTTTGGCCA TTCCAGTTTT
    AAGCCGGTTC AGTGGAAAGT GATTCATTCT GTATTGGAAG AAAGAAGAGA TAACGTTGTT
    GTCATGGCAA CTGGATATGG AAAGAGTTTA TGCTTCCAGT ATCCACCAGT TTATGTAGGT
    GGGATTGCCA TTGTCATCTC TCCTCTTATT TCTTTGATGG AAGACCAAGT GCTCCAGCTT
    GAAATGTCCA ACATTCCAGC TTGTTTTCTG GGATCAGCAC AGTCAAAAAA TGTTCTAGAG
    GATGTTAAAT TAGGCAAATA TCGGATTGTA TACATAACTC CAGAATTCTG TTCGAGGAAC
    TTGTACCTAC TCCACCAACT TCAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGAA TTTGGGCTCC
    CTAAAAACAG CATTCCCGTT GGTTCCAGTG GTCGCCCTCA CTGCTACGGC CAGTTCTTCA
    ATCCGGGACG ACATTGTGCG TTGCTTAGAA CTGAAGAACC CTCGGATCAC CTGTACTGGT
    TTTGATCGAC CGAACTTGTA TTTAGAAGTT GGACAAAAAA CAGGAAACAT CCTTCAGGAT
    CTGAAGCAAT TTCTTGTCCA AAAGACATGT TCTGCCTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC TTTCCAGAAA AATGACAGAA CAAGTTACAG CTGAACTCAG GAAACTGCAG
    TTAGCCTGTG GAACATACCA TGCAGGCATG GGTAGTAACT CAAGGAAAGA GGTTCATCAT
    CGGTTCATGA GAGATGAAAT TCAGTGTGTC GTAGCTACCA TAGCTTTTGG AATGGGCATT
    AATAAAGCTG ATATTCGCAA AGTCATTCAT TACGGTGCTC CTAAGGAAAT GGAATCATAT
    TACCAGGAGA TTGGTAGAGC TGGCCGGGAT GGACTTCAAA GTTCTTGTCA CCTACTCTGG
    ACTCCAACAG ACATCAACTT AAATAGGAAC CGCCTTAGTG ACATACCTGA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGAAAAG ATGGAAAAAT ATCTTCGTTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GATAAACAAC TACGAAAGGC CTCCGTGGGC
    ATTATGGGAA CTGAAAATTG CTGTGATAAT TGCAGGTTCA GATTGGATCA TTGCTTTTTC
    ATTGATGACT CAGATGATGC AGCCCAGGAC TTCGGTCCGC AAGCACTCCA GCTATTATCT
    GCTGTGAACA TCTTAGGAGA AAAGTTTGGA ATTGGGGTTC CAATTTTATT TCTCCAAGGA
    TCCAATTCCA AGCGTCTCGC AGATAAATAT CGCAGTCACA GTTTCTTTGG CACTGGCAAG
    GACCAAACAA GGGCGTGGTG GAGGGCTCTT TCCCGTCAGC TCATAACTGA GGGATTCTTG
    GTCGAAGTGC CTGGGAAAGA AAAGTTTATA AAGATTTGCA CCCTTACAAA AAAGGGTAGA
    AATTGGCTTG ATAAAGCCAA AAGAGGATCC CCTCAGAGGC TAATCCTTGA AGCTAATGAA
    GAGCTGCGTC CAAGGAGGTT TCTTCTACCG AGTTCTAAAA CTGTATCTTC AGACACTGGA
    CAGCATTCCT CTAGTCAAGT ACCAACTGAA TTAACTGCAG AGAATAAGCA GGCTAACTTG
    GAGAAGATGT ATTCTTATAA AGCATTTGAT AAAATTTCTT CTGGGAGTAA CATTCCTAAA
    AAAAGCGTCA TGTTGGAATC ACCAAGAAAA TCTTACAAGT CCTCAGAACC TGTTATTTCA
    GCTCAAGAGC AAGAGTCTCA GACTCTGTTA TACGGTGAAT TGGTAGAAGC TAGGCAGAAA
    CGTGCCCATA ACATGGACGT TCCTCCAGCT ATCCTGGCAA CAAATAAGAT ACTGTTGGAT
    ATGGCCAAAA TGAGACCTAC TACGGTTGAA AATGTGAAAA GGATCGACGG TGTTTCTGAA
    GGCAAAGCTA CTATGTTGGC CCCTCTGTTG GAAGTCATCA AACATTTCAG TCAAACAAAT
    AGTATTCAGA CAGACCTCTT TCCAAGTACA AAGCCTCAAG AACAGCAGAA AAGTCCGGTG
    GTGAAAGATG AAGCACGCTC ACTTTCACAC TCTGTGGCCG TCACATATTC TTTATTCCAA
    GAAAAGAACA TGTCTTTGAA AAGCATATCT GAGAACAGGA CTCTGCCTCT CACAGCAGTT
    GGCGCACACT TACTCCAAGC GCTGAAAGCC GGCTACCCAC TCGACATGGA GCGAGCAGGC
    CTGACTCCAG AGACTCACAA GATTATTGCT GATGTCATCC GAAACCCTCC CGTCAACTCA
    GATATCAATA AAACTAAACT AATTAGAATG TTTGTTCCTG AAAACATTGA CACATACCTT
    ATCTACATGG CAATTGAGAT CCTGAGAAAA GATTGTGACA ACAGACCGTT ATGCCAACCT
    TCATGTGATT CCAGTAAAAA GAGATGTTTC CCCAACTCTG AGGACAGCTG TTCACGTTCG
    AAGAGAAGCA AGGACGAAGT AGGCACTAAT ACCAAGGTTG ATGGAAAACA TCCTTGTGAT
    CTTTCACCAA AAGATAAGAG TCACAGAAAT TCACTGAGAC TGACATCTTG GAATCAGCCA
    TCCAGCGATC CAGAAATAGA AGATTTATTT ACAGACTCTC ATCCACAGAC TTCATCTGGA
    GCCTTAAAGA GAATGTATGC GGGGTTTGGC AAGAGAGATA AGATCTTAGT TGATACCAGC
    AAAAAATCAA TGGCCAGAAC GAAAAAGAAG GGTCTTTTTA GTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.