MTHFR cDNA ORF clone, Equus caballus(horse)

The following MTHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb17129 XM_023635784.1
Latest version!
Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHb17129 XM_005607524.3
Latest version!
Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHb17129 XM_005607523.3
Latest version!
Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHb17129 XM_023635783.1
Latest version!
Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHb17128 XM_023635781.1
Latest version!
Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OHb29377 XM_014737646.1
Latest version!
Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OHb29378 XM_014737647.1
Latest version!
Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OHb17129 XM_005607523.2 Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHb17129 XM_005607524.2 Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb17129
    Clone ID Related Accession (Same CDS sequence) XM_005607524.3 , XM_023635783.1 , XM_023635784.1 , XM_005607523.2 , XM_005607523.3 , XM_005607524.2
    Accession Version XM_005607524.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009145.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005607524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005607581.1
    CDS239..2206
    Translation

    Target ORF information:

    RefSeq Version XM_005607524.3
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005607524.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb17129
    Clone ID Related Accession (Same CDS sequence) XM_005607524.3 , XM_023635783.1 , XM_023635784.1 , XM_005607523.2 , XM_005607523.3 , XM_005607524.2
    Accession Version XM_023635783.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009145.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023491551.1
    CDS451..2418
    Translation

    Target ORF information:

    RefSeq Version XM_023635783.1
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023635783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb17129
    Clone ID Related Accession (Same CDS sequence) XM_005607524.3 , XM_023635783.1 , XM_023635784.1 , XM_005607523.2 , XM_005607523.3 , XM_005607524.2
    Accession Version XM_023635784.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009145.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023491552.1
    CDS121..2088
    Translation

    Target ORF information:

    RefSeq Version XM_023635784.1
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023635784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb17129
    Clone ID Related Accession (Same CDS sequence) XM_005607524.3 , XM_023635783.1 , XM_023635784.1 , XM_005607523.2 , XM_005607523.3 , XM_005607524.2
    Accession Version XM_005607523.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867403.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005607523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005607580.1
    CDS267..2234
    Translation

    Target ORF information:

    RefSeq Version XM_005607523.2
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005607523.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb17129
    Clone ID Related Accession (Same CDS sequence) XM_005607524.3 , XM_023635783.1 , XM_023635784.1 , XM_005607523.2 , XM_005607523.3 , XM_005607524.2
    Accession Version XM_005607523.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009145.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_005607523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005607580.1
    CDS285..2252
    Translation

    Target ORF information:

    RefSeq Version XM_005607523.3
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005607523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb17129
    Clone ID Related Accession (Same CDS sequence) XM_005607524.3 , XM_023635783.1 , XM_023635784.1 , XM_005607523.2 , XM_005607523.3 , XM_005607524.2
    Accession Version XM_005607524.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867403.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005607524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005607581.1
    CDS221..2188
    Translation

    Target ORF information:

    RefSeq Version XM_005607524.2
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005607524.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAATG AACCCAGAGG GAATGGTGGC CCCATCCCCC GCTCGGATGG CAGCAGCAGT 
    GGCAGCGAGA GCTCCAGAGA TAGTTCAAGA TGTTCCACCC CGGGGCTGGA CCCCGAGCGG
    CACGAGAGAC TCAGGGAGAA GATGAGGCGG AGGATGGAGT CTGGTGACAA GTGGTTCTCC
    CTAGAATTCT TCCCTCCTCG GACTGCCCAG GGAGCTGTCA ATCTCATCTC AAGGTTTGAC
    CAGATGGGGG CTGGTGGCCC CCTCTTCATA GATGTGACCT GGCACCCAGC AGGGGACCCC
    GGCTCGGACA AGGAGACCTC CTCTATGATG ATTGCCAGCA CTGCTGTGAA CTACTGTGGC
    CTGGAGACCG TCCTGCACAT GACCTGCTGC CGCCAGAGCC GGGAAGAGAT CACCGGCTAT
    CTGCACAAGG CCAAGCGGCT GGGCCTGAAG AACATCTTGG CGTTGAGGGG AGACCCTGTA
    GGTGACCAGT GGGAAGAGGA GGAAGGTGGC TTCAGCTATG CTGCAGACTT GGTGAAGCAT
    ATCCGGAGCG AGTTCGGTGA CTACTTTGAC GTCTGTGTGG CAGGTTACCC CAAAGGCCAC
    CCTGACTCGG GGAGCTTTGA GGCCGACCTG AAGTACTTGA AGGAGAAGGT GTCTGCAGGA
    GCCGACTTCA TCATCACTCA GCTTTTCTTT GAGGCTGACA CGTTCTTCCA CTTTGTTAAG
    GCTTGCTCCG AGATAGGCAT TACCTGCCCC ATCCTCCCCG GCATCTTTCC TATTCAGGGC
    TACCACTCCC TTCGGCAGCT GGTGAAGCTG TCGAAGCTGG AAGTGCCGCA GGAGATCAAA
    GACGTGATCG AGCCAATCAA AGACAACGAC GGCGCCATCC GCAACTATGG CATCGACCAG
    GCCGTGAGCC TGTGCCAGCA GCTGCTGGCA AGTGGCTTGG TGCCAGGCCT CCACTTCTAT
    ACCCTCAACC GTGAGGTGGC CACCACAGAG GTGCTGAAGC GCCTGGGCAT GTGGGTCGAG
    GACCCCAGGC GCCCCCTGCC CTGGGCTCTC AGTGCCCACC CCAAGCGCCG GGAGGAAGAT
    GTCCGTCCCA TCTTCTGGGC CACCAGACCC AAGAGTTACA TCTACCGCAC CCAGGAGTGG
    GATGAGTTCC CCAACGGCCG CTGGGGCAAT TCCTCCTCGC CCGCCTTCGG GGAGCTGAAG
    GACTACTACC TCTTCTACCT GAAGAGCAAG TCCCCGAAGG AGGAGCTGCT GAAGATGTGG
    GGGGAGGAGC TGACCAGCGA GGAGAGCGTC TTCGAAGTCT TTGCCCACTA CCTCTCGGGA
    GAGCCCAACC AGAACGGCTA TAAAGTGACT TGCCTGCCCT GGAATGATGA GCCCTTGGCT
    GCCGAGACGA GCCTGATGAA GGAGGAGCTG CTGCGAGTGA ACCGGCAGGG CGTCCTCACC
    ATCAACTCCC AGCCCAACAT CAATGGGAAG CCGTCCTCCG ACCCCGTCGT GGGCTGGGGC
    CCCAGCGGGG GCTATGTCTT CCAGAAGGCC TACTTAGAGT TCTTCACTTC CCGCGAGACA
    GTGGAAGCGC TGCTGCAGGT GCTGAAGAAA TATGAGCTCC GGGTCAACTA CCACATCGTG
    GACGTGAAGG GTGAAAACAT CACCAATGCT CCTGAGCTGC AGCCCAACGC CGTCACTTGG
    GGCATCTTCC CTGGGCGAGA GATCATCCAG CCCACGGTGG TGGATCCCAT CAGCTTCATG
    TTCTGGAAGG ATGAGGCCTT CGCCCTGTGG ATTGAGCAGT GGGGCAAGCT GTACGAGGAG
    GAGTCCCCGT CCCGCATGAT CATCCAGTAC ATCCACGACA ACTACTTCCT GGTCAACCTG
    GTGGACAATG ACTTTCCGCT GGACAACTGC CTGTGGCAGG TGGTAGAAGA CACATTTGAG
    CTTCTCAACA GGCCTGCCCC GAATGAGAGG GAGATGGAGG CTCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb17128
    Clone ID Related Accession (Same CDS sequence) XM_023635781.1
    Accession Version XM_023635781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009145.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC CAAAACCCAA GGTCCTCCTA GCCAGGCACT GCTGCCCCTC CCTAGGAATG 
    TGGGCCTCAG AGGCCGGCAG TGTGAGGTTC TCTGTGCCGC CCTCCATCAG CAGGAACCGG
    GCCATGGTGA ATGAACCCAG AGGGAATGGT GGCCCCATCC CCCGCTCGGA TGGCAGCAGC
    AGTGGCAGCG AGAGCTCCAG AGATAGTTCA AGATGTTCCA CCCCGGGGCT GGACCCCGAG
    CGGCACGAGA GACTCAGGGA GAAGATGAGG CGGAGGATGG AGTCTGGTGA CAAGTGGTTC
    TCCCTAGAAT TCTTCCCTCC TCGGACTGCC CAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCAGATGG GGGCTGGTGG CCCCCTCTTC ATAGATGTGA CCTGGCACCC AGCAGGGGAC
    CCCGGCTCGG ACAAGGAGAC CTCCTCTATG ATGATTGCCA GCACTGCTGT GAACTACTGT
    GGCCTGGAGA CCGTCCTGCA CATGACCTGC TGCCGCCAGA GCCGGGAAGA GATCACCGGC
    TATCTGCACA AGGCCAAGCG GCTGGGCCTG AAGAACATCT TGGCGTTGAG GGGAGACCCT
    GTAGGTGACC AGTGGGAAGA GGAGGAAGGT GGCTTCAGCT ATGCTGCAGA CTTGGTGAAG
    CATATCCGGA GCGAGTTCGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCTGACT CGGGGAGCTT TGAGGCCGAC CTGAAGTACT TGAAGGAGAA GGTGTCTGCA
    GGAGCCGACT TCATCATCAC TCAGCTTTTC TTTGAGGCTG ACACGTTCTT CCACTTTGTT
    AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT TCCTATTCAG
    GGCTACCACT CCCTTCGGCA GCTGGTGAAG CTGTCGAAGC TGGAAGTGCC GCAGGAGATC
    AAAGACGTGA TCGAGCCAAT CAAAGACAAC GACGGCGCCA TCCGCAACTA TGGCATCGAC
    CAGGCCGTGA GCCTGTGCCA GCAGCTGCTG GCAAGTGGCT TGGTGCCAGG CCTCCACTTC
    TATACCCTCA ACCGTGAGGT GGCCACCACA GAGGTGCTGA AGCGCCTGGG CATGTGGGTC
    GAGGACCCCA GGCGCCCCCT GCCCTGGGCT CTCAGTGCCC ACCCCAAGCG CCGGGAGGAA
    GATGTCCGTC CCATCTTCTG GGCCACCAGA CCCAAGAGTT ACATCTACCG CACCCAGGAG
    TGGGATGAGT TCCCCAACGG CCGCTGGGGC AATTCCTCCT CGCCCGCCTT CGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCGA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG CGAGGAGAGC GTCTTCGAAG TCTTTGCCCA CTACCTCTCG
    GGAGAGCCCA ACCAGAACGG CTATAAAGTG ACTTGCCTGC CCTGGAATGA TGAGCCCTTG
    GCTGCCGAGA CGAGCCTGAT GAAGGAGGAG CTGCTGCGAG TGAACCGGCA GGGCGTCCTC
    ACCATCAACT CCCAGCCCAA CATCAATGGG AAGCCGTCCT CCGACCCCGT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTCTTCAC TTCCCGCGAG
    ACAGTGGAAG CGCTGCTGCA GGTGCTGAAG AAATATGAGC TCCGGGTCAA CTACCACATC
    GTGGACGTGA AGGGTGAAAA CATCACCAAT GCTCCTGAGC TGCAGCCCAA CGCCGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACGG TGGTGGATCC CATCAGCTTC
    ATGTTCTGGA AGGATGAGGC CTTCGCCCTG TGGATTGAGC AGTGGGGCAA GCTGTACGAG
    GAGGAGTCCC CGTCCCGCAT GATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTTCC GCTGGACAAC TGCCTGTGGC AGGTGGTAGA AGACACATTT
    GAGCTTCTCA ACAGGCCTGC CCCGAATGAG AGGGAGATGG AGGCTCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023491549.1
    CDS1463..3553
    Translation

    Target ORF information:

    RefSeq Version XM_023635781.1
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023635781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC CAAAACCCAA GGTCCTCCTA GCCAGGCACT GCTGCCCCTC CCTAGGAATG 
    TGGGCCTCAG AGGCCGGCAG TGTGAGGTTC TCTGTGCCGC CCTCCATCAG CAGGAACCGG
    GCCATGGTGA ATGAACCCAG AGGGAATGGT GGCCCCATCC CCCGCTCGGA TGGCAGCAGC
    AGTGGCAGCG AGAGCTCCAG AGATAGTTCA AGATGTTCCA CCCCGGGGCT GGACCCCGAG
    CGGCACGAGA GACTCAGGGA GAAGATGAGG CGGAGGATGG AGTCTGGTGA CAAGTGGTTC
    TCCCTAGAAT TCTTCCCTCC TCGGACTGCC CAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCAGATGG GGGCTGGTGG CCCCCTCTTC ATAGATGTGA CCTGGCACCC AGCAGGGGAC
    CCCGGCTCGG ACAAGGAGAC CTCCTCTATG ATGATTGCCA GCACTGCTGT GAACTACTGT
    GGCCTGGAGA CCGTCCTGCA CATGACCTGC TGCCGCCAGA GCCGGGAAGA GATCACCGGC
    TATCTGCACA AGGCCAAGCG GCTGGGCCTG AAGAACATCT TGGCGTTGAG GGGAGACCCT
    GTAGGTGACC AGTGGGAAGA GGAGGAAGGT GGCTTCAGCT ATGCTGCAGA CTTGGTGAAG
    CATATCCGGA GCGAGTTCGG TGACTACTTT GACGTCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCTGACT CGGGGAGCTT TGAGGCCGAC CTGAAGTACT TGAAGGAGAA GGTGTCTGCA
    GGAGCCGACT TCATCATCAC TCAGCTTTTC TTTGAGGCTG ACACGTTCTT CCACTTTGTT
    AAGGCTTGCT CCGAGATAGG CATTACCTGC CCCATCCTCC CCGGCATCTT TCCTATTCAG
    GGCTACCACT CCCTTCGGCA GCTGGTGAAG CTGTCGAAGC TGGAAGTGCC GCAGGAGATC
    AAAGACGTGA TCGAGCCAAT CAAAGACAAC GACGGCGCCA TCCGCAACTA TGGCATCGAC
    CAGGCCGTGA GCCTGTGCCA GCAGCTGCTG GCAAGTGGCT TGGTGCCAGG CCTCCACTTC
    TATACCCTCA ACCGTGAGGT GGCCACCACA GAGGTGCTGA AGCGCCTGGG CATGTGGGTC
    GAGGACCCCA GGCGCCCCCT GCCCTGGGCT CTCAGTGCCC ACCCCAAGCG CCGGGAGGAA
    GATGTCCGTC CCATCTTCTG GGCCACCAGA CCCAAGAGTT ACATCTACCG CACCCAGGAG
    TGGGATGAGT TCCCCAACGG CCGCTGGGGC AATTCCTCCT CGCCCGCCTT CGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCGA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG CGAGGAGAGC GTCTTCGAAG TCTTTGCCCA CTACCTCTCG
    GGAGAGCCCA ACCAGAACGG CTATAAAGTG ACTTGCCTGC CCTGGAATGA TGAGCCCTTG
    GCTGCCGAGA CGAGCCTGAT GAAGGAGGAG CTGCTGCGAG TGAACCGGCA GGGCGTCCTC
    ACCATCAACT CCCAGCCCAA CATCAATGGG AAGCCGTCCT CCGACCCCGT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTCTTCAC TTCCCGCGAG
    ACAGTGGAAG CGCTGCTGCA GGTGCTGAAG AAATATGAGC TCCGGGTCAA CTACCACATC
    GTGGACGTGA AGGGTGAAAA CATCACCAAT GCTCCTGAGC TGCAGCCCAA CGCCGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACGG TGGTGGATCC CATCAGCTTC
    ATGTTCTGGA AGGATGAGGC CTTCGCCCTG TGGATTGAGC AGTGGGGCAA GCTGTACGAG
    GAGGAGTCCC CGTCCCGCAT GATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTTCC GCTGGACAAC TGCCTGTGGC AGGTGGTAGA AGACACATTT
    GAGCTTCTCA ACAGGCCTGC CCCGAATGAG AGGGAGATGG AGGCTCCGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb29377
    Clone ID Related Accession (Same CDS sequence) XM_014737646.1
    Accession Version XM_014737646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867403.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCCCTTG CCCCCACCCC GTGCAGCAGG AACCGGGCCA TGGTGAATGA ACCCAGAGGG 
    AATGGTGGCC CCATCCCCCG CTCGGATGGC AGCAGCAGTG GCAGCGAGAG CTCCAGAGAT
    AGTTCAAGAT GTTCCACCCC GGGGCTGGAC CCCGAGCGGC ACGAGAGACT CAGGGAGAAG
    ATGAGGCGGA GGATGGAGTC TGGTGACAAG TGGTTCTCCC TAGAATTCTT CCCTCCTCGG
    ACTGCCCAGG GAGCTGTCAA TCTCATCTCA AGGTTTGACC AGATGGGGGC TGGTGGCCCC
    CTCTTCATAG ATGTGACCTG GCACCCAGCA GGGGACCCCG GCTCGGACAA GGAGACCTCC
    TCTATGATGA TTGCCAGCAC TGCTGTGAAC TACTGTGGCC TGGAGACCGT CCTGCACATG
    ACCTGCTGCC GCCAGAGCCG GGAAGAGATC ACCGGCTATC TGCACAAGGC CAAGCGGCTG
    GGCCTGAAGA ACATCTTGGC GTTGAGGGGA GACCCTGTAG GTGACCAGTG GGAAGAGGAG
    GAAGGTGGCT TCAGCTATGC TGCAGACTTG GTGAAGCATA TCCGGAGCGA GTTCGGTGAC
    TACTTTGACG TCTGTGTGGC AGGTTACCCC AAAGGCCACC CTGACTCGGG GAGCTTTGAG
    GCCGACCTGA AGTACTTGAA GGAGAAGGTG TCTGCAGGAG CCGACTTCAT CATCACTCAG
    CTTTTCTTTG AGGCTGACAC GTTCTTCCAC TTTGTTAAGG CTTGCTCCGA GATAGGCATT
    ACCTGCCCCA TCCTCCCCGG CATCTTTCCT ATTCAGGGCT ACCACTCCCT TCGGCAGCTG
    GTGAAGCTGT CGAAGCTGGA AGTGCCGCAG GAGATCAAAG ACGTGATCGA GCCAATCAAA
    GACAACGACG GCGCCATCCG CAACTATGGC ATCGACCAGG CCGTGAGCCT GTGCCAGCAG
    CTGCTGGCAA GTGGCTTGGT GCCAGGCCTC CACTTCTATA CCCTCAACCG TGAGGTGGCC
    ACCACAGAGG TGCTGAAGCG CCTGGGCATG TGGGTCGAGG ACCCCAGGCG CCCCCTGCCC
    TGGGCTCTCA GTGCCCACCC CAAGCGCCGG GAGGAAGATG TCCGTCCCAT CTTCTGGGCC
    ACCAGACCCA AGAGTTACAT CTACCGCACC CAGGAGTGGG ATGAGTTCCC CAACGGCCGC
    TGGGGCAATT CCTCCTCGCC CGCCTTCGGG GAGCTGAAGG ACTACTACCT CTTCTACCTG
    AAGAGCAAGT CCCCGAAGGA GGAGCTGCTG AAGATGTGGG GGGAGGAGCT GACCAGCGAG
    GAGAGCGTCT TCGAAGTCTT TGCCCACTAC CTCTCGGGAG AGCCCAACCA GAACGGCTAT
    AAAGTGACTT GCCTGCCCTG GAATGATGAG CCCTTGGCTG CCGAGACGAG CCTGATGAAG
    GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC GTCCTCACCA TCAACTCCCA GCCCAACATC
    AATGGGAAGC CGTCCTCCGA CCCCGTCGTG GGCTGGGGCC CCAGCGGGGG CTATGTCTTC
    CAGAAGGCCT ACTTAGAGTT CTTCACTTCC CGCGAGACAG TGGAAGCGCT GCTGCAGGTG
    CTGAAGAAAT ATGAGCTCCG GGTCAACTAC CACATCGTGG ACGTGAAGGG TGAAAACATC
    ACCAATGCTC CTGAGCTGCA GCCCAACGCC GTCACTTGGG GCATCTTCCC TGGGCGAGAG
    ATCATCCAGC CCACGGTGGT GGATCCCATC AGCTTCATGT TCTGGAAGGA TGAGGCCTTC
    GCCCTGTGGA TTGAGCAGTG GGGCAAGCTG TACGAGGAGG AGTCCCCGTC CCGCATGATC
    ATCCAGTACA TCCACGACAA CTACTTCCTG GTCAACCTGG TGGACAATGA CTTTCCGCTG
    GACAACTGCC TGTGGCAGGT GGTAGAAGAC ACATTTGAGC TTCTCAACAG GCCTGCCCCG
    AATGAGAGGG AGATGGAGGC TCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014593132.1
    CDS1815..3821
    Translation

    Target ORF information:

    RefSeq Version XM_014737646.1
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014737646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCCCTTG CCCCCACCCC GTGCAGCAGG AACCGGGCCA TGGTGAATGA ACCCAGAGGG 
    AATGGTGGCC CCATCCCCCG CTCGGATGGC AGCAGCAGTG GCAGCGAGAG CTCCAGAGAT
    AGTTCAAGAT GTTCCACCCC GGGGCTGGAC CCCGAGCGGC ACGAGAGACT CAGGGAGAAG
    ATGAGGCGGA GGATGGAGTC TGGTGACAAG TGGTTCTCCC TAGAATTCTT CCCTCCTCGG
    ACTGCCCAGG GAGCTGTCAA TCTCATCTCA AGGTTTGACC AGATGGGGGC TGGTGGCCCC
    CTCTTCATAG ATGTGACCTG GCACCCAGCA GGGGACCCCG GCTCGGACAA GGAGACCTCC
    TCTATGATGA TTGCCAGCAC TGCTGTGAAC TACTGTGGCC TGGAGACCGT CCTGCACATG
    ACCTGCTGCC GCCAGAGCCG GGAAGAGATC ACCGGCTATC TGCACAAGGC CAAGCGGCTG
    GGCCTGAAGA ACATCTTGGC GTTGAGGGGA GACCCTGTAG GTGACCAGTG GGAAGAGGAG
    GAAGGTGGCT TCAGCTATGC TGCAGACTTG GTGAAGCATA TCCGGAGCGA GTTCGGTGAC
    TACTTTGACG TCTGTGTGGC AGGTTACCCC AAAGGCCACC CTGACTCGGG GAGCTTTGAG
    GCCGACCTGA AGTACTTGAA GGAGAAGGTG TCTGCAGGAG CCGACTTCAT CATCACTCAG
    CTTTTCTTTG AGGCTGACAC GTTCTTCCAC TTTGTTAAGG CTTGCTCCGA GATAGGCATT
    ACCTGCCCCA TCCTCCCCGG CATCTTTCCT ATTCAGGGCT ACCACTCCCT TCGGCAGCTG
    GTGAAGCTGT CGAAGCTGGA AGTGCCGCAG GAGATCAAAG ACGTGATCGA GCCAATCAAA
    GACAACGACG GCGCCATCCG CAACTATGGC ATCGACCAGG CCGTGAGCCT GTGCCAGCAG
    CTGCTGGCAA GTGGCTTGGT GCCAGGCCTC CACTTCTATA CCCTCAACCG TGAGGTGGCC
    ACCACAGAGG TGCTGAAGCG CCTGGGCATG TGGGTCGAGG ACCCCAGGCG CCCCCTGCCC
    TGGGCTCTCA GTGCCCACCC CAAGCGCCGG GAGGAAGATG TCCGTCCCAT CTTCTGGGCC
    ACCAGACCCA AGAGTTACAT CTACCGCACC CAGGAGTGGG ATGAGTTCCC CAACGGCCGC
    TGGGGCAATT CCTCCTCGCC CGCCTTCGGG GAGCTGAAGG ACTACTACCT CTTCTACCTG
    AAGAGCAAGT CCCCGAAGGA GGAGCTGCTG AAGATGTGGG GGGAGGAGCT GACCAGCGAG
    GAGAGCGTCT TCGAAGTCTT TGCCCACTAC CTCTCGGGAG AGCCCAACCA GAACGGCTAT
    AAAGTGACTT GCCTGCCCTG GAATGATGAG CCCTTGGCTG CCGAGACGAG CCTGATGAAG
    GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC GTCCTCACCA TCAACTCCCA GCCCAACATC
    AATGGGAAGC CGTCCTCCGA CCCCGTCGTG GGCTGGGGCC CCAGCGGGGG CTATGTCTTC
    CAGAAGGCCT ACTTAGAGTT CTTCACTTCC CGCGAGACAG TGGAAGCGCT GCTGCAGGTG
    CTGAAGAAAT ATGAGCTCCG GGTCAACTAC CACATCGTGG ACGTGAAGGG TGAAAACATC
    ACCAATGCTC CTGAGCTGCA GCCCAACGCC GTCACTTGGG GCATCTTCCC TGGGCGAGAG
    ATCATCCAGC CCACGGTGGT GGATCCCATC AGCTTCATGT TCTGGAAGGA TGAGGCCTTC
    GCCCTGTGGA TTGAGCAGTG GGGCAAGCTG TACGAGGAGG AGTCCCCGTC CCGCATGATC
    ATCCAGTACA TCCACGACAA CTACTTCCTG GTCAACCTGG TGGACAATGA CTTTCCGCTG
    GACAACTGCC TGTGGCAGGT GGTAGAAGAC ACATTTGAGC TTCTCAACAG GCCTGCCCCG
    AATGAGAGGG AGATGGAGGC TCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb29378
    Clone ID Related Accession (Same CDS sequence) XM_014737647.1
    Accession Version XM_014737647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1746bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product methylenetetrahydrofolate reductase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867403.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCTTG CCCCCACCCC GTGCAGCAGG AACCGGGCCA TGGTGAATGA ACCCAGAGGG 
    AATGGTGGCC CCATCCCCCG CTCGGATGGC AGCAGCAGTG GCAGCGAGAG CTCCAGAGAT
    AGTTCAAGAT GTTCCACCCC GGGGCTGGAC CCCGAGCGGC ACGAGAGACT CAGGGAGAAG
    ATGAGGCGGA GGATGGAGTC TGGTGACAAG TGGTTCTCCC TAGAATTCTT CCCTCCTCGG
    ACTGCCCAGG GAGCTGTCAA TCTCATCTCA AGGTTTGACC AGATGGGGGC TGGTGGCCCC
    CTCTTCATAG ATGTGACCTG GCACCCAGCA GGGGACCCCG GCTCGGACAA GGAGACCTCC
    TCTATGATGA TTGCCAGCAC TGCTGTGAAC TACTGTGGCC TGGAGACCGT CCTGCACATG
    ACCTGCTGCC GCCAGAGCCG GGAAGAGATC ACCGGCTATC TGCACAAGGC CAAGCGGCTG
    GGCCTGAAGA ACATCTTGGC GTTGAGGGGA GACCCTGTAG GTGACCAGTG GGAAGAGGAG
    GAAGGTGGCT TCAGCTATGC TGCAGACTTG GTGAAGCATA TCCGGAGCGA GTTCGGTGAC
    TACTTTGACG TCTGTGTGGC AGGTTACCCC AAAGGCCACC CTGACTCGGG GAGCTTTGAG
    GCCGACCTGA AGTACTTGAA GGAGAAGGTG TCTGCAGGAG CCGACTTCAT CATCACTCAG
    CTTTTCTTTG AGGCTGACAC GTTCTTCCAC TTTGTTAAGG CTTGCTCCGA GATAGGCATT
    ACCTGCCCCA TCCTCCCCGG CATCTTTCCT ATTCAGGGCT ACCACTCCCT TCGGCAGCTG
    GTGAAGCTGT CGAAGCTGGA AGTGCCGCAG GAGATCAAAG ACGTGATCGA GCCAATCAAA
    GACAACGACG GCGCCATCCG CAACTATGGC ATCGACCAGG CCGTGAGCCT GTGCCAGCAG
    CTGCTGGCAA GTGGCTTGGT GCCAGGCCTC CACTTCTATA CCCTCAACCG TGAGGTGGCC
    ACCACAGAGG TGCTGAAGCG CCTGGGCATG TGGGTCGAGG ACCCCAGGCG CCCCCTGCCC
    TGGGCTCTCA GTGCCCACCC CAAGCGCCGG GAGGAAGATG TCCGTCCCAT CTTCTGGGCC
    ACCAGACCCA AGAGTTACAT CTACCGCACC CAGGAGTGGG ATGAGTTCCC CAACGGCCGC
    TGGGGCAATT CCTCCTCGCC CGCCTTCGGG GAGCTGAAGG ACTACTACCT CTTCTACCTG
    AAGAGCAAGT CCCCGAAGGA GGAGCTGCTG AAGATGTGGG GGGAGGAGCT GACCAGCGAG
    GAGAGCGTCT TCGAAGTCTT TGCCCACTAC CTCTCGGGAG AGCCCAACCA GAACGGCTAT
    AAAGTGACTT GCCTGCCCTG GAATGATGAG CCCTTGGCTG CCGAGACGAG CCTGATGAAG
    GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC GTCCTCACCA TCAACTCCCA GCCCAACATC
    AATGGGAAGC CGTCCTCCGA CCCCGTCGTG GGCTGGGGCC CCAGCGGGGG CTATGTCTTC
    CAGAAGGCCT ACTTAGAGTT CTTCACTTCC CGCGAGACAG TGGAAGCGCT GCTGCAGGTG
    CTGAAGAAAT ATGAGCTCCG GGTCAACTAC CACATCGTGG ACGTGAAGGC AGGTGACAGA
    GGACAGCTGG GGCCGGAGAA GACTAGAATC AGAGGCCCTT TTAGTCTGAC TAGCAGAGAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014593133.1
    CDS91..1836
    Translation

    Target ORF information:

    RefSeq Version XM_014737647.1
    Organism Equus caballus(horse)
    Definition Equus caballus methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014737647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGCCCCTTG CCCCCACCCC GTGCAGCAGG AACCGGGCCA TGGTGAATGA ACCCAGAGGG 
    AATGGTGGCC CCATCCCCCG CTCGGATGGC AGCAGCAGTG GCAGCGAGAG CTCCAGAGAT
    AGTTCAAGAT GTTCCACCCC GGGGCTGGAC CCCGAGCGGC ACGAGAGACT CAGGGAGAAG
    ATGAGGCGGA GGATGGAGTC TGGTGACAAG TGGTTCTCCC TAGAATTCTT CCCTCCTCGG
    ACTGCCCAGG GAGCTGTCAA TCTCATCTCA AGGTTTGACC AGATGGGGGC TGGTGGCCCC
    CTCTTCATAG ATGTGACCTG GCACCCAGCA GGGGACCCCG GCTCGGACAA GGAGACCTCC
    TCTATGATGA TTGCCAGCAC TGCTGTGAAC TACTGTGGCC TGGAGACCGT CCTGCACATG
    ACCTGCTGCC GCCAGAGCCG GGAAGAGATC ACCGGCTATC TGCACAAGGC CAAGCGGCTG
    GGCCTGAAGA ACATCTTGGC GTTGAGGGGA GACCCTGTAG GTGACCAGTG GGAAGAGGAG
    GAAGGTGGCT TCAGCTATGC TGCAGACTTG GTGAAGCATA TCCGGAGCGA GTTCGGTGAC
    TACTTTGACG TCTGTGTGGC AGGTTACCCC AAAGGCCACC CTGACTCGGG GAGCTTTGAG
    GCCGACCTGA AGTACTTGAA GGAGAAGGTG TCTGCAGGAG CCGACTTCAT CATCACTCAG
    CTTTTCTTTG AGGCTGACAC GTTCTTCCAC TTTGTTAAGG CTTGCTCCGA GATAGGCATT
    ACCTGCCCCA TCCTCCCCGG CATCTTTCCT ATTCAGGGCT ACCACTCCCT TCGGCAGCTG
    GTGAAGCTGT CGAAGCTGGA AGTGCCGCAG GAGATCAAAG ACGTGATCGA GCCAATCAAA
    GACAACGACG GCGCCATCCG CAACTATGGC ATCGACCAGG CCGTGAGCCT GTGCCAGCAG
    CTGCTGGCAA GTGGCTTGGT GCCAGGCCTC CACTTCTATA CCCTCAACCG TGAGGTGGCC
    ACCACAGAGG TGCTGAAGCG CCTGGGCATG TGGGTCGAGG ACCCCAGGCG CCCCCTGCCC
    TGGGCTCTCA GTGCCCACCC CAAGCGCCGG GAGGAAGATG TCCGTCCCAT CTTCTGGGCC
    ACCAGACCCA AGAGTTACAT CTACCGCACC CAGGAGTGGG ATGAGTTCCC CAACGGCCGC
    TGGGGCAATT CCTCCTCGCC CGCCTTCGGG GAGCTGAAGG ACTACTACCT CTTCTACCTG
    AAGAGCAAGT CCCCGAAGGA GGAGCTGCTG AAGATGTGGG GGGAGGAGCT GACCAGCGAG
    GAGAGCGTCT TCGAAGTCTT TGCCCACTAC CTCTCGGGAG AGCCCAACCA GAACGGCTAT
    AAAGTGACTT GCCTGCCCTG GAATGATGAG CCCTTGGCTG CCGAGACGAG CCTGATGAAG
    GAGGAGCTGC TGCGAGTGAA CCGGCAGGGC GTCCTCACCA TCAACTCCCA GCCCAACATC
    AATGGGAAGC CGTCCTCCGA CCCCGTCGTG GGCTGGGGCC CCAGCGGGGG CTATGTCTTC
    CAGAAGGCCT ACTTAGAGTT CTTCACTTCC CGCGAGACAG TGGAAGCGCT GCTGCAGGTG
    CTGAAGAAAT ATGAGCTCCG GGTCAACTAC CACATCGTGG ACGTGAAGGC AGGTGACAGA
    GGACAGCTGG GGCCGGAGAA GACTAGAATC AGAGGCCCTT TTAGTCTGAC TAGCAGAGAG
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.