GLI1 cDNA ORF clone, Equus caballus(horse)

The following GLI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb00157 NM_001163860.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66004 NM_001163860.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb00157 XM_014740744.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb00157 XM_014740743.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb00157 XM_014740741.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb00157 XM_014740742.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb22361 XM_014740745.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb31353 XM_014740746.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66004 XM_014740741.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66004 XM_014740743.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66004 XM_014740744.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66004 XM_014740742.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66004 XM_023642154.1
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66005 XM_014740745.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb66006 XM_014740746.2
Latest version!
Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb00157
    Clone ID Related Accession (Same CDS sequence) XM_014740741.1 , XM_014740742.1 , XM_014740744.1 , XM_014740743.1 , NM_001163860.1
    Accession Version NM_001163860.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAWR02031382.1. On Aug 6, 2009 this sequence version replaced XM_001488805.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2743238, SAMN03263062 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157332.1
    CDS79..3399
    Translation

    Target ORF information:

    RefSeq Version NM_001163860.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66004
    Clone ID Related Accession (Same CDS sequence) NM_001163860.2 , XM_014740742.2 , XM_023642154.1 , XM_014740741.2 , XM_014740743.2 , XM_014740744.2
    Accession Version NM_001163860.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-27
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from PJAA01000007.1. On Jun 28, 2018 this sequence version replaced NM_001163860.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: GDHK01064098.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2743238, SAMN05772704 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157332.2
    CDS79..3399
    Translation

    Target ORF information:

    RefSeq Version NM_001163860.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163860.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb00157
    Clone ID Related Accession (Same CDS sequence) XM_014740741.1 , XM_014740742.1 , XM_014740744.1 , XM_014740743.1 , NM_001163860.1
    Accession Version XM_014740741.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596227.1
    CDS327..3647
    Translation

    Target ORF information:

    RefSeq Version XM_014740741.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740741.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb00157
    Clone ID Related Accession (Same CDS sequence) XM_014740741.1 , XM_014740742.1 , XM_014740744.1 , XM_014740743.1 , NM_001163860.1
    Accession Version XM_014740742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596228.1
    CDS246..3566
    Translation

    Target ORF information:

    RefSeq Version XM_014740742.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb00157
    Clone ID Related Accession (Same CDS sequence) XM_014740741.1 , XM_014740742.1 , XM_014740744.1 , XM_014740743.1 , NM_001163860.1
    Accession Version XM_014740744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596230.1
    CDS181..3501
    Translation

    Target ORF information:

    RefSeq Version XM_014740744.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb00157
    Clone ID Related Accession (Same CDS sequence) XM_014740741.1 , XM_014740742.1 , XM_014740744.1 , XM_014740743.1 , NM_001163860.1
    Accession Version XM_014740743.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596229.1
    CDS306..3626
    Translation

    Target ORF information:

    RefSeq Version XM_014740743.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740743.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCTGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb22361
    Clone ID Related Accession (Same CDS sequence) XM_014740745.1
    Accession Version XM_014740745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGAAGCC ACAGCCAAGC CCTGGGGCCC AGTCATCCTG CAGCAGTGAC
    CACTCCCCAG CAGGCAGCGC AGCCAATACA GACAGTGGTG TGGAAATGAC TGGAAATGCA
    GGGGGCAGCA CCGAGGACCT GTCTAGCTTG GACGAGGGGC CTTGCATTGC TGGCACTGGT
    CTGTCCACTC TTCGCCGCCT TGAGAACCTC AGGCTGGACC AGCTACATCA ACTCTGGCCA
    GGAGGGCCCC GGGGCCTCAA ACTGCCCAGC TTGACCCATA CGGGCACCCC TGCGTCCCGC
    CGTCTGGGCC CTCCAGCTTC TCTTGACCGC CGTAGCAGCA GCTCCAGCAG TGTCAGCTCG
    GCCTATACTG TCAGCCGCCG CTCCTCCCTG GCCTCCCCTT TCCCGCCTGG CTCCCCACCA
    GAGAATGGGG CATCCTCGCT GCCTGGCCTC ACGCCTGCCC AGCACTACCT GCTCCGGGCA
    AGATATGCTT CAGCCAGGGG AGGTGGTACC CCACCCACTG CAGCACCCAG CCTGGATCGG
    ATGGGGGGTC TTCCTGCACC TCCCTGGAGA AGCCGAGCTG AGTATCCAGG TTACAACCCC
    AACGTAGGAG TCACCCGGAG GGCCAGTGAC CCAGCCCGGG CTGCTGACCG CCCTGCCCCG
    GCCAGAGTCC AGCGGTTCAA GAGCTTGGGT TGTGTCCACA CACCCCCTAC CATGACAGGG
    GGAGGACCGA ACTTTGACCC CCAACTCTCG ACCTCTGTCT ACTCACCACA GCCCCCCAGC
    ATCACTGAGA ATATTGCCAT GGATACCAGA GGGCTACGGG AGGAGCCAGA GATTGGGACC
    TCCATGATGG GCAGTGGTCT GAATCCCTAT ATGGACTTCC CATCTACTGA TACTCTGGGA
    TATGGGGGAC CTGAGGGGGC AGCAGCTGAG CCTTATGGAG CTAGGGGTCC AGGCTCTCTT
    CCTCTTGGAC CTGGTCCACC CACCAACTAT GGCCCCAATC CCTGTCCCCA GCAGGTCTCC
    TATCCTGACC CCACTCCAGA AACATGGGGT GAGTTCCCTT CCCACACCGG GCTGTACCCA
    GGCCCCAAGC CTCCGGTTGG AGCCTACAGC CAATGTCCTC GTCTTGAACA TTATGGACAA
    GTGCAAGTCA AGCCAGAACA GGGGTGTCCA GTGGGTTCTG ACTCCACAGG ACTGGCACCC
    TGCCTCAATG CCCATCCCAA TGAGGGGCCT CCACGACCAC AGCCTCTGTT CTCCCACTAC
    CCCCAGCCTC CCCCTCCCCA ATATCCCCAG GCAGGCCCCT ATCCCCAACC ACCCCCTGAT
    TACCTTCCTT CAGAATCCAG GCCTGGCCTG GATTTTGATT CCCCCACTCA TTCCACAGGA
    CAGCTCAAGG CTCAGCTCGT GTGTGATTAT GTCCAGTCTC AACAGGAGCT GCTGTGGGAG
    GGTGGGGGGA GAGGCGATCC CCCAGTCCAG GAACCTCTCT ACCAGAGTCC CAAGTTTCTG
    GGGGGTTCTC AGGTTAGCCC GAACCCTGCC AAGGCCCCAG TGGCCACCTA CGGACCTGGC
    TTTGCACCTA ACTTGCCCCA TCATAAGTCA GGCTCCTATC CCACCCCTTC ACCATGTCAT
    GAAAATTTTG CAGTGGGGGC AAACAAGTCT TCCCATCGGG CAGCAGCACC ACCCCGACTT
    CTGCCCCCAC TGCCAACTTG CTATGGGCCC CTCAAGGCAG GGGGAAGCAA CCCCAGCTGT
    GGCCATCCAG AGGTGGGCAG GTTGGGAGGG GGTCCTGCCT TATACCCTCC TCCTGAAGGA
    CAAGTGTGTA ACCCTCTGGA CTCTCTTGAT CTTGACAACA CTCAGCTGGA CTTTGTGGCT
    ATTCTGGATG AGGCCCAGGG GCTGAGTCCT CCCCCTGCCC ATGATCAGGG GGAGAGCTCT
    GAACACACCC CACCTCCGTC TGGGCCCCCC AACATGGCTG TGGGTAACAT GAGTGTGTTA
    CTGGGGTCCC TGCCTGGGGA GACACAATTC CTCAACTCTA GTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596231.1
    CDS327..3554
    Translation

    Target ORF information:

    RefSeq Version XM_014740745.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGAAGCC ACAGCCAAGC CCTGGGGCCC AGTCATCCTG CAGCAGTGAC
    CACTCCCCAG CAGGCAGCGC AGCCAATACA GACAGTGGTG TGGAAATGAC TGGAAATGCA
    GGGGGCAGCA CCGAGGACCT GTCTAGCTTG GACGAGGGGC CTTGCATTGC TGGCACTGGT
    CTGTCCACTC TTCGCCGCCT TGAGAACCTC AGGCTGGACC AGCTACATCA ACTCTGGCCA
    GGAGGGCCCC GGGGCCTCAA ACTGCCCAGC TTGACCCATA CGGGCACCCC TGCGTCCCGC
    CGTCTGGGCC CTCCAGCTTC TCTTGACCGC CGTAGCAGCA GCTCCAGCAG TGTCAGCTCG
    GCCTATACTG TCAGCCGCCG CTCCTCCCTG GCCTCCCCTT TCCCGCCTGG CTCCCCACCA
    GAGAATGGGG CATCCTCGCT GCCTGGCCTC ACGCCTGCCC AGCACTACCT GCTCCGGGCA
    AGATATGCTT CAGCCAGGGG AGGTGGTACC CCACCCACTG CAGCACCCAG CCTGGATCGG
    ATGGGGGGTC TTCCTGCACC TCCCTGGAGA AGCCGAGCTG AGTATCCAGG TTACAACCCC
    AACGTAGGAG TCACCCGGAG GGCCAGTGAC CCAGCCCGGG CTGCTGACCG CCCTGCCCCG
    GCCAGAGTCC AGCGGTTCAA GAGCTTGGGT TGTGTCCACA CACCCCCTAC CATGACAGGG
    GGAGGACCGA ACTTTGACCC CCAACTCTCG ACCTCTGTCT ACTCACCACA GCCCCCCAGC
    ATCACTGAGA ATATTGCCAT GGATACCAGA GGGCTACGGG AGGAGCCAGA GATTGGGACC
    TCCATGATGG GCAGTGGTCT GAATCCCTAT ATGGACTTCC CATCTACTGA TACTCTGGGA
    TATGGGGGAC CTGAGGGGGC AGCAGCTGAG CCTTATGGAG CTAGGGGTCC AGGCTCTCTT
    CCTCTTGGAC CTGGTCCACC CACCAACTAT GGCCCCAATC CCTGTCCCCA GCAGGTCTCC
    TATCCTGACC CCACTCCAGA AACATGGGGT GAGTTCCCTT CCCACACCGG GCTGTACCCA
    GGCCCCAAGC CTCCGGTTGG AGCCTACAGC CAATGTCCTC GTCTTGAACA TTATGGACAA
    GTGCAAGTCA AGCCAGAACA GGGGTGTCCA GTGGGTTCTG ACTCCACAGG ACTGGCACCC
    TGCCTCAATG CCCATCCCAA TGAGGGGCCT CCACGACCAC AGCCTCTGTT CTCCCACTAC
    CCCCAGCCTC CCCCTCCCCA ATATCCCCAG GCAGGCCCCT ATCCCCAACC ACCCCCTGAT
    TACCTTCCTT CAGAATCCAG GCCTGGCCTG GATTTTGATT CCCCCACTCA TTCCACAGGA
    CAGCTCAAGG CTCAGCTCGT GTGTGATTAT GTCCAGTCTC AACAGGAGCT GCTGTGGGAG
    GGTGGGGGGA GAGGCGATCC CCCAGTCCAG GAACCTCTCT ACCAGAGTCC CAAGTTTCTG
    GGGGGTTCTC AGGTTAGCCC GAACCCTGCC AAGGCCCCAG TGGCCACCTA CGGACCTGGC
    TTTGCACCTA ACTTGCCCCA TCATAAGTCA GGCTCCTATC CCACCCCTTC ACCATGTCAT
    GAAAATTTTG CAGTGGGGGC AAACAAGTCT TCCCATCGGG CAGCAGCACC ACCCCGACTT
    CTGCCCCCAC TGCCAACTTG CTATGGGCCC CTCAAGGCAG GGGGAAGCAA CCCCAGCTGT
    GGCCATCCAG AGGTGGGCAG GTTGGGAGGG GGTCCTGCCT TATACCCTCC TCCTGAAGGA
    CAAGTGTGTA ACCCTCTGGA CTCTCTTGAT CTTGACAACA CTCAGCTGGA CTTTGTGGCT
    ATTCTGGATG AGGCCCAGGG GCTGAGTCCT CCCCCTGCCC ATGATCAGGG GGAGAGCTCT
    GAACACACCC CACCTCCGTC TGGGCCCCCC AACATGGCTG TGGGTAACAT GAGTGTGTTA
    CTGGGGTCCC TGCCTGGGGA GACACAATTC CTCAACTCTA GTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb31353
    Clone ID Related Accession (Same CDS sequence) XM_014740746.1
    Accession Version XM_014740746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867424.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGCC CCCACAGTTA TGGGCCAGCC AGAGAGACCA ACAGCTGCAC TGAGGCCCCA 
    CTTTTTCCTC CTCCCCGGAG TGCAGTCAAG TTGACCAAGA AGCGGGCACT CTCCATCTCA
    CCCCTCTCGG ACGCCAGCCT GGACCTGCAG ACAGTTATCC GCACCTCGCC CAGCTCCCTT
    GTGGCCTTTA TCAACTCACG CTGTGCATCT CCGGGAGGCT CCTATGGTCA CCTCTCCATC
    AGCACCATGA GCCCATCTCT GGGATTCCCA GCCCAGATGA ATCACCAAAA AGGGACCTCG
    CCTTCCTTTG CGGTCCAGCC CTGTGGTCCC CATGACCCCA CCCACAGTGG GATGATGCCG
    CATCCTCAGT CCCGGGGACC CCTCCCAACT TGCCAGCTGA AGTCTGAGCT GGACATGCTG
    GTTAGCAAAT GCCCCGAGGA GCCCTTGGAA GGTGATATGT CCAGCCCCAA CTCCACTGGC
    ACACAGGATC CCCTGCTGGG GCTGCTGGAT GGGCGGGAGG ATCTGGAGCG AGAAGAGAAG
    CCCGAGCCTG AGTCTGTGTA TGAGACTGAC TGCCGCTGGG ATGGCTGCAG CCAGGAGTTT
    GACTCCCAGG AGCAGCTAGT GCACCACATC AACAGCGAGC ACATCCACGG GGAGCGGAAG
    GAGTTCGTGT GCCATTGGGG GGGCTGCTCC CGGGAGCTGA GGCCCTTCAA AGCTCAGTAC
    ATGCTGGTGG TCCACATGCG CAGACACACG GGCGAGAAGC CACACAAGTG CACGTTTGAG
    GGGTGCCGGA AGTCATACTC ACGCCTCGAA AATCTGAAGA CGCACCTGCG GTCCCACACG
    GGTGAGAAGC CGTACATGTG TGAGCATGAG GGCTGCAGCA AGGCCTTCAG CAATGCCAGT
    GACCGGGCCA AGCACCAGAA CCGGACCCAC TCCAATGAGA AGCCGTACGT GTGTAAGCTC
    CCCGGCTGTA CTAAACGCTA CACAGATCCC AGCTCGCTCC GAAAACACGT CAAGACGGTC
    CATGGTCCTG ATGCCCATGT GACCAAGCGG CACCGAGGCG ACGGCCCCCT ACCCCGGGTA
    CCATCCCTTT CCACAGTGGA GCCCAAGAGG GAGCGGGATG GAGGCCCCAT CAGGGAAGAG
    AGCAGACTGG CCGTGCCGGA GGGGGCCATG AAGCCACAGC CAAGCCCTGG GGCCCAGTCA
    TCCTGCAGCA GTGACCACTC CCCAGCAGGC AGCGCAGCCA ATACAGACAG TGGTGTGGAA
    ATGACTGGAA ATGCAGGGGG CAGCACCGAG GACCTGTCTA GCTTGGACGA GGGGCCTTGC
    ATTGCTGGCA CTGGTCTGTC CACTCTTCGC CGCCTTGAGA ACCTCAGGCT GGACCAGCTA
    CATCAACTCT GGCCAGGAGG GCCCCGGGGC CTCAAACTGC CCAGCTTGAC CCATACGGGC
    ACCCCTGCGT CCCGCCGTCT GGGCCCTCCA GCTTCTCTTG ACCGCCGTAG CAGCAGCTCC
    AGCAGTGTCA GCTCGGCCTA TACTGTCAGC CGCCGCTCCT CCCTGGCCTC CCCTTTCCCG
    CCTGGCTCCC CACCAGAGAA TGGGGCATCC TCGCTGCCTG GCCTCACGCC TGCCCAGCAC
    TACCTGCTCC GGGCAAGATA TGCTTCAGCC AGGGGAGGTG GTACCCCACC CACTGCAGCA
    CCCAGCCTGG ATCGGATGGG GGGTCTTCCT GCACCTCCCT GGAGAAGCCG AGCTGAGTAT
    CCAGGTTACA ACCCCAACGT AGGAGTCACC CGGAGGGCCA GTGACCCAGC CCGGGCTGCT
    GACCGCCCTG CCCCGGCCAG AGTCCAGCGG TTCAAGAGCT TGGGTTGTGT CCACACACCC
    CCTACCATGA CAGGGGGAGG ACCGAACTTT GACCCCCAAC TCTCGACCTC TGTCTACTCA
    CCACAGCCCC CCAGCATCAC TGAGAATATT GCCATGGATA CCAGAGGGCT ACGGGAGGAG
    CCAGAGATTG GGACCTCCAT GATGGGCAGT GGTCTGAATC CCTATATGGA CTTCCCATCT
    ACTGATACTC TGGGATATGG GGGACCTGAG GGGGCAGCAG CTGAGCCTTA TGGAGCTAGG
    GGTCCAGGCT CTCTTCCTCT TGGACCTGGT CCACCCACCA ACTATGGCCC CAATCCCTGT
    CCCCAGCAGG TCTCCTATCC TGACCCCACT CCAGAAACAT GGGGTGAGTT CCCTTCCCAC
    ACCGGGCTGT ACCCAGGCCC CAAGCCTCCG GTTGGAGCCT ACAGCCAATG TCCTCGTCTT
    GAACATTATG GACAAGTGCA AGTCAAGCCA GAACAGGGGT GTCCAGTGGG TTCTGACTCC
    ACAGGACTGG CACCCTGCCT CAATGCCCAT CCCAATGAGG GGCCTCCACG ACCACAGCCT
    CTGTTCTCCC ACTACCCCCA GCCTCCCCCT CCCCAATATC CCCAGGCAGG CCCCTATCCC
    CAACCACCCC CTGATTACCT TCCTTCAGAA TCCAGGCCTG GCCTGGATTT TGATTCCCCC
    ACTCATTCCA CAGGACAGCT CAAGGCTCAG CTCGTGTGTG ATTATGTCCA GTCTCAACAG
    GAGCTGCTGT GGGAGGGTGG GGGGAGAGGC GATCCCCCAG TCCAGGAACC TCTCTACCAG
    AGTCCCAAGT TTCTGGGGGG TTCTCAGGTT AGCCCGAACC CTGCCAAGGC CCCAGTGGCC
    ACCTACGGAC CTGGCTTTGC ACCTAACTTG CCCCATCATA AGTCAGGCTC CTATCCCACC
    CCTTCACCAT GTCATGAAAA TTTTGCAGTG GGGGCAAACA AGTCTTCCCA TCGGGCAGCA
    GCACCACCCC GACTTCTGCC CCCACTGCCA ACTTGCTATG GGCCCCTCAA GGCAGGGGGA
    AGCAACCCCA GCTGTGGCCA TCCAGAGGTG GGCAGGTTGG GAGGGGGTCC TGCCTTATAC
    CCTCCTCCTG AAGGACAAGT GTGTAACCCT CTGGACTCTC TTGATCTTGA CAACACTCAG
    CTGGACTTTG TGGCTATTCT GGATGAGGCC CAGGGGCTGA GTCCTCCCCC TGCCCATGAT
    CAGGGGGAGA GCTCTGAACA CACCCCACCT CCGTCTGGGC CCCCCAACAT GGCTGTGGGT
    AACATGAGTG TGTTACTGGG GTCCCTGCCT GGGGAGACAC AATTCCTCAA CTCTAGTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596232.1
    CDS317..3499
    Translation

    Target ORF information:

    RefSeq Version XM_014740746.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGCC CCCACAGTTA TGGGCCAGCC AGAGAGACCA ACAGCTGCAC TGAGGCCCCA 
    CTTTTTCCTC CTCCCCGGAG TGCAGTCAAG TTGACCAAGA AGCGGGCACT CTCCATCTCA
    CCCCTCTCGG ACGCCAGCCT GGACCTGCAG ACAGTTATCC GCACCTCGCC CAGCTCCCTT
    GTGGCCTTTA TCAACTCACG CTGTGCATCT CCGGGAGGCT CCTATGGTCA CCTCTCCATC
    AGCACCATGA GCCCATCTCT GGGATTCCCA GCCCAGATGA ATCACCAAAA AGGGACCTCG
    CCTTCCTTTG CGGTCCAGCC CTGTGGTCCC CATGACCCCA CCCACAGTGG GATGATGCCG
    CATCCTCAGT CCCGGGGACC CCTCCCAACT TGCCAGCTGA AGTCTGAGCT GGACATGCTG
    GTTAGCAAAT GCCCCGAGGA GCCCTTGGAA GGTGATATGT CCAGCCCCAA CTCCACTGGC
    ACACAGGATC CCCTGCTGGG GCTGCTGGAT GGGCGGGAGG ATCTGGAGCG AGAAGAGAAG
    CCCGAGCCTG AGTCTGTGTA TGAGACTGAC TGCCGCTGGG ATGGCTGCAG CCAGGAGTTT
    GACTCCCAGG AGCAGCTAGT GCACCACATC AACAGCGAGC ACATCCACGG GGAGCGGAAG
    GAGTTCGTGT GCCATTGGGG GGGCTGCTCC CGGGAGCTGA GGCCCTTCAA AGCTCAGTAC
    ATGCTGGTGG TCCACATGCG CAGACACACG GGCGAGAAGC CACACAAGTG CACGTTTGAG
    GGGTGCCGGA AGTCATACTC ACGCCTCGAA AATCTGAAGA CGCACCTGCG GTCCCACACG
    GGTGAGAAGC CGTACATGTG TGAGCATGAG GGCTGCAGCA AGGCCTTCAG CAATGCCAGT
    GACCGGGCCA AGCACCAGAA CCGGACCCAC TCCAATGAGA AGCCGTACGT GTGTAAGCTC
    CCCGGCTGTA CTAAACGCTA CACAGATCCC AGCTCGCTCC GAAAACACGT CAAGACGGTC
    CATGGTCCTG ATGCCCATGT GACCAAGCGG CACCGAGGCG ACGGCCCCCT ACCCCGGGTA
    CCATCCCTTT CCACAGTGGA GCCCAAGAGG GAGCGGGATG GAGGCCCCAT CAGGGAAGAG
    AGCAGACTGG CCGTGCCGGA GGGGGCCATG AAGCCACAGC CAAGCCCTGG GGCCCAGTCA
    TCCTGCAGCA GTGACCACTC CCCAGCAGGC AGCGCAGCCA ATACAGACAG TGGTGTGGAA
    ATGACTGGAA ATGCAGGGGG CAGCACCGAG GACCTGTCTA GCTTGGACGA GGGGCCTTGC
    ATTGCTGGCA CTGGTCTGTC CACTCTTCGC CGCCTTGAGA ACCTCAGGCT GGACCAGCTA
    CATCAACTCT GGCCAGGAGG GCCCCGGGGC CTCAAACTGC CCAGCTTGAC CCATACGGGC
    ACCCCTGCGT CCCGCCGTCT GGGCCCTCCA GCTTCTCTTG ACCGCCGTAG CAGCAGCTCC
    AGCAGTGTCA GCTCGGCCTA TACTGTCAGC CGCCGCTCCT CCCTGGCCTC CCCTTTCCCG
    CCTGGCTCCC CACCAGAGAA TGGGGCATCC TCGCTGCCTG GCCTCACGCC TGCCCAGCAC
    TACCTGCTCC GGGCAAGATA TGCTTCAGCC AGGGGAGGTG GTACCCCACC CACTGCAGCA
    CCCAGCCTGG ATCGGATGGG GGGTCTTCCT GCACCTCCCT GGAGAAGCCG AGCTGAGTAT
    CCAGGTTACA ACCCCAACGT AGGAGTCACC CGGAGGGCCA GTGACCCAGC CCGGGCTGCT
    GACCGCCCTG CCCCGGCCAG AGTCCAGCGG TTCAAGAGCT TGGGTTGTGT CCACACACCC
    CCTACCATGA CAGGGGGAGG ACCGAACTTT GACCCCCAAC TCTCGACCTC TGTCTACTCA
    CCACAGCCCC CCAGCATCAC TGAGAATATT GCCATGGATA CCAGAGGGCT ACGGGAGGAG
    CCAGAGATTG GGACCTCCAT GATGGGCAGT GGTCTGAATC CCTATATGGA CTTCCCATCT
    ACTGATACTC TGGGATATGG GGGACCTGAG GGGGCAGCAG CTGAGCCTTA TGGAGCTAGG
    GGTCCAGGCT CTCTTCCTCT TGGACCTGGT CCACCCACCA ACTATGGCCC CAATCCCTGT
    CCCCAGCAGG TCTCCTATCC TGACCCCACT CCAGAAACAT GGGGTGAGTT CCCTTCCCAC
    ACCGGGCTGT ACCCAGGCCC CAAGCCTCCG GTTGGAGCCT ACAGCCAATG TCCTCGTCTT
    GAACATTATG GACAAGTGCA AGTCAAGCCA GAACAGGGGT GTCCAGTGGG TTCTGACTCC
    ACAGGACTGG CACCCTGCCT CAATGCCCAT CCCAATGAGG GGCCTCCACG ACCACAGCCT
    CTGTTCTCCC ACTACCCCCA GCCTCCCCCT CCCCAATATC CCCAGGCAGG CCCCTATCCC
    CAACCACCCC CTGATTACCT TCCTTCAGAA TCCAGGCCTG GCCTGGATTT TGATTCCCCC
    ACTCATTCCA CAGGACAGCT CAAGGCTCAG CTCGTGTGTG ATTATGTCCA GTCTCAACAG
    GAGCTGCTGT GGGAGGGTGG GGGGAGAGGC GATCCCCCAG TCCAGGAACC TCTCTACCAG
    AGTCCCAAGT TTCTGGGGGG TTCTCAGGTT AGCCCGAACC CTGCCAAGGC CCCAGTGGCC
    ACCTACGGAC CTGGCTTTGC ACCTAACTTG CCCCATCATA AGTCAGGCTC CTATCCCACC
    CCTTCACCAT GTCATGAAAA TTTTGCAGTG GGGGCAAACA AGTCTTCCCA TCGGGCAGCA
    GCACCACCCC GACTTCTGCC CCCACTGCCA ACTTGCTATG GGCCCCTCAA GGCAGGGGGA
    AGCAACCCCA GCTGTGGCCA TCCAGAGGTG GGCAGGTTGG GAGGGGGTCC TGCCTTATAC
    CCTCCTCCTG AAGGACAAGT GTGTAACCCT CTGGACTCTC TTGATCTTGA CAACACTCAG
    CTGGACTTTG TGGCTATTCT GGATGAGGCC CAGGGGCTGA GTCCTCCCCC TGCCCATGAT
    CAGGGGGAGA GCTCTGAACA CACCCCACCT CCGTCTGGGC CCCCCAACAT GGCTGTGGGT
    AACATGAGTG TGTTACTGGG GTCCCTGCCT GGGGAGACAC AATTCCTCAA CTCTAGTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66004
    Clone ID Related Accession (Same CDS sequence) NM_001163860.2 , XM_014740742.2 , XM_023642154.1 , XM_014740741.2 , XM_014740743.2 , XM_014740744.2
    Accession Version XM_014740742.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014740742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596228.2
    CDS508..3828
    Translation

    Target ORF information:

    RefSeq Version XM_014740742.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740742.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66004
    Clone ID Related Accession (Same CDS sequence) NM_001163860.2 , XM_014740742.2 , XM_023642154.1 , XM_014740741.2 , XM_014740743.2 , XM_014740744.2
    Accession Version XM_023642154.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023497922.1
    CDS487..3807
    Translation

    Target ORF information:

    RefSeq Version XM_023642154.1
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023642154.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66004
    Clone ID Related Accession (Same CDS sequence) NM_001163860.2 , XM_014740742.2 , XM_023642154.1 , XM_014740741.2 , XM_014740743.2 , XM_014740744.2
    Accession Version XM_014740741.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014740741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596227.2
    CDS589..3909
    Translation

    Target ORF information:

    RefSeq Version XM_014740741.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740741.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66004
    Clone ID Related Accession (Same CDS sequence) NM_001163860.2 , XM_014740742.2 , XM_023642154.1 , XM_014740741.2 , XM_014740743.2 , XM_014740744.2
    Accession Version XM_014740743.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014740743.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596229.2
    CDS568..3888
    Translation

    Target ORF information:

    RefSeq Version XM_014740743.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740743.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66004
    Clone ID Related Accession (Same CDS sequence) NM_001163860.2 , XM_014740742.2 , XM_023642154.1 , XM_014740741.2 , XM_014740743.2 , XM_014740744.2
    Accession Version XM_014740744.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014740744.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596230.2
    CDS443..3763
    Translation

    Target ORF information:

    RefSeq Version XM_014740744.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740744.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGGTACC ATCCCTTTCC ACAGTGGAGC CCAAGAGGGA GCGGGATGGA
    GGCCCCATCA GGGAAGAGAG CAGACTGGCC GTGCCGGAGG GGGCCATGAA GCCACAGCCA
    AGCCCTGGGG CCCAGTCATC CTGCAGCAGT GACCACTCCC CAGCAGGCAG CGCAGCCAAT
    ACAGACAGTG GTGTGGAAAT GACTGGAAAT GCAGGGGGCA GCACCGAGGA CCTGTCTAGC
    TTGGACGAGG GGCCTTGCAT TGCTGGCACT GGTCTGTCCA CTCTTCGCCG CCTTGAGAAC
    CTCAGGCTGG ACCAGCTACA TCAACTCCGG CCAGGAGGGC CCCGGGGCCT CAAACTGCCC
    AGCTTGACCC ATACGGGCAC CCCTGCGTCC CGCCGTCTGG GCCCTCCAGC TTCTCTTGAC
    CGCCGTAGCA GCAGCTCCAG CAGTGTCAGC TCGGCCTATA CTGTCAGCCG CCGCTCCTCC
    CTGGCCTCCC CTTTCCCGCC TGGCTCCCCA CCAGAGAATG GGGCATCCTC GCTGCCTGGC
    CTCACGCCTG CCCAGCACTA CCTGCTCCGG GCAAGATATG CTTCAGCCAG GGGAGGTGGT
    ACCCCACCCA CTGCAGCACC CAGCCTGGAT CGGATGGGGG GTCTTCCTGC ACCTCCCTGG
    AGAAGCCGAG CTGAGTATCC AGGTTACAAC CCCAACGTAG GAGTCACCCG GAGGGCCAGT
    GACCCAGCCC GGGCTGCTGA CCGCCCTGCC CCGGCCAGAG TCCAGCGGTT CAAGAGCTTG
    GGTTGTGTCC ACACACCCCC TACCATGACA GGGGGAGGAC CGAACTTTGA CCCCCAACTC
    TCGACCTCTG TCTACTCACC ACAGCCCCCC AGCATCACTG AGAATATTGC CATGGATACC
    AGAGGGCTAC GGGAGGAGCC AGAGATTGGG ACCTCCATGA TGGGCAGTGG TCTGAATCCC
    TATATGGACT TCCCATCTAC TGATACTCTG GGATATGGGG GACCTGAGGG GGCAGCAGCT
    GAGCCTTATG GAGCTAGGGG TCCAGGCTCT CTTCCTCTTG GACCTGGTCC ACCCACCAAC
    TATGGCCCCA ATCCCTGTCC CCAGCAGGTC TCCTATCCTG ACCCCACTCC AGAAACATGG
    GGTGAGTTCC CTTCCCACAC CGGGCTGTAC CCAGGCCCCA AGCCTCCGGT TGGAGCCTAC
    AGCCAATGTC CTCGTCTTGA ACATTATGGA CAAGTGCAAG TCAAGCCAGA ACAGGGGTGT
    CCAGTGGGTT CTGACTCCAC AGGACTGGCA CCCTGCCTCA ATGCCCATCC CAATGAGGGG
    CCTCCACGAC CACAGCCTCT GTTCTCCCAC TACCCCCAGC CTCCCCCTCC CCAATATCCC
    CAGGCAGGCC CCTATCCCCA ACCACCCCCT GATTACCTTC CTTCAGAATC CAGGCCTGGC
    CTGGATTTTG ATTCCCCCAC TCATTCCACA GGACAGCTCA AGGCTCAGCT CGTGTGTGAT
    TATGTCCAGT CTCAACAGGA GCTGCTGTGG GAGGGTGGGG GGAGAGGCGA TCCCCCAGTC
    CAGGAACCTC TCTACCAGAG TCCCAAGTTT CTGGGGGGTT CTCAGGTTAG CCCGAACCCT
    GCCAAGGCCC CAGTGGCCAC CTACGGACCT GGCTTTGCAC CTAACTTGCC CCATCATAAG
    TCAGGCTCCT ATCCCACCCC TTCACCATGT CATGAAAATT TTGCAGTGGG GGCAAACAAG
    TCTTCCCATC GGGCAGCAGC ACCACCCCGA CTTCTGCCCC CACTGCCAAC TTGCTATGGG
    CCCCTCAAGG CAGGGGGAAG CAACCCCAGC TGTGGCCATC CAGAGGTGGG CAGGTTGGGA
    GGGGGTCCTG CCTTATACCC TCCTCCTGAA GGACAAGTGT GTAACCCTCT GGACTCTCTT
    GATCTTGACA ACACTCAGCT GGACTTTGTG GCTATTCTGG ATGAGGCCCA GGGGCTGAGT
    CCTCCCCCTG CCCATGATCA GGGGGAGAGC TCTGAACACA CCCCACCTCC GTCTGGGCCC
    CCCAACATGG CTGTGGGTAA CATGAGTGTG TTACTGGGGT CCCTGCCTGG GGAGACACAA
    TTCCTCAACT CTAGTGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66005
    Clone ID Related Accession (Same CDS sequence) XM_014740745.2
    Accession Version XM_014740745.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014740745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGAAGCC ACAGCCAAGC CCTGGGGCCC AGTCATCCTG CAGCAGTGAC
    CACTCCCCAG CAGGCAGCGC AGCCAATACA GACAGTGGTG TGGAAATGAC TGGAAATGCA
    GGGGGCAGCA CCGAGGACCT GTCTAGCTTG GACGAGGGGC CTTGCATTGC TGGCACTGGT
    CTGTCCACTC TTCGCCGCCT TGAGAACCTC AGGCTGGACC AGCTACATCA ACTCCGGCCA
    GGAGGGCCCC GGGGCCTCAA ACTGCCCAGC TTGACCCATA CGGGCACCCC TGCGTCCCGC
    CGTCTGGGCC CTCCAGCTTC TCTTGACCGC CGTAGCAGCA GCTCCAGCAG TGTCAGCTCG
    GCCTATACTG TCAGCCGCCG CTCCTCCCTG GCCTCCCCTT TCCCGCCTGG CTCCCCACCA
    GAGAATGGGG CATCCTCGCT GCCTGGCCTC ACGCCTGCCC AGCACTACCT GCTCCGGGCA
    AGATATGCTT CAGCCAGGGG AGGTGGTACC CCACCCACTG CAGCACCCAG CCTGGATCGG
    ATGGGGGGTC TTCCTGCACC TCCCTGGAGA AGCCGAGCTG AGTATCCAGG TTACAACCCC
    AACGTAGGAG TCACCCGGAG GGCCAGTGAC CCAGCCCGGG CTGCTGACCG CCCTGCCCCG
    GCCAGAGTCC AGCGGTTCAA GAGCTTGGGT TGTGTCCACA CACCCCCTAC CATGACAGGG
    GGAGGACCGA ACTTTGACCC CCAACTCTCG ACCTCTGTCT ACTCACCACA GCCCCCCAGC
    ATCACTGAGA ATATTGCCAT GGATACCAGA GGGCTACGGG AGGAGCCAGA GATTGGGACC
    TCCATGATGG GCAGTGGTCT GAATCCCTAT ATGGACTTCC CATCTACTGA TACTCTGGGA
    TATGGGGGAC CTGAGGGGGC AGCAGCTGAG CCTTATGGAG CTAGGGGTCC AGGCTCTCTT
    CCTCTTGGAC CTGGTCCACC CACCAACTAT GGCCCCAATC CCTGTCCCCA GCAGGTCTCC
    TATCCTGACC CCACTCCAGA AACATGGGGT GAGTTCCCTT CCCACACCGG GCTGTACCCA
    GGCCCCAAGC CTCCGGTTGG AGCCTACAGC CAATGTCCTC GTCTTGAACA TTATGGACAA
    GTGCAAGTCA AGCCAGAACA GGGGTGTCCA GTGGGTTCTG ACTCCACAGG ACTGGCACCC
    TGCCTCAATG CCCATCCCAA TGAGGGGCCT CCACGACCAC AGCCTCTGTT CTCCCACTAC
    CCCCAGCCTC CCCCTCCCCA ATATCCCCAG GCAGGCCCCT ATCCCCAACC ACCCCCTGAT
    TACCTTCCTT CAGAATCCAG GCCTGGCCTG GATTTTGATT CCCCCACTCA TTCCACAGGA
    CAGCTCAAGG CTCAGCTCGT GTGTGATTAT GTCCAGTCTC AACAGGAGCT GCTGTGGGAG
    GGTGGGGGGA GAGGCGATCC CCCAGTCCAG GAACCTCTCT ACCAGAGTCC CAAGTTTCTG
    GGGGGTTCTC AGGTTAGCCC GAACCCTGCC AAGGCCCCAG TGGCCACCTA CGGACCTGGC
    TTTGCACCTA ACTTGCCCCA TCATAAGTCA GGCTCCTATC CCACCCCTTC ACCATGTCAT
    GAAAATTTTG CAGTGGGGGC AAACAAGTCT TCCCATCGGG CAGCAGCACC ACCCCGACTT
    CTGCCCCCAC TGCCAACTTG CTATGGGCCC CTCAAGGCAG GGGGAAGCAA CCCCAGCTGT
    GGCCATCCAG AGGTGGGCAG GTTGGGAGGG GGTCCTGCCT TATACCCTCC TCCTGAAGGA
    CAAGTGTGTA ACCCTCTGGA CTCTCTTGAT CTTGACAACA CTCAGCTGGA CTTTGTGGCT
    ATTCTGGATG AGGCCCAGGG GCTGAGTCCT CCCCCTGCCC ATGATCAGGG GGAGAGCTCT
    GAACACACCC CACCTCCGTC TGGGCCCCCC AACATGGCTG TGGGTAACAT GAGTGTGTTA
    CTGGGGTCCC TGCCTGGGGA GACACAATTC CTCAACTCTA GTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596231.2
    CDS590..3817
    Translation

    Target ORF information:

    RefSeq Version XM_014740745.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740745.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTCAACT CGATGACCCC ACCACCAGTC AGTAGCTATG GCGAACCCTG CTGTCTCCGG 
    CCCCTCCCCA GTCAGGGGGC CCCCAGCATG GGGACAGAAG GACTGTCTGG TCTGCCCTTC
    TGCCACCAGG CCAACCTCAT GTCCGGCCCC CACAGTTATG GGCCAGCCAG AGAGACCAAC
    AGCTGCACTG AGGCCCCACT TTTTCCTCCT CCCCGGAGTG CAGTCAAGTT GACCAAGAAG
    CGGGCACTCT CCATCTCACC CCTCTCGGAC GCCAGCCTGG ACCTGCAGAC AGTTATCCGC
    ACCTCGCCCA GCTCCCTTGT GGCCTTTATC AACTCACGCT GTGCATCTCC GGGAGGCTCC
    TATGGTCACC TCTCCATCAG CACCATGAGC CCATCTCTGG GATTCCCAGC CCAGATGAAT
    CACCAAAAAG GGACCTCGCC TTCCTTTGCG GTCCAGCCCT GTGGTCCCCA TGACCCCACC
    CACAGTGGGA TGATGCCGCA TCCTCAGTCC CGGGGACCCC TCCCAACTTG CCAGCTGAAG
    TCTGAGCTGG ACATGCTGGT TAGCAAATGC CCCGAGGAGC CCTTGGAAGG TGATATGTCC
    AGCCCCAACT CCACTGGCAC ACAGGATCCC CTGCTGGGGC TGCTGGATGG GCGGGAGGAT
    CTGGAGCGAG AAGAGAAGCC CGAGCCTGAG TCTGTGTATG AGACTGACTG CCGCTGGGAT
    GGCTGCAGCC AGGAGTTTGA CTCCCAGGAG CAGCTAGTGC ACCACATCAA CAGCGAGCAC
    ATCCACGGGG AGCGGAAGGA GTTCGTGTGC CATTGGGGGG GCTGCTCCCG GGAGCTGAGG
    CCCTTCAAAG CTCAGTACAT GCTGGTGGTC CACATGCGCA GACACACGGG CGAGAAGCCA
    CACAAGTGCA CGTTTGAGGG GTGCCGGAAG TCATACTCAC GCCTCGAAAA TCTGAAGACG
    CACCTGCGGT CCCACACGGG TGAGAAGCCG TACATGTGTG AGCATGAGGG CTGCAGCAAG
    GCCTTCAGCA ATGCCAGTGA CCGGGCCAAG CACCAGAACC GGACCCACTC CAATGAGAAG
    CCGTACGTGT GTAAGCTCCC CGGCTGTACT AAACGCTACA CAGATCCCAG CTCGCTCCGA
    AAACACGTCA AGACGGTCCA TGGTCCTGAT GCCCATGTGA CCAAGCGGCA CCGAGGCGAC
    GGCCCCCTAC CCCGGAAGCC ACAGCCAAGC CCTGGGGCCC AGTCATCCTG CAGCAGTGAC
    CACTCCCCAG CAGGCAGCGC AGCCAATACA GACAGTGGTG TGGAAATGAC TGGAAATGCA
    GGGGGCAGCA CCGAGGACCT GTCTAGCTTG GACGAGGGGC CTTGCATTGC TGGCACTGGT
    CTGTCCACTC TTCGCCGCCT TGAGAACCTC AGGCTGGACC AGCTACATCA ACTCCGGCCA
    GGAGGGCCCC GGGGCCTCAA ACTGCCCAGC TTGACCCATA CGGGCACCCC TGCGTCCCGC
    CGTCTGGGCC CTCCAGCTTC TCTTGACCGC CGTAGCAGCA GCTCCAGCAG TGTCAGCTCG
    GCCTATACTG TCAGCCGCCG CTCCTCCCTG GCCTCCCCTT TCCCGCCTGG CTCCCCACCA
    GAGAATGGGG CATCCTCGCT GCCTGGCCTC ACGCCTGCCC AGCACTACCT GCTCCGGGCA
    AGATATGCTT CAGCCAGGGG AGGTGGTACC CCACCCACTG CAGCACCCAG CCTGGATCGG
    ATGGGGGGTC TTCCTGCACC TCCCTGGAGA AGCCGAGCTG AGTATCCAGG TTACAACCCC
    AACGTAGGAG TCACCCGGAG GGCCAGTGAC CCAGCCCGGG CTGCTGACCG CCCTGCCCCG
    GCCAGAGTCC AGCGGTTCAA GAGCTTGGGT TGTGTCCACA CACCCCCTAC CATGACAGGG
    GGAGGACCGA ACTTTGACCC CCAACTCTCG ACCTCTGTCT ACTCACCACA GCCCCCCAGC
    ATCACTGAGA ATATTGCCAT GGATACCAGA GGGCTACGGG AGGAGCCAGA GATTGGGACC
    TCCATGATGG GCAGTGGTCT GAATCCCTAT ATGGACTTCC CATCTACTGA TACTCTGGGA
    TATGGGGGAC CTGAGGGGGC AGCAGCTGAG CCTTATGGAG CTAGGGGTCC AGGCTCTCTT
    CCTCTTGGAC CTGGTCCACC CACCAACTAT GGCCCCAATC CCTGTCCCCA GCAGGTCTCC
    TATCCTGACC CCACTCCAGA AACATGGGGT GAGTTCCCTT CCCACACCGG GCTGTACCCA
    GGCCCCAAGC CTCCGGTTGG AGCCTACAGC CAATGTCCTC GTCTTGAACA TTATGGACAA
    GTGCAAGTCA AGCCAGAACA GGGGTGTCCA GTGGGTTCTG ACTCCACAGG ACTGGCACCC
    TGCCTCAATG CCCATCCCAA TGAGGGGCCT CCACGACCAC AGCCTCTGTT CTCCCACTAC
    CCCCAGCCTC CCCCTCCCCA ATATCCCCAG GCAGGCCCCT ATCCCCAACC ACCCCCTGAT
    TACCTTCCTT CAGAATCCAG GCCTGGCCTG GATTTTGATT CCCCCACTCA TTCCACAGGA
    CAGCTCAAGG CTCAGCTCGT GTGTGATTAT GTCCAGTCTC AACAGGAGCT GCTGTGGGAG
    GGTGGGGGGA GAGGCGATCC CCCAGTCCAG GAACCTCTCT ACCAGAGTCC CAAGTTTCTG
    GGGGGTTCTC AGGTTAGCCC GAACCCTGCC AAGGCCCCAG TGGCCACCTA CGGACCTGGC
    TTTGCACCTA ACTTGCCCCA TCATAAGTCA GGCTCCTATC CCACCCCTTC ACCATGTCAT
    GAAAATTTTG CAGTGGGGGC AAACAAGTCT TCCCATCGGG CAGCAGCACC ACCCCGACTT
    CTGCCCCCAC TGCCAACTTG CTATGGGCCC CTCAAGGCAG GGGGAAGCAA CCCCAGCTGT
    GGCCATCCAG AGGTGGGCAG GTTGGGAGGG GGTCCTGCCT TATACCCTCC TCCTGAAGGA
    CAAGTGTGTA ACCCTCTGGA CTCTCTTGAT CTTGACAACA CTCAGCTGGA CTTTGTGGCT
    ATTCTGGATG AGGCCCAGGG GCTGAGTCCT CCCCCTGCCC ATGATCAGGG GGAGAGCTCT
    GAACACACCC CACCTCCGTC TGGGCCCCCC AACATGGCTG TGGGTAACAT GAGTGTGTTA
    CTGGGGTCCC TGCCTGGGGA GACACAATTC CTCAACTCTA GTGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb66006
    Clone ID Related Accession (Same CDS sequence) XM_014740746.2
    Accession Version XM_014740746.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product zinc finger protein GLI1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009149.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014740746.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGCC CCCACAGTTA TGGGCCAGCC AGAGAGACCA ACAGCTGCAC TGAGGCCCCA 
    CTTTTTCCTC CTCCCCGGAG TGCAGTCAAG TTGACCAAGA AGCGGGCACT CTCCATCTCA
    CCCCTCTCGG ACGCCAGCCT GGACCTGCAG ACAGTTATCC GCACCTCGCC CAGCTCCCTT
    GTGGCCTTTA TCAACTCACG CTGTGCATCT CCGGGAGGCT CCTATGGTCA CCTCTCCATC
    AGCACCATGA GCCCATCTCT GGGATTCCCA GCCCAGATGA ATCACCAAAA AGGGACCTCG
    CCTTCCTTTG CGGTCCAGCC CTGTGGTCCC CATGACCCCA CCCACAGTGG GATGATGCCG
    CATCCTCAGT CCCGGGGACC CCTCCCAACT TGCCAGCTGA AGTCTGAGCT GGACATGCTG
    GTTAGCAAAT GCCCCGAGGA GCCCTTGGAA GGTGATATGT CCAGCCCCAA CTCCACTGGC
    ACACAGGATC CCCTGCTGGG GCTGCTGGAT GGGCGGGAGG ATCTGGAGCG AGAAGAGAAG
    CCCGAGCCTG AGTCTGTGTA TGAGACTGAC TGCCGCTGGG ATGGCTGCAG CCAGGAGTTT
    GACTCCCAGG AGCAGCTAGT GCACCACATC AACAGCGAGC ACATCCACGG GGAGCGGAAG
    GAGTTCGTGT GCCATTGGGG GGGCTGCTCC CGGGAGCTGA GGCCCTTCAA AGCTCAGTAC
    ATGCTGGTGG TCCACATGCG CAGACACACG GGCGAGAAGC CACACAAGTG CACGTTTGAG
    GGGTGCCGGA AGTCATACTC ACGCCTCGAA AATCTGAAGA CGCACCTGCG GTCCCACACG
    GGTGAGAAGC CGTACATGTG TGAGCATGAG GGCTGCAGCA AGGCCTTCAG CAATGCCAGT
    GACCGGGCCA AGCACCAGAA CCGGACCCAC TCCAATGAGA AGCCGTACGT GTGTAAGCTC
    CCCGGCTGTA CTAAACGCTA CACAGATCCC AGCTCGCTCC GAAAACACGT CAAGACGGTC
    CATGGTCCTG ATGCCCATGT GACCAAGCGG CACCGAGGCG ACGGCCCCCT ACCCCGGGTA
    CCATCCCTTT CCACAGTGGA GCCCAAGAGG GAGCGGGATG GAGGCCCCAT CAGGGAAGAG
    AGCAGACTGG CCGTGCCGGA GGGGGCCATG AAGCCACAGC CAAGCCCTGG GGCCCAGTCA
    TCCTGCAGCA GTGACCACTC CCCAGCAGGC AGCGCAGCCA ATACAGACAG TGGTGTGGAA
    ATGACTGGAA ATGCAGGGGG CAGCACCGAG GACCTGTCTA GCTTGGACGA GGGGCCTTGC
    ATTGCTGGCA CTGGTCTGTC CACTCTTCGC CGCCTTGAGA ACCTCAGGCT GGACCAGCTA
    CATCAACTCC GGCCAGGAGG GCCCCGGGGC CTCAAACTGC CCAGCTTGAC CCATACGGGC
    ACCCCTGCGT CCCGCCGTCT GGGCCCTCCA GCTTCTCTTG ACCGCCGTAG CAGCAGCTCC
    AGCAGTGTCA GCTCGGCCTA TACTGTCAGC CGCCGCTCCT CCCTGGCCTC CCCTTTCCCG
    CCTGGCTCCC CACCAGAGAA TGGGGCATCC TCGCTGCCTG GCCTCACGCC TGCCCAGCAC
    TACCTGCTCC GGGCAAGATA TGCTTCAGCC AGGGGAGGTG GTACCCCACC CACTGCAGCA
    CCCAGCCTGG ATCGGATGGG GGGTCTTCCT GCACCTCCCT GGAGAAGCCG AGCTGAGTAT
    CCAGGTTACA ACCCCAACGT AGGAGTCACC CGGAGGGCCA GTGACCCAGC CCGGGCTGCT
    GACCGCCCTG CCCCGGCCAG AGTCCAGCGG TTCAAGAGCT TGGGTTGTGT CCACACACCC
    CCTACCATGA CAGGGGGAGG ACCGAACTTT GACCCCCAAC TCTCGACCTC TGTCTACTCA
    CCACAGCCCC CCAGCATCAC TGAGAATATT GCCATGGATA CCAGAGGGCT ACGGGAGGAG
    CCAGAGATTG GGACCTCCAT GATGGGCAGT GGTCTGAATC CCTATATGGA CTTCCCATCT
    ACTGATACTC TGGGATATGG GGGACCTGAG GGGGCAGCAG CTGAGCCTTA TGGAGCTAGG
    GGTCCAGGCT CTCTTCCTCT TGGACCTGGT CCACCCACCA ACTATGGCCC CAATCCCTGT
    CCCCAGCAGG TCTCCTATCC TGACCCCACT CCAGAAACAT GGGGTGAGTT CCCTTCCCAC
    ACCGGGCTGT ACCCAGGCCC CAAGCCTCCG GTTGGAGCCT ACAGCCAATG TCCTCGTCTT
    GAACATTATG GACAAGTGCA AGTCAAGCCA GAACAGGGGT GTCCAGTGGG TTCTGACTCC
    ACAGGACTGG CACCCTGCCT CAATGCCCAT CCCAATGAGG GGCCTCCACG ACCACAGCCT
    CTGTTCTCCC ACTACCCCCA GCCTCCCCCT CCCCAATATC CCCAGGCAGG CCCCTATCCC
    CAACCACCCC CTGATTACCT TCCTTCAGAA TCCAGGCCTG GCCTGGATTT TGATTCCCCC
    ACTCATTCCA CAGGACAGCT CAAGGCTCAG CTCGTGTGTG ATTATGTCCA GTCTCAACAG
    GAGCTGCTGT GGGAGGGTGG GGGGAGAGGC GATCCCCCAG TCCAGGAACC TCTCTACCAG
    AGTCCCAAGT TTCTGGGGGG TTCTCAGGTT AGCCCGAACC CTGCCAAGGC CCCAGTGGCC
    ACCTACGGAC CTGGCTTTGC ACCTAACTTG CCCCATCATA AGTCAGGCTC CTATCCCACC
    CCTTCACCAT GTCATGAAAA TTTTGCAGTG GGGGCAAACA AGTCTTCCCA TCGGGCAGCA
    GCACCACCCC GACTTCTGCC CCCACTGCCA ACTTGCTATG GGCCCCTCAA GGCAGGGGGA
    AGCAACCCCA GCTGTGGCCA TCCAGAGGTG GGCAGGTTGG GAGGGGGTCC TGCCTTATAC
    CCTCCTCCTG AAGGACAAGT GTGTAACCCT CTGGACTCTC TTGATCTTGA CAACACTCAG
    CTGGACTTTG TGGCTATTCT GGATGAGGCC CAGGGGCTGA GTCCTCCCCC TGCCCATGAT
    CAGGGGGAGA GCTCTGAACA CACCCCACCT CCGTCTGGGC CCCCCAACAT GGCTGTGGGT
    AACATGAGTG TGTTACTGGG GTCCCTGCCT GGGGAGACAC AATTCCTCAA CTCTAGTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014596232.2
    CDS579..3761
    Translation

    Target ORF information:

    RefSeq Version XM_014740746.2
    Organism Equus caballus(horse)
    Definition Equus caballus GLI family zinc finger 1 (GLI1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014740746.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCCGGCC CCCACAGTTA TGGGCCAGCC AGAGAGACCA ACAGCTGCAC TGAGGCCCCA 
    CTTTTTCCTC CTCCCCGGAG TGCAGTCAAG TTGACCAAGA AGCGGGCACT CTCCATCTCA
    CCCCTCTCGG ACGCCAGCCT GGACCTGCAG ACAGTTATCC GCACCTCGCC CAGCTCCCTT
    GTGGCCTTTA TCAACTCACG CTGTGCATCT CCGGGAGGCT CCTATGGTCA CCTCTCCATC
    AGCACCATGA GCCCATCTCT GGGATTCCCA GCCCAGATGA ATCACCAAAA AGGGACCTCG
    CCTTCCTTTG CGGTCCAGCC CTGTGGTCCC CATGACCCCA CCCACAGTGG GATGATGCCG
    CATCCTCAGT CCCGGGGACC CCTCCCAACT TGCCAGCTGA AGTCTGAGCT GGACATGCTG
    GTTAGCAAAT GCCCCGAGGA GCCCTTGGAA GGTGATATGT CCAGCCCCAA CTCCACTGGC
    ACACAGGATC CCCTGCTGGG GCTGCTGGAT GGGCGGGAGG ATCTGGAGCG AGAAGAGAAG
    CCCGAGCCTG AGTCTGTGTA TGAGACTGAC TGCCGCTGGG ATGGCTGCAG CCAGGAGTTT
    GACTCCCAGG AGCAGCTAGT GCACCACATC AACAGCGAGC ACATCCACGG GGAGCGGAAG
    GAGTTCGTGT GCCATTGGGG GGGCTGCTCC CGGGAGCTGA GGCCCTTCAA AGCTCAGTAC
    ATGCTGGTGG TCCACATGCG CAGACACACG GGCGAGAAGC CACACAAGTG CACGTTTGAG
    GGGTGCCGGA AGTCATACTC ACGCCTCGAA AATCTGAAGA CGCACCTGCG GTCCCACACG
    GGTGAGAAGC CGTACATGTG TGAGCATGAG GGCTGCAGCA AGGCCTTCAG CAATGCCAGT
    GACCGGGCCA AGCACCAGAA CCGGACCCAC TCCAATGAGA AGCCGTACGT GTGTAAGCTC
    CCCGGCTGTA CTAAACGCTA CACAGATCCC AGCTCGCTCC GAAAACACGT CAAGACGGTC
    CATGGTCCTG ATGCCCATGT GACCAAGCGG CACCGAGGCG ACGGCCCCCT ACCCCGGGTA
    CCATCCCTTT CCACAGTGGA GCCCAAGAGG GAGCGGGATG GAGGCCCCAT CAGGGAAGAG
    AGCAGACTGG CCGTGCCGGA GGGGGCCATG AAGCCACAGC CAAGCCCTGG GGCCCAGTCA
    TCCTGCAGCA GTGACCACTC CCCAGCAGGC AGCGCAGCCA ATACAGACAG TGGTGTGGAA
    ATGACTGGAA ATGCAGGGGG CAGCACCGAG GACCTGTCTA GCTTGGACGA GGGGCCTTGC
    ATTGCTGGCA CTGGTCTGTC CACTCTTCGC CGCCTTGAGA ACCTCAGGCT GGACCAGCTA
    CATCAACTCC GGCCAGGAGG GCCCCGGGGC CTCAAACTGC CCAGCTTGAC CCATACGGGC
    ACCCCTGCGT CCCGCCGTCT GGGCCCTCCA GCTTCTCTTG ACCGCCGTAG CAGCAGCTCC
    AGCAGTGTCA GCTCGGCCTA TACTGTCAGC CGCCGCTCCT CCCTGGCCTC CCCTTTCCCG
    CCTGGCTCCC CACCAGAGAA TGGGGCATCC TCGCTGCCTG GCCTCACGCC TGCCCAGCAC
    TACCTGCTCC GGGCAAGATA TGCTTCAGCC AGGGGAGGTG GTACCCCACC CACTGCAGCA
    CCCAGCCTGG ATCGGATGGG GGGTCTTCCT GCACCTCCCT GGAGAAGCCG AGCTGAGTAT
    CCAGGTTACA ACCCCAACGT AGGAGTCACC CGGAGGGCCA GTGACCCAGC CCGGGCTGCT
    GACCGCCCTG CCCCGGCCAG AGTCCAGCGG TTCAAGAGCT TGGGTTGTGT CCACACACCC
    CCTACCATGA CAGGGGGAGG ACCGAACTTT GACCCCCAAC TCTCGACCTC TGTCTACTCA
    CCACAGCCCC CCAGCATCAC TGAGAATATT GCCATGGATA CCAGAGGGCT ACGGGAGGAG
    CCAGAGATTG GGACCTCCAT GATGGGCAGT GGTCTGAATC CCTATATGGA CTTCCCATCT
    ACTGATACTC TGGGATATGG GGGACCTGAG GGGGCAGCAG CTGAGCCTTA TGGAGCTAGG
    GGTCCAGGCT CTCTTCCTCT TGGACCTGGT CCACCCACCA ACTATGGCCC CAATCCCTGT
    CCCCAGCAGG TCTCCTATCC TGACCCCACT CCAGAAACAT GGGGTGAGTT CCCTTCCCAC
    ACCGGGCTGT ACCCAGGCCC CAAGCCTCCG GTTGGAGCCT ACAGCCAATG TCCTCGTCTT
    GAACATTATG GACAAGTGCA AGTCAAGCCA GAACAGGGGT GTCCAGTGGG TTCTGACTCC
    ACAGGACTGG CACCCTGCCT CAATGCCCAT CCCAATGAGG GGCCTCCACG ACCACAGCCT
    CTGTTCTCCC ACTACCCCCA GCCTCCCCCT CCCCAATATC CCCAGGCAGG CCCCTATCCC
    CAACCACCCC CTGATTACCT TCCTTCAGAA TCCAGGCCTG GCCTGGATTT TGATTCCCCC
    ACTCATTCCA CAGGACAGCT CAAGGCTCAG CTCGTGTGTG ATTATGTCCA GTCTCAACAG
    GAGCTGCTGT GGGAGGGTGG GGGGAGAGGC GATCCCCCAG TCCAGGAACC TCTCTACCAG
    AGTCCCAAGT TTCTGGGGGG TTCTCAGGTT AGCCCGAACC CTGCCAAGGC CCCAGTGGCC
    ACCTACGGAC CTGGCTTTGC ACCTAACTTG CCCCATCATA AGTCAGGCTC CTATCCCACC
    CCTTCACCAT GTCATGAAAA TTTTGCAGTG GGGGCAAACA AGTCTTCCCA TCGGGCAGCA
    GCACCACCCC GACTTCTGCC CCCACTGCCA ACTTGCTATG GGCCCCTCAA GGCAGGGGGA
    AGCAACCCCA GCTGTGGCCA TCCAGAGGTG GGCAGGTTGG GAGGGGGTCC TGCCTTATAC
    CCTCCTCCTG AAGGACAAGT GTGTAACCCT CTGGACTCTC TTGATCTTGA CAACACTCAG
    CTGGACTTTG TGGCTATTCT GGATGAGGCC CAGGGGCTGA GTCCTCCCCC TGCCCATGAT
    CAGGGGGAGA GCTCTGAACA CACCCCACCT CCGTCTGGGC CCCCCAACAT GGCTGTGGGT
    AACATGAGTG TGTTACTGGG GTCCCTGCCT GGGGAGACAC AATTCCTCAA CTCTAGTGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.