DDX27 cDNA ORF clone, Elephantulus edwardii(Cape elephant shrew)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX27
Entrez Gene ID	102863160
Full Name	DEAD-box helicase 27
Gene Type	protein-coding
Organism	Elephantulus edwardii(Cape elephant shrew)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006881622.1	XP_006881684.1	probable ATP-dependent RNA helicase DDX27

Pathways from BioSystems
Gene Ontology

The following DDX27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEl37929	XM_006881622.1 Latest version!	Elephantulus edwardii DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX27 ( XM_006881622.1 ) cDNA ORF clone, Elephantulus edwardii(Cape elephant shrew) -> XP_006881684.1 Elephantulus edwardii DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), mRNA.

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CloneID	OEl37929
Clone ID Related Accession (Same CDS sequence)	XM_006881622.1
Accession Version	XM_006881622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-02-17
Organism	Elephantulus edwardii(Cape elephant shrew)
Product	probable ATP-dependent RNA helicase DDX27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006399765.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Elephantulus edwardii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAG AGCTGGGTTT GATCGGGACC ATAGGCGAGG ATGATGATGT GCCGCTGGAG 
CCAGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCA TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG AAGTGCCGAT TTCAACCCCG ACTTTGTCTT TACAGAGAAG 
GAGGGGTTGT ATGATAGCAG CTGGGCGATG GCTGACGTCC TGAGCCAGCT CAAGAAGAAG 
AGAGCAGCTA CTACATTGGA TGAGAAGATT GAGAAAGTTC GAAAGAAAAG GAAGATGGAG 
ACAAAAGAGG CCAAATCAGG GACGCTGGAG AAGGAGAAAG AAGCAGAGGC TGGAAACTCA 
GAGAAGGAGC GAGACGTAGC GGGGAGCTCT GACGGCGAGG AGCCGGAAGC CCTTGACGGG 
AAAGACAAGG AGGGCTCAGA GGACGAAGAC TCGGAGACGG ACTATTCCTC CGCCGAAGAG 
AACATCCTCA CCAAAGCAGA TACCCTCAAA GTAAAGGAGA AGAAAAAGAA GAAGAAAGGG 
CAGGAAGCAG GAGGATTTTT CGAAGATGCA TCTCAGTACG ATGAGAGCCT CTCGTTCCAG 
GACATGAACC TTTCCCGGCC TCTGCTGAAG GCCATCACAA CCATGGGCTT CAAGCAGCCC 
ACCCCTATCC AGAAGGCATG TATACCTGTG GGTCTGCTGG GGAAGGACAT CTGTGCTTGT 
GCAGCCACCG GGACAGGTAA AACCGCTGCT TTCGCCCTGC CTGTCCTGGA GCGTCTGATC 
TACAAGCCCC GCCAGGCGGC GGTCACCCGC GTGCTCGTGC TGGTGCCCAC TCGAGAGCTG 
GGCATCCAGG TGCACTCCGT CACCAAGCAG TTGGCCCAGT TCTGCAACAT CACCACCTGC 
CTCGCTGTGG GTGGCCTGGA TGTGAAGTCT CAGGAAGCAG CTCTCCGGGC CGCGCCCGAC 
ATCCTCATCG CCACCCCGGG TCGGCTCATC GACCACCTCC ACAACTGCCC TTCCTTCCAC 
CTGAGCAGCA TCGAGGTGCT CATCCTGGAT GAGGCCGACA GGATGCTGGA CGAGTACTTT 
GAGGAGCAGA TGAAGGAGAT CATCCGCATG TGTTCCCACC ACCGCCAGAC CATGCTTTTC 
TCGGCCACGA TGACAGACGA GGTCAAAGAT CTGGCTTCTG TCTCCTTGAA GAACCCTGTC 
CGCATATTTG TGAACAGCAA CACCGACGTG GCTCCTTTCC TGCGGCAGGA ATTCATCCGC 
ATCCGTCCCA ACCGGGAAGG GGACCGAGAG GCCATCGTGG CAGCTCTGTT GACGAGAACC 
TTCACCGACC ACGTGATGCT GTTCACACAG ACCAAGAAGC AGGCGCACCG CATGCACATC 
CTGCTGGGAC TCCTGGGCCT GCAGGTGGGC GAGCTCCATG GCAACCTGTC CCAGACCCAG 
CGGCTGGAAG CCCTGAGGCG CTTTAAGGAT GAACAGATTG ACATTCTGGT GGCCACAGAT 
GTGGCAGCCC GTGGACTTGA CATCGAGGGT GTCAAAACGG TCATCAACTT CACCATGCCC 
AACACCATCA AGCACTATGT CCACCGGGTG GGGCGGACGG CACGTGCTGG CCGGGCTGGG 
CGTTCTGTGT CTCTGGTGGG AGAAGAGGAG CGGAAGATGC TGAAAGAGAT TGTGAAGTCG 
GCCAAGGCCC CCGTGAAGGC CCGCATACTG CCTCAAGACG TCATCCTCAA GTTCCGAGAC 
AAGATTGAGA AGATGGAGAA GGACGTGTAT GCCGTCCTGC AGCTGGAGGC TGAGGAGAAG 
GAGATGCAGC ACTCGGAGGC CCAGATCAAC ACAGCAAAGC GGCTTCTGGA GAAAGGGAAG 
GAGGCTTCGA GCCAAGAGCC CGAGAGGAGC TGGTTCCAGA CCAAAGAGGA GAGGAAGAAG 
GAGAAAATTG CCAAGGCTCT GCAGGAGTTT GACTTAGCCT TAAGAGGAAA GAAGAAAAGG 
AAGAAGTTTA TGAACGATGC CAAGAAGAAG GGGGAGATGA CGGCAGAAGA GAGGTCCCAG 
TTTGAAATCC TCAAGGCACA GATGTTCGCT GAGCGCCTGG CCAAGAGGAA CCGCAGGGCC 
AAGCGGGCCC GCGCGATGCC CGAGGAGGAG CCGGCCAGAG GCCCTGCCAA GAAGCAGAAG 
CAGGTGAAGA AATCTGTATT TGACGAAGAG CTGACGAACA CTAGCAAGAA GGCCCTGAAG 
CAGTATCGGG CTGGCCCATC CTTTGAAGAA AAGAAAAAGC TGGGCTTGCA GCACCAGAGG 
CGAGGAGGAA ACTTTAAATC TAAATCCAGG TATAAGCGAA GGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006881684.1
CDS	31..2358
Translation	MFEELGLIGTIGEDDDVPLEPESDSGDEEEEGPIVLGRKQKALQKNRSADFNPDFVFTEKEGLYDSSWAMADVLSQLKKKRAATTLDEKIEKVRKKRKMETKEAKSGTLEKEKEAEAGNSEKERDVAGSSDGEEPEALDGKDKEGSEDEDSETDYSSAEENILTKADTLKVKEKKKKKKGQEAGGFFEDASQYDESLSFQDMNLSRPLLKAITTMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKSAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQHSEAQINTAKRLLEKGKEASSQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMNDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRAKRARAMPEEEPARGPAKKQKQVKKSVFDEELTNTSKKALKQYRAGPSFEEKKKLGLQHQRRGGNFKSKSRYKRRK

Target ORF information:

RefSeq Version	XM_006881622.1
Organism	Elephantulus edwardii(Cape elephant shrew)
Definition	Elephantulus edwardii DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006881622.1

ORF Insert Sequence:

ATGTTTGAAG AGCTGGGTTT GATCGGGACC ATAGGCGAGG ATGATGATGT GCCGCTGGAG 
CCAGAGTCTG ACTCCGGGGA CGAGGAGGAG GAGGGGCCCA TTGTGCTGGG CAGAAAGCAG 
AAAGCCTTGC AGAAGAACCG AAGTGCCGAT TTCAACCCCG ACTTTGTCTT TACAGAGAAG 
GAGGGGTTGT ATGATAGCAG CTGGGCGATG GCTGACGTCC TGAGCCAGCT CAAGAAGAAG 
AGAGCAGCTA CTACATTGGA TGAGAAGATT GAGAAAGTTC GAAAGAAAAG GAAGATGGAG 
ACAAAAGAGG CCAAATCAGG GACGCTGGAG AAGGAGAAAG AAGCAGAGGC TGGAAACTCA 
GAGAAGGAGC GAGACGTAGC GGGGAGCTCT GACGGCGAGG AGCCGGAAGC CCTTGACGGG 
AAAGACAAGG AGGGCTCAGA GGACGAAGAC TCGGAGACGG ACTATTCCTC CGCCGAAGAG 
AACATCCTCA CCAAAGCAGA TACCCTCAAA GTAAAGGAGA AGAAAAAGAA GAAGAAAGGG 
CAGGAAGCAG GAGGATTTTT CGAAGATGCA TCTCAGTACG ATGAGAGCCT CTCGTTCCAG 
GACATGAACC TTTCCCGGCC TCTGCTGAAG GCCATCACAA CCATGGGCTT CAAGCAGCCC 
ACCCCTATCC AGAAGGCATG TATACCTGTG GGTCTGCTGG GGAAGGACAT CTGTGCTTGT 
GCAGCCACCG GGACAGGTAA AACCGCTGCT TTCGCCCTGC CTGTCCTGGA GCGTCTGATC 
TACAAGCCCC GCCAGGCGGC GGTCACCCGC GTGCTCGTGC TGGTGCCCAC TCGAGAGCTG 
GGCATCCAGG TGCACTCCGT CACCAAGCAG TTGGCCCAGT TCTGCAACAT CACCACCTGC 
CTCGCTGTGG GTGGCCTGGA TGTGAAGTCT CAGGAAGCAG CTCTCCGGGC CGCGCCCGAC 
ATCCTCATCG CCACCCCGGG TCGGCTCATC GACCACCTCC ACAACTGCCC TTCCTTCCAC 
CTGAGCAGCA TCGAGGTGCT CATCCTGGAT GAGGCCGACA GGATGCTGGA CGAGTACTTT 
GAGGAGCAGA TGAAGGAGAT CATCCGCATG TGTTCCCACC ACCGCCAGAC CATGCTTTTC 
TCGGCCACGA TGACAGACGA GGTCAAAGAT CTGGCTTCTG TCTCCTTGAA GAACCCTGTC 
CGCATATTTG TGAACAGCAA CACCGACGTG GCTCCTTTCC TGCGGCAGGA ATTCATCCGC 
ATCCGTCCCA ACCGGGAAGG GGACCGAGAG GCCATCGTGG CAGCTCTGTT GACGAGAACC 
TTCACCGACC ACGTGATGCT GTTCACACAG ACCAAGAAGC AGGCGCACCG CATGCACATC 
CTGCTGGGAC TCCTGGGCCT GCAGGTGGGC GAGCTCCATG GCAACCTGTC CCAGACCCAG 
CGGCTGGAAG CCCTGAGGCG CTTTAAGGAT GAACAGATTG ACATTCTGGT GGCCACAGAT 
GTGGCAGCCC GTGGACTTGA CATCGAGGGT GTCAAAACGG TCATCAACTT CACCATGCCC 
AACACCATCA AGCACTATGT CCACCGGGTG GGGCGGACGG CACGTGCTGG CCGGGCTGGG 
CGTTCTGTGT CTCTGGTGGG AGAAGAGGAG CGGAAGATGC TGAAAGAGAT TGTGAAGTCG 
GCCAAGGCCC CCGTGAAGGC CCGCATACTG CCTCAAGACG TCATCCTCAA GTTCCGAGAC 
AAGATTGAGA AGATGGAGAA GGACGTGTAT GCCGTCCTGC AGCTGGAGGC TGAGGAGAAG 
GAGATGCAGC ACTCGGAGGC CCAGATCAAC ACAGCAAAGC GGCTTCTGGA GAAAGGGAAG 
GAGGCTTCGA GCCAAGAGCC CGAGAGGAGC TGGTTCCAGA CCAAAGAGGA GAGGAAGAAG 
GAGAAAATTG CCAAGGCTCT GCAGGAGTTT GACTTAGCCT TAAGAGGAAA GAAGAAAAGG 
AAGAAGTTTA TGAACGATGC CAAGAAGAAG GGGGAGATGA CGGCAGAAGA GAGGTCCCAG 
TTTGAAATCC TCAAGGCACA GATGTTCGCT GAGCGCCTGG CCAAGAGGAA CCGCAGGGCC 
AAGCGGGCCC GCGCGATGCC CGAGGAGGAG CCGGCCAGAG GCCCTGCCAA GAAGCAGAAG 
CAGGTGAAGA AATCTGTATT TGACGAAGAG CTGACGAACA CTAGCAAGAA GGCCCTGAAG 
CAGTATCGGG CTGGCCCATC CTTTGAAGAA AAGAAAAAGC TGGGCTTGCA GCACCAGAGG 
CGAGGAGGAA ACTTTAAATC TAAATCCAGG TATAAGCGAA GGAAGTAG