Zwilch cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zwilch
Entrez Gene ID	59247
Full Name	CG18729 gene product from transcript CG18729-RA
Synonyms	CG18639,CG18729,Dmel\CG18729,zwilch
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100B6-100B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170542.2	NP_733421.1	zwilch

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	zwilch	NP_733421.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008706	XP_314811.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zwilch localizes to kinetochores and kinetochore microtubules in a manner identical to ZW10 and ROD.
Title: Zwilch, a new component of the ZW10/ROD complex required for kinetochore functions.

The following Zwilch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zwilch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08776	NM_170542.2 Latest version!	Drosophila melanogaster zwilch (Zwilch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zwilch ( NM_170542.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733421.1 Drosophila melanogaster zwilch (Zwilch), mRNA.

Price & Availability↑

CloneID	OFa08776
Clone ID Related Accession (Same CDS sequence)	NM_170542.2
Accession Version	NM_170542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	zwilch
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170542.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGCTT CTGCGAACTT GGCAAACGTT TACGCGGAGC TAATGCGGCG GTGTGGCGAG 
TCATACACGA TCACCTATGG AGCGCCACCC ACCTACTTGG TGAGCATGGT GGGGGCTGCG 
GAAGCTGGCA AGAAGATTGT GCTGGTCTTT AAGGAGGATC GCAATGGAGC AGTGGCGAGA 
CTCCGGACCA CGCCCACCAG GGCAGCCCCA AAAAAAGAGG GCTCTGCGGA CTTGGATCTG 
ACGGGAAGTC CTTTGAAGGA CGACTGCCTG GTGGACGCCA TAGCCGACTT GTCCATCGAC 
CTGCAGCTGG ATCACTCGAA TCCCTGGAAG CTGGAGGAGG AATATCAACG CGGAATACCC 
GTGGACAAGG CTAGGTCCAT CGTGTGCTCC GAGTTCCTGC AGCTGGCCGA GGGACTCGGA 
TCCGTGTGGT TTTTGTGCGA CGGCAGTGAT CTCGGGCAGA CTCAGTTGCT CCAGTACGAG 
TTCAACCCTA CGCACTTCTC CAGGGGAATA CTTAGCTACC AGGGTGTGCG CCCTGCTTAC 
TTAGTAACCT CGCAAGCCCT GGTGCGCCAT CATGGCAAGA CACCGGATGA AACTCTCATT 
GAAAATAGCT ACCAGGTCAA TCCCCACATG AGACTACGCT GCTCCTGGAC CTCTAGTGCT 
GCACTTCCGC TCCTGGTGAA CCTGAACGAC TGCGATGTGG CTTTGAATCA CACTTTTCGC 
GTGGGCGACT GCGGTCCTTT GACGCAGGAC TTTATGAATC AGCTGCGCAT TCTGGTCTAC 
ATACGCGAGG ACATCGTCTC CTACCACACG GATGTCAAGC AGGGCGTCTC TCGGGATCCC 
ACCTATCGTT GTGGCAGTGG CATTGACATG GACGAGCTGC GCGAGTCCAT TAATCAGACA 
ATGACAGATG TCTCTGGCCT TATAGGTCGG TATAGCATCA GCAATGCGGA GTTTGACATC 
GAAGATGTGG TGCAGAGGGC CAAGGTTCGC CAGCTCACGG ATCTAACCGA TAAGCTTTGG 
GAGCTGCTCA AGTGCTGCCA CTCCTACAAG GATCTCAAAA TTGCCTTTAG TATGCTCTTC 
CAATGTGCTG CACGGTGTAA CATAGTAAAC ACGCCTACTA ACAAAAATCG ACTTGCCAAG 
ATTATCACCG AGTTGGCCAA CCGTCGTCTG GCCATGCCTT GTTTAAGTGG GGCCGAGCCT 
CTGGAACTGC TTTTGGAAAT TGGCCTGGAG AAGTTGTACA AGGACTACGA GTTTATCTAC 
ACCGAGAGCA AGATGTGCAG CACCAATCTG TTGAAGGATG ACTCCAGCGG AGCGTCGATG 
GACGATGGTT CTCCCCAGAA TCTGCCCCAG CTTCGAAAAT CCCTGCACAA TGCGGTCAGG 
GGTGATCCGA CCCCTGGAGC TGGAATGCGA AAGACGCTGC TGCATCATCA CGGCGCTGTC 
AATTCGCGGA GTACAAAATA TGCCGGTAGC GACGATGATG CTGGGTTCAA AAACAGCCAT 
TTCGACGAGC ACGAGAGTAC GGAGAGAATC TCAAAGTTGT TTCAGATTCA CTGCACCTTA 
GAGCATCTCC TGATGATGCA CATCCACTTA AATCTGGCAA ATGTTTACAA CGATGTCTGT 
TCTGAATTGC TGAAGAAACC GCCTAAATTA GTAGAATCCA TCGATGATCA GCTAAGTGAT 
GTTATGGACA TTCGCCTGTC TGCCCACTAT GTGAGGGATC ACTTGGATGG CAAGGATCCC 
TACTCCCGGC ACATTACCAT GCGTTCGCAC AACAAGTTCC GCGAACTAAA GACCACCTTC 
TATTTCAATT CGGAAAACAT TTGTCCGCCG AACTTGGCTC AGTGTTTCCA GTGTGATGAC 
AAGGAGATGG TCAAGGAGCG CACATATCAT TCCTGGATAT ATCACAAGAT TCGCTCACTT 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733421.1
CDS	88..2013
Translation	MSASANLANVYAELMRRCGESYTITYGAPPTYLVSMVGAAEAGKKIVLVFKEDRNGAVARLRTTPTRAAPKKEGSADLDLTGSPLKDDCLVDAIADLSIDLQLDHSNPWKLEEEYQRGIPVDKARSIVCSEFLQLAEGLGSVWFLCDGSDLGQTQLLQYEFNPTHFSRGILSYQGVRPAYLVTSQALVRHHGKTPDETLIENSYQVNPHMRLRCSWTSSAALPLLVNLNDCDVALNHTFRVGDCGPLTQDFMNQLRILVYIREDIVSYHTDVKQGVSRDPTYRCGSGIDMDELRESINQTMTDVSGLIGRYSISNAEFDIEDVVQRAKVRQLTDLTDKLWELLKCCHSYKDLKIAFSMLFQCAARCNIVNTPTNKNRLAKIITELANRRLAMPCLSGAEPLELLLEIGLEKLYKDYEFIYTESKMCSTNLLKDDSSGASMDDGSPQNLPQLRKSLHNAVRGDPTPGAGMRKTLLHHHGAVNSRSTKYAGSDDDAGFKNSHFDEHESTERISKLFQIHCTLEHLLMMHIHLNLANVYNDVCSELLKKPPKLVESIDDQLSDVMDIRLSAHYVRDHLDGKDPYSRHITMRSHNKFRELKTTFYFNSENICPPNLAQCFQCDDKEMVKERTYHSWIYHKIRSLK

Target ORF information:

RefSeq Version	NM_170542.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster zwilch (Zwilch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170542.2

ORF Insert Sequence:

ATGTCGGCTT CTGCGAACTT GGCAAACGTT TACGCGGAGC TAATGCGGCG GTGTGGCGAG 
TCATACACGA TCACCTATGG AGCGCCACCC ACCTACTTGG TGAGCATGGT GGGGGCTGCG 
GAAGCTGGCA AGAAGATTGT GCTGGTCTTT AAGGAGGATC GCAATGGAGC AGTGGCGAGA 
CTCCGGACCA CGCCCACCAG GGCAGCCCCA AAAAAAGAGG GCTCTGCGGA CTTGGATCTG 
ACGGGAAGTC CTTTGAAGGA CGACTGCCTG GTGGACGCCA TAGCCGACTT GTCCATCGAC 
CTGCAGCTGG ATCACTCGAA TCCCTGGAAG CTGGAGGAGG AATATCAACG CGGAATACCC 
GTGGACAAGG CTAGGTCCAT CGTGTGCTCC GAGTTCCTGC AGCTGGCCGA GGGACTCGGA 
TCCGTGTGGT TTTTGTGCGA CGGCAGTGAT CTCGGGCAGA CTCAGTTGCT CCAGTACGAG 
TTCAACCCTA CGCACTTCTC CAGGGGAATA CTTAGCTACC AGGGTGTGCG CCCTGCTTAC 
TTAGTAACCT CGCAAGCCCT GGTGCGCCAT CATGGCAAGA CACCGGATGA AACTCTCATT 
GAAAATAGCT ACCAGGTCAA TCCCCACATG AGACTACGCT GCTCCTGGAC CTCTAGTGCT 
GCACTTCCGC TCCTGGTGAA CCTGAACGAC TGCGATGTGG CTTTGAATCA CACTTTTCGC 
GTGGGCGACT GCGGTCCTTT GACGCAGGAC TTTATGAATC AGCTGCGCAT TCTGGTCTAC 
ATACGCGAGG ACATCGTCTC CTACCACACG GATGTCAAGC AGGGCGTCTC TCGGGATCCC 
ACCTATCGTT GTGGCAGTGG CATTGACATG GACGAGCTGC GCGAGTCCAT TAATCAGACA 
ATGACAGATG TCTCTGGCCT TATAGGTCGG TATAGCATCA GCAATGCGGA GTTTGACATC 
GAAGATGTGG TGCAGAGGGC CAAGGTTCGC CAGCTCACGG ATCTAACCGA TAAGCTTTGG 
GAGCTGCTCA AGTGCTGCCA CTCCTACAAG GATCTCAAAA TTGCCTTTAG TATGCTCTTC 
CAATGTGCTG CACGGTGTAA CATAGTAAAC ACGCCTACTA ACAAAAATCG ACTTGCCAAG 
ATTATCACCG AGTTGGCCAA CCGTCGTCTG GCCATGCCTT GTTTAAGTGG GGCCGAGCCT 
CTGGAACTGC TTTTGGAAAT TGGCCTGGAG AAGTTGTACA AGGACTACGA GTTTATCTAC 
ACCGAGAGCA AGATGTGCAG CACCAATCTG TTGAAGGATG ACTCCAGCGG AGCGTCGATG 
GACGATGGTT CTCCCCAGAA TCTGCCCCAG CTTCGAAAAT CCCTGCACAA TGCGGTCAGG 
GGTGATCCGA CCCCTGGAGC TGGAATGCGA AAGACGCTGC TGCATCATCA CGGCGCTGTC 
AATTCGCGGA GTACAAAATA TGCCGGTAGC GACGATGATG CTGGGTTCAA AAACAGCCAT 
TTCGACGAGC ACGAGAGTAC GGAGAGAATC TCAAAGTTGT TTCAGATTCA CTGCACCTTA 
GAGCATCTCC TGATGATGCA CATCCACTTA AATCTGGCAA ATGTTTACAA CGATGTCTGT 
TCTGAATTGC TGAAGAAACC GCCTAAATTA GTAGAATCCA TCGATGATCA GCTAAGTGAT 
GTTATGGACA TTCGCCTGTC TGCCCACTAT GTGAGGGATC ACTTGGATGG CAAGGATCCC 
TACTCCCGGC ACATTACCAT GCGTTCGCAC AACAAGTTCC GCGAACTAAA GACCACCTTC 
TATTTCAATT CGGAAAACAT TTGTCCGCCG AACTTGGCTC AGTGTTTCCA GTGTGATGAC 
AAGGAGATGG TCAAGGAGCG CACATATCAT TCCTGGATAT ATCACAAGAT TCGCTCACTT 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Zwilch cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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