pgc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pgc
Entrez Gene ID	5740599
Full Name	polar granule component
Synonyms	CG11296,CG32885,CR32885,Dmel\CG32885,Pgc,Pgc1,pgc-1,pgr
General protein information	Preferred Names polar granule component Names CG32885 gene product from transcript CG32885-RA 58D gene CG32885-PA CG32885-PC CG32885-PD pgc-PA pgc-PC pgc-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 58D3-58D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103942.2	NP_001097412.1	polar granule component, isoform A
NM_001103941.2	NP_001097411.1	polar granule component, isoform C
NM_001299786.1	NP_001286715.1	polar granule component, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pgc embryos have greatly reduced levels of Nos protein.
Title: Maintaining sufficient nanos is a critical function for polar granule component in the specification of primordial germ cells.

lack of pgc partly ameliorates the cellularization defect of tor mutants, thus revealing a functional antagonism between pgc and tor in the specification of germline and somatic properties.
Title: A functional antagonism between the pgc germline repressor and torso in the development of somatic cells.

The following pgc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pgc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11920	NM_001299786.1 Latest version!	Drosophila melanogaster polar granule component (pgc), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11920	NM_001103942.2 Latest version!	Drosophila melanogaster polar granule component (pgc), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11920	NM_001103941.2 Latest version!	Drosophila melanogaster polar granule component (pgc), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pgc ( NM_001103942.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097412.1 Drosophila melanogaster polar granule component (pgc), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11920
Clone ID Related Accession (Same CDS sequence)	NM_001103942.2 , NM_001103941.2 , NM_001299786.1
Accession Version	NM_001103942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polar granule component, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103942.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGACT ACCAGATGGA GTACTCCTTT ATTTTTGAAG ACAGCTCCTG CGAGGGCGAT 
GCCTCGATGG CATCCTACGA CAATGGATTC GAGTCCATGT GGCATCAAGT GCGCGAGGAG 
TTGCAAAGGG AGCGGGAGAT GAATGAGCTC TGCCAGGTTT TCCAGCAAAA CTTGAGCCTG 
AGTCCGCCGG TCATCGCGGA TAGATGGAGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097412.1
CDS	595..810
Translation	MCDYQMEYSFIFEDSSCEGDASMASYDNGFESMWHQVREELQREREMNELCQVFQQNLSLSPPVIADRWRF

Target ORF information:

RefSeq Version	NM_001103942.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polar granule component (pgc), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103942.2

ORF Insert Sequence:

ATGTGCGACT ACCAGATGGA GTACTCCTTT ATTTTTGAAG ACAGCTCCTG CGAGGGCGAT 
GCCTCGATGG CATCCTACGA CAATGGATTC GAGTCCATGT GGCATCAAGT GCGCGAGGAG 
TTGCAAAGGG AGCGGGAGAT GAATGAGCTC TGCCAGGTTT TCCAGCAAAA CTTGAGCCTG 
AGTCCGCCGG TCATCGCGGA TAGATGGAGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pgc ( NM_001103941.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097411.1 Drosophila melanogaster polar granule component (pgc), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11920
Clone ID Related Accession (Same CDS sequence)	NM_001103942.2 , NM_001103941.2 , NM_001299786.1
Accession Version	NM_001103941.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polar granule component, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103941.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGACT ACCAGATGGA GTACTCCTTT ATTTTTGAAG ACAGCTCCTG CGAGGGCGAT 
GCCTCGATGG CATCCTACGA CAATGGATTC GAGTCCATGT GGCATCAAGT GCGCGAGGAG 
TTGCAAAGGG AGCGGGAGAT GAATGAGCTC TGCCAGGTTT TCCAGCAAAA CTTGAGCCTG 
AGTCCGCCGG TCATCGCGGA TAGATGGAGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097411.1
CDS	70..285
Translation	MCDYQMEYSFIFEDSSCEGDASMASYDNGFESMWHQVREELQREREMNELCQVFQQNLSLSPPVIADRWRF

Target ORF information:

RefSeq Version	NM_001103941.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polar granule component (pgc), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103941.2

ORF Insert Sequence:

ATGTGCGACT ACCAGATGGA GTACTCCTTT ATTTTTGAAG ACAGCTCCTG CGAGGGCGAT 
GCCTCGATGG CATCCTACGA CAATGGATTC GAGTCCATGT GGCATCAAGT GCGCGAGGAG 
TTGCAAAGGG AGCGGGAGAT GAATGAGCTC TGCCAGGTTT TCCAGCAAAA CTTGAGCCTG 
AGTCCGCCGG TCATCGCGGA TAGATGGAGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pgc ( NM_001299786.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286715.1 Drosophila melanogaster polar granule component (pgc), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11920
Clone ID Related Accession (Same CDS sequence)	NM_001103942.2 , NM_001103941.2 , NM_001299786.1
Accession Version	NM_001299786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polar granule component, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGACT ACCAGATGGA GTACTCCTTT ATTTTTGAAG ACAGCTCCTG CGAGGGCGAT 
GCCTCGATGG CATCCTACGA CAATGGATTC GAGTCCATGT GGCATCAAGT GCGCGAGGAG 
TTGCAAAGGG AGCGGGAGAT GAATGAGCTC TGCCAGGTTT TCCAGCAAAA CTTGAGCCTG 
AGTCCGCCGG TCATCGCGGA TAGATGGAGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286715.1
CDS	27..242
Translation	MCDYQMEYSFIFEDSSCEGDASMASYDNGFESMWHQVREELQREREMNELCQVFQQNLSLSPPVIADRWRF

Target ORF information:

RefSeq Version	NM_001299786.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polar granule component (pgc), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299786.1

ORF Insert Sequence:

ATGTGCGACT ACCAGATGGA GTACTCCTTT ATTTTTGAAG ACAGCTCCTG CGAGGGCGAT 
GCCTCGATGG CATCCTACGA CAATGGATTC GAGTCCATGT GGCATCAAGT GCGCGAGGAG 
TTGCAAAGGG AGCGGGAGAT GAATGAGCTC TGCCAGGTTT TCCAGCAAAA CTTGAGCCTG 
AGTCCGCCGG TCATCGCGGA TAGATGGAGA TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pgc cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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