gukh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gukh
Entrez Gene ID	53563
Full Name	GUK-holder
Synonyms	CG14287,CG14288,CG31043,CG5456,CG6003,Dmel\CG31043,GUKH,GUKh,GukH,Gukh,anon-WO0118547.205
General protein information	Preferred Names GUK-holder Names CG31043 gene product from transcript CG31043-RA CG31043-PA CG31043-PB CG31043-PC CG31043-PE GUK holder gukh-PA gukh-PB gukh-PC gukh-PE gukholder
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91E2-91E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001032021.2	NP_001027192.1	GUK-holder, isoform C
NM_001104364.2	NP_001097834.2	GUK-holder, isoform E
NM_169846.2	NP_732394.1	GUK-holder, isoform B
NM_169845.2	NP_732393.1	GUK-holder, isoform A

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001583	XP_321528.4
Drosophila melanogaster (fruit fly)	gukh	NP_001097834.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

multiple interdomain interactions in the scaffold Discs Large (Dlg) regulate binding to the synaptic protein GukHolder (GukH)
Title: Interdomain interactions in the tumor suppressor discs large regulate binding to the synaptic protein GukHolder.

The following gukh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gukh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08761	NM_169846.2 Latest version!	Drosophila melanogaster GUK-holder (gukh), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08760	NM_001032021.2 Latest version!	Drosophila melanogaster GUK-holder (gukh), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08759	NM_169845.2 Latest version!	Drosophila melanogaster GUK-holder (gukh), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa22288	NM_001104364.2 Latest version!	Drosophila melanogaster GUK-holder (gukh), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gukh ( NM_169846.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732394.1 Drosophila melanogaster GUK-holder (gukh), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08761
Clone ID Related Accession (Same CDS sequence)	NM_169846.2
Accession Version	NM_169846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GUK-holder, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169846.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGGGTA TCAACGTTTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732394.1
CDS	482..2086
Translation	MPFVQRVVQPVNVAQATAASLGHASGRFHCTPGKCRNNNCINKQSPDVEVPNGHGSPTSLTRVLVHQAPEDQPVPPAPAPMKKLKHHVEFLSTSPTRHQSQSLPNSRHPSQQRPALGRIASAPQSPTAGSSKVVSGHEFDSISNITLSNALRQLASLVLIASDIFDDLQRDLQAVGERAGRVQRKIAAVERRVCAYDPKTVTVPESDLLTFAQRKHHFETDKSFQQELFTGETRPLSVRQLYTEAGKELPVATGATATALASLAHSQSLIPSRSISFNGEVLSSDHEVLQLNGSTDTEDHLLCVSDFGNANRKLRTRIDAEIEIRLPAAIEDLRKWTSSEALGDVTVTPDCMHHVDTSVSTSLAIGENGILTPALSPSVLSADAVDALYAQNLSQAADSSRHNHHTQALIPNDINKDVPLNHRLPSPEEQTKQIALKYPAEVISVNTSGKHFQRMCAARKSTSGGYAGGATAGSSSSTSPENAGQTEGNNLDDNVQTVSRRSRSRKVRGKRRNTIAGIDQKEIQDAANGVSTFL

Target ORF information:

RefSeq Version	NM_169846.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GUK-holder (gukh), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169846.2

ORF Insert Sequence:

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGGGTA TCAACGTTTC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gukh ( NM_001032021.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027192.1 Drosophila melanogaster GUK-holder (gukh), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08760
Clone ID Related Accession (Same CDS sequence)	NM_001032021.2
Accession Version	NM_001032021.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GUK-holder, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001032021.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGTCTG AAGCGGCGCA AGGGTGCACA CACCACCTTC 
GCACCTGGGC CGTACGTGAA GGAAACCGAA AGTGCGCTCC ACAGTGCTGA AAGTGCAGCG 
GCGGCAATAG CATCGTCACA GGATCCATCA GCAGCATCAG GAGCCATTAG CAGTACCAAG 
AGCGCCAAGC TCCTACTGCT CACCAGCGGC GGATTAATCT CTGGCAATCG CAAGGTGGCC 
AACGTGAGGC CCATTGTGGC CAAATCCCCG CTCAGCAGGA ACGTCAGCGA AATGGCAACC 
TACCAGGCAA CCAGCTACCA GGACCAGAAA CAACTGCTGG CCAACCGAAC AAATCCCTTC 
TACGAGACCA TTGCCGCACC CGTGGCCAAT CTGATGATGC CCTCGCCGTC GGGCAAGTCC 
AATTCTTCGA CGGGCTCCAC AGCCACCAGT TCCTCCTCAT CGGCATCGGG TCAATCCGGT 
GAAGAAGGGT ATCAACGTTT CTCTAATATC TATGAGCAAG TGCAGCCAGC ACCGAGCACC 
CAGCCACAGG AAGATGCGAC TGCCACGCCC TTCCAGCGCA GTGCGCCCAT CTATTGGACA 
CTGCAGAATC GCCGCAGCCA GCCAAGGCCA CAGCCCGCTG GCAACGTGAC CTGCCGGGAT 
GACCTCTACG CCACGCCCAT TCGACGGGCC GACCGACTGC CACGCCCAGT GGCTGCTGCT 
GCGGCAACAG CAGCGGAAGG CAACAGGAGC AACATATCGC CAATTGTGCC AAGGAATCGT 
TCGGGAGCCT TCCTCACCTC CACGCCCACG AGGAGCGGAG AGGGTGAACG CGATTCGGAG 
AACACACAAC TTCCCCCCAT GAAGCAGCCA TCCCGCAACT GGACCGATGA CGCACCCGAA 
CGCCTCAACA CCTGTGCGGA TCGCATTGGC CAAAGCAAGA CCACGCTAAT GGACTTTAAG 
AAGCTGCTGC TGGCCAAGTC CGCCAAGGCG AGTCCGGTCC AAAGGAAGGT ATCCGCCGTG 
GAGCTGCTGA AGAAGCCAGC CCAAGCCACC GCAACGCCGC CCGTTGTAAA GCCCAGTGGC 
CCTGGACAGA AGGTAGCATC AGCTGGAAGC AAACCAACGA TCAACACCAG CCTAAAGCTG 
CTAGATCTGA GCGGCTCACC CAAGACTTTC GCCAACCGGA GGATGCTTCG GCAAGGCCAG 
TTTGGATCGC CCTCCAAGAC CTTTGCTCCA AAGATGCGAG GACCCGTGAA TATGGTGGCT 
GCTGCAGCGG CTCCACCGCG AACTGACATC ATGTCCACCA CGATTCCGGA GGCGAATAGC 
GAGGAGGATC ACTCCAACAC CAGCGGAGGA TCGCGAGCCA GTTCCGAGGC GGGCGAAACG 
GTGCCCTCCA GCTTCGACTT GAAGCGGAAC TTCTTTTTGC AAACCGAGGA GAACAATTTC 
ATGCGCGGGG AGCTGAAGCC CAGCTTTGCC AGGTCAAATG GAGCAGCCGG AGGTGGCAGC 
ACCGAAGGCG TCAACAAGTA CGTGGCAGCT CCGGTGGCGA ATCCTCCGCT GCCCTCGTTT 
GAAACGGCGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027192.1
CDS	482..3616
Translation	MPFVQRVVQPVNVAQATAASLGHASGRFHCTPGKCRNNNCINKQSPDVEVPNGHGSPTSLTRVLVHQAPEDQPVPPAPAPMKKLKHHVEFLSTSPTRHQSQSLPNSRHPSQQRPALGRIASAPQSPTAGSSKVVSGHEFDSISNITLSNALRQLASLVLIASDIFDDLQRDLQAVGERAGRVQRKIAAVERRVCAYDPKTVTVPESDLLTFAQRKHHFETDKSFQQELFTGETRPLSVRQLYTEAGKELPVATGATATALASLAHSQSLIPSRSISFNGEVLSSDHEVLQLNGSTDTEDHLLCVSDFGNANRKLRTRIDAEIEIRLPAAIEDLRKWTSSEALGDVTVTPDCMHHVDTSVSTSLAIGENGILTPALSPSVLSADAVDALYAQNLSQAADSSRHNHHTQALIPNDINKDVPLNHRLPSPEEQTKQIALKYPAEVISVNTSGKHFQRMCAARKSTSGGYAGGATAGSSSSTSPENAGQTEGNNLDDNVQTVSRRSRSRKVRGKRRNTIAGIDQKEIQDAANGLKRRKGAHTTFAPGPYVKETESALHSAESAAAAIASSQDPSAASGAISSTKSAKLLLLTSGGLISGNRKVANVRPIVAKSPLSRNVSEMATYQATSYQDQKQLLANRTNPFYETIAAPVANLMMPSPSGKSNSSTGSTATSSSSSASGQSGEEGYQRFSNIYEQVQPAPSTQPQEDATATPFQRSAPIYWTLQNRRSQPRPQPAGNVTCRDDLYATPIRRADRLPRPVAAAAATAAEGNRSNISPIVPRNRSGAFLTSTPTRSGEGERDSENTQLPPMKQPSRNWTDDAPERLNTCADRIGQSKTTLMDFKKLLLAKSAKASPVQRKVSAVELLKKPAQATATPPVVKPSGPGQKVASAGSKPTINTSLKLLDLSGSPKTFANRRMLRQGQFGSPSKTFAPKMRGPVNMVAAAAAPPRTDIMSTTIPEANSEEDHSNTSGGSRASSEAGETVPSSFDLKRNFFLQTEENNFMRGELKPSFARSNGAAGGGSTEGVNKYVAAPVANPPLPSFETAL

Target ORF information:

RefSeq Version	NM_001032021.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GUK-holder (gukh), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032021.2

ORF Insert Sequence:

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGTCTG AAGCGGCGCA AGGGTGCACA CACCACCTTC 
GCACCTGGGC CGTACGTGAA GGAAACCGAA AGTGCGCTCC ACAGTGCTGA AAGTGCAGCG 
GCGGCAATAG CATCGTCACA GGATCCATCA GCAGCATCAG GAGCCATTAG CAGTACCAAG 
AGCGCCAAGC TCCTACTGCT CACCAGCGGC GGATTAATCT CTGGCAATCG CAAGGTGGCC 
AACGTGAGGC CCATTGTGGC CAAATCCCCG CTCAGCAGGA ACGTCAGCGA AATGGCAACC 
TACCAGGCAA CCAGCTACCA GGACCAGAAA CAACTGCTGG CCAACCGAAC AAATCCCTTC 
TACGAGACCA TTGCCGCACC CGTGGCCAAT CTGATGATGC CCTCGCCGTC GGGCAAGTCC 
AATTCTTCGA CGGGCTCCAC AGCCACCAGT TCCTCCTCAT CGGCATCGGG TCAATCCGGT 
GAAGAAGGGT ATCAACGTTT CTCTAATATC TATGAGCAAG TGCAGCCAGC ACCGAGCACC 
CAGCCACAGG AAGATGCGAC TGCCACGCCC TTCCAGCGCA GTGCGCCCAT CTATTGGACA 
CTGCAGAATC GCCGCAGCCA GCCAAGGCCA CAGCCCGCTG GCAACGTGAC CTGCCGGGAT 
GACCTCTACG CCACGCCCAT TCGACGGGCC GACCGACTGC CACGCCCAGT GGCTGCTGCT 
GCGGCAACAG CAGCGGAAGG CAACAGGAGC AACATATCGC CAATTGTGCC AAGGAATCGT 
TCGGGAGCCT TCCTCACCTC CACGCCCACG AGGAGCGGAG AGGGTGAACG CGATTCGGAG 
AACACACAAC TTCCCCCCAT GAAGCAGCCA TCCCGCAACT GGACCGATGA CGCACCCGAA 
CGCCTCAACA CCTGTGCGGA TCGCATTGGC CAAAGCAAGA CCACGCTAAT GGACTTTAAG 
AAGCTGCTGC TGGCCAAGTC CGCCAAGGCG AGTCCGGTCC AAAGGAAGGT ATCCGCCGTG 
GAGCTGCTGA AGAAGCCAGC CCAAGCCACC GCAACGCCGC CCGTTGTAAA GCCCAGTGGC 
CCTGGACAGA AGGTAGCATC AGCTGGAAGC AAACCAACGA TCAACACCAG CCTAAAGCTG 
CTAGATCTGA GCGGCTCACC CAAGACTTTC GCCAACCGGA GGATGCTTCG GCAAGGCCAG 
TTTGGATCGC CCTCCAAGAC CTTTGCTCCA AAGATGCGAG GACCCGTGAA TATGGTGGCT 
GCTGCAGCGG CTCCACCGCG AACTGACATC ATGTCCACCA CGATTCCGGA GGCGAATAGC 
GAGGAGGATC ACTCCAACAC CAGCGGAGGA TCGCGAGCCA GTTCCGAGGC GGGCGAAACG 
GTGCCCTCCA GCTTCGACTT GAAGCGGAAC TTCTTTTTGC AAACCGAGGA GAACAATTTC 
ATGCGCGGGG AGCTGAAGCC CAGCTTTGCC AGGTCAAATG GAGCAGCCGG AGGTGGCAGC 
ACCGAAGGCG TCAACAAGTA CGTGGCAGCT CCGGTGGCGA ATCCTCCGCT GCCCTCGTTT 
GAAACGGCGT TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gukh ( NM_169845.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732393.1 Drosophila melanogaster GUK-holder (gukh), transcript variant A, mRNA.

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CloneID	OFa08759
Clone ID Related Accession (Same CDS sequence)	NM_169845.2
Accession Version	NM_169845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GUK-holder, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169845.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGCAAT GGGGAGTCCA AGAATGCCTC AAATGCCACA 
TCTCCGGTGG CTCCAGCTCA GCAGGCGGAG ACCACCTCGT CGGATGCCAA GAAGTCCAAG 
AAGTTCGGAC GCAGCAAGTC CAGTGACATA TTAAAAAAGG AATCTGCTGC CTTGCCCTAC 
GACAAGGGAA AGAGCACCCT CACCCGGCTG AATTCCCTGA AGCAGTGGGG TCGCAACCGC 
TTCAAGTTCA TGCATCGTAC CGGCGAACTG GGCGACCAGT TGGACACCAG CGGCTGCAGC 
TCCCAGAACA GTTCCGCGGA GAAGGCGGAC AGCCATCATG GTTCGCCGGC GGGCGACAAT 
TCCAAACGCG ACATCGACGA CGAATGCGTG GTGCATCATC TGGTGGCCCA GAAGAGTGAA 
TCTCGGTTTT CGCACGAACG AAAGCCCTCG TATTCCTCCT CGGAGAAGAG CATTAGTGTG 
TCCAGCAGTG GCCAGCTCAG GGGCAGACTG CAATTATCAT CCGGACCGAT GGCAGCGGCC 
CATGTAAAGA TGCGGGAAAC AGCCACTCTG AACAGGCAGC GACGAAACGG TCTACATGCA 
CTGGCTGGCA AGGAGGAGCA GCCGCATTCC TCAAGCGGCA ACTGGAGTGC CAGCTCGGAA 
TCGGGACGCG CCTCCATTGG CAGCGAGATC ACCATGCAGC CGAAGTCGAG TGCCTCGAGT 
ACATCGTTAA ATGTATCCAG TTTTCAGCCA GGAGGAGCGG GCAGTGGCCC ACCATCCTCC 
ATGCTGAGCA GAAGGAGATT CCTGAACACC TCCGCCTCCA GCAGTGTGAC GAGTGAGGGC 
ACTGCCACGC CGGAACTGCA GGCGGAGGCA GGTGCCTATC CAGGTCACCT AGATGATGAG 
ACAAGTTCCG CTTACAGCTG CGACACAGAG GGCTACTACA CATCCTTCCA CATGGACAGC 
GGTCTTCGAA CGCTGAAGGA AGAGGAGCCA TCCGTGATGC CCGGTACCCC GCTCCAGACG 
AGCCTGCACA CCAGCAGCTA CTCCTTCGGT AGCCAGTCCA ATCAAACAGT GCTCAATGCG 
GAGAGCGAGT ATGAGCTGTT CGGCAGAGGA TCCACCTCCA CGACAACGAG CTCCGCGGGC 
ACCGTGTGCA CAGCTCTGAT GAGCGGTGCC GCCTCTGGGC CGGATGTGCC CGAGCGGAGT 
AGCTCGCTGG GCAAGCACAG TGGTCAGAGC CGGAGCCAGA GCACCAGTGC CAGCAGCAGC 
ACGCTGGAGA GGAGCTACAG CAGCAGTACC ATCGGAAGCA CTCTGGAGCG CACGGGAACT 
ATCAAGAGGA ATGTCAAGAT GTCGCCAACC AAGGCAGGTG GTGAAGTGAC GGAGAAGGAT 
GACCAGCAGG ACCAGGATCA GCAACAGCAG CTTGAGTTCT CCGAGAGCTC CGATCTGGAG 
GGCGTGGAAC GCATAGAGCG GATCAAGCAG AAGACGGCCC TCACCTCCAA GAGAATACCC 
TCGATGTGCA TCATTACGCC CACAACGAGT GACGACGATG AGCACCAGAC CAATGATCCG 
AATCGCACGC TCACCAGTCT CACCATCGAT GTGGAGCACG GGGAGCAGGA CGAGGATGAG 
GCGGAGCAAA AGACGCCCAC GAATAGCAAG GACCACAACC TAAACGAACT GAAGCAGACG 
CCCACGAAAG CGCTGAGCGG GATGTTCGAG AAACTGAGGG TGAAACTGGT TCCGGGCAAA 
AGCTCTCCGG CCAAGAATCA AATGCAACCC AAGCCCGCCA ACTCGAGTGC CGTGAATGAC 
GATGAGCTTT ACGATTTGGC CGGCGAGTAC GTCACCATAG CAGACATCAG CAACAACAAC 
AACAACAACA AGAACATTGG GCGACGTAAC ACGGGAATGG GTATATACTA TTCCAATGAC 
ATAGTTTCCA AGAGGGTTGA ACCATCGACG TCTGCCGTTT CCGAGTACGT TTCGCTAAAC 
GAGCTGCCAC AGCACTTGCG TCGGCACGCG GTCTCGTCGA GATCCGCTCC TGCAGCGGAG 
AGCGAGTCCT GCGCACAACA ACAGCTGCCC GTCGAGCCGG CAGCTGCGAC GTCCCAACCA 
CCACCACTAC CCCTCGGCGG CAGGACGCAG GGGGTGGGCG ATGGGGTGCC CATGTACGCG 
GAAATCGGCG AATTGAGCGA AGAGAAGCCG CTCGCATCGG CGGCAAATCG TCGACTGCGC 
CCGAATGCCG AGGGGAAATT GGTCTACGAC TCCGACAGTC TGAAGCGGCG CAAGGGTGCA 
CACACCACCT TCGCACCTGG GCCGTACGTG AAGGAAACCG AAAGTGCGCT CCACAGTGCT 
GAAAGTGCAG CGGCGGCAAT AGCATCGTCA CAGGATCCAT CAGCAGCATC AGGAGCCATT 
AGCAGTACCA AGAGCGCCAA GCTCCTACTG CTCACCAGCG GCGGATTAAT CTCTGGCAAT 
CGCAAGGTGG CCAACGTGAG GCCCATTGTG GCCAAATCCC CGCTCAGCAG GAACGTCAGC 
GAAATGGCAA CCTACCAGGC AACCAGCTAC CAGGACCAGA AACAACTGCT GGCCAACCGA 
ACAAATCCCT TCTACGAGAC CATTGCCGCA CCCGTGGCCA ATCTGATGAT GCCCTCGCCG 
TCGGGCAAGT CCAATTCTTC GACGGGCTCC ACAGCCACCA GTTCCTCCTC ATCGGCATCG 
GGTCAATCCG GTGAAGAAGG GTATCAACGT TTCTCTAATA TCTATGAGCA AGTGCAGCCA 
GCACCGAGCA CCCAGCCACA GGAAGATGCG ACTGCCACGC CCTTCCAGCG CAGTGCGCCC 
ATCTATTGGA CACTGCAGAA TCGCCGCAGC CAGCCAAGGC CACAGCCCGC TGGCAACGTG 
ACCTGCCGGG ATGACCTCTA CGCCACGCCC ATTCGACGGG CCGACCGACT GCCACGCCCA 
GTGGCTGCTG CTGCGGCAAC AGCAGCGGAA GGCAACAGGA GCAACATATC GCCAATTGTG 
CCAAGGAATC GTTCGGGAGC CTTCCTCACC TCCACGCCCA CGAGGAGCGG AGAGGGTGAA 
CGCGATTCGG AGAACACACA ACTTCCCCCC ATGAAGCAGC CATCCCGCAA CTGGACCGAT 
GACGCACCCG AACGCCTCAA CACCTGTGCG GATCGCATTG GCCAAAGCAA GACCACGCTA 
ATGGACTTTA AGAAGCTGCT GCTGGCCAAG TCCGCCAAGG CGAGTCCGGT CCAAAGGAAG 
GTATCCGCCG TGGAGCTGCT GAAGAAGCCA GCCCAAGCCA CCGCAACGCC GCCCGTTGTA 
AAGCCCAGTG GCCCTGGACA GAAGGTAGCA TCAGCTGGAA GCAAACCAAC GATCAACACC 
AGCCTAAAGC TGCTAGATCT GAGCGGCTCA CCCAAGACTT TCGCCAACCG GAGGATGCTT 
CGGCAAGGCC AGTTTGGATC GCCCTCCAAG ACCTTTGCTC CAAAGATGCG AGGACCCGTG 
AATATGGTGG CTGCTGCAGC GGCTCCACCG CGAACTGACA TCATGTCCAC CACGATTCCG 
GAGGCGAATA GCGAGGAGGA TCACTCCAAC ACCAGCGGAG GATCGCGAGC CAGTTCCGAG 
GCGGGCGAAA CGGTGCCCTC CAGCTTCGAC TTGAAGCGGA ACTTCTTTTT GCAAACCGAG 
GAGAACAATT TCATGCGCGG GGAGCTGAAG CCCAGCTTTG CCAGGTCAAA TGGAGCAGCC 
GGAGGTGGCA GCACCGAAGG CGTCAACAAG TACGTGGCAG CTCCGGTGGC GAATCCTCCG 
CTGCCCTCGT TTGAAACGGC GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732393.1
CDS	482..5848
Translation	MPFVQRVVQPVNVAQATAASLGHASGRFHCTPGKCRNNNCINKQSPDVEVPNGHGSPTSLTRVLVHQAPEDQPVPPAPAPMKKLKHHVEFLSTSPTRHQSQSLPNSRHPSQQRPALGRIASAPQSPTAGSSKVVSGHEFDSISNITLSNALRQLASLVLIASDIFDDLQRDLQAVGERAGRVQRKIAAVERRVCAYDPKTVTVPESDLLTFAQRKHHFETDKSFQQELFTGETRPLSVRQLYTEAGKELPVATGATATALASLAHSQSLIPSRSISFNGEVLSSDHEVLQLNGSTDTEDHLLCVSDFGNANRKLRTRIDAEIEIRLPAAIEDLRKWTSSEALGDVTVTPDCMHHVDTSVSTSLAIGENGILTPALSPSVLSADAVDALYAQNLSQAADSSRHNHHTQALIPNDINKDVPLNHRLPSPEEQTKQIALKYPAEVISVNTSGKHFQRMCAARKSTSGGYAGGATAGSSSSTSPENAGQTEGNNLDDNVQTVSRRSRSRKVRGKRRNTIAGIDQKEIQDAANGNGESKNASNATSPVAPAQQAETTSSDAKKSKKFGRSKSSDILKKESAALPYDKGKSTLTRLNSLKQWGRNRFKFMHRTGELGDQLDTSGCSSQNSSAEKADSHHGSPAGDNSKRDIDDECVVHHLVAQKSESRFSHERKPSYSSSEKSISVSSSGQLRGRLQLSSGPMAAAHVKMRETATLNRQRRNGLHALAGKEEQPHSSSGNWSASSESGRASIGSEITMQPKSSASSTSLNVSSFQPGGAGSGPPSSMLSRRRFLNTSASSSVTSEGTATPELQAEAGAYPGHLDDETSSAYSCDTEGYYTSFHMDSGLRTLKEEEPSVMPGTPLQTSLHTSSYSFGSQSNQTVLNAESEYELFGRGSTSTTTSSAGTVCTALMSGAASGPDVPERSSSLGKHSGQSRSQSTSASSSTLERSYSSSTIGSTLERTGTIKRNVKMSPTKAGGEVTEKDDQQDQDQQQQLEFSESSDLEGVERIERIKQKTALTSKRIPSMCIITPTTSDDDEHQTNDPNRTLTSLTIDVEHGEQDEDEAEQKTPTNSKDHNLNELKQTPTKALSGMFEKLRVKLVPGKSSPAKNQMQPKPANSSAVNDDELYDLAGEYVTIADISNNNNNNKNIGRRNTGMGIYYSNDIVSKRVEPSTSAVSEYVSLNELPQHLRRHAVSSRSAPAAESESCAQQQLPVEPAAATSQPPPLPLGGRTQGVGDGVPMYAEIGELSEEKPLASAANRRLRPNAEGKLVYDSDSLKRRKGAHTTFAPGPYVKETESALHSAESAAAAIASSQDPSAASGAISSTKSAKLLLLTSGGLISGNRKVANVRPIVAKSPLSRNVSEMATYQATSYQDQKQLLANRTNPFYETIAAPVANLMMPSPSGKSNSSTGSTATSSSSSASGQSGEEGYQRFSNIYEQVQPAPSTQPQEDATATPFQRSAPIYWTLQNRRSQPRPQPAGNVTCRDDLYATPIRRADRLPRPVAAAAATAAEGNRSNISPIVPRNRSGAFLTSTPTRSGEGERDSENTQLPPMKQPSRNWTDDAPERLNTCADRIGQSKTTLMDFKKLLLAKSAKASPVQRKVSAVELLKKPAQATATPPVVKPSGPGQKVASAGSKPTINTSLKLLDLSGSPKTFANRRMLRQGQFGSPSKTFAPKMRGPVNMVAAAAAPPRTDIMSTTIPEANSEEDHSNTSGGSRASSEAGETVPSSFDLKRNFFLQTEENNFMRGELKPSFARSNGAAGGGSTEGVNKYVAAPVANPPLPSFETAL

Target ORF information:

RefSeq Version	NM_169845.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GUK-holder (gukh), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169845.2

ORF Insert Sequence:

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGCAAT GGGGAGTCCA AGAATGCCTC AAATGCCACA 
TCTCCGGTGG CTCCAGCTCA GCAGGCGGAG ACCACCTCGT CGGATGCCAA GAAGTCCAAG 
AAGTTCGGAC GCAGCAAGTC CAGTGACATA TTAAAAAAGG AATCTGCTGC CTTGCCCTAC 
GACAAGGGAA AGAGCACCCT CACCCGGCTG AATTCCCTGA AGCAGTGGGG TCGCAACCGC 
TTCAAGTTCA TGCATCGTAC CGGCGAACTG GGCGACCAGT TGGACACCAG CGGCTGCAGC 
TCCCAGAACA GTTCCGCGGA GAAGGCGGAC AGCCATCATG GTTCGCCGGC GGGCGACAAT 
TCCAAACGCG ACATCGACGA CGAATGCGTG GTGCATCATC TGGTGGCCCA GAAGAGTGAA 
TCTCGGTTTT CGCACGAACG AAAGCCCTCG TATTCCTCCT CGGAGAAGAG CATTAGTGTG 
TCCAGCAGTG GCCAGCTCAG GGGCAGACTG CAATTATCAT CCGGACCGAT GGCAGCGGCC 
CATGTAAAGA TGCGGGAAAC AGCCACTCTG AACAGGCAGC GACGAAACGG TCTACATGCA 
CTGGCTGGCA AGGAGGAGCA GCCGCATTCC TCAAGCGGCA ACTGGAGTGC CAGCTCGGAA 
TCGGGACGCG CCTCCATTGG CAGCGAGATC ACCATGCAGC CGAAGTCGAG TGCCTCGAGT 
ACATCGTTAA ATGTATCCAG TTTTCAGCCA GGAGGAGCGG GCAGTGGCCC ACCATCCTCC 
ATGCTGAGCA GAAGGAGATT CCTGAACACC TCCGCCTCCA GCAGTGTGAC GAGTGAGGGC 
ACTGCCACGC CGGAACTGCA GGCGGAGGCA GGTGCCTATC CAGGTCACCT AGATGATGAG 
ACAAGTTCCG CTTACAGCTG CGACACAGAG GGCTACTACA CATCCTTCCA CATGGACAGC 
GGTCTTCGAA CGCTGAAGGA AGAGGAGCCA TCCGTGATGC CCGGTACCCC GCTCCAGACG 
AGCCTGCACA CCAGCAGCTA CTCCTTCGGT AGCCAGTCCA ATCAAACAGT GCTCAATGCG 
GAGAGCGAGT ATGAGCTGTT CGGCAGAGGA TCCACCTCCA CGACAACGAG CTCCGCGGGC 
ACCGTGTGCA CAGCTCTGAT GAGCGGTGCC GCCTCTGGGC CGGATGTGCC CGAGCGGAGT 
AGCTCGCTGG GCAAGCACAG TGGTCAGAGC CGGAGCCAGA GCACCAGTGC CAGCAGCAGC 
ACGCTGGAGA GGAGCTACAG CAGCAGTACC ATCGGAAGCA CTCTGGAGCG CACGGGAACT 
ATCAAGAGGA ATGTCAAGAT GTCGCCAACC AAGGCAGGTG GTGAAGTGAC GGAGAAGGAT 
GACCAGCAGG ACCAGGATCA GCAACAGCAG CTTGAGTTCT CCGAGAGCTC CGATCTGGAG 
GGCGTGGAAC GCATAGAGCG GATCAAGCAG AAGACGGCCC TCACCTCCAA GAGAATACCC 
TCGATGTGCA TCATTACGCC CACAACGAGT GACGACGATG AGCACCAGAC CAATGATCCG 
AATCGCACGC TCACCAGTCT CACCATCGAT GTGGAGCACG GGGAGCAGGA CGAGGATGAG 
GCGGAGCAAA AGACGCCCAC GAATAGCAAG GACCACAACC TAAACGAACT GAAGCAGACG 
CCCACGAAAG CGCTGAGCGG GATGTTCGAG AAACTGAGGG TGAAACTGGT TCCGGGCAAA 
AGCTCTCCGG CCAAGAATCA AATGCAACCC AAGCCCGCCA ACTCGAGTGC CGTGAATGAC 
GATGAGCTTT ACGATTTGGC CGGCGAGTAC GTCACCATAG CAGACATCAG CAACAACAAC 
AACAACAACA AGAACATTGG GCGACGTAAC ACGGGAATGG GTATATACTA TTCCAATGAC 
ATAGTTTCCA AGAGGGTTGA ACCATCGACG TCTGCCGTTT CCGAGTACGT TTCGCTAAAC 
GAGCTGCCAC AGCACTTGCG TCGGCACGCG GTCTCGTCGA GATCCGCTCC TGCAGCGGAG 
AGCGAGTCCT GCGCACAACA ACAGCTGCCC GTCGAGCCGG CAGCTGCGAC GTCCCAACCA 
CCACCACTAC CCCTCGGCGG CAGGACGCAG GGGGTGGGCG ATGGGGTGCC CATGTACGCG 
GAAATCGGCG AATTGAGCGA AGAGAAGCCG CTCGCATCGG CGGCAAATCG TCGACTGCGC 
CCGAATGCCG AGGGGAAATT GGTCTACGAC TCCGACAGTC TGAAGCGGCG CAAGGGTGCA 
CACACCACCT TCGCACCTGG GCCGTACGTG AAGGAAACCG AAAGTGCGCT CCACAGTGCT 
GAAAGTGCAG CGGCGGCAAT AGCATCGTCA CAGGATCCAT CAGCAGCATC AGGAGCCATT 
AGCAGTACCA AGAGCGCCAA GCTCCTACTG CTCACCAGCG GCGGATTAAT CTCTGGCAAT 
CGCAAGGTGG CCAACGTGAG GCCCATTGTG GCCAAATCCC CGCTCAGCAG GAACGTCAGC 
GAAATGGCAA CCTACCAGGC AACCAGCTAC CAGGACCAGA AACAACTGCT GGCCAACCGA 
ACAAATCCCT TCTACGAGAC CATTGCCGCA CCCGTGGCCA ATCTGATGAT GCCCTCGCCG 
TCGGGCAAGT CCAATTCTTC GACGGGCTCC ACAGCCACCA GTTCCTCCTC ATCGGCATCG 
GGTCAATCCG GTGAAGAAGG GTATCAACGT TTCTCTAATA TCTATGAGCA AGTGCAGCCA 
GCACCGAGCA CCCAGCCACA GGAAGATGCG ACTGCCACGC CCTTCCAGCG CAGTGCGCCC 
ATCTATTGGA CACTGCAGAA TCGCCGCAGC CAGCCAAGGC CACAGCCCGC TGGCAACGTG 
ACCTGCCGGG ATGACCTCTA CGCCACGCCC ATTCGACGGG CCGACCGACT GCCACGCCCA 
GTGGCTGCTG CTGCGGCAAC AGCAGCGGAA GGCAACAGGA GCAACATATC GCCAATTGTG 
CCAAGGAATC GTTCGGGAGC CTTCCTCACC TCCACGCCCA CGAGGAGCGG AGAGGGTGAA 
CGCGATTCGG AGAACACACA ACTTCCCCCC ATGAAGCAGC CATCCCGCAA CTGGACCGAT 
GACGCACCCG AACGCCTCAA CACCTGTGCG GATCGCATTG GCCAAAGCAA GACCACGCTA 
ATGGACTTTA AGAAGCTGCT GCTGGCCAAG TCCGCCAAGG CGAGTCCGGT CCAAAGGAAG 
GTATCCGCCG TGGAGCTGCT GAAGAAGCCA GCCCAAGCCA CCGCAACGCC GCCCGTTGTA 
AAGCCCAGTG GCCCTGGACA GAAGGTAGCA TCAGCTGGAA GCAAACCAAC GATCAACACC 
AGCCTAAAGC TGCTAGATCT GAGCGGCTCA CCCAAGACTT TCGCCAACCG GAGGATGCTT 
CGGCAAGGCC AGTTTGGATC GCCCTCCAAG ACCTTTGCTC CAAAGATGCG AGGACCCGTG 
AATATGGTGG CTGCTGCAGC GGCTCCACCG CGAACTGACA TCATGTCCAC CACGATTCCG 
GAGGCGAATA GCGAGGAGGA TCACTCCAAC ACCAGCGGAG GATCGCGAGC CAGTTCCGAG 
GCGGGCGAAA CGGTGCCCTC CAGCTTCGAC TTGAAGCGGA ACTTCTTTTT GCAAACCGAG 
GAGAACAATT TCATGCGCGG GGAGCTGAAG CCCAGCTTTG CCAGGTCAAA TGGAGCAGCC 
GGAGGTGGCA GCACCGAAGG CGTCAACAAG TACGTGGCAG CTCCGGTGGC GAATCCTCCG 
CTGCCCTCGT TTGAAACGGC GTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gukh ( NM_001104364.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097834.2 Drosophila melanogaster GUK-holder (gukh), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa22288
Clone ID Related Accession (Same CDS sequence)	NM_001104364.2
Accession Version	NM_001104364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	GUK-holder, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001104364.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGCAAT GGGGAGTCCA AGAATGCCTC AAATGCCACA 
TCTCCGGTGG CTCCAGCTCA GCAGGCGGAG ACCACCTCGT CGGATGCCAA GAAGTCCAAG 
AAGTTCGGAC GCAGCAAGTC CAGTGACATA TTAAAAAAGG AATCTGCTGC CTTGCCCTAC 
GACAAGGGAA AGAGCACCCT CACCCGGCTG AATTCCCTGA AGCAGTGGGG TCGCAACCGC 
TTCAAGTTCA TGCATCGTAC CGGCGAACTG GGCGACCAGT TGGACACCAG CGGCTGCAGC 
TCCCAGAACA GTTCCGCGGA GAAGGCGGAC AGCCATCATG GTTCGCCGGC GGGCGACAAT 
TCCAAACGCG ACATCGACGA CGAATGCGTG GTGCATCATC TGGTGGCCCA GAAGAGTGAA 
TCTCGGTTTT CGCACGAACG AAAGCCCTCG TATTCCTCCT CGGAGAAGAG CATTAGTGTG 
TCCAGCAGTG GCCAGCTCAG GGGCAGACTG CAATTATCAT CCGGACCGAT GGCAGCGGCC 
CATGTAAAGA TGCGGGAAAC AGCCACTCTG AACAGGCAGC GACGAAACGG TCTACATGCA 
CTGGCTGGCA AGGAGGAGCA GCCGCATTCC TCAAGCGGCA ACTGGAGTGC CAGCTCGGAA 
TCGGGACGCG CCTCCATTGG CAGCGAGATC ACCATGCAGC CGAAGTCGAG TGCCTCGAGT 
ACATCGTTAA ATGTATCCAG TTTTCAGCCA GGAGGAGCGG GCAGTGGCCC ACCATCCTCC 
ATGCTGAGCA GAAGGAGATT CCTGAACACC TCCGCCTCCA GCAGTGTGAC GAGTGAGGGC 
ACTGCCACGC CGGAACTGCA GGCGGAGGCA GGTGCCTATC CAGGTCACCT AGATGATGAG 
ACAAGTTCCG CTTACAGCTG CGACACAGAG GGCTACTACA CATCCTTCCA CATGGACAGC 
GGTCTTCGAA CGCTGAAGGA AGAGGAGCCA TCCGTGATGC CCGGTACCCC GCTCCAGACG 
AGCCTGCACA CCAGCAGCTA CTCCTTCGGT AGCCAGTCCA ATCAAACAGT GCTCAATGCG 
GAGAGCGAGT ATGAGCTGTT CGGCAGAGGA TCCACCTCCA CGACAACGAG CTCCGCGGGC 
ACCGTGTGCA CAGCTCTGAT GAGCGGTGCC GCCTCTGGGC CGGATGTGCC CGAGCGGAGT 
AGCTCGCTGG GCAAGCACAG TGGTCAGAGC CGGAGCCAGA GCACCAGTGC CAGCAGCAGC 
ACGCTGGAGA GGAGCTACAG CAGCAGTACC ATCGGAAGCA CTCTGGAGCG CACGGGAACT 
ATCAAGAGGA ATGTCAAGAT GTCGCCAACC AAGGCAGGTG GTGAAGTGAC GGAGAAGGAT 
GACCAGCAGG ACCAGGATCA GCAACAGCAG CTTGAGTTCT CCGAGAGCTC CGATCTGGAG 
GGCGTGGAAC GCATAGAGCG GATCAAGCAG AAGACGGCCC TCACCTCCAA GAGAATACCC 
TCGATGTGCA TCATTACGCC CACAACGAGT GACGACGATG AGCACCAGAC CAATGATCCG 
AATCGCACGC TCACCAGTCT CACCATCGAT GTGGAGCACG GGGAGCAGGA CGAGGATGAG 
GCGGAGCAAA AGACGCCCAC GAATAGCAAG GACCACAACC TAAACGAACT GAAGCAGACG 
CCCACGAAAG CGCTGAGCGG GATGTTCGAG AAACTGAGGG TGAAACTGGT TCCGGGCAAA 
AGCTCTCCGG CCAAGAATCA AATGCAACCC AAGCCCGCCA ACTCGAGTGC CGTGAATGAC 
GATGAGCTTT ACGATTTGGC CGGCGAGTAC GTCACCATAG CAGACATCAG CAACAACAAC 
AACAACAACA AGAACATTGG GCGACGTAAC ACGGGAATGG GTATATACTA TTCCAATGAC 
ATAGTTTCCA AGAGGGTTGA ACCATCGACG TCTGCCGTTT CCGAGTACGT TTCGCTAAAC 
GAGCTGCCAC AGCACTTGCG TCGGCACGCG GTCTCGTCGA GATCCGCTCC TGCAGCGGAG 
AGCGAGTCCT GCGCACAACA ACAGCTGCCC GTCGAGCCGG CAGCTGCGAC GTCCCAACCA 
CCACCACTAC CCCTCGGCGG CAGGACGCAG GGGGTGGGCG ATGGGGTGCC CATGTACGCG 
GAAATCGGCG AATTGAGCGA AGAGAAGCCG CTCGCATCGG CGGCAAATCG TCGACTGCGC 
CCGAATGCCG AGGGGAAATT GGTCTACGAC TCCGACAGTC TGAAGCGGCG CAAGGGTGCA 
CACACCACCT TCGCACCTGG GCCGTACGTG AAGGAAACCG AAAGTGCGCT CCACAGTGCT 
GAAAGTGCAG CGGCGGCAAT AGCATCGTCA CAGGATCCAT CAGCAGCATC AGGAGCCATT 
AGCAGTACCA AGAGCGCCAA GCTCCTACTG CTCACCAGCG GCGGATTAAT CTCTGGCAAT 
CGCAAGGTGG CCAACGTGAG GCCCATTGTG GCCAAATCCC CGCTCAGCAG GAACGTCAGC 
GAAATGGCAA CCTACCAGGC AACCAGCTAC CAGGACCAGA AACAACTGCT GGCCAACCGA 
ACAAATCCCT TCTACGAGAC CATTGCCGCA CCCGTGGCCA ATCTGATGAT GCCCTCGCCG 
TCGGGCAAGT CCAATTCTTC GACGGGCTCC ACAGCCACCA GTTCCTCCTC ATCGGCATCG 
GGTCAATCCG GTGAAGAAGG TAAGCCAGAG CGGCTCCCGT CAGCCAGCAG CCGCCCCTCA 
GCTTGCTTAC CCACAACTAA ATCTCAATCC GAATCCCTAT CCTTATCTGC ATCCGGTTTC 
CCTGTAAATA ACACTGCCAC CACTGAAACG TCTGCCAAGA GCACCCTTAA TCACCACATA 
GCCACCAAGT CAGCGGGCAG CATATCAAGT GTAAGGGAGC ATCAGCCCCA GCATCCAAAT 
CAGAACCCCA AAGATGGCGA GTCGAAAGTG AGGGTTGTTG AAATGGAAAC TACCACCCTG 
GGGGATGCGT TCGAAATGGG GGAACAGCAA GGGCCACCCC TTCCGCCGAG GGCGAAAGTT 
AACGACAAAC AGCACCCCAC CAAGCCCCTA TCGATTGACC CAATTGCGCT GCACTTTTCC 
ATCGCTGGCA ATCCCGGCCA GCCGCGCACC GCACTCAAGC GGAACAACAG CTACCGGTTG 
GCCAACGATG AGCTCAAACT GGAGATGAAC AAGGAGAATC TCTCCGTGTC CTTCGGCTCC 
GAGAGAGCAC CTAGTTCGGG TGCAATGCCC CTAAGTTCCG TGCATCAGCA GCACGCAACA 
AGTTCCCTTA GGCTGCGCAA CCTGAGCAAC CACCTGCCCA CCAACACCCA CTCACCTGGC 
GGGGAGCGGC GGATAGCCAA GTCCTTTGAG CGCCTGGTGG ACGAGTTCTC GCTGTCGTTA 
ACCACGGGTA ACGATTCCTC CTATCTGAAG AACAAGTCGC TCGAGAACTT TGATGACTCA 
CCAGCCCTGT TGTCCGTAGC TTCGCCTGAA CAAAGGCCAG ACAAGAACGC CTCAAACTCC 
TCAGATTCCG AATCGGAGGA TGACGAGGAG GAGTTGGCCC CGCCTCTGTC CCAGTCCCTG 
CCAGCCCCAA AGTACCACAA GTCCTTTGTG GACAGTGTGC TGCTGCGCCC GTTCAAAATC 
ATCCTGCCCA GCGATGAGAA CGGCAACCGT CGCCAGCGCA TTCTACAGAA ATTCGAGGTT 
ACCGAGATAT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097834.2
CDS	482..5776
Translation	MPFVQRVVQPVNVAQATAASLGHASGRFHCTPGKCRNNNCINKQSPDVEVPNGHGSPTSLTRVLVHQAPEDQPVPPAPAPMKKLKHHVEFLSTSPTRHQSQSLPNSRHPSQQRPALGRIASAPQSPTAGSSKVVSGHEFDSISNITLSNALRQLASLVLIASDIFDDLQRDLQAVGERAGRVQRKIAAVERRVCAYDPKTVTVPESDLLTFAQRKHHFETDKSFQQELFTGETRPLSVRQLYTEAGKELPVATGATATALASLAHSQSLIPSRSISFNGEVLSSDHEVLQLNGSTDTEDHLLCVSDFGNANRKLRTRIDAEIEIRLPAAIEDLRKWTSSEALGDVTVTPDCMHHVDTSVSTSLAIGENGILTPALSPSVLSADAVDALYAQNLSQAADSSRHNHHTQALIPNDINKDVPLNHRLPSPEEQTKQIALKYPAEVISVNTSGKHFQRMCAARKSTSGGYAGGATAGSSSSTSPENAGQTEGNNLDDNVQTVSRRSRSRKVRGKRRNTIAGIDQKEIQDAANGNGESKNASNATSPVAPAQQAETTSSDAKKSKKFGRSKSSDILKKESAALPYDKGKSTLTRLNSLKQWGRNRFKFMHRTGELGDQLDTSGCSSQNSSAEKADSHHGSPAGDNSKRDIDDECVVHHLVAQKSESRFSHERKPSYSSSEKSISVSSSGQLRGRLQLSSGPMAAAHVKMRETATLNRQRRNGLHALAGKEEQPHSSSGNWSASSESGRASIGSEITMQPKSSASSTSLNVSSFQPGGAGSGPPSSMLSRRRFLNTSASSSVTSEGTATPELQAEAGAYPGHLDDETSSAYSCDTEGYYTSFHMDSGLRTLKEEEPSVMPGTPLQTSLHTSSYSFGSQSNQTVLNAESEYELFGRGSTSTTTSSAGTVCTALMSGAASGPDVPERSSSLGKHSGQSRSQSTSASSSTLERSYSSSTIGSTLERTGTIKRNVKMSPTKAGGEVTEKDDQQDQDQQQQLEFSESSDLEGVERIERIKQKTALTSKRIPSMCIITPTTSDDDEHQTNDPNRTLTSLTIDVEHGEQDEDEAEQKTPTNSKDHNLNELKQTPTKALSGMFEKLRVKLVPGKSSPAKNQMQPKPANSSAVNDDELYDLAGEYVTIADISNNNNNNKNIGRRNTGMGIYYSNDIVSKRVEPSTSAVSEYVSLNELPQHLRRHAVSSRSAPAAESESCAQQQLPVEPAAATSQPPPLPLGGRTQGVGDGVPMYAEIGELSEEKPLASAANRRLRPNAEGKLVYDSDSLKRRKGAHTTFAPGPYVKETESALHSAESAAAAIASSQDPSAASGAISSTKSAKLLLLTSGGLISGNRKVANVRPIVAKSPLSRNVSEMATYQATSYQDQKQLLANRTNPFYETIAAPVANLMMPSPSGKSNSSTGSTATSSSSSASGQSGEEGKPERLPSASSRPSACLPTTKSQSESLSLSASGFPVNNTATTETSAKSTLNHHIATKSAGSISSVREHQPQHPNQNPKDGESKVRVVEMETTTLGDAFEMGEQQGPPLPPRAKVNDKQHPTKPLSIDPIALHFSIAGNPGQPRTALKRNNSYRLANDELKLEMNKENLSVSFGSERAPSSGAMPLSSVHQQHATSSLRLRNLSNHLPTNTHSPGGERRIAKSFERLVDEFSLSLTTGNDSSYLKNKSLENFDDSPALLSVASPEQRPDKNASNSSDSESEDDEEELAPPLSQSLPAPKYHKSFVDSVLLRPFKIILPSDENGNRRQRILQKFEVTEIW

Target ORF information:

RefSeq Version	NM_001104364.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster GUK-holder (gukh), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104364.2

ORF Insert Sequence:

ATGCCCTTCG TCCAGCGCGT GGTGCAGCCC GTCAATGTGG CCCAGGCCAC GGCGGCCAGT 
TTGGGCCACG CATCCGGACG ATTCCACTGC ACGCCGGGCA AGTGCCGGAA CAACAACTGC 
ATTAACAAGC AGTCGCCGGA TGTAGAGGTC CCCAATGGCC ACGGATCACC CACATCCCTT 
ACCAGGGTTC TGGTTCACCA GGCGCCGGAG GATCAACCCG TACCGCCCGC TCCGGCGCCC 
ATGAAGAAGC TAAAGCACCA CGTCGAGTTC CTGTCCACCT CGCCCACTCG CCATCAGTCG 
CAATCCCTGC CCAATTCGCG GCATCCCAGC CAGCAGAGGC CGGCGCTTGG AAGGATCGCC 
AGTGCACCGC AGTCGCCCAC TGCCGGCTCC TCCAAGGTGG TCTCCGGCCA CGAGTTCGAC 
TCGATCAGCA ACATAACGCT GAGCAACGCC CTGCGGCAGC TGGCCAGCTT GGTCCTCATA 
GCCAGCGATA TCTTCGACGA CCTGCAGCGG GATCTGCAGG CGGTGGGCGA GCGGGCGGGT 
CGTGTGCAGC GGAAGATTGC CGCCGTGGAG CGACGCGTCT GTGCCTACGA TCCGAAAACG 
GTCACAGTTC CTGAAAGCGA CCTGCTCACC TTCGCACAGC GCAAACACCA CTTCGAGACG 
GACAAGTCAT TCCAGCAGGA GCTCTTTACA GGCGAGACCA GACCTCTGAG CGTCCGGCAG 
CTTTACACGG AGGCTGGTAA GGAGCTGCCG GTGGCCACCG GAGCCACTGC GACTGCCCTC 
GCCTCCTTGG CGCACTCGCA GTCGCTGATC CCGTCCCGTT CCATCTCCTT CAACGGTGAG 
GTTCTGTCCA GCGATCACGA AGTTCTACAA CTAAATGGAT CCACGGACAC GGAGGATCAT 
CTGCTGTGCG TGTCGGACTT TGGCAATGCC AACCGCAAGC TGCGCACGCG CATCGATGCC 
GAGATCGAGA TCCGACTGCC CGCTGCCATT GAGGATTTGC GGAAGTGGAC GTCCAGCGAG 
GCGCTGGGTG ATGTCACCGT CACGCCGGAT TGCATGCATC ACGTGGACAC CTCGGTGAGC 
ACATCGCTGG CCATCGGCGA GAATGGCATC CTGACCCCGG CGCTGTCACC GTCCGTCCTA 
TCCGCCGATG CCGTCGATGC CCTCTACGCC CAGAATCTGA GCCAGGCGGC GGACAGCTCC 
CGGCACAACC ACCACACGCA AGCCCTCATC CCGAACGATA TTAATAAAGA CGTGCCTTTG 
AATCATCGAT TGCCTTCACC CGAAGAACAG ACCAAGCAGA TTGCCTTAAA ATACCCCGCC 
GAAGTGATTT CGGTGAACAC CAGCGGTAAG CACTTCCAGC GGATGTGTGC GGCTCGCAAG 
TCGACCAGTG GAGGATACGC AGGTGGCGCC ACCGCCGGAT CCTCGAGCAG CACATCGCCG 
GAGAACGCCG GCCAAACGGA GGGCAACAAC CTGGACGACA ATGTGCAGAC GGTGAGCCGG 
AGGTCAAGAT CGCGCAAGGT GCGCGGAAAG CGGCGGAACA CGATAGCCGG AATCGATCAG 
AAGGAGATCC AGGATGCGGC TAATGGCAAT GGGGAGTCCA AGAATGCCTC AAATGCCACA 
TCTCCGGTGG CTCCAGCTCA GCAGGCGGAG ACCACCTCGT CGGATGCCAA GAAGTCCAAG 
AAGTTCGGAC GCAGCAAGTC CAGTGACATA TTAAAAAAGG AATCTGCTGC CTTGCCCTAC 
GACAAGGGAA AGAGCACCCT CACCCGGCTG AATTCCCTGA AGCAGTGGGG TCGCAACCGC 
TTCAAGTTCA TGCATCGTAC CGGCGAACTG GGCGACCAGT TGGACACCAG CGGCTGCAGC 
TCCCAGAACA GTTCCGCGGA GAAGGCGGAC AGCCATCATG GTTCGCCGGC GGGCGACAAT 
TCCAAACGCG ACATCGACGA CGAATGCGTG GTGCATCATC TGGTGGCCCA GAAGAGTGAA 
TCTCGGTTTT CGCACGAACG AAAGCCCTCG TATTCCTCCT CGGAGAAGAG CATTAGTGTG 
TCCAGCAGTG GCCAGCTCAG GGGCAGACTG CAATTATCAT CCGGACCGAT GGCAGCGGCC 
CATGTAAAGA TGCGGGAAAC AGCCACTCTG AACAGGCAGC GACGAAACGG TCTACATGCA 
CTGGCTGGCA AGGAGGAGCA GCCGCATTCC TCAAGCGGCA ACTGGAGTGC CAGCTCGGAA 
TCGGGACGCG CCTCCATTGG CAGCGAGATC ACCATGCAGC CGAAGTCGAG TGCCTCGAGT 
ACATCGTTAA ATGTATCCAG TTTTCAGCCA GGAGGAGCGG GCAGTGGCCC ACCATCCTCC 
ATGCTGAGCA GAAGGAGATT CCTGAACACC TCCGCCTCCA GCAGTGTGAC GAGTGAGGGC 
ACTGCCACGC CGGAACTGCA GGCGGAGGCA GGTGCCTATC CAGGTCACCT AGATGATGAG 
ACAAGTTCCG CTTACAGCTG CGACACAGAG GGCTACTACA CATCCTTCCA CATGGACAGC 
GGTCTTCGAA CGCTGAAGGA AGAGGAGCCA TCCGTGATGC CCGGTACCCC GCTCCAGACG 
AGCCTGCACA CCAGCAGCTA CTCCTTCGGT AGCCAGTCCA ATCAAACAGT GCTCAATGCG 
GAGAGCGAGT ATGAGCTGTT CGGCAGAGGA TCCACCTCCA CGACAACGAG CTCCGCGGGC 
ACCGTGTGCA CAGCTCTGAT GAGCGGTGCC GCCTCTGGGC CGGATGTGCC CGAGCGGAGT 
AGCTCGCTGG GCAAGCACAG TGGTCAGAGC CGGAGCCAGA GCACCAGTGC CAGCAGCAGC 
ACGCTGGAGA GGAGCTACAG CAGCAGTACC ATCGGAAGCA CTCTGGAGCG CACGGGAACT 
ATCAAGAGGA ATGTCAAGAT GTCGCCAACC AAGGCAGGTG GTGAAGTGAC GGAGAAGGAT 
GACCAGCAGG ACCAGGATCA GCAACAGCAG CTTGAGTTCT CCGAGAGCTC CGATCTGGAG 
GGCGTGGAAC GCATAGAGCG GATCAAGCAG AAGACGGCCC TCACCTCCAA GAGAATACCC 
TCGATGTGCA TCATTACGCC CACAACGAGT GACGACGATG AGCACCAGAC CAATGATCCG 
AATCGCACGC TCACCAGTCT CACCATCGAT GTGGAGCACG GGGAGCAGGA CGAGGATGAG 
GCGGAGCAAA AGACGCCCAC GAATAGCAAG GACCACAACC TAAACGAACT GAAGCAGACG 
CCCACGAAAG CGCTGAGCGG GATGTTCGAG AAACTGAGGG TGAAACTGGT TCCGGGCAAA 
AGCTCTCCGG CCAAGAATCA AATGCAACCC AAGCCCGCCA ACTCGAGTGC CGTGAATGAC 
GATGAGCTTT ACGATTTGGC CGGCGAGTAC GTCACCATAG CAGACATCAG CAACAACAAC 
AACAACAACA AGAACATTGG GCGACGTAAC ACGGGAATGG GTATATACTA TTCCAATGAC 
ATAGTTTCCA AGAGGGTTGA ACCATCGACG TCTGCCGTTT CCGAGTACGT TTCGCTAAAC 
GAGCTGCCAC AGCACTTGCG TCGGCACGCG GTCTCGTCGA GATCCGCTCC TGCAGCGGAG 
AGCGAGTCCT GCGCACAACA ACAGCTGCCC GTCGAGCCGG CAGCTGCGAC GTCCCAACCA 
CCACCACTAC CCCTCGGCGG CAGGACGCAG GGGGTGGGCG ATGGGGTGCC CATGTACGCG 
GAAATCGGCG AATTGAGCGA AGAGAAGCCG CTCGCATCGG CGGCAAATCG TCGACTGCGC 
CCGAATGCCG AGGGGAAATT GGTCTACGAC TCCGACAGTC TGAAGCGGCG CAAGGGTGCA 
CACACCACCT TCGCACCTGG GCCGTACGTG AAGGAAACCG AAAGTGCGCT CCACAGTGCT 
GAAAGTGCAG CGGCGGCAAT AGCATCGTCA CAGGATCCAT CAGCAGCATC AGGAGCCATT 
AGCAGTACCA AGAGCGCCAA GCTCCTACTG CTCACCAGCG GCGGATTAAT CTCTGGCAAT 
CGCAAGGTGG CCAACGTGAG GCCCATTGTG GCCAAATCCC CGCTCAGCAG GAACGTCAGC 
GAAATGGCAA CCTACCAGGC AACCAGCTAC CAGGACCAGA AACAACTGCT GGCCAACCGA 
ACAAATCCCT TCTACGAGAC CATTGCCGCA CCCGTGGCCA ATCTGATGAT GCCCTCGCCG 
TCGGGCAAGT CCAATTCTTC GACGGGCTCC ACAGCCACCA GTTCCTCCTC ATCGGCATCG 
GGTCAATCCG GTGAAGAAGG TAAGCCAGAG CGGCTCCCGT CAGCCAGCAG CCGCCCCTCA 
GCTTGCTTAC CCACAACTAA ATCTCAATCC GAATCCCTAT CCTTATCTGC ATCCGGTTTC 
CCTGTAAATA ACACTGCCAC CACTGAAACG TCTGCCAAGA GCACCCTTAA TCACCACATA 
GCCACCAAGT CAGCGGGCAG CATATCAAGT GTAAGGGAGC ATCAGCCCCA GCATCCAAAT 
CAGAACCCCA AAGATGGCGA GTCGAAAGTG AGGGTTGTTG AAATGGAAAC TACCACCCTG 
GGGGATGCGT TCGAAATGGG GGAACAGCAA GGGCCACCCC TTCCGCCGAG GGCGAAAGTT 
AACGACAAAC AGCACCCCAC CAAGCCCCTA TCGATTGACC CAATTGCGCT GCACTTTTCC 
ATCGCTGGCA ATCCCGGCCA GCCGCGCACC GCACTCAAGC GGAACAACAG CTACCGGTTG 
GCCAACGATG AGCTCAAACT GGAGATGAAC AAGGAGAATC TCTCCGTGTC CTTCGGCTCC 
GAGAGAGCAC CTAGTTCGGG TGCAATGCCC CTAAGTTCCG TGCATCAGCA GCACGCAACA 
AGTTCCCTTA GGCTGCGCAA CCTGAGCAAC CACCTGCCCA CCAACACCCA CTCACCTGGC 
GGGGAGCGGC GGATAGCCAA GTCCTTTGAG CGCCTGGTGG ACGAGTTCTC GCTGTCGTTA 
ACCACGGGTA ACGATTCCTC CTATCTGAAG AACAAGTCGC TCGAGAACTT TGATGACTCA 
CCAGCCCTGT TGTCCGTAGC TTCGCCTGAA CAAAGGCCAG ACAAGAACGC CTCAAACTCC 
TCAGATTCCG AATCGGAGGA TGACGAGGAG GAGTTGGCCC CGCCTCTGTC CCAGTCCCTG 
CCAGCCCCAA AGTACCACAA GTCCTTTGTG GACAGTGTGC TGCTGCGCCC GTTCAAAATC 
ATCCTGCCCA GCGATGAGAA CGGCAACCGT CGCCAGCGCA TTCTACAGAA ATTCGAGGTT 
ACCGAGATAT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gukh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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