CG32354 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32354
Entrez Gene ID	50302
Full Name	CG32354 gene product from transcript CG32354-RA
Synonyms	BEST:CK01670,CG17355,CG7027,CG7159,CK01670,Dmel\CG32354
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66C11-66C12

mRNA and Protein(s)

mRNA	Protein	Name
NM_168275.2	NP_729380.1	uncharacterized protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG32354	NP_729380.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005450	XP_315453.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32354 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32354 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa20291	NM_168275.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG32354), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32354 ( NM_168275.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729380.1 Drosophila melanogaster uncharacterized protein (CG32354), mRNA.

Price & Availability↑

CloneID	OFa20291
Clone ID Related Accession (Same CDS sequence)	NM_168275.2
Accession Version	NM_168275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168275.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACCACA AACAGCATCT GCATCTGCAT CTGCAGCCCC CCGCCCCAAA CGCCACCCAA 
ACACAGGCCC ACGGACTTGG CCAGACTCCA AGCCAGATCG CCAGATGCAG CCATAACCAC 
AGCCCCAGTC TGGGCCTCAG CTTTGGCGGC ACCAAGAGCC AGCGACAGCT GTTTGGCGCA 
CTCTTGGCCA TGTTCACGTT GGCGGCATTT GCGTCAACCA CCAGTGGCGC TGTGACAGAC 
TCACCCATCA AGATTATCCG GCTGCCGGCG CAGGCGCCAG TTATCCGCTA TCGCAGTGCA 
GATGCGGCGC CCAACTCATT GCTGATCAAC TATCGTCAGG ATGCGCGACC GGAGCTGTCG 
TCCACATTGA TGCAGGCACC CGGAGCGGCG GCAGCTGCCT TGGAGTCGCT GCTGCGCGAG 
GAGACGGAGC AACAGCAGCT GTCACAGATG GGCAGACGGA ATAGGGCTAG TAACACCGGC 
ACCGGCAACT GTCCTCGATC CTGTCCACCG AGCATCACCG TGGGAGCTGA GCCCGTGTGC 
GGCAGCGATG GTCTGATCTA TGCGAATATC TGCGAGCTGC GCAAGAAGAC TTGCTCCCGC 
AGTGGAGTGT CGCTGATCAA GGACGTGCGG GATGGCTGCG AGAGGTCGAA GGGTTCGGAC 
TGCAAGCACC GCTGCAGCAC GGAGAAGGAT CCAGTGTGTG GTACAGACGG AAGGACCTAT 
CTGAATCGCT GCATGCTGAG GGTGCAATCG TGTCGCGTGG GATTGGCTGC AGTGAAGCTA 
TCGCATGTGG GTCCCTGCAG CAACACGAGT GCCGTGCGTG AGTCCTGTCC GGTTGACTGC 
AACAGTGCGC CGAAGGATGG ACCAGTTTGC TCATCGGATG GCAATGTATA CAACTCCACG 
TGCGAGATGA AGCTGAAGAC GTGTGGACAG GGTGTGGTGA AAACCAGCCG GAAGCACTGT 
CAGTCCACAC GAATGTGCAG GGAATCCTGC TGGCGTGTGG CGAGGCCCAC TTGCGGATCA 
GATGGCAGAC TGTATGCCAG TCCGTGCAAG ATGCGCTCTT CCAATTGCGG CAAGCACGTC 
TTTGAGGTTC CCTTATCCTA CTGCATGTCG CAGGAGAGGC ACGGAGCCTC CGATGCCTGT 
CCCACGGAGT GTCCCAAATC GGATACGGAT TCCTCGTCGC AGTACGTGTG CGGCAGTGAT 
GGCAACATAT ACTCTTCGCT GTGTGAACTC AAAATGCTCA ATTGCGGTCC ACAGCGCAAG 
TCCATTCAAA AAGTGAGCAT GGACAAGTGC AAGAATCGAC TGACCCGCTG CAAACAGCTG 
CCTCCTTGCA AGGACTTCAA TAGCTTGTTC GGTTCCATCT TCAGCTCGAA GCGAAATGAC 
AAGCTCTGCG GCACGGACGC CAAGACTTAT AACAATGAGT GCGAACTGGC CCACGCCACC 
TGCCTACGCG GCGTGAATCT GGCCCACATT GGTCCCTGCA CGGATCTCAA TTCGCCCACC 
AAGGATTGCG GGGATGCCTG TACTCGGGCG GATCTGGAAC AGCAGCCAGT GTGCGGCTCG 
GATGGCAACA CCTTCGCCTC CATGTGCGAG TTCAAGCGGC GCACTTGCGA TCTGCGTGTG 
GTGCCCGTTT CGCTAAAGAA TTGCGCTTTA ACCGCCGACT GCGAATCGGA TTGTGATGCC 
CAGCCGCCAA GTTTCGTCTG CGGATCGGAC AACAACTTGT ACAAGTCCGA GTGCCACATG 
CGCAAGGAGA ATTGTGGCAA GCATGTTTTC GTGGTGCCAC TGAAGCGCTG CCTTGCCGCC 
TTTCAGCTTA AAGGATGTGC CCGCATCTGT CCCAGGGAAT TCGAGCCTGT TTGCGGCAGC 
GACAATAAAA CGTATCTGAA CGACTGCTTC CTGGAGATCG AAAACTGCAG GGCGAACCAG 
ACGGTGAACG TCAACTACTA CGGCGCCTGT GGCCGCCCCG AAGCACCATC CACTAACTTC 
CTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729380.1
CDS	1140..3128
Translation	MHHKQHLHLHLQPPAPNATQTQAHGLGQTPSQIARCSHNHSPSLGLSFGGTKSQRQLFGALLAMFTLAAFASTTSGAVTDSPIKIIRLPAQAPVIRYRSADAAPNSLLINYRQDARPELSSTLMQAPGAAAAALESLLREETEQQQLSQMGRRNRASNTGTGNCPRSCPPSITVGAEPVCGSDGLIYANICELRKKTCSRSGVSLIKDVRDGCERSKGSDCKHRCSTEKDPVCGTDGRTYLNRCMLRVQSCRVGLAAVKLSHVGPCSNTSAVRESCPVDCNSAPKDGPVCSSDGNVYNSTCEMKLKTCGQGVVKTSRKHCQSTRMCRESCWRVARPTCGSDGRLYASPCKMRSSNCGKHVFEVPLSYCMSQERHGASDACPTECPKSDTDSSSQYVCGSDGNIYSSLCELKMLNCGPQRKSIQKVSMDKCKNRLTRCKQLPPCKDFNSLFGSIFSSKRNDKLCGTDAKTYNNECELAHATCLRGVNLAHIGPCTDLNSPTKDCGDACTRADLEQQPVCGSDGNTFASMCEFKRRTCDLRVVPVSLKNCALTADCESDCDAQPPSFVCGSDNNLYKSECHMRKENCGKHVFVVPLKRCLAAFQLKGCARICPREFEPVCGSDNKTYLNDCFLEIENCRANQTVNVNYYGACGRPEAPSTNFLY

Target ORF information:

RefSeq Version	NM_168275.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG32354), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168275.2

ORF Insert Sequence:

ATGCACCACA AACAGCATCT GCATCTGCAT CTGCAGCCCC CCGCCCCAAA CGCCACCCAA 
ACACAGGCCC ACGGACTTGG CCAGACTCCA AGCCAGATCG CCAGATGCAG CCATAACCAC 
AGCCCCAGTC TGGGCCTCAG CTTTGGCGGC ACCAAGAGCC AGCGACAGCT GTTTGGCGCA 
CTCTTGGCCA TGTTCACGTT GGCGGCATTT GCGTCAACCA CCAGTGGCGC TGTGACAGAC 
TCACCCATCA AGATTATCCG GCTGCCGGCG CAGGCGCCAG TTATCCGCTA TCGCAGTGCA 
GATGCGGCGC CCAACTCATT GCTGATCAAC TATCGTCAGG ATGCGCGACC GGAGCTGTCG 
TCCACATTGA TGCAGGCACC CGGAGCGGCG GCAGCTGCCT TGGAGTCGCT GCTGCGCGAG 
GAGACGGAGC AACAGCAGCT GTCACAGATG GGCAGACGGA ATAGGGCTAG TAACACCGGC 
ACCGGCAACT GTCCTCGATC CTGTCCACCG AGCATCACCG TGGGAGCTGA GCCCGTGTGC 
GGCAGCGATG GTCTGATCTA TGCGAATATC TGCGAGCTGC GCAAGAAGAC TTGCTCCCGC 
AGTGGAGTGT CGCTGATCAA GGACGTGCGG GATGGCTGCG AGAGGTCGAA GGGTTCGGAC 
TGCAAGCACC GCTGCAGCAC GGAGAAGGAT CCAGTGTGTG GTACAGACGG AAGGACCTAT 
CTGAATCGCT GCATGCTGAG GGTGCAATCG TGTCGCGTGG GATTGGCTGC AGTGAAGCTA 
TCGCATGTGG GTCCCTGCAG CAACACGAGT GCCGTGCGTG AGTCCTGTCC GGTTGACTGC 
AACAGTGCGC CGAAGGATGG ACCAGTTTGC TCATCGGATG GCAATGTATA CAACTCCACG 
TGCGAGATGA AGCTGAAGAC GTGTGGACAG GGTGTGGTGA AAACCAGCCG GAAGCACTGT 
CAGTCCACAC GAATGTGCAG GGAATCCTGC TGGCGTGTGG CGAGGCCCAC TTGCGGATCA 
GATGGCAGAC TGTATGCCAG TCCGTGCAAG ATGCGCTCTT CCAATTGCGG CAAGCACGTC 
TTTGAGGTTC CCTTATCCTA CTGCATGTCG CAGGAGAGGC ACGGAGCCTC CGATGCCTGT 
CCCACGGAGT GTCCCAAATC GGATACGGAT TCCTCGTCGC AGTACGTGTG CGGCAGTGAT 
GGCAACATAT ACTCTTCGCT GTGTGAACTC AAAATGCTCA ATTGCGGTCC ACAGCGCAAG 
TCCATTCAAA AAGTGAGCAT GGACAAGTGC AAGAATCGAC TGACCCGCTG CAAACAGCTG 
CCTCCTTGCA AGGACTTCAA TAGCTTGTTC GGTTCCATCT TCAGCTCGAA GCGAAATGAC 
AAGCTCTGCG GCACGGACGC CAAGACTTAT AACAATGAGT GCGAACTGGC CCACGCCACC 
TGCCTACGCG GCGTGAATCT GGCCCACATT GGTCCCTGCA CGGATCTCAA TTCGCCCACC 
AAGGATTGCG GGGATGCCTG TACTCGGGCG GATCTGGAAC AGCAGCCAGT GTGCGGCTCG 
GATGGCAACA CCTTCGCCTC CATGTGCGAG TTCAAGCGGC GCACTTGCGA TCTGCGTGTG 
GTGCCCGTTT CGCTAAAGAA TTGCGCTTTA ACCGCCGACT GCGAATCGGA TTGTGATGCC 
CAGCCGCCAA GTTTCGTCTG CGGATCGGAC AACAACTTGT ACAAGTCCGA GTGCCACATG 
CGCAAGGAGA ATTGTGGCAA GCATGTTTTC GTGGTGCCAC TGAAGCGCTG CCTTGCCGCC 
TTTCAGCTTA AAGGATGTGC CCGCATCTGT CCCAGGGAAT TCGAGCCTGT TTGCGGCAGC 
GACAATAAAA CGTATCTGAA CGACTGCTTC CTGGAGATCG AAAACTGCAG GGCGAACCAG 
ACGGTGAACG TCAACTACTA CGGCGCCTGT GGCCGCCCCG AAGCACCATC CACTAACTTC 
CTTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32354 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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