Atg10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atg10
Entrez Gene ID	50253
Full Name	Autophagy-related 10
Synonyms	ATG10,CG12821,Dmel\CG12821
General protein information	Preferred Names Autophagy-related 10 Names CG12821 gene product from transcript CG12821-RD Atg10-PB Atg10-PD Autophagy-specific gene 10 CG12821-PB CG12821-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 43E18-43E18

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103745.3	NP_001097215.1	Autophagy-related 10, isoform B
NM_001103746.3	NP_001097216.2	Autophagy-related 10, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Atg10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atg10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11521	NM_001103745.3 Latest version!	Drosophila melanogaster Autophagy-related 10 (Atg10), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11521	NM_001103746.3 Latest version!	Drosophila melanogaster Autophagy-related 10 (Atg10), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atg10 ( NM_001103745.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097215.1 Drosophila melanogaster Autophagy-related 10 (Atg10), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11521
Clone ID Related Accession (Same CDS sequence)	NM_001103745.3 , NM_001103746.3
Accession Version	NM_001103745.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 10, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001103745.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGACT TGAGCTGGAA AGATTTTTTG AGCCAGGCCA AGCAGTTCCT CGAAATTTCG 
CAGAAGTTGG GTGATAATTG GATATTGGAA CAAAAGGACT CGAATGAACC AAACACCTAT 
CTCAAATGTA GTCAGAAGAT TAAATGCCGA GGTGGCAAGG ACAACTCGGC TGAGTTGGTT 
AGTGTGGAGT ACCATGTGGT CTTCAGTGTT TCCTATCAAG TACCAATGTT GTTCTTCCAG 
GCACACAGAT CAGATGGAAG TCTTTTGGAT GTGGAGGCCA CTTGGCGAAT GTTCATGCCC 
GAATCGAAGG CCAGCGATCT CCACCAGATA CTCACCCAGA TGGATCACCC GGTGCTTTTC 
AGACCCTTTA TGGCATTGCA TCCATGCCGC ACCGCCGAAG TGCTAAAGCA GTTCGGAAAG 
CCCAGCTGCA ACCAGGTGCT TACCTTCATC AGTCTGTACG GACCCCACGT GCAGTTGCAC 
CTGCAAAATG CGTACGGCTT AAGCCAAGAG TATACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097215.1
CDS	58..576
Translation	MGDLSWKDFLSQAKQFLEISQKLGDNWILEQKDSNEPNTYLKCSQKIKCRGGKDNSAELVSVEYHVVFSVSYQVPMLFFQAHRSDGSLLDVEATWRMFMPESKASDLHQILTQMDHPVLFRPFMALHPCRTAEVLKQFGKPSCNQVLTFISLYGPHVQLHLQNAYGLSQEYT

Target ORF information:

RefSeq Version	NM_001103745.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 10 (Atg10), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103745.3

ORF Insert Sequence:

ATGGGCGACT TGAGCTGGAA AGATTTTTTG AGCCAGGCCA AGCAGTTCCT CGAAATTTCG 
CAGAAGTTGG GTGATAATTG GATATTGGAA CAAAAGGACT CGAATGAACC AAACACCTAT 
CTCAAATGTA GTCAGAAGAT TAAATGCCGA GGTGGCAAGG ACAACTCGGC TGAGTTGGTT 
AGTGTGGAGT ACCATGTGGT CTTCAGTGTT TCCTATCAAG TACCAATGTT GTTCTTCCAG 
GCACACAGAT CAGATGGAAG TCTTTTGGAT GTGGAGGCCA CTTGGCGAAT GTTCATGCCC 
GAATCGAAGG CCAGCGATCT CCACCAGATA CTCACCCAGA TGGATCACCC GGTGCTTTTC 
AGACCCTTTA TGGCATTGCA TCCATGCCGC ACCGCCGAAG TGCTAAAGCA GTTCGGAAAG 
CCCAGCTGCA ACCAGGTGCT TACCTTCATC AGTCTGTACG GACCCCACGT GCAGTTGCAC 
CTGCAAAATG CGTACGGCTT AAGCCAAGAG TATACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atg10 ( NM_001103746.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097216.2 Drosophila melanogaster Autophagy-related 10 (Atg10), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11521
Clone ID Related Accession (Same CDS sequence)	NM_001103745.3 , NM_001103746.3
Accession Version	NM_001103746.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Autophagy-related 10, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103746.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGACT TGAGCTGGAA AGATTTTTTG AGCCAGGCCA AGCAGTTCCT CGAAATTTCG 
CAGAAGTTGG GTGATAATTG GATATTGGAA CAAAAGGACT CGAATGAACC AAACACCTAT 
CTCAAATGTA GTCAGAAGAT TAAATGCCGA GGTGGCAAGG ACAACTCGGC TGAGTTGGTT 
AGTGTGGAGT ACCATGTGGT CTTCAGTGTT TCCTATCAAG TACCAATGTT GTTCTTCCAG 
GCACACAGAT CAGATGGAAG TCTTTTGGAT GTGGAGGCCA CTTGGCGAAT GTTCATGCCC 
GAATCGAAGG CCAGCGATCT CCACCAGATA CTCACCCAGA TGGATCACCC GGTGCTTTTC 
AGACCCTTTA TGGCATTGCA TCCATGCCGC ACCGCCGAAG TGCTAAAGCA GTTCGGAAAG 
CCCAGCTGCA ACCAGGTGCT TACCTTCATC AGTCTGTACG GACCCCACGT GCAGTTGCAC 
CTGCAAAATG CGTACGGCTT AAGCCAAGAG TATACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097216.2
CDS	58..576
Translation	MGDLSWKDFLSQAKQFLEISQKLGDNWILEQKDSNEPNTYLKCSQKIKCRGGKDNSAELVSVEYHVVFSVSYQVPMLFFQAHRSDGSLLDVEATWRMFMPESKASDLHQILTQMDHPVLFRPFMALHPCRTAEVLKQFGKPSCNQVLTFISLYGPHVQLHLQNAYGLSQEYT

Target ORF information:

RefSeq Version	NM_001103746.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Autophagy-related 10 (Atg10), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103746.3

ORF Insert Sequence:

ATGGGCGACT TGAGCTGGAA AGATTTTTTG AGCCAGGCCA AGCAGTTCCT CGAAATTTCG 
CAGAAGTTGG GTGATAATTG GATATTGGAA CAAAAGGACT CGAATGAACC AAACACCTAT 
CTCAAATGTA GTCAGAAGAT TAAATGCCGA GGTGGCAAGG ACAACTCGGC TGAGTTGGTT 
AGTGTGGAGT ACCATGTGGT CTTCAGTGTT TCCTATCAAG TACCAATGTT GTTCTTCCAG 
GCACACAGAT CAGATGGAAG TCTTTTGGAT GTGGAGGCCA CTTGGCGAAT GTTCATGCCC 
GAATCGAAGG CCAGCGATCT CCACCAGATA CTCACCCAGA TGGATCACCC GGTGCTTTTC 
AGACCCTTTA TGGCATTGCA TCCATGCCGC ACCGCCGAAG TGCTAAAGCA GTTCGGAAAG 
CCCAGCTGCA ACCAGGTGCT TACCTTCATC AGTCTGTACG GACCCCACGT GCAGTTGCAC 
CTGCAAAATG CGTACGGCTT AAGCCAAGAG TATACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Atg10 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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