Fis1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fis1
Entrez Gene ID	49892
Full Name	Fission, mitochondrial 1
Synonyms	BcDNA:RE29957,CG17510,Dmel\CG17510,NEST:bs15a08,bs15a08.y1,dFis1
General protein information	Preferred Names Fission, mitochondrial 1 Names CG17510 gene product from transcript CG17510-RA CG17510-PA CG17510-PC CG17510-PD CG17510-PE CG17510-PF CG17510-PG Fis1-PA Fis1-PC Fis1-PD Fis1-PE Fis1-PF Fis1-PG
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41F5-41F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_143843.4	NP_652100.1	Fission, mitochondrial 1, isoform A
NM_001144135.2	NP_001137607.1	Fission, mitochondrial 1, isoform C
NM_001144136.3	NP_001137608.1	Fission, mitochondrial 1, isoform E
NM_001144137.3	NP_001137609.1	Fission, mitochondrial 1, isoform F
NM_165439.4	NP_724420.1	Fission, mitochondrial 1, isoform D
NM_001299211.1	NP_001286140.1	Fission, mitochondrial 1, isoform G

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FIS1	XP_001143081.2
Bos taurus (cattle)	FIS1	NP_001029956.1
Danio rerio (zebrafish)	im:6905231	XP_005172294.1
Caenorhabditis elegans (roundworm)	fis-2	NP_001024560.1
Xenopus tropicalis (tropical clawed frog)	fis1	NP_001123678.1
Canis lupus familiaris (dog)	FIS1	XP_536854.1
Homo sapiens (human)	FIS1	NP_057152.2
	FIS1	XP_001108062.1
Mus musculus (house mouse)	Fis1	NP_079838.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009852	XP_318964.4
Drosophila melanogaster (fruit fly)	Fis1	NP_001137607.1
Rattus norvegicus (Norway rat)	Fis1	NP_001099389.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fis1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fis1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11487	NM_143843.4 Latest version!	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11487	NM_001299211.1 Latest version!	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11488	NM_001144135.2 Latest version!	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11489	NM_001144137.3 Latest version!	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11489	NM_165439.4 Latest version!	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa11490	NM_001144136.3 Latest version!	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fis1 ( NM_143843.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652100.1 Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11487
Clone ID Related Accession (Same CDS sequence)	NM_143843.4 , NM_001299211.1
Accession Version	NM_143843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fission, mitochondrial 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_143843.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAATAAAC AAAAACGGGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652100.1
CDS	181..477
Translation	MILEELARTHPDGRRDYIYYLAFGNARIKEYTSGLKYCRAFLDIESNDQVRSLEEYIKKEIDKEVAKGMVVAGGAALVLGGILGLGIAMARNKQKREK

Target ORF information:

RefSeq Version	NM_143843.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143843.4

ORF Insert Sequence:

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAATAAAC AAAAACGGGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_001299211.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286140.1 Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa11487
Clone ID Related Accession (Same CDS sequence)	NM_143843.4 , NM_001299211.1
Accession Version	NM_001299211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fission, mitochondrial 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAATAAAC AAAAACGGGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286140.1
CDS	108..404
Translation	MILEELARTHPDGRRDYIYYLAFGNARIKEYTSGLKYCRAFLDIESNDQVRSLEEYIKKEIDKEVAKGMVVAGGAALVLGGILGLGIAMARNKQKREK

Target ORF information:

RefSeq Version	NM_001299211.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299211.1

ORF Insert Sequence:

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAATAAAC AAAAACGGGA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_001144135.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137607.1 Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11488
Clone ID Related Accession (Same CDS sequence)	NM_001144135.2
Accession Version	NM_001144135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fission, mitochondrial 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144135.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGATC TTTTAAACGA AGTTGTACCA CAAGAAGATT TAGAAAGATT CGAAAAAAAG 
TATCATCACG AACTTGAGTT GGATGGTGAA GTTACCACAG ACACAAAGTT TGAGTACGCA 
TTCTGTCTGG TCCGAAGTCG GTACACAAAT GACGTTAGAA AGGGAATTAT GATTTTGGAG 
GAACTGGCTC GTACGCATCC AGATGGAAGG CGTGACTATA TATATTATCT TGCGTTTGGT 
AATGCTCGTA TCAAGGAGTA TACGTCTGGC TTAAAATACT GCCGAGCTTT TCTTGACATC 
GAGTCAAATG ATCAAGTTCG CTCCCTAGAG GAATATATTA AAAAAGAAAT CGATAAGGAA 
GTGGCAAAGG GTATGGTGGT TGCAGGCGGA GCAGCTTTAG TACTTGGCGG AATATTAGGA 
CTTGGCATTG CTATGGCTAG AAATAAACAA AAACGGGAGA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137607.1
CDS	81..545
Translation	MEDLLNEVVPQEDLERFEKKYHHELELDGEVTTDTKFEYAFCLVRSRYTNDVRKGIMILEELARTHPDGRRDYIYYLAFGNARIKEYTSGLKYCRAFLDIESNDQVRSLEEYIKKEIDKEVAKGMVVAGGAALVLGGILGLGIAMARNKQKREK

Target ORF information:

RefSeq Version	NM_001144135.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144135.2

ORF Insert Sequence:

ATGGAGGATC TTTTAAACGA AGTTGTACCA CAAGAAGATT TAGAAAGATT CGAAAAAAAG 
TATCATCACG AACTTGAGTT GGATGGTGAA GTTACCACAG ACACAAAGTT TGAGTACGCA 
TTCTGTCTGG TCCGAAGTCG GTACACAAAT GACGTTAGAA AGGGAATTAT GATTTTGGAG 
GAACTGGCTC GTACGCATCC AGATGGAAGG CGTGACTATA TATATTATCT TGCGTTTGGT 
AATGCTCGTA TCAAGGAGTA TACGTCTGGC TTAAAATACT GCCGAGCTTT TCTTGACATC 
GAGTCAAATG ATCAAGTTCG CTCCCTAGAG GAATATATTA AAAAAGAAAT CGATAAGGAA 
GTGGCAAAGG GTATGGTGGT TGCAGGCGGA GCAGCTTTAG TACTTGGCGG AATATTAGGA 
CTTGGCATTG CTATGGCTAG AAATAAACAA AAACGGGAGA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_001144137.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137609.1 Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa11489
Clone ID Related Accession (Same CDS sequence)	NM_001144137.3 , NM_165439.4
Accession Version	NM_001144137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fission, mitochondrial 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144137.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137609.1
CDS	325..603
Translation	MILEELARTHPDGRRDYIYYLAFGNARIKEYTSGLKYCRAFLDIESNDQVRSLEEYIKKEIDKEVAKGMVVAGGAALVLGGILGLGIAMARK

Target ORF information:

RefSeq Version	NM_001144137.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144137.3

ORF Insert Sequence:

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_165439.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724420.1 Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11489
Clone ID Related Accession (Same CDS sequence)	NM_001144137.3 , NM_165439.4
Accession Version	NM_165439.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fission, mitochondrial 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165439.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724420.1
CDS	181..459
Translation	MILEELARTHPDGRRDYIYYLAFGNARIKEYTSGLKYCRAFLDIESNDQVRSLEEYIKKEIDKEVAKGMVVAGGAALVLGGILGLGIAMARK

Target ORF information:

RefSeq Version	NM_165439.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165439.4

ORF Insert Sequence:

ATGATTTTGG AGGAACTGGC TCGTACGCAT CCAGATGGAA GGCGTGACTA TATATATTAT 
CTTGCGTTTG GTAATGCTCG TATCAAGGAG TATACGTCTG GCTTAAAATA CTGCCGAGCT 
TTTCTTGACA TCGAGTCAAA TGATCAAGTT CGCTCCCTAG AGGAATATAT TAAAAAAGAA 
ATCGATAAGG AAGTGGCAAA GGGTATGGTG GTTGCAGGCG GAGCAGCTTT AGTACTTGGC 
GGAATATTAG GACTTGGCAT TGCTATGGCT AGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fis1 ( NM_001144136.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137608.1 Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa11490
Clone ID Related Accession (Same CDS sequence)	NM_001144136.3
Accession Version	NM_001144136.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Fission, mitochondrial 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001144136.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGATC TTTTAAACGA AGTTGTACCA CAAGAAGATT TAGAAAGATT CGAAAAAAAG 
TATCATCACG AACTTGAGTT GGATGGTGAA GTTACCACAG ACACAAAGTT TGAGTACGCA 
TTCTGTCTGG TCCGAAGTCG GTACACAAAT GACGTTAGAA AGGGAATTAT GATTTTGGAG 
GAACTGGCTC GTACGCATCC AGATGGAAGG CGTGACTATA TATATTATCT TGCGTTTGGT 
AATGCTCGTA TCAAGGAGTA TACGTCTGGC TTAAAATACT GCCGAGCTTT TCTTGACATC 
GAGTCAAATG ATCAAGTTCG CTCCCTAGAG GAATATATTA AAAAAGAAAT CGATAAGGAA 
GTGGCAAAGG GTATGGTGGT TGCAGGCGGA GCAGCTTTAG TACTTGGCGG AATATTAGGA 
CTTGGCATTG CTATGGCTAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137608.1
CDS	81..527
Translation	MEDLLNEVVPQEDLERFEKKYHHELELDGEVTTDTKFEYAFCLVRSRYTNDVRKGIMILEELARTHPDGRRDYIYYLAFGNARIKEYTSGLKYCRAFLDIESNDQVRSLEEYIKKEIDKEVAKGMVVAGGAALVLGGILGLGIAMARK

Target ORF information:

RefSeq Version	NM_001144136.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Fission, mitochondrial 1 (Fis1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144136.3

ORF Insert Sequence:

ATGGAGGATC TTTTAAACGA AGTTGTACCA CAAGAAGATT TAGAAAGATT CGAAAAAAAG 
TATCATCACG AACTTGAGTT GGATGGTGAA GTTACCACAG ACACAAAGTT TGAGTACGCA 
TTCTGTCTGG TCCGAAGTCG GTACACAAAT GACGTTAGAA AGGGAATTAT GATTTTGGAG 
GAACTGGCTC GTACGCATCC AGATGGAAGG CGTGACTATA TATATTATCT TGCGTTTGGT 
AATGCTCGTA TCAAGGAGTA TACGTCTGGC TTAAAATACT GCCGAGCTTT TCTTGACATC 
GAGTCAAATG ATCAAGTTCG CTCCCTAGAG GAATATATTA AAAAAGAAAT CGATAAGGAA 
GTGGCAAAGG GTATGGTGGT TGCAGGCGGA GCAGCTTTAG TACTTGGCGG AATATTAGGA 
CTTGGCATTG CTATGGCTAG AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Fis1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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