Pp1-87B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pp1-87B
Entrez Gene ID	49260
Full Name	Protein phosphatase 1 at 87B
Synonyms	87B,CG5650,DmPp1-87B,Dmel\CG5650,PP-1alpha,PP1,PP1 87B,PP1 beta87b,PP1-87B,PP187B,PP1A87B,PP1B,PP1alpha-87B,PP1alpha87B,PP1alpha[[2]],PP1c,PP1c 87B,PPI 87B,Pp-1alpha,Pp1,Pp1-87Bb,Pp1alpha-87B,Ppl-87B,Su(Var)3-6,Su(var)(3)6,Su(var)3-6,Su-var(3)6,Suvar(3)6,ck19,dPP1beta,l(3)87Bg,l(3)ck19,l(3)j6E7,pp1-87B,su-var(3)6
General protein information	Preferred Names Protein phosphatase 1 at 87B Names CG5650 gene product from transcript CG5650-RA CG5650-PA Pp1-87B-PA protein phosphatase 1 protein phosphatase 1 at 87B suppressor of variegation
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87B9-87B10

mRNA and Protein(s)

mRNA	Protein	Name
NM_080198.3	NP_524937.1	protein phosphatase 1 at 87B

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1CC	NP_002701.1
Bos taurus (cattle)	PPP1CC	NP_777006.1
Mus musculus (house mouse)	Gm5601	XP_006508478.1
Oryza sativa (rice)	Os03g0268000	NP_001049669.1
Xenopus tropicalis (tropical clawed frog)	ppp1cc	NP_998835.1
Canis lupus familiaris (dog)	PPP1CC	NP_001003033.1
Drosophila melanogaster (fruit fly)	Pp1alpha-96A	NP_001262919.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011166	XP_309483.4
	PPP1CC	XP_001108424.1
Danio rerio (zebrafish)	ppp1cc	XP_700468.5
Gallus gallus (chicken)	PPP1CC	NP_001006190.1
Schizosaccharomyces pombe (fission yeast)	sds21	NP_587898.1
Pan troglodytes (chimpanzee)	PPP1CC	XP_001144540.2
Drosophila melanogaster (fruit fly)	Pp1-87B	NP_524937.1
Drosophila melanogaster (fruit fly)	Pp1-13C	NP_524921.1
Arabidopsis thaliana (thale cress)	TOPP4	NP_181514.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ectopic expression of dRif1 causes enlarged larval imaginal discs and early pupal lethality which is completely reversed by co-expression of PP1 87B, the major protein phosphatase in Drosophila, indicating genetic and functional interaction
Title: Drosophila Rif1 is an essential gene and controls late developmental events by direct interaction with PP1-87B.

these findings shed light on the in vivo role of the PNUTS-PP1 holoenzyme and its contribution to the control of gene expression during early Drosophila development.
Title: PNUTS/PP1 regulates RNAPII-mediated gene expression and is necessary for developmental growth.

PP1-87B has a role in coupling moesin-dependent cell shape changes to mitotic exit.
Title: PP1-mediated moesin dephosphorylation couples polar relaxation to mitotic exit.

PP1 is not a major regulator of the Rbf1/E2F1 pathway in Drosophila
Title: Normal regulation of Rbf1/E2f1 target genes in Drosophila type 1 protein phosphatase mutants.

Bif regulates PP1 activity, and both proteins influence cytoskeleton dynamics in the growth cone of R cells to allow proper axon targeting.
Title: Bifocal and PP1 interaction regulates targeting of the R-cell growth cone in Drosophila.

The following Pp1-87B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pp1-87B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06972	NM_080198.3 Latest version!	Drosophila melanogaster protein phosphatase 1 at 87B (Pp1-87B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pp1-87B ( NM_080198.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524937.1 Drosophila melanogaster protein phosphatase 1 at 87B (Pp1-87B), mRNA.

Price & Availability↑

CloneID	OFa06972
Clone ID Related Accession (Same CDS sequence)	NM_080198.3
Accession Version	NM_080198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 1 at 87B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_080198.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCGACG TGATGAATAT CGACAGCATA ATATCGCGAC TTCTCGAGGT GCGTGGGGCA 
CGGCCAGGTA AAAACGTACA GCTCTCGGAG GGCGAGATCC GGGGACTTTG CTTGAAGTCG 
CGCGAGATCT TCCTGTCGCA GCCCATTCTG CTCGAGCTAG AGGCACCGTT GAAGATCTGC 
GGCGACATCC ATGGACAGTA CTACGATCTG TTGCGTCTGT TCGAGTACGG CGGCTTTCCG 
CCGGAATCGA ATTACCTGTT CCTCGGCGAC TACGTCGATC GCGGCAAGCA ATCGCTGGAG 
ACGATCTGCC TGCTGCTCGC CTACAAGATC AAATACTCGG AGAATTTCTT CCTGCTGCGC 
GGCAACCACG AATGCGCCAG CATTAATCGC ATATACGGAT TCTACGACGA ATGCAAGCGT 
CGCTACAGCA TCAAGTTGTG GAAGACATTC ACGGACTGCT TCAACTGCCT GCCAGTGGCG 
GCCATTGTCG ACGAGAAGAT CTTCTGCTGC CACGGTGGTC TCAGTCCCGA TTTGACCTCC 
ATGGAGCAGA TCCGTCGCAT TATGCGGCCA ACCGATGTGC CCGACCAGGG ACTGCTGTGC 
GATCTCCTGT GGTCCGATCC CGATAAGGAT ACCATGGGCT GGGGCGAAAA CGACCGCGGC 
GTTAGCTTCA CCTTCGGTGC CGAGGTGGTG GCCAAGTTTT TGCAGAAGCA CGAGTTCGAT 
CTCATCTGCC GAGCCCATCA AGTCGTCGAG GATGGGTACG AGTTCTTTGC CAAACGCATG 
CTGGTCACCC TGTTCTCGGC GCCCAACTAC TGCGGCGAGT TCGACAATGC CGGCGCCATG 
ATGTCCGTGG ACGATACGCT GATGTGCTCG TTCCAAATCC TCAAGCCAGC CGACAAGCGT 
AAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524937.1
CDS	230..1138
Translation	MGDVMNIDSIISRLLEVRGARPGKNVQLSEGEIRGLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYSENFFLLRGNHECASINRIYGFYDECKRRYSIKLWKTFTDCFNCLPVAAIVDEKIFCCHGGLSPDLTSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDTMGWGENDRGVSFTFGAEVVAKFLQKHEFDLICRAHQVVEDGYEFFAKRMLVTLFSAPNYCGEFDNAGAMMSVDDTLMCSFQILKPADKRKK

Target ORF information:

RefSeq Version	NM_080198.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 1 at 87B (Pp1-87B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080198.3

ORF Insert Sequence:

ATGGGCGACG TGATGAATAT CGACAGCATA ATATCGCGAC TTCTCGAGGT GCGTGGGGCA 
CGGCCAGGTA AAAACGTACA GCTCTCGGAG GGCGAGATCC GGGGACTTTG CTTGAAGTCG 
CGCGAGATCT TCCTGTCGCA GCCCATTCTG CTCGAGCTAG AGGCACCGTT GAAGATCTGC 
GGCGACATCC ATGGACAGTA CTACGATCTG TTGCGTCTGT TCGAGTACGG CGGCTTTCCG 
CCGGAATCGA ATTACCTGTT CCTCGGCGAC TACGTCGATC GCGGCAAGCA ATCGCTGGAG 
ACGATCTGCC TGCTGCTCGC CTACAAGATC AAATACTCGG AGAATTTCTT CCTGCTGCGC 
GGCAACCACG AATGCGCCAG CATTAATCGC ATATACGGAT TCTACGACGA ATGCAAGCGT 
CGCTACAGCA TCAAGTTGTG GAAGACATTC ACGGACTGCT TCAACTGCCT GCCAGTGGCG 
GCCATTGTCG ACGAGAAGAT CTTCTGCTGC CACGGTGGTC TCAGTCCCGA TTTGACCTCC 
ATGGAGCAGA TCCGTCGCAT TATGCGGCCA ACCGATGTGC CCGACCAGGG ACTGCTGTGC 
GATCTCCTGT GGTCCGATCC CGATAAGGAT ACCATGGGCT GGGGCGAAAA CGACCGCGGC 
GTTAGCTTCA CCTTCGGTGC CGAGGTGGTG GCCAAGTTTT TGCAGAAGCA CGAGTTCGAT 
CTCATCTGCC GAGCCCATCA AGTCGTCGAG GATGGGTACG AGTTCTTTGC CAAACGCATG 
CTGGTCACCC TGTTCTCGGC GCCCAACTAC TGCGGCGAGT TCGACAATGC CGGCGCCATG 
ATGTCCGTGG ACGATACGCT GATGTGCTCG TTCCAAATCC TCAAGCCAGC CGACAAGCGT 
AAAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pp1-87B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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