Tor cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tor
Entrez Gene ID	47396
Full Name	Target of rapamycin
Synonyms	5092,CG5092,CT16317,CT24745,CT24817,DmTOR,Dmel\CG5092,FRAP/TOR,TOR,TORC1,Torc1,Tor,TOR,dTOR,dTor,dtor,l(2)k17004,mTOR,mTor,tor
General protein information	Preferred Names Target of rapamycin Names CG5092 gene product from transcript CG5092-RB CG5092-PA CG5092-PB Target-of-rapamycin Tor-PA Tor-PB target of rapamycin target of rapamycin complex 1 target of rapamycin kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 34A4-34A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_080152.3	NP_524891.1	target of rapamycin, isoform A
NM_001273498.1	NP_001260427.1	target of rapamycin, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	MTOR	NP_004949.1
Canis lupus familiaris (dog)	MTOR	XP_005618060.1
Gallus gallus (chicken)	MTOR	XP_417614.3
Danio rerio (zebrafish)	mtor	NP_001070679.2
Schizosaccharomyces pombe (fission yeast)	tor2	NP_595359.2
Kluyveromyces lactis	KLLA0B13948g	XP_452152.1
Neurospora crassa	NCU05608	XP_960570.1
Pan troglodytes (chimpanzee)	MTOR	XP_003949341.1
Bos taurus (cattle)	MTOR	XP_005217047.1
Drosophila melanogaster (fruit fly)	Tor	NP_001260427.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007873	XP_317619.4
Arabidopsis thaliana (thale cress)	TOR	NP_175425.2
Xenopus tropicalis (tropical clawed frog)	mtor	XP_004916088.1
Rattus norvegicus (Norway rat)	Mtor	NP_063971.1
Oryza sativa (rice)	Os05g0235300	NP_001054988.2
Mus musculus (house mouse)	Mtor	NP_064393.2
Saccharomyces cerevisiae (baker's yeast)	TOR2	NP_012719.2
Eremothecium gossypii	AGOS_AFR420W	NP_985967.2
Magnaporthe oryzae (rice blast fungus)	MGG_15156	XP_003710485.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TORC1 is selectively activated in the second mitotic wave of the developing Drosophila eye. TORC1 is regulated by Hedgehog and E2F1 pathway.
Title: Spatial Activation of TORC1 Is Regulated by Hedgehog and E2F1 Signaling in the Drosophila Eye.

TORC1 activity in the wing disc is patchy. TORC1 activity is high in cells at the G1/S transition due to CycD/Cdk4.
Title: CycD/Cdk4 and Discontinuities in Dpp Signaling Activate TORC1 in the Drosophila Wing Disc.

To characterize natural genetic variation in the IIS/TOR pathway, we used >250 recombinant inbred lines (RILs) derived from a multiparental mapping population, the Drosophila Synthetic Population Resource, to map transcript-level QTL of genes encoding 52 core IIS/TOR components in three different nutritional environments
Title: Genetic Dissection of Nutrition-Induced Plasticity in Insulin/Insulin-Like Growth Factor Signaling and Median Life Span in a

Studies show that the TOR signaling pathway and ATG5 and ATG7 are crucial for the regulation of cellular processes and can play various functions in different cell types in the brain.
Title: TOR signaling pathway and autophagy are involved in the regulation of circadian rhythms in behavior and plasticity of L2 interneurons in the brain of Drosophila melanogaster.

We show in vivo that autophagy is impaired in dGBA-deficient fly brains. In response, mechanistic target of rapamycin (mTOR) activity is downregulated in dGBA-deficient flies and rapamycin ameliorates the lifespan, locomotor, and oxidative stress phenotypes. dGBA knock-out flies also display an upregulation of the Drosophila ortholog of mammalian TFEB, Mitf, a response that is unable to overcome the autophagy block. Toget
Title: A Drosophila Model of Neuronopathic Gaucher Disease Demonstrates Lysosomal-Autophagic Defects and Altered mTOR Signalling and Is Functionally Rescued by Rapamycin.

The following Tor gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tor cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15249	NM_080152.3 Latest version!	Drosophila melanogaster target of rapamycin, transcript variant A (Tor), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa16861	NM_001273498.1 Latest version!	Drosophila melanogaster target of rapamycin, transcript variant B (Tor), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tor ( NM_080152.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524891.1 Drosophila melanogaster target of rapamycin, transcript variant A (Tor), mRNA.

Price & Availability↑

CloneID	OFa15249
Clone ID Related Accession (Same CDS sequence)	NM_080152.3
Accession Version	NM_080152.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of rapamycin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_080152.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACGA CGTCGGTGGT GCAGCAGTTC GTCAACGGGC TTAAGTCCCG CAACCGGAAT 
GTACAGAACA AGGCAACCCA GGATCTGCTC TTCTATGTGA AGACCGAGCT ACGCGAAATG 
TCCCAGGAGG AGCTGGCCCA ATTTTTCGAC GAGTTCGACC ATCACATTTT CACGATGGTG 
AATGCGACAG ATATCAACGA GAAAAAAGGC GGTGCCCTGG CCATGAAGTG CCTGATTAAC 
TGCGAGGGCA GTCTTACTGC CAGGAAGGGC ATTTCGCCCT ATCTCAACCG CCTGCGCGAT 
CTTCTGCTAA TCAACGATGT GTCCGTCATG GAGATTGCCG CCCGCTCCCT GGTCAAATTG 
GCCAACATGC CCACCTCCAA GGGCGCCGAT TCCTTCGACT TTGATATTAA GAAGGCTTTT 
GAGGTGCTCA GAGGCGAGAG ACAAGAGTAC CGCCGCCATT CAGCCGTCTT TATCCTCCGT 
GAGCTGGCTA TCGCCTTGCC CACATACTTC TACCAGCACA TCCTGACCTT CTTCGAGGTT 
ATCTTTAACG CCATCTTCGA TCCCAAACCT GCTATACGAG AGTCAGCTGG CGAGGCTTTG 
AGGGCGGCTT TGATTGTCAC CGCTCAGCGA GAGAGCACCA AGCAGTCCAG TGAACCACAG 
TGGTACAGAA TCTGCTATGA CGAGGCGAAT GGAAGCTTCA ATGCAGACTT GGGCTCTAGC 
AAGGATCAGA AAGGCGTGAC CCGAGACGAT CGCATTCACG GTGGCCTCGT CGTCTTCAAC 
GAGCTCTTTC GCTGTGCCAA TGCCACCTGG GAGCGGCGCT ACACGTCTCT GAAGACGCTG 
TTCCCCAAGA CCCAACACAA TAAGTTCCTG GAGGCCAGCA GTTCGTCGAG TATGGGTTCC 
CAGCTGAATA CTCTAGTGCC GCGGCTAAAG GTGCCCTTCA TTGACAAGCT CGGCAGCACC 
CAAACGCATT TGGGTGAGGG AGAGCATCAC AAAGGAGTTG CCAAGTTCGC GAGCCACAAC 
GTACTGGAGT CCGCATATGC TCAGGAGATC CTTCAGGAGC ACTACACCTC CATTTGCGAC 
AATGTTTTGG AGCAGCGAAC TTCGAAGTCT CCGTATGTAC AGCAGGCACT CCTTCAAATA 
CTTCCTCGAT TGGCTGCCTT TAACCGCGCG GTGTTTGTGG AAAAATACCT GCAGACATGC 
GTGTCGCATT TAATGCAGAT CCTGCGAGGC AAGGAGAAGG ATCGCACGGT GGCGTACATA 
ACCATTGGTT ACATGGCTGT AGCGGTGCAG AGTGCGATCG AAGTTCATTT ATCCAGTATA 
ATGACTAGCG TGAAGGTCGC CTTGCCGTCA AAGGATTTGA CCAGCAAACG AAAGGTACCA 
GTGGATCCTG CGGTCTTTGC CTGCATAACG CTTCTTGCTC ATGCCGTTAA GTCGGAGATC 
GCCGACGATG TTAAGGATAT CCTTGAGCAG ATGTTCTACA CGGGTCTCTC GCCAGCTCTG 
ACTGTTTGCC TGAGGGAACT CTCCGAGAAT GTGCCGCAGC TTAAGTCGGC CATTACAGAA 
GGATTGATCG GAATCCTATC CCAGGTGCTG ATGAACAAGG CGGCAATTTT ACCATACACT 
GCTTTGCCAA CGATCGCCAT CGATGGCTCG CTAATGCAGA ATGGTGACGG AGCAACCACA 
GTGCTGGCAC TCAAAACGCT GGGTACCTTC AACTTTGAGG AGCAAAACAT GCTGGACTTT 
GTGCAACGAT GTGCTGACTA CTTCATCGTT CACGAGCAGC AGGAGATTCG TCTGGAGGCA 
GTGCAAACAT GCACACGACT CCTTAAACTG GCAGTGCAAT CCTCTGAAAG TATGGAGAAC 
TCCAAGACCC TCAGCGACAC CGTTTCCCAC GTGATTGAGC GCCTGCTTAT GGTAGCCATT 
ACTGATATGG ACTGTAATGT GCGAATACGA ATTCTACGTT CGCTGGACGA GACCTTTGAT 
GGGAAGCTTG CTCAGCCAGA GTCTCTAAAT TCGCTATTTA TTACGCTGCA CGATGAAATC 
TTTGAGATCC GGGAGCTGGC AATGGTTACC ATTGGAAGAT TGTCGTCCAT AAACCCGGCT 
TATGTGATGC CCAAGCTAAG AACCACCATG ATCGAATTGA TCACGGATCT AAAGTACTCG 
GGAATGAGTC GAAACAAGGA GCAGAGCGCA AAGATGCTTG ACCATTTGGT GATAAGCACG 
CCCAGGCTAA TATCCTCTTA CATGAATCCG ATCCTCAAAG CCTTAGTGCC CAAGTTGCAT 
GAGCCGGAAT CGAATCCAGG AGTTATTTTA AATGTGCTTC GCACAATTGG TGATTTAGCA 
GAGGTTAATG GAGGCTCCGA TGAAATGGAA TTGTGGGCAG ATGACCTTCT GTCCATTTTA 
CTCGAAATGC TGGGCGACGC TGGCAGTCCG GATAAGCGAG GAGTGGCTCT GTGGACGTTG 
GGACAGTTAA TCAGTGCAAC AGGCAGGGTG GTTACCCCAT ATCACAAGTA CCCCGTGCTC 
ATAGATATTT TGATTAATTT CCTTAAAACG GAACAACGTC GATCCATTCG AAGGGAAACG 
ATACGAGTGC TGGGCTTGTT GGGAGCCATG GATCCTTACA AGCACAAGAT GAACAAGGGC 
TTGATTGATA GCCAAAAGGA TAATGTTTTA ATAGCATACT CCGATGGCAA AGTCGATGAA 
AGTCAGGACA TCTCCACCGC TGAGTTGCTA GTTAACATGG GTAACGCATT GGATGAGTAT 
TATCCCGCTG TGGCCATAGC TGCATTGATG AGGATTCTAC GGGATCCCAC GTTAAGTACA 
CGTCACACCA GCGTGGTGCA GGCGGTTACT TTTATCTTCC AGAGCCTGGG CATCAAATGT 
GTGCCGTACT TGGCCCAAGT GCTTCCCAAT TTGCTGGACA ATGTACGCAC TGCAGATAAT 
AATCTAAGGG AGTTTCTCTT CCAGCAGCTC GCCATTCTGG TGGCTTTTGT CAAGCTGCAT 
ATCATCAGCT ACATGGGAGA TATTTTTAAG CTGATCAAAG AATTTTGGAC CATTAATACG 
CCTCTGCAAA ACACTCTAAT CAATTTGATT GAACAGATCG CCGTGGCTTT GGGATGCGAG 
TTTAGGGATT ATTTGGCGGA GCTGATCCCA CAAATTCTGC GAGTGTTGCA GCACGATAAC 
TCCAAGGATA GAATGGTCAC GAGAAGGTTG CTCCAAGCTC TTCAAAAGTT TGGCAGCACT 
TTGGGTTACT ATCTGCCGCT GATTTTGCCT CCGATCGTGA AACTCTTCGA TTCTCCGTAC 
GTACCACAAC AGGTGTCAAT GGTAGCCCTG GAAACGATCA ACAATCTAGC CTGTCAGCTG 
GACTTCACCG ACTTCTCCTC GCGCATCATA CATCCGTTGG TTCGAGTGCT GGACGCTGAG 
CCAGAGCTTC GGGACCAAGC TATGACAACC CTTCGCTCAC TGGCAAAGCA ACTGGGCAAG 
AAGTACCTCG TGTTCGTGCC TATGGTCCAG CGCACTCTCA ACAAACATCG CATTGTGGAT 
CCCGAGTACG AGGAGCTACT GAGTAAGATT AAGTCCTGCA GCACACTGGC GGATTCCTAT 
GGGGCCGGGG AGTCAGAACT GCGGCCGTCC AGGTTCAAAA ACAACGAGCC ATTTGTGACC 
GACAGAAATA GCAATAATAA AAATCTGCAG GTCACCACAA ACGAACTACG AACGGCGTGG 
CAGGTGACGC GGCGGGTGTC CAAGGACGAC TGGGTGGAGT GGCTAAAGCG GTTGTCCATT 
GGCCTGCTGA AGGAATCGCC TTCGCACGCA CTGAGAGCCT GTCGTTCGTT GGCCCAAGAG 
TACGATACGC TGTTGAGAGA TCTTTTTAAC GCCGCATTTA TCTCCTGTTG GACGGAACTA 
TCGCCGGATT TGAAAAACGA GCTAACACAG TCACTGATTC AAGCCCTGCA AGTTACCGAC 
ATGCCGGAAA TCACTCAGAC AATCCTCAAC TTGGCCGAGT TTATGGAGCA TTGCGATCGC 
GATCCAATAC CAATAGAAAC TAAGCTATTG GGTACTCGAG CGATGGCTTG CAGAGCGTAT 
GCCAAGGCAT TGCGCTACAA GGAGGAGGAG TTCCTTCTGC GAGAAGATTC CCAAGTTTTT 
GAGTCCTTGA TTCTAATCAA CAACAAGCTG CAGCAAAGAG AAGCAGCGGA AGGTCTGCTC 
ACAAGGTATC GTAATGCAGC CAACGAACTA AATGTTCAAG GAAGATGGTA TGAAAAACTT 
CACAATTGGG ATGAGGCGCT GGAACACTAT GAAAGGAATC TAAAGACGGA TTCCTCGGAC 
TTGGAGGCGC GACTCGGTCA CATGCGCTGC TTGGAGGCCC TCGGAGATTG GTCGGAGCTG 
AGCAACGTGA CCAAGCACGA GTGGGAAAAC TTCGGTACAG AGGCCAAATC AAGAGCAGGA 
CCCTTGGCCG CAGTGGCCGC CTGGGGTCTC CAGGATTGGG AGGCGATGCG GGAGTACGTA 
AGGTGTATAC CAGAGGATAC CCAAGATGGA AGCTACTATC GTGCTGTCCT CGCCGTGCAT 
CATGACGACT TTGAGACCGC ACAGCGCCTT ATTGACGAAA CAAGGGACCT CCTCGACACG 
GAGCTCACAT CAATGGCGGG CGAGTCCTAT GAAAGAGCCT ACGGAGCCAT GGTATGTGTA 
CAAATGCTGG CGGAGCTGGA GGAGGTCATC CAATACAAGC TCATTCCCGA GAGGCGAGAG 
CCGTTAAAGA CCATGTGGTG GAAGCGTCTT CAAGGAGGCC AGCGACTAGT AGAAGACTGG 
CGAAGGATCA TCCAGGTGCA CTCGCTGGTG GTAAAACCGC ACGAGGACAT ACATACCTGG 
CTGAAGTACG CTAGTCTGTG CAGGAAGAGC GGCTCCCTGC ACTTGTCCCA TAAAACTCTG 
GTGATGCTGC TAGGCACCGA TCCCAAGCTT AATCCGAACC AGCCACTGCC TTGTAATCAA 
CCACAGGTGA CCTATGCGTA CACCAAGTAC ATGGCTGCCA ATAATCAGCT GCAGGAAGCC 
TACGAGCAGT TGACGCACTT CGTCAGCACC TATAGTCAGG AGCTAAGCTG TTTACCTCCG 
GAAGCACTAA AGCAACAAGA TCAGCGACTC ATGGCCCGCT GCTATCTGCG CATGGCCACC 
TGGCAGAACA AGCTGCAGGA CAGCATCAGA CCGGATGCCA TTCAAGGCGC ACTCGAATGC 
TTTGAAAAGG CCACCAGCTA TGATCCCAAC TGGTACAAGG CCTGGCACCT GTGGGCGTAC 
ATGAACTTTA AGGTGGTGCA GGCCCAAAAA TCGGCGCTGG ACAAACAACA GCCTCCGGGA 
GCCAGCATGG GAATGACAAT GGGCTCTGGC CTCGACAGTG ATCTAATGAT CATACAGCGA 
TACGCCGTTC CGGCAGTTCA GGGATTCTTC CGTTCAATCA GCCTTATAAA AGGGAATTCG 
CTGCAGGATA CGCTACGTTT GTTAACTCTT TGGTTTGACT ATGGCAACCA TGCAGAGGTG 
TACGAGGCGC TGCTCTCTGG AATGAAGCTG ATCGAGATCA ACACCTGGCT TCAGGTTATC 
CCGCAGCTTA TTGCCCGCAT CGATACCCAC CGTCAGTTGG TGGGTCAATT AATCCACCAA 
CTCCTAATGG ACATAGGAAA GAATCACCCG CAGGCTCTGG TCTATCCTCT AACAGTCGCT 
TCCAAGTCAG CATCATTGGC GCGCAGGAAC GCCGCCTTTA AGATCCTCGA CTCGATGAGG 
AAACACTCGC CCACCTTGGT AGAGCAGGCG GTCATGTGTA GTGAGGAGCT CATCCGTGTG 
GCGATTCTGT GGCACGAACA GTGGCACGAA GGTTTGGAGG AAGCCTCGCG TCTTTACTTT 
GGAGATCGCA ACGTAAAGGG CATGTTTGAG ATCTTGGAGC CACTGCATGC TATGCTGGAG 
AGGGGACCAC AAACACTCAA GGAGACATCC TTCTCGCAGG CCTATGGACG CGAGCTGACA 
GAGGCGTACG AGTGGTCGCA ACGGTACAAG ACATCGGCGG TGGTGATGGA TCTGGATCGA 
GCGTGGGACA TTTACTATCA CGTCTTCCAA AAGATCTCGC GCCAGCTGCC ACAACTTACC 
TCGCTGGAGC TGCCGTATGT CTCGCCCAAA CTGATGACGT GCAAAGATCT CGAACTGGCA 
GTGCCCGGGT CCTACAATCC TGGCCAGGAG CTCATCCGGA TTAGTATCAT TAAAACCAAC 
CTTCAGGTGA TTACTTCCAA GCAGCGGCCG CGAAAACTGT GCATACGCGG CTCCAATGGC 
AAGGACTATA TGTACCTTCT AAAGGGACAC GAGGACCTTC GTCAGGACGA ACGCGTGATG 
CAGCTATTCT CCCTGGTGAA CACATTGCTG TTGGATGACC CCGACACTTT CCGTCGTAAC 
TTGGCCATCC AGCGGTATGC GGTTATCCCG CTCAGTACCA ACTCGGGTCT TATTGGTTGG 
GTGCCACATT GTGACACCCT TCATACGCTA ATCCGGGACT ACCGCGACAA GAAGAAGGTG 
CCACTGAATC AGGAGCATCG CACCATGCTC AATTTTGCGC CAGACTATGA TCACCTCACG 
CTGATGCAGA AGGTGGAAGT GTTCGAGCAT GCGTTGGGTC AGACGCAAGG CGACGACTTG 
GCCAAGTTGC TGTGGCTCAA GTCTCCGTCC TCTGAATTGT GGTTTGAACG GAGAAACAAC 
TATACCCGCT CACTGGCAGT CATGTCAATG GTTGGATATA TCCTAGGTCT GGGCGATCGT 
CATCCATCGA ACCTTATGCT GGATCGTATG AGCGGCAAGA TACTGCACAT AGACTTTGGC 
GACTGCTTCG AGGTGGCCAT GACCCGCGAA AAGTTTCCCG AAAAGATACC CTTCCGTCTC 
ACCCGGATGC TAATAAAGGC AATGGAGGTC ACTGGCATCG AGGGCACCTA CAGACGCACC 
TGCGAAAGCG TGATGCTGGT GCTTCGCCGC AACAAGGATT CCCTGATGGC GGTGCTGGAG 
GCCTTTGTCT ATGATCCGCT GCTCAACTGG AGACTGTTGG ATGTGGACAA GAAGGGTAAC 
GATGCGGTGG CTGGGGCGGG TGCTCCAGGT GGACGTGGCG GTTCCGGAAT GCAGGATAGC 
CTCAGTAACT CCGTAGAGGA CTCCCTGCCC ATGGCCAAGT CGAAGCCCTA CGATCCGACG 
CTACAGCAAG GCGGCCTGCA CAACAACGTG GCCGACGAGA CGAACAGCAA AGCCAGCCAG 
GTAATCAAGC GGGTCAAGTG CAAGCTAACA GGCACCGACT TCCAGACGGA AAAGAGCGTA 
AACGAGCAGT CGCAGGTGGA GCTGCTCATC CAGCAGGCGA CCAATAACGA GAACCTCTGC 
CAGTGCTACA TTGGCTGGTG TCCGTTTTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524891.1
CDS	307..7719
Translation	MSTTSVVQQFVNGLKSRNRNVQNKATQDLLFYVKTELREMSQEELAQFFDEFDHHIFTMVNATDINEKKGGALAMKCLINCEGSLTARKGISPYLNRLRDLLLINDVSVMEIAARSLVKLANMPTSKGADSFDFDIKKAFEVLRGERQEYRRHSAVFILRELAIALPTYFYQHILTFFEVIFNAIFDPKPAIRESAGEALRAALIVTAQRESTKQSSEPQWYRICYDEANGSFNADLGSSKDQKGVTRDDRIHGGLVVFNELFRCANATWERRYTSLKTLFPKTQHNKFLEASSSSSMGSQLNTLVPRLKVPFIDKLGSTQTHLGEGEHHKGVAKFASHNVLESAYAQEILQEHYTSICDNVLEQRTSKSPYVQQALLQILPRLAAFNRAVFVEKYLQTCVSHLMQILRGKEKDRTVAYITIGYMAVAVQSAIEVHLSSIMTSVKVALPSKDLTSKRKVPVDPAVFACITLLAHAVKSEIADDVKDILEQMFYTGLSPALTVCLRELSENVPQLKSAITEGLIGILSQVLMNKAAILPYTALPTIAIDGSLMQNGDGATTVLALKTLGTFNFEEQNMLDFVQRCADYFIVHEQQEIRLEAVQTCTRLLKLAVQSSESMENSKTLSDTVSHVIERLLMVAITDMDCNVRIRILRSLDETFDGKLAQPESLNSLFITLHDEIFEIRELAMVTIGRLSSINPAYVMPKLRTTMIELITDLKYSGMSRNKEQSAKMLDHLVISTPRLISSYMNPILKALVPKLHEPESNPGVILNVLRTIGDLAEVNGGSDEMELWADDLLSILLEMLGDAGSPDKRGVALWTLGQLISATGRVVTPYHKYPVLIDILINFLKTEQRRSIRRETIRVLGLLGAMDPYKHKMNKGLIDSQKDNVLIAYSDGKVDESQDISTAELLVNMGNALDEYYPAVAIAALMRILRDPTLSTRHTSVVQAVTFIFQSLGIKCVPYLAQVLPNLLDNVRTADNNLREFLFQQLAILVAFVKLHIISYMGDIFKLIKEFWTINTPLQNTLINLIEQIAVALGCEFRDYLAELIPQILRVLQHDNSKDRMVTRRLLQALQKFGSTLGYYLPLILPPIVKLFDSPYVPQQVSMVALETINNLACQLDFTDFSSRIIHPLVRVLDAEPELRDQAMTTLRSLAKQLGKKYLVFVPMVQRTLNKHRIVDPEYEELLSKIKSCSTLADSYGAGESELRPSRFKNNEPFVTDRNSNNKNLQVTTNELRTAWQVTRRVSKDDWVEWLKRLSIGLLKESPSHALRACRSLAQEYDTLLRDLFNAAFISCWTELSPDLKNELTQSLIQALQVTDMPEITQTILNLAEFMEHCDRDPIPIETKLLGTRAMACRAYAKALRYKEEEFLLREDSQVFESLILINNKLQQREAAEGLLTRYRNAANELNVQGRWYEKLHNWDEALEHYERNLKTDSSDLEARLGHMRCLEALGDWSELSNVTKHEWENFGTEAKSRAGPLAAVAAWGLQDWEAMREYVRCIPEDTQDGSYYRAVLAVHHDDFETAQRLIDETRDLLDTELTSMAGESYERAYGAMVCVQMLAELEEVIQYKLIPERREPLKTMWWKRLQGGQRLVEDWRRIIQVHSLVVKPHEDIHTWLKYASLCRKSGSLHLSHKTLVMLLGTDPKLNPNQPLPCNQPQVTYAYTKYMAANNQLQEAYEQLTHFVSTYSQELSCLPPEALKQQDQRLMARCYLRMATWQNKLQDSIRPDAIQGALECFEKATSYDPNWYKAWHLWAYMNFKVVQAQKSALDKQQPPGASMGMTMGSGLDSDLMIIQRYAVPAVQGFFRSISLIKGNSLQDTLRLLTLWFDYGNHAEVYEALLSGMKLIEINTWLQVIPQLIARIDTHRQLVGQLIHQLLMDIGKNHPQALVYPLTVASKSASLARRNAAFKILDSMRKHSPTLVEQAVMCSEELIRVAILWHEQWHEGLEEASRLYFGDRNVKGMFEILEPLHAMLERGPQTLKETSFSQAYGRELTEAYEWSQRYKTSAVVMDLDRAWDIYYHVFQKISRQLPQLTSLELPYVSPKLMTCKDLELAVPGSYNPGQELIRISIIKTNLQVITSKQRPRKLCIRGSNGKDYMYLLKGHEDLRQDERVMQLFSLVNTLLLDDPDTFRRNLAIQRYAVIPLSTNSGLIGWVPHCDTLHTLIRDYRDKKKVPLNQEHRTMLNFAPDYDHLTLMQKVEVFEHALGQTQGDDLAKLLWLKSPSSELWFERRNNYTRSLAVMSMVGYILGLGDRHPSNLMLDRMSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLIKAMEVTGIEGTYRRTCESVMLVLRRNKDSLMAVLEAFVYDPLLNWRLLDVDKKGNDAVAGAGAPGGRGGSGMQDSLSNSVEDSLPMAKSKPYDPTLQQGGLHNNVADETNSKASQVIKRVKCKLTGTDFQTEKSVNEQSQVELLIQQATNNENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	NM_080152.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of rapamycin, transcript variant A (Tor), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080152.3

ORF Insert Sequence:

ATGTCGACGA CGTCGGTGGT GCAGCAGTTC GTCAACGGGC TTAAGTCCCG CAACCGGAAT 
GTACAGAACA AGGCAACCCA GGATCTGCTC TTCTATGTGA AGACCGAGCT ACGCGAAATG 
TCCCAGGAGG AGCTGGCCCA ATTTTTCGAC GAGTTCGACC ATCACATTTT CACGATGGTG 
AATGCGACAG ATATCAACGA GAAAAAAGGC GGTGCCCTGG CCATGAAGTG CCTGATTAAC 
TGCGAGGGCA GTCTTACTGC CAGGAAGGGC ATTTCGCCCT ATCTCAACCG CCTGCGCGAT 
CTTCTGCTAA TCAACGATGT GTCCGTCATG GAGATTGCCG CCCGCTCCCT GGTCAAATTG 
GCCAACATGC CCACCTCCAA GGGCGCCGAT TCCTTCGACT TTGATATTAA GAAGGCTTTT 
GAGGTGCTCA GAGGCGAGAG ACAAGAGTAC CGCCGCCATT CAGCCGTCTT TATCCTCCGT 
GAGCTGGCTA TCGCCTTGCC CACATACTTC TACCAGCACA TCCTGACCTT CTTCGAGGTT 
ATCTTTAACG CCATCTTCGA TCCCAAACCT GCTATACGAG AGTCAGCTGG CGAGGCTTTG 
AGGGCGGCTT TGATTGTCAC CGCTCAGCGA GAGAGCACCA AGCAGTCCAG TGAACCACAG 
TGGTACAGAA TCTGCTATGA CGAGGCGAAT GGAAGCTTCA ATGCAGACTT GGGCTCTAGC 
AAGGATCAGA AAGGCGTGAC CCGAGACGAT CGCATTCACG GTGGCCTCGT CGTCTTCAAC 
GAGCTCTTTC GCTGTGCCAA TGCCACCTGG GAGCGGCGCT ACACGTCTCT GAAGACGCTG 
TTCCCCAAGA CCCAACACAA TAAGTTCCTG GAGGCCAGCA GTTCGTCGAG TATGGGTTCC 
CAGCTGAATA CTCTAGTGCC GCGGCTAAAG GTGCCCTTCA TTGACAAGCT CGGCAGCACC 
CAAACGCATT TGGGTGAGGG AGAGCATCAC AAAGGAGTTG CCAAGTTCGC GAGCCACAAC 
GTACTGGAGT CCGCATATGC TCAGGAGATC CTTCAGGAGC ACTACACCTC CATTTGCGAC 
AATGTTTTGG AGCAGCGAAC TTCGAAGTCT CCGTATGTAC AGCAGGCACT CCTTCAAATA 
CTTCCTCGAT TGGCTGCCTT TAACCGCGCG GTGTTTGTGG AAAAATACCT GCAGACATGC 
GTGTCGCATT TAATGCAGAT CCTGCGAGGC AAGGAGAAGG ATCGCACGGT GGCGTACATA 
ACCATTGGTT ACATGGCTGT AGCGGTGCAG AGTGCGATCG AAGTTCATTT ATCCAGTATA 
ATGACTAGCG TGAAGGTCGC CTTGCCGTCA AAGGATTTGA CCAGCAAACG AAAGGTACCA 
GTGGATCCTG CGGTCTTTGC CTGCATAACG CTTCTTGCTC ATGCCGTTAA GTCGGAGATC 
GCCGACGATG TTAAGGATAT CCTTGAGCAG ATGTTCTACA CGGGTCTCTC GCCAGCTCTG 
ACTGTTTGCC TGAGGGAACT CTCCGAGAAT GTGCCGCAGC TTAAGTCGGC CATTACAGAA 
GGATTGATCG GAATCCTATC CCAGGTGCTG ATGAACAAGG CGGCAATTTT ACCATACACT 
GCTTTGCCAA CGATCGCCAT CGATGGCTCG CTAATGCAGA ATGGTGACGG AGCAACCACA 
GTGCTGGCAC TCAAAACGCT GGGTACCTTC AACTTTGAGG AGCAAAACAT GCTGGACTTT 
GTGCAACGAT GTGCTGACTA CTTCATCGTT CACGAGCAGC AGGAGATTCG TCTGGAGGCA 
GTGCAAACAT GCACACGACT CCTTAAACTG GCAGTGCAAT CCTCTGAAAG TATGGAGAAC 
TCCAAGACCC TCAGCGACAC CGTTTCCCAC GTGATTGAGC GCCTGCTTAT GGTAGCCATT 
ACTGATATGG ACTGTAATGT GCGAATACGA ATTCTACGTT CGCTGGACGA GACCTTTGAT 
GGGAAGCTTG CTCAGCCAGA GTCTCTAAAT TCGCTATTTA TTACGCTGCA CGATGAAATC 
TTTGAGATCC GGGAGCTGGC AATGGTTACC ATTGGAAGAT TGTCGTCCAT AAACCCGGCT 
TATGTGATGC CCAAGCTAAG AACCACCATG ATCGAATTGA TCACGGATCT AAAGTACTCG 
GGAATGAGTC GAAACAAGGA GCAGAGCGCA AAGATGCTTG ACCATTTGGT GATAAGCACG 
CCCAGGCTAA TATCCTCTTA CATGAATCCG ATCCTCAAAG CCTTAGTGCC CAAGTTGCAT 
GAGCCGGAAT CGAATCCAGG AGTTATTTTA AATGTGCTTC GCACAATTGG TGATTTAGCA 
GAGGTTAATG GAGGCTCCGA TGAAATGGAA TTGTGGGCAG ATGACCTTCT GTCCATTTTA 
CTCGAAATGC TGGGCGACGC TGGCAGTCCG GATAAGCGAG GAGTGGCTCT GTGGACGTTG 
GGACAGTTAA TCAGTGCAAC AGGCAGGGTG GTTACCCCAT ATCACAAGTA CCCCGTGCTC 
ATAGATATTT TGATTAATTT CCTTAAAACG GAACAACGTC GATCCATTCG AAGGGAAACG 
ATACGAGTGC TGGGCTTGTT GGGAGCCATG GATCCTTACA AGCACAAGAT GAACAAGGGC 
TTGATTGATA GCCAAAAGGA TAATGTTTTA ATAGCATACT CCGATGGCAA AGTCGATGAA 
AGTCAGGACA TCTCCACCGC TGAGTTGCTA GTTAACATGG GTAACGCATT GGATGAGTAT 
TATCCCGCTG TGGCCATAGC TGCATTGATG AGGATTCTAC GGGATCCCAC GTTAAGTACA 
CGTCACACCA GCGTGGTGCA GGCGGTTACT TTTATCTTCC AGAGCCTGGG CATCAAATGT 
GTGCCGTACT TGGCCCAAGT GCTTCCCAAT TTGCTGGACA ATGTACGCAC TGCAGATAAT 
AATCTAAGGG AGTTTCTCTT CCAGCAGCTC GCCATTCTGG TGGCTTTTGT CAAGCTGCAT 
ATCATCAGCT ACATGGGAGA TATTTTTAAG CTGATCAAAG AATTTTGGAC CATTAATACG 
CCTCTGCAAA ACACTCTAAT CAATTTGATT GAACAGATCG CCGTGGCTTT GGGATGCGAG 
TTTAGGGATT ATTTGGCGGA GCTGATCCCA CAAATTCTGC GAGTGTTGCA GCACGATAAC 
TCCAAGGATA GAATGGTCAC GAGAAGGTTG CTCCAAGCTC TTCAAAAGTT TGGCAGCACT 
TTGGGTTACT ATCTGCCGCT GATTTTGCCT CCGATCGTGA AACTCTTCGA TTCTCCGTAC 
GTACCACAAC AGGTGTCAAT GGTAGCCCTG GAAACGATCA ACAATCTAGC CTGTCAGCTG 
GACTTCACCG ACTTCTCCTC GCGCATCATA CATCCGTTGG TTCGAGTGCT GGACGCTGAG 
CCAGAGCTTC GGGACCAAGC TATGACAACC CTTCGCTCAC TGGCAAAGCA ACTGGGCAAG 
AAGTACCTCG TGTTCGTGCC TATGGTCCAG CGCACTCTCA ACAAACATCG CATTGTGGAT 
CCCGAGTACG AGGAGCTACT GAGTAAGATT AAGTCCTGCA GCACACTGGC GGATTCCTAT 
GGGGCCGGGG AGTCAGAACT GCGGCCGTCC AGGTTCAAAA ACAACGAGCC ATTTGTGACC 
GACAGAAATA GCAATAATAA AAATCTGCAG GTCACCACAA ACGAACTACG AACGGCGTGG 
CAGGTGACGC GGCGGGTGTC CAAGGACGAC TGGGTGGAGT GGCTAAAGCG GTTGTCCATT 
GGCCTGCTGA AGGAATCGCC TTCGCACGCA CTGAGAGCCT GTCGTTCGTT GGCCCAAGAG 
TACGATACGC TGTTGAGAGA TCTTTTTAAC GCCGCATTTA TCTCCTGTTG GACGGAACTA 
TCGCCGGATT TGAAAAACGA GCTAACACAG TCACTGATTC AAGCCCTGCA AGTTACCGAC 
ATGCCGGAAA TCACTCAGAC AATCCTCAAC TTGGCCGAGT TTATGGAGCA TTGCGATCGC 
GATCCAATAC CAATAGAAAC TAAGCTATTG GGTACTCGAG CGATGGCTTG CAGAGCGTAT 
GCCAAGGCAT TGCGCTACAA GGAGGAGGAG TTCCTTCTGC GAGAAGATTC CCAAGTTTTT 
GAGTCCTTGA TTCTAATCAA CAACAAGCTG CAGCAAAGAG AAGCAGCGGA AGGTCTGCTC 
ACAAGGTATC GTAATGCAGC CAACGAACTA AATGTTCAAG GAAGATGGTA TGAAAAACTT 
CACAATTGGG ATGAGGCGCT GGAACACTAT GAAAGGAATC TAAAGACGGA TTCCTCGGAC 
TTGGAGGCGC GACTCGGTCA CATGCGCTGC TTGGAGGCCC TCGGAGATTG GTCGGAGCTG 
AGCAACGTGA CCAAGCACGA GTGGGAAAAC TTCGGTACAG AGGCCAAATC AAGAGCAGGA 
CCCTTGGCCG CAGTGGCCGC CTGGGGTCTC CAGGATTGGG AGGCGATGCG GGAGTACGTA 
AGGTGTATAC CAGAGGATAC CCAAGATGGA AGCTACTATC GTGCTGTCCT CGCCGTGCAT 
CATGACGACT TTGAGACCGC ACAGCGCCTT ATTGACGAAA CAAGGGACCT CCTCGACACG 
GAGCTCACAT CAATGGCGGG CGAGTCCTAT GAAAGAGCCT ACGGAGCCAT GGTATGTGTA 
CAAATGCTGG CGGAGCTGGA GGAGGTCATC CAATACAAGC TCATTCCCGA GAGGCGAGAG 
CCGTTAAAGA CCATGTGGTG GAAGCGTCTT CAAGGAGGCC AGCGACTAGT AGAAGACTGG 
CGAAGGATCA TCCAGGTGCA CTCGCTGGTG GTAAAACCGC ACGAGGACAT ACATACCTGG 
CTGAAGTACG CTAGTCTGTG CAGGAAGAGC GGCTCCCTGC ACTTGTCCCA TAAAACTCTG 
GTGATGCTGC TAGGCACCGA TCCCAAGCTT AATCCGAACC AGCCACTGCC TTGTAATCAA 
CCACAGGTGA CCTATGCGTA CACCAAGTAC ATGGCTGCCA ATAATCAGCT GCAGGAAGCC 
TACGAGCAGT TGACGCACTT CGTCAGCACC TATAGTCAGG AGCTAAGCTG TTTACCTCCG 
GAAGCACTAA AGCAACAAGA TCAGCGACTC ATGGCCCGCT GCTATCTGCG CATGGCCACC 
TGGCAGAACA AGCTGCAGGA CAGCATCAGA CCGGATGCCA TTCAAGGCGC ACTCGAATGC 
TTTGAAAAGG CCACCAGCTA TGATCCCAAC TGGTACAAGG CCTGGCACCT GTGGGCGTAC 
ATGAACTTTA AGGTGGTGCA GGCCCAAAAA TCGGCGCTGG ACAAACAACA GCCTCCGGGA 
GCCAGCATGG GAATGACAAT GGGCTCTGGC CTCGACAGTG ATCTAATGAT CATACAGCGA 
TACGCCGTTC CGGCAGTTCA GGGATTCTTC CGTTCAATCA GCCTTATAAA AGGGAATTCG 
CTGCAGGATA CGCTACGTTT GTTAACTCTT TGGTTTGACT ATGGCAACCA TGCAGAGGTG 
TACGAGGCGC TGCTCTCTGG AATGAAGCTG ATCGAGATCA ACACCTGGCT TCAGGTTATC 
CCGCAGCTTA TTGCCCGCAT CGATACCCAC CGTCAGTTGG TGGGTCAATT AATCCACCAA 
CTCCTAATGG ACATAGGAAA GAATCACCCG CAGGCTCTGG TCTATCCTCT AACAGTCGCT 
TCCAAGTCAG CATCATTGGC GCGCAGGAAC GCCGCCTTTA AGATCCTCGA CTCGATGAGG 
AAACACTCGC CCACCTTGGT AGAGCAGGCG GTCATGTGTA GTGAGGAGCT CATCCGTGTG 
GCGATTCTGT GGCACGAACA GTGGCACGAA GGTTTGGAGG AAGCCTCGCG TCTTTACTTT 
GGAGATCGCA ACGTAAAGGG CATGTTTGAG ATCTTGGAGC CACTGCATGC TATGCTGGAG 
AGGGGACCAC AAACACTCAA GGAGACATCC TTCTCGCAGG CCTATGGACG CGAGCTGACA 
GAGGCGTACG AGTGGTCGCA ACGGTACAAG ACATCGGCGG TGGTGATGGA TCTGGATCGA 
GCGTGGGACA TTTACTATCA CGTCTTCCAA AAGATCTCGC GCCAGCTGCC ACAACTTACC 
TCGCTGGAGC TGCCGTATGT CTCGCCCAAA CTGATGACGT GCAAAGATCT CGAACTGGCA 
GTGCCCGGGT CCTACAATCC TGGCCAGGAG CTCATCCGGA TTAGTATCAT TAAAACCAAC 
CTTCAGGTGA TTACTTCCAA GCAGCGGCCG CGAAAACTGT GCATACGCGG CTCCAATGGC 
AAGGACTATA TGTACCTTCT AAAGGGACAC GAGGACCTTC GTCAGGACGA ACGCGTGATG 
CAGCTATTCT CCCTGGTGAA CACATTGCTG TTGGATGACC CCGACACTTT CCGTCGTAAC 
TTGGCCATCC AGCGGTATGC GGTTATCCCG CTCAGTACCA ACTCGGGTCT TATTGGTTGG 
GTGCCACATT GTGACACCCT TCATACGCTA ATCCGGGACT ACCGCGACAA GAAGAAGGTG 
CCACTGAATC AGGAGCATCG CACCATGCTC AATTTTGCGC CAGACTATGA TCACCTCACG 
CTGATGCAGA AGGTGGAAGT GTTCGAGCAT GCGTTGGGTC AGACGCAAGG CGACGACTTG 
GCCAAGTTGC TGTGGCTCAA GTCTCCGTCC TCTGAATTGT GGTTTGAACG GAGAAACAAC 
TATACCCGCT CACTGGCAGT CATGTCAATG GTTGGATATA TCCTAGGTCT GGGCGATCGT 
CATCCATCGA ACCTTATGCT GGATCGTATG AGCGGCAAGA TACTGCACAT AGACTTTGGC 
GACTGCTTCG AGGTGGCCAT GACCCGCGAA AAGTTTCCCG AAAAGATACC CTTCCGTCTC 
ACCCGGATGC TAATAAAGGC AATGGAGGTC ACTGGCATCG AGGGCACCTA CAGACGCACC 
TGCGAAAGCG TGATGCTGGT GCTTCGCCGC AACAAGGATT CCCTGATGGC GGTGCTGGAG 
GCCTTTGTCT ATGATCCGCT GCTCAACTGG AGACTGTTGG ATGTGGACAA GAAGGGTAAC 
GATGCGGTGG CTGGGGCGGG TGCTCCAGGT GGACGTGGCG GTTCCGGAAT GCAGGATAGC 
CTCAGTAACT CCGTAGAGGA CTCCCTGCCC ATGGCCAAGT CGAAGCCCTA CGATCCGACG 
CTACAGCAAG GCGGCCTGCA CAACAACGTG GCCGACGAGA CGAACAGCAA AGCCAGCCAG 
GTAATCAAGC GGGTCAAGTG CAAGCTAACA GGCACCGACT TCCAGACGGA AAAGAGCGTA 
AACGAGCAGT CGCAGGTGGA GCTGCTCATC CAGCAGGCGA CCAATAACGA GAACCTCTGC 
CAGTGCTACA TTGGCTGGTG TCCGTTTTGG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tor ( NM_001273498.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260427.1 Drosophila melanogaster target of rapamycin, transcript variant B (Tor), mRNA.

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CloneID	OFa16861
Clone ID Related Accession (Same CDS sequence)	NM_001273498.1
Accession Version	NM_001273498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of rapamycin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGACGA CGTCGGTGGT GCAGCAGTTC GTCAACGGGC TTAAGTCCCG CAACCGGAAT 
GTACAGAACA AGGCAACCCA GGATCTGCTC TTCTATGTGA AGACCGAGCT ACGCGAAATG 
TCCCAGGAGG AGCTGGCCCA ATTTTTCGAC GAGTTCGACC ATCACATTTT CACGATGGTG 
AATGCGACAG ATATCAACGA GAAAAAAGGC GGTGCCCTGG CCATGAAGTG CCTGATTAAC 
TGCGAGGGCA GTCTTACTGC CAGGAAGGGC ATTTCGCCCT ATCTCAACCG CCTGCGCGAT 
CTTCTGCTAA TCAACGATGT GTCCGTCATG GAGATTGCCG CCCGCTCCCT GGTCAAATTG 
GCCAACATGC CCACCTCCAA GGGCGCCGAT TCCTTCGACT TTGATATTAA GAAGGCTTTT 
GAGGTGCTCA GAGGCGAGAG ACAAGAGTAC CGCCGCCATT CAGCCGTCTT TATCCTCCGT 
GAGCTGGCTA TCGCCTTGCC CACATACTTC TACCAGCACA TCCTGACCTT CTTCGAGGTT 
ATCTTTAACG CCATCTTCGA TCCCAAACCT GCTATACGAG AGTCAGCTGG CGAGGCTTTG 
AGGGCGGCTT TGATTGTCAC CGCTCAGCGA GAGAGCACCA AGCAGTCCAG TGAACCACAG 
TGGTACAGAA TCTGCTATGA CGAGGCGAAT GGAAGCTTCA ATGCAGACTT GGGCTCTAGC 
AAGGATCAGA AAGGCGTGAC CCGAGACGAT CGCATTCACG GTGGCCTCGT CGTCTTCAAC 
GAGCTCTTTC GCTGTGCCAA TGCCACCTGG GAGCGGCGCT ACACGTCTCT GAAGACGCTG 
TTCCCCAAGA CCCAACACAA TAAGTTCCTG GAGGCCAGCA GTTCGTCGAG TATGGGTTCC 
CAGCTGAATA CTCTAGTGCC GCGGCTAAAG GTGCCCTTCA TTGACAAGCT CGGCAGCACC 
CAAACGCATT TGGGTGAGGG AGAGCATCAC AAAGGAGTTG CCAAGTTCGC GAGCCAGCAC 
AACGTACTGG AGTCCGCATA TGCTCAGGAG ATCCTTCAGG AGCACTACAC CTCCATTTGC 
GACAATGTTT TGGAGCAGCG AACTTCGAAG TCTCCGTATG TACAGCAGGC ACTCCTTCAA 
ATACTTCCTC GATTGGCTGC CTTTAACCGC GCGGTGTTTG TGGAAAAATA CCTGCAGACA 
TGCGTGTCGC ATTTAATGCA GATCCTGCGA GGCAAGGAGA AGGATCGCAC GGTGGCGTAC 
ATAACCATTG GTTACATGGC TGTAGCGGTG CAGAGTGCGA TCGAAGTTCA TTTATCCAGT 
ATAATGACTA GCGTGAAGGT CGCCTTGCCG TCAAAGGATT TGACCAGCAA ACGAAAGGTA 
CCAGTGGATC CTGCGGTCTT TGCCTGCATA ACGCTTCTTG CTCATGCCGT TAAGTCGGAG 
ATCGCCGACG ATGTTAAGGA TATCCTTGAG CAGATGTTCT ACACGGGTCT CTCGCCAGCT 
CTGACTGTTT GCCTGAGGGA ACTCTCCGAG AATGTGCCGC AGCTTAAGTC GGCCATTACA 
GAAGGATTGA TCGGAATCCT ATCCCAGGTG CTGATGAACA AGGCGGCAAT TTTACCATAC 
ACTGCTTTGC CAACGATCGC CATCGATGGC TCGCTAATGC AGAATGGTGA CGGAGCAACC 
ACAGTGCTGG CACTCAAAAC GCTGGGTACC TTCAACTTTG AGGAGCAAAA CATGCTGGAC 
TTTGTGCAAC GATGTGCTGA CTACTTCATC GTTCACGAGC AGCAGGAGAT TCGTCTGGAG 
GCAGTGCAAA CATGCACACG ACTCCTTAAA CTGGCAGTGC AATCCTCTGA AAGTATGGAG 
AACTCCAAGA CCCTCAGCGA CACCGTTTCC CACGTGATTG AGCGCCTGCT TATGGTAGCC 
ATTACTGATA TGGACTGTAA TGTGCGAATA CGAATTCTAC GTTCGCTGGA CGAGACCTTT 
GATGGGAAGC TTGCTCAGCC AGAGTCTCTA AATTCGCTAT TTATTACGCT GCACGATGAA 
ATCTTTGAGA TCCGGGAGCT GGCAATGGTT ACCATTGGAA GATTGTCGTC CATAAACCCG 
GCTTATGTGA TGCCCAAGCT AAGAACCACC ATGATCGAAT TGATCACGGA TCTAAAGTAC 
TCGGGAATGA GTCGAAACAA GGAGCAGAGC GCAAAGATGC TTGACCATTT GGTGATAAGC 
ACGCCCAGGC TAATATCCTC TTACATGAAT CCGATCCTCA AAGCCTTAGT GCCCAAGTTG 
CATGAGCCGG AATCGAATCC AGGAGTTATT TTAAATGTGC TTCGCACAAT TGGTGATTTA 
GCAGAGGTTA ATGGAGGCTC CGATGAAATG GAATTGTGGG CAGATGACCT TCTGTCCATT 
TTACTCGAAA TGCTGGGCGA CGCTGGCAGT CCGGATAAGC GAGGAGTGGC TCTGTGGACG 
TTGGGACAGT TAATCAGTGC AACAGGCAGG GTGGTTACCC CATATCACAA GTACCCCGTG 
CTCATAGATA TTTTGATTAA TTTCCTTAAA ACGGAACAAC GTCGATCCAT TCGAAGGGAA 
ACGATACGAG TGCTGGGCTT GTTGGGAGCC ATGGATCCTT ACAAGCACAA GATGAACAAG 
GGCTTGATTG ATAGCCAAAA GGATAATGTT TTAATAGCAT ACTCCGATGG CAAAGTCGAT 
GAAAGTCAGG ACATCTCCAC CGCTGAGTTG CTAGTTAACA TGGGTAACGC ATTGGATGAG 
TATTATCCCG CTGTGGCCAT AGCTGCATTG ATGAGGATTC TACGGGATCC CACGTTAAGT 
ACACGTCACA CCAGCGTGGT GCAGGCGGTT ACTTTTATCT TCCAGAGCCT GGGCATCAAA 
TGTGTGCCGT ACTTGGCCCA AGTGCTTCCC AATTTGCTGG ACAATGTACG CACTGCAGAT 
AATAATCTAA GGGAGTTTCT CTTCCAGCAG CTCGCCATTC TGGTGGCTTT TGTCAAGCTG 
CATATCATCA GCTACATGGG AGATATTTTT AAGCTGATCA AAGAATTTTG GACCATTAAT 
ACGCCTCTGC AAAACACTCT AATCAATTTG ATTGAACAGA TCGCCGTGGC TTTGGGATGC 
GAGTTTAGGG ATTATTTGGC GGAGCTGATC CCACAAATTC TGCGAGTGTT GCAGCACGAT 
AACTCCAAGG ATAGAATGGT CACGAGAAGG TTGCTCCAAG CTCTTCAAAA GTTTGGCAGC 
ACTTTGGGTT ACTATCTGCC GCTGATTTTG CCTCCGATCG TGAAACTCTT CGATTCTCCG 
TACGTACCAC AACAGGTGTC AATGGTAGCC CTGGAAACGA TCAACAATCT AGCCTGTCAG 
CTGGACTTCA CCGACTTCTC CTCGCGCATC ATACATCCGT TGGTTCGAGT GCTGGACGCT 
GAGCCAGAGC TTCGGGACCA AGCTATGACA ACCCTTCGCT CACTGGCAAA GCAACTGGGC 
AAGAAGTACC TCGTGTTCGT GCCTATGGTC CAGCGCACTC TCAACAAACA TCGCATTGTG 
GATCCCGAGT ACGAGGAGCT ACTGAGTAAG ATTAAGTCCT GCAGCACACT GGCGGATTCC 
TATGGGGCCG GGGAGTCAGA ACTGCGGCCG TCCAGGTTCA AAAACAACGA GCCATTTGTG 
ACCGACAGAA ATAGCAATAA TAAAAATCTG CAGGTCACCA CAAACGAACT ACGAACGGCG 
TGGCAGGTGA CGCGGCGGGT GTCCAAGGAC GACTGGGTGG AGTGGCTAAA GCGGTTGTCC 
ATTGGCCTGC TGAAGGAATC GCCTTCGCAC GCACTGAGAG CCTGTCGTTC GTTGGCCCAA 
GAGTACGATA CGCTGTTGAG AGATCTTTTT AACGCCGCAT TTATCTCCTG TTGGACGGAA 
CTATCGCCGG ATTTGAAAAA CGAGCTAACA CAGTCACTGA TTCAAGCCCT GCAAGTTACC 
GACATGCCGG AAATCACTCA GACAATCCTC AACTTGGCCG AGTTTATGGA GCATTGCGAT 
CGCGATCCAA TACCAATAGA AACTAAGCTA TTGGGTACTC GAGCGATGGC TTGCAGAGCG 
TATGCCAAGG CATTGCGCTA CAAGGAGGAG GAGTTCCTTC TGCGAGAAGA TTCCCAAGTT 
TTTGAGTCCT TGATTCTAAT CAACAACAAG CTGCAGCAAA GAGAAGCAGC GGAAGGTCTG 
CTCACAAGGT ATCGTAATGC AGCCAACGAA CTAAATGTTC AAGGAAGATG GTATGAAAAA 
CTTCACAATT GGGATGAGGC GCTGGAACAC TATGAAAGGA ATCTAAAGAC GGATTCCTCG 
GACTTGGAGG CGCGACTCGG TCACATGCGC TGCTTGGAGG CCCTCGGAGA TTGGTCGGAG 
CTGAGCAACG TGACCAAGCA CGAGTGGGAA AACTTCGGTA CAGAGGCCAA ATCAAGAGCA 
GGACCCTTGG CCGCAGTGGC CGCCTGGGGT CTCCAGGATT GGGAGGCGAT GCGGGAGTAC 
GTAAGGTGTA TACCAGAGGA TACCCAAGAT GGAAGCTACT ATCGTGCTGT CCTCGCCGTG 
CATCATGACG ACTTTGAGAC CGCACAGCGC CTTATTGACG AAACAAGGGA CCTCCTCGAC 
ACGGAGCTCA CATCAATGGC GGGCGAGTCC TATGAAAGAG CCTACGGAGC CATGGTATGT 
GTACAAATGC TGGCGGAGCT GGAGGAGGTC ATCCAATACA AGCTCATTCC CGAGAGGCGA 
GAGCCGTTAA AGACCATGTG GTGGAAGCGT CTTCAAGGAG GCCAGCGACT AGTAGAAGAC 
TGGCGAAGGA TCATCCAGGT GCACTCGCTG GTGGTAAAAC CGCACGAGGA CATACATACC 
TGGCTGAAGT ACGCTAGTCT GTGCAGGAAG AGCGGCTCCC TGCACTTGTC CCATAAAACT 
CTGGTGATGC TGCTAGGCAC CGATCCCAAG CTTAATCCGA ACCAGCCACT GCCTTGTAAT 
CAACCACAGG TGACCTATGC GTACACCAAG TACATGGCTG CCAATAATCA GCTGCAGGAA 
GCCTACGAGC AGTTGACGCA CTTCGTCAGC ACCTATAGTC AGGAGCTAAG CTGTTTACCT 
CCGGAAGCAC TAAAGCAACA AGATCAGCGA CTCATGGCCC GCTGCTATCT GCGCATGGCC 
ACCTGGCAGA ACAAGCTGCA GGACAGCATC AGACCGGATG CCATTCAAGG CGCACTCGAA 
TGCTTTGAAA AGGCCACCAG CTATGATCCC AACTGGTACA AGGCCTGGCA CCTGTGGGCG 
TACATGAACT TTAAGGTGGT GCAGGCCCAA AAATCGGCGC TGGACAAACA ACAGCCTCCG 
GGAGCCAGCA TGGGAATGAC AATGGGCTCT GGCCTCGACA GTGATCTAAT GATCATACAG 
CGATACGCCG TTCCGGCAGT TCAGGGATTC TTCCGTTCAA TCAGCCTTAT AAAAGGGAAT 
TCGCTGCAGG ATACGCTACG TTTGTTAACT CTTTGGTTTG ACTATGGCAA CCATGCAGAG 
GTGTACGAGG CGCTGCTCTC TGGAATGAAG CTGATCGAGA TCAACACCTG GCTTCAGGTT 
ATCCCGCAGC TTATTGCCCG CATCGATACC CACCGTCAGT TGGTGGGTCA ATTAATCCAC 
CAACTCCTAA TGGACATAGG AAAGAATCAC CCGCAGGCTC TGGTCTATCC TCTAACAGTC 
GCTTCCAAGT CAGCATCATT GGCGCGCAGG AACGCCGCCT TTAAGATCCT CGACTCGATG 
AGGAAACACT CGCCCACCTT GGTAGAGCAG GCGGTCATGT GTAGTGAGGA GCTCATCCGT 
GTGGCGATTC TGTGGCACGA ACAGTGGCAC GAAGGTTTGG AGGAAGCCTC GCGTCTTTAC 
TTTGGAGATC GCAACGTAAA GGGCATGTTT GAGATCTTGG AGCCACTGCA TGCTATGCTG 
GAGAGGGGAC CACAAACACT CAAGGAGACA TCCTTCTCGC AGGCCTATGG ACGCGAGCTG 
ACAGAGGCGT ACGAGTGGTC GCAACGGTAC AAGACATCGG CGGTGGTGAT GGATCTGGAT 
CGAGCGTGGG ACATTTACTA TCACGTCTTC CAAAAGATCT CGCGCCAGCT GCCACAACTT 
ACCTCGCTGG AGCTGCCGTA TGTCTCGCCC AAACTGATGA CGTGCAAAGA TCTCGAACTG 
GCAGTGCCCG GGTCCTACAA TCCTGGCCAG GAGCTCATCC GGATTAGTAT CATTAAAACC 
AACCTTCAGG TGATTACTTC CAAGCAGCGG CCGCGAAAAC TGTGCATACG CGGCTCCAAT 
GGCAAGGACT ATATGTACCT TCTAAAGGGA CACGAGGACC TTCGTCAGGA CGAACGCGTG 
ATGCAGCTAT TCTCCCTGGT GAACACATTG CTGTTGGATG ACCCCGACAC TTTCCGTCGT 
AACTTGGCCA TCCAGCGGTA TGCGGTTATC CCGCTCAGTA CCAACTCGGG TCTTATTGGT 
TGGGTGCCAC ATTGTGACAC CCTTCATACG CTAATCCGGG ACTACCGCGA CAAGAAGAAG 
GTGCCACTGA ATCAGGAGCA TCGCACCATG CTCAATTTTG CGCCAGACTA TGATCACCTC 
ACGCTGATGC AGAAGGTGGA AGTGTTCGAG CATGCGTTGG GTCAGACGCA AGGCGACGAC 
TTGGCCAAGT TGCTGTGGCT CAAGTCTCCG TCCTCTGAAT TGTGGTTTGA ACGGAGAAAC 
AACTATACCC GCTCACTGGC AGTCATGTCA ATGGTTGGAT ATATCCTAGG TCTGGGCGAT 
CGTCATCCAT CGAACCTTAT GCTGGATCGT ATGAGCGGCA AGATACTGCA CATAGACTTT 
GGCGACTGCT TCGAGGTGGC CATGACCCGC GAAAAGTTTC CCGAAAAGAT ACCCTTCCGT 
CTCACCCGGA TGCTAATAAA GGCAATGGAG GTCACTGGCA TCGAGGGCAC CTACAGACGC 
ACCTGCGAAA GCGTGATGCT GGTGCTTCGC CGCAACAAGG ATTCCCTGAT GGCGGTGCTG 
GAGGCCTTTG TCTATGATCC GCTGCTCAAC TGGAGACTGT TGGATGTGGA CAAGAAGGGT 
AACGATGCGG TGGCTGGGGC GGGTGCTCCA GGTGGACGTG GCGGTTCCGG AATGCAGGAT 
AGCCTCAGTA ACTCCGTAGA GGACTCCCTG CCCATGGCCA AGTCGAAGCC CTACGATCCG 
ACGCTACAGC AAGGCGGCCT GCACAACAAC GTGGCCGACG AGACGAACAG CAAAGCCAGC 
CAGGTAATCA AGCGGGTCAA GTGCAAGCTA ACAGGCACCG ACTTCCAGAC GGAAAAGAGC 
GTAAACGAGC AGTCGCAGGT GGAGCTGCTC ATCCAGCAGG CGACCAATAA CGAGAACCTC 
TGCCAGTGCT ACATTGGCTG GTGTCCGTTT TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260427.1
CDS	307..7722
Translation	MSTTSVVQQFVNGLKSRNRNVQNKATQDLLFYVKTELREMSQEELAQFFDEFDHHIFTMVNATDINEKKGGALAMKCLINCEGSLTARKGISPYLNRLRDLLLINDVSVMEIAARSLVKLANMPTSKGADSFDFDIKKAFEVLRGERQEYRRHSAVFILRELAIALPTYFYQHILTFFEVIFNAIFDPKPAIRESAGEALRAALIVTAQRESTKQSSEPQWYRICYDEANGSFNADLGSSKDQKGVTRDDRIHGGLVVFNELFRCANATWERRYTSLKTLFPKTQHNKFLEASSSSSMGSQLNTLVPRLKVPFIDKLGSTQTHLGEGEHHKGVAKFASQHNVLESAYAQEILQEHYTSICDNVLEQRTSKSPYVQQALLQILPRLAAFNRAVFVEKYLQTCVSHLMQILRGKEKDRTVAYITIGYMAVAVQSAIEVHLSSIMTSVKVALPSKDLTSKRKVPVDPAVFACITLLAHAVKSEIADDVKDILEQMFYTGLSPALTVCLRELSENVPQLKSAITEGLIGILSQVLMNKAAILPYTALPTIAIDGSLMQNGDGATTVLALKTLGTFNFEEQNMLDFVQRCADYFIVHEQQEIRLEAVQTCTRLLKLAVQSSESMENSKTLSDTVSHVIERLLMVAITDMDCNVRIRILRSLDETFDGKLAQPESLNSLFITLHDEIFEIRELAMVTIGRLSSINPAYVMPKLRTTMIELITDLKYSGMSRNKEQSAKMLDHLVISTPRLISSYMNPILKALVPKLHEPESNPGVILNVLRTIGDLAEVNGGSDEMELWADDLLSILLEMLGDAGSPDKRGVALWTLGQLISATGRVVTPYHKYPVLIDILINFLKTEQRRSIRRETIRVLGLLGAMDPYKHKMNKGLIDSQKDNVLIAYSDGKVDESQDISTAELLVNMGNALDEYYPAVAIAALMRILRDPTLSTRHTSVVQAVTFIFQSLGIKCVPYLAQVLPNLLDNVRTADNNLREFLFQQLAILVAFVKLHIISYMGDIFKLIKEFWTINTPLQNTLINLIEQIAVALGCEFRDYLAELIPQILRVLQHDNSKDRMVTRRLLQALQKFGSTLGYYLPLILPPIVKLFDSPYVPQQVSMVALETINNLACQLDFTDFSSRIIHPLVRVLDAEPELRDQAMTTLRSLAKQLGKKYLVFVPMVQRTLNKHRIVDPEYEELLSKIKSCSTLADSYGAGESELRPSRFKNNEPFVTDRNSNNKNLQVTTNELRTAWQVTRRVSKDDWVEWLKRLSIGLLKESPSHALRACRSLAQEYDTLLRDLFNAAFISCWTELSPDLKNELTQSLIQALQVTDMPEITQTILNLAEFMEHCDRDPIPIETKLLGTRAMACRAYAKALRYKEEEFLLREDSQVFESLILINNKLQQREAAEGLLTRYRNAANELNVQGRWYEKLHNWDEALEHYERNLKTDSSDLEARLGHMRCLEALGDWSELSNVTKHEWENFGTEAKSRAGPLAAVAAWGLQDWEAMREYVRCIPEDTQDGSYYRAVLAVHHDDFETAQRLIDETRDLLDTELTSMAGESYERAYGAMVCVQMLAELEEVIQYKLIPERREPLKTMWWKRLQGGQRLVEDWRRIIQVHSLVVKPHEDIHTWLKYASLCRKSGSLHLSHKTLVMLLGTDPKLNPNQPLPCNQPQVTYAYTKYMAANNQLQEAYEQLTHFVSTYSQELSCLPPEALKQQDQRLMARCYLRMATWQNKLQDSIRPDAIQGALECFEKATSYDPNWYKAWHLWAYMNFKVVQAQKSALDKQQPPGASMGMTMGSGLDSDLMIIQRYAVPAVQGFFRSISLIKGNSLQDTLRLLTLWFDYGNHAEVYEALLSGMKLIEINTWLQVIPQLIARIDTHRQLVGQLIHQLLMDIGKNHPQALVYPLTVASKSASLARRNAAFKILDSMRKHSPTLVEQAVMCSEELIRVAILWHEQWHEGLEEASRLYFGDRNVKGMFEILEPLHAMLERGPQTLKETSFSQAYGRELTEAYEWSQRYKTSAVVMDLDRAWDIYYHVFQKISRQLPQLTSLELPYVSPKLMTCKDLELAVPGSYNPGQELIRISIIKTNLQVITSKQRPRKLCIRGSNGKDYMYLLKGHEDLRQDERVMQLFSLVNTLLLDDPDTFRRNLAIQRYAVIPLSTNSGLIGWVPHCDTLHTLIRDYRDKKKVPLNQEHRTMLNFAPDYDHLTLMQKVEVFEHALGQTQGDDLAKLLWLKSPSSELWFERRNNYTRSLAVMSMVGYILGLGDRHPSNLMLDRMSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLIKAMEVTGIEGTYRRTCESVMLVLRRNKDSLMAVLEAFVYDPLLNWRLLDVDKKGNDAVAGAGAPGGRGGSGMQDSLSNSVEDSLPMAKSKPYDPTLQQGGLHNNVADETNSKASQVIKRVKCKLTGTDFQTEKSVNEQSQVELLIQQATNNENLCQCYIGWCPFW

Target ORF information:

RefSeq Version	NM_001273498.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of rapamycin, transcript variant B (Tor), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273498.1

ORF Insert Sequence:

ATGTCGACGA CGTCGGTGGT GCAGCAGTTC GTCAACGGGC TTAAGTCCCG CAACCGGAAT 
GTACAGAACA AGGCAACCCA GGATCTGCTC TTCTATGTGA AGACCGAGCT ACGCGAAATG 
TCCCAGGAGG AGCTGGCCCA ATTTTTCGAC GAGTTCGACC ATCACATTTT CACGATGGTG 
AATGCGACAG ATATCAACGA GAAAAAAGGC GGTGCCCTGG CCATGAAGTG CCTGATTAAC 
TGCGAGGGCA GTCTTACTGC CAGGAAGGGC ATTTCGCCCT ATCTCAACCG CCTGCGCGAT 
CTTCTGCTAA TCAACGATGT GTCCGTCATG GAGATTGCCG CCCGCTCCCT GGTCAAATTG 
GCCAACATGC CCACCTCCAA GGGCGCCGAT TCCTTCGACT TTGATATTAA GAAGGCTTTT 
GAGGTGCTCA GAGGCGAGAG ACAAGAGTAC CGCCGCCATT CAGCCGTCTT TATCCTCCGT 
GAGCTGGCTA TCGCCTTGCC CACATACTTC TACCAGCACA TCCTGACCTT CTTCGAGGTT 
ATCTTTAACG CCATCTTCGA TCCCAAACCT GCTATACGAG AGTCAGCTGG CGAGGCTTTG 
AGGGCGGCTT TGATTGTCAC CGCTCAGCGA GAGAGCACCA AGCAGTCCAG TGAACCACAG 
TGGTACAGAA TCTGCTATGA CGAGGCGAAT GGAAGCTTCA ATGCAGACTT GGGCTCTAGC 
AAGGATCAGA AAGGCGTGAC CCGAGACGAT CGCATTCACG GTGGCCTCGT CGTCTTCAAC 
GAGCTCTTTC GCTGTGCCAA TGCCACCTGG GAGCGGCGCT ACACGTCTCT GAAGACGCTG 
TTCCCCAAGA CCCAACACAA TAAGTTCCTG GAGGCCAGCA GTTCGTCGAG TATGGGTTCC 
CAGCTGAATA CTCTAGTGCC GCGGCTAAAG GTGCCCTTCA TTGACAAGCT CGGCAGCACC 
CAAACGCATT TGGGTGAGGG AGAGCATCAC AAAGGAGTTG CCAAGTTCGC GAGCCAGCAC 
AACGTACTGG AGTCCGCATA TGCTCAGGAG ATCCTTCAGG AGCACTACAC CTCCATTTGC 
GACAATGTTT TGGAGCAGCG AACTTCGAAG TCTCCGTATG TACAGCAGGC ACTCCTTCAA 
ATACTTCCTC GATTGGCTGC CTTTAACCGC GCGGTGTTTG TGGAAAAATA CCTGCAGACA 
TGCGTGTCGC ATTTAATGCA GATCCTGCGA GGCAAGGAGA AGGATCGCAC GGTGGCGTAC 
ATAACCATTG GTTACATGGC TGTAGCGGTG CAGAGTGCGA TCGAAGTTCA TTTATCCAGT 
ATAATGACTA GCGTGAAGGT CGCCTTGCCG TCAAAGGATT TGACCAGCAA ACGAAAGGTA 
CCAGTGGATC CTGCGGTCTT TGCCTGCATA ACGCTTCTTG CTCATGCCGT TAAGTCGGAG 
ATCGCCGACG ATGTTAAGGA TATCCTTGAG CAGATGTTCT ACACGGGTCT CTCGCCAGCT 
CTGACTGTTT GCCTGAGGGA ACTCTCCGAG AATGTGCCGC AGCTTAAGTC GGCCATTACA 
GAAGGATTGA TCGGAATCCT ATCCCAGGTG CTGATGAACA AGGCGGCAAT TTTACCATAC 
ACTGCTTTGC CAACGATCGC CATCGATGGC TCGCTAATGC AGAATGGTGA CGGAGCAACC 
ACAGTGCTGG CACTCAAAAC GCTGGGTACC TTCAACTTTG AGGAGCAAAA CATGCTGGAC 
TTTGTGCAAC GATGTGCTGA CTACTTCATC GTTCACGAGC AGCAGGAGAT TCGTCTGGAG 
GCAGTGCAAA CATGCACACG ACTCCTTAAA CTGGCAGTGC AATCCTCTGA AAGTATGGAG 
AACTCCAAGA CCCTCAGCGA CACCGTTTCC CACGTGATTG AGCGCCTGCT TATGGTAGCC 
ATTACTGATA TGGACTGTAA TGTGCGAATA CGAATTCTAC GTTCGCTGGA CGAGACCTTT 
GATGGGAAGC TTGCTCAGCC AGAGTCTCTA AATTCGCTAT TTATTACGCT GCACGATGAA 
ATCTTTGAGA TCCGGGAGCT GGCAATGGTT ACCATTGGAA GATTGTCGTC CATAAACCCG 
GCTTATGTGA TGCCCAAGCT AAGAACCACC ATGATCGAAT TGATCACGGA TCTAAAGTAC 
TCGGGAATGA GTCGAAACAA GGAGCAGAGC GCAAAGATGC TTGACCATTT GGTGATAAGC 
ACGCCCAGGC TAATATCCTC TTACATGAAT CCGATCCTCA AAGCCTTAGT GCCCAAGTTG 
CATGAGCCGG AATCGAATCC AGGAGTTATT TTAAATGTGC TTCGCACAAT TGGTGATTTA 
GCAGAGGTTA ATGGAGGCTC CGATGAAATG GAATTGTGGG CAGATGACCT TCTGTCCATT 
TTACTCGAAA TGCTGGGCGA CGCTGGCAGT CCGGATAAGC GAGGAGTGGC TCTGTGGACG 
TTGGGACAGT TAATCAGTGC AACAGGCAGG GTGGTTACCC CATATCACAA GTACCCCGTG 
CTCATAGATA TTTTGATTAA TTTCCTTAAA ACGGAACAAC GTCGATCCAT TCGAAGGGAA 
ACGATACGAG TGCTGGGCTT GTTGGGAGCC ATGGATCCTT ACAAGCACAA GATGAACAAG 
GGCTTGATTG ATAGCCAAAA GGATAATGTT TTAATAGCAT ACTCCGATGG CAAAGTCGAT 
GAAAGTCAGG ACATCTCCAC CGCTGAGTTG CTAGTTAACA TGGGTAACGC ATTGGATGAG 
TATTATCCCG CTGTGGCCAT AGCTGCATTG ATGAGGATTC TACGGGATCC CACGTTAAGT 
ACACGTCACA CCAGCGTGGT GCAGGCGGTT ACTTTTATCT TCCAGAGCCT GGGCATCAAA 
TGTGTGCCGT ACTTGGCCCA AGTGCTTCCC AATTTGCTGG ACAATGTACG CACTGCAGAT 
AATAATCTAA GGGAGTTTCT CTTCCAGCAG CTCGCCATTC TGGTGGCTTT TGTCAAGCTG 
CATATCATCA GCTACATGGG AGATATTTTT AAGCTGATCA AAGAATTTTG GACCATTAAT 
ACGCCTCTGC AAAACACTCT AATCAATTTG ATTGAACAGA TCGCCGTGGC TTTGGGATGC 
GAGTTTAGGG ATTATTTGGC GGAGCTGATC CCACAAATTC TGCGAGTGTT GCAGCACGAT 
AACTCCAAGG ATAGAATGGT CACGAGAAGG TTGCTCCAAG CTCTTCAAAA GTTTGGCAGC 
ACTTTGGGTT ACTATCTGCC GCTGATTTTG CCTCCGATCG TGAAACTCTT CGATTCTCCG 
TACGTACCAC AACAGGTGTC AATGGTAGCC CTGGAAACGA TCAACAATCT AGCCTGTCAG 
CTGGACTTCA CCGACTTCTC CTCGCGCATC ATACATCCGT TGGTTCGAGT GCTGGACGCT 
GAGCCAGAGC TTCGGGACCA AGCTATGACA ACCCTTCGCT CACTGGCAAA GCAACTGGGC 
AAGAAGTACC TCGTGTTCGT GCCTATGGTC CAGCGCACTC TCAACAAACA TCGCATTGTG 
GATCCCGAGT ACGAGGAGCT ACTGAGTAAG ATTAAGTCCT GCAGCACACT GGCGGATTCC 
TATGGGGCCG GGGAGTCAGA ACTGCGGCCG TCCAGGTTCA AAAACAACGA GCCATTTGTG 
ACCGACAGAA ATAGCAATAA TAAAAATCTG CAGGTCACCA CAAACGAACT ACGAACGGCG 
TGGCAGGTGA CGCGGCGGGT GTCCAAGGAC GACTGGGTGG AGTGGCTAAA GCGGTTGTCC 
ATTGGCCTGC TGAAGGAATC GCCTTCGCAC GCACTGAGAG CCTGTCGTTC GTTGGCCCAA 
GAGTACGATA CGCTGTTGAG AGATCTTTTT AACGCCGCAT TTATCTCCTG TTGGACGGAA 
CTATCGCCGG ATTTGAAAAA CGAGCTAACA CAGTCACTGA TTCAAGCCCT GCAAGTTACC 
GACATGCCGG AAATCACTCA GACAATCCTC AACTTGGCCG AGTTTATGGA GCATTGCGAT 
CGCGATCCAA TACCAATAGA AACTAAGCTA TTGGGTACTC GAGCGATGGC TTGCAGAGCG 
TATGCCAAGG CATTGCGCTA CAAGGAGGAG GAGTTCCTTC TGCGAGAAGA TTCCCAAGTT 
TTTGAGTCCT TGATTCTAAT CAACAACAAG CTGCAGCAAA GAGAAGCAGC GGAAGGTCTG 
CTCACAAGGT ATCGTAATGC AGCCAACGAA CTAAATGTTC AAGGAAGATG GTATGAAAAA 
CTTCACAATT GGGATGAGGC GCTGGAACAC TATGAAAGGA ATCTAAAGAC GGATTCCTCG 
GACTTGGAGG CGCGACTCGG TCACATGCGC TGCTTGGAGG CCCTCGGAGA TTGGTCGGAG 
CTGAGCAACG TGACCAAGCA CGAGTGGGAA AACTTCGGTA CAGAGGCCAA ATCAAGAGCA 
GGACCCTTGG CCGCAGTGGC CGCCTGGGGT CTCCAGGATT GGGAGGCGAT GCGGGAGTAC 
GTAAGGTGTA TACCAGAGGA TACCCAAGAT GGAAGCTACT ATCGTGCTGT CCTCGCCGTG 
CATCATGACG ACTTTGAGAC CGCACAGCGC CTTATTGACG AAACAAGGGA CCTCCTCGAC 
ACGGAGCTCA CATCAATGGC GGGCGAGTCC TATGAAAGAG CCTACGGAGC CATGGTATGT 
GTACAAATGC TGGCGGAGCT GGAGGAGGTC ATCCAATACA AGCTCATTCC CGAGAGGCGA 
GAGCCGTTAA AGACCATGTG GTGGAAGCGT CTTCAAGGAG GCCAGCGACT AGTAGAAGAC 
TGGCGAAGGA TCATCCAGGT GCACTCGCTG GTGGTAAAAC CGCACGAGGA CATACATACC 
TGGCTGAAGT ACGCTAGTCT GTGCAGGAAG AGCGGCTCCC TGCACTTGTC CCATAAAACT 
CTGGTGATGC TGCTAGGCAC CGATCCCAAG CTTAATCCGA ACCAGCCACT GCCTTGTAAT 
CAACCACAGG TGACCTATGC GTACACCAAG TACATGGCTG CCAATAATCA GCTGCAGGAA 
GCCTACGAGC AGTTGACGCA CTTCGTCAGC ACCTATAGTC AGGAGCTAAG CTGTTTACCT 
CCGGAAGCAC TAAAGCAACA AGATCAGCGA CTCATGGCCC GCTGCTATCT GCGCATGGCC 
ACCTGGCAGA ACAAGCTGCA GGACAGCATC AGACCGGATG CCATTCAAGG CGCACTCGAA 
TGCTTTGAAA AGGCCACCAG CTATGATCCC AACTGGTACA AGGCCTGGCA CCTGTGGGCG 
TACATGAACT TTAAGGTGGT GCAGGCCCAA AAATCGGCGC TGGACAAACA ACAGCCTCCG 
GGAGCCAGCA TGGGAATGAC AATGGGCTCT GGCCTCGACA GTGATCTAAT GATCATACAG 
CGATACGCCG TTCCGGCAGT TCAGGGATTC TTCCGTTCAA TCAGCCTTAT AAAAGGGAAT 
TCGCTGCAGG ATACGCTACG TTTGTTAACT CTTTGGTTTG ACTATGGCAA CCATGCAGAG 
GTGTACGAGG CGCTGCTCTC TGGAATGAAG CTGATCGAGA TCAACACCTG GCTTCAGGTT 
ATCCCGCAGC TTATTGCCCG CATCGATACC CACCGTCAGT TGGTGGGTCA ATTAATCCAC 
CAACTCCTAA TGGACATAGG AAAGAATCAC CCGCAGGCTC TGGTCTATCC TCTAACAGTC 
GCTTCCAAGT CAGCATCATT GGCGCGCAGG AACGCCGCCT TTAAGATCCT CGACTCGATG 
AGGAAACACT CGCCCACCTT GGTAGAGCAG GCGGTCATGT GTAGTGAGGA GCTCATCCGT 
GTGGCGATTC TGTGGCACGA ACAGTGGCAC GAAGGTTTGG AGGAAGCCTC GCGTCTTTAC 
TTTGGAGATC GCAACGTAAA GGGCATGTTT GAGATCTTGG AGCCACTGCA TGCTATGCTG 
GAGAGGGGAC CACAAACACT CAAGGAGACA TCCTTCTCGC AGGCCTATGG ACGCGAGCTG 
ACAGAGGCGT ACGAGTGGTC GCAACGGTAC AAGACATCGG CGGTGGTGAT GGATCTGGAT 
CGAGCGTGGG ACATTTACTA TCACGTCTTC CAAAAGATCT CGCGCCAGCT GCCACAACTT 
ACCTCGCTGG AGCTGCCGTA TGTCTCGCCC AAACTGATGA CGTGCAAAGA TCTCGAACTG 
GCAGTGCCCG GGTCCTACAA TCCTGGCCAG GAGCTCATCC GGATTAGTAT CATTAAAACC 
AACCTTCAGG TGATTACTTC CAAGCAGCGG CCGCGAAAAC TGTGCATACG CGGCTCCAAT 
GGCAAGGACT ATATGTACCT TCTAAAGGGA CACGAGGACC TTCGTCAGGA CGAACGCGTG 
ATGCAGCTAT TCTCCCTGGT GAACACATTG CTGTTGGATG ACCCCGACAC TTTCCGTCGT 
AACTTGGCCA TCCAGCGGTA TGCGGTTATC CCGCTCAGTA CCAACTCGGG TCTTATTGGT 
TGGGTGCCAC ATTGTGACAC CCTTCATACG CTAATCCGGG ACTACCGCGA CAAGAAGAAG 
GTGCCACTGA ATCAGGAGCA TCGCACCATG CTCAATTTTG CGCCAGACTA TGATCACCTC 
ACGCTGATGC AGAAGGTGGA AGTGTTCGAG CATGCGTTGG GTCAGACGCA AGGCGACGAC 
TTGGCCAAGT TGCTGTGGCT CAAGTCTCCG TCCTCTGAAT TGTGGTTTGA ACGGAGAAAC 
AACTATACCC GCTCACTGGC AGTCATGTCA ATGGTTGGAT ATATCCTAGG TCTGGGCGAT 
CGTCATCCAT CGAACCTTAT GCTGGATCGT ATGAGCGGCA AGATACTGCA CATAGACTTT 
GGCGACTGCT TCGAGGTGGC CATGACCCGC GAAAAGTTTC CCGAAAAGAT ACCCTTCCGT 
CTCACCCGGA TGCTAATAAA GGCAATGGAG GTCACTGGCA TCGAGGGCAC CTACAGACGC 
ACCTGCGAAA GCGTGATGCT GGTGCTTCGC CGCAACAAGG ATTCCCTGAT GGCGGTGCTG 
GAGGCCTTTG TCTATGATCC GCTGCTCAAC TGGAGACTGT TGGATGTGGA CAAGAAGGGT 
AACGATGCGG TGGCTGGGGC GGGTGCTCCA GGTGGACGTG GCGGTTCCGG AATGCAGGAT 
AGCCTCAGTA ACTCCGTAGA GGACTCCCTG CCCATGGCCA AGTCGAAGCC CTACGATCCG 
ACGCTACAGC AAGGCGGCCT GCACAACAAC GTGGCCGACG AGACGAACAG CAAAGCCAGC 
CAGGTAATCA AGCGGGTCAA GTGCAAGCTA ACAGGCACCG ACTTCCAGAC GGAAAAGAGC 
GTAAACGAGC AGTCGCAGGT GGAGCTGCTC ATCCAGCAGG CGACCAATAA CGAGAACCTC 
TGCCAGTGCT ACATTGGCTG GTGTCCGTTT TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tor cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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