repo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	repo
Entrez Gene ID	47285
Full Name	reversed polarity
Synonyms	3702,AbRK2,CG31240,CG8045(CT24072),CT24072,Dmel\CG31240,REPO,RK2,Repo,l(3)03702,rk2
General protein information	Preferred Names reversed polarity Names CG31240 gene product from transcript CG31240-RA CG31240-PA antibody RK2 repo-PA reverse polarity
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90F9-90F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_057678.5	NP_477026.1	reversed polarity

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

REPO directly regulates DRPR expression. gcm and repo have a critical role in controlling glial phagocytic function through regulation of SIMU and DRPR specific expression.
Title: Developmental regulation of glial cell phagocytic function during Drosophila embryogenesis.

we characterized three cis-regulatory elements
Title: Characterization of cis-regulatory elements controlling repo transcription in Drosophila melanogaster.

These results indicate that cas could be directly suppressed by Repo.
Title: Ectopic Repo suppresses expression of castor gene in Drosophila central nervous system.

novel role for the dVDUP1 tumor suppressor during nervous system development as a regulatory target for REPO during gliogenesis
Title: The tumor suppressor, vitamin D3 up-regulated protein 1 (VDUP1), functions downstream of REPO during Drosophila gliogenesis.

Gcm acts synergistically with other factors to control repo transcription in glial cells
Title: Transcriptional regulation of the Drosophila glial gene repo.

The following repo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the repo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08872	NM_057678.5 Latest version!	Drosophila melanogaster reversed polarity (repo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

repo ( NM_057678.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477026.1 Drosophila melanogaster reversed polarity (repo), mRNA.

Price & Availability↑

CloneID	OFa08872
Clone ID Related Accession (Same CDS sequence)	NM_057678.5
Accession Version	NM_057678.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	reversed polarity
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057678.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAACACG ATAGCTTCGA TGATCCCATT TTCGGGGAGT TCGGCGGCGG GCCACTCAAT 
CCACTGGGCG CCAAGCCACT GATGCCCACC ACAACGGCCA TGCATCCCGT GATGCTGGGC 
AGTGTCCACG AGTTGTGCAG CCAACAGCAG CAGCAGCAGC AGCAGCAACG ATTGCCCGAC 
TGCAACACCA TACTGCCGAA TGGCGGAGGA GGAGGAGCAG GATCTGGCGG CGCTGGAGGA 
TCGCCCAACT ATGTGACCAA GCTGGATTTT GTCAACAAGA TGGGCTGCTA CTCGCCCAGC 
CAGAAGTACG AGTACATTAG TGCGCCGCAA AAACTGGTGG AGCAGCAGCA GCAGCAGCAG 
CATCATCATC AACATCATCA GCACTACACG GCCACGCCCC CGCCCAGCCA CAATGGCATC 
CTGCTGCACA AGCAATCCTC CCAGCAGCAA CAGCAGCAGC AGCAACAGCT GGTGGGCATC 
CTGGACTATC ATCCGCTCAA TGGCAAGCTG GACTACTCCT CGTCGCCCAA AGATCAGTAC 
GAGCCGCAGC AGCTGCAGCA TCTGTACGGC GGCAGTCCGC ATCATCTGGA CCATTTGGAT 
CATGGCAGTG ATGGCTTGCT CCAGGACTCC TCGCCCGTCA TGATCAACGG AGGATCCGCT 
GGTGGCAAGC TAAAGAAGCC CGATGAGATG TGTTCGCAGT TGGAGGCGGG TGGTGCTGGA 
GTTACTCCGC CAAGTAGTTC CTCCACGGTG GTTAATGGCA CCACCAATGG CACTGGCAAC 
GCCAACTCCT CCTCCTCCGC TGGCGTCGGC ATTCAGGGGG CGGCGGGCAC AGCGGGGGGC 
GTGGCAGCTC CTGCAGCCAA AAAGGACGGA AGCAGCAGCA AGAAGAAGGG CGATCCCAAT 
GGCATCAAGA AGAAGAAGAC GAGAACCACC TTTACTGCCT ACCAGCTGGA GGAGTTGGAG 
CGTGCCTTTG AACGTGCTCC GTATCCGGAT GTTTTCGCCC GCGAGGAGCT GGCCATCAAG 
CTGAATCTGA GCGAATCCCG TGTGCAGGTG TGGTTCCAGA ACCGACGGGC CAAGTGGCGC 
AAGCACGAGC CACCGCGCAA GACGGGCTAC ATCAAGACGA GCACTCCGCC GACGGCGACA 
CTGAATCCCG GAACCCTAGC CCCACCATTC ACTAGCTACC CGCAGACGAC GACGGTCACG 
CCGCCGGGCA GCATGGACAG CTGGACCAGC TACCAGACGC CCTACGAACT GACGCCCCAG 
TTCAGCCTGC TCTCGCCGGC GGCCAGTCCG TATGGCACCT ATTCCGGCCA GTACGGTGCC 
TATGTGCACG AAAGCCAGCT CTTCCCGATG CGTCACTACG AGTACGGTAG TCCCACGCGC 
ATGGAAATGG GCGCCACAAC CGGTTCGGTG GCCGGAAACG GAGATGAGTC GGTGGCCAAT 
GGCGGCAGCT ATCAGACGGC GGAACTGCAG ACCGCCCAGC AGCAGCAACT GGCCGACGGC 
ACACTGGTCA CCGTCCATGC CCACCAACAG CAGCAGCAGC AGCAGCAGCA ACTCAATGGC 
AAGTACCTGA GCGCCGAGGA GGCCAAATAC GTGCACCTGC AGTGCCATCA GAGTGGAGGT 
GGTCTGGAGC TAAGTCCCGG CGCCAGCTGT CATCTGGTGG AGGCGCAGGG CCAGCACTAT 
GTGACCACGG CGACGGGAGG AGCTGCATCC GCGGGCGGCA CCAGCAGCGA TGACAACGAC 
AGCGGCGGCA TGCAGACTGT GATCAAGAGC GAGGAGGTCT CCCAACAGCA GCAGCAGCAG 
CAGGGACAGA GCTACGTCCT GCCGCCTTTT TTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477026.1
CDS	306..2144
Translation	MEHDSFDDPIFGEFGGGPLNPLGAKPLMPTTTAMHPVMLGSVHELCSQQQQQQQQQRLPDCNTILPNGGGGGAGSGGAGGSPNYVTKLDFVNKMGCYSPSQKYEYISAPQKLVEQQQQQQHHHQHHQHYTATPPPSHNGILLHKQSSQQQQQQQQQLVGILDYHPLNGKLDYSSSPKDQYEPQQLQHLYGGSPHHLDHLDHGSDGLLQDSSPVMINGGSAGGKLKKPDEMCSQLEAGGAGVTPPSSSSTVVNGTTNGTGNANSSSSAGVGIQGAAGTAGGVAAPAAKKDGSSSKKKGDPNGIKKKKTRTTFTAYQLEELERAFERAPYPDVFAREELAIKLNLSESRVQVWFQNRRAKWRKHEPPRKTGYIKTSTPPTATLNPGTLAPPFTSYPQTTTVTPPGSMDSWTSYQTPYELTPQFSLLSPAASPYGTYSGQYGAYVHESQLFPMRHYEYGSPTRMEMGATTGSVAGNGDESVANGGSYQTAELQTAQQQQLADGTLVTVHAHQQQQQQQQQLNGKYLSAEEAKYVHLQCHQSGGGLELSPGASCHLVEAQGQHYVTTATGGAASAGGTSSDDNDSGGMQTVIKSEEVSQQQQQQQGQSYVLPPFLH

Target ORF information:

RefSeq Version	NM_057678.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster reversed polarity (repo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057678.5

ORF Insert Sequence:

ATGGAACACG ATAGCTTCGA TGATCCCATT TTCGGGGAGT TCGGCGGCGG GCCACTCAAT 
CCACTGGGCG CCAAGCCACT GATGCCCACC ACAACGGCCA TGCATCCCGT GATGCTGGGC 
AGTGTCCACG AGTTGTGCAG CCAACAGCAG CAGCAGCAGC AGCAGCAACG ATTGCCCGAC 
TGCAACACCA TACTGCCGAA TGGCGGAGGA GGAGGAGCAG GATCTGGCGG CGCTGGAGGA 
TCGCCCAACT ATGTGACCAA GCTGGATTTT GTCAACAAGA TGGGCTGCTA CTCGCCCAGC 
CAGAAGTACG AGTACATTAG TGCGCCGCAA AAACTGGTGG AGCAGCAGCA GCAGCAGCAG 
CATCATCATC AACATCATCA GCACTACACG GCCACGCCCC CGCCCAGCCA CAATGGCATC 
CTGCTGCACA AGCAATCCTC CCAGCAGCAA CAGCAGCAGC AGCAACAGCT GGTGGGCATC 
CTGGACTATC ATCCGCTCAA TGGCAAGCTG GACTACTCCT CGTCGCCCAA AGATCAGTAC 
GAGCCGCAGC AGCTGCAGCA TCTGTACGGC GGCAGTCCGC ATCATCTGGA CCATTTGGAT 
CATGGCAGTG ATGGCTTGCT CCAGGACTCC TCGCCCGTCA TGATCAACGG AGGATCCGCT 
GGTGGCAAGC TAAAGAAGCC CGATGAGATG TGTTCGCAGT TGGAGGCGGG TGGTGCTGGA 
GTTACTCCGC CAAGTAGTTC CTCCACGGTG GTTAATGGCA CCACCAATGG CACTGGCAAC 
GCCAACTCCT CCTCCTCCGC TGGCGTCGGC ATTCAGGGGG CGGCGGGCAC AGCGGGGGGC 
GTGGCAGCTC CTGCAGCCAA AAAGGACGGA AGCAGCAGCA AGAAGAAGGG CGATCCCAAT 
GGCATCAAGA AGAAGAAGAC GAGAACCACC TTTACTGCCT ACCAGCTGGA GGAGTTGGAG 
CGTGCCTTTG AACGTGCTCC GTATCCGGAT GTTTTCGCCC GCGAGGAGCT GGCCATCAAG 
CTGAATCTGA GCGAATCCCG TGTGCAGGTG TGGTTCCAGA ACCGACGGGC CAAGTGGCGC 
AAGCACGAGC CACCGCGCAA GACGGGCTAC ATCAAGACGA GCACTCCGCC GACGGCGACA 
CTGAATCCCG GAACCCTAGC CCCACCATTC ACTAGCTACC CGCAGACGAC GACGGTCACG 
CCGCCGGGCA GCATGGACAG CTGGACCAGC TACCAGACGC CCTACGAACT GACGCCCCAG 
TTCAGCCTGC TCTCGCCGGC GGCCAGTCCG TATGGCACCT ATTCCGGCCA GTACGGTGCC 
TATGTGCACG AAAGCCAGCT CTTCCCGATG CGTCACTACG AGTACGGTAG TCCCACGCGC 
ATGGAAATGG GCGCCACAAC CGGTTCGGTG GCCGGAAACG GAGATGAGTC GGTGGCCAAT 
GGCGGCAGCT ATCAGACGGC GGAACTGCAG ACCGCCCAGC AGCAGCAACT GGCCGACGGC 
ACACTGGTCA CCGTCCATGC CCACCAACAG CAGCAGCAGC AGCAGCAGCA ACTCAATGGC 
AAGTACCTGA GCGCCGAGGA GGCCAAATAC GTGCACCTGC AGTGCCATCA GAGTGGAGGT 
GGTCTGGAGC TAAGTCCCGG CGCCAGCTGT CATCTGGTGG AGGCGCAGGG CCAGCACTAT 
GTGACCACGG CGACGGGAGG AGCTGCATCC GCGGGCGGCA CCAGCAGCGA TGACAACGAC 
AGCGGCGGCA TGCAGACTGT GATCAAGAGC GAGGAGGTCT CCCAACAGCA GCAGCAGCAG 
CAGGGACAGA GCTACGTCCT GCCGCCTTTT TTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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repo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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