ebo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ebo
Entrez Gene ID	47141
Full Name	ellipsoid body open
Synonyms	CG3923,Dmel\CG3923,EBO,EG:165H7.3,Exp1,Exp6
General protein information	Preferred Names ellipsoid body open Names CG3923 gene product from transcript CG3923-RA CG3923-PA CG3923-PB CG3923-PC Exportin-6 ebo-PA ebo-PB ebo-PC ellipsoid-body-open exportin 6
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1B4-1B4\|1-3.8 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297809.1	NP_001284738.1	ellipsoid body open, isoform B
NM_080140.3	NP_524879.2	ellipsoid body open, isoform A
NM_001297810.1	NP_001284739.1	ellipsoid body open, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Exp6	NP_524879.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000271	XP_566117.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ebo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ebo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02408	NM_080140.3 Latest version!	Drosophila melanogaster ellipsoid body open (ebo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa22261	NM_001297810.1 Latest version!	Drosophila melanogaster ellipsoid body open (ebo), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa22262	NM_001297809.1 Latest version!	Drosophila melanogaster ellipsoid body open (ebo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ebo ( NM_080140.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524879.2 Drosophila melanogaster ellipsoid body open (ebo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02408
Clone ID Related Accession (Same CDS sequence)	NM_080140.3
Accession Version	NM_080140.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ellipsoid body open, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080140.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGCCG CATCTTTGAC GACGGTGGAG GGACTGCTGC AGGAGTTTTA TCAGCCCAGC 
ACCTCCAATG CCCGGAAACG AGAGATCGAG ACAAACCTCT TGGCCTTCAA GTCCCAGCCA 
GAGGCGTGGC AACTTTGCCT CCGTGTGGCC ACCTCTTCCG ACACCACGGA GAATCAGTTC 
CTCTGGTTCT TCAGCACCTC AACCTTGGAG CACACAATCA CGCGGAGATG GACACAGTTG 
ACGTCCACGG ACAAGACTTT GCTGCGGGAG ACCCTTTGGA ACTCGTATGC CCAACTGGTA 
GCCACTCCAA ATGTGGCCAA ACGGCACAGG GACACACTGG CCCAGTTGAT AGCCCTACTC 
GGAAAGAGGG AGTTCCCTGA ACAGGACCCC AATTACATGC AACACTGCAT GGAACTGACA 
AAAACACGGT TTCAACTTGG CATCAACCTG CTTAAGATCA CCTCCGAGGA GGTGGTAAGC 
AACAGGGGCG ACCTGACCAC CGAATGGAAG CAGTACTTCT ACTCCTGCAT CTCCATGTGT 
GTACCCGATG TTCTGGACCT GGTAACCAAG TATCTGCTAA TCGCTGTATG CCACATCAAT 
GGCAAAGACA TCCAAACAAC CATACCAAAC ACCCTCATGG ACTTTAGTCT AACCTCAGCG 
CTGCCAAATG ACAATCAATT AAGTTCCTCA ATTTTGGACC TGCTAGGATG TGTGCAGCAC 
TTGGTCTCCT GGATACGCAC TGAACTGATC TCGGAGTACT TCCTTATGAG CATACTTGAC 
CTTTCCCAGT GGCGACCAGC CCATGAGCCC ATCTCCTTGG CGGCTCTCTC TGTGTTGAAT 
GAGCTCCTCT ACCTCCAGAA GCCACTGCCC TTCCCAGGAC CCTTGATGGG CGGCGTTACC 
AGCCTCCTGG AGCAGCACAA TAACAATCGG CGGCAGAGCG AGATGTACAG CGACAAGTTT 
CGGGAGCTGC TGCGCCTCTA CACCACCAAG TATGCCGCCA AGCTGATGCA GGAGCCAGAC 
CTGCTGGAGA CCTTTCTCAA TCTGCTATAC AGCTGCACCA CTGAATTACA CGGCGCCTTG 
GACTTCACCG AAAAGCTTGA GATCTGGACA CCCATCATCA AAGGCATTGC CCAGCACCCC 
GCCAAAATCA CGCGTTATAA CAATGTGCTT ACCCAGCTAG TAGATGAGAT TATGAGGCGC 
ACTCAGTTTG AGGCCAACAA ACCCGAGCTA GAAGTGCTGG ACAACGAGCT CATGGAGGAC 
GATACCTCCG CCACTGAGTG GCAGCAGTTT CTAGACCAAT GCTTCGAGTC CCTGGCCCTA 
CTGGCCAACA CCAGAGGTGC ACACATCGTT TTCGCACAGG TCTATAACCA CTGGTCACGG 
CCACAGATGT ATTTACTGTC CTTGGAGCAC GCATTGGACC ACGGAAGCGC TCGAAGCTAC 
GAAGCGGCAC GTAAACTGAA GGGCGCCAAT GTGGGCGAGA TTTTGCGCGA CTTTGCCACC 
GTCTGCCAGG CGGTCGTGCG TTTGGCCCCA TTGATGGACT CATCAACAGC AGCACCCGGG 
GTTGCTGATG AGATGGAGTG CCAGCTGCAG ATGCTGTCCG ACAGTTTGCT GCAGACTCTA 
CAGCTCCTCG CAAGCAATCG TTTGTATAGC GGCGGCAGCG AAATGGACAA AGCTACTTTC 
CAGACTGATT TGGACAACCT ATACGCTCAA GTTCTACTGG CCATGAGGAG CATCTTTCCG 
CTAACGAAAG CCATGAGCGG CGAGGAACTG CTGCACCGCC TGTTTGCTAT CCTGGGCAGC 
ATTTTCGCAT GCAATGGATC CTTAACGGCT GCCTCCCCCA TTGTGCAGCA GGCGGCAAGC 
GAGCTGCTGC TTTTCATATC GACTGTGATA CGGCCCAAGT GCCTGCTGGA TATACCACAA 
ATCCAAAGCC TGCTGCAGGC AGGCTCTCGC TTGGGCGCCC AATTGCCGCG TCAGGTGTGC 
ATCAATGTAC ACATCAGTCT GGTCAGCTAC ATGGTGCTGC CGTGGAAAAG CGTTCCGGAG 
CAGCAGCAGG AGTATCCGAG GCGTCTCTGG ATGCTGCGTG AATATGTCCA GGGGCTGGCA 
CAGAACTTTC TGGATCTGGA CTTAGGCCAG GCCAACGAGA GCAAGGTGGC GGCCACCACG 
CTCAGTTTGC TGGGTACTTT CACTGCTCTC ATTGAGTATT TTAAAGCTAC TGGAGGCAAT 
GCCAAGGATA TGCTGGCAAG CACTTTCAAG CCTATTTTAA CAAAGGCCCT AATGATTTTT 
AACAACTTTG GGCAAAGCAG CATCGCCATG GCCATCACGG TGGCCGAGTT TAGCCTGAGC 
ATGCTCCGAA CATTGCCCAC CCATTTGGGG GCCCAGTTCA TTAAGGAAAT GATCACCTTG 
TTCATAAGTG TCAGCAGTAG GGAGCAACTG ACTCTGAGCC GACTGGCTGT GATGGAAAAA 
ATACTGCAGA TGTTCAAGTT GATCGTGGAG CAGCCAGGGA GCGCCTCCTT GGGGCTGCTT 
CCCTCCATTT TAGATTTTAG CACTCAGCAG ATTCTGCCCT TGTTGCAGCA GCAAAATGCG 
GCAACCGATA ACAGCGAGAT CACTAGCCTG TTGTACGCTT TGTTTGATAG TGTCCTCACC 
TGCAAGTGGC AGTTCTTCTA CAAAAATCAG CTCTCCAATG GTCACAGCGG TGGTGGCTCC 
GGCAACTTCA ACTGCAGCGA CAACCTGCAT CCAGCACACT TCATGGCCAT CCTGAATGCG 
TATGGCCAGA TGCTGGTCAG TGGGACTGAT CCGAGCAATG TGCGCAGCGT TCTCTTCTCA 
ATGCAAAATG TGAATGAGAG GAGTCGGTTG TACCAACGCG CCTTGTTCAA GGAGCAACTG 
CTGGCTTCAT TTCAGCGGGC CTTGCTCAAT CTGCTGTTGA GTGGAGAGGG CTCCCTCCAC 
TTCGACATGA TTGCCCAGGC GTTGTATGCT ATGGGCCAGG TGGACCGACG CCAGTTGCTC 
GAGAGCTTCG TTCACGCCTC TTTGCCAGTG GCTCAGACGT CGCTGGAGGA GATCTGCCAG 
ACAAATGACA TTCCAACGTT TACGCAGAAA CTCACGCAGC TCATGCAGGA CGCCCACTGC 
GTGCACCTCA ATGAGACGGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524879.2
CDS	184..3327
Translation	MHAASLTTVEGLLQEFYQPSTSNARKREIETNLLAFKSQPEAWQLCLRVATSSDTTENQFLWFFSTSTLEHTITRRWTQLTSTDKTLLRETLWNSYAQLVATPNVAKRHRDTLAQLIALLGKREFPEQDPNYMQHCMELTKTRFQLGINLLKITSEEVVSNRGDLTTEWKQYFYSCISMCVPDVLDLVTKYLLIAVCHINGKDIQTTIPNTLMDFSLTSALPNDNQLSSSILDLLGCVQHLVSWIRTELISEYFLMSILDLSQWRPAHEPISLAALSVLNELLYLQKPLPFPGPLMGGVTSLLEQHNNNRRQSEMYSDKFRELLRLYTTKYAAKLMQEPDLLETFLNLLYSCTTELHGALDFTEKLEIWTPIIKGIAQHPAKITRYNNVLTQLVDEIMRRTQFEANKPELEVLDNELMEDDTSATEWQQFLDQCFESLALLANTRGAHIVFAQVYNHWSRPQMYLLSLEHALDHGSARSYEAARKLKGANVGEILRDFATVCQAVVRLAPLMDSSTAAPGVADEMECQLQMLSDSLLQTLQLLASNRLYSGGSEMDKATFQTDLDNLYAQVLLAMRSIFPLTKAMSGEELLHRLFAILGSIFACNGSLTAASPIVQQAASELLLFISTVIRPKCLLDIPQIQSLLQAGSRLGAQLPRQVCINVHISLVSYMVLPWKSVPEQQQEYPRRLWMLREYVQGLAQNFLDLDLGQANESKVAATTLSLLGTFTALIEYFKATGGNAKDMLASTFKPILTKALMIFNNFGQSSIAMAITVAEFSLSMLRTLPTHLGAQFIKEMITLFISVSSREQLTLSRLAVMEKILQMFKLIVEQPGSASLGLLPSILDFSTQQILPLLQQQNAATDNSEITSLLYALFDSVLTCKWQFFYKNQLSNGHSGGGSGNFNCSDNLHPAHFMAILNAYGQMLVSGTDPSNVRSVLFSMQNVNERSRLYQRALFKEQLLASFQRALLNLLLSGEGSLHFDMIAQALYAMGQVDRRQLLESFVHASLPVAQTSLEEICQTNDIPTFTQKLTQLMQDAHCVHLNETA

Target ORF information:

RefSeq Version	NM_080140.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ellipsoid body open (ebo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080140.3

ORF Insert Sequence:

ATGCACGCCG CATCTTTGAC GACGGTGGAG GGACTGCTGC AGGAGTTTTA TCAGCCCAGC 
ACCTCCAATG CCCGGAAACG AGAGATCGAG ACAAACCTCT TGGCCTTCAA GTCCCAGCCA 
GAGGCGTGGC AACTTTGCCT CCGTGTGGCC ACCTCTTCCG ACACCACGGA GAATCAGTTC 
CTCTGGTTCT TCAGCACCTC AACCTTGGAG CACACAATCA CGCGGAGATG GACACAGTTG 
ACGTCCACGG ACAAGACTTT GCTGCGGGAG ACCCTTTGGA ACTCGTATGC CCAACTGGTA 
GCCACTCCAA ATGTGGCCAA ACGGCACAGG GACACACTGG CCCAGTTGAT AGCCCTACTC 
GGAAAGAGGG AGTTCCCTGA ACAGGACCCC AATTACATGC AACACTGCAT GGAACTGACA 
AAAACACGGT TTCAACTTGG CATCAACCTG CTTAAGATCA CCTCCGAGGA GGTGGTAAGC 
AACAGGGGCG ACCTGACCAC CGAATGGAAG CAGTACTTCT ACTCCTGCAT CTCCATGTGT 
GTACCCGATG TTCTGGACCT GGTAACCAAG TATCTGCTAA TCGCTGTATG CCACATCAAT 
GGCAAAGACA TCCAAACAAC CATACCAAAC ACCCTCATGG ACTTTAGTCT AACCTCAGCG 
CTGCCAAATG ACAATCAATT AAGTTCCTCA ATTTTGGACC TGCTAGGATG TGTGCAGCAC 
TTGGTCTCCT GGATACGCAC TGAACTGATC TCGGAGTACT TCCTTATGAG CATACTTGAC 
CTTTCCCAGT GGCGACCAGC CCATGAGCCC ATCTCCTTGG CGGCTCTCTC TGTGTTGAAT 
GAGCTCCTCT ACCTCCAGAA GCCACTGCCC TTCCCAGGAC CCTTGATGGG CGGCGTTACC 
AGCCTCCTGG AGCAGCACAA TAACAATCGG CGGCAGAGCG AGATGTACAG CGACAAGTTT 
CGGGAGCTGC TGCGCCTCTA CACCACCAAG TATGCCGCCA AGCTGATGCA GGAGCCAGAC 
CTGCTGGAGA CCTTTCTCAA TCTGCTATAC AGCTGCACCA CTGAATTACA CGGCGCCTTG 
GACTTCACCG AAAAGCTTGA GATCTGGACA CCCATCATCA AAGGCATTGC CCAGCACCCC 
GCCAAAATCA CGCGTTATAA CAATGTGCTT ACCCAGCTAG TAGATGAGAT TATGAGGCGC 
ACTCAGTTTG AGGCCAACAA ACCCGAGCTA GAAGTGCTGG ACAACGAGCT CATGGAGGAC 
GATACCTCCG CCACTGAGTG GCAGCAGTTT CTAGACCAAT GCTTCGAGTC CCTGGCCCTA 
CTGGCCAACA CCAGAGGTGC ACACATCGTT TTCGCACAGG TCTATAACCA CTGGTCACGG 
CCACAGATGT ATTTACTGTC CTTGGAGCAC GCATTGGACC ACGGAAGCGC TCGAAGCTAC 
GAAGCGGCAC GTAAACTGAA GGGCGCCAAT GTGGGCGAGA TTTTGCGCGA CTTTGCCACC 
GTCTGCCAGG CGGTCGTGCG TTTGGCCCCA TTGATGGACT CATCAACAGC AGCACCCGGG 
GTTGCTGATG AGATGGAGTG CCAGCTGCAG ATGCTGTCCG ACAGTTTGCT GCAGACTCTA 
CAGCTCCTCG CAAGCAATCG TTTGTATAGC GGCGGCAGCG AAATGGACAA AGCTACTTTC 
CAGACTGATT TGGACAACCT ATACGCTCAA GTTCTACTGG CCATGAGGAG CATCTTTCCG 
CTAACGAAAG CCATGAGCGG CGAGGAACTG CTGCACCGCC TGTTTGCTAT CCTGGGCAGC 
ATTTTCGCAT GCAATGGATC CTTAACGGCT GCCTCCCCCA TTGTGCAGCA GGCGGCAAGC 
GAGCTGCTGC TTTTCATATC GACTGTGATA CGGCCCAAGT GCCTGCTGGA TATACCACAA 
ATCCAAAGCC TGCTGCAGGC AGGCTCTCGC TTGGGCGCCC AATTGCCGCG TCAGGTGTGC 
ATCAATGTAC ACATCAGTCT GGTCAGCTAC ATGGTGCTGC CGTGGAAAAG CGTTCCGGAG 
CAGCAGCAGG AGTATCCGAG GCGTCTCTGG ATGCTGCGTG AATATGTCCA GGGGCTGGCA 
CAGAACTTTC TGGATCTGGA CTTAGGCCAG GCCAACGAGA GCAAGGTGGC GGCCACCACG 
CTCAGTTTGC TGGGTACTTT CACTGCTCTC ATTGAGTATT TTAAAGCTAC TGGAGGCAAT 
GCCAAGGATA TGCTGGCAAG CACTTTCAAG CCTATTTTAA CAAAGGCCCT AATGATTTTT 
AACAACTTTG GGCAAAGCAG CATCGCCATG GCCATCACGG TGGCCGAGTT TAGCCTGAGC 
ATGCTCCGAA CATTGCCCAC CCATTTGGGG GCCCAGTTCA TTAAGGAAAT GATCACCTTG 
TTCATAAGTG TCAGCAGTAG GGAGCAACTG ACTCTGAGCC GACTGGCTGT GATGGAAAAA 
ATACTGCAGA TGTTCAAGTT GATCGTGGAG CAGCCAGGGA GCGCCTCCTT GGGGCTGCTT 
CCCTCCATTT TAGATTTTAG CACTCAGCAG ATTCTGCCCT TGTTGCAGCA GCAAAATGCG 
GCAACCGATA ACAGCGAGAT CACTAGCCTG TTGTACGCTT TGTTTGATAG TGTCCTCACC 
TGCAAGTGGC AGTTCTTCTA CAAAAATCAG CTCTCCAATG GTCACAGCGG TGGTGGCTCC 
GGCAACTTCA ACTGCAGCGA CAACCTGCAT CCAGCACACT TCATGGCCAT CCTGAATGCG 
TATGGCCAGA TGCTGGTCAG TGGGACTGAT CCGAGCAATG TGCGCAGCGT TCTCTTCTCA 
ATGCAAAATG TGAATGAGAG GAGTCGGTTG TACCAACGCG CCTTGTTCAA GGAGCAACTG 
CTGGCTTCAT TTCAGCGGGC CTTGCTCAAT CTGCTGTTGA GTGGAGAGGG CTCCCTCCAC 
TTCGACATGA TTGCCCAGGC GTTGTATGCT ATGGGCCAGG TGGACCGACG CCAGTTGCTC 
GAGAGCTTCG TTCACGCCTC TTTGCCAGTG GCTCAGACGT CGCTGGAGGA GATCTGCCAG 
ACAAATGACA TTCCAACGTT TACGCAGAAA CTCACGCAGC TCATGCAGGA CGCCCACTGC 
GTGCACCTCA ATGAGACGGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ebo ( NM_001297810.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284739.1 Drosophila melanogaster ellipsoid body open (ebo), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa22261
Clone ID Related Accession (Same CDS sequence)	NM_001297810.1
Accession Version	NM_001297810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ellipsoid body open, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGCCG CATCTTTGAC GACGGTGGAG GGACTGCTGC AGGAGTTTTA TCAGCCCAGC 
ACCTCCAATG CCCGGAAACG AGAGATCGAG ACAAACCTCT TGGCCTTCAA GTCCCAGCCA 
GAGGCGTGGC AACTTTGCCT CCGTGTGGCC ACCTCTTCCG ACACCACGGA GAATCAGTTC 
CTCTGGTTCT TCAGCACCTC AACCTTGGAG CACACAATCA CGCGGAGATG GACACAGTTG 
ACGTCCACGG ACAAGACTTT GCTGCGGGAG ACCCTTTGGA ACTCGTATGC CCAACTGGTA 
GCCACTCCAA ATGTGGCCAA ACGGCACAGG GACACACTGG CCCAGTTGAT AGCCCTACTC 
GGAAAGAGGG AGTTCCCTGA ACAGGACCCC AATTACATGC AACACTGCAT GGAACTGACA 
AAAACACGGT TTCAACTTGG CATCAACCTG CTTAAGATCA CCTCCGAGGA GGTGGTAAGC 
AACAGGGGCG ACCTGACCAC CGAATGGAAG CAGTACTTCT ACTCCTGCAT CTCCATGTGT 
GTACCCGATG TTCTGGACCT GGTAACCAAG TATCTGCTAA TCGCTGTATG CCACATCAAT 
GGCAAAGACA TCCAAACAAC CATACCAAAC ACCCTCATGG ACTTTAGTCT AACCTCAGCG 
CTGCCAAATG ACAATCAATT AAGTTCCTCA ATTTTGGACC TGCTAGGATG TGTGCAGCAC 
TTGGTCTCCT GGATACGCAC TGAACTGATC TCGGAGTACT TCCTTATGAG CATACTTGAC 
CTTTCCCAGT GGCGACCAGC CCATGAGCCC ATCTCCTTGG CGGCTCTCTC TGTGTTGAAT 
GAGCTCCTCT ACCTCCAGAA GCCACTGCCC TTCCCAGGAC CCTTGATGGG CGGCGTTACC 
AGCCTCCTGG AGCAGCACAA TAACAATCGG CGGCAGAGCG AGATGTACAG CGACAAGTTT 
CGGGAGCTGC TGCGCCTCTA CACCACCAAG TATGCCGCCA AGCTGATGCA GGAGCCAGAC 
CTGCTGGAGA CCTTTCTCAA TCTGCTATAC AGCTGCACCA CTGAATTACA CGGCGCCTTG 
GACTTCACCG AAAAGCTTGA GATCTGGACA CCCATCATCA AAGGCATTGC CCAGCACCCC 
GCCAAAATCA CGCGTTATAA CAATGTGCTT ACCCAGCTAG TAGATGAGAT TATGAGGCGC 
ACTCAGTTTG AGGCCAACAA ACCCGAGCTA GAAGTGCTGG ACAACGAGCT CATGGAGGAC 
GATACCTCCG CCACTGAGTG GCAGCAGTTT CTAGACCAAT GCTTCGAGTC CCTGGCCCTA 
CTGGCCAACA CCAGAGGTGC ACACATCGTT TTCGCACAGG TCTATAACCA CTGGTCACGG 
CCACAGATGT ATTTACTGTC CTTGGAGCAC GCATTGGACC ACGGAAGCGC TCGAAGCTAC 
GAAGCGGCAC GTAAACTGAA GGGCGCCAAT GTGGGCGAGA TTTTGCGCGA CTTTGCCACC 
GTCTGCCAGG CGGTCGTGCG TTTGGCCCCA TTGATGGACT CATCAACAGC AGCACCCGGG 
GTTGCTGATG AGATGGAGTG CCAGCTGCAG ATGCTGTCCG ACAGTTTGCT GCAGACTCTA 
CAGCTCCTCG CAAGCAATCG TTTGTATAGC GGCGGCAGCG AAATGGACAA AGCTACTTTC 
CAGACTGATT TGGACAACCT ATACGCTCAA GTTCTACTGG CCATGAGGAG CATCTTTCCG 
CTAACGAAAG CCATGAGCGG CGAGGAACTG CTGCACCGCC TGTTTGCTAT CCTGGGCAGC 
ATTTTCGCAT GCAATGGATC CTTAACGGCT GCCTCCCCCA TTGTGCAGCA GGCGGCAAGC 
GAGCTGCTGC TTTTCATATC GACTGTGATA CGGCCCAAGT GCCTGCTGGA TATACCACAA 
ATCCAAAGCC TGCTGCAGGC AGGCTCTCGC TTGGGCGCCC AATTGCCGCG TCAGGTGTGC 
ATCAATGTAC ACATCAGTCT GGTCAGCTAC ATGGTGCTGC CGTGGAAAAG CGTTCCGGAG 
CAGCAGCAGG AGTATCCGAG GCGTCTCTGG ATGCTGCGTG AATATGTCCA GGGGCTGGCA 
CAGAACTTTC TGGATCTGGA CTTAGGCCAG GCCAACGAGA GCAAGGTGGC GGCCACCACG 
CTCAGTTTGC TGGGTACTTT CACTGCTCTC ATTGAGTATT TTAAAGCTAC TGGAGGCAAT 
GCCAAGGATA TGCTGGCAAG CACTTTCAAG CCTATTTTAA CAAAGGCCCT AATGATTTTT 
AACAACTTTG GGCAAAGCAG CATCGCCATG GCCATCACGG TGGCCGAGTT TAGCCTGAGC 
ATGCTCCGAA CATTGCCCAC CCATTTGGGG GCCCAGTTCA TTAAGGAAAT GATCACCTTG 
TTCATAAGTG TCAGCAGTAG GGAGCAACTG ACTCTGAGCC GACTGGCTGT GATGGAAAAA 
ATACTGCAGA TGTTCAAGTT GATCGTGGAG CAGCCAGGGA GCGCCTCCTT GGGGCTGCTT 
CCCTCCATTT TAGATTTTAG CACTCAGCAG ATTCTGCCCT TGTTGCAGCA GCAAAATGCG 
GCAACCGATA ACAGCGAGAT CACTAGCCTG TTGTACGCTT TGTTTGATAG TGTCCTCACC 
TGCAAGTGGC AGTTCTTCTA CAAAAATCAG CTCTCCAATG GTCACAGCGG TGGTGGCTCC 
GGCAACTTCA ACTGCAGCGA CAACCTGCAT CCAGCACACT TCATGGCCAT CCTGAATGCG 
TATGGCCAGA TGCTGGTCAG TGGGACTGAT CCGAGCAATG TGCGCAGCGT TCTCTTCTCA 
ATGCAAAATG TGAATGAGAG GAGTCGGTTG TACCAACGCG CCTTGTTCAA GGAGCAACTG 
CTGGCTTCAT TTCAGCGGGC CTTGCTCAAT CTGCTGTTGA GTGGAGAGGG CTCCCTCCAC 
TTCGACATGA TTGCCCAGGC GTTGTATGCT ATGGGCCAGG TGGACCGACG CCAGTTGCTC 
GAGAGCTTCG TTCACGCCTC TTTGCCAGTG GCTCAGACGT CGCTGGAGGA GATCTGCCAG 
ACAAATGTAC TACCTTTTAT TGTTCAGGAC ATTCCAACGT TTACGCAGAA ACTCACGCAG 
CTCATGCAGG ACGCCCACTG CGTGCACCTC AATGAGACGG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284739.1
CDS	184..3348
Translation	MHAASLTTVEGLLQEFYQPSTSNARKREIETNLLAFKSQPEAWQLCLRVATSSDTTENQFLWFFSTSTLEHTITRRWTQLTSTDKTLLRETLWNSYAQLVATPNVAKRHRDTLAQLIALLGKREFPEQDPNYMQHCMELTKTRFQLGINLLKITSEEVVSNRGDLTTEWKQYFYSCISMCVPDVLDLVTKYLLIAVCHINGKDIQTTIPNTLMDFSLTSALPNDNQLSSSILDLLGCVQHLVSWIRTELISEYFLMSILDLSQWRPAHEPISLAALSVLNELLYLQKPLPFPGPLMGGVTSLLEQHNNNRRQSEMYSDKFRELLRLYTTKYAAKLMQEPDLLETFLNLLYSCTTELHGALDFTEKLEIWTPIIKGIAQHPAKITRYNNVLTQLVDEIMRRTQFEANKPELEVLDNELMEDDTSATEWQQFLDQCFESLALLANTRGAHIVFAQVYNHWSRPQMYLLSLEHALDHGSARSYEAARKLKGANVGEILRDFATVCQAVVRLAPLMDSSTAAPGVADEMECQLQMLSDSLLQTLQLLASNRLYSGGSEMDKATFQTDLDNLYAQVLLAMRSIFPLTKAMSGEELLHRLFAILGSIFACNGSLTAASPIVQQAASELLLFISTVIRPKCLLDIPQIQSLLQAGSRLGAQLPRQVCINVHISLVSYMVLPWKSVPEQQQEYPRRLWMLREYVQGLAQNFLDLDLGQANESKVAATTLSLLGTFTALIEYFKATGGNAKDMLASTFKPILTKALMIFNNFGQSSIAMAITVAEFSLSMLRTLPTHLGAQFIKEMITLFISVSSREQLTLSRLAVMEKILQMFKLIVEQPGSASLGLLPSILDFSTQQILPLLQQQNAATDNSEITSLLYALFDSVLTCKWQFFYKNQLSNGHSGGGSGNFNCSDNLHPAHFMAILNAYGQMLVSGTDPSNVRSVLFSMQNVNERSRLYQRALFKEQLLASFQRALLNLLLSGEGSLHFDMIAQALYAMGQVDRRQLLESFVHASLPVAQTSLEEICQTNVLPFIVQDIPTFTQKLTQLMQDAHCVHLNETA

Target ORF information:

RefSeq Version	NM_001297810.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ellipsoid body open (ebo), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297810.1

ORF Insert Sequence:

ATGCACGCCG CATCTTTGAC GACGGTGGAG GGACTGCTGC AGGAGTTTTA TCAGCCCAGC 
ACCTCCAATG CCCGGAAACG AGAGATCGAG ACAAACCTCT TGGCCTTCAA GTCCCAGCCA 
GAGGCGTGGC AACTTTGCCT CCGTGTGGCC ACCTCTTCCG ACACCACGGA GAATCAGTTC 
CTCTGGTTCT TCAGCACCTC AACCTTGGAG CACACAATCA CGCGGAGATG GACACAGTTG 
ACGTCCACGG ACAAGACTTT GCTGCGGGAG ACCCTTTGGA ACTCGTATGC CCAACTGGTA 
GCCACTCCAA ATGTGGCCAA ACGGCACAGG GACACACTGG CCCAGTTGAT AGCCCTACTC 
GGAAAGAGGG AGTTCCCTGA ACAGGACCCC AATTACATGC AACACTGCAT GGAACTGACA 
AAAACACGGT TTCAACTTGG CATCAACCTG CTTAAGATCA CCTCCGAGGA GGTGGTAAGC 
AACAGGGGCG ACCTGACCAC CGAATGGAAG CAGTACTTCT ACTCCTGCAT CTCCATGTGT 
GTACCCGATG TTCTGGACCT GGTAACCAAG TATCTGCTAA TCGCTGTATG CCACATCAAT 
GGCAAAGACA TCCAAACAAC CATACCAAAC ACCCTCATGG ACTTTAGTCT AACCTCAGCG 
CTGCCAAATG ACAATCAATT AAGTTCCTCA ATTTTGGACC TGCTAGGATG TGTGCAGCAC 
TTGGTCTCCT GGATACGCAC TGAACTGATC TCGGAGTACT TCCTTATGAG CATACTTGAC 
CTTTCCCAGT GGCGACCAGC CCATGAGCCC ATCTCCTTGG CGGCTCTCTC TGTGTTGAAT 
GAGCTCCTCT ACCTCCAGAA GCCACTGCCC TTCCCAGGAC CCTTGATGGG CGGCGTTACC 
AGCCTCCTGG AGCAGCACAA TAACAATCGG CGGCAGAGCG AGATGTACAG CGACAAGTTT 
CGGGAGCTGC TGCGCCTCTA CACCACCAAG TATGCCGCCA AGCTGATGCA GGAGCCAGAC 
CTGCTGGAGA CCTTTCTCAA TCTGCTATAC AGCTGCACCA CTGAATTACA CGGCGCCTTG 
GACTTCACCG AAAAGCTTGA GATCTGGACA CCCATCATCA AAGGCATTGC CCAGCACCCC 
GCCAAAATCA CGCGTTATAA CAATGTGCTT ACCCAGCTAG TAGATGAGAT TATGAGGCGC 
ACTCAGTTTG AGGCCAACAA ACCCGAGCTA GAAGTGCTGG ACAACGAGCT CATGGAGGAC 
GATACCTCCG CCACTGAGTG GCAGCAGTTT CTAGACCAAT GCTTCGAGTC CCTGGCCCTA 
CTGGCCAACA CCAGAGGTGC ACACATCGTT TTCGCACAGG TCTATAACCA CTGGTCACGG 
CCACAGATGT ATTTACTGTC CTTGGAGCAC GCATTGGACC ACGGAAGCGC TCGAAGCTAC 
GAAGCGGCAC GTAAACTGAA GGGCGCCAAT GTGGGCGAGA TTTTGCGCGA CTTTGCCACC 
GTCTGCCAGG CGGTCGTGCG TTTGGCCCCA TTGATGGACT CATCAACAGC AGCACCCGGG 
GTTGCTGATG AGATGGAGTG CCAGCTGCAG ATGCTGTCCG ACAGTTTGCT GCAGACTCTA 
CAGCTCCTCG CAAGCAATCG TTTGTATAGC GGCGGCAGCG AAATGGACAA AGCTACTTTC 
CAGACTGATT TGGACAACCT ATACGCTCAA GTTCTACTGG CCATGAGGAG CATCTTTCCG 
CTAACGAAAG CCATGAGCGG CGAGGAACTG CTGCACCGCC TGTTTGCTAT CCTGGGCAGC 
ATTTTCGCAT GCAATGGATC CTTAACGGCT GCCTCCCCCA TTGTGCAGCA GGCGGCAAGC 
GAGCTGCTGC TTTTCATATC GACTGTGATA CGGCCCAAGT GCCTGCTGGA TATACCACAA 
ATCCAAAGCC TGCTGCAGGC AGGCTCTCGC TTGGGCGCCC AATTGCCGCG TCAGGTGTGC 
ATCAATGTAC ACATCAGTCT GGTCAGCTAC ATGGTGCTGC CGTGGAAAAG CGTTCCGGAG 
CAGCAGCAGG AGTATCCGAG GCGTCTCTGG ATGCTGCGTG AATATGTCCA GGGGCTGGCA 
CAGAACTTTC TGGATCTGGA CTTAGGCCAG GCCAACGAGA GCAAGGTGGC GGCCACCACG 
CTCAGTTTGC TGGGTACTTT CACTGCTCTC ATTGAGTATT TTAAAGCTAC TGGAGGCAAT 
GCCAAGGATA TGCTGGCAAG CACTTTCAAG CCTATTTTAA CAAAGGCCCT AATGATTTTT 
AACAACTTTG GGCAAAGCAG CATCGCCATG GCCATCACGG TGGCCGAGTT TAGCCTGAGC 
ATGCTCCGAA CATTGCCCAC CCATTTGGGG GCCCAGTTCA TTAAGGAAAT GATCACCTTG 
TTCATAAGTG TCAGCAGTAG GGAGCAACTG ACTCTGAGCC GACTGGCTGT GATGGAAAAA 
ATACTGCAGA TGTTCAAGTT GATCGTGGAG CAGCCAGGGA GCGCCTCCTT GGGGCTGCTT 
CCCTCCATTT TAGATTTTAG CACTCAGCAG ATTCTGCCCT TGTTGCAGCA GCAAAATGCG 
GCAACCGATA ACAGCGAGAT CACTAGCCTG TTGTACGCTT TGTTTGATAG TGTCCTCACC 
TGCAAGTGGC AGTTCTTCTA CAAAAATCAG CTCTCCAATG GTCACAGCGG TGGTGGCTCC 
GGCAACTTCA ACTGCAGCGA CAACCTGCAT CCAGCACACT TCATGGCCAT CCTGAATGCG 
TATGGCCAGA TGCTGGTCAG TGGGACTGAT CCGAGCAATG TGCGCAGCGT TCTCTTCTCA 
ATGCAAAATG TGAATGAGAG GAGTCGGTTG TACCAACGCG CCTTGTTCAA GGAGCAACTG 
CTGGCTTCAT TTCAGCGGGC CTTGCTCAAT CTGCTGTTGA GTGGAGAGGG CTCCCTCCAC 
TTCGACATGA TTGCCCAGGC GTTGTATGCT ATGGGCCAGG TGGACCGACG CCAGTTGCTC 
GAGAGCTTCG TTCACGCCTC TTTGCCAGTG GCTCAGACGT CGCTGGAGGA GATCTGCCAG 
ACAAATGTAC TACCTTTTAT TGTTCAGGAC ATTCCAACGT TTACGCAGAA ACTCACGCAG 
CTCATGCAGG ACGCCCACTG CGTGCACCTC AATGAGACGG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ebo ( NM_001297809.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284738.1 Drosophila melanogaster ellipsoid body open (ebo), transcript variant B, mRNA.

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CloneID	OFa22262
Clone ID Related Accession (Same CDS sequence)	NM_001297809.1
Accession Version	NM_001297809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ellipsoid body open, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGCCG CATCTTTGAC GACGGTGGAG GGACTGCTGC AGGAGTTTTA TCAGCCCAGC 
ACCTCCAATG CCCGGAAACG AGAGATCGAG ACAAACCTCT TGGCCTTCAA GTCCCAGCCA 
GAGGCGTGGC AACTTTGCCT CCGTGTGGCC ACCTCTTCCG ACACCACGGA GAATCAGTTC 
CTCTGGTTCT TCAGCACCTC AACCTTGGAG CACACAATCA CGCGGAGATG GACACAGTTG 
ACGTCCACGG ACAAGACTTT GCTGCGGGAG ACCCTTTGGA ACTCGTATGC CCAACTGGTA 
GCCACTCCAA ATGTGGCCAA ACGGCACAGG GACACACTGG CCCAGTTGAT AGCCCTACTC 
GGAAAGAGGG AGTTCCCTGA ACAGGACCCC AATTACATGC AACACTGCAT GGAACTGACA 
AAAACACGGT TTCAACTTGG CATCAACCTG CTTAAGATCA CCTCCGAGGA GGTGGTAAGC 
AACAGGGGCG ACCTGACCAC CGAATGGAAG CAGTACTTCT ACTCCTGCAT CTCCATGTGT 
GTACCCGATG TTCTGGACCT GGTAACCAAG TATCTGCTAA TCGCTGTATG CCACATCAAT 
GGCAAAGACA TCCAAACAAC CATACCAAAC ACCCTCATGG ACTTTAGTCT AACCTCAGCG 
CTGCCAAATG ACAATCAATT AAGTTCCTCA ATTTTGGACC TGCTAGGATG TGTGCAGCAC 
TTGGTCTCCT GGATACGCAC TGAACTGATC TCGGAGTACT TCCTTATGAG CATACTTGAC 
CTTTCCCAGT GGCGACCAGC CCATGAGCCC ATCTCCTTGG CGGCTCTCTC TGTGTTGAAT 
GAGCTCCTCT ACCTCCAGAA GCCACTGCCC TTCCCAGGAC CCTTGATGGG CGGCGTTACC 
AGCCTCCTGG AGCAGCACAA TAACAATCGG CGGCAGAGCG AGATGTACAG CGACAAGTTT 
CGGGAGCTGC TGCGCCTCTA CACCACCAAG TATGCCGCCA AGCTGATGCA GGAGCCAGAC 
CTGCTGGAGA CCTTTCTCAA TCTGCTATAC AGCTGCACCA CTGAATTACA CGGCGCCTTG 
GACTTCACCG AAAAGCTTGA GATCTGGACA CCCATCATCA AAGGCATTGC CCAGCACCCC 
GCCAAAATCA CGCGTTATAA CAATGTGCTT ACCCAGCTAG TAGATGAGAT TATGAGGCGC 
ACTCAGTTTG AGGCCAACAA ACCCGAGCTA GAAGTGCTGG ACAACGAGCT CATGGAGGAC 
GATACATTCA TCCAATTTCA GACCTCCGCC ACTGAGTGGC AGCAGTTTCT AGACCAATGC 
TTCGAGTCCC TGGCCCTACT GGCCAACACC AGAGGTGCAC ACATCGTTTT CGCACAGGTC 
TATAACCACT GGTCACGGCC ACAGATGTAT TTACTGTCCT TGGAGCACGC ATTGGACCAC 
GGAAGCGCTC GAAGCTACGA AGCGGCACGT AAACTGAAGG GCGCCAATGT GGGCGAGATT 
TTGCGCGACT TTGCCACCGT CTGCCAGGCG GTCGTGCGTT TGGCCCCATT GATGGACTCA 
TCAACAGCAG CACCCGGGGT TGCTGATGAG ATGGAGTGCC AGCTGCAGAT GCTGTCCGAC 
AGTTTGCTGC AGACTCTACA GCTCCTCGCA AGCAATCGTT TGTATAGCGG CGGCAGCGAA 
ATGGACAAAG CTACTTTCCA GACTGATTTG GACAACCTAT ACGCTCAAGT TCTACTGGCC 
ATGAGGAGCA TCTTTCCGCT AACGAAAGCC ATGAGCGGCG AGGAACTGCT GCACCGCCTG 
TTTGCTATCC TGGGCAGCAT TTTCGCATGC AATGGATCCT TAACGGCTGC CTCCCCCATT 
GTGCAGCAGG CGGCAAGCGA GCTGCTGCTT TTCATATCGA CTGTGATACG GCCCAAGTGC 
CTGCTGGATA TACCACAAAT CCAAAGCCTG CTGCAGGCAG GCTCTCGCTT GGGCGCCCAA 
TTGCCGCGTC AGGTGTGCAT CAATGTACAC ATCAGTCTGG TCAGCTACAT GGTGCTGCCG 
TGGAAAAGCG TTCCGGAGCA GCAGCAGGAG TATCCGAGGC GTCTCTGGAT GCTGCGTGAA 
TATGTCCAGG GGCTGGCACA GAACTTTCTG GATCTGGACT TAGGCCAGGC CAACGAGAGC 
AAGGTGGCGG CCACCACGCT CAGTTTGCTG GGTACTTTCA CTGCTCTCAT TGAGTATTTT 
AAAGCTACTG GAGGCAATGC CAAGGATATG CTGGCAAGCA CTTTCAAGCC TATTTTAACA 
AAGGCCCTAA TGATTTTTAA CAACTTTGGG CAAAGCAGCA TCGCCATGGC CATCACGGTG 
GCCGAGTTTA GCCTGAGCAT GCTCCGAACA TTGCCCACCC ATTTGGGGGC CCAGTTCATT 
AAGGAAATGA TCACCTTGTT CATAAGTGTC AGCAGTAGGG AGCAACTGAC TCTGAGCCGA 
CTGGCTGTGA TGGAAAAAAT ACTGCAGATG TTCAAGTTGA TCGTGGAGCA GCCAGGGAGC 
GCCTCCTTGG GGCTGCTTCC CTCCATTTTA GATTTTAGCA CTCAGCAGAT TCTGCCCTTG 
TTGCAGCAGC AAAATGCGGC AACCGATAAC AGCGAGATCA CTAGCCTGTT GTACGCTTTG 
TTTGATAGTG TCCTCACCTG CAAGTGGCAG TTCTTCTACA AAAATCAGCT CTCCAATGGT 
CACAGCGGTG GTGGCTCCGG CAACTTCAAC TGCAGCGACA ACCTGCATCC AGCACACTTC 
ATGGCCATCC TGAATGCGTA TGGCCAGATG CTGGTCAGTG GGACTGATCC GAGCAATGTG 
CGCAGCGTTC TCTTCTCAAT GCAAAATGTG AATGAGAGGA GTCGGTTGTA CCAACGCGCC 
TTGTTCAAGG AGCAACTGCT GGCTTCATTT CAGCGGGCCT TGCTCAATCT GCTGTTGAGT 
GGAGAGGGCT CCCTCCACTT CGACATGATT GCCCAGGCGT TGTATGCTAT GGGCCAGGTG 
GACCGACGCC AGTTGCTCGA GAGCTTCGTT CACGCCTCTT TGCCAGTGGC TCAGACGTCG 
CTGGAGGAGA TCTGCCAGAC AAATGACATT CCAACGTTTA CGCAGAAACT CACGCAGCTC 
ATGCAGGACG CCCACTGCGT GCACCTCAAT GAGACGGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284738.1
CDS	184..3345
Translation	MHAASLTTVEGLLQEFYQPSTSNARKREIETNLLAFKSQPEAWQLCLRVATSSDTTENQFLWFFSTSTLEHTITRRWTQLTSTDKTLLRETLWNSYAQLVATPNVAKRHRDTLAQLIALLGKREFPEQDPNYMQHCMELTKTRFQLGINLLKITSEEVVSNRGDLTTEWKQYFYSCISMCVPDVLDLVTKYLLIAVCHINGKDIQTTIPNTLMDFSLTSALPNDNQLSSSILDLLGCVQHLVSWIRTELISEYFLMSILDLSQWRPAHEPISLAALSVLNELLYLQKPLPFPGPLMGGVTSLLEQHNNNRRQSEMYSDKFRELLRLYTTKYAAKLMQEPDLLETFLNLLYSCTTELHGALDFTEKLEIWTPIIKGIAQHPAKITRYNNVLTQLVDEIMRRTQFEANKPELEVLDNELMEDDTFIQFQTSATEWQQFLDQCFESLALLANTRGAHIVFAQVYNHWSRPQMYLLSLEHALDHGSARSYEAARKLKGANVGEILRDFATVCQAVVRLAPLMDSSTAAPGVADEMECQLQMLSDSLLQTLQLLASNRLYSGGSEMDKATFQTDLDNLYAQVLLAMRSIFPLTKAMSGEELLHRLFAILGSIFACNGSLTAASPIVQQAASELLLFISTVIRPKCLLDIPQIQSLLQAGSRLGAQLPRQVCINVHISLVSYMVLPWKSVPEQQQEYPRRLWMLREYVQGLAQNFLDLDLGQANESKVAATTLSLLGTFTALIEYFKATGGNAKDMLASTFKPILTKALMIFNNFGQSSIAMAITVAEFSLSMLRTLPTHLGAQFIKEMITLFISVSSREQLTLSRLAVMEKILQMFKLIVEQPGSASLGLLPSILDFSTQQILPLLQQQNAATDNSEITSLLYALFDSVLTCKWQFFYKNQLSNGHSGGGSGNFNCSDNLHPAHFMAILNAYGQMLVSGTDPSNVRSVLFSMQNVNERSRLYQRALFKEQLLASFQRALLNLLLSGEGSLHFDMIAQALYAMGQVDRRQLLESFVHASLPVAQTSLEEICQTNDIPTFTQKLTQLMQDAHCVHLNETA

Target ORF information:

RefSeq Version	NM_001297809.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ellipsoid body open (ebo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297809.1

ORF Insert Sequence:

ATGCACGCCG CATCTTTGAC GACGGTGGAG GGACTGCTGC AGGAGTTTTA TCAGCCCAGC 
ACCTCCAATG CCCGGAAACG AGAGATCGAG ACAAACCTCT TGGCCTTCAA GTCCCAGCCA 
GAGGCGTGGC AACTTTGCCT CCGTGTGGCC ACCTCTTCCG ACACCACGGA GAATCAGTTC 
CTCTGGTTCT TCAGCACCTC AACCTTGGAG CACACAATCA CGCGGAGATG GACACAGTTG 
ACGTCCACGG ACAAGACTTT GCTGCGGGAG ACCCTTTGGA ACTCGTATGC CCAACTGGTA 
GCCACTCCAA ATGTGGCCAA ACGGCACAGG GACACACTGG CCCAGTTGAT AGCCCTACTC 
GGAAAGAGGG AGTTCCCTGA ACAGGACCCC AATTACATGC AACACTGCAT GGAACTGACA 
AAAACACGGT TTCAACTTGG CATCAACCTG CTTAAGATCA CCTCCGAGGA GGTGGTAAGC 
AACAGGGGCG ACCTGACCAC CGAATGGAAG CAGTACTTCT ACTCCTGCAT CTCCATGTGT 
GTACCCGATG TTCTGGACCT GGTAACCAAG TATCTGCTAA TCGCTGTATG CCACATCAAT 
GGCAAAGACA TCCAAACAAC CATACCAAAC ACCCTCATGG ACTTTAGTCT AACCTCAGCG 
CTGCCAAATG ACAATCAATT AAGTTCCTCA ATTTTGGACC TGCTAGGATG TGTGCAGCAC 
TTGGTCTCCT GGATACGCAC TGAACTGATC TCGGAGTACT TCCTTATGAG CATACTTGAC 
CTTTCCCAGT GGCGACCAGC CCATGAGCCC ATCTCCTTGG CGGCTCTCTC TGTGTTGAAT 
GAGCTCCTCT ACCTCCAGAA GCCACTGCCC TTCCCAGGAC CCTTGATGGG CGGCGTTACC 
AGCCTCCTGG AGCAGCACAA TAACAATCGG CGGCAGAGCG AGATGTACAG CGACAAGTTT 
CGGGAGCTGC TGCGCCTCTA CACCACCAAG TATGCCGCCA AGCTGATGCA GGAGCCAGAC 
CTGCTGGAGA CCTTTCTCAA TCTGCTATAC AGCTGCACCA CTGAATTACA CGGCGCCTTG 
GACTTCACCG AAAAGCTTGA GATCTGGACA CCCATCATCA AAGGCATTGC CCAGCACCCC 
GCCAAAATCA CGCGTTATAA CAATGTGCTT ACCCAGCTAG TAGATGAGAT TATGAGGCGC 
ACTCAGTTTG AGGCCAACAA ACCCGAGCTA GAAGTGCTGG ACAACGAGCT CATGGAGGAC 
GATACATTCA TCCAATTTCA GACCTCCGCC ACTGAGTGGC AGCAGTTTCT AGACCAATGC 
TTCGAGTCCC TGGCCCTACT GGCCAACACC AGAGGTGCAC ACATCGTTTT CGCACAGGTC 
TATAACCACT GGTCACGGCC ACAGATGTAT TTACTGTCCT TGGAGCACGC ATTGGACCAC 
GGAAGCGCTC GAAGCTACGA AGCGGCACGT AAACTGAAGG GCGCCAATGT GGGCGAGATT 
TTGCGCGACT TTGCCACCGT CTGCCAGGCG GTCGTGCGTT TGGCCCCATT GATGGACTCA 
TCAACAGCAG CACCCGGGGT TGCTGATGAG ATGGAGTGCC AGCTGCAGAT GCTGTCCGAC 
AGTTTGCTGC AGACTCTACA GCTCCTCGCA AGCAATCGTT TGTATAGCGG CGGCAGCGAA 
ATGGACAAAG CTACTTTCCA GACTGATTTG GACAACCTAT ACGCTCAAGT TCTACTGGCC 
ATGAGGAGCA TCTTTCCGCT AACGAAAGCC ATGAGCGGCG AGGAACTGCT GCACCGCCTG 
TTTGCTATCC TGGGCAGCAT TTTCGCATGC AATGGATCCT TAACGGCTGC CTCCCCCATT 
GTGCAGCAGG CGGCAAGCGA GCTGCTGCTT TTCATATCGA CTGTGATACG GCCCAAGTGC 
CTGCTGGATA TACCACAAAT CCAAAGCCTG CTGCAGGCAG GCTCTCGCTT GGGCGCCCAA 
TTGCCGCGTC AGGTGTGCAT CAATGTACAC ATCAGTCTGG TCAGCTACAT GGTGCTGCCG 
TGGAAAAGCG TTCCGGAGCA GCAGCAGGAG TATCCGAGGC GTCTCTGGAT GCTGCGTGAA 
TATGTCCAGG GGCTGGCACA GAACTTTCTG GATCTGGACT TAGGCCAGGC CAACGAGAGC 
AAGGTGGCGG CCACCACGCT CAGTTTGCTG GGTACTTTCA CTGCTCTCAT TGAGTATTTT 
AAAGCTACTG GAGGCAATGC CAAGGATATG CTGGCAAGCA CTTTCAAGCC TATTTTAACA 
AAGGCCCTAA TGATTTTTAA CAACTTTGGG CAAAGCAGCA TCGCCATGGC CATCACGGTG 
GCCGAGTTTA GCCTGAGCAT GCTCCGAACA TTGCCCACCC ATTTGGGGGC CCAGTTCATT 
AAGGAAATGA TCACCTTGTT CATAAGTGTC AGCAGTAGGG AGCAACTGAC TCTGAGCCGA 
CTGGCTGTGA TGGAAAAAAT ACTGCAGATG TTCAAGTTGA TCGTGGAGCA GCCAGGGAGC 
GCCTCCTTGG GGCTGCTTCC CTCCATTTTA GATTTTAGCA CTCAGCAGAT TCTGCCCTTG 
TTGCAGCAGC AAAATGCGGC AACCGATAAC AGCGAGATCA CTAGCCTGTT GTACGCTTTG 
TTTGATAGTG TCCTCACCTG CAAGTGGCAG TTCTTCTACA AAAATCAGCT CTCCAATGGT 
CACAGCGGTG GTGGCTCCGG CAACTTCAAC TGCAGCGACA ACCTGCATCC AGCACACTTC 
ATGGCCATCC TGAATGCGTA TGGCCAGATG CTGGTCAGTG GGACTGATCC GAGCAATGTG 
CGCAGCGTTC TCTTCTCAAT GCAAAATGTG AATGAGAGGA GTCGGTTGTA CCAACGCGCC 
TTGTTCAAGG AGCAACTGCT GGCTTCATTT CAGCGGGCCT TGCTCAATCT GCTGTTGAGT 
GGAGAGGGCT CCCTCCACTT CGACATGATT GCCCAGGCGT TGTATGCTAT GGGCCAGGTG 
GACCGACGCC AGTTGCTCGA GAGCTTCGTT CACGCCTCTT TGCCAGTGGC TCAGACGTCG 
CTGGAGGAGA TCTGCCAGAC AAATGACATT CCAACGTTTA CGCAGAAACT CACGCAGCTC 
ATGCAGGACG CCCACTGCGT GCACCTCAAT GAGACGGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ebo cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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