l(2)01289 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following l(2)01289 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the l(2)01289 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa11421 NM_001259223.2
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant L, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa12721 NM_001043009.3
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa12724 NM_143769.6
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa11419 NM_001169595.2
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa11420 NM_001169594.2
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa12722 NM_001043010.4
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant K, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa12723 NM_165485.3
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OFa11422 NM_001169593.2
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OFa11423 NM_001259224.2
Latest version!
Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa11421
    Clone ID Related Accession (Same CDS sequence) NM_001259223.2
    Accession Version NM_001259223.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2616bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform L
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259223.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAACTTGGG GAAGAGGAAT TCAAATAGAA ATGCATTTAA CCAAGATGAC
    GAATGTTTCT ATGTTGGATT GGGTCATGAC GGCCATTCAG CTAAGCGCGG CAACAATTTC
    GTGCCCAACG ATTACAAACC ATTCCAATGC TGTCCAACCA AATTGGAGAA GTCAACGAAA
    GTTCCTAAGA TGACCGCCCA GCGGATCGGG CATAGCGAAG GCGATCAGGG CAAGCGTCCC
    AGCGGCGGCA ACTTCCAGTT TGCCAACCAG GCGTCCAGCA AATCGTCCAC CAAGCCAGCT
    GCCATCAAGA AGCAGGCCAA GCCCTCCAAG GACACCGATG ACGACGATGA GGACGACGAG
    GACAAGCCCC TGGTCAAGGT TTCCTATGCC AACAAGCGCT CGGGAGGAAG CAACAAGCCG
    CAGGCTGGCA AGAAGCCGGT AGGCAAGGGC CAGGACAACG ACGACCAGTC CCAGGAGGTG
    GAGAAGGTAT CCAAACAGAA GTCGGCGAAG AAGTCCGGCA AACTGAATGT CAAATCCGGA
    TATCTCTCTG TGGGAGTAAG GCAACAGTTT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246152.1
    CDS421..3036
    Translation

    Target ORF information:

    RefSeq Version NM_001259223.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant L, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259223.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAACTTGGG GAAGAGGAAT TCAAATAGAA ATGCATTTAA CCAAGATGAC
    GAATGTTTCT ATGTTGGATT GGGTCATGAC GGCCATTCAG CTAAGCGCGG CAACAATTTC
    GTGCCCAACG ATTACAAACC ATTCCAATGC TGTCCAACCA AATTGGAGAA GTCAACGAAA
    GTTCCTAAGA TGACCGCCCA GCGGATCGGG CATAGCGAAG GCGATCAGGG CAAGCGTCCC
    AGCGGCGGCA ACTTCCAGTT TGCCAACCAG GCGTCCAGCA AATCGTCCAC CAAGCCAGCT
    GCCATCAAGA AGCAGGCCAA GCCCTCCAAG GACACCGATG ACGACGATGA GGACGACGAG
    GACAAGCCCC TGGTCAAGGT TTCCTATGCC AACAAGCGCT CGGGAGGAAG CAACAAGCCG
    CAGGCTGGCA AGAAGCCGGT AGGCAAGGGC CAGGACAACG ACGACCAGTC CCAGGAGGTG
    GAGAAGGTAT CCAAACAGAA GTCGGCGAAG AAGTCCGGCA AACTGAATGT CAAATCCGGA
    TATCTCTCTG TGGGAGTAAG GCAACAGTTT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12721
    Clone ID Related Accession (Same CDS sequence) NM_001043009.3
    Accession Version NM_001043009.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2523bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001043009.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    AAAGAAGATG ATCCCGACTC GCCACGAGTG CTGCGGCACA TCGAACTAAT CGACGACGAG
    GCTGCGGAAT ATGGCATTTA CATAGTGAAG ATGCACGACA AACTGATGGC CAAGAAGTAC
    GGCTTCCGGA ATCCCCCAGG ACTGACGTAT TTCCGAAAGG GCAAGTATAT CAACTACGAC
    GGCGATATCG ATGACGAGGA GGAGGTACTG GACTGGCTAA CGAGCCCGGC CAACATGGAG
    ATGACCGATC ACATCGAGCA GGTTAACCGC AAGATGTTCG AGAAGATCCG CAAGAACTCC
    GACTACGTAG CGGTGATATT CTATAGCGAT GAGTGCAAGC AGTGTCCTCG CGTCCTGGCC
    GAGGTGGAAC ACATAGACGA CGAAGCGGAC AAGGCTGGTA TCGACTTCGT CAAAATCGAC
    GATAAGCAGA TGGCCAAGGA GTACGGAGTG TTCGCCCTGC CTGCCATTGT CTTCTTCAAG
    CCCACATCCA AGGAGCCAGT TATATACGCC GGTGATCTTT ACGAAGAAGA ACAGATCCTA
    ACTTGGCTGA TCACGCAAAA GGATCCAAGT GGAGATGTTA TCGAAGATCT CGAAGGCGAA
    AGACTTGTTC ATCTGATTGA AGAGTCTGGC TCCATCGCAG TCTACTTTTA TGCGGATGGC
    TGCGAGCAGT GCACCAAGGT TTTGGAGGAG TTGGAAAACA TCGATGATGA TTGCGACAAG
    CATGGCATAA CATTCGTTAA GACCAGGGAC TTCTCTGTGG CCGACGGCTA TGGCGTGCAC
    GAGTATCCGG CTCTAGTTTA CTTCGAGGGA GGAATCCCCA ACGTATTCGA GGGCGAATTG
    AGCGAGGAGG AAGAGGTTCT TCAGTGGCTT ATCACCCAGA AGACCGAAGA TCGTATTGAG
    CTGATCACTC GCCAGATGCT GGAGACAATG GTGGAGGAGA CGCAGTACCT AGCCGTGTAT
    TTCTACAAAA TCAACTGCAA CATATGCGAC CAGATACTAG AGGGCTTGGA ACTGATCGAT
    GACGAATGCG ACGTGTTCGG AATTCATATG GTCAAGATCC AGGATCCGCA GCTGGCAAAA
    CGTTACTCGA TCAAGACTTT CCCGGCGTTG GTTTATTTCA GAAATGGAAA CCCATTACTG
    TTTGAGGGGG ATCTTCAGAA CGAGCAATCA GTATTGGAAT GGCTCATCGA TGATGACAAC
    CGCGAGCTGG CTGATGAAAT CGAGGAGGTC AACGAGCGTA TGCTGGATCG CCTAATGGCG
    GAGTCCACTC TATTGGTTGT TTTCTTTTAC GACGATGATT GTGCTGAGTG CGAGGAGATT
    TTGGAAGAGC TGGAGGAGAT CGATGGTGAG GCAGACATGT TCGGCATTGA TTTCGTAAAG
    ATTGCTAGTA TCCAGGCAGC CAAGAAATAC GAGATAGTGA ATATACCTTC CCTCGTTTAT
    TTCCGAAAAC AAGTGCCCGT CCTCTACGAC GGCGATCTAC ACCAACACGA CAAGGTGATT
    ACCTGGCTAA CATCACAGGA CGTATTCGAA ATCAAAAACG AAATAGAAGA AGTAAACCGA
    AAAATGCTCG ACAAACTACT CGAGGAAAAT GAGTTCTTAG CCGTTTTTTT CTACGAACAC
    AATCAGCCGG ACAGTACTGC AGCGCTGGAA AAACTCGAAA ACATCGACAG CGAGACGGAT
    AACCTGGACA TCACCTTCGT CAAGATGGCC GACTCTCGTT ATGCCAAGAA ATGGGGCGTC
    ACCAAGCTGC CGGCAATGGT CTACTTCCGC CGTCGGTTTC CCAGCATATA TAGGGGTGAT
    CTGTTATCCG AGGACGAAGT GCTGGAGTGG CTACGCAAGA ACCGCTTCCG CCAGCCCGAG
    CTGAACATCT TTATGTACGC TCTGATTGCT CTGGCGGTGG CCTTTGTGGT CTACACCGCC
    TTCCTGCTGC AGTGCTTCAA GCCGGCGCCA CCGCCTCCTG TCCAGCACCC CAAGCAGTCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036474.2
    CDS421..2943
    Translation

    Target ORF information:

    RefSeq Version NM_001043009.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001043009.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    AAAGAAGATG ATCCCGACTC GCCACGAGTG CTGCGGCACA TCGAACTAAT CGACGACGAG
    GCTGCGGAAT ATGGCATTTA CATAGTGAAG ATGCACGACA AACTGATGGC CAAGAAGTAC
    GGCTTCCGGA ATCCCCCAGG ACTGACGTAT TTCCGAAAGG GCAAGTATAT CAACTACGAC
    GGCGATATCG ATGACGAGGA GGAGGTACTG GACTGGCTAA CGAGCCCGGC CAACATGGAG
    ATGACCGATC ACATCGAGCA GGTTAACCGC AAGATGTTCG AGAAGATCCG CAAGAACTCC
    GACTACGTAG CGGTGATATT CTATAGCGAT GAGTGCAAGC AGTGTCCTCG CGTCCTGGCC
    GAGGTGGAAC ACATAGACGA CGAAGCGGAC AAGGCTGGTA TCGACTTCGT CAAAATCGAC
    GATAAGCAGA TGGCCAAGGA GTACGGAGTG TTCGCCCTGC CTGCCATTGT CTTCTTCAAG
    CCCACATCCA AGGAGCCAGT TATATACGCC GGTGATCTTT ACGAAGAAGA ACAGATCCTA
    ACTTGGCTGA TCACGCAAAA GGATCCAAGT GGAGATGTTA TCGAAGATCT CGAAGGCGAA
    AGACTTGTTC ATCTGATTGA AGAGTCTGGC TCCATCGCAG TCTACTTTTA TGCGGATGGC
    TGCGAGCAGT GCACCAAGGT TTTGGAGGAG TTGGAAAACA TCGATGATGA TTGCGACAAG
    CATGGCATAA CATTCGTTAA GACCAGGGAC TTCTCTGTGG CCGACGGCTA TGGCGTGCAC
    GAGTATCCGG CTCTAGTTTA CTTCGAGGGA GGAATCCCCA ACGTATTCGA GGGCGAATTG
    AGCGAGGAGG AAGAGGTTCT TCAGTGGCTT ATCACCCAGA AGACCGAAGA TCGTATTGAG
    CTGATCACTC GCCAGATGCT GGAGACAATG GTGGAGGAGA CGCAGTACCT AGCCGTGTAT
    TTCTACAAAA TCAACTGCAA CATATGCGAC CAGATACTAG AGGGCTTGGA ACTGATCGAT
    GACGAATGCG ACGTGTTCGG AATTCATATG GTCAAGATCC AGGATCCGCA GCTGGCAAAA
    CGTTACTCGA TCAAGACTTT CCCGGCGTTG GTTTATTTCA GAAATGGAAA CCCATTACTG
    TTTGAGGGGG ATCTTCAGAA CGAGCAATCA GTATTGGAAT GGCTCATCGA TGATGACAAC
    CGCGAGCTGG CTGATGAAAT CGAGGAGGTC AACGAGCGTA TGCTGGATCG CCTAATGGCG
    GAGTCCACTC TATTGGTTGT TTTCTTTTAC GACGATGATT GTGCTGAGTG CGAGGAGATT
    TTGGAAGAGC TGGAGGAGAT CGATGGTGAG GCAGACATGT TCGGCATTGA TTTCGTAAAG
    ATTGCTAGTA TCCAGGCAGC CAAGAAATAC GAGATAGTGA ATATACCTTC CCTCGTTTAT
    TTCCGAAAAC AAGTGCCCGT CCTCTACGAC GGCGATCTAC ACCAACACGA CAAGGTGATT
    ACCTGGCTAA CATCACAGGA CGTATTCGAA ATCAAAAACG AAATAGAAGA AGTAAACCGA
    AAAATGCTCG ACAAACTACT CGAGGAAAAT GAGTTCTTAG CCGTTTTTTT CTACGAACAC
    AATCAGCCGG ACAGTACTGC AGCGCTGGAA AAACTCGAAA ACATCGACAG CGAGACGGAT
    AACCTGGACA TCACCTTCGT CAAGATGGCC GACTCTCGTT ATGCCAAGAA ATGGGGCGTC
    ACCAAGCTGC CGGCAATGGT CTACTTCCGC CGTCGGTTTC CCAGCATATA TAGGGGTGAT
    CTGTTATCCG AGGACGAAGT GCTGGAGTGG CTACGCAAGA ACCGCTTCCG CCAGCCCGAG
    CTGAACATCT TTATGTACGC TCTGATTGCT CTGGCGGTGG CCTTTGTGGT CTACACCGCC
    TTCCTGCTGC AGTGCTTCAA GCCGGCGCCA CCGCCTCCTG TCCAGCACCC CAAGCAGTCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12724
    Clone ID Related Accession (Same CDS sequence) NM_143769.6
    Accession Version NM_143769.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_143769.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAATGACGA ATGTTTCTAT GTTGGATTGG GTCATGACGG CCATTCAGCT
    AAGCGCGGCA ACAATTTCGT GCCCAACGAT TACAAACCAT TCCAATGCTG TCCAACCAAA
    TTGGAGAAGT CAACGAAAGT TCCTAAGATG ACCGCCCAGC GGATCGGGCA TAGCGAAGGC
    GATCAGGGCA AGCGTCCCAG CGGCGGCAAC TTCCAGTTTG CCAACCAGGC GTCCAGCAAA
    TCGTCCACCA AGCCAGCTGC CATCAAGAAG CAGGCCAAGC CCTCCAAGGA CACCGATGAC
    GACGATGAGG ACGACGAGGA CAAGCCCCTG GTCAAGGTTT CCTATGCCAA CAAGCGCTCG
    GGAGGAAGCA ACAAGCCGCA GGCTGGCAAG AAGCCGGTAG GCAAGGGCCA GGACAACGAC
    GACCAGTCCC AGGAGGTGGA GAAGGTATCC AAACAGAAGT CGGCGAAGAA GTCCGGCAAA
    CTGAATGTCA AATCCGGATA TCTCTCTGTG GGAGTAAGGC AACAGTTTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_652026.2
    CDS421..2994
    Translation

    Target ORF information:

    RefSeq Version NM_143769.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_143769.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAATGACGA ATGTTTCTAT GTTGGATTGG GTCATGACGG CCATTCAGCT
    AAGCGCGGCA ACAATTTCGT GCCCAACGAT TACAAACCAT TCCAATGCTG TCCAACCAAA
    TTGGAGAAGT CAACGAAAGT TCCTAAGATG ACCGCCCAGC GGATCGGGCA TAGCGAAGGC
    GATCAGGGCA AGCGTCCCAG CGGCGGCAAC TTCCAGTTTG CCAACCAGGC GTCCAGCAAA
    TCGTCCACCA AGCCAGCTGC CATCAAGAAG CAGGCCAAGC CCTCCAAGGA CACCGATGAC
    GACGATGAGG ACGACGAGGA CAAGCCCCTG GTCAAGGTTT CCTATGCCAA CAAGCGCTCG
    GGAGGAAGCA ACAAGCCGCA GGCTGGCAAG AAGCCGGTAG GCAAGGGCCA GGACAACGAC
    GACCAGTCCC AGGAGGTGGA GAAGGTATCC AAACAGAAGT CGGCGAAGAA GTCCGGCAAA
    CTGAATGTCA AATCCGGATA TCTCTCTGTG GGAGTAAGGC AACAGTTTAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa11419
    Clone ID Related Accession (Same CDS sequence) NM_001169595.2
    Accession Version NM_001169595.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169595.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAACATT GCAAGCAATC AGGACGTCTT CAACTGGATT
    CTCGAACAGA AGGCCGACCA AAGCATTCAA CTCATTAATC GTGACCAACT TTTCGAGTAT
    ATAGGCACCA AAGACTTTTT AGCGGTTGTT TTTTACAAAG AAGATGATCC CGACTCGCCA
    CGAGTGCTGC GGCACATCGA ACTAATCGAC GACGAGGCTG CGGAATATGG CATTTACATA
    GTGAAGATGC ACGACAAACT GATGGCCAAG AAGTACGGCT TCCGGAATCC CCCAGGACTG
    ACGTATTTCC GAAAGGGCAA GTATATCAAC TACGACGGCG ATATCGATGA CGAGGAGGAG
    GTACTGGACT GGCTAACGAG CCCGGCCAAC ATGGAGATGA CCGATCACAT CGAGCAGGTT
    AACCGCAAGA TGTTCGAGAA GATCCGCAAG AACTCCGACT ACGTAGCGGT GATATTCTAT
    AGCGATGAGT GCAAGCAGTG TCCTCGCGTC CTGGCCGAGG TGGAACACAT AGACGACGAA
    GCGGACAAGG CTGGTATCGA CTTCGTCAAA ATCGACGATA AGCAGATGGC CAAGGAGTAC
    GGAGTGTTCG CCCTGCCTGC CATTGTCTTC TTCAAGCCCA CATCCAAGGA GCCAGTTATA
    TACGCCGGTG ATCTTTACGA AGAAGAACAG ATCCTAACTT GGCTGATCAC GCAAAAGGAT
    CCAAGTGGAG ATGTTATCGA AGATCTCGAA GGCGAAAGAC TTGTTCATCT GATTGAAGAG
    TCTGGCTCCA TCGCAGTCTA CTTTTATGCG GATGGCTGCG AGCAGTGCAC CAAGGTTTTG
    GAGGAGTTGG AAAACATCGA TGATGATTGC GACAAGCATG GCATAACATT CGTTAAGACC
    AGGGACTTCT CTGTGGCCGA CGGCTATGGC GTGCACGAGT ATCCGGCTCT AGTTTACTTC
    GAGGGAGGAA TCCCCAACGT ATTCGAGGGC GAATTGAGCG AGGAGGAAGA GGTTCTTCAG
    TGGCTTATCA CCCAGAAGAC CGAAGATCGT ATTGAGCTGA TCACTCGCCA GATGCTGGAG
    ACAATGGTGG AGGAGACGCA GTACCTAGCC GTGTATTTCT ACAAAATCAA CTGCAACATA
    TGCGACCAGA TACTAGAGGG CTTGGAACTG ATCGATGACG AATGCGACGT GTTCGGAATT
    CATATGGTCA AGATCCAGGA TCCGCAGCTG GCAAAACGTT ACTCGATCAA GACTTTCCCG
    GCGTTGGTTT ATTTCAGAAA TGGAAACCCA TTACTGTTTG AGGGGGATCT TCAGAACGAG
    CAATCAGTAT TGGAATGGCT CATCGATGAT GACAACCGCG AGCTGGCTGA TGAAATCGAG
    GAGGTCAACG AGCGTATGCT GGATCGCCTA ATGGCGGAGT CCACTCTATT GGTTGTTTTC
    TTTTACGACG ATGATTGTGC TGAGTGCGAG GAGATTTTGG AAGAGCTGGA GGAGATCGAT
    GGTGAGGCAG ACATGTTCGG CATTGATTTC GTAAAGATTG CTAGTATCCA GGCAGCCAAG
    AAATACGAGA TAGTGAATAT ACCTTCCCTC GTTTATTTCC GAAAACAAGT GCCCGTCCTC
    TACGACGGCG ATCTACACCA ACACGACAAG GTGATTACCT GGCTAACATC ACAGGACGTA
    TTCGAAATCA AAAACGAAAT AGAAGAAGTA AACCGAAAAA TGCTCGACAA ACTACTCGAG
    GAAAATGAGT TCTTAGCCGT TTTTTTCTAC GAACACAATC AGCCGGACAG TACTGCAGCG
    CTGGAAAAAC TCGAAAACAT CGACAGCGAG ACGGATAACC TGGACATCAC CTTCGTCAAG
    ATGGCCGACT CTCGTTATGC CAAGAAATGG GGCGTCACCA AGCTGCCGGC AATGGTCTAC
    TTCCGCCGTC GGTTTCCCAG CATATATAGG GGTGATCTGT TATCCGAGGA CGAAGTGCTG
    GAGTGGCTAC GCAAGAACCG CTTCCGCCAG CCCGAGCTGA ACATCTTTAT GTACGCTCTG
    ATTGCTCTGG CGGTGGCCTT TGTGGTCTAC ACCGCCTTCC TGCTGCAGTG CTTCAAGCCG
    GCGCCACCGC CTCCTGTCCA GCACCCCAAG CAGTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163066.1
    CDS421..4539
    Translation

    Target ORF information:

    RefSeq Version NM_001169595.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169595.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAACATT GCAAGCAATC AGGACGTCTT CAACTGGATT
    CTCGAACAGA AGGCCGACCA AAGCATTCAA CTCATTAATC GTGACCAACT TTTCGAGTAT
    ATAGGCACCA AAGACTTTTT AGCGGTTGTT TTTTACAAAG AAGATGATCC CGACTCGCCA
    CGAGTGCTGC GGCACATCGA ACTAATCGAC GACGAGGCTG CGGAATATGG CATTTACATA
    GTGAAGATGC ACGACAAACT GATGGCCAAG AAGTACGGCT TCCGGAATCC CCCAGGACTG
    ACGTATTTCC GAAAGGGCAA GTATATCAAC TACGACGGCG ATATCGATGA CGAGGAGGAG
    GTACTGGACT GGCTAACGAG CCCGGCCAAC ATGGAGATGA CCGATCACAT CGAGCAGGTT
    AACCGCAAGA TGTTCGAGAA GATCCGCAAG AACTCCGACT ACGTAGCGGT GATATTCTAT
    AGCGATGAGT GCAAGCAGTG TCCTCGCGTC CTGGCCGAGG TGGAACACAT AGACGACGAA
    GCGGACAAGG CTGGTATCGA CTTCGTCAAA ATCGACGATA AGCAGATGGC CAAGGAGTAC
    GGAGTGTTCG CCCTGCCTGC CATTGTCTTC TTCAAGCCCA CATCCAAGGA GCCAGTTATA
    TACGCCGGTG ATCTTTACGA AGAAGAACAG ATCCTAACTT GGCTGATCAC GCAAAAGGAT
    CCAAGTGGAG ATGTTATCGA AGATCTCGAA GGCGAAAGAC TTGTTCATCT GATTGAAGAG
    TCTGGCTCCA TCGCAGTCTA CTTTTATGCG GATGGCTGCG AGCAGTGCAC CAAGGTTTTG
    GAGGAGTTGG AAAACATCGA TGATGATTGC GACAAGCATG GCATAACATT CGTTAAGACC
    AGGGACTTCT CTGTGGCCGA CGGCTATGGC GTGCACGAGT ATCCGGCTCT AGTTTACTTC
    GAGGGAGGAA TCCCCAACGT ATTCGAGGGC GAATTGAGCG AGGAGGAAGA GGTTCTTCAG
    TGGCTTATCA CCCAGAAGAC CGAAGATCGT ATTGAGCTGA TCACTCGCCA GATGCTGGAG
    ACAATGGTGG AGGAGACGCA GTACCTAGCC GTGTATTTCT ACAAAATCAA CTGCAACATA
    TGCGACCAGA TACTAGAGGG CTTGGAACTG ATCGATGACG AATGCGACGT GTTCGGAATT
    CATATGGTCA AGATCCAGGA TCCGCAGCTG GCAAAACGTT ACTCGATCAA GACTTTCCCG
    GCGTTGGTTT ATTTCAGAAA TGGAAACCCA TTACTGTTTG AGGGGGATCT TCAGAACGAG
    CAATCAGTAT TGGAATGGCT CATCGATGAT GACAACCGCG AGCTGGCTGA TGAAATCGAG
    GAGGTCAACG AGCGTATGCT GGATCGCCTA ATGGCGGAGT CCACTCTATT GGTTGTTTTC
    TTTTACGACG ATGATTGTGC TGAGTGCGAG GAGATTTTGG AAGAGCTGGA GGAGATCGAT
    GGTGAGGCAG ACATGTTCGG CATTGATTTC GTAAAGATTG CTAGTATCCA GGCAGCCAAG
    AAATACGAGA TAGTGAATAT ACCTTCCCTC GTTTATTTCC GAAAACAAGT GCCCGTCCTC
    TACGACGGCG ATCTACACCA ACACGACAAG GTGATTACCT GGCTAACATC ACAGGACGTA
    TTCGAAATCA AAAACGAAAT AGAAGAAGTA AACCGAAAAA TGCTCGACAA ACTACTCGAG
    GAAAATGAGT TCTTAGCCGT TTTTTTCTAC GAACACAATC AGCCGGACAG TACTGCAGCG
    CTGGAAAAAC TCGAAAACAT CGACAGCGAG ACGGATAACC TGGACATCAC CTTCGTCAAG
    ATGGCCGACT CTCGTTATGC CAAGAAATGG GGCGTCACCA AGCTGCCGGC AATGGTCTAC
    TTCCGCCGTC GGTTTCCCAG CATATATAGG GGTGATCTGT TATCCGAGGA CGAAGTGCTG
    GAGTGGCTAC GCAAGAACCG CTTCCGCCAG CCCGAGCTGA ACATCTTTAT GTACGCTCTG
    ATTGCTCTGG CGGTGGCCTT TGTGGTCTAC ACCGCCTTCC TGCTGCAGTG CTTCAAGCCG
    GCGCCACCGC CTCCTGTCCA GCACCCCAAG CAGTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa11420
    Clone ID Related Accession (Same CDS sequence) NM_001169594.2
    Accession Version NM_001169594.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5361bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169594.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTGT
    CCAGATCATG AAACATGCCC GCCCAGGGTT ATGGACAAAC AGCAATGCCG CAAGTGCGCC
    AAGGCCTTGC AGGAGCTGGA GAATATCGAC GACGAAGCTG ACCAGCTGGG CATCGGGTTT
    GTGAAGATAC ACGACGAGGC CTTGGCCGAC GAATACAATC TAGGCAATCT GCCAGCCTTG
    GTCTATTACC GCCACCAGAC TCCGATCATA TACGAAGGTG AACTTCAGCG GGAGGAGGAC
    GTCTTGGAAT GGTTGGTGCA GAATAAGTCG ACGGGCGATG AAGATGATGT GATTGAAGAC
    GTCACTTCGA AGACTCTGTC GACGCTGATC AGCAATATCG ACAATCTGGT TGTGCTGTTT
    TATGATCATG GAAACGACGA CTCGATGACC GTGTTAGAGG AGCTAGAGCA AATCGACGAC
    GACTGCGACA AGCATGGCAT TCAGTTTGTG AAAATCGACG ATGCCAAGGC GGCAGCCGAT
    TACGGAATCG ATTCGATTCC GGCCATTGTT TACTTTGAAA AAGAAATTCC AAATGTGTAC
    GACGGCGATC TCATGGACGA GGAGCAGATT CTGAAATGGT TGTTGGGACA GTTGGAACGG
    GATGAGATCG AGGACGTCAC CGACGAAATG CTCGATACAA TGATCAAAGA AGGACGCGTC
    ATTGCCGTGC TGTTCTACGA CAACAACGAC AAGAAGTCCC AGAAAGTGCT CGAGGAGCTG
    GAGAACATTG ACGACGAGTG CGACGCATTG GGCATTACTT TCGTGAAGAT CGACAATCCC
    GAGGAGGCCG TTGAATATGG CATCAATAAA GTTCCTAAAC TGATATACTT TGAAAAAGGC
    ATTCCAACTA TTTACGAGGG CAATCTGGAG GACGAGGAGA AGCTTCTGAA ATGGCTAACA
    GACCAAACGA GTTCCGATCA AATCGAGGAC ATCACCGACG AAATGTTGGA CTTAATCATT
    GAGAAAATGC CCCATGTTGC TGTTCTTTTC TACGACAAAG ACCAAAAGAA ATCACAGAAA
    ATCCTCGCAG AACTGGAAAA CATCGACGAT GAGTGCGATC AGAACGATAT TGCCTTTGTC
    AAGATCGATG ATGACAAGGA GGCCAAAGAA TGGGGTATCG ATGAGATACC ATCGATTGTA
    CTCTTTGAAC GTGGAATTCC ACACATCTAC GAGGGTGATC TGATGAAAGA GGATGAGCTG
    CTTGGCTGGT TGGTGCACCA GAAGCGCTAT TCCGAAATTC CCGAGGTCAC CGATGAGATG
    AAGGACAAGT TGGTCGAGAA CACCGAGCAC TTGGCGGTTA TATTCTACGA CAAGGACGAT
    AAGCAGGATA TGCGCATCCT GAACGAACTG GAGAACATTG ATGACGAGCT GGAGAAGGAG
    GGAATTGTCA TCGTCCGCAT TGATAACGCC GCTGAGGCCA AGGAATATGG TCTCGATCAT
    TTGCCCGCCC TCATCTACTT CGAAAACAAG ATCCCGGCCC TCTATGAAGG CGATCTGATG
    AACGAGGATG AGGTGCTCGA GTGGCTTCTT GTCCAGAAAA AGACAGCTAC TATCGAGGAG
    GTTACCGATG AGATCCTGGT CACTCTGATC AACGAACACG AATATGTCGT CGTCTTCTTT
    ACGGGTCCCT GCGAGCCCGG AGAGACCTGT GAGCACACTC TGAACGCCCT GGAAAGCATC
    GACGATGAAT TGGATGAGGC TGGCATAATT TTTGTTACCA CTGAGGATAC CGGAATTGCC
    AAGAAATACA ACGTCAAGAC CTATCCACGT CTGGTGTTCT TCAGGAATCG TGATCCACTT
    CACTTTACCG GTGATCTGGA CGACGAGGAC GAGGTGTTGG CCTGGATTAC TGACGACGAG
    ACCCTTGAAA TTCCCGGAAA AATTGAGGAG GTCAATGTGA AGATGTTGGA CAAGATCTTG
    GCTGAAAACG ATCACGTTGT CGTATTCTTC TACGCTGAGG GCGATAAGAA GGCCCAAAAG
    ATCCTTAACG AGCTGGAGAA CATCGATGAC GAATGCGAGG AAAAAGACAT TGACTTTGTA
    AAGACATCCG ACGACGATAT TGATAAGGAG TACGACCTGC CCGGTCTGCC GGCACTTGCA
    TTTTATAGAC ATAAGTTTAG AACAATTTAC ACCGGTGACC TGATGAAGGA AGAGGAAATT
    CTCGAGTGGG TTATTGATTT GCACGAGTCC ACAGCTGATG TCATTGAATC TGTCGATCGT
    AAGACCCTGC AAGTTTTGAT CAACGATGTT GAGCACCTGG CTGTGTTCTT CTACGACGAT
    GAATGCGAAT CGTGTTCCGA CATCTTGGAG GAGTTGGAGA ACATCGACGA TGACACCGAC
    AAGCACGGAA TACAATTTGT CAAATCAAAT GATGTTAAGC TGGCTCATGA AATTGGCATT
    TTCGCATTTC CAGCTTTGGT CTACTACGAG ACCGGCGTCC CGATTATGTA TGATGGTAAC
    ATTGCAAGCA ATCAGGACGT CTTCAACTGG ATTCTCGAAC AGAAGGCCGA CCAAAGCATT
    CAACTCATTA ATCGTGACCA ACTTTTCGAG TATATAGGCA CCAAAGACTT TTTAGCGGTT
    GTTTTTTACA AAGAAGATGA TCCCGACTCG CCACGAGTGC TGCGGCACAT CGAACTAATC
    GACGACGAGG CTGCGGAATA TGGCATTTAC ATAGTGAAGA TGCACGACAA ACTGATGGCC
    AAGAAGTACG GCTTCCGGAA TCCCCCAGGA CTGACGTATT TCCGAAAGGG CAAGTATATC
    AACTACGACG GCGATATCGA TGACGAGGAG GAGGTACTGG ACTGGCTAAC GAGCCCGGCC
    AACATGGAGA TGACCGATCA CATCGAGCAG GTTAACCGCA AGATGTTCGA GAAGATCCGC
    AAGAACTCCG ACTACGTAGC GGTGATATTC TATAGCGATG AGTGCAAGCA GTGTCCTCGC
    GTCCTGGCCG AGGTGGAACA CATAGACGAC GAAGCGGACA AGGCTGGTAT CGACTTCGTC
    AAAATCGACG ATAAGCAGAT GGCCAAGGAG TACGGAGTGT TCGCCCTGCC TGCCATTGTC
    TTCTTCAAGC CCACATCCAA GGAGCCAGTT ATATACGCCG GTGATCTTTA CGAAGAAGAA
    CAGATCCTAA CTTGGCTGAT CACGCAAAAG GATCCAAGTG GAGATGTTAT CGAAGATCTC
    GAAGGCGAAA GACTTGTTCA TCTGATTGAA GAGTCTGGCT CCATCGCAGT CTACTTTTGG
    AACAAGACCA AGTGCGACAT TTGTAATTCG AAAGCGGCTC GCAAGGCGCG GCTGAAAAAG
    GAGCGCGATC AGCACCAGCA GGAGGGCGGA GCAGCCAGCG CCGCTGCCGC CTTCGGCAGT
    GAGGCGGATC CTTCCGAGGC GGCCGCTGGT GGGGCGGAGG ATGCGCCAGC GGCAGGTTCT
    GAGGGGGATT CCCCGCCAGC CTCTGCAGCT GCTCCAGCGG ATGCTTCAAC TGGCAAGCAA
    GAGGATGATG CGGATGGCTG CGAGCAGTGC ACCAAGGTTT TGGAGGAGTT GGAAAACATC
    GATGATGATT GCGACAAGCA TGGCATAACA TTCGTTAAGA CCAGGGACTT CTCTGTGGCC
    GACGGCTATG GCGTGCACGA GTATCCGGCT CTAGTTTACT TCGAGGGAGG AATCCCCAAC
    GTATTCGAGG GCGAATTGAG CGAGGAGGAA GAGGTTCTTC AGTGGCTTAT CACCCAGAAG
    ACCGAAGATC GTATTGAGCT GATCACTCGC CAGATGCTGG AGACAATGGT GGAGGAGACG
    CAGTACCTAG CCGTGTATTT CTACAAAATC AACTGCAACA TATGCGACCA GATACTAGAG
    GGCTTGGAAC TGATCGATGA CGAATGCGAC GTGTTCGGAA TTCATATGGT CAAGATCCAG
    GATCCGCAGC TGGCAAAACG TTACTCGATC AAGACTTTCC CGGCGTTGGT TTATTTCAGA
    AATGGAAACC CATTACTGTT TGAGGGGGAT CTTCAGAACG AGCAATCAGT ATTGGAATGG
    CTCATCGATG ATGACAACCG CGAGCTGGCT GATGAAATCG AGGAGGTCAA CGAGCGTATG
    CTGGATCGCC TAATGGCGGA GTCCACTCTA TTGGTTGTTT TCTTTTACGA CGATGATTGT
    GCTGAGTGCG AGGAGATTTT GGAAGAGCTG GAGGAGATCG ATGGTGAGGC AGACATGTTC
    GGCATTGATT TCGTAAAGAT TGCTAGTATC CAGGCAGCCA AGAAATACGA GATAGTGAAT
    ATACCTTCCC TCGTTTATTT CCGAAAACAA GTGCCCGTCC TCTACGACGG CGATCTACAC
    CAACACGACA AGGTGATTAC CTGGCTAACA TCACAGGACG TATTCGAAAT CAAAAACGAA
    ATAGAAGAAG TAAACCGAAA AATGCTCGAC AAACTACTCG AGGAAAATGA GTTCTTAGCC
    GTTTTTTTCT ACGAACACAA TCAGCCGGAC AGTACTGCAG CGCTGGAAAA ACTCGAAAAC
    ATCGACAGCG AGACGGATAA CCTGGACATC ACCTTCGTCA AGATGGCCGA CTCTCGTTAT
    GCCAAGAAAT GGGGCGTCAC CAAGCTGCCG GCAATGGTCT ACTTCCGCCG TCGGTTTCCC
    AGCATATATA GGGGTGATCT GTTATCCGAG GACGAAGTGC TGGAGTGGCT ACGCAAGAAC
    CGCTTCCGCC AGCCCGAGCT GAACATCTTT ATGTACGCTC TGATTGCTCT GGCGGTGGCC
    TTTGTGGTCT ACACCGCCTT CCTGCTGCAG TGCTTCAAGC CGGCGCCACC GCCTCCTGTC
    CAGCACCCCA AGCAGTCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163065.1
    CDS421..5781
    Translation

    Target ORF information:

    RefSeq Version NM_001169594.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169594.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTGT
    CCAGATCATG AAACATGCCC GCCCAGGGTT ATGGACAAAC AGCAATGCCG CAAGTGCGCC
    AAGGCCTTGC AGGAGCTGGA GAATATCGAC GACGAAGCTG ACCAGCTGGG CATCGGGTTT
    GTGAAGATAC ACGACGAGGC CTTGGCCGAC GAATACAATC TAGGCAATCT GCCAGCCTTG
    GTCTATTACC GCCACCAGAC TCCGATCATA TACGAAGGTG AACTTCAGCG GGAGGAGGAC
    GTCTTGGAAT GGTTGGTGCA GAATAAGTCG ACGGGCGATG AAGATGATGT GATTGAAGAC
    GTCACTTCGA AGACTCTGTC GACGCTGATC AGCAATATCG ACAATCTGGT TGTGCTGTTT
    TATGATCATG GAAACGACGA CTCGATGACC GTGTTAGAGG AGCTAGAGCA AATCGACGAC
    GACTGCGACA AGCATGGCAT TCAGTTTGTG AAAATCGACG ATGCCAAGGC GGCAGCCGAT
    TACGGAATCG ATTCGATTCC GGCCATTGTT TACTTTGAAA AAGAAATTCC AAATGTGTAC
    GACGGCGATC TCATGGACGA GGAGCAGATT CTGAAATGGT TGTTGGGACA GTTGGAACGG
    GATGAGATCG AGGACGTCAC CGACGAAATG CTCGATACAA TGATCAAAGA AGGACGCGTC
    ATTGCCGTGC TGTTCTACGA CAACAACGAC AAGAAGTCCC AGAAAGTGCT CGAGGAGCTG
    GAGAACATTG ACGACGAGTG CGACGCATTG GGCATTACTT TCGTGAAGAT CGACAATCCC
    GAGGAGGCCG TTGAATATGG CATCAATAAA GTTCCTAAAC TGATATACTT TGAAAAAGGC
    ATTCCAACTA TTTACGAGGG CAATCTGGAG GACGAGGAGA AGCTTCTGAA ATGGCTAACA
    GACCAAACGA GTTCCGATCA AATCGAGGAC ATCACCGACG AAATGTTGGA CTTAATCATT
    GAGAAAATGC CCCATGTTGC TGTTCTTTTC TACGACAAAG ACCAAAAGAA ATCACAGAAA
    ATCCTCGCAG AACTGGAAAA CATCGACGAT GAGTGCGATC AGAACGATAT TGCCTTTGTC
    AAGATCGATG ATGACAAGGA GGCCAAAGAA TGGGGTATCG ATGAGATACC ATCGATTGTA
    CTCTTTGAAC GTGGAATTCC ACACATCTAC GAGGGTGATC TGATGAAAGA GGATGAGCTG
    CTTGGCTGGT TGGTGCACCA GAAGCGCTAT TCCGAAATTC CCGAGGTCAC CGATGAGATG
    AAGGACAAGT TGGTCGAGAA CACCGAGCAC TTGGCGGTTA TATTCTACGA CAAGGACGAT
    AAGCAGGATA TGCGCATCCT GAACGAACTG GAGAACATTG ATGACGAGCT GGAGAAGGAG
    GGAATTGTCA TCGTCCGCAT TGATAACGCC GCTGAGGCCA AGGAATATGG TCTCGATCAT
    TTGCCCGCCC TCATCTACTT CGAAAACAAG ATCCCGGCCC TCTATGAAGG CGATCTGATG
    AACGAGGATG AGGTGCTCGA GTGGCTTCTT GTCCAGAAAA AGACAGCTAC TATCGAGGAG
    GTTACCGATG AGATCCTGGT CACTCTGATC AACGAACACG AATATGTCGT CGTCTTCTTT
    ACGGGTCCCT GCGAGCCCGG AGAGACCTGT GAGCACACTC TGAACGCCCT GGAAAGCATC
    GACGATGAAT TGGATGAGGC TGGCATAATT TTTGTTACCA CTGAGGATAC CGGAATTGCC
    AAGAAATACA ACGTCAAGAC CTATCCACGT CTGGTGTTCT TCAGGAATCG TGATCCACTT
    CACTTTACCG GTGATCTGGA CGACGAGGAC GAGGTGTTGG CCTGGATTAC TGACGACGAG
    ACCCTTGAAA TTCCCGGAAA AATTGAGGAG GTCAATGTGA AGATGTTGGA CAAGATCTTG
    GCTGAAAACG ATCACGTTGT CGTATTCTTC TACGCTGAGG GCGATAAGAA GGCCCAAAAG
    ATCCTTAACG AGCTGGAGAA CATCGATGAC GAATGCGAGG AAAAAGACAT TGACTTTGTA
    AAGACATCCG ACGACGATAT TGATAAGGAG TACGACCTGC CCGGTCTGCC GGCACTTGCA
    TTTTATAGAC ATAAGTTTAG AACAATTTAC ACCGGTGACC TGATGAAGGA AGAGGAAATT
    CTCGAGTGGG TTATTGATTT GCACGAGTCC ACAGCTGATG TCATTGAATC TGTCGATCGT
    AAGACCCTGC AAGTTTTGAT CAACGATGTT GAGCACCTGG CTGTGTTCTT CTACGACGAT
    GAATGCGAAT CGTGTTCCGA CATCTTGGAG GAGTTGGAGA ACATCGACGA TGACACCGAC
    AAGCACGGAA TACAATTTGT CAAATCAAAT GATGTTAAGC TGGCTCATGA AATTGGCATT
    TTCGCATTTC CAGCTTTGGT CTACTACGAG ACCGGCGTCC CGATTATGTA TGATGGTAAC
    ATTGCAAGCA ATCAGGACGT CTTCAACTGG ATTCTCGAAC AGAAGGCCGA CCAAAGCATT
    CAACTCATTA ATCGTGACCA ACTTTTCGAG TATATAGGCA CCAAAGACTT TTTAGCGGTT
    GTTTTTTACA AAGAAGATGA TCCCGACTCG CCACGAGTGC TGCGGCACAT CGAACTAATC
    GACGACGAGG CTGCGGAATA TGGCATTTAC ATAGTGAAGA TGCACGACAA ACTGATGGCC
    AAGAAGTACG GCTTCCGGAA TCCCCCAGGA CTGACGTATT TCCGAAAGGG CAAGTATATC
    AACTACGACG GCGATATCGA TGACGAGGAG GAGGTACTGG ACTGGCTAAC GAGCCCGGCC
    AACATGGAGA TGACCGATCA CATCGAGCAG GTTAACCGCA AGATGTTCGA GAAGATCCGC
    AAGAACTCCG ACTACGTAGC GGTGATATTC TATAGCGATG AGTGCAAGCA GTGTCCTCGC
    GTCCTGGCCG AGGTGGAACA CATAGACGAC GAAGCGGACA AGGCTGGTAT CGACTTCGTC
    AAAATCGACG ATAAGCAGAT GGCCAAGGAG TACGGAGTGT TCGCCCTGCC TGCCATTGTC
    TTCTTCAAGC CCACATCCAA GGAGCCAGTT ATATACGCCG GTGATCTTTA CGAAGAAGAA
    CAGATCCTAA CTTGGCTGAT CACGCAAAAG GATCCAAGTG GAGATGTTAT CGAAGATCTC
    GAAGGCGAAA GACTTGTTCA TCTGATTGAA GAGTCTGGCT CCATCGCAGT CTACTTTTGG
    AACAAGACCA AGTGCGACAT TTGTAATTCG AAAGCGGCTC GCAAGGCGCG GCTGAAAAAG
    GAGCGCGATC AGCACCAGCA GGAGGGCGGA GCAGCCAGCG CCGCTGCCGC CTTCGGCAGT
    GAGGCGGATC CTTCCGAGGC GGCCGCTGGT GGGGCGGAGG ATGCGCCAGC GGCAGGTTCT
    GAGGGGGATT CCCCGCCAGC CTCTGCAGCT GCTCCAGCGG ATGCTTCAAC TGGCAAGCAA
    GAGGATGATG CGGATGGCTG CGAGCAGTGC ACCAAGGTTT TGGAGGAGTT GGAAAACATC
    GATGATGATT GCGACAAGCA TGGCATAACA TTCGTTAAGA CCAGGGACTT CTCTGTGGCC
    GACGGCTATG GCGTGCACGA GTATCCGGCT CTAGTTTACT TCGAGGGAGG AATCCCCAAC
    GTATTCGAGG GCGAATTGAG CGAGGAGGAA GAGGTTCTTC AGTGGCTTAT CACCCAGAAG
    ACCGAAGATC GTATTGAGCT GATCACTCGC CAGATGCTGG AGACAATGGT GGAGGAGACG
    CAGTACCTAG CCGTGTATTT CTACAAAATC AACTGCAACA TATGCGACCA GATACTAGAG
    GGCTTGGAAC TGATCGATGA CGAATGCGAC GTGTTCGGAA TTCATATGGT CAAGATCCAG
    GATCCGCAGC TGGCAAAACG TTACTCGATC AAGACTTTCC CGGCGTTGGT TTATTTCAGA
    AATGGAAACC CATTACTGTT TGAGGGGGAT CTTCAGAACG AGCAATCAGT ATTGGAATGG
    CTCATCGATG ATGACAACCG CGAGCTGGCT GATGAAATCG AGGAGGTCAA CGAGCGTATG
    CTGGATCGCC TAATGGCGGA GTCCACTCTA TTGGTTGTTT TCTTTTACGA CGATGATTGT
    GCTGAGTGCG AGGAGATTTT GGAAGAGCTG GAGGAGATCG ATGGTGAGGC AGACATGTTC
    GGCATTGATT TCGTAAAGAT TGCTAGTATC CAGGCAGCCA AGAAATACGA GATAGTGAAT
    ATACCTTCCC TCGTTTATTT CCGAAAACAA GTGCCCGTCC TCTACGACGG CGATCTACAC
    CAACACGACA AGGTGATTAC CTGGCTAACA TCACAGGACG TATTCGAAAT CAAAAACGAA
    ATAGAAGAAG TAAACCGAAA AATGCTCGAC AAACTACTCG AGGAAAATGA GTTCTTAGCC
    GTTTTTTTCT ACGAACACAA TCAGCCGGAC AGTACTGCAG CGCTGGAAAA ACTCGAAAAC
    ATCGACAGCG AGACGGATAA CCTGGACATC ACCTTCGTCA AGATGGCCGA CTCTCGTTAT
    GCCAAGAAAT GGGGCGTCAC CAAGCTGCCG GCAATGGTCT ACTTCCGCCG TCGGTTTCCC
    AGCATATATA GGGGTGATCT GTTATCCGAG GACGAAGTGC TGGAGTGGCT ACGCAAGAAC
    CGCTTCCGCC AGCCCGAGCT GAACATCTTT ATGTACGCTC TGATTGCTCT GGCGGTGGCC
    TTTGTGGTCT ACACCGCCTT CCTGCTGCAG TGCTTCAAGC CGGCGCCACC GCCTCCTGTC
    CAGCACCCCA AGCAGTCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12722
    Clone ID Related Accession (Same CDS sequence) NM_001043010.4
    Accession Version NM_001043010.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform K
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001043010.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    AAACAGCAAT GCCGCAAGTG CGCCAAGGCC TTGCAGGAGC TGGAGAATAT CGACGACGAA
    GCTGACCAGC TGGGCATCGG GTTTGTGAAG ATACACGACG AGGCCTTGGC CGACGAATAC
    AATCTAGGCA ATCTGCCAGC CTTGGTCTAT TACCGCCACC AGACTCCGAT CATATACGAA
    GGTGAACTTC AGCGGGAGGA GGACGTCTTG GAATGGTTGG TGCAGAATAA GTCGACGGGC
    GATGAAGATG ATGTGATTGA AGACGTCACT TCGAAGACTC TGTCGACGCT GATCAGCAAT
    ATCGACAATC TGGTTGTGCT GTTTTATGAT CATGGAAACG ACGACTCGAT GACCGTGTTA
    GAGGAGCTAG AGCAAATCGA CGACGACTGC GACAAGCATG GCATTCAGTT TGTGAAAATC
    GACGATGCCA AGGCGGCAGC CGATTACGGA ATCGATTCGA TTCCGGCCAT TGTTTACTTT
    GAAAAAGAAA TTCCAAATGT GTACGACGGC GATCTCATGG ACGAGGAGCA GATTCTGAAA
    TGGTTGTTGG GACAGTTGGA ACGGGATGAG ATCGAGGACG TCACCGACGA AATGCTCGAT
    ACAATGATCA AAGAAGGACG CGTCATTGCC GTGCTGTTCT ACGACAACAA CGACAAGAAG
    TCCCAGAAAG TGCTCGAGGA GCTGGAGAAC ATTGACGACG AGTGCGACGC ATTGGGCATT
    ACTTTCGTGA AGATCGACAA TCCCGAGGAG GCCGTTGAAT ATGGCATCAA TAAAGTTCCT
    AAACTGATAT ACTTTGAAAA AGGCATTCCA ACTATTTACG AGGGCAATCT GGAGGACGAG
    GAGAAGCTTC TGAAATGGCT AACAGACCAA ACGAGTTCCG ATCAAATCGA GGACATCACC
    GACGAAATGT TGGACTTAAT CATTGAGAAA ATGCCCCATG TTGCTGTTCT TTTCTACGAC
    AAAGACCAAA AGAAATCACA GAAAATCCTC GCAGAACTGG AAAACATCGA CGATGAGTGC
    GATCAGAACG ATATTGCCTT TGTCAAGATC GATGATGACA AGGAGGCCAA AGAATGGGGT
    ATCGATGAGA TACCATCGAT TGTACTCTTT GAACGTGGAA TTCCACACAT CTACGAGGGT
    GATCTGATGA AAGAGGATGA GCTGCTTGGC TGGTTGGTGC ACCAGAAGCG CTATTCCGAA
    ATTCCCGAGG TCACCGATGA GATGAAGGAC AAGTTGGTCG AGAACACCGA GCACTTGGCG
    GTTATATTCT ACGACAAGGA CGATAAGCAG GATATGCGCA TCCTGAACGA ACTGGAGAAC
    ATTGATGACG AGCTGGAGAA GGAGGGAATT GTCATCGTCC GCATTGATAA CGCCGCTGAG
    GCCAAGGAAT ATGGTCTCGA TCATTTGCCC GCCCTCATCT ACTTCGAAAA CAAGATCCCG
    GCCCTCTATG AAGGCGATCT GATGAACGAG GATGAGGTGC TCGAGTGGCT TCTTGTCCAG
    AAAAAGACAG CTACTATCGA GGAGGTTACC GATGAGATCC TGGTCACTCT GATCAACGAA
    CACGAATATG TCGTCGTCTT CTTTACGGGT CCCTGCGAGC CCGGAGAGAC CTGTGAGCAC
    ACTCTGAACG CCCTGGAAAG CATCGACGAT GAATTGGATG AGGCTGGCAT AATTTTTGTT
    ACCACTGAGG ATACCGGAAT TGCCAAGAAA TACAACGTCA AGACCTATCC ACGTCTGGTG
    TTCTTCAGGA ATCGTGATCC ACTTCACTTT ACCGGTGATC TGGACGACGA GGACGAGGTG
    TTGGCCTGGA TTACTGACGA CGAGACCCTT GAAATTCCCG GAAAAATTGA GGAGGTCAAT
    GTGAAGATGT TGGACAAGAT CTTGGCTGAA AACGATCACG TTGTCGTATT CTTCTACGCT
    GAGGGCGATA AGAAGGCCCA AAAGATCCTT AACGAGCTGG AGAACATCGA TGACGAATGC
    GAGGAAAAAG ACATTGACTT TGTAAAGACA TCCGACGACG ATATTGATAA GGAGTACGAC
    CTGCCCGGTC TGCCGGCACT TGCATTTTAT AGACATAAGT TTAGAACAAT TTACACCGGT
    GACCTGATGA AGGAAGAGGA AATTCTCGAG TGGGTTATTG ATTTGCACGA GTCCACAGCT
    GATGTCATTG AATCTGTCGA TCGTAAGACC CTGCAAGTTT TGATCAACGA TGTTGAGCAC
    CTGGCTGTGT TCTTCTACGA CGATGAATGC GAATCGTGTT CCGACATCTT GGAGGAGTTG
    GAGAACATCG ACGATGACAC CGACAAGCAC GGAATACAAT TTGTCAAATC AAATGATGTT
    AAGCTGGCTC ATGAAATTGG CATTTTCGCA TTTCCAGCTT TGGTCTACTA CGAGACCGGC
    GTCCCGATTA TGTATGATGG TAACATTGCA AGCAATCAGG ACGTCTTCAA CTGGATTCTC
    GAACAGAAGG CCGACCAAAG CATTCAACTC ATTAATCGTG ACCAACTTTT CGAGTATATA
    GGCACCAAAG ACTTTTTAGC GGTTGTTTTT TACAAAGAAG ATGATCCCGA CTCGCCACGA
    GTGCTGCGGC ACATCGAACT AATCGACGAC GAGGCTGCGG AATATGGCAT TTACATAGTG
    AAGATGCACG ACAAACTGAT GGCCAAGAAG TACGGCTTCC GGAATCCCCC AGGACTGACG
    TATTTCCGAA AGGGCAAGTA TATCAACTAC GACGGCGATA TCGATGACGA GGAGGAGGTA
    CTGGACTGGC TAACGAGCCC GGCCAACATG GAGATGACCG ATCACATCGA GCAGGTTAAC
    CGCAAGATGT TCGAGAAGAT CCGCAAGAAC TCCGACTACG TAGCGGTGAT ATTCTATAGC
    GATGAGTGCA AGCAGTGTCC TCGCGTCCTG GCCGAGGTGG AACACATAGA CGACGAAGCG
    GACAAGGCTG GTATCGACTT CGTCAAAATC GACGATAAGC AGATGGCCAA GGAGTACGGA
    GTGTTCGCCC TGCCTGCCAT TGTCTTCTTC AAGCCCACAT CCAAGGAGCC AGTTATATAC
    GCCGGTGATC TTTACGAAGA AGAACAGATC CTAACTTGGC TGATCACGCA AAAGGATCCA
    AGTGGAGATG TTATCGAAGA TCTCGAAGGC GAAAGACTTG TTCATCTGAT TGAAGAGTCT
    GGCTCCATCG CAGTCTACTT TTATGCGGAT GGCTGCGAGC AGTGCACCAA GGTTTTGGAG
    GAGTTGGAAA ACATCGATGA TGATTGCGAC AAGCATGGCA TAACATTCGT TAAGACCAGG
    GACTTCTCTG TGGCCGACGG CTATGGCGTG CACGAGTATC CGGCTCTAGT TTACTTCGAG
    GGAGGAATCC CCAACGTATT CGAGGGCGAA TTGAGCGAGG AGGAAGAGGT TCTTCAGTGG
    CTTATCACCC AGAAGACCGA AGATCGTATT GAGCTGATCA CTCGCCAGAT GCTGGAGACA
    ATGGTGGAGG AGACGCAGTA CCTAGCCGTG TATTTCTTGC CACCAGAGCG CAATGGGAAG
    CAACCCGCTT TCTGCCGCAG TTTCTGCTCC CCCTCTAGTC TTAAAGAAAG CAACCTGACC
    ACCACCAAAT CCACAAAACA CTCATCCAGC CAATTCGTAC AAAACTACAT CTCACGTAAA
    AGAAAACGTA TCGAAAAACA AGACAAAATC AACTGCAACA TATGCGACCA GATACTAGAG
    GGCTTGGAAC TGATCGATGA CGAATGCGAC GTGTTCGGAA TTCATATGGT CAAGATCCAG
    GATCCGCAGC TGGCAAAACG TTACTCGATC AAGACTTTCC CGGCGTTGGT TTATTTCAGA
    AATGGAAACC CATTACTGTT TGAGGGGGAT CTTCAGAACG AGCAATCAGT ATTGGAATGG
    CTCATCGATG ATGACAACCG CGAGCTGGCT GATGAAATCG AGGAGGTCAA CGAGCGTATG
    CTGGATCGCC TAATGGCGGA GTCCACTCTA TTGGTTGTTT TCTTTTACGA CGATGATTGT
    GCTGAGTGCG AGGAGATTTT GGAAGAGCTG GAGGAGATCG ATGGTGAGGC AGACATGTTC
    GGCATTGATT TCGTAAAGAT TGCTAGTATC CAGGCAGCCA AGAAATACGA GATAGTGAAT
    ATACCTTCCC TCGTTTATTT CCGAAAACAA GTGCCCGTCC TCTACGACGG CGATCTACAC
    CAACACGACA AGGTGATTAC CTGGCTAACA TCACAGGACG TATTCGAAAT CAAAAACGAA
    ATAGAAGAAG TAAACCGAAA AATGCTCGAC AAACTACTCG AGGAAAATGA GTTCTTAGCC
    GTTTTTTTCT ACGAACACAA TCAGCCGGAC AGTACTGCAG CGCTGGAAAA ACTCGAAAAC
    ATCGACAGCG AGACGGATAA CCTGGACATC ACCTTCGTCA AGATGGCCGA CTCTCGTTAT
    GCCAAGAAAT GGGGCGTCAC CAAGCTGCCG GCAATGGTCT ACTTCCGCCG TCGGTTTCCC
    AGCATATATA GGGGTGATCT GTTATCCGAG GACGAAGTGC TGGAGTGGCT ACGCAAGAAC
    CGCTTCCGCC AGCCCGAGCT GAACATCTTT ATGTACGCTC TGATTGCTCT GGCGGTGGCC
    TTTGTGGTCT ACACCGCCTT CCTGCTGCAG TGCTTCAAGC CGGCGCCACC GCCTCCTGTC
    CAGCACCCCA AGCAGTCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036475.3
    CDS421..5661
    Translation

    Target ORF information:

    RefSeq Version NM_001043010.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant K, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001043010.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    AAACAGCAAT GCCGCAAGTG CGCCAAGGCC TTGCAGGAGC TGGAGAATAT CGACGACGAA
    GCTGACCAGC TGGGCATCGG GTTTGTGAAG ATACACGACG AGGCCTTGGC CGACGAATAC
    AATCTAGGCA ATCTGCCAGC CTTGGTCTAT TACCGCCACC AGACTCCGAT CATATACGAA
    GGTGAACTTC AGCGGGAGGA GGACGTCTTG GAATGGTTGG TGCAGAATAA GTCGACGGGC
    GATGAAGATG ATGTGATTGA AGACGTCACT TCGAAGACTC TGTCGACGCT GATCAGCAAT
    ATCGACAATC TGGTTGTGCT GTTTTATGAT CATGGAAACG ACGACTCGAT GACCGTGTTA
    GAGGAGCTAG AGCAAATCGA CGACGACTGC GACAAGCATG GCATTCAGTT TGTGAAAATC
    GACGATGCCA AGGCGGCAGC CGATTACGGA ATCGATTCGA TTCCGGCCAT TGTTTACTTT
    GAAAAAGAAA TTCCAAATGT GTACGACGGC GATCTCATGG ACGAGGAGCA GATTCTGAAA
    TGGTTGTTGG GACAGTTGGA ACGGGATGAG ATCGAGGACG TCACCGACGA AATGCTCGAT
    ACAATGATCA AAGAAGGACG CGTCATTGCC GTGCTGTTCT ACGACAACAA CGACAAGAAG
    TCCCAGAAAG TGCTCGAGGA GCTGGAGAAC ATTGACGACG AGTGCGACGC ATTGGGCATT
    ACTTTCGTGA AGATCGACAA TCCCGAGGAG GCCGTTGAAT ATGGCATCAA TAAAGTTCCT
    AAACTGATAT ACTTTGAAAA AGGCATTCCA ACTATTTACG AGGGCAATCT GGAGGACGAG
    GAGAAGCTTC TGAAATGGCT AACAGACCAA ACGAGTTCCG ATCAAATCGA GGACATCACC
    GACGAAATGT TGGACTTAAT CATTGAGAAA ATGCCCCATG TTGCTGTTCT TTTCTACGAC
    AAAGACCAAA AGAAATCACA GAAAATCCTC GCAGAACTGG AAAACATCGA CGATGAGTGC
    GATCAGAACG ATATTGCCTT TGTCAAGATC GATGATGACA AGGAGGCCAA AGAATGGGGT
    ATCGATGAGA TACCATCGAT TGTACTCTTT GAACGTGGAA TTCCACACAT CTACGAGGGT
    GATCTGATGA AAGAGGATGA GCTGCTTGGC TGGTTGGTGC ACCAGAAGCG CTATTCCGAA
    ATTCCCGAGG TCACCGATGA GATGAAGGAC AAGTTGGTCG AGAACACCGA GCACTTGGCG
    GTTATATTCT ACGACAAGGA CGATAAGCAG GATATGCGCA TCCTGAACGA ACTGGAGAAC
    ATTGATGACG AGCTGGAGAA GGAGGGAATT GTCATCGTCC GCATTGATAA CGCCGCTGAG
    GCCAAGGAAT ATGGTCTCGA TCATTTGCCC GCCCTCATCT ACTTCGAAAA CAAGATCCCG
    GCCCTCTATG AAGGCGATCT GATGAACGAG GATGAGGTGC TCGAGTGGCT TCTTGTCCAG
    AAAAAGACAG CTACTATCGA GGAGGTTACC GATGAGATCC TGGTCACTCT GATCAACGAA
    CACGAATATG TCGTCGTCTT CTTTACGGGT CCCTGCGAGC CCGGAGAGAC CTGTGAGCAC
    ACTCTGAACG CCCTGGAAAG CATCGACGAT GAATTGGATG AGGCTGGCAT AATTTTTGTT
    ACCACTGAGG ATACCGGAAT TGCCAAGAAA TACAACGTCA AGACCTATCC ACGTCTGGTG
    TTCTTCAGGA ATCGTGATCC ACTTCACTTT ACCGGTGATC TGGACGACGA GGACGAGGTG
    TTGGCCTGGA TTACTGACGA CGAGACCCTT GAAATTCCCG GAAAAATTGA GGAGGTCAAT
    GTGAAGATGT TGGACAAGAT CTTGGCTGAA AACGATCACG TTGTCGTATT CTTCTACGCT
    GAGGGCGATA AGAAGGCCCA AAAGATCCTT AACGAGCTGG AGAACATCGA TGACGAATGC
    GAGGAAAAAG ACATTGACTT TGTAAAGACA TCCGACGACG ATATTGATAA GGAGTACGAC
    CTGCCCGGTC TGCCGGCACT TGCATTTTAT AGACATAAGT TTAGAACAAT TTACACCGGT
    GACCTGATGA AGGAAGAGGA AATTCTCGAG TGGGTTATTG ATTTGCACGA GTCCACAGCT
    GATGTCATTG AATCTGTCGA TCGTAAGACC CTGCAAGTTT TGATCAACGA TGTTGAGCAC
    CTGGCTGTGT TCTTCTACGA CGATGAATGC GAATCGTGTT CCGACATCTT GGAGGAGTTG
    GAGAACATCG ACGATGACAC CGACAAGCAC GGAATACAAT TTGTCAAATC AAATGATGTT
    AAGCTGGCTC ATGAAATTGG CATTTTCGCA TTTCCAGCTT TGGTCTACTA CGAGACCGGC
    GTCCCGATTA TGTATGATGG TAACATTGCA AGCAATCAGG ACGTCTTCAA CTGGATTCTC
    GAACAGAAGG CCGACCAAAG CATTCAACTC ATTAATCGTG ACCAACTTTT CGAGTATATA
    GGCACCAAAG ACTTTTTAGC GGTTGTTTTT TACAAAGAAG ATGATCCCGA CTCGCCACGA
    GTGCTGCGGC ACATCGAACT AATCGACGAC GAGGCTGCGG AATATGGCAT TTACATAGTG
    AAGATGCACG ACAAACTGAT GGCCAAGAAG TACGGCTTCC GGAATCCCCC AGGACTGACG
    TATTTCCGAA AGGGCAAGTA TATCAACTAC GACGGCGATA TCGATGACGA GGAGGAGGTA
    CTGGACTGGC TAACGAGCCC GGCCAACATG GAGATGACCG ATCACATCGA GCAGGTTAAC
    CGCAAGATGT TCGAGAAGAT CCGCAAGAAC TCCGACTACG TAGCGGTGAT ATTCTATAGC
    GATGAGTGCA AGCAGTGTCC TCGCGTCCTG GCCGAGGTGG AACACATAGA CGACGAAGCG
    GACAAGGCTG GTATCGACTT CGTCAAAATC GACGATAAGC AGATGGCCAA GGAGTACGGA
    GTGTTCGCCC TGCCTGCCAT TGTCTTCTTC AAGCCCACAT CCAAGGAGCC AGTTATATAC
    GCCGGTGATC TTTACGAAGA AGAACAGATC CTAACTTGGC TGATCACGCA AAAGGATCCA
    AGTGGAGATG TTATCGAAGA TCTCGAAGGC GAAAGACTTG TTCATCTGAT TGAAGAGTCT
    GGCTCCATCG CAGTCTACTT TTATGCGGAT GGCTGCGAGC AGTGCACCAA GGTTTTGGAG
    GAGTTGGAAA ACATCGATGA TGATTGCGAC AAGCATGGCA TAACATTCGT TAAGACCAGG
    GACTTCTCTG TGGCCGACGG CTATGGCGTG CACGAGTATC CGGCTCTAGT TTACTTCGAG
    GGAGGAATCC CCAACGTATT CGAGGGCGAA TTGAGCGAGG AGGAAGAGGT TCTTCAGTGG
    CTTATCACCC AGAAGACCGA AGATCGTATT GAGCTGATCA CTCGCCAGAT GCTGGAGACA
    ATGGTGGAGG AGACGCAGTA CCTAGCCGTG TATTTCTTGC CACCAGAGCG CAATGGGAAG
    CAACCCGCTT TCTGCCGCAG TTTCTGCTCC CCCTCTAGTC TTAAAGAAAG CAACCTGACC
    ACCACCAAAT CCACAAAACA CTCATCCAGC CAATTCGTAC AAAACTACAT CTCACGTAAA
    AGAAAACGTA TCGAAAAACA AGACAAAATC AACTGCAACA TATGCGACCA GATACTAGAG
    GGCTTGGAAC TGATCGATGA CGAATGCGAC GTGTTCGGAA TTCATATGGT CAAGATCCAG
    GATCCGCAGC TGGCAAAACG TTACTCGATC AAGACTTTCC CGGCGTTGGT TTATTTCAGA
    AATGGAAACC CATTACTGTT TGAGGGGGAT CTTCAGAACG AGCAATCAGT ATTGGAATGG
    CTCATCGATG ATGACAACCG CGAGCTGGCT GATGAAATCG AGGAGGTCAA CGAGCGTATG
    CTGGATCGCC TAATGGCGGA GTCCACTCTA TTGGTTGTTT TCTTTTACGA CGATGATTGT
    GCTGAGTGCG AGGAGATTTT GGAAGAGCTG GAGGAGATCG ATGGTGAGGC AGACATGTTC
    GGCATTGATT TCGTAAAGAT TGCTAGTATC CAGGCAGCCA AGAAATACGA GATAGTGAAT
    ATACCTTCCC TCGTTTATTT CCGAAAACAA GTGCCCGTCC TCTACGACGG CGATCTACAC
    CAACACGACA AGGTGATTAC CTGGCTAACA TCACAGGACG TATTCGAAAT CAAAAACGAA
    ATAGAAGAAG TAAACCGAAA AATGCTCGAC AAACTACTCG AGGAAAATGA GTTCTTAGCC
    GTTTTTTTCT ACGAACACAA TCAGCCGGAC AGTACTGCAG CGCTGGAAAA ACTCGAAAAC
    ATCGACAGCG AGACGGATAA CCTGGACATC ACCTTCGTCA AGATGGCCGA CTCTCGTTAT
    GCCAAGAAAT GGGGCGTCAC CAAGCTGCCG GCAATGGTCT ACTTCCGCCG TCGGTTTCCC
    AGCATATATA GGGGTGATCT GTTATCCGAG GACGAAGTGC TGGAGTGGCT ACGCAAGAAC
    CGCTTCCGCC AGCCCGAGCT GAACATCTTT ATGTACGCTC TGATTGCTCT GGCGGTGGCC
    TTTGTGGTCT ACACCGCCTT CCTGCTGCAG TGCTTCAAGC CGGCGCCACC GCCTCCTGTC
    CAGCACCCCA AGCAGTCTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa12723
    Clone ID Related Accession (Same CDS sequence) NM_165485.3
    Accession Version NM_165485.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165485.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTGT
    CCAGATCATG AAACATGCCC GCCCAGGGTT ATGGACAAAC AGCAATGCCG CAAGTGCGCC
    AAGGCCTTGC AGGAGCTGGA GAATATCGAC GACGAAGCTG ACCAGCTGGG CATCGGGTTT
    GTGAAGATAC ACGACGAGGC CTTGGCCGAC GAATACAATC TAGGCAATCT GCCAGCCTTG
    GTCTATTACC GCCACCAGAC TCCGATCATA TACGAAGGTG AACTTCAGCG GGAGGAGGAC
    GTCTTGGAAT GGTTGGTGCA GAATAAGTCG ACGGGCGATG AAGATGATGT GATTGAAGAC
    GTCACTTCGA AGACTCTGTC GACGCTGATC AGCAATATCG ACAATCTGGT TGTGCTGTTT
    TATGATCATG GAAACGACGA CTCGATGACC GTGTTAGAGG AGCTAGAGCA AATCGACGAC
    GACTGCGACA AGCATGGCAT TCAGTTTGTG AAAATCGACG ATGCCAAGGC GGCAGCCGAT
    TACGGAATCG ATTCGATTCC GGCCATTGTT TACTTTGAAA AAGAAATTCC AAATGTGTAC
    GACGGCGATC TCATGGACGA GGAGCAGATT CTGAAATGGT TGTTGGGACA GTTGGAACGG
    GATGAGATCG AGGACGTCAC CGACGAAATG CTCGATACAA TGATCAAAGA AGGACGCGTC
    ATTGCCGTGC TGTTCTACGA CAACAACGAC AAGAAGTCCC AGAAAGTGCT CGAGGAGCTG
    GAGAACATTG ACGACGAGTG CGACGCATTG GGCATTACTT TCGTGAAGAT CGACAATCCC
    GAGGAGGCCG TTGAATATGG CATCAATAAA GTTCCTAAAC TGATATACTT TGAAAAAGGC
    ATTCCAACTA TTTACGAGGG CAATCTGGAG GACGAGGAGA AGCTTCTGAA ATGGCTAACA
    GACCAAACGA GTTCCGATCA AATCGAGGAC ATCACCGACG AAATGTTGGA CTTAATCATT
    GAGAAAATGC CCCATGTTGC TGTTCTTTTC TACGACAAAG ACCAAAAGAA ATCACAGAAA
    ATCCTCGCAG AACTGGAAAA CATCGACGAT GAGTGCGATC AGAACGATAT TGCCTTTGTC
    AAGATCGATG ATGACAAGGA GGCCAAAGAA TGGGGTATCG ATGAGATACC ATCGATTGTA
    CTCTTTGAAC GTGGAATTCC ACACATCTAC GAGGGTGATC TGATGAAAGA GGATGAGCTG
    CTTGGCTGGT TGGTGCACCA GAAGCGCTAT TCCGAAATTC CCGAGGTCAC CGATGAGATG
    AAGGACAAGT TGGTCGAGAA CACCGAGCAC TTGGCGGTTA TATTCTACGA CAAGGACGAT
    AAGCAGGATA TGCGCATCCT GAACGAACTG GAGAACATTG ATGACGAGCT GGAGAAGGAG
    GGAATTGTCA TCGTCCGCAT TGATAACGCC GCTGAGGCCA AGGAATATGG TCTCGATCAT
    TTGCCCGCCC TCATCTACTT CGAAAACAAG ATCCCGGCCC TCTATGAAGG CGATCTGATG
    AACGAGGATG AGGTGCTCGA GTGGCTTCTT GTCCAGAAAA AGACAGCTAC TATCGAGGAG
    GTTACCGATG AGATCCTGGT CACTCTGATC AACGAACACG AATATGTCGT CGTCTTCTTT
    ACGGGTCCCT GCGAGCCCGG AGAGACCTGT GAGCACACTC TGAACGCCCT GGAAAGCATC
    GACGATGAAT TGGATGAGGC TGGCATAATT TTTGTTACCA CTGAGGATAC CGGAATTGCC
    AAGAAATACA ACGTCAAGAC CTATCCACGT CTGGTGTTCT TCAGGAATCG TGATCCACTT
    CACTTTACCG GTGATCTGGA CGACGAGGAC GAGGTGTTGG CCTGGATTAC TGACGACGAG
    ACCCTTGAAA TTCCCGGAAA AATTGAGGAG GTCAATGTGA AGATGTTGGA CAAGATCTTG
    GCTGAAAACG ATCACGTTGT CGTATTCTTC TACGCTGAGG GCGATAAGAA GGCCCAAAAG
    ATCCTTAACG AGCTGGAGAA CATCGATGAC GAATGCGAGG AAAAAGACAT TGACTTTGTA
    AAGACATCCG ACGACGATAT TGATAAGGAG TACGACCTGC CCGGTCTGCC GGCACTTGCA
    TTTTATAGAC ATAAGTTTAG AACAATTTAC ACCGGTGACC TGATGAAGGA AGAGGAAATT
    CTCGAGTGGG TTATTGATTT GCACGAGTCC ACAGCTGATG TCATTGAATC TGTCGATCGT
    AAGACCCTGC AAGTTTTGAT CAACGATGTT GAGCACCTGG CTGTGTTCTT CTACGACGAT
    GAATGCGAAT CGTGTTCCGA CATCTTGGAG GAGTTGGAGA ACATCGACGA TGACACCGAC
    AAGCACGGAA TACAATTTGT CAAATCAAAT GATGTTAAGC TGGCTCATGA AATTGGCATT
    TTCGCATTTC CAGCTTTGGT CTACTACGAG ACCGGCGTCC CGATTATGTA TGATGGTAAC
    ATTGCAAGCA ATCAGGACGT CTTCAACTGG ATTCTCGAAC AGAAGGCCGA CCAAAGCATT
    CAACTCATTA ATCGTGACCA ACTTTTCGAG TATATAGGCA CCAAAGACTT TTTAGCGGTT
    GTTTTTTACA AAGAAGATGA TCCCGACTCG CCACGAGTGC TGCGGCACAT CGAACTAATC
    GACGACGAGG CTGCGGAATA TGGCATTTAC ATAGTGAAGA TGCACGACAA ACTGATGGCC
    AAGAAGTACG GCTTCCGGAA TCCCCCAGGA CTGACGTATT TCCGAAAGGG CAAGTATATC
    AACTACGACG GCGATATCGA TGACGAGGAG GAGGTACTGG ACTGGCTAAC GAGCCCGGCC
    AACATGGAGA TGACCGATCA CATCGAGCAG GTTAACCGCA AGATGTTCGA GAAGATCCGC
    AAGAACTCCG ACTACGTAGC GGTGATATTC TATAGCGATG AGTGCAAGCA GTGTCCTCGC
    GTCCTGGCCG AGGTGGAACA CATAGACGAC GAAGCGGACA AGGCTGGTAT CGACTTCGTC
    AAAATCGACG ATAAGCAGAT GGCCAAGGAG TACGGAGTGT TCGCCCTGCC TGCCATTGTC
    TTCTTCAAGC CCACATCCAA GGAGCCAGTT ATATACGCCG GTGATCTTTA CGAAGAAGAA
    CAGATCCTAA CTTGGCTGAT CACGCAAAAG GATCCAAGTG GAGATGTTAT CGAAGATCTC
    GAAGGCGAAA GACTTGTTCA TCTGATTGAA GAGTCTGGCT CCATCGCAGT CTACTTTTAT
    GCGGATGGCT GCGAGCAGTG CACCAAGGTT TTGGAGGAGT TGGAAAACAT CGATGATGAT
    TGCGACAAGC ATGGCATAAC ATTCGTTAAG ACCAGGGACT TCTCTGTGGC CGACGGCTAT
    GGCGTGCACG AGTATCCGGC TCTAGTTTAC TTCGAGGGAG GAATCCCCAA CGTATTCGAG
    GGCGAATTGA GCGAGGAGGA AGAGGTTCTT CAGTGGCTTA TCACCCAGAA GACCGAAGAT
    CGTATTGAGC TGATCACTCG CCAGATGCTG GAGACAATGG TGGAGGAGAC GCAGTACCTA
    GCCGTGTATT TCTACAAAAT CAACTGCAAC ATATGCGACC AGATACTAGA GGGCTTGGAA
    CTGATCGATG ACGAATGCGA CGTGTTCGGA ATTCATATGG TCAAGATCCA GGATCCGCAG
    CTGGCAAAAC GTTACTCGAT CAAGACTTTC CCGGCGTTGG TTTATTTCAG AAATGGAAAC
    CCATTACTGT TTGAGGGGGA TCTTCAGAAC GAGCAATCAG TATTGGAATG GCTCATCGAT
    GATGACAACC GCGAGCTGGC TGATGAAATC GAGGAGGTCA ACGAGCGTAT GCTGGATCGC
    CTAATGGCGG AGTCCACTCT ATTGGTTGTT TTCTTTTACG ACGATGATTG TGCTGAGTGC
    GAGGAGATTT TGGAAGAGCT GGAGGAGATC GATGGTGAGG CAGACATGTT CGGCATTGAT
    TTCGTAAAGA TTGCTAGTAT CCAGGCAGCC AAGAAATACG AGATAGTGAA TATACCTTCC
    CTCGTTTATT TCCGAAAACA AGTGCCCGTC CTCTACGACG GCGATCTACA CCAACACGAC
    AAGGTGATTA CCTGGCTAAC ATCACAGGAC GTATTCGAAA TCAAAAACGA AATAGAAGAA
    GTAAACCGAA AAATGCTCGA CAAACTACTC GAGGAAAATG AGTTCTTAGC CGTTTTTTTC
    TACGAACACA ATCAGCCGGA CAGTACTGCA GCGCTGGAAA AACTCGAAAA CATCGACAGC
    GAGACGGATA ACCTGGACAT CACCTTCGTC AAGATGGCCG ACTCTCGTTA TGCCAAGAAA
    TGGGGCGTCA CCAAGCTGCC GGCAATGGTC TACTTCCGCC GTCGGTTTCC CAGCATATAT
    AGGGGTGATC TGTTATCCGA GGACGAAGTG CTGGAGTGGC TACGCAAGAA CCGCTTCCGC
    CAGCCCGAGC TGAACATCTT TATGTACGCT CTGATTGCTC TGGCGGTGGC CTTTGTGGTC
    TACACCGCCT TCCTGCTGCA GTGCTTCAAG CCGGCGCCAC CGCCTCCTGT CCAGCACCCC
    AAGCAGTCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_724492.1
    CDS421..5532
    Translation

    Target ORF information:

    RefSeq Version NM_165485.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_165485.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTGT
    CCAGATCATG AAACATGCCC GCCCAGGGTT ATGGACAAAC AGCAATGCCG CAAGTGCGCC
    AAGGCCTTGC AGGAGCTGGA GAATATCGAC GACGAAGCTG ACCAGCTGGG CATCGGGTTT
    GTGAAGATAC ACGACGAGGC CTTGGCCGAC GAATACAATC TAGGCAATCT GCCAGCCTTG
    GTCTATTACC GCCACCAGAC TCCGATCATA TACGAAGGTG AACTTCAGCG GGAGGAGGAC
    GTCTTGGAAT GGTTGGTGCA GAATAAGTCG ACGGGCGATG AAGATGATGT GATTGAAGAC
    GTCACTTCGA AGACTCTGTC GACGCTGATC AGCAATATCG ACAATCTGGT TGTGCTGTTT
    TATGATCATG GAAACGACGA CTCGATGACC GTGTTAGAGG AGCTAGAGCA AATCGACGAC
    GACTGCGACA AGCATGGCAT TCAGTTTGTG AAAATCGACG ATGCCAAGGC GGCAGCCGAT
    TACGGAATCG ATTCGATTCC GGCCATTGTT TACTTTGAAA AAGAAATTCC AAATGTGTAC
    GACGGCGATC TCATGGACGA GGAGCAGATT CTGAAATGGT TGTTGGGACA GTTGGAACGG
    GATGAGATCG AGGACGTCAC CGACGAAATG CTCGATACAA TGATCAAAGA AGGACGCGTC
    ATTGCCGTGC TGTTCTACGA CAACAACGAC AAGAAGTCCC AGAAAGTGCT CGAGGAGCTG
    GAGAACATTG ACGACGAGTG CGACGCATTG GGCATTACTT TCGTGAAGAT CGACAATCCC
    GAGGAGGCCG TTGAATATGG CATCAATAAA GTTCCTAAAC TGATATACTT TGAAAAAGGC
    ATTCCAACTA TTTACGAGGG CAATCTGGAG GACGAGGAGA AGCTTCTGAA ATGGCTAACA
    GACCAAACGA GTTCCGATCA AATCGAGGAC ATCACCGACG AAATGTTGGA CTTAATCATT
    GAGAAAATGC CCCATGTTGC TGTTCTTTTC TACGACAAAG ACCAAAAGAA ATCACAGAAA
    ATCCTCGCAG AACTGGAAAA CATCGACGAT GAGTGCGATC AGAACGATAT TGCCTTTGTC
    AAGATCGATG ATGACAAGGA GGCCAAAGAA TGGGGTATCG ATGAGATACC ATCGATTGTA
    CTCTTTGAAC GTGGAATTCC ACACATCTAC GAGGGTGATC TGATGAAAGA GGATGAGCTG
    CTTGGCTGGT TGGTGCACCA GAAGCGCTAT TCCGAAATTC CCGAGGTCAC CGATGAGATG
    AAGGACAAGT TGGTCGAGAA CACCGAGCAC TTGGCGGTTA TATTCTACGA CAAGGACGAT
    AAGCAGGATA TGCGCATCCT GAACGAACTG GAGAACATTG ATGACGAGCT GGAGAAGGAG
    GGAATTGTCA TCGTCCGCAT TGATAACGCC GCTGAGGCCA AGGAATATGG TCTCGATCAT
    TTGCCCGCCC TCATCTACTT CGAAAACAAG ATCCCGGCCC TCTATGAAGG CGATCTGATG
    AACGAGGATG AGGTGCTCGA GTGGCTTCTT GTCCAGAAAA AGACAGCTAC TATCGAGGAG
    GTTACCGATG AGATCCTGGT CACTCTGATC AACGAACACG AATATGTCGT CGTCTTCTTT
    ACGGGTCCCT GCGAGCCCGG AGAGACCTGT GAGCACACTC TGAACGCCCT GGAAAGCATC
    GACGATGAAT TGGATGAGGC TGGCATAATT TTTGTTACCA CTGAGGATAC CGGAATTGCC
    AAGAAATACA ACGTCAAGAC CTATCCACGT CTGGTGTTCT TCAGGAATCG TGATCCACTT
    CACTTTACCG GTGATCTGGA CGACGAGGAC GAGGTGTTGG CCTGGATTAC TGACGACGAG
    ACCCTTGAAA TTCCCGGAAA AATTGAGGAG GTCAATGTGA AGATGTTGGA CAAGATCTTG
    GCTGAAAACG ATCACGTTGT CGTATTCTTC TACGCTGAGG GCGATAAGAA GGCCCAAAAG
    ATCCTTAACG AGCTGGAGAA CATCGATGAC GAATGCGAGG AAAAAGACAT TGACTTTGTA
    AAGACATCCG ACGACGATAT TGATAAGGAG TACGACCTGC CCGGTCTGCC GGCACTTGCA
    TTTTATAGAC ATAAGTTTAG AACAATTTAC ACCGGTGACC TGATGAAGGA AGAGGAAATT
    CTCGAGTGGG TTATTGATTT GCACGAGTCC ACAGCTGATG TCATTGAATC TGTCGATCGT
    AAGACCCTGC AAGTTTTGAT CAACGATGTT GAGCACCTGG CTGTGTTCTT CTACGACGAT
    GAATGCGAAT CGTGTTCCGA CATCTTGGAG GAGTTGGAGA ACATCGACGA TGACACCGAC
    AAGCACGGAA TACAATTTGT CAAATCAAAT GATGTTAAGC TGGCTCATGA AATTGGCATT
    TTCGCATTTC CAGCTTTGGT CTACTACGAG ACCGGCGTCC CGATTATGTA TGATGGTAAC
    ATTGCAAGCA ATCAGGACGT CTTCAACTGG ATTCTCGAAC AGAAGGCCGA CCAAAGCATT
    CAACTCATTA ATCGTGACCA ACTTTTCGAG TATATAGGCA CCAAAGACTT TTTAGCGGTT
    GTTTTTTACA AAGAAGATGA TCCCGACTCG CCACGAGTGC TGCGGCACAT CGAACTAATC
    GACGACGAGG CTGCGGAATA TGGCATTTAC ATAGTGAAGA TGCACGACAA ACTGATGGCC
    AAGAAGTACG GCTTCCGGAA TCCCCCAGGA CTGACGTATT TCCGAAAGGG CAAGTATATC
    AACTACGACG GCGATATCGA TGACGAGGAG GAGGTACTGG ACTGGCTAAC GAGCCCGGCC
    AACATGGAGA TGACCGATCA CATCGAGCAG GTTAACCGCA AGATGTTCGA GAAGATCCGC
    AAGAACTCCG ACTACGTAGC GGTGATATTC TATAGCGATG AGTGCAAGCA GTGTCCTCGC
    GTCCTGGCCG AGGTGGAACA CATAGACGAC GAAGCGGACA AGGCTGGTAT CGACTTCGTC
    AAAATCGACG ATAAGCAGAT GGCCAAGGAG TACGGAGTGT TCGCCCTGCC TGCCATTGTC
    TTCTTCAAGC CCACATCCAA GGAGCCAGTT ATATACGCCG GTGATCTTTA CGAAGAAGAA
    CAGATCCTAA CTTGGCTGAT CACGCAAAAG GATCCAAGTG GAGATGTTAT CGAAGATCTC
    GAAGGCGAAA GACTTGTTCA TCTGATTGAA GAGTCTGGCT CCATCGCAGT CTACTTTTAT
    GCGGATGGCT GCGAGCAGTG CACCAAGGTT TTGGAGGAGT TGGAAAACAT CGATGATGAT
    TGCGACAAGC ATGGCATAAC ATTCGTTAAG ACCAGGGACT TCTCTGTGGC CGACGGCTAT
    GGCGTGCACG AGTATCCGGC TCTAGTTTAC TTCGAGGGAG GAATCCCCAA CGTATTCGAG
    GGCGAATTGA GCGAGGAGGA AGAGGTTCTT CAGTGGCTTA TCACCCAGAA GACCGAAGAT
    CGTATTGAGC TGATCACTCG CCAGATGCTG GAGACAATGG TGGAGGAGAC GCAGTACCTA
    GCCGTGTATT TCTACAAAAT CAACTGCAAC ATATGCGACC AGATACTAGA GGGCTTGGAA
    CTGATCGATG ACGAATGCGA CGTGTTCGGA ATTCATATGG TCAAGATCCA GGATCCGCAG
    CTGGCAAAAC GTTACTCGAT CAAGACTTTC CCGGCGTTGG TTTATTTCAG AAATGGAAAC
    CCATTACTGT TTGAGGGGGA TCTTCAGAAC GAGCAATCAG TATTGGAATG GCTCATCGAT
    GATGACAACC GCGAGCTGGC TGATGAAATC GAGGAGGTCA ACGAGCGTAT GCTGGATCGC
    CTAATGGCGG AGTCCACTCT ATTGGTTGTT TTCTTTTACG ACGATGATTG TGCTGAGTGC
    GAGGAGATTT TGGAAGAGCT GGAGGAGATC GATGGTGAGG CAGACATGTT CGGCATTGAT
    TTCGTAAAGA TTGCTAGTAT CCAGGCAGCC AAGAAATACG AGATAGTGAA TATACCTTCC
    CTCGTTTATT TCCGAAAACA AGTGCCCGTC CTCTACGACG GCGATCTACA CCAACACGAC
    AAGGTGATTA CCTGGCTAAC ATCACAGGAC GTATTCGAAA TCAAAAACGA AATAGAAGAA
    GTAAACCGAA AAATGCTCGA CAAACTACTC GAGGAAAATG AGTTCTTAGC CGTTTTTTTC
    TACGAACACA ATCAGCCGGA CAGTACTGCA GCGCTGGAAA AACTCGAAAA CATCGACAGC
    GAGACGGATA ACCTGGACAT CACCTTCGTC AAGATGGCCG ACTCTCGTTA TGCCAAGAAA
    TGGGGCGTCA CCAAGCTGCC GGCAATGGTC TACTTCCGCC GTCGGTTTCC CAGCATATAT
    AGGGGTGATC TGTTATCCGA GGACGAAGTG CTGGAGTGGC TACGCAAGAA CCGCTTCCGC
    CAGCCCGAGC TGAACATCTT TATGTACGCT CTGATTGCTC TGGCGGTGGC CTTTGTGGTC
    TACACCGCCT TCCTGCTGCA GTGCTTCAAG CCGGCGCCAC CGCCTCCTGT CCAGCACCCC
    AAGCAGTCTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa11422
    Clone ID Related Accession (Same CDS sequence) NM_001169593.2
    Accession Version NM_001169593.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169593.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAGCAATGA TGACGGCGAC AGTAATGAAA AAATTGTTAA ATTGCGCTAT
    CCCAAAGAAA GCGATGAGGA TAAAAGAGAA AGATTAAAGC GTCTGCAGAA TTCGATTCGC
    AATGAGAGAT TACATAAAAA AAGACTAGAA ACTGATGATG ATGAAAATGG GGATAACGAA
    GATGAAACAG GGGAGAAAGA TACTGCTAGG GATGACGATG ATGAGGATGA TGATAAAGAT
    AATGAAAATG GGGATGACGA AGATGATAAT GATGAGGACG ACGAGGATGA CGAAGAAGAT
    GATAATAACG GGAACGACGA GGACGACGAG GACGAAGAAG AAGATGACAA TAATGATGAC
    GATGACGAGG ATGATGAAGA TGATAATAAT GGAGACGACG AGGATGACGA AGAGGACAAT
    AATGAAAACG AAGATGAAGA TAATGATGGG GACGACGATG ACGGAGATGA CAATGAGGAC
    GACAAGGACG AGGATAACGA AGAAGATAAT AATAATGACG ACGAAGATGA CGATGAAGAC
    GACGAGGATG ACGAAGAAGA CAATAATGAT AACGACGAGG ATGACGAAGA AGATAATAAT
    AAGGACGACG AAGATAACGA AGAAGATAAT AATGAAGACG ACAAGGACGA TGAGGATGAA
    AACGACGAAG ATGAGGACGA AAATGACAAC GAAGATAATA AGGGAGATGA CGAGGATGAT
    GAGGACGGGG ACGAGGAGGA CGATGACGAT GATGACGGGG ACGACGATAA CAAAAAAGAC
    CATGAGGACG AGGATGATAA CGACGACGGG GACGACAAGG ATAAGGATGA CGAGGACGCA
    ACAGGGGAGG ACGATAATGA GGACGACGAA GAAGAAGAAG AGGATATTAA AAGTAAACCA
    AAATATAGGC ACACGGCCAA AGGTCGAACG ATACACCGTC TTGCTGACCT TTGCTCAGGT
    CGGCTTATAG ATGAAATAGA TGACGAATGT TTCTATGTTG GATTGGGTCA TGACGGCCAT
    TCAGCTAAGC GCGGCAACAA TTTCGTGCCC AACGATTACA AACCATTCCA ATGCTGTCCA
    ACCAAATTGG AGAAGTCAAC GAAAGTTCCT AAGATGACCG CCCAGCGGAT CGGGCATAGC
    GAAGGCGATC AGGGCAAGCG TCCCAGCGGC GGCAACTTCC AGTTTGCCAA CCAGGCGTCC
    AGCAAATCGT CCACCAAGCC AGCTGCCATC AAGAAGCAGG CCAAGCCCTC CAAGGACACC
    GATGACGACG ATGAGGACGA CGAGGACAAG CCCCTGGTCA AGGTTTCCTA TGCCAACAAG
    CGCTCGGGAG GAAGCAACAA GCCGCAGGCT GGCAAGAAGC CGGTAGGCAA GGGCCAGGAC
    AACGACGACC AGTCCCAGGA GGTGGAGAAG GTATCCAAAC AGAAGTCGGC GAAGAAGTCC
    GGCAAACTGA ATGTCAAATC CGGCATTAAT TTTTTCCAAA ATCGACTAAA AAATTTGTAT
    GATATCATTT TTCAGGATAT CTCTCTGTGG GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163064.1
    CDS421..3996
    Translation

    Target ORF information:

    RefSeq Version NM_001169593.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001169593.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAGCAATGA TGACGGCGAC AGTAATGAAA AAATTGTTAA ATTGCGCTAT
    CCCAAAGAAA GCGATGAGGA TAAAAGAGAA AGATTAAAGC GTCTGCAGAA TTCGATTCGC
    AATGAGAGAT TACATAAAAA AAGACTAGAA ACTGATGATG ATGAAAATGG GGATAACGAA
    GATGAAACAG GGGAGAAAGA TACTGCTAGG GATGACGATG ATGAGGATGA TGATAAAGAT
    AATGAAAATG GGGATGACGA AGATGATAAT GATGAGGACG ACGAGGATGA CGAAGAAGAT
    GATAATAACG GGAACGACGA GGACGACGAG GACGAAGAAG AAGATGACAA TAATGATGAC
    GATGACGAGG ATGATGAAGA TGATAATAAT GGAGACGACG AGGATGACGA AGAGGACAAT
    AATGAAAACG AAGATGAAGA TAATGATGGG GACGACGATG ACGGAGATGA CAATGAGGAC
    GACAAGGACG AGGATAACGA AGAAGATAAT AATAATGACG ACGAAGATGA CGATGAAGAC
    GACGAGGATG ACGAAGAAGA CAATAATGAT AACGACGAGG ATGACGAAGA AGATAATAAT
    AAGGACGACG AAGATAACGA AGAAGATAAT AATGAAGACG ACAAGGACGA TGAGGATGAA
    AACGACGAAG ATGAGGACGA AAATGACAAC GAAGATAATA AGGGAGATGA CGAGGATGAT
    GAGGACGGGG ACGAGGAGGA CGATGACGAT GATGACGGGG ACGACGATAA CAAAAAAGAC
    CATGAGGACG AGGATGATAA CGACGACGGG GACGACAAGG ATAAGGATGA CGAGGACGCA
    ACAGGGGAGG ACGATAATGA GGACGACGAA GAAGAAGAAG AGGATATTAA AAGTAAACCA
    AAATATAGGC ACACGGCCAA AGGTCGAACG ATACACCGTC TTGCTGACCT TTGCTCAGGT
    CGGCTTATAG ATGAAATAGA TGACGAATGT TTCTATGTTG GATTGGGTCA TGACGGCCAT
    TCAGCTAAGC GCGGCAACAA TTTCGTGCCC AACGATTACA AACCATTCCA ATGCTGTCCA
    ACCAAATTGG AGAAGTCAAC GAAAGTTCCT AAGATGACCG CCCAGCGGAT CGGGCATAGC
    GAAGGCGATC AGGGCAAGCG TCCCAGCGGC GGCAACTTCC AGTTTGCCAA CCAGGCGTCC
    AGCAAATCGT CCACCAAGCC AGCTGCCATC AAGAAGCAGG CCAAGCCCTC CAAGGACACC
    GATGACGACG ATGAGGACGA CGAGGACAAG CCCCTGGTCA AGGTTTCCTA TGCCAACAAG
    CGCTCGGGAG GAAGCAACAA GCCGCAGGCT GGCAAGAAGC CGGTAGGCAA GGGCCAGGAC
    AACGACGACC AGTCCCAGGA GGTGGAGAAG GTATCCAAAC AGAAGTCGGC GAAGAAGTCC
    GGCAAACTGA ATGTCAAATC CGGCATTAAT TTTTTCCAAA ATCGACTAAA AAATTTGTAT
    GATATCATTT TTCAGGATAT CTCTCTGTGG GAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa11423
    Clone ID Related Accession (Same CDS sequence) NM_001259224.2
    Accession Version NM_001259224.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product lethal (2) 01289, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001259224.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAGCAATGA TGACGGCGAC AGTAATGAAA AAATTGTTAA ATTGCGCTAT
    CCCAAAGAAA GCGATGAGGA TAAAAGAGAA AGATTAAAGC GTCTGCAGAA TTCGATTCGC
    AATGAGAGAT TACATAAAAA AAGACTAGAA ACTGATGATG ATGAAAATGG GGATAACGAA
    GATGAAACAG GGGAGAAAGA TACTGCTAGG GATGACGATG ATGAGGATGA TGATAAAGAT
    AATGAAAATG GGGATGACGA AGATGATAAT GATGAGGACG ACGAGGATGA CGAAGAAGAT
    GATAATAACG GGAACGACGA GGACGACGAG GACGAAGAAG AAGATGACAA TAATGATGAC
    GATGACGAGG ATGATGAAGA TGATAATAAT GGAGACGACG AGGATGACGA AGAGGACAAT
    AATGAAAACG AAGATGAAGA TAATGATGGG GACGACGATG ACGGAGATGA CAATGAGGAC
    GACAAGGACG AGGATAACGA AGAAGATAAT AATAATGACG ACGAAGATGA CGATGAAGAC
    GACGAGGATG ACGAAGAAGA CAATAATGAT AACGACGAGG ATGACGAAGA AGATAATAAT
    AAGGACGACG AAGATAACGA AGAAGATAAT AATGAAGACG ACAAGGACGA TGAGGATGAA
    AACGACGAAG ATGAGGACGA AAATGACAAC GAAGATAATA AGGGAGATGA CGAGGATGAT
    GAGGACGGGG ACGAGGAGGA CGATGACGAT GATGACGGGG ACGACGATAA CAAAAAAGAC
    CATGAGGACG AGGATGATAA CGACGACGGG GACGACAAGG ATAAGGATGA CGAGGACGCA
    ACAGGGGAGG ACGATAATGA GGACGACGAA GAAGAAGAAG AGGATATTAA AAGTAAACCA
    AAATATAGGC ACACGGCCAA AGGTCGAACG ATACACCGTC TTGCTGACCT TTGCTCAGGT
    CGGCTTATAG ATGAAATAGA CTTGGGGAAG AGGAATTCAA ATAGAAATGC ATTTAACCAA
    GATGACGAAT GTTTCTATGT TGGATTGGGT CATGACGGCC ATTCAGCTAA GCGCGGCAAC
    AATTTCGTGC CCAACGATTA CAAACCATTC CAATGCTGTC CAACCAAATT GGAGAAGTCA
    ACGAAAGTTC CTAAGATGAC CGCCCAGCGG ATCGGGCATA GCGAAGGCGA TCAGGGCAAG
    CGTCCCAGCG GCGGCAACTT CCAGTTTGCC AACCAGGCGT CCAGCAAATC GTCCACCAAG
    CCAGCTGCCA TCAAGAAGCA GGCCAAGCCC TCCAAGGACA CCGATGACGA CGATGAGGAC
    GACGAGGACA AGCCCCTGGT CAAGGTTTCC TATGCCAACA AGCGCTCGGG AGGAAGCAAC
    AAGCCGCAGG CTGGCAAGAA GCCGGTAGGC AAGGGCCAGG ACAACGACGA CCAGTCCCAG
    GAGGTGGAGA AGGTATCCAA ACAGAAGTCG GCGAAGAAGT CCGGCAAACT GAATGTCAAA
    TCCGGATATC TCTCTGTGGG AGTAAGGCAA CAGTTTAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246153.1
    CDS421..4002
    Translation

    Target ORF information:

    RefSeq Version NM_001259224.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster lethal (2) 01289 (l(2)01289), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259224.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACTTTCA CCCGCCTCAA GACTCTCTCG CTGCTCGTGT GTGCTCTGCT GGCCCTGAGT 
    TTTCCCGGAC ATGTGAGTGG TGCAGGCAAC AACAACAACA AGAAGGGCTC GCAGCCAGTG
    GCGCCTCCGG AGCCGGAGGC CGTCATCGAG GAGGTCAATG CCAAGCAGCT GGAGAAGCTC
    CTGGCCGACA AGGATTACGT GGCCGTTTTC TGGTATGCGC GAAGCTGCGT GACCTGTGAT
    AAGGTTTTAG CGGAACTCGA GAAAATCGAC GATGACACCG ACTCCTTCGG TGTGGACTTC
    GTGAAAATCA ACGACAAACG ACTAGCCAAA CAGTATGGCA TCAAGAACTT CCCCGCCCTC
    ACCTACTTCA GGGAAAAGGA GCCCATCATA TATGATGGGG ATCTCATGGA CGAGGAAGGA
    GTGCTCGATT TCCTCACCTC CTTGGAGGCC ATGGACTTGC CCGATCGCAT CGAGGAGGTC
    AATGCCAAGA TATTGCAGAA GATCATCGAG GACACCGACT TCGTAGCCGT TCTGTTCTAC
    GACAAAGACC AAAAGAAATC ACAGAAAATC CTCGCAGAAC TGGAAAACAT CGACGATGAG
    TGCGATCAGA ACGATATTGC CTTTGTCAAG ATCGATGATG ACAAGGAGGC CAAAGAATGG
    GGTATCGATG AGATACCATC GATTGTACTC TTTGAACGTG GAATTCCACA CATCTACGAG
    GGTGATCTGA TGAAAGAGGA TGAGCTGCTT GGCTGGTTGG TGCACCAGAA GCGCTATTCC
    GAAATTCCCG AGGTCACCGA TGAGATGAAG GACAAGTTGG TCGAGAACAC CGAGCACTTG
    GCGGTTATAT TCTACGACAA GGACGATAAG CAGGATATGC GCATCCTGAA CGAACTGGAG
    AACATTGATG ACGAGCTGGA GAAGGAGGGA ATTGTCATCG TCCGCATTGA TAACGCCGCT
    GAGGCCAAGG AATATGGTCT CGATCATTTG CCCGCCCTCA TCTACTTCGA AAACAAGATC
    CCGGCCCTCT ATGAAGGCGA TCTGATGAAC GAGGATGAGG TGCTCGAGTG GCTTCTTGTC
    CAGAAAAAGA CAGCTACTAT CGAGGAGGTT ACCGATGAGA TCCTGGTCAC TCTGATCAAC
    GAACACGAAT ATGTCGTCGT CTTCTTTACG GGTCCCTGCG AGCCCGGAGA GACCTGTGAG
    CACACTCTGA ACGCCCTGGA AAGCATCGAC GATGAATTGG ATGAGGCTGG CATAATTTTT
    GTTACCACTG AGGATACCGG AATTGCCAAG AAATACAACG TCAAGACCTA TCCACGTCTG
    GTGTTCTTCA GGAATCGTGA TCCACTTCAC TTTACCGGTG ATCTGGACGA CGAGGACGAG
    GTGTTGGCCT GGATTACTGA CGACGAGACC CTTGAAATTC CCGGAAAAAT TGAGGAGGTC
    AATGTGAAGA TGTTGGACAA GATCTTGGCT GAAAACGATC ACGTTGTCGT ATTCTTCTAC
    GCTGAGGGCG ATAAGAAGGC CCAAAAGATC CTTAACGAGC TGGAGAACAT CGATGACGAA
    TGCGAGGAAA AAGACATTGA CTTTGTAAAG ACATCCGACG ACGATATTGA TAAGGAGTAC
    GACCTGCCCG GTCTGCCGGC ACTTGCATTT TATAGACATA AGTTTAGAAC AATTTACACC
    GGTGACCTGA TGAAGGAAGA GGAAATTCTC GAGTGGGTTA TTGATTTGCA CGAGTCCACA
    GCTGATGTCA TTGAATCTGT CGATCGTAAG ACCCTGCAAG TTTTGATCAA CGATGTTGAG
    CACCTGGCTG TGTTCTTCTA CGACGATGAA TGCGAATCGT GTTCCGACAT CTTGGAGGAG
    TTGGAGAACA TCGACGATGA CACCGACAAG CACGGAATAC AATTTGTCAA ATCAAATGAT
    GTTAAGCTGG CTCATGAAAT TGGCATTTTC GCATTTCCAG CTTTGGTCTA CTACGAGACC
    GGCGTCCCGA TTATGTATGA TGGTAATCTC AAAAATGAAA ACCGTGTGCT GCAGTGGTTA
    GTCAATCAAA AGAGCAATGA TGACGGCGAC AGTAATGAAA AAATTGTTAA ATTGCGCTAT
    CCCAAAGAAA GCGATGAGGA TAAAAGAGAA AGATTAAAGC GTCTGCAGAA TTCGATTCGC
    AATGAGAGAT TACATAAAAA AAGACTAGAA ACTGATGATG ATGAAAATGG GGATAACGAA
    GATGAAACAG GGGAGAAAGA TACTGCTAGG GATGACGATG ATGAGGATGA TGATAAAGAT
    AATGAAAATG GGGATGACGA AGATGATAAT GATGAGGACG ACGAGGATGA CGAAGAAGAT
    GATAATAACG GGAACGACGA GGACGACGAG GACGAAGAAG AAGATGACAA TAATGATGAC
    GATGACGAGG ATGATGAAGA TGATAATAAT GGAGACGACG AGGATGACGA AGAGGACAAT
    AATGAAAACG AAGATGAAGA TAATGATGGG GACGACGATG ACGGAGATGA CAATGAGGAC
    GACAAGGACG AGGATAACGA AGAAGATAAT AATAATGACG ACGAAGATGA CGATGAAGAC
    GACGAGGATG ACGAAGAAGA CAATAATGAT AACGACGAGG ATGACGAAGA AGATAATAAT
    AAGGACGACG AAGATAACGA AGAAGATAAT AATGAAGACG ACAAGGACGA TGAGGATGAA
    AACGACGAAG ATGAGGACGA AAATGACAAC GAAGATAATA AGGGAGATGA CGAGGATGAT
    GAGGACGGGG ACGAGGAGGA CGATGACGAT GATGACGGGG ACGACGATAA CAAAAAAGAC
    CATGAGGACG AGGATGATAA CGACGACGGG GACGACAAGG ATAAGGATGA CGAGGACGCA
    ACAGGGGAGG ACGATAATGA GGACGACGAA GAAGAAGAAG AGGATATTAA AAGTAAACCA
    AAATATAGGC ACACGGCCAA AGGTCGAACG ATACACCGTC TTGCTGACCT TTGCTCAGGT
    CGGCTTATAG ATGAAATAGA CTTGGGGAAG AGGAATTCAA ATAGAAATGC ATTTAACCAA
    GATGACGAAT GTTTCTATGT TGGATTGGGT CATGACGGCC ATTCAGCTAA GCGCGGCAAC
    AATTTCGTGC CCAACGATTA CAAACCATTC CAATGCTGTC CAACCAAATT GGAGAAGTCA
    ACGAAAGTTC CTAAGATGAC CGCCCAGCGG ATCGGGCATA GCGAAGGCGA TCAGGGCAAG
    CGTCCCAGCG GCGGCAACTT CCAGTTTGCC AACCAGGCGT CCAGCAAATC GTCCACCAAG
    CCAGCTGCCA TCAAGAAGCA GGCCAAGCCC TCCAAGGACA CCGATGACGA CGATGAGGAC
    GACGAGGACA AGCCCCTGGT CAAGGTTTCC TATGCCAACA AGCGCTCGGG AGGAAGCAAC
    AAGCCGCAGG CTGGCAAGAA GCCGGTAGGC AAGGGCCAGG ACAACGACGA CCAGTCCCAG
    GAGGTGGAGA AGGTATCCAA ACAGAAGTCG GCGAAGAAGT CCGGCAAACT GAATGTCAAA
    TCCGGATATC TCTCTGTGGG AGTAAGGCAA CAGTTTAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.