east cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	east
Entrez Gene ID	46006
Full Name	enhanced adult sensory threshold
Synonyms	CG4399,Dmel\CG4399,EAST,EG:133E12.4,East,anon-WO03040301.224,l(1)G0014,l(1)G0500,su(w[sp])
General protein information	Preferred Names enhanced adult sensory threshold Names CG4399 gene product from transcript CG4399-RE CG4399-PB CG4399-PC CG4399-PD CG4399-PE CG4399-PF CG4399-PG east-PB east-PC east-PD east-PE east-PF east-PG enhanced adult salt tolerance suppressor of white-spotted
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2B17-2B17\|1-0.16 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201602.2	NP_001188531.1	enhanced adult sensory threshold, isoform D
NM_001258555.1	NP_001245484.1	enhanced adult sensory threshold, isoform E
NM_001258556.2	NP_001245485.1	enhanced adult sensory threshold, isoform F
NM_001201601.2	NP_001188530.1	enhanced adult sensory threshold, isoform C
NM_080125.5	NP_524864.2	enhanced adult sensory threshold, isoform B
NM_001297869.1	NP_001284798.1	enhanced adult sensory threshold, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that the spindle matrix complex, including Chromator/Chriz (Chro), Megator (Mtor), and enhanced adult sensory threshold (East), has a novel nuclear function in reactivation of neural stem cells (NSCs).
Title: An intrinsic mechanism controls reactivation of neural stem cells by spindle matrix proteins.

EAST protein is involved in regulation of the yellow gene in D. melanogaster.
Title: Nuclear matrix protein EAST is involved in regulation of transcription of the yellow gene in Drosophila melanogaster.

Chro activates and east inhibits processes and genes involved in tissue destruction and remodeling.
Title: EAST and Chromator control the destruction and remodeling of muscles during Drosophila metamorphosis.

propose that EAST acts as an ion sensor that modulates gene expression in response to changing intracellular ion concentrations
Title: The extrachromosomal East protein of Drosophila can associate with polytene chromosomes and regulate gene expression.

Megator and EAST interact to form a nuclear endoskeleton and as well are important components of the putative spindle matrix complex during mitosis
Title: EAST interacts with Megator and localizes to the putative spindle matrix during mitosis in Drosophila.

The following east gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the east cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02400	NM_001201602.2 Latest version!	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02400	NM_001201601.2 Latest version!	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02400	NM_080125.5 Latest version!	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa02753	NM_001258555.1 Latest version!	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa02753	NM_001258556.2 Latest version!	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa22257	NM_001297869.1 Latest version!	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

east ( NM_001201602.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188531.1 Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa02400
Clone ID Related Accession (Same CDS sequence)	NM_001201602.2 , NM_001201601.2 , NM_080125.5
Accession Version	NM_001201602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhanced adult sensory threshold, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001201602.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCAACGAGG AGAAGCTCAC CGTGGATAAC TGCTTGTGCG ACGCATGTTT TCGACACGTG 
GACCGCCGGG CAAATGTGCC ATCCTATAAG AAGCGTCTTT CCGCTTCAGG TCACTTGGAG 
ATGGGGTCTG CAGCGGGATC TGCACTAGAG AAACAGTTTG CTGGCGACAG CGGCGTCATT 
ACGGAATCGG GTGGCGAAGC TGGTTCTACG GCAGCTGTGG CCGTGCAGCA ACGATCTTGT 
GGCGTGAAGG ACTGCGTCGA AGCGGCACGA CACTCGCTGC GGCGCAAGTG CATACGCAAG 
AGAGTAAAGA AGTATCAGCT CAGCCTGGAG ATTCCCGCAG GCTCGTCGAA CGTGGGGCTG 
TGTGAGGCAC ATTACAATAC GGTCATCCAA TTTTCCGGCT GCGTTCTTTG CAAGCGTAGA 
TTAGGCAAGA ACCATATGTA CAACATAACC ACGCAGGACA CAATTCGACT GGAAAAGGCG 
CTGTCCGAGA TGGGCATCCC AGTTCAGCTT GGCATGGGCA CTGCAGTCTG CAAGCTGTGT 
CGCTATTTTG CCAACCTTTT GATAAAGCCA CCGGATAGCA CCAAGGCACA AAAGGCGGAA 
TTCGTGAAGA ACTACAGAAA GAGGCTCCTC AAGGTGCATA ATCTGCAGGA TGGCAGTCAT 
GAGCTGTCCG AAGCGGATGA AGAAGAGGCA CCTAATGCAA CGGAGACAGA AAGGCCAACC 
TCAGACGGAC ACGAAGATCC CGAGATGCCC ATGGTAGCGG ACTATGATGG ACCTACCGAC 
TCCAATTCCA GTAGTTCTTC GACTGCAGCC CTGGACACCA GCAAACAAAT GTCCAAGCTT 
CAGGCCATCC TGCAGCAAAA TGTGGGAGCG GATGCGGCAG GAGCTGCGGG AACAGGAACT 
GTTGCAGCAA GTCCCGGAGG AAGCGGATCT GGGGCAGATA TCTCTAACGT ATTGCGAGGG 
AATCCGAACA TTTCCATGCG CGAACTTTTC CACGGCGAGG AAGAGCTGGG TGTGCAGTTC 
AAGGTGCCGT TCGGATGCAG CAGCAGCCAG CGTACTCCGG AGGGCTGGAC ACGAGTGCAG 
ACTTTCCTAC AATACGATGA GCCGACGCGC CGCCTCTGGG AGGAGTTGCA AAAGCCGTAC 
GGAAATCAGA GCTCATTTCT GCGCCACTTG ATACTATTAG AGAAGTACTA CCGAAACGGA 
GATCTCGTCC TAGCACCGCA TGCTTCCTCC AATGCCACGG TTTACACAGA GACTGTTCGT 
CAGCGGCTGA ATTCGTTTGA TCACGGTCAC TGCGGTGGAT TGAACATCGC AGGCAGCCCT 
TCTTCTTCGG GTTCCGGCAA GCGCAGTGGA GTTCCTCAAC CTACGGGTGC CAGTGTGCTG 
GCCACCGCCC TCACAACACC CTTGACAAGC CATTCATCCT CCTCTGCATC CATTTCCTCC 
GAACAGCATT CGTCGGTTGA TCCTGTCATT CCGCTGGTAG ACCTCAATGA TGACGATGAA 
GGCGAAGATG GGGCAGGAGG AGCGGGCGAA AGGGAGTCGA CAAATAGGCA GCAGGACGTA 
ATCTTGGAAT GCCTTAGAAC TGCCTCTGTG GACAAGCTGA CTAAGCAGCT CAGCTCGAAT 
GCGGTGACGA TTATCGCCCG GCCCAAAGAC AAATCGCAGC TCTCCTGCAA CAGCGGATCC 
TCCACGTCCA TTTCCAGCTC CTCGTCCGCT ATTTCCTCGC CGGAGGAAGT GGCCGTCACT 
AAGGTTACAG CAGTCGCACC AGTCCAGTCC AAGGATGCAC CGCCACTGGC GCCAGCAAGT 
AGCGGTGTTA GCAACAGTCG TAGTATCCTT AAAACCAACC TCTTGGGCAT GAACAAGGCC 
GTGGAACTCG TGCCCTTAAC GACTGCCCCC CACGCTTACA AGCCAACTGG ATGCCATAAG 
CCTGAGAAAC AGCAAAAGAT TCTTGACGTG GCCAATAAGC AGCCCGGTAG CCAGGGGGAA 
CCGGTACCAT CAAGCGCCTT GCTTGGCCTG CAGTCAAAGC TAAAGCCTCC AACGCATCAG 
CAGCAGGTCA GCGGATCAGG AGCGGGAACT AGTGGTTCTC AGAAGCCATC TAATGTGGCG 
CAATTGCTTA GCTCTCCACC GGAGCTAATC AGCTTGCATC GACGGCAGAC CAGCGGAGCA 
GCAGCGGGGT CCAGCAGCTT CCTTCAGGGC AAGAGGCTTC AACTTCCACG ATCTGGAGCA 
GGGCCTTCAG GAGCGGGAAC GGGAACAGGC GCTGGAGCAG CAGGAAGCCG CAGTGCGGGT 
GGACCACCAC CGCCCAATGT GGTCATACTG CCGGACGCCT TAACCCCCCA GGAGCGACAC 
GAGAGCAAGA GCTGGAAGCC AACGCTGATA CCGCTGGAGG ATCAGCACAA GGTGCCGAAC 
AAATCACATG CTCTTTATCA GACCGCCGAC GGTCGAAGGT TGCCCGCCCT GGTGCAAGTG 
CAGTCTGGTG GCAAGCCATA CCTCATCTCT ATCTTCGACT ATAACCGCAT GTGCATCTTG 
CGAAGGGAAA AGCTGATGCG GGACCAGTTG CTCAAGAGTA ACGCCAAGCC AAAGCCGCAG 
AACCAGCAAC AGCAGCAGGG CCAAACGCAC CAGCAGCAGC AGAATTCCGC CGCATCGGCG 
GCTGCCTTCT CCAACATGGT GAAGTTGGCC CAGCAACACA CGGCGCGACA GCAGCTTCAG 
CAGCTGCAAC AGAAGCCACA ACAGCAGCAA CAATTGCCCA CTTTGCAGCC AGGTGGGGTG 
CGACTTGCCC GGCTGCCGCA AAAACTACTG ATGCCACCAC TGACTAATCC GCAGATTGGC 
AGTCAAGCAC CCAACTTACA GCCGTTGCTG TCTAGTACGC TGGATAACAG CAACAACTGC 
TGGCTGTGGA AAAACTTTCC TGATCCCAAT CAGTATCTGC TAAATGGAAA CGGAGGGGGT 
GCCGGGAGCT CCTCCAGCAA GTTGCCACAT CTCACGGCCA AACCAGCCAC GGCAACTAGT 
AGCTCCGGAG CGGCCAACAA ATCAGCAGGA AGCCTATTTA CCCTCAAGCA GCAGCAGCAC 
CAGCAGAAAC TCATCGACAA CGCTATCATG TCAAAGATAC CCAAAAGTCT GACAGTAATA 
CCGCAGCAGA TGGGTGGTAA TACCGGTGGC GATATGGGGG GCAGCAGCTC CTCCGGCAAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188531.1
CDS	267..7172
Translation	MSSRKVPGGSGGADESTAAAAPLDDNANASVEIPDSSEEPAMGVGEEMSIISKTRTSTLSVEPAKEPTVTAELEGEKELESNPVSKTPRSTPTPTLTPAVTPTASDGVAAKSVRVTRHSSPLLLIISPTTSRREVGDGELDTEEPTGSGGQRKSSVERSLAPVIRGRKSIKDLKEAKEVKSEEPPAAASESRAASGVTPGQVKEQHVADGNEMESLPITDKKDHKDTKDKGDERETDQEEEKEKSADTEIIADTEKTSEKQKYTEKDKAADKDGGKEKDIDANKDIDKEKEKVKEVLPPVVPIAPVTPTCNRVTRKSHAQEQAINTRVTRNRRQSSTVGANSTASLVAASSSVTEQPPPSRGRRKKPVVVAPPLEPAVKRKRSQDVEADSDANNSTKYSKVEVVKSEEAEAPEEDSSAVPIKQESVDGNEVSSISPTVTPTPTPAPTPAPVPGSRRGRGRPQNRNSSSPATTTRATRLSKAGSPVILTPVAQEPAPPKRRRVGSSTRKTVSASSLAPSSQGGAGDEDSKDSMASSMDDLLMAAADIKQEKLTPDFDDSLMPEGLPSTSGASSANGHSCTEPLTVDTEINVKPADSKVKPKESPVVAVEESPSQSETQSAKVSAHAGKAPSLSPDMISEGVSAVSVRKFYKKPEFLENNLGIEKDPELGEIVQTVSNNDTETDVEMAVDGEVNQPSTPKSQDKKKEEQEKNQKSGLKAAKKAPAKLEPKAEDISEILTDVPVDISTEAVEIIEEAEEDTCSNSSIKPGELRLDESNDEPELLLEDALIVNGDENETPDQPEEKEDQVEFFHTGEYDDFEHEIMVELAKEGVLDASGNALSQQKVELEHPEDVTLHESKNDIEAEESVERKPLKDPSVADEMEDMNEESYIDIKDQTNQLLVEHLAEEAMEADCGPEDNKENLSTSASSTAADGLDIQLAIKEDDDEEKPLAVIADEQKPGLLLTNDMKVDEKPNGKQESVCDEHVQLVPNLRQEQEIHLQNLGLLTHQAAEHRRKCLLEAQARQAQMQLQQHHHHQHKRQGARGGGSATHVESSGTLKTVIKLNRSSNGGVSGSGGLPTGTVIHGGCGSSSASSTSSSSVGSATRKSSGTLGSGAGAGAGVRRQSLKMTFQKGRARGHGAADRSADQYGAHAEDSYYTIQNENEDSSHSESQGQTDHGFYQMVKKDEKEKILIPEKASSFKFHPGRLCEDQCYYCSGKFGLYDTPCHVGQIKSVERQQKILANEEKLTVDNCLCDACFRHVDRRANVPSYKKRLSASGHLEMGSAAGSALEKQFAGDSGVITESGGEAGSTAAVAVQQRSCGVKDCVEAARHSLRRKCIRKRVKKYQLSLEIPAGSSNVGLCEAHYNTVIQFSGCVLCKRRLGKNHMYNITTQDTIRLEKALSEMGIPVQLGMGTAVCKLCRYFANLLIKPPDSTKAQKAEFVKNYRKRLLKVHNLQDGSHELSEADEEEAPNATETERPTSDGHEDPEMPMVADYDGPTDSNSSSSSTAALDTSKQMSKLQAILQQNVGADAAGAAGTGTVAASPGGSGSGADISNVLRGNPNISMRELFHGEEELGVQFKVPFGCSSSQRTPEGWTRVQTFLQYDEPTRRLWEELQKPYGNQSSFLRHLILLEKYYRNGDLVLAPHASSNATVYTETVRQRLNSFDHGHCGGLNIAGSPSSSGSGKRSGVPQPTGASVLATALTTPLTSHSSSSASISSEQHSSVDPVIPLVDLNDDDEGEDGAGGAGERESTNRQQDVILECLRTASVDKLTKQLSSNAVTIIARPKDKSQLSCNSGSSTSISSSSSAISSPEEVAVTKVTAVAPVQSKDAPPLAPASSGVSNSRSILKTNLLGMNKAVELVPLTTAPHAYKPTGCHKPEKQQKILDVANKQPGSQGEPVPSSALLGLQSKLKPPTHQQQVSGSGAGTSGSQKPSNVAQLLSSPPELISLHRRQTSGAAAGSSSFLQGKRLQLPRSGAGPSGAGTGTGAGAAGSRSAGGPPPPNVVILPDALTPQERHESKSWKPTLIPLEDQHKVPNKSHALYQTADGRRLPALVQVQSGGKPYLISIFDYNRMCILRREKLMRDQLLKSNAKPKPQNQQQQQGQTHQQQQNSAASAAAFSNMVKLAQQHTARQQLQQLQQKPQQQQQLPTLQPGGVRLARLPQKLLMPPLTNPQIGSQAPNLQPLLSSTLDNSNNCWLWKNFPDPNQYLLNGNGGGAGSSSSKLPHLTAKPATATSSSGAANKSAGSLFTLKQQQHQQKLIDNAIMSKIPKSLTVIPQQMGGNTGGDMGGSSSSGKD

Target ORF information:

RefSeq Version	NM_001201602.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201602.2

ORF Insert Sequence:

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCAACGAGG AGAAGCTCAC CGTGGATAAC TGCTTGTGCG ACGCATGTTT TCGACACGTG 
GACCGCCGGG CAAATGTGCC ATCCTATAAG AAGCGTCTTT CCGCTTCAGG TCACTTGGAG 
ATGGGGTCTG CAGCGGGATC TGCACTAGAG AAACAGTTTG CTGGCGACAG CGGCGTCATT 
ACGGAATCGG GTGGCGAAGC TGGTTCTACG GCAGCTGTGG CCGTGCAGCA ACGATCTTGT 
GGCGTGAAGG ACTGCGTCGA AGCGGCACGA CACTCGCTGC GGCGCAAGTG CATACGCAAG 
AGAGTAAAGA AGTATCAGCT CAGCCTGGAG ATTCCCGCAG GCTCGTCGAA CGTGGGGCTG 
TGTGAGGCAC ATTACAATAC GGTCATCCAA TTTTCCGGCT GCGTTCTTTG CAAGCGTAGA 
TTAGGCAAGA ACCATATGTA CAACATAACC ACGCAGGACA CAATTCGACT GGAAAAGGCG 
CTGTCCGAGA TGGGCATCCC AGTTCAGCTT GGCATGGGCA CTGCAGTCTG CAAGCTGTGT 
CGCTATTTTG CCAACCTTTT GATAAAGCCA CCGGATAGCA CCAAGGCACA AAAGGCGGAA 
TTCGTGAAGA ACTACAGAAA GAGGCTCCTC AAGGTGCATA ATCTGCAGGA TGGCAGTCAT 
GAGCTGTCCG AAGCGGATGA AGAAGAGGCA CCTAATGCAA CGGAGACAGA AAGGCCAACC 
TCAGACGGAC ACGAAGATCC CGAGATGCCC ATGGTAGCGG ACTATGATGG ACCTACCGAC 
TCCAATTCCA GTAGTTCTTC GACTGCAGCC CTGGACACCA GCAAACAAAT GTCCAAGCTT 
CAGGCCATCC TGCAGCAAAA TGTGGGAGCG GATGCGGCAG GAGCTGCGGG AACAGGAACT 
GTTGCAGCAA GTCCCGGAGG AAGCGGATCT GGGGCAGATA TCTCTAACGT ATTGCGAGGG 
AATCCGAACA TTTCCATGCG CGAACTTTTC CACGGCGAGG AAGAGCTGGG TGTGCAGTTC 
AAGGTGCCGT TCGGATGCAG CAGCAGCCAG CGTACTCCGG AGGGCTGGAC ACGAGTGCAG 
ACTTTCCTAC AATACGATGA GCCGACGCGC CGCCTCTGGG AGGAGTTGCA AAAGCCGTAC 
GGAAATCAGA GCTCATTTCT GCGCCACTTG ATACTATTAG AGAAGTACTA CCGAAACGGA 
GATCTCGTCC TAGCACCGCA TGCTTCCTCC AATGCCACGG TTTACACAGA GACTGTTCGT 
CAGCGGCTGA ATTCGTTTGA TCACGGTCAC TGCGGTGGAT TGAACATCGC AGGCAGCCCT 
TCTTCTTCGG GTTCCGGCAA GCGCAGTGGA GTTCCTCAAC CTACGGGTGC CAGTGTGCTG 
GCCACCGCCC TCACAACACC CTTGACAAGC CATTCATCCT CCTCTGCATC CATTTCCTCC 
GAACAGCATT CGTCGGTTGA TCCTGTCATT CCGCTGGTAG ACCTCAATGA TGACGATGAA 
GGCGAAGATG GGGCAGGAGG AGCGGGCGAA AGGGAGTCGA CAAATAGGCA GCAGGACGTA 
ATCTTGGAAT GCCTTAGAAC TGCCTCTGTG GACAAGCTGA CTAAGCAGCT CAGCTCGAAT 
GCGGTGACGA TTATCGCCCG GCCCAAAGAC AAATCGCAGC TCTCCTGCAA CAGCGGATCC 
TCCACGTCCA TTTCCAGCTC CTCGTCCGCT ATTTCCTCGC CGGAGGAAGT GGCCGTCACT 
AAGGTTACAG CAGTCGCACC AGTCCAGTCC AAGGATGCAC CGCCACTGGC GCCAGCAAGT 
AGCGGTGTTA GCAACAGTCG TAGTATCCTT AAAACCAACC TCTTGGGCAT GAACAAGGCC 
GTGGAACTCG TGCCCTTAAC GACTGCCCCC CACGCTTACA AGCCAACTGG ATGCCATAAG 
CCTGAGAAAC AGCAAAAGAT TCTTGACGTG GCCAATAAGC AGCCCGGTAG CCAGGGGGAA 
CCGGTACCAT CAAGCGCCTT GCTTGGCCTG CAGTCAAAGC TAAAGCCTCC AACGCATCAG 
CAGCAGGTCA GCGGATCAGG AGCGGGAACT AGTGGTTCTC AGAAGCCATC TAATGTGGCG 
CAATTGCTTA GCTCTCCACC GGAGCTAATC AGCTTGCATC GACGGCAGAC CAGCGGAGCA 
GCAGCGGGGT CCAGCAGCTT CCTTCAGGGC AAGAGGCTTC AACTTCCACG ATCTGGAGCA 
GGGCCTTCAG GAGCGGGAAC GGGAACAGGC GCTGGAGCAG CAGGAAGCCG CAGTGCGGGT 
GGACCACCAC CGCCCAATGT GGTCATACTG CCGGACGCCT TAACCCCCCA GGAGCGACAC 
GAGAGCAAGA GCTGGAAGCC AACGCTGATA CCGCTGGAGG ATCAGCACAA GGTGCCGAAC 
AAATCACATG CTCTTTATCA GACCGCCGAC GGTCGAAGGT TGCCCGCCCT GGTGCAAGTG 
CAGTCTGGTG GCAAGCCATA CCTCATCTCT ATCTTCGACT ATAACCGCAT GTGCATCTTG 
CGAAGGGAAA AGCTGATGCG GGACCAGTTG CTCAAGAGTA ACGCCAAGCC AAAGCCGCAG 
AACCAGCAAC AGCAGCAGGG CCAAACGCAC CAGCAGCAGC AGAATTCCGC CGCATCGGCG 
GCTGCCTTCT CCAACATGGT GAAGTTGGCC CAGCAACACA CGGCGCGACA GCAGCTTCAG 
CAGCTGCAAC AGAAGCCACA ACAGCAGCAA CAATTGCCCA CTTTGCAGCC AGGTGGGGTG 
CGACTTGCCC GGCTGCCGCA AAAACTACTG ATGCCACCAC TGACTAATCC GCAGATTGGC 
AGTCAAGCAC CCAACTTACA GCCGTTGCTG TCTAGTACGC TGGATAACAG CAACAACTGC 
TGGCTGTGGA AAAACTTTCC TGATCCCAAT CAGTATCTGC TAAATGGAAA CGGAGGGGGT 
GCCGGGAGCT CCTCCAGCAA GTTGCCACAT CTCACGGCCA AACCAGCCAC GGCAACTAGT 
AGCTCCGGAG CGGCCAACAA ATCAGCAGGA AGCCTATTTA CCCTCAAGCA GCAGCAGCAC 
CAGCAGAAAC TCATCGACAA CGCTATCATG TCAAAGATAC CCAAAAGTCT GACAGTAATA 
CCGCAGCAGA TGGGTGGTAA TACCGGTGGC GATATGGGGG GCAGCAGCTC CTCCGGCAAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

east ( NM_001201601.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188530.1 Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant C, mRNA.

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CloneID	OFa02400
Clone ID Related Accession (Same CDS sequence)	NM_001201602.2 , NM_001201601.2 , NM_080125.5
Accession Version	NM_001201601.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhanced adult sensory threshold, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001201601.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCAACGAGG AGAAGCTCAC CGTGGATAAC TGCTTGTGCG ACGCATGTTT TCGACACGTG 
GACCGCCGGG CAAATGTGCC ATCCTATAAG AAGCGTCTTT CCGCTTCAGG TCACTTGGAG 
ATGGGGTCTG CAGCGGGATC TGCACTAGAG AAACAGTTTG CTGGCGACAG CGGCGTCATT 
ACGGAATCGG GTGGCGAAGC TGGTTCTACG GCAGCTGTGG CCGTGCAGCA ACGATCTTGT 
GGCGTGAAGG ACTGCGTCGA AGCGGCACGA CACTCGCTGC GGCGCAAGTG CATACGCAAG 
AGAGTAAAGA AGTATCAGCT CAGCCTGGAG ATTCCCGCAG GCTCGTCGAA CGTGGGGCTG 
TGTGAGGCAC ATTACAATAC GGTCATCCAA TTTTCCGGCT GCGTTCTTTG CAAGCGTAGA 
TTAGGCAAGA ACCATATGTA CAACATAACC ACGCAGGACA CAATTCGACT GGAAAAGGCG 
CTGTCCGAGA TGGGCATCCC AGTTCAGCTT GGCATGGGCA CTGCAGTCTG CAAGCTGTGT 
CGCTATTTTG CCAACCTTTT GATAAAGCCA CCGGATAGCA CCAAGGCACA AAAGGCGGAA 
TTCGTGAAGA ACTACAGAAA GAGGCTCCTC AAGGTGCATA ATCTGCAGGA TGGCAGTCAT 
GAGCTGTCCG AAGCGGATGA AGAAGAGGCA CCTAATGCAA CGGAGACAGA AAGGCCAACC 
TCAGACGGAC ACGAAGATCC CGAGATGCCC ATGGTAGCGG ACTATGATGG ACCTACCGAC 
TCCAATTCCA GTAGTTCTTC GACTGCAGCC CTGGACACCA GCAAACAAAT GTCCAAGCTT 
CAGGCCATCC TGCAGCAAAA TGTGGGAGCG GATGCGGCAG GAGCTGCGGG AACAGGAACT 
GTTGCAGCAA GTCCCGGAGG AAGCGGATCT GGGGCAGATA TCTCTAACGT ATTGCGAGGG 
AATCCGAACA TTTCCATGCG CGAACTTTTC CACGGCGAGG AAGAGCTGGG TGTGCAGTTC 
AAGGTGCCGT TCGGATGCAG CAGCAGCCAG CGTACTCCGG AGGGCTGGAC ACGAGTGCAG 
ACTTTCCTAC AATACGATGA GCCGACGCGC CGCCTCTGGG AGGAGTTGCA AAAGCCGTAC 
GGAAATCAGA GCTCATTTCT GCGCCACTTG ATACTATTAG AGAAGTACTA CCGAAACGGA 
GATCTCGTCC TAGCACCGCA TGCTTCCTCC AATGCCACGG TTTACACAGA GACTGTTCGT 
CAGCGGCTGA ATTCGTTTGA TCACGGTCAC TGCGGTGGAT TGAACATCGC AGGCAGCCCT 
TCTTCTTCGG GTTCCGGCAA GCGCAGTGGA GTTCCTCAAC CTACGGGTGC CAGTGTGCTG 
GCCACCGCCC TCACAACACC CTTGACAAGC CATTCATCCT CCTCTGCATC CATTTCCTCC 
GAACAGCATT CGTCGGTTGA TCCTGTCATT CCGCTGGTAG ACCTCAATGA TGACGATGAA 
GGCGAAGATG GGGCAGGAGG AGCGGGCGAA AGGGAGTCGA CAAATAGGCA GCAGGACGTA 
ATCTTGGAAT GCCTTAGAAC TGCCTCTGTG GACAAGCTGA CTAAGCAGCT CAGCTCGAAT 
GCGGTGACGA TTATCGCCCG GCCCAAAGAC AAATCGCAGC TCTCCTGCAA CAGCGGATCC 
TCCACGTCCA TTTCCAGCTC CTCGTCCGCT ATTTCCTCGC CGGAGGAAGT GGCCGTCACT 
AAGGTTACAG CAGTCGCACC AGTCCAGTCC AAGGATGCAC CGCCACTGGC GCCAGCAAGT 
AGCGGTGTTA GCAACAGTCG TAGTATCCTT AAAACCAACC TCTTGGGCAT GAACAAGGCC 
GTGGAACTCG TGCCCTTAAC GACTGCCCCC CACGCTTACA AGCCAACTGG ATGCCATAAG 
CCTGAGAAAC AGCAAAAGAT TCTTGACGTG GCCAATAAGC AGCCCGGTAG CCAGGGGGAA 
CCGGTACCAT CAAGCGCCTT GCTTGGCCTG CAGTCAAAGC TAAAGCCTCC AACGCATCAG 
CAGCAGGTCA GCGGATCAGG AGCGGGAACT AGTGGTTCTC AGAAGCCATC TAATGTGGCG 
CAATTGCTTA GCTCTCCACC GGAGCTAATC AGCTTGCATC GACGGCAGAC CAGCGGAGCA 
GCAGCGGGGT CCAGCAGCTT CCTTCAGGGC AAGAGGCTTC AACTTCCACG ATCTGGAGCA 
GGGCCTTCAG GAGCGGGAAC GGGAACAGGC GCTGGAGCAG CAGGAAGCCG CAGTGCGGGT 
GGACCACCAC CGCCCAATGT GGTCATACTG CCGGACGCCT TAACCCCCCA GGAGCGACAC 
GAGAGCAAGA GCTGGAAGCC AACGCTGATA CCGCTGGAGG ATCAGCACAA GGTGCCGAAC 
AAATCACATG CTCTTTATCA GACCGCCGAC GGTCGAAGGT TGCCCGCCCT GGTGCAAGTG 
CAGTCTGGTG GCAAGCCATA CCTCATCTCT ATCTTCGACT ATAACCGCAT GTGCATCTTG 
CGAAGGGAAA AGCTGATGCG GGACCAGTTG CTCAAGAGTA ACGCCAAGCC AAAGCCGCAG 
AACCAGCAAC AGCAGCAGGG CCAAACGCAC CAGCAGCAGC AGAATTCCGC CGCATCGGCG 
GCTGCCTTCT CCAACATGGT GAAGTTGGCC CAGCAACACA CGGCGCGACA GCAGCTTCAG 
CAGCTGCAAC AGAAGCCACA ACAGCAGCAA CAATTGCCCA CTTTGCAGCC AGGTGGGGTG 
CGACTTGCCC GGCTGCCGCA AAAACTACTG ATGCCACCAC TGACTAATCC GCAGATTGGC 
AGTCAAGCAC CCAACTTACA GCCGTTGCTG TCTAGTACGC TGGATAACAG CAACAACTGC 
TGGCTGTGGA AAAACTTTCC TGATCCCAAT CAGTATCTGC TAAATGGAAA CGGAGGGGGT 
GCCGGGAGCT CCTCCAGCAA GTTGCCACAT CTCACGGCCA AACCAGCCAC GGCAACTAGT 
AGCTCCGGAG CGGCCAACAA ATCAGCAGGA AGCCTATTTA CCCTCAAGCA GCAGCAGCAC 
CAGCAGAAAC TCATCGACAA CGCTATCATG TCAAAGATAC CCAAAAGTCT GACAGTAATA 
CCGCAGCAGA TGGGTGGTAA TACCGGTGGC GATATGGGGG GCAGCAGCTC CTCCGGCAAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188530.1
CDS	209..7114
Translation	MSSRKVPGGSGGADESTAAAAPLDDNANASVEIPDSSEEPAMGVGEEMSIISKTRTSTLSVEPAKEPTVTAELEGEKELESNPVSKTPRSTPTPTLTPAVTPTASDGVAAKSVRVTRHSSPLLLIISPTTSRREVGDGELDTEEPTGSGGQRKSSVERSLAPVIRGRKSIKDLKEAKEVKSEEPPAAASESRAASGVTPGQVKEQHVADGNEMESLPITDKKDHKDTKDKGDERETDQEEEKEKSADTEIIADTEKTSEKQKYTEKDKAADKDGGKEKDIDANKDIDKEKEKVKEVLPPVVPIAPVTPTCNRVTRKSHAQEQAINTRVTRNRRQSSTVGANSTASLVAASSSVTEQPPPSRGRRKKPVVVAPPLEPAVKRKRSQDVEADSDANNSTKYSKVEVVKSEEAEAPEEDSSAVPIKQESVDGNEVSSISPTVTPTPTPAPTPAPVPGSRRGRGRPQNRNSSSPATTTRATRLSKAGSPVILTPVAQEPAPPKRRRVGSSTRKTVSASSLAPSSQGGAGDEDSKDSMASSMDDLLMAAADIKQEKLTPDFDDSLMPEGLPSTSGASSANGHSCTEPLTVDTEINVKPADSKVKPKESPVVAVEESPSQSETQSAKVSAHAGKAPSLSPDMISEGVSAVSVRKFYKKPEFLENNLGIEKDPELGEIVQTVSNNDTETDVEMAVDGEVNQPSTPKSQDKKKEEQEKNQKSGLKAAKKAPAKLEPKAEDISEILTDVPVDISTEAVEIIEEAEEDTCSNSSIKPGELRLDESNDEPELLLEDALIVNGDENETPDQPEEKEDQVEFFHTGEYDDFEHEIMVELAKEGVLDASGNALSQQKVELEHPEDVTLHESKNDIEAEESVERKPLKDPSVADEMEDMNEESYIDIKDQTNQLLVEHLAEEAMEADCGPEDNKENLSTSASSTAADGLDIQLAIKEDDDEEKPLAVIADEQKPGLLLTNDMKVDEKPNGKQESVCDEHVQLVPNLRQEQEIHLQNLGLLTHQAAEHRRKCLLEAQARQAQMQLQQHHHHQHKRQGARGGGSATHVESSGTLKTVIKLNRSSNGGVSGSGGLPTGTVIHGGCGSSSASSTSSSSVGSATRKSSGTLGSGAGAGAGVRRQSLKMTFQKGRARGHGAADRSADQYGAHAEDSYYTIQNENEDSSHSESQGQTDHGFYQMVKKDEKEKILIPEKASSFKFHPGRLCEDQCYYCSGKFGLYDTPCHVGQIKSVERQQKILANEEKLTVDNCLCDACFRHVDRRANVPSYKKRLSASGHLEMGSAAGSALEKQFAGDSGVITESGGEAGSTAAVAVQQRSCGVKDCVEAARHSLRRKCIRKRVKKYQLSLEIPAGSSNVGLCEAHYNTVIQFSGCVLCKRRLGKNHMYNITTQDTIRLEKALSEMGIPVQLGMGTAVCKLCRYFANLLIKPPDSTKAQKAEFVKNYRKRLLKVHNLQDGSHELSEADEEEAPNATETERPTSDGHEDPEMPMVADYDGPTDSNSSSSSTAALDTSKQMSKLQAILQQNVGADAAGAAGTGTVAASPGGSGSGADISNVLRGNPNISMRELFHGEEELGVQFKVPFGCSSSQRTPEGWTRVQTFLQYDEPTRRLWEELQKPYGNQSSFLRHLILLEKYYRNGDLVLAPHASSNATVYTETVRQRLNSFDHGHCGGLNIAGSPSSSGSGKRSGVPQPTGASVLATALTTPLTSHSSSSASISSEQHSSVDPVIPLVDLNDDDEGEDGAGGAGERESTNRQQDVILECLRTASVDKLTKQLSSNAVTIIARPKDKSQLSCNSGSSTSISSSSSAISSPEEVAVTKVTAVAPVQSKDAPPLAPASSGVSNSRSILKTNLLGMNKAVELVPLTTAPHAYKPTGCHKPEKQQKILDVANKQPGSQGEPVPSSALLGLQSKLKPPTHQQQVSGSGAGTSGSQKPSNVAQLLSSPPELISLHRRQTSGAAAGSSSFLQGKRLQLPRSGAGPSGAGTGTGAGAAGSRSAGGPPPPNVVILPDALTPQERHESKSWKPTLIPLEDQHKVPNKSHALYQTADGRRLPALVQVQSGGKPYLISIFDYNRMCILRREKLMRDQLLKSNAKPKPQNQQQQQGQTHQQQQNSAASAAAFSNMVKLAQQHTARQQLQQLQQKPQQQQQLPTLQPGGVRLARLPQKLLMPPLTNPQIGSQAPNLQPLLSSTLDNSNNCWLWKNFPDPNQYLLNGNGGGAGSSSSKLPHLTAKPATATSSSGAANKSAGSLFTLKQQQHQQKLIDNAIMSKIPKSLTVIPQQMGGNTGGDMGGSSSSGKD

Target ORF information:

RefSeq Version	NM_001201601.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201601.2

ORF Insert Sequence:

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCAACGAGG AGAAGCTCAC CGTGGATAAC TGCTTGTGCG ACGCATGTTT TCGACACGTG 
GACCGCCGGG CAAATGTGCC ATCCTATAAG AAGCGTCTTT CCGCTTCAGG TCACTTGGAG 
ATGGGGTCTG CAGCGGGATC TGCACTAGAG AAACAGTTTG CTGGCGACAG CGGCGTCATT 
ACGGAATCGG GTGGCGAAGC TGGTTCTACG GCAGCTGTGG CCGTGCAGCA ACGATCTTGT 
GGCGTGAAGG ACTGCGTCGA AGCGGCACGA CACTCGCTGC GGCGCAAGTG CATACGCAAG 
AGAGTAAAGA AGTATCAGCT CAGCCTGGAG ATTCCCGCAG GCTCGTCGAA CGTGGGGCTG 
TGTGAGGCAC ATTACAATAC GGTCATCCAA TTTTCCGGCT GCGTTCTTTG CAAGCGTAGA 
TTAGGCAAGA ACCATATGTA CAACATAACC ACGCAGGACA CAATTCGACT GGAAAAGGCG 
CTGTCCGAGA TGGGCATCCC AGTTCAGCTT GGCATGGGCA CTGCAGTCTG CAAGCTGTGT 
CGCTATTTTG CCAACCTTTT GATAAAGCCA CCGGATAGCA CCAAGGCACA AAAGGCGGAA 
TTCGTGAAGA ACTACAGAAA GAGGCTCCTC AAGGTGCATA ATCTGCAGGA TGGCAGTCAT 
GAGCTGTCCG AAGCGGATGA AGAAGAGGCA CCTAATGCAA CGGAGACAGA AAGGCCAACC 
TCAGACGGAC ACGAAGATCC CGAGATGCCC ATGGTAGCGG ACTATGATGG ACCTACCGAC 
TCCAATTCCA GTAGTTCTTC GACTGCAGCC CTGGACACCA GCAAACAAAT GTCCAAGCTT 
CAGGCCATCC TGCAGCAAAA TGTGGGAGCG GATGCGGCAG GAGCTGCGGG AACAGGAACT 
GTTGCAGCAA GTCCCGGAGG AAGCGGATCT GGGGCAGATA TCTCTAACGT ATTGCGAGGG 
AATCCGAACA TTTCCATGCG CGAACTTTTC CACGGCGAGG AAGAGCTGGG TGTGCAGTTC 
AAGGTGCCGT TCGGATGCAG CAGCAGCCAG CGTACTCCGG AGGGCTGGAC ACGAGTGCAG 
ACTTTCCTAC AATACGATGA GCCGACGCGC CGCCTCTGGG AGGAGTTGCA AAAGCCGTAC 
GGAAATCAGA GCTCATTTCT GCGCCACTTG ATACTATTAG AGAAGTACTA CCGAAACGGA 
GATCTCGTCC TAGCACCGCA TGCTTCCTCC AATGCCACGG TTTACACAGA GACTGTTCGT 
CAGCGGCTGA ATTCGTTTGA TCACGGTCAC TGCGGTGGAT TGAACATCGC AGGCAGCCCT 
TCTTCTTCGG GTTCCGGCAA GCGCAGTGGA GTTCCTCAAC CTACGGGTGC CAGTGTGCTG 
GCCACCGCCC TCACAACACC CTTGACAAGC CATTCATCCT CCTCTGCATC CATTTCCTCC 
GAACAGCATT CGTCGGTTGA TCCTGTCATT CCGCTGGTAG ACCTCAATGA TGACGATGAA 
GGCGAAGATG GGGCAGGAGG AGCGGGCGAA AGGGAGTCGA CAAATAGGCA GCAGGACGTA 
ATCTTGGAAT GCCTTAGAAC TGCCTCTGTG GACAAGCTGA CTAAGCAGCT CAGCTCGAAT 
GCGGTGACGA TTATCGCCCG GCCCAAAGAC AAATCGCAGC TCTCCTGCAA CAGCGGATCC 
TCCACGTCCA TTTCCAGCTC CTCGTCCGCT ATTTCCTCGC CGGAGGAAGT GGCCGTCACT 
AAGGTTACAG CAGTCGCACC AGTCCAGTCC AAGGATGCAC CGCCACTGGC GCCAGCAAGT 
AGCGGTGTTA GCAACAGTCG TAGTATCCTT AAAACCAACC TCTTGGGCAT GAACAAGGCC 
GTGGAACTCG TGCCCTTAAC GACTGCCCCC CACGCTTACA AGCCAACTGG ATGCCATAAG 
CCTGAGAAAC AGCAAAAGAT TCTTGACGTG GCCAATAAGC AGCCCGGTAG CCAGGGGGAA 
CCGGTACCAT CAAGCGCCTT GCTTGGCCTG CAGTCAAAGC TAAAGCCTCC AACGCATCAG 
CAGCAGGTCA GCGGATCAGG AGCGGGAACT AGTGGTTCTC AGAAGCCATC TAATGTGGCG 
CAATTGCTTA GCTCTCCACC GGAGCTAATC AGCTTGCATC GACGGCAGAC CAGCGGAGCA 
GCAGCGGGGT CCAGCAGCTT CCTTCAGGGC AAGAGGCTTC AACTTCCACG ATCTGGAGCA 
GGGCCTTCAG GAGCGGGAAC GGGAACAGGC GCTGGAGCAG CAGGAAGCCG CAGTGCGGGT 
GGACCACCAC CGCCCAATGT GGTCATACTG CCGGACGCCT TAACCCCCCA GGAGCGACAC 
GAGAGCAAGA GCTGGAAGCC AACGCTGATA CCGCTGGAGG ATCAGCACAA GGTGCCGAAC 
AAATCACATG CTCTTTATCA GACCGCCGAC GGTCGAAGGT TGCCCGCCCT GGTGCAAGTG 
CAGTCTGGTG GCAAGCCATA CCTCATCTCT ATCTTCGACT ATAACCGCAT GTGCATCTTG 
CGAAGGGAAA AGCTGATGCG GGACCAGTTG CTCAAGAGTA ACGCCAAGCC AAAGCCGCAG 
AACCAGCAAC AGCAGCAGGG CCAAACGCAC CAGCAGCAGC AGAATTCCGC CGCATCGGCG 
GCTGCCTTCT CCAACATGGT GAAGTTGGCC CAGCAACACA CGGCGCGACA GCAGCTTCAG 
CAGCTGCAAC AGAAGCCACA ACAGCAGCAA CAATTGCCCA CTTTGCAGCC AGGTGGGGTG 
CGACTTGCCC GGCTGCCGCA AAAACTACTG ATGCCACCAC TGACTAATCC GCAGATTGGC 
AGTCAAGCAC CCAACTTACA GCCGTTGCTG TCTAGTACGC TGGATAACAG CAACAACTGC 
TGGCTGTGGA AAAACTTTCC TGATCCCAAT CAGTATCTGC TAAATGGAAA CGGAGGGGGT 
GCCGGGAGCT CCTCCAGCAA GTTGCCACAT CTCACGGCCA AACCAGCCAC GGCAACTAGT 
AGCTCCGGAG CGGCCAACAA ATCAGCAGGA AGCCTATTTA CCCTCAAGCA GCAGCAGCAC 
CAGCAGAAAC TCATCGACAA CGCTATCATG TCAAAGATAC CCAAAAGTCT GACAGTAATA 
CCGCAGCAGA TGGGTGGTAA TACCGGTGGC GATATGGGGG GCAGCAGCTC CTCCGGCAAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

east ( NM_080125.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524864.2 Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant B, mRNA.

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CloneID	OFa02400
Clone ID Related Accession (Same CDS sequence)	NM_001201602.2 , NM_001201601.2 , NM_080125.5
Accession Version	NM_080125.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhanced adult sensory threshold, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080125.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCAACGAGG AGAAGCTCAC CGTGGATAAC TGCTTGTGCG ACGCATGTTT TCGACACGTG 
GACCGCCGGG CAAATGTGCC ATCCTATAAG AAGCGTCTTT CCGCTTCAGG TCACTTGGAG 
ATGGGGTCTG CAGCGGGATC TGCACTAGAG AAACAGTTTG CTGGCGACAG CGGCGTCATT 
ACGGAATCGG GTGGCGAAGC TGGTTCTACG GCAGCTGTGG CCGTGCAGCA ACGATCTTGT 
GGCGTGAAGG ACTGCGTCGA AGCGGCACGA CACTCGCTGC GGCGCAAGTG CATACGCAAG 
AGAGTAAAGA AGTATCAGCT CAGCCTGGAG ATTCCCGCAG GCTCGTCGAA CGTGGGGCTG 
TGTGAGGCAC ATTACAATAC GGTCATCCAA TTTTCCGGCT GCGTTCTTTG CAAGCGTAGA 
TTAGGCAAGA ACCATATGTA CAACATAACC ACGCAGGACA CAATTCGACT GGAAAAGGCG 
CTGTCCGAGA TGGGCATCCC AGTTCAGCTT GGCATGGGCA CTGCAGTCTG CAAGCTGTGT 
CGCTATTTTG CCAACCTTTT GATAAAGCCA CCGGATAGCA CCAAGGCACA AAAGGCGGAA 
TTCGTGAAGA ACTACAGAAA GAGGCTCCTC AAGGTGCATA ATCTGCAGGA TGGCAGTCAT 
GAGCTGTCCG AAGCGGATGA AGAAGAGGCA CCTAATGCAA CGGAGACAGA AAGGCCAACC 
TCAGACGGAC ACGAAGATCC CGAGATGCCC ATGGTAGCGG ACTATGATGG ACCTACCGAC 
TCCAATTCCA GTAGTTCTTC GACTGCAGCC CTGGACACCA GCAAACAAAT GTCCAAGCTT 
CAGGCCATCC TGCAGCAAAA TGTGGGAGCG GATGCGGCAG GAGCTGCGGG AACAGGAACT 
GTTGCAGCAA GTCCCGGAGG AAGCGGATCT GGGGCAGATA TCTCTAACGT ATTGCGAGGG 
AATCCGAACA TTTCCATGCG CGAACTTTTC CACGGCGAGG AAGAGCTGGG TGTGCAGTTC 
AAGGTGCCGT TCGGATGCAG CAGCAGCCAG CGTACTCCGG AGGGCTGGAC ACGAGTGCAG 
ACTTTCCTAC AATACGATGA GCCGACGCGC CGCCTCTGGG AGGAGTTGCA AAAGCCGTAC 
GGAAATCAGA GCTCATTTCT GCGCCACTTG ATACTATTAG AGAAGTACTA CCGAAACGGA 
GATCTCGTCC TAGCACCGCA TGCTTCCTCC AATGCCACGG TTTACACAGA GACTGTTCGT 
CAGCGGCTGA ATTCGTTTGA TCACGGTCAC TGCGGTGGAT TGAACATCGC AGGCAGCCCT 
TCTTCTTCGG GTTCCGGCAA GCGCAGTGGA GTTCCTCAAC CTACGGGTGC CAGTGTGCTG 
GCCACCGCCC TCACAACACC CTTGACAAGC CATTCATCCT CCTCTGCATC CATTTCCTCC 
GAACAGCATT CGTCGGTTGA TCCTGTCATT CCGCTGGTAG ACCTCAATGA TGACGATGAA 
GGCGAAGATG GGGCAGGAGG AGCGGGCGAA AGGGAGTCGA CAAATAGGCA GCAGGACGTA 
ATCTTGGAAT GCCTTAGAAC TGCCTCTGTG GACAAGCTGA CTAAGCAGCT CAGCTCGAAT 
GCGGTGACGA TTATCGCCCG GCCCAAAGAC AAATCGCAGC TCTCCTGCAA CAGCGGATCC 
TCCACGTCCA TTTCCAGCTC CTCGTCCGCT ATTTCCTCGC CGGAGGAAGT GGCCGTCACT 
AAGGTTACAG CAGTCGCACC AGTCCAGTCC AAGGATGCAC CGCCACTGGC GCCAGCAAGT 
AGCGGTGTTA GCAACAGTCG TAGTATCCTT AAAACCAACC TCTTGGGCAT GAACAAGGCC 
GTGGAACTCG TGCCCTTAAC GACTGCCCCC CACGCTTACA AGCCAACTGG ATGCCATAAG 
CCTGAGAAAC AGCAAAAGAT TCTTGACGTG GCCAATAAGC AGCCCGGTAG CCAGGGGGAA 
CCGGTACCAT CAAGCGCCTT GCTTGGCCTG CAGTCAAAGC TAAAGCCTCC AACGCATCAG 
CAGCAGGTCA GCGGATCAGG AGCGGGAACT AGTGGTTCTC AGAAGCCATC TAATGTGGCG 
CAATTGCTTA GCTCTCCACC GGAGCTAATC AGCTTGCATC GACGGCAGAC CAGCGGAGCA 
GCAGCGGGGT CCAGCAGCTT CCTTCAGGGC AAGAGGCTTC AACTTCCACG ATCTGGAGCA 
GGGCCTTCAG GAGCGGGAAC GGGAACAGGC GCTGGAGCAG CAGGAAGCCG CAGTGCGGGT 
GGACCACCAC CGCCCAATGT GGTCATACTG CCGGACGCCT TAACCCCCCA GGAGCGACAC 
GAGAGCAAGA GCTGGAAGCC AACGCTGATA CCGCTGGAGG ATCAGCACAA GGTGCCGAAC 
AAATCACATG CTCTTTATCA GACCGCCGAC GGTCGAAGGT TGCCCGCCCT GGTGCAAGTG 
CAGTCTGGTG GCAAGCCATA CCTCATCTCT ATCTTCGACT ATAACCGCAT GTGCATCTTG 
CGAAGGGAAA AGCTGATGCG GGACCAGTTG CTCAAGAGTA ACGCCAAGCC AAAGCCGCAG 
AACCAGCAAC AGCAGCAGGG CCAAACGCAC CAGCAGCAGC AGAATTCCGC CGCATCGGCG 
GCTGCCTTCT CCAACATGGT GAAGTTGGCC CAGCAACACA CGGCGCGACA GCAGCTTCAG 
CAGCTGCAAC AGAAGCCACA ACAGCAGCAA CAATTGCCCA CTTTGCAGCC AGGTGGGGTG 
CGACTTGCCC GGCTGCCGCA AAAACTACTG ATGCCACCAC TGACTAATCC GCAGATTGGC 
AGTCAAGCAC CCAACTTACA GCCGTTGCTG TCTAGTACGC TGGATAACAG CAACAACTGC 
TGGCTGTGGA AAAACTTTCC TGATCCCAAT CAGTATCTGC TAAATGGAAA CGGAGGGGGT 
GCCGGGAGCT CCTCCAGCAA GTTGCCACAT CTCACGGCCA AACCAGCCAC GGCAACTAGT 
AGCTCCGGAG CGGCCAACAA ATCAGCAGGA AGCCTATTTA CCCTCAAGCA GCAGCAGCAC 
CAGCAGAAAC TCATCGACAA CGCTATCATG TCAAAGATAC CCAAAAGTCT GACAGTAATA 
CCGCAGCAGA TGGGTGGTAA TACCGGTGGC GATATGGGGG GCAGCAGCTC CTCCGGCAAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524864.2
CDS	275..7180
Translation	MSSRKVPGGSGGADESTAAAAPLDDNANASVEIPDSSEEPAMGVGEEMSIISKTRTSTLSVEPAKEPTVTAELEGEKELESNPVSKTPRSTPTPTLTPAVTPTASDGVAAKSVRVTRHSSPLLLIISPTTSRREVGDGELDTEEPTGSGGQRKSSVERSLAPVIRGRKSIKDLKEAKEVKSEEPPAAASESRAASGVTPGQVKEQHVADGNEMESLPITDKKDHKDTKDKGDERETDQEEEKEKSADTEIIADTEKTSEKQKYTEKDKAADKDGGKEKDIDANKDIDKEKEKVKEVLPPVVPIAPVTPTCNRVTRKSHAQEQAINTRVTRNRRQSSTVGANSTASLVAASSSVTEQPPPSRGRRKKPVVVAPPLEPAVKRKRSQDVEADSDANNSTKYSKVEVVKSEEAEAPEEDSSAVPIKQESVDGNEVSSISPTVTPTPTPAPTPAPVPGSRRGRGRPQNRNSSSPATTTRATRLSKAGSPVILTPVAQEPAPPKRRRVGSSTRKTVSASSLAPSSQGGAGDEDSKDSMASSMDDLLMAAADIKQEKLTPDFDDSLMPEGLPSTSGASSANGHSCTEPLTVDTEINVKPADSKVKPKESPVVAVEESPSQSETQSAKVSAHAGKAPSLSPDMISEGVSAVSVRKFYKKPEFLENNLGIEKDPELGEIVQTVSNNDTETDVEMAVDGEVNQPSTPKSQDKKKEEQEKNQKSGLKAAKKAPAKLEPKAEDISEILTDVPVDISTEAVEIIEEAEEDTCSNSSIKPGELRLDESNDEPELLLEDALIVNGDENETPDQPEEKEDQVEFFHTGEYDDFEHEIMVELAKEGVLDASGNALSQQKVELEHPEDVTLHESKNDIEAEESVERKPLKDPSVADEMEDMNEESYIDIKDQTNQLLVEHLAEEAMEADCGPEDNKENLSTSASSTAADGLDIQLAIKEDDDEEKPLAVIADEQKPGLLLTNDMKVDEKPNGKQESVCDEHVQLVPNLRQEQEIHLQNLGLLTHQAAEHRRKCLLEAQARQAQMQLQQHHHHQHKRQGARGGGSATHVESSGTLKTVIKLNRSSNGGVSGSGGLPTGTVIHGGCGSSSASSTSSSSVGSATRKSSGTLGSGAGAGAGVRRQSLKMTFQKGRARGHGAADRSADQYGAHAEDSYYTIQNENEDSSHSESQGQTDHGFYQMVKKDEKEKILIPEKASSFKFHPGRLCEDQCYYCSGKFGLYDTPCHVGQIKSVERQQKILANEEKLTVDNCLCDACFRHVDRRANVPSYKKRLSASGHLEMGSAAGSALEKQFAGDSGVITESGGEAGSTAAVAVQQRSCGVKDCVEAARHSLRRKCIRKRVKKYQLSLEIPAGSSNVGLCEAHYNTVIQFSGCVLCKRRLGKNHMYNITTQDTIRLEKALSEMGIPVQLGMGTAVCKLCRYFANLLIKPPDSTKAQKAEFVKNYRKRLLKVHNLQDGSHELSEADEEEAPNATETERPTSDGHEDPEMPMVADYDGPTDSNSSSSSTAALDTSKQMSKLQAILQQNVGADAAGAAGTGTVAASPGGSGSGADISNVLRGNPNISMRELFHGEEELGVQFKVPFGCSSSQRTPEGWTRVQTFLQYDEPTRRLWEELQKPYGNQSSFLRHLILLEKYYRNGDLVLAPHASSNATVYTETVRQRLNSFDHGHCGGLNIAGSPSSSGSGKRSGVPQPTGASVLATALTTPLTSHSSSSASISSEQHSSVDPVIPLVDLNDDDEGEDGAGGAGERESTNRQQDVILECLRTASVDKLTKQLSSNAVTIIARPKDKSQLSCNSGSSTSISSSSSAISSPEEVAVTKVTAVAPVQSKDAPPLAPASSGVSNSRSILKTNLLGMNKAVELVPLTTAPHAYKPTGCHKPEKQQKILDVANKQPGSQGEPVPSSALLGLQSKLKPPTHQQQVSGSGAGTSGSQKPSNVAQLLSSPPELISLHRRQTSGAAAGSSSFLQGKRLQLPRSGAGPSGAGTGTGAGAAGSRSAGGPPPPNVVILPDALTPQERHESKSWKPTLIPLEDQHKVPNKSHALYQTADGRRLPALVQVQSGGKPYLISIFDYNRMCILRREKLMRDQLLKSNAKPKPQNQQQQQGQTHQQQQNSAASAAAFSNMVKLAQQHTARQQLQQLQQKPQQQQQLPTLQPGGVRLARLPQKLLMPPLTNPQIGSQAPNLQPLLSSTLDNSNNCWLWKNFPDPNQYLLNGNGGGAGSSSSKLPHLTAKPATATSSSGAANKSAGSLFTLKQQQHQQKLIDNAIMSKIPKSLTVIPQQMGGNTGGDMGGSSSSGKD

Target ORF information:

RefSeq Version	NM_080125.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080125.5

ORF Insert Sequence:

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCAACGAGG AGAAGCTCAC CGTGGATAAC TGCTTGTGCG ACGCATGTTT TCGACACGTG 
GACCGCCGGG CAAATGTGCC ATCCTATAAG AAGCGTCTTT CCGCTTCAGG TCACTTGGAG 
ATGGGGTCTG CAGCGGGATC TGCACTAGAG AAACAGTTTG CTGGCGACAG CGGCGTCATT 
ACGGAATCGG GTGGCGAAGC TGGTTCTACG GCAGCTGTGG CCGTGCAGCA ACGATCTTGT 
GGCGTGAAGG ACTGCGTCGA AGCGGCACGA CACTCGCTGC GGCGCAAGTG CATACGCAAG 
AGAGTAAAGA AGTATCAGCT CAGCCTGGAG ATTCCCGCAG GCTCGTCGAA CGTGGGGCTG 
TGTGAGGCAC ATTACAATAC GGTCATCCAA TTTTCCGGCT GCGTTCTTTG CAAGCGTAGA 
TTAGGCAAGA ACCATATGTA CAACATAACC ACGCAGGACA CAATTCGACT GGAAAAGGCG 
CTGTCCGAGA TGGGCATCCC AGTTCAGCTT GGCATGGGCA CTGCAGTCTG CAAGCTGTGT 
CGCTATTTTG CCAACCTTTT GATAAAGCCA CCGGATAGCA CCAAGGCACA AAAGGCGGAA 
TTCGTGAAGA ACTACAGAAA GAGGCTCCTC AAGGTGCATA ATCTGCAGGA TGGCAGTCAT 
GAGCTGTCCG AAGCGGATGA AGAAGAGGCA CCTAATGCAA CGGAGACAGA AAGGCCAACC 
TCAGACGGAC ACGAAGATCC CGAGATGCCC ATGGTAGCGG ACTATGATGG ACCTACCGAC 
TCCAATTCCA GTAGTTCTTC GACTGCAGCC CTGGACACCA GCAAACAAAT GTCCAAGCTT 
CAGGCCATCC TGCAGCAAAA TGTGGGAGCG GATGCGGCAG GAGCTGCGGG AACAGGAACT 
GTTGCAGCAA GTCCCGGAGG AAGCGGATCT GGGGCAGATA TCTCTAACGT ATTGCGAGGG 
AATCCGAACA TTTCCATGCG CGAACTTTTC CACGGCGAGG AAGAGCTGGG TGTGCAGTTC 
AAGGTGCCGT TCGGATGCAG CAGCAGCCAG CGTACTCCGG AGGGCTGGAC ACGAGTGCAG 
ACTTTCCTAC AATACGATGA GCCGACGCGC CGCCTCTGGG AGGAGTTGCA AAAGCCGTAC 
GGAAATCAGA GCTCATTTCT GCGCCACTTG ATACTATTAG AGAAGTACTA CCGAAACGGA 
GATCTCGTCC TAGCACCGCA TGCTTCCTCC AATGCCACGG TTTACACAGA GACTGTTCGT 
CAGCGGCTGA ATTCGTTTGA TCACGGTCAC TGCGGTGGAT TGAACATCGC AGGCAGCCCT 
TCTTCTTCGG GTTCCGGCAA GCGCAGTGGA GTTCCTCAAC CTACGGGTGC CAGTGTGCTG 
GCCACCGCCC TCACAACACC CTTGACAAGC CATTCATCCT CCTCTGCATC CATTTCCTCC 
GAACAGCATT CGTCGGTTGA TCCTGTCATT CCGCTGGTAG ACCTCAATGA TGACGATGAA 
GGCGAAGATG GGGCAGGAGG AGCGGGCGAA AGGGAGTCGA CAAATAGGCA GCAGGACGTA 
ATCTTGGAAT GCCTTAGAAC TGCCTCTGTG GACAAGCTGA CTAAGCAGCT CAGCTCGAAT 
GCGGTGACGA TTATCGCCCG GCCCAAAGAC AAATCGCAGC TCTCCTGCAA CAGCGGATCC 
TCCACGTCCA TTTCCAGCTC CTCGTCCGCT ATTTCCTCGC CGGAGGAAGT GGCCGTCACT 
AAGGTTACAG CAGTCGCACC AGTCCAGTCC AAGGATGCAC CGCCACTGGC GCCAGCAAGT 
AGCGGTGTTA GCAACAGTCG TAGTATCCTT AAAACCAACC TCTTGGGCAT GAACAAGGCC 
GTGGAACTCG TGCCCTTAAC GACTGCCCCC CACGCTTACA AGCCAACTGG ATGCCATAAG 
CCTGAGAAAC AGCAAAAGAT TCTTGACGTG GCCAATAAGC AGCCCGGTAG CCAGGGGGAA 
CCGGTACCAT CAAGCGCCTT GCTTGGCCTG CAGTCAAAGC TAAAGCCTCC AACGCATCAG 
CAGCAGGTCA GCGGATCAGG AGCGGGAACT AGTGGTTCTC AGAAGCCATC TAATGTGGCG 
CAATTGCTTA GCTCTCCACC GGAGCTAATC AGCTTGCATC GACGGCAGAC CAGCGGAGCA 
GCAGCGGGGT CCAGCAGCTT CCTTCAGGGC AAGAGGCTTC AACTTCCACG ATCTGGAGCA 
GGGCCTTCAG GAGCGGGAAC GGGAACAGGC GCTGGAGCAG CAGGAAGCCG CAGTGCGGGT 
GGACCACCAC CGCCCAATGT GGTCATACTG CCGGACGCCT TAACCCCCCA GGAGCGACAC 
GAGAGCAAGA GCTGGAAGCC AACGCTGATA CCGCTGGAGG ATCAGCACAA GGTGCCGAAC 
AAATCACATG CTCTTTATCA GACCGCCGAC GGTCGAAGGT TGCCCGCCCT GGTGCAAGTG 
CAGTCTGGTG GCAAGCCATA CCTCATCTCT ATCTTCGACT ATAACCGCAT GTGCATCTTG 
CGAAGGGAAA AGCTGATGCG GGACCAGTTG CTCAAGAGTA ACGCCAAGCC AAAGCCGCAG 
AACCAGCAAC AGCAGCAGGG CCAAACGCAC CAGCAGCAGC AGAATTCCGC CGCATCGGCG 
GCTGCCTTCT CCAACATGGT GAAGTTGGCC CAGCAACACA CGGCGCGACA GCAGCTTCAG 
CAGCTGCAAC AGAAGCCACA ACAGCAGCAA CAATTGCCCA CTTTGCAGCC AGGTGGGGTG 
CGACTTGCCC GGCTGCCGCA AAAACTACTG ATGCCACCAC TGACTAATCC GCAGATTGGC 
AGTCAAGCAC CCAACTTACA GCCGTTGCTG TCTAGTACGC TGGATAACAG CAACAACTGC 
TGGCTGTGGA AAAACTTTCC TGATCCCAAT CAGTATCTGC TAAATGGAAA CGGAGGGGGT 
GCCGGGAGCT CCTCCAGCAA GTTGCCACAT CTCACGGCCA AACCAGCCAC GGCAACTAGT 
AGCTCCGGAG CGGCCAACAA ATCAGCAGGA AGCCTATTTA CCCTCAAGCA GCAGCAGCAC 
CAGCAGAAAC TCATCGACAA CGCTATCATG TCAAAGATAC CCAAAAGTCT GACAGTAATA 
CCGCAGCAGA TGGGTGGTAA TACCGGTGGC GATATGGGGG GCAGCAGCTC CTCCGGCAAG 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

east ( NM_001258555.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245484.1 Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant E, mRNA.

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CloneID	OFa02753
Clone ID Related Accession (Same CDS sequence)	NM_001258555.1 , NM_001258556.2
Accession Version	NM_001258555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhanced adult sensory threshold, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG GTGCGAAAAA GTTTGTTGTA ACTACTGGTA ATACCGGCCG CAAGACTAAT 
AACCGTTTCA GCTCAACTAA CAACTACCAC TCGACGGTAG CCTTGCACGG TAGCAACTCT 
GCGCTCCAGT ACTATTCGTC CCACTCGGAA AGTCAGGGAC AGACGGACCA CGGCTTCTAT 
CAGATGGTCA AAAAGGACGA AAAGGAGAAG ATCCTCATTC CGGAAAAGGC CTCCTCGTTT 
AAGTTTCACC CAGGGAGACT GTGCGAAGAC CAGTGCTACT ACTGTAGCGG AAAGTTTGGC 
CTCTATGACA CCCCCTGCCA TGTTGGACAA ATAAAGTCCG TGGAGCGCCA GCAGAAGATC 
CTAGCCAACG AGGAGAAGCT CACCGTGGAT AACTGCTTGT GCGACGCATG TTTTCGACAC 
GTGGACCGCC GGGCAAATGT GCCATCCTAT AAGAAGCGTC TTTCCGCTTC AGGTCACTTG 
GAGATGGGGT CTGCAGCGGG ATCTGCACTA GAGAAACAGT TTGCTGGCGA CAGCGGCGTC 
ATTACGGAAT CGGGTGGCGA AGCTGGTTCT ACGGCAGCTG TGGCCGTGCA GCAACGATCT 
TGTGGCGTGA AGGACTGCGT CGAAGCGGCA CGACACTCGC TGCGGCGCAA GTGCATACGC 
AAGAGAGTAA AGAAGTATCA GCTCAGCCTG GAGATTCCCG CAGGCTCGTC GAACGTGGGG 
CTGTGTGAGG CACATTACAA TACGGTCATC CAATTTTCCG GCTGCGTTCT TTGCAAGCGT 
AGATTAGGCA AGAACCATAT GTACAACATA ACCACGCAGG ACACAATTCG ACTGGAAAAG 
GCGCTGTCCG AGATGGGCAT CCCAGTTCAG CTTGGCATGG GCACTGCAGT CTGCAAGCTG 
TGTCGCTATT TTGCCAACCT TTTGATAAAG CCACCGGATA GCACCAAGGC ACAAAAGGCG 
GAATTCGTGA AGAACTACAG AAAGAGGCTC CTCAAGGTGC ATAATCTGCA GGATGGCAGT 
CATGAGCTGT CCGAAGCGGA TGAAGAAGAG GCACCTAATG CAACGGAGAC AGAAAGGCCA 
ACCTCAGACG GACACGAAGA TCCCGAGATG CCCATGGTAG CGGACTATGA TGGACCTACC 
GACTCCAATT CCAGTAGTTC TTCGACTGCA GCCCTGGACA CCAGCAAACA AATGTCCAAG 
CTTCAGGCCA TCCTGCAGCA AAATGTGGGA GCGGATGCGG CAGGAGCTGC GGGAACAGGA 
ACTGTTGCAG CAAGTCCCGG AGGAAGCGGA TCTGGGGCAG ATATCTCTAA CGTATTGCGA 
GGGAATCCGA ACATTTCCAT GCGCGAACTT TTCCACGGCG AGGAAGAGCT GGGTGTGCAG 
TTCAAGGTGC CGTTCGGATG CAGCAGCAGC CAGCGTACTC CGGAGGGCTG GACACGAGTG 
CAGACTTTCC TACAATACGA TGAGCCGACG CGCCGCCTCT GGGAGGAGTT GCAAAAGCCG 
TACGGAAATC AGAGCTCATT TCTGCGCCAC TTGATACTAT TAGAGAAGTA CTACCGAAAC 
GGAGATCTCG TCCTAGCACC GCATGCTTCC TCCAATGCCA CGGTTTACAC AGAGACTGTT 
CGTCAGCGGC TGAATTCGTT TGATCACGGT CACTGCGGTG GATTGAACAT CGCAGGCAGC 
CCTTCTTCTT CGGGTTCCGG CAAGCGCAGT GGAGTTCCTC AACCTACGGG TGCCAGTGTG 
CTGGCCACCG CCCTCACAAC ACCCTTGACA AGCCATTCAT CCTCCTCTGC ATCCATTTCC 
TCCGAACAGC ATTCGTCGGT TGATCCTGTC ATTCCGCTGG TAGACCTCAA TGATGACGAT 
GAAGGCGAAG ATGGGGCAGG AGGAGCGGGC GAAAGGGAGT CGACAAATAG GCAGCAGGAC 
GTAATCTTGG AATGCCTTAG AACTGCCTCT GTGGACAAGC TGACTAAGCA GCTCAGCTCG 
AATGCGGTGA CGATTATCGC CCGGCCCAAA GACAAATCGC AGCTCTCCTG CAACAGCGGA 
TCCTCCACGT CCATTTCCAG CTCCTCGTCC GCTATTTCCT CGCCGGAGGA AGTGGCCGTC 
ACTAAGGTTA CAGCAGTCGC ACCAGTCCAG TCCAAGGATG CACCGCCACT GGCGCCAGCA 
AGTAGCGGTG TTAGCAACAG TCGTAGTATC CTTAAAACCA ACCTCTTGGG CATGAACAAG 
GCCGTGGAAC TCGTGCCCTT AACGACTGCC CCCCACGCTT ACAAGCCAAC TGGATGCCAT 
AAGCCTGAGA AACAGCAAAA GATTCTTGAC GTGGCCAATA AGCAGCCCGG TAGCCAGGGG 
GAACCGGTAC CATCAAGCGC CTTGCTTGGC CTGCAGTCAA AGCTAAAGCC TCCAACGCAT 
CAGCAGCAGG TCAGCGGATC AGGAGCGGGA ACTAGTGGTT CTCAGAAGCC ATCTAATGTG 
GCGCAATTGC TTAGCTCTCC ACCGGAGCTA ATCAGCTTGC ATCGACGGCA GACCAGCGGA 
GCAGCAGCGG GGTCCAGCAG CTTCCTTCAG GGCAAGAGGC TTCAACTTCC ACGATCTGGA 
GCAGGGCCTT CAGGAGCGGG AACGGGAACA GGCGCTGGAG CAGCAGGAAG CCGCAGTGCG 
GGTGGACCAC CACCGCCCAA TGTGGTCATA CTGCCGGACG CCTTAACCCC CCAGGAGCGA 
CACGAGAGCA AGAGCTGGAA GCCAACGCTG ATACCGCTGG AGGATCAGCA CAAGGTGCCG 
AACAAATCAC ATGCTCTTTA TCAGACCGCC GACGGTCGAA GGTTGCCCGC CCTGGTGCAA 
GTGCAGTCTG GTGGCAAGCC ATACCTCATC TCTATCTTCG ACTATAACCG CATGTGCATC 
TTGCGAAGGG AAAAGCTGAT GCGGGACCAG TTGCTCAAGA GTAACGCCAA GCCAAAGCCG 
CAGAACCAGC AACAGCAGCA GGGCCAAACG CACCAGCAGC AGCAGAATTC CGCCGCATCG 
GCGGCTGCCT TCTCCAACAT GGTGAAGTTG GCCCAGCAAC ACACGGCGCG ACAGCAGCTT 
CAGCAGCTGC AACAGAAGCC ACAACAGCAG CAACAATTGC CCACTTTGCA GCCAGGTGGG 
GTGCGACTTG CCCGGCTGCC GCAAAAACTA CTGATGCCAC CACTGACTAA TCCGCAGATT 
GGCAGTCAAG CACCCAACTT ACAGCCGTTG CTGTCTAGTA CGCTGGATAA CAGCAACAAC 
TGCTGGCTGT GGAAAAACTT TCCTGATCCC AATCAGTATC TGCTAAATGG AAACGGAGGG 
GGTGCCGGGA GCTCCTCCAG CAAGTTGCCA CATCTCACGG CCAAACCAGC CACGGCAACT 
AGTAGCTCCG GAGCGGCCAA CAAATCAGCA GGAAGCCTAT TTACCCTCAA GCAGCAGCAG 
CACCAGCAGA AACTCATCGA CAACGCTATC ATGTCAAAGA TACCCAAAAG TCTGACAGTA 
ATACCGCAGC AGATGGGTGG TAATACCGGT GGCGATATGG GGGGCAGCAG CTCCTCCGGC 
AAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245484.1
CDS	282..7310
Translation	MSSRKVPGGSGGADESTAAAAPLDDNANASVEIPDSSEEPAMGVGEEMSIISKTRTSTLSVEPAKEPTVTAELEGEKELESNPVSKTPRSTPTPTLTPAVTPTASDGVAAKSVRVTRHSSPLLLIISPTTSRREVGDGELDTEEPTGSGGQRKSSVERSLAPVIRGRKSIKDLKEAKEVKSEEPPAAASESRAASGVTPGQVKEQHVADGNEMESLPITDKKDHKDTKDKGDERETDQEEEKEKSADTEIIADTEKTSEKQKYTEKDKAADKDGGKEKDIDANKDIDKEKEKVKEVLPPVVPIAPVTPTCNRVTRKSHAQEQAINTRVTRNRRQSSTVGANSTASLVAASSSVTEQPPPSRGRRKKPVVVAPPLEPAVKRKRSQDVEADSDANNSTKYSKVEVVKSEEAEAPEEDSSAVPIKQESVDGNEVSSISPTVTPTPTPAPTPAPVPGSRRGRGRPQNRNSSSPATTTRATRLSKAGSPVILTPVAQEPAPPKRRRVGSSTRKTVSASSLAPSSQGGAGDEDSKDSMASSMDDLLMAAADIKQEKLTPDFDDSLMPEGLPSTSGASSANGHSCTEPLTVDTEINVKPADSKVKPKESPVVAVEESPSQSETQSAKVSAHAGKAPSLSPDMISEGVSAVSVRKFYKKPEFLENNLGIEKDPELGEIVQTVSNNDTETDVEMAVDGEVNQPSTPKSQDKKKEEQEKNQKSGLKAAKKAPAKLEPKAEDISEILTDVPVDISTEAVEIIEEAEEDTCSNSSIKPGELRLDESNDEPELLLEDALIVNGDENETPDQPEEKEDQVEFFHTGEYDDFEHEIMVELAKEGVLDASGNALSQQKVELEHPEDVTLHESKNDIEAEESVERKPLKDPSVADEMEDMNEESYIDIKDQTNQLLVEHLAEEAMEADCGPEDNKENLSTSASSTAADGLDIQLAIKEDDDEEKPLAVIADEQKPGLLLTNDMKVDEKPNGKQESVCDEHVQLVPNLRQEQEIHLQNLGLLTHQAAEHRRKCLLEAQARQAQMQLQQHHHHQHKRQGARGGGSATHVESSGTLKTVIKLNRSSNGGVSGSGGLPTGTVIHGGCGSSSASSTSSSSVGSATRKSSGTLGSGAGAGAGVRRQSLKMTFQKGRARGHGAADRSADQYGAHAEDSYYTIQNENEGAKKFVVTTGNTGRKTNNRFSSTNNYHSTVALHGSNSALQYYSSHSESQGQTDHGFYQMVKKDEKEKILIPEKASSFKFHPGRLCEDQCYYCSGKFGLYDTPCHVGQIKSVERQQKILANEEKLTVDNCLCDACFRHVDRRANVPSYKKRLSASGHLEMGSAAGSALEKQFAGDSGVITESGGEAGSTAAVAVQQRSCGVKDCVEAARHSLRRKCIRKRVKKYQLSLEIPAGSSNVGLCEAHYNTVIQFSGCVLCKRRLGKNHMYNITTQDTIRLEKALSEMGIPVQLGMGTAVCKLCRYFANLLIKPPDSTKAQKAEFVKNYRKRLLKVHNLQDGSHELSEADEEEAPNATETERPTSDGHEDPEMPMVADYDGPTDSNSSSSSTAALDTSKQMSKLQAILQQNVGADAAGAAGTGTVAASPGGSGSGADISNVLRGNPNISMRELFHGEEELGVQFKVPFGCSSSQRTPEGWTRVQTFLQYDEPTRRLWEELQKPYGNQSSFLRHLILLEKYYRNGDLVLAPHASSNATVYTETVRQRLNSFDHGHCGGLNIAGSPSSSGSGKRSGVPQPTGASVLATALTTPLTSHSSSSASISSEQHSSVDPVIPLVDLNDDDEGEDGAGGAGERESTNRQQDVILECLRTASVDKLTKQLSSNAVTIIARPKDKSQLSCNSGSSTSISSSSSAISSPEEVAVTKVTAVAPVQSKDAPPLAPASSGVSNSRSILKTNLLGMNKAVELVPLTTAPHAYKPTGCHKPEKQQKILDVANKQPGSQGEPVPSSALLGLQSKLKPPTHQQQVSGSGAGTSGSQKPSNVAQLLSSPPELISLHRRQTSGAAAGSSSFLQGKRLQLPRSGAGPSGAGTGTGAGAAGSRSAGGPPPPNVVILPDALTPQERHESKSWKPTLIPLEDQHKVPNKSHALYQTADGRRLPALVQVQSGGKPYLISIFDYNRMCILRREKLMRDQLLKSNAKPKPQNQQQQQGQTHQQQQNSAASAAAFSNMVKLAQQHTARQQLQQLQQKPQQQQQLPTLQPGGVRLARLPQKLLMPPLTNPQIGSQAPNLQPLLSSTLDNSNNCWLWKNFPDPNQYLLNGNGGGAGSSSSKLPHLTAKPATATSSSGAANKSAGSLFTLKQQQHQQKLIDNAIMSKIPKSLTVIPQQMGGNTGGDMGGSSSSGKD

Target ORF information:

RefSeq Version	NM_001258555.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258555.1

ORF Insert Sequence:

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG GTGCGAAAAA GTTTGTTGTA ACTACTGGTA ATACCGGCCG CAAGACTAAT 
AACCGTTTCA GCTCAACTAA CAACTACCAC TCGACGGTAG CCTTGCACGG TAGCAACTCT 
GCGCTCCAGT ACTATTCGTC CCACTCGGAA AGTCAGGGAC AGACGGACCA CGGCTTCTAT 
CAGATGGTCA AAAAGGACGA AAAGGAGAAG ATCCTCATTC CGGAAAAGGC CTCCTCGTTT 
AAGTTTCACC CAGGGAGACT GTGCGAAGAC CAGTGCTACT ACTGTAGCGG AAAGTTTGGC 
CTCTATGACA CCCCCTGCCA TGTTGGACAA ATAAAGTCCG TGGAGCGCCA GCAGAAGATC 
CTAGCCAACG AGGAGAAGCT CACCGTGGAT AACTGCTTGT GCGACGCATG TTTTCGACAC 
GTGGACCGCC GGGCAAATGT GCCATCCTAT AAGAAGCGTC TTTCCGCTTC AGGTCACTTG 
GAGATGGGGT CTGCAGCGGG ATCTGCACTA GAGAAACAGT TTGCTGGCGA CAGCGGCGTC 
ATTACGGAAT CGGGTGGCGA AGCTGGTTCT ACGGCAGCTG TGGCCGTGCA GCAACGATCT 
TGTGGCGTGA AGGACTGCGT CGAAGCGGCA CGACACTCGC TGCGGCGCAA GTGCATACGC 
AAGAGAGTAA AGAAGTATCA GCTCAGCCTG GAGATTCCCG CAGGCTCGTC GAACGTGGGG 
CTGTGTGAGG CACATTACAA TACGGTCATC CAATTTTCCG GCTGCGTTCT TTGCAAGCGT 
AGATTAGGCA AGAACCATAT GTACAACATA ACCACGCAGG ACACAATTCG ACTGGAAAAG 
GCGCTGTCCG AGATGGGCAT CCCAGTTCAG CTTGGCATGG GCACTGCAGT CTGCAAGCTG 
TGTCGCTATT TTGCCAACCT TTTGATAAAG CCACCGGATA GCACCAAGGC ACAAAAGGCG 
GAATTCGTGA AGAACTACAG AAAGAGGCTC CTCAAGGTGC ATAATCTGCA GGATGGCAGT 
CATGAGCTGT CCGAAGCGGA TGAAGAAGAG GCACCTAATG CAACGGAGAC AGAAAGGCCA 
ACCTCAGACG GACACGAAGA TCCCGAGATG CCCATGGTAG CGGACTATGA TGGACCTACC 
GACTCCAATT CCAGTAGTTC TTCGACTGCA GCCCTGGACA CCAGCAAACA AATGTCCAAG 
CTTCAGGCCA TCCTGCAGCA AAATGTGGGA GCGGATGCGG CAGGAGCTGC GGGAACAGGA 
ACTGTTGCAG CAAGTCCCGG AGGAAGCGGA TCTGGGGCAG ATATCTCTAA CGTATTGCGA 
GGGAATCCGA ACATTTCCAT GCGCGAACTT TTCCACGGCG AGGAAGAGCT GGGTGTGCAG 
TTCAAGGTGC CGTTCGGATG CAGCAGCAGC CAGCGTACTC CGGAGGGCTG GACACGAGTG 
CAGACTTTCC TACAATACGA TGAGCCGACG CGCCGCCTCT GGGAGGAGTT GCAAAAGCCG 
TACGGAAATC AGAGCTCATT TCTGCGCCAC TTGATACTAT TAGAGAAGTA CTACCGAAAC 
GGAGATCTCG TCCTAGCACC GCATGCTTCC TCCAATGCCA CGGTTTACAC AGAGACTGTT 
CGTCAGCGGC TGAATTCGTT TGATCACGGT CACTGCGGTG GATTGAACAT CGCAGGCAGC 
CCTTCTTCTT CGGGTTCCGG CAAGCGCAGT GGAGTTCCTC AACCTACGGG TGCCAGTGTG 
CTGGCCACCG CCCTCACAAC ACCCTTGACA AGCCATTCAT CCTCCTCTGC ATCCATTTCC 
TCCGAACAGC ATTCGTCGGT TGATCCTGTC ATTCCGCTGG TAGACCTCAA TGATGACGAT 
GAAGGCGAAG ATGGGGCAGG AGGAGCGGGC GAAAGGGAGT CGACAAATAG GCAGCAGGAC 
GTAATCTTGG AATGCCTTAG AACTGCCTCT GTGGACAAGC TGACTAAGCA GCTCAGCTCG 
AATGCGGTGA CGATTATCGC CCGGCCCAAA GACAAATCGC AGCTCTCCTG CAACAGCGGA 
TCCTCCACGT CCATTTCCAG CTCCTCGTCC GCTATTTCCT CGCCGGAGGA AGTGGCCGTC 
ACTAAGGTTA CAGCAGTCGC ACCAGTCCAG TCCAAGGATG CACCGCCACT GGCGCCAGCA 
AGTAGCGGTG TTAGCAACAG TCGTAGTATC CTTAAAACCA ACCTCTTGGG CATGAACAAG 
GCCGTGGAAC TCGTGCCCTT AACGACTGCC CCCCACGCTT ACAAGCCAAC TGGATGCCAT 
AAGCCTGAGA AACAGCAAAA GATTCTTGAC GTGGCCAATA AGCAGCCCGG TAGCCAGGGG 
GAACCGGTAC CATCAAGCGC CTTGCTTGGC CTGCAGTCAA AGCTAAAGCC TCCAACGCAT 
CAGCAGCAGG TCAGCGGATC AGGAGCGGGA ACTAGTGGTT CTCAGAAGCC ATCTAATGTG 
GCGCAATTGC TTAGCTCTCC ACCGGAGCTA ATCAGCTTGC ATCGACGGCA GACCAGCGGA 
GCAGCAGCGG GGTCCAGCAG CTTCCTTCAG GGCAAGAGGC TTCAACTTCC ACGATCTGGA 
GCAGGGCCTT CAGGAGCGGG AACGGGAACA GGCGCTGGAG CAGCAGGAAG CCGCAGTGCG 
GGTGGACCAC CACCGCCCAA TGTGGTCATA CTGCCGGACG CCTTAACCCC CCAGGAGCGA 
CACGAGAGCA AGAGCTGGAA GCCAACGCTG ATACCGCTGG AGGATCAGCA CAAGGTGCCG 
AACAAATCAC ATGCTCTTTA TCAGACCGCC GACGGTCGAA GGTTGCCCGC CCTGGTGCAA 
GTGCAGTCTG GTGGCAAGCC ATACCTCATC TCTATCTTCG ACTATAACCG CATGTGCATC 
TTGCGAAGGG AAAAGCTGAT GCGGGACCAG TTGCTCAAGA GTAACGCCAA GCCAAAGCCG 
CAGAACCAGC AACAGCAGCA GGGCCAAACG CACCAGCAGC AGCAGAATTC CGCCGCATCG 
GCGGCTGCCT TCTCCAACAT GGTGAAGTTG GCCCAGCAAC ACACGGCGCG ACAGCAGCTT 
CAGCAGCTGC AACAGAAGCC ACAACAGCAG CAACAATTGC CCACTTTGCA GCCAGGTGGG 
GTGCGACTTG CCCGGCTGCC GCAAAAACTA CTGATGCCAC CACTGACTAA TCCGCAGATT 
GGCAGTCAAG CACCCAACTT ACAGCCGTTG CTGTCTAGTA CGCTGGATAA CAGCAACAAC 
TGCTGGCTGT GGAAAAACTT TCCTGATCCC AATCAGTATC TGCTAAATGG AAACGGAGGG 
GGTGCCGGGA GCTCCTCCAG CAAGTTGCCA CATCTCACGG CCAAACCAGC CACGGCAACT 
AGTAGCTCCG GAGCGGCCAA CAAATCAGCA GGAAGCCTAT TTACCCTCAA GCAGCAGCAG 
CACCAGCAGA AACTCATCGA CAACGCTATC ATGTCAAAGA TACCCAAAAG TCTGACAGTA 
ATACCGCAGC AGATGGGTGG TAATACCGGT GGCGATATGG GGGGCAGCAG CTCCTCCGGC 
AAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

east ( NM_001258556.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245485.1 Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant F, mRNA.

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CloneID	OFa02753
Clone ID Related Accession (Same CDS sequence)	NM_001258555.1 , NM_001258556.2
Accession Version	NM_001258556.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhanced adult sensory threshold, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258556.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG GTGCGAAAAA GTTTGTTGTA ACTACTGGTA ATACCGGCCG CAAGACTAAT 
AACCGTTTCA GCTCAACTAA CAACTACCAC TCGACGGTAG CCTTGCACGG TAGCAACTCT 
GCGCTCCAGT ACTATTCGTC CCACTCGGAA AGTCAGGGAC AGACGGACCA CGGCTTCTAT 
CAGATGGTCA AAAAGGACGA AAAGGAGAAG ATCCTCATTC CGGAAAAGGC CTCCTCGTTT 
AAGTTTCACC CAGGGAGACT GTGCGAAGAC CAGTGCTACT ACTGTAGCGG AAAGTTTGGC 
CTCTATGACA CCCCCTGCCA TGTTGGACAA ATAAAGTCCG TGGAGCGCCA GCAGAAGATC 
CTAGCCAACG AGGAGAAGCT CACCGTGGAT AACTGCTTGT GCGACGCATG TTTTCGACAC 
GTGGACCGCC GGGCAAATGT GCCATCCTAT AAGAAGCGTC TTTCCGCTTC AGGTCACTTG 
GAGATGGGGT CTGCAGCGGG ATCTGCACTA GAGAAACAGT TTGCTGGCGA CAGCGGCGTC 
ATTACGGAAT CGGGTGGCGA AGCTGGTTCT ACGGCAGCTG TGGCCGTGCA GCAACGATCT 
TGTGGCGTGA AGGACTGCGT CGAAGCGGCA CGACACTCGC TGCGGCGCAA GTGCATACGC 
AAGAGAGTAA AGAAGTATCA GCTCAGCCTG GAGATTCCCG CAGGCTCGTC GAACGTGGGG 
CTGTGTGAGG CACATTACAA TACGGTCATC CAATTTTCCG GCTGCGTTCT TTGCAAGCGT 
AGATTAGGCA AGAACCATAT GTACAACATA ACCACGCAGG ACACAATTCG ACTGGAAAAG 
GCGCTGTCCG AGATGGGCAT CCCAGTTCAG CTTGGCATGG GCACTGCAGT CTGCAAGCTG 
TGTCGCTATT TTGCCAACCT TTTGATAAAG CCACCGGATA GCACCAAGGC ACAAAAGGCG 
GAATTCGTGA AGAACTACAG AAAGAGGCTC CTCAAGGTGC ATAATCTGCA GGATGGCAGT 
CATGAGCTGT CCGAAGCGGA TGAAGAAGAG GCACCTAATG CAACGGAGAC AGAAAGGCCA 
ACCTCAGACG GACACGAAGA TCCCGAGATG CCCATGGTAG CGGACTATGA TGGACCTACC 
GACTCCAATT CCAGTAGTTC TTCGACTGCA GCCCTGGACA CCAGCAAACA AATGTCCAAG 
CTTCAGGCCA TCCTGCAGCA AAATGTGGGA GCGGATGCGG CAGGAGCTGC GGGAACAGGA 
ACTGTTGCAG CAAGTCCCGG AGGAAGCGGA TCTGGGGCAG ATATCTCTAA CGTATTGCGA 
GGGAATCCGA ACATTTCCAT GCGCGAACTT TTCCACGGCG AGGAAGAGCT GGGTGTGCAG 
TTCAAGGTGC CGTTCGGATG CAGCAGCAGC CAGCGTACTC CGGAGGGCTG GACACGAGTG 
CAGACTTTCC TACAATACGA TGAGCCGACG CGCCGCCTCT GGGAGGAGTT GCAAAAGCCG 
TACGGAAATC AGAGCTCATT TCTGCGCCAC TTGATACTAT TAGAGAAGTA CTACCGAAAC 
GGAGATCTCG TCCTAGCACC GCATGCTTCC TCCAATGCCA CGGTTTACAC AGAGACTGTT 
CGTCAGCGGC TGAATTCGTT TGATCACGGT CACTGCGGTG GATTGAACAT CGCAGGCAGC 
CCTTCTTCTT CGGGTTCCGG CAAGCGCAGT GGAGTTCCTC AACCTACGGG TGCCAGTGTG 
CTGGCCACCG CCCTCACAAC ACCCTTGACA AGCCATTCAT CCTCCTCTGC ATCCATTTCC 
TCCGAACAGC ATTCGTCGGT TGATCCTGTC ATTCCGCTGG TAGACCTCAA TGATGACGAT 
GAAGGCGAAG ATGGGGCAGG AGGAGCGGGC GAAAGGGAGT CGACAAATAG GCAGCAGGAC 
GTAATCTTGG AATGCCTTAG AACTGCCTCT GTGGACAAGC TGACTAAGCA GCTCAGCTCG 
AATGCGGTGA CGATTATCGC CCGGCCCAAA GACAAATCGC AGCTCTCCTG CAACAGCGGA 
TCCTCCACGT CCATTTCCAG CTCCTCGTCC GCTATTTCCT CGCCGGAGGA AGTGGCCGTC 
ACTAAGGTTA CAGCAGTCGC ACCAGTCCAG TCCAAGGATG CACCGCCACT GGCGCCAGCA 
AGTAGCGGTG TTAGCAACAG TCGTAGTATC CTTAAAACCA ACCTCTTGGG CATGAACAAG 
GCCGTGGAAC TCGTGCCCTT AACGACTGCC CCCCACGCTT ACAAGCCAAC TGGATGCCAT 
AAGCCTGAGA AACAGCAAAA GATTCTTGAC GTGGCCAATA AGCAGCCCGG TAGCCAGGGG 
GAACCGGTAC CATCAAGCGC CTTGCTTGGC CTGCAGTCAA AGCTAAAGCC TCCAACGCAT 
CAGCAGCAGG TCAGCGGATC AGGAGCGGGA ACTAGTGGTT CTCAGAAGCC ATCTAATGTG 
GCGCAATTGC TTAGCTCTCC ACCGGAGCTA ATCAGCTTGC ATCGACGGCA GACCAGCGGA 
GCAGCAGCGG GGTCCAGCAG CTTCCTTCAG GGCAAGAGGC TTCAACTTCC ACGATCTGGA 
GCAGGGCCTT CAGGAGCGGG AACGGGAACA GGCGCTGGAG CAGCAGGAAG CCGCAGTGCG 
GGTGGACCAC CACCGCCCAA TGTGGTCATA CTGCCGGACG CCTTAACCCC CCAGGAGCGA 
CACGAGAGCA AGAGCTGGAA GCCAACGCTG ATACCGCTGG AGGATCAGCA CAAGGTGCCG 
AACAAATCAC ATGCTCTTTA TCAGACCGCC GACGGTCGAA GGTTGCCCGC CCTGGTGCAA 
GTGCAGTCTG GTGGCAAGCC ATACCTCATC TCTATCTTCG ACTATAACCG CATGTGCATC 
TTGCGAAGGG AAAAGCTGAT GCGGGACCAG TTGCTCAAGA GTAACGCCAA GCCAAAGCCG 
CAGAACCAGC AACAGCAGCA GGGCCAAACG CACCAGCAGC AGCAGAATTC CGCCGCATCG 
GCGGCTGCCT TCTCCAACAT GGTGAAGTTG GCCCAGCAAC ACACGGCGCG ACAGCAGCTT 
CAGCAGCTGC AACAGAAGCC ACAACAGCAG CAACAATTGC CCACTTTGCA GCCAGGTGGG 
GTGCGACTTG CCCGGCTGCC GCAAAAACTA CTGATGCCAC CACTGACTAA TCCGCAGATT 
GGCAGTCAAG CACCCAACTT ACAGCCGTTG CTGTCTAGTA CGCTGGATAA CAGCAACAAC 
TGCTGGCTGT GGAAAAACTT TCCTGATCCC AATCAGTATC TGCTAAATGG AAACGGAGGG 
GGTGCCGGGA GCTCCTCCAG CAAGTTGCCA CATCTCACGG CCAAACCAGC CACGGCAACT 
AGTAGCTCCG GAGCGGCCAA CAAATCAGCA GGAAGCCTAT TTACCCTCAA GCAGCAGCAG 
CACCAGCAGA AACTCATCGA CAACGCTATC ATGTCAAAGA TACCCAAAAG TCTGACAGTA 
ATACCGCAGC AGATGGGTGG TAATACCGGT GGCGATATGG GGGGCAGCAG CTCCTCCGGC 
AAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245485.1
CDS	196..7224
Translation	MSSRKVPGGSGGADESTAAAAPLDDNANASVEIPDSSEEPAMGVGEEMSIISKTRTSTLSVEPAKEPTVTAELEGEKELESNPVSKTPRSTPTPTLTPAVTPTASDGVAAKSVRVTRHSSPLLLIISPTTSRREVGDGELDTEEPTGSGGQRKSSVERSLAPVIRGRKSIKDLKEAKEVKSEEPPAAASESRAASGVTPGQVKEQHVADGNEMESLPITDKKDHKDTKDKGDERETDQEEEKEKSADTEIIADTEKTSEKQKYTEKDKAADKDGGKEKDIDANKDIDKEKEKVKEVLPPVVPIAPVTPTCNRVTRKSHAQEQAINTRVTRNRRQSSTVGANSTASLVAASSSVTEQPPPSRGRRKKPVVVAPPLEPAVKRKRSQDVEADSDANNSTKYSKVEVVKSEEAEAPEEDSSAVPIKQESVDGNEVSSISPTVTPTPTPAPTPAPVPGSRRGRGRPQNRNSSSPATTTRATRLSKAGSPVILTPVAQEPAPPKRRRVGSSTRKTVSASSLAPSSQGGAGDEDSKDSMASSMDDLLMAAADIKQEKLTPDFDDSLMPEGLPSTSGASSANGHSCTEPLTVDTEINVKPADSKVKPKESPVVAVEESPSQSETQSAKVSAHAGKAPSLSPDMISEGVSAVSVRKFYKKPEFLENNLGIEKDPELGEIVQTVSNNDTETDVEMAVDGEVNQPSTPKSQDKKKEEQEKNQKSGLKAAKKAPAKLEPKAEDISEILTDVPVDISTEAVEIIEEAEEDTCSNSSIKPGELRLDESNDEPELLLEDALIVNGDENETPDQPEEKEDQVEFFHTGEYDDFEHEIMVELAKEGVLDASGNALSQQKVELEHPEDVTLHESKNDIEAEESVERKPLKDPSVADEMEDMNEESYIDIKDQTNQLLVEHLAEEAMEADCGPEDNKENLSTSASSTAADGLDIQLAIKEDDDEEKPLAVIADEQKPGLLLTNDMKVDEKPNGKQESVCDEHVQLVPNLRQEQEIHLQNLGLLTHQAAEHRRKCLLEAQARQAQMQLQQHHHHQHKRQGARGGGSATHVESSGTLKTVIKLNRSSNGGVSGSGGLPTGTVIHGGCGSSSASSTSSSSVGSATRKSSGTLGSGAGAGAGVRRQSLKMTFQKGRARGHGAADRSADQYGAHAEDSYYTIQNENEGAKKFVVTTGNTGRKTNNRFSSTNNYHSTVALHGSNSALQYYSSHSESQGQTDHGFYQMVKKDEKEKILIPEKASSFKFHPGRLCEDQCYYCSGKFGLYDTPCHVGQIKSVERQQKILANEEKLTVDNCLCDACFRHVDRRANVPSYKKRLSASGHLEMGSAAGSALEKQFAGDSGVITESGGEAGSTAAVAVQQRSCGVKDCVEAARHSLRRKCIRKRVKKYQLSLEIPAGSSNVGLCEAHYNTVIQFSGCVLCKRRLGKNHMYNITTQDTIRLEKALSEMGIPVQLGMGTAVCKLCRYFANLLIKPPDSTKAQKAEFVKNYRKRLLKVHNLQDGSHELSEADEEEAPNATETERPTSDGHEDPEMPMVADYDGPTDSNSSSSSTAALDTSKQMSKLQAILQQNVGADAAGAAGTGTVAASPGGSGSGADISNVLRGNPNISMRELFHGEEELGVQFKVPFGCSSSQRTPEGWTRVQTFLQYDEPTRRLWEELQKPYGNQSSFLRHLILLEKYYRNGDLVLAPHASSNATVYTETVRQRLNSFDHGHCGGLNIAGSPSSSGSGKRSGVPQPTGASVLATALTTPLTSHSSSSASISSEQHSSVDPVIPLVDLNDDDEGEDGAGGAGERESTNRQQDVILECLRTASVDKLTKQLSSNAVTIIARPKDKSQLSCNSGSSTSISSSSSAISSPEEVAVTKVTAVAPVQSKDAPPLAPASSGVSNSRSILKTNLLGMNKAVELVPLTTAPHAYKPTGCHKPEKQQKILDVANKQPGSQGEPVPSSALLGLQSKLKPPTHQQQVSGSGAGTSGSQKPSNVAQLLSSPPELISLHRRQTSGAAAGSSSFLQGKRLQLPRSGAGPSGAGTGTGAGAAGSRSAGGPPPPNVVILPDALTPQERHESKSWKPTLIPLEDQHKVPNKSHALYQTADGRRLPALVQVQSGGKPYLISIFDYNRMCILRREKLMRDQLLKSNAKPKPQNQQQQQGQTHQQQQNSAASAAAFSNMVKLAQQHTARQQLQQLQQKPQQQQQLPTLQPGGVRLARLPQKLLMPPLTNPQIGSQAPNLQPLLSSTLDNSNNCWLWKNFPDPNQYLLNGNGGGAGSSSSKLPHLTAKPATATSSSGAANKSAGSLFTLKQQQHQQKLIDNAIMSKIPKSLTVIPQQMGGNTGGDMGGSSSSGKD

Target ORF information:

RefSeq Version	NM_001258556.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258556.2

ORF Insert Sequence:

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG GTGCGAAAAA GTTTGTTGTA ACTACTGGTA ATACCGGCCG CAAGACTAAT 
AACCGTTTCA GCTCAACTAA CAACTACCAC TCGACGGTAG CCTTGCACGG TAGCAACTCT 
GCGCTCCAGT ACTATTCGTC CCACTCGGAA AGTCAGGGAC AGACGGACCA CGGCTTCTAT 
CAGATGGTCA AAAAGGACGA AAAGGAGAAG ATCCTCATTC CGGAAAAGGC CTCCTCGTTT 
AAGTTTCACC CAGGGAGACT GTGCGAAGAC CAGTGCTACT ACTGTAGCGG AAAGTTTGGC 
CTCTATGACA CCCCCTGCCA TGTTGGACAA ATAAAGTCCG TGGAGCGCCA GCAGAAGATC 
CTAGCCAACG AGGAGAAGCT CACCGTGGAT AACTGCTTGT GCGACGCATG TTTTCGACAC 
GTGGACCGCC GGGCAAATGT GCCATCCTAT AAGAAGCGTC TTTCCGCTTC AGGTCACTTG 
GAGATGGGGT CTGCAGCGGG ATCTGCACTA GAGAAACAGT TTGCTGGCGA CAGCGGCGTC 
ATTACGGAAT CGGGTGGCGA AGCTGGTTCT ACGGCAGCTG TGGCCGTGCA GCAACGATCT 
TGTGGCGTGA AGGACTGCGT CGAAGCGGCA CGACACTCGC TGCGGCGCAA GTGCATACGC 
AAGAGAGTAA AGAAGTATCA GCTCAGCCTG GAGATTCCCG CAGGCTCGTC GAACGTGGGG 
CTGTGTGAGG CACATTACAA TACGGTCATC CAATTTTCCG GCTGCGTTCT TTGCAAGCGT 
AGATTAGGCA AGAACCATAT GTACAACATA ACCACGCAGG ACACAATTCG ACTGGAAAAG 
GCGCTGTCCG AGATGGGCAT CCCAGTTCAG CTTGGCATGG GCACTGCAGT CTGCAAGCTG 
TGTCGCTATT TTGCCAACCT TTTGATAAAG CCACCGGATA GCACCAAGGC ACAAAAGGCG 
GAATTCGTGA AGAACTACAG AAAGAGGCTC CTCAAGGTGC ATAATCTGCA GGATGGCAGT 
CATGAGCTGT CCGAAGCGGA TGAAGAAGAG GCACCTAATG CAACGGAGAC AGAAAGGCCA 
ACCTCAGACG GACACGAAGA TCCCGAGATG CCCATGGTAG CGGACTATGA TGGACCTACC 
GACTCCAATT CCAGTAGTTC TTCGACTGCA GCCCTGGACA CCAGCAAACA AATGTCCAAG 
CTTCAGGCCA TCCTGCAGCA AAATGTGGGA GCGGATGCGG CAGGAGCTGC GGGAACAGGA 
ACTGTTGCAG CAAGTCCCGG AGGAAGCGGA TCTGGGGCAG ATATCTCTAA CGTATTGCGA 
GGGAATCCGA ACATTTCCAT GCGCGAACTT TTCCACGGCG AGGAAGAGCT GGGTGTGCAG 
TTCAAGGTGC CGTTCGGATG CAGCAGCAGC CAGCGTACTC CGGAGGGCTG GACACGAGTG 
CAGACTTTCC TACAATACGA TGAGCCGACG CGCCGCCTCT GGGAGGAGTT GCAAAAGCCG 
TACGGAAATC AGAGCTCATT TCTGCGCCAC TTGATACTAT TAGAGAAGTA CTACCGAAAC 
GGAGATCTCG TCCTAGCACC GCATGCTTCC TCCAATGCCA CGGTTTACAC AGAGACTGTT 
CGTCAGCGGC TGAATTCGTT TGATCACGGT CACTGCGGTG GATTGAACAT CGCAGGCAGC 
CCTTCTTCTT CGGGTTCCGG CAAGCGCAGT GGAGTTCCTC AACCTACGGG TGCCAGTGTG 
CTGGCCACCG CCCTCACAAC ACCCTTGACA AGCCATTCAT CCTCCTCTGC ATCCATTTCC 
TCCGAACAGC ATTCGTCGGT TGATCCTGTC ATTCCGCTGG TAGACCTCAA TGATGACGAT 
GAAGGCGAAG ATGGGGCAGG AGGAGCGGGC GAAAGGGAGT CGACAAATAG GCAGCAGGAC 
GTAATCTTGG AATGCCTTAG AACTGCCTCT GTGGACAAGC TGACTAAGCA GCTCAGCTCG 
AATGCGGTGA CGATTATCGC CCGGCCCAAA GACAAATCGC AGCTCTCCTG CAACAGCGGA 
TCCTCCACGT CCATTTCCAG CTCCTCGTCC GCTATTTCCT CGCCGGAGGA AGTGGCCGTC 
ACTAAGGTTA CAGCAGTCGC ACCAGTCCAG TCCAAGGATG CACCGCCACT GGCGCCAGCA 
AGTAGCGGTG TTAGCAACAG TCGTAGTATC CTTAAAACCA ACCTCTTGGG CATGAACAAG 
GCCGTGGAAC TCGTGCCCTT AACGACTGCC CCCCACGCTT ACAAGCCAAC TGGATGCCAT 
AAGCCTGAGA AACAGCAAAA GATTCTTGAC GTGGCCAATA AGCAGCCCGG TAGCCAGGGG 
GAACCGGTAC CATCAAGCGC CTTGCTTGGC CTGCAGTCAA AGCTAAAGCC TCCAACGCAT 
CAGCAGCAGG TCAGCGGATC AGGAGCGGGA ACTAGTGGTT CTCAGAAGCC ATCTAATGTG 
GCGCAATTGC TTAGCTCTCC ACCGGAGCTA ATCAGCTTGC ATCGACGGCA GACCAGCGGA 
GCAGCAGCGG GGTCCAGCAG CTTCCTTCAG GGCAAGAGGC TTCAACTTCC ACGATCTGGA 
GCAGGGCCTT CAGGAGCGGG AACGGGAACA GGCGCTGGAG CAGCAGGAAG CCGCAGTGCG 
GGTGGACCAC CACCGCCCAA TGTGGTCATA CTGCCGGACG CCTTAACCCC CCAGGAGCGA 
CACGAGAGCA AGAGCTGGAA GCCAACGCTG ATACCGCTGG AGGATCAGCA CAAGGTGCCG 
AACAAATCAC ATGCTCTTTA TCAGACCGCC GACGGTCGAA GGTTGCCCGC CCTGGTGCAA 
GTGCAGTCTG GTGGCAAGCC ATACCTCATC TCTATCTTCG ACTATAACCG CATGTGCATC 
TTGCGAAGGG AAAAGCTGAT GCGGGACCAG TTGCTCAAGA GTAACGCCAA GCCAAAGCCG 
CAGAACCAGC AACAGCAGCA GGGCCAAACG CACCAGCAGC AGCAGAATTC CGCCGCATCG 
GCGGCTGCCT TCTCCAACAT GGTGAAGTTG GCCCAGCAAC ACACGGCGCG ACAGCAGCTT 
CAGCAGCTGC AACAGAAGCC ACAACAGCAG CAACAATTGC CCACTTTGCA GCCAGGTGGG 
GTGCGACTTG CCCGGCTGCC GCAAAAACTA CTGATGCCAC CACTGACTAA TCCGCAGATT 
GGCAGTCAAG CACCCAACTT ACAGCCGTTG CTGTCTAGTA CGCTGGATAA CAGCAACAAC 
TGCTGGCTGT GGAAAAACTT TCCTGATCCC AATCAGTATC TGCTAAATGG AAACGGAGGG 
GGTGCCGGGA GCTCCTCCAG CAAGTTGCCA CATCTCACGG CCAAACCAGC CACGGCAACT 
AGTAGCTCCG GAGCGGCCAA CAAATCAGCA GGAAGCCTAT TTACCCTCAA GCAGCAGCAG 
CACCAGCAGA AACTCATCGA CAACGCTATC ATGTCAAAGA TACCCAAAAG TCTGACAGTA 
ATACCGCAGC AGATGGGTGG TAATACCGGT GGCGATATGG GGGGCAGCAG CTCCTCCGGC 
AAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

east ( NM_001297869.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284798.1 Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant G, mRNA.

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CloneID	OFa22257
Clone ID Related Accession (Same CDS sequence)	NM_001297869.1
Accession Version	NM_001297869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhanced adult sensory threshold, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCATTTCCT TATCATTTGC AGACGAGGAG AAGCTCACCG TGGATAACTG CTTGTGCGAC 
GCATGTTTTC GACACGTGGA CCGCCGGGCA AATGTGCCAT CCTATAAGAA GCGTCTTTCC 
GCTTCAGGTC ACTTGGAGAT GGGGTCTGCA GCGGGATCTG CACTAGAGAA ACAGTTTGCT 
GGCGACAGCG GCGTCATTAC GGAATCGGGT GGCGAAGCTG GTTCTACGGC AGCTGTGGCC 
GTGCAGCAAC GATCTTGTGG CGTGAAGGAC TGCGTCGAAG CGGCACGACA CTCGCTGCGG 
CGCAAGTGCA TACGCAAGAG AGTAAAGAAG TATCAGCTCA GCCTGGAGAT TCCCGCAGGC 
TCGTCGAACG TGGGGCTGTG TGAGGCACAT TACAATACGG TCATCCAATT TTCCGGCTGC 
GTTCTTTGCA AGCGTAGATT AGGCAAGAAC CATATGTACA ACATAACCAC GGACACAATT 
CGACTGGAAA AGGCGCTGTC CGAGATGGGC ATCCCAGTTC AGCTTGGCAT GGGCACTGCA 
GTCTGCAAGC TGTGTCGCTA TTTTGCCAAC CTTTTGATAA AGCCACCGGA TAGCACCAAG 
GCACAAAAGG CGGAATTCGT GAAGAACTAC AGAAAGAGGC TCCTCAAGGT GCATAATCTG 
CAGGATGGCA GTCATGAGCT GTCCGAAGCG GATGAAGAAG AGGCACCTAA TGCAACGGAG 
ACAGAAAGGC CAACCTCAGA CGGACACGAA GATCCCGAGA TGCCCATGGT AGCGGACTAT 
GATGGACCTA CCGACTCCAA TTCCAGTAGT TCTTCGACTG CAGCCCTGGA CACCAGCAAA 
CAAATGTCCA AGCTTCAGGC CATCCTGCAG CAAAATGTGG GAGCGGATGC GGCAGGAGCT 
GCGGGAACAG GAACTGTTGC AGCAAGTCCC GGAGGAAGCG GATCTGGGGC AGATATCTCT 
AACGTATTGC GAGGGAATCC GAACATTTCC ATGCGCGAAC TTTTCCACGG CGAGGAAGAG 
CTGGGTGTGC AGTTCAAGGT GCCGTTCGGA TGCAGCAGCA GCCAGCGTAC TCCGGAGGGC 
TGGACACGAG TGCAGACTTT CCTACAATAC GATGAGCCGA CGCGCCGCCT CTGGGAGGAG 
TTGCAAAAGC CGTACGGAAA TCAGAGCTCA TTTCTGCGCC ACTTGATACT ATTAGAGAAG 
TACTACCGAA ACGGAGATCT CGTCCTAGCA CCGCATGCTT CCTCCAATGC CACGGTTTAC 
ACAGAGACTG TTCGTCAGCG GCTGAATTCG TTTGATCACG GTCACTGCGG TGGATTGAAC 
ATCGCAGGCA GCCCTTCTTC TTCGGGTTCC GGCAAGCGCA GTGGAGTTCC TCAACCTACG 
GGTGCCAGTG TGCTGGCCAC CGCCCTCACA ACACCCTTGA CAAGCCATTC ATCCTCCTCT 
GCATCCATTT CCTCCGAACA GCATTCGTCG GTTGATCCTG TCATTCCGCT GGTAGACCTC 
AATGATGACG ATGAAGGCGA AGATGGGGCA GGAGGAGCGG GCGAAAGGGA GTCGACAAAT 
AGGCAGCAGG ACGTAATCTT GGAATGCCTT AGAACTGCCT CTGTGGACAA GCTGACTAAG 
CAGCTCAGCT CGAATGCGGT GACGATTATC GCCCGGCCCA AAGACAAATC GCAGCTCTCC 
TGCAACAGCG GATCCTCCAC GTCCATTTCC AGCTCCTCGT CCGCTATTTC CTCGCCGGAG 
GAAGTGGCCG TCACTAAGGT TACAGCAGTC GCACCAGTCC AGTCCAAGGA TGCACCGCCA 
CTGGCGCCAG CAAGTAGCGG TGTTAGCAAC AGTCGTAGTA TCCTTAAAAC CAACCTCTTG 
GGCATGAACA AGGCCGTGGA ACTCGTGCCC TTAACGACTG CCCCCCACGC TTACAAGCCA 
ACTGGATGCC ATAAGCCTGA GAAACAGCAA AAGATTCTTG ACGTGGCCAA TAAGCAGCCC 
GGTAGCCAGG GGGAACCGGT ACCATCAAGC GCCTTGCTTG GCCTGCAGTC AAAGCTAAAG 
CCTCCAACGC ATCAGCAGCA GGTCAGCGGA TCAGGAGCGG GAACTAGTGG TTCTCAGAAG 
CCATCTAATG TGGCGCAATT GCTTAGCTCT CCACCGGAGC TAATCAGCTT GCATCGACGG 
CAGACCAGCG GAGCAGCAGC GGGGTCCAGC AGCTTCCTTC AGGGCAAGAG GCTTCAACTT 
CCACGATCTG GAGCAGGGCC TTCAGGAGCG GGAACGGGAA CAGGCGCTGG AGCAGCAGGA 
AGCCGCAGTG CGGGTGGACC ACCACCGCCC AATGTGGTCA TACTGCCGGA CGCCTTAACC 
CCCCAGGAGC GACACGAGAG CAAGAGCTGG AAGCCAACGC TGATACCGCT GGAGGATCAG 
CACAAGGTGC CGAACAAATC ACATGCTCTT TATCAGACCG CCGACGGTCG AAGGTTGCCC 
GCCCTGGTGC AAGTGCAGTC TGGTGGCAAG CCATACCTCA TCTCTATCTT CGACTATAAC 
CGCATGTGCA TCTTGCGAAG GGAAAAGCTG ATGCGGGACC AGTTGCTCAA GAGTAACGCC 
AAGCCAAAGC CGCAGAACCA GCAACAGCAG CAGGGCCAAA CGCACCAGCA GCAGCAGAAT 
TCCGCCGCAT CGGCGGCTGC CTTCTCCAAC ATGGTGAAGT TGGCCCAGCA ACACACGGCG 
CGACAGCAGC TTCAGCAGCT GCAACAGAAG CCACAACAGC AGCAACAATT GCCCACTTTG 
CAGCCAGGTG GGGTGCGACT TGCCCGGCTG CCGCAAAAAC TACTGATGCC ACCACTGACT 
AATCCGCAGA TTGGCAGTCA AGCACCCAAC TTACAGCCGT TGCTGTCTAG TACGCTGGAT 
AACAGCAACA ACTGCTGGCT GTGGAAAAAC TTTCCTGATC CCAATCAGTA TCTGCTAAAT 
GGAAACGGAG GGGGTGCCGG GAGCTCCTCC AGCAAGTTGC CACATCTCAC GGCCAAACCA 
GCCACGGCAA CTAGTAGCTC CGGAGCGGCC AACAAATCAG CAGGAAGCCT ATTTACCCTC 
AAGCAGCAGC AGCACCAGCA GAAACTCATC GACAACGCTA TCATGTCAAA GATACCCAAA 
AGTCTGACAG TAATACCGCA GCAGATGGGT GGTAATACCG GTGGCGATAT GGGGGGCAGC 
AGCTCCTCCG GCAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284798.1
CDS	846..7766
Translation	MSSRKVPGGSGGADESTAAAAPLDDNANASVEIPDSSEEPAMGVGEEMSIISKTRTSTLSVEPAKEPTVTAELEGEKELESNPVSKTPRSTPTPTLTPAVTPTASDGVAAKSVRVTRHSSPLLLIISPTTSRREVGDGELDTEEPTGSGGQRKSSVERSLAPVIRGRKSIKDLKEAKEVKSEEPPAAASESRAASGVTPGQVKEQHVADGNEMESLPITDKKDHKDTKDKGDERETDQEEEKEKSADTEIIADTEKTSEKQKYTEKDKAADKDGGKEKDIDANKDIDKEKEKVKEVLPPVVPIAPVTPTCNRVTRKSHAQEQAINTRVTRNRRQSSTVGANSTASLVAASSSVTEQPPPSRGRRKKPVVVAPPLEPAVKRKRSQDVEADSDANNSTKYSKVEVVKSEEAEAPEEDSSAVPIKQESVDGNEVSSISPTVTPTPTPAPTPAPVPGSRRGRGRPQNRNSSSPATTTRATRLSKAGSPVILTPVAQEPAPPKRRRVGSSTRKTVSASSLAPSSQGGAGDEDSKDSMASSMDDLLMAAADIKQEKLTPDFDDSLMPEGLPSTSGASSANGHSCTEPLTVDTEINVKPADSKVKPKESPVVAVEESPSQSETQSAKVSAHAGKAPSLSPDMISEGVSAVSVRKFYKKPEFLENNLGIEKDPELGEIVQTVSNNDTETDVEMAVDGEVNQPSTPKSQDKKKEEQEKNQKSGLKAAKKAPAKLEPKAEDISEILTDVPVDISTEAVEIIEEAEEDTCSNSSIKPGELRLDESNDEPELLLEDALIVNGDENETPDQPEEKEDQVEFFHTGEYDDFEHEIMVELAKEGVLDASGNALSQQKVELEHPEDVTLHESKNDIEAEESVERKPLKDPSVADEMEDMNEESYIDIKDQTNQLLVEHLAEEAMEADCGPEDNKENLSTSASSTAADGLDIQLAIKEDDDEEKPLAVIADEQKPGLLLTNDMKVDEKPNGKQESVCDEHVQLVPNLRQEQEIHLQNLGLLTHQAAEHRRKCLLEAQARQAQMQLQQHHHHQHKRQGARGGGSATHVESSGTLKTVIKLNRSSNGGVSGSGGLPTGTVIHGGCGSSSASSTSSSSVGSATRKSSGTLGSGAGAGAGVRRQSLKMTFQKGRARGHGAADRSADQYGAHAEDSYYTIQNENEDSSHSESQGQTDHGFYQMVKKDEKEKILIPEKASSFKFHPGRLCEDQCYYCSGKFGLYDTPCHVGQIKSVERQQKILAISLSFADEEKLTVDNCLCDACFRHVDRRANVPSYKKRLSASGHLEMGSAAGSALEKQFAGDSGVITESGGEAGSTAAVAVQQRSCGVKDCVEAARHSLRRKCIRKRVKKYQLSLEIPAGSSNVGLCEAHYNTVIQFSGCVLCKRRLGKNHMYNITTDTIRLEKALSEMGIPVQLGMGTAVCKLCRYFANLLIKPPDSTKAQKAEFVKNYRKRLLKVHNLQDGSHELSEADEEEAPNATETERPTSDGHEDPEMPMVADYDGPTDSNSSSSSTAALDTSKQMSKLQAILQQNVGADAAGAAGTGTVAASPGGSGSGADISNVLRGNPNISMRELFHGEEELGVQFKVPFGCSSSQRTPEGWTRVQTFLQYDEPTRRLWEELQKPYGNQSSFLRHLILLEKYYRNGDLVLAPHASSNATVYTETVRQRLNSFDHGHCGGLNIAGSPSSSGSGKRSGVPQPTGASVLATALTTPLTSHSSSSASISSEQHSSVDPVIPLVDLNDDDEGEDGAGGAGERESTNRQQDVILECLRTASVDKLTKQLSSNAVTIIARPKDKSQLSCNSGSSTSISSSSSAISSPEEVAVTKVTAVAPVQSKDAPPLAPASSGVSNSRSILKTNLLGMNKAVELVPLTTAPHAYKPTGCHKPEKQQKILDVANKQPGSQGEPVPSSALLGLQSKLKPPTHQQQVSGSGAGTSGSQKPSNVAQLLSSPPELISLHRRQTSGAAAGSSSFLQGKRLQLPRSGAGPSGAGTGTGAGAAGSRSAGGPPPPNVVILPDALTPQERHESKSWKPTLIPLEDQHKVPNKSHALYQTADGRRLPALVQVQSGGKPYLISIFDYNRMCILRREKLMRDQLLKSNAKPKPQNQQQQQGQTHQQQQNSAASAAAFSNMVKLAQQHTARQQLQQLQQKPQQQQQLPTLQPGGVRLARLPQKLLMPPLTNPQIGSQAPNLQPLLSSTLDNSNNCWLWKNFPDPNQYLLNGNGGGAGSSSSKLPHLTAKPATATSSSGAANKSAGSLFTLKQQQHQQKLIDNAIMSKIPKSLTVIPQQMGGNTGGDMGGSSSSGKD

Target ORF information:

RefSeq Version	NM_001297869.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhanced adult sensory threshold (east), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297869.1

ORF Insert Sequence:

ATGTCTAGCC GGAAGGTGCC AGGAGGCTCT GGAGGAGCTG ACGAATCCAC AGCAGCAGCT 
GCCCCCCTGG ATGATAATGC CAATGCCAGT GTGGAGATTC CAGACAGCAG CGAGGAGCCA 
GCAATGGGCG TCGGCGAAGA GATGTCTATC ATAAGCAAAA CACGCACCTC AACTTTGTCA 
GTGGAGCCCG CTAAGGAGCC AACAGTAACA GCAGAGCTGG AAGGCGAAAA AGAGCTGGAA 
TCGAATCCAG TCTCCAAAAC TCCTAGGTCC ACGCCTACGC CAACCCTTAC GCCAGCCGTC 
ACGCCTACCG CCAGTGATGG AGTGGCGGCC AAGAGCGTGA GGGTTACCCG GCACTCGTCG 
CCACTGCTTC TGATCATCTC GCCCACGACA AGTAGACGTG AGGTCGGCGA CGGAGAGCTA 
GACACCGAGG AACCAACGGG ATCGGGTGGC CAAAGAAAGA GCTCCGTGGA GCGATCTTTG 
GCGCCCGTTA TACGCGGACG AAAGTCCATC AAGGATCTGA AAGAAGCCAA AGAAGTCAAG 
TCCGAGGAGC CGCCTGCCGC AGCATCAGAG TCACGAGCTG CAAGTGGAGT GACGCCTGGC 
CAGGTCAAGG AACAGCATGT CGCGGATGGC AACGAAATGG AATCCTTGCC AATCACAGAC 
AAGAAAGACC ACAAAGACAC AAAAGACAAG GGAGATGAGC GGGAAACCGA TCAGGAGGAA 
GAGAAGGAAA AATCAGCTGA TACAGAAATA ATTGCAGATA CAGAAAAAAC TTCGGAGAAA 
CAAAAGTATA CAGAGAAGGA CAAAGCTGCC GATAAAGATG GAGGAAAAGA AAAAGATATT 
GATGCAAATA AGGATATAGA TAAGGAGAAG GAAAAGGTCA AGGAAGTACT TCCGCCAGTG 
GTGCCTATAG CACCAGTGAC ACCCACTTGT AACCGTGTCA CACGTAAATC ACATGCCCAG 
GAGCAGGCGA TTAACACGCG GGTCACTCGC AATCGTCGCC AGTCCTCTAC AGTTGGAGCC 
AACTCCACCG CGTCTTTGGT AGCTGCATCC TCCTCAGTAA CAGAGCAACC CCCTCCATCT 
CGCGGTCGAC GGAAGAAGCC AGTGGTGGTG GCTCCTCCCT TGGAGCCTGC GGTAAAACGG 
AAGCGATCGC AAGATGTTGA AGCCGACTCA GACGCCAACA ACAGCACGAA ATACAGCAAG 
GTGGAAGTGG TAAAGTCTGA GGAAGCTGAG GCACCAGAGG AGGACTCCAG TGCCGTGCCC 
ATTAAGCAGG AATCTGTTGA TGGCAACGAG GTCAGTTCTA TTTCTCCAAC AGTCACGCCC 
ACACCCACAC CTGCGCCAAC ACCAGCTCCA GTCCCGGGCA GTCGACGGGG TCGTGGGCGC 
CCGCAGAACA GGAACTCCTC TTCGCCTGCA ACCACAACGC GGGCAACGCG GCTAAGCAAG 
GCGGGATCAC CGGTTATCCT GACGCCAGTA GCCCAGGAAC CGGCGCCACC GAAACGGCGG 
CGAGTCGGCT CCAGCACACG GAAGACTGTC TCGGCCAGCT CGCTGGCACC CAGCTCGCAG 
GGCGGCGCCG GGGATGAGGA CTCCAAGGAC AGTATGGCCT CGTCCATGGA CGACCTGCTG 
ATGGCCGCAG CAGATATCAA GCAGGAGAAG CTGACGCCCG ATTTCGACGA TAGTTTGATG 
CCAGAAGGCC TGCCCTCTAC TTCTGGTGCG TCGAGTGCCA ATGGTCATTC CTGCACCGAA 
CCGCTTACTG TGGACACGGA AATTAATGTT AAGCCCGCTG ATTCCAAAGT AAAACCAAAG 
GAGTCACCGG TGGTAGCAGT CGAGGAATCT CCATCACAAT CCGAAACGCA ATCTGCAAAG 
GTGTCAGCGC ATGCGGGGAA GGCTCCATCT CTTAGTCCAG ATATGATAAG TGAAGGCGTG 
AGCGCGGTCA GTGTTCGAAA GTTTTATAAG AAGCCTGAGT TCCTGGAAAA CAATCTGGGC 
ATTGAAAAGG ATCCGGAGCT AGGTGAAATC GTTCAGACGG TTAGTAACAA TGACACGGAA 
ACAGATGTGG AGATGGCTGT TGATGGCGAG GTGAATCAAC CGTCAACTCC CAAGTCGCAG 
GATAAAAAGA AAGAGGAGCA GGAAAAGAAT CAGAAATCAG GGCTAAAGGC AGCAAAGAAG 
GCTCCTGCTA AGTTAGAACC TAAAGCTGAA GACATTTCTG AAATTCTTAC TGACGTTCCT 
GTTGATATTT CGACTGAGGC AGTAGAAATT ATAGAAGAAG CAGAGGAAGA CACTTGTTCA 
AATAGCTCAA TCAAACCAGG TGAGCTCCGA CTGGACGAGA GCAACGATGA ACCTGAACTG 
CTTCTTGAAG ACGCCCTCAT AGTCAATGGT GATGAGAATG AGACACCAGA TCAACCGGAG 
GAAAAGGAGG ACCAGGTGGA GTTCTTCCAT ACAGGAGAAT ACGACGACTT TGAGCACGAG 
ATTATGGTGG AGCTGGCGAA GGAGGGGGTG CTAGATGCCA GCGGCAATGC ATTAAGTCAG 
CAAAAGGTAG AACTTGAGCA TCCCGAGGAT GTAACTCTAC ACGAATCAAA AAATGACATA 
GAAGCCGAAG AATCGGTTGA ACGTAAGCCT CTTAAGGACC CGTCGGTTGC GGACGAAATG 
GAGGACATGA ATGAGGAATC CTATATTGAC ATTAAGGACC AGACAAATCA ACTGTTAGTT 
GAACACTTGG CAGAAGAGGC CATGGAAGCG GACTGCGGTC CCGAGGATAA CAAGGAGAAC 
TTGTCCACGT CTGCTTCGAG CACCGCTGCC GATGGTCTAG ATATTCAGTT GGCCATCAAG 
GAGGATGACG ACGAGGAGAA ACCGCTTGCA GTTATCGCTG ACGAACAGAA GCCTGGGCTG 
CTGTTGACCA ATGACATGAA AGTGGATGAG AAACCAAATG GCAAGCAGGA ATCGGTCTGT 
GATGAGCACG TTCAGCTGGT GCCAAACCTT CGTCAAGAAC AGGAAATTCA CTTACAAAAT 
CTGGGCCTAC TCACGCACCA GGCCGCTGAA CATAGGCGCA AGTGTCTGCT TGAGGCACAG 
GCCCGCCAGG CGCAAATGCA GCTCCAGCAA CATCACCACC ATCAGCACAA GCGACAAGGA 
GCGCGCGGAG GAGGCAGTGC CACTCATGTG GAATCCAGCG GTACTTTGAA GACAGTCATC 
AAGCTGAACA GGAGCAGCAA CGGAGGAGTA AGCGGTAGTG GCGGCCTGCC TACTGGTACA 
GTTATCCATG GAGGCTGTGG CTCCTCTTCA GCTTCTTCCA CGTCCTCCTC CTCGGTGGGC 
AGTGCCACAC GTAAGTCAAG CGGGACCTTG GGCTCAGGAG CGGGAGCAGG AGCTGGCGTT 
CGCCGGCAGT CGCTTAAGAT GACATTCCAG AAGGGTCGGG CTCGTGGTCA CGGTGCTGCG 
GATCGATCCG CCGATCAGTA TGGCGCCCAC GCCGAGGACT CCTACTACAC CATTCAAAAC 
GAGAACGAAG ATTCGTCCCA CTCGGAAAGT CAGGGACAGA CGGACCACGG CTTCTATCAG 
ATGGTCAAAA AGGACGAAAA GGAGAAGATC CTCATTCCGG AAAAGGCCTC CTCGTTTAAG 
TTTCACCCAG GGAGACTGTG CGAAGACCAG TGCTACTACT GTAGCGGAAA GTTTGGCCTC 
TATGACACCC CCTGCCATGT TGGACAAATA AAGTCCGTGG AGCGCCAGCA GAAGATCCTA 
GCCATTTCCT TATCATTTGC AGACGAGGAG AAGCTCACCG TGGATAACTG CTTGTGCGAC 
GCATGTTTTC GACACGTGGA CCGCCGGGCA AATGTGCCAT CCTATAAGAA GCGTCTTTCC 
GCTTCAGGTC ACTTGGAGAT GGGGTCTGCA GCGGGATCTG CACTAGAGAA ACAGTTTGCT 
GGCGACAGCG GCGTCATTAC GGAATCGGGT GGCGAAGCTG GTTCTACGGC AGCTGTGGCC 
GTGCAGCAAC GATCTTGTGG CGTGAAGGAC TGCGTCGAAG CGGCACGACA CTCGCTGCGG 
CGCAAGTGCA TACGCAAGAG AGTAAAGAAG TATCAGCTCA GCCTGGAGAT TCCCGCAGGC 
TCGTCGAACG TGGGGCTGTG TGAGGCACAT TACAATACGG TCATCCAATT TTCCGGCTGC 
GTTCTTTGCA AGCGTAGATT AGGCAAGAAC CATATGTACA ACATAACCAC GGACACAATT 
CGACTGGAAA AGGCGCTGTC CGAGATGGGC ATCCCAGTTC AGCTTGGCAT GGGCACTGCA 
GTCTGCAAGC TGTGTCGCTA TTTTGCCAAC CTTTTGATAA AGCCACCGGA TAGCACCAAG 
GCACAAAAGG CGGAATTCGT GAAGAACTAC AGAAAGAGGC TCCTCAAGGT GCATAATCTG 
CAGGATGGCA GTCATGAGCT GTCCGAAGCG GATGAAGAAG AGGCACCTAA TGCAACGGAG 
ACAGAAAGGC CAACCTCAGA CGGACACGAA GATCCCGAGA TGCCCATGGT AGCGGACTAT 
GATGGACCTA CCGACTCCAA TTCCAGTAGT TCTTCGACTG CAGCCCTGGA CACCAGCAAA 
CAAATGTCCA AGCTTCAGGC CATCCTGCAG CAAAATGTGG GAGCGGATGC GGCAGGAGCT 
GCGGGAACAG GAACTGTTGC AGCAAGTCCC GGAGGAAGCG GATCTGGGGC AGATATCTCT 
AACGTATTGC GAGGGAATCC GAACATTTCC ATGCGCGAAC TTTTCCACGG CGAGGAAGAG 
CTGGGTGTGC AGTTCAAGGT GCCGTTCGGA TGCAGCAGCA GCCAGCGTAC TCCGGAGGGC 
TGGACACGAG TGCAGACTTT CCTACAATAC GATGAGCCGA CGCGCCGCCT CTGGGAGGAG 
TTGCAAAAGC CGTACGGAAA TCAGAGCTCA TTTCTGCGCC ACTTGATACT ATTAGAGAAG 
TACTACCGAA ACGGAGATCT CGTCCTAGCA CCGCATGCTT CCTCCAATGC CACGGTTTAC 
ACAGAGACTG TTCGTCAGCG GCTGAATTCG TTTGATCACG GTCACTGCGG TGGATTGAAC 
ATCGCAGGCA GCCCTTCTTC TTCGGGTTCC GGCAAGCGCA GTGGAGTTCC TCAACCTACG 
GGTGCCAGTG TGCTGGCCAC CGCCCTCACA ACACCCTTGA CAAGCCATTC ATCCTCCTCT 
GCATCCATTT CCTCCGAACA GCATTCGTCG GTTGATCCTG TCATTCCGCT GGTAGACCTC 
AATGATGACG ATGAAGGCGA AGATGGGGCA GGAGGAGCGG GCGAAAGGGA GTCGACAAAT 
AGGCAGCAGG ACGTAATCTT GGAATGCCTT AGAACTGCCT CTGTGGACAA GCTGACTAAG 
CAGCTCAGCT CGAATGCGGT GACGATTATC GCCCGGCCCA AAGACAAATC GCAGCTCTCC 
TGCAACAGCG GATCCTCCAC GTCCATTTCC AGCTCCTCGT CCGCTATTTC CTCGCCGGAG 
GAAGTGGCCG TCACTAAGGT TACAGCAGTC GCACCAGTCC AGTCCAAGGA TGCACCGCCA 
CTGGCGCCAG CAAGTAGCGG TGTTAGCAAC AGTCGTAGTA TCCTTAAAAC CAACCTCTTG 
GGCATGAACA AGGCCGTGGA ACTCGTGCCC TTAACGACTG CCCCCCACGC TTACAAGCCA 
ACTGGATGCC ATAAGCCTGA GAAACAGCAA AAGATTCTTG ACGTGGCCAA TAAGCAGCCC 
GGTAGCCAGG GGGAACCGGT ACCATCAAGC GCCTTGCTTG GCCTGCAGTC AAAGCTAAAG 
CCTCCAACGC ATCAGCAGCA GGTCAGCGGA TCAGGAGCGG GAACTAGTGG TTCTCAGAAG 
CCATCTAATG TGGCGCAATT GCTTAGCTCT CCACCGGAGC TAATCAGCTT GCATCGACGG 
CAGACCAGCG GAGCAGCAGC GGGGTCCAGC AGCTTCCTTC AGGGCAAGAG GCTTCAACTT 
CCACGATCTG GAGCAGGGCC TTCAGGAGCG GGAACGGGAA CAGGCGCTGG AGCAGCAGGA 
AGCCGCAGTG CGGGTGGACC ACCACCGCCC AATGTGGTCA TACTGCCGGA CGCCTTAACC 
CCCCAGGAGC GACACGAGAG CAAGAGCTGG AAGCCAACGC TGATACCGCT GGAGGATCAG 
CACAAGGTGC CGAACAAATC ACATGCTCTT TATCAGACCG CCGACGGTCG AAGGTTGCCC 
GCCCTGGTGC AAGTGCAGTC TGGTGGCAAG CCATACCTCA TCTCTATCTT CGACTATAAC 
CGCATGTGCA TCTTGCGAAG GGAAAAGCTG ATGCGGGACC AGTTGCTCAA GAGTAACGCC 
AAGCCAAAGC CGCAGAACCA GCAACAGCAG CAGGGCCAAA CGCACCAGCA GCAGCAGAAT 
TCCGCCGCAT CGGCGGCTGC CTTCTCCAAC ATGGTGAAGT TGGCCCAGCA ACACACGGCG 
CGACAGCAGC TTCAGCAGCT GCAACAGAAG CCACAACAGC AGCAACAATT GCCCACTTTG 
CAGCCAGGTG GGGTGCGACT TGCCCGGCTG CCGCAAAAAC TACTGATGCC ACCACTGACT 
AATCCGCAGA TTGGCAGTCA AGCACCCAAC TTACAGCCGT TGCTGTCTAG TACGCTGGAT 
AACAGCAACA ACTGCTGGCT GTGGAAAAAC TTTCCTGATC CCAATCAGTA TCTGCTAAAT 
GGAAACGGAG GGGGTGCCGG GAGCTCCTCC AGCAAGTTGC CACATCTCAC GGCCAAACCA 
GCCACGGCAA CTAGTAGCTC CGGAGCGGCC AACAAATCAG CAGGAAGCCT ATTTACCCTC 
AAGCAGCAGC AGCACCAGCA GAAACTCATC GACAACGCTA TCATGTCAAA GATACCCAAA 
AGTCTGACAG TAATACCGCA GCAGATGGGT GGTAATACCG GTGGCGATAT GGGGGGCAGC 
AGCTCCTCCG GCAAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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east cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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