Treh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Treh
Entrez Gene ID	45368
Full Name	Trehalase
Synonyms	87D3T,CG9364,Dmel\CG9364,ESTS:87D3T,TRE,TREH,Tre,anon-EST:Posey159,anon-sts38
General protein information	Preferred Names Trehalase Names CG9364 gene product from transcript CG9364-RE CG9364-PA CG9364-PB CG9364-PC CG9364-PD CG9364-PE CG9364-PF CG9364-PG Treh-PA Treh-PB Treh-PC Treh-PD Treh-PE Treh-PF Treh-PG trehalase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 57B16-57B19

mRNA and Protein(s)

mRNA	Protein	Name
NM_166423.3	NP_726025.1	trehalase, isoform C
NM_166422.2	NP_726024.1	trehalase, isoform E
NM_166421.2	NP_726023.1	trehalase, isoform A
NM_001274186.1	NP_001261115.1	trehalase, isoform G
NM_080082.3	NP_524821.1	trehalase, isoform D
NM_166424.2	NP_726026.1	trehalase, isoform F
NM_166425.2	NP_726027.1	trehalase, isoform B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Treh	NP_067456.1
Drosophila melanogaster (fruit fly)	Treh	NP_726026.1
Xenopus tropicalis (tropical clawed frog)	treh	XP_002935060.2
Pan troglodytes (chimpanzee)	TREH	XP_522200.2
Bos taurus (cattle)	TREH	NP_001179499.1
Danio rerio (zebrafish)	treh	XP_001336187.3
Neurospora crassa	NCU00943	XP_965136.1
Rattus norvegicus (Norway rat)	Treh	NP_001129613.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012053	XP_320471.4
Oryza sativa (rice)	Os10g0521000	NP_001065087.1
Homo sapiens (human)	TREH	NP_009111.2
Canis lupus familiaris (dog)	TREH	XP_005619766.1
Caenorhabditis elegans (roundworm)	tre-1	NP_491890.2
Caenorhabditis elegans (roundworm)	tre-3	NP_001256361.1
Magnaporthe oryzae (rice blast fungus)	MGG_01261	XP_003714173.1
Caenorhabditis elegans (roundworm)	tre-5	NP_493649.2
Arabidopsis thaliana (thale cress)	TRE1	NP_194135.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The present study holds significance in understanding the structural aspects of Drosophila trehalase in spite of unavailabilty of eukaryotic trehalase crystal structure.
Title: Molecular cloning and in silico studies of physiologically significant trehalase from Drosophila melanogaster.

Treh plays a role in neuroepithelial stem cell maintenance and differentiation during Drosophila optic lobe development.
Title: Trehalase regulates neuroepithelial stem cell maintenance and differentiation in the Drosophila optic lobe.

The following Treh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Treh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11400	NM_080082.3 Latest version!	Drosophila melanogaster trehalase, transcript variant D (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11400	NM_001274186.1 Latest version!	Drosophila melanogaster trehalase, transcript variant G (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11400	NM_166422.2 Latest version!	Drosophila melanogaster trehalase, transcript variant E (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11400	NM_166421.2 Latest version!	Drosophila melanogaster trehalase, transcript variant A (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11401	NM_166424.2 Latest version!	Drosophila melanogaster trehalase, transcript variant F (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11401	NM_166423.3 Latest version!	Drosophila melanogaster trehalase, transcript variant C (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11402	NM_166425.2 Latest version!	Drosophila melanogaster trehalase, transcript variant B (Treh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Treh ( NM_166422.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726024.1 Drosophila melanogaster trehalase, transcript variant E (Treh), mRNA.

Price & Availability↑

CloneID	OFa11400
Clone ID Related Accession (Same CDS sequence)	NM_166422.2 , NM_166421.2 , NM_001274186.1 , NM_080082.3
Accession Version	NM_166422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166422.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726024.1
CDS	533..2215
Translation	MASPANPSSNHKMNGNGKIYCEGNLLHTIQTAVPKLFADSKTFVDMKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_166422.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant E (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166422.2

ORF Insert Sequence:

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Treh ( NM_166421.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726023.1 Drosophila melanogaster trehalase, transcript variant A (Treh), mRNA.

Price & Availability↑

CloneID	OFa11400
Clone ID Related Accession (Same CDS sequence)	NM_166422.2 , NM_166421.2 , NM_001274186.1 , NM_080082.3
Accession Version	NM_166421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166421.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726023.1
CDS	251..1933
Translation	MASPANPSSNHKMNGNGKIYCEGNLLHTIQTAVPKLFADSKTFVDMKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_166421.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant A (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166421.2

ORF Insert Sequence:

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Treh ( NM_001274186.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261115.1 Drosophila melanogaster trehalase, transcript variant G (Treh), mRNA.

Price & Availability↑

CloneID	OFa11400
Clone ID Related Accession (Same CDS sequence)	NM_166422.2 , NM_166421.2 , NM_001274186.1 , NM_080082.3
Accession Version	NM_001274186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261115.1
CDS	484..2166
Translation	MASPANPSSNHKMNGNGKIYCEGNLLHTIQTAVPKLFADSKTFVDMKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_001274186.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant G (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274186.1

ORF Insert Sequence:

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Treh ( NM_080082.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524821.1 Drosophila melanogaster trehalase, transcript variant D (Treh), mRNA.

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CloneID	OFa11400
Clone ID Related Accession (Same CDS sequence)	NM_166422.2 , NM_166421.2 , NM_001274186.1 , NM_080082.3
Accession Version	NM_080082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_080082.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524821.1
CDS	485..2167
Translation	MASPANPSSNHKMNGNGKIYCEGNLLHTIQTAVPKLFADSKTFVDMKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_080082.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant D (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080082.3

ORF Insert Sequence:

ATGGCCTCTC CAGCGAATCC ATCGAGCAAT CACAAAATGA ACGGAAATGG TAAAATCTAC 
TGCGAGGGCA ATCTGCTGCA CACCATCCAA ACGGCAGTGC CCAAACTATT TGCGGATTCG 
AAAACGTTTG TGGACATGAA GCTGAACAAT TCGCCCGACA AGACCCTCGA GGACTTTAAT 
GCCATGATGG AGGCCAAGAA TCAGACGCCA AGCAGTGAGG ATCTCAAGCA GTTTGTCGAT 
AAGTACTTCA GTGCACCGGG CACCGAGCTT GAGAAATGGA CGCCCACCGA CTGGAAGGAG 
AATCCCAGTT TCCTCGACCT GATCTCCGAC CCAGATCTGA AGCAATGGGG CGTCGAGCTG 
AATAGCATTT GGAAGGACTT GGGACGCAAA ATGAAGGACG AGGTGTCAAA GAATCCCGAA 
TACTACTCAA TCATTCCCGT GCCAAATCCA GTGATCGTGC CCGGCGGTCG TTTCATTGAG 
TTCTACTACT GGGACTCCTA CTGGATCATC CGTGGACTTC TCTACAGCCA GATGTTTGAC 
ACCGCGCGCG GCATGATTGA GAACTTCTTT TCCATTGTCA ATCGGTTCGG TTTTATTCCA 
AACGGCGGTC GAGTCTACTA CCACGGTCGC TCCCAGCCGC CACTTCTAAC CGGTATGGTC 
AAGTCGTACG TGGACTTCAC CAACGATGAC AAGTTCGCCA TCGATGCCCT GGACACGCTG 
GAGCACGAGT TCGAGTTCTT TGTGAACAAC CACAATGTCA CGGTGAAGAA TCACAGCCTG 
TGTGTGTACC GCGATTCGTC GTCCGGACCG CGACCAGAAT CCTACCGAGA GGATGTGGAG 
ACCGGCGAGG AGTTCCCCAC GGATGAGGCC AAGGAACTGC ATTACAGTGA ACTCAAGGCA 
GGCGCCGAAT CGGGCATGGA CTTTAGCTCG CGCTGGTTCA TCTCACCGAC TGGAACCAAT 
GATGGCAACC GGAGCGCTCT GAGCACCACC TCCATTGTGC CCGTCGACCT GAATGCCTAT 
CTCTACTGGA ACGCCAAGTT GATTGCCGAG TTCCATTCCA AAGCGGGCAA CACCAAAAAG 
GTCACCGAAT ACGAGACCAA GGCCGAGAAA CTCCTTCTGG GTATCCAAGA AGTTTTGTGG 
AACGAGGAGG CCGGTGTCTG GTTGGACTAC GATATGATTA ACCAGAAGCC TCGCGATTAC 
TACACGCCCA CCAATCTATC TCCACTGTGG GTGAAGGCCT TCAACATTTC GGAGTCCGAA 
AAGATATCGG CTTCGGTTAT GGCCTACATT GAGAGGAACA AGCTGGACAG CTTCCCTGGC 
GGAGTTCCCA ACACGCTGAG CTACACCGGA GAACAGTGGG ATGCCCCCAA TGTGTGGGCA 
CCGATGCAGT ACATCCTGGT CGAGGGCCTA AACAACCTGA ACACTCCCGA GGCCAAGAAT 
ATGTCACTGA AGTGGGCCAC CAGGTGGGTG AAGACAAACT TTGCGGCGTT TAGCAAGGAC 
AGGCACATGT ACGAGAAGTA CAACGCCGAT GAGTTCGGAG TTGGAGGCGG CGGTGGCGAG 
TACGAGGTAC AGACTGGATT CGGTTGGTCC AACGGTGTGA TCATCGAGTG GCTGAGCAAG 
CACGGGCGCG ACATTTCCAT TGGATCCGGT TGTGGCTGCC TAGCAGGTGA AAAGCGGCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Treh ( NM_166423.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726025.1 Drosophila melanogaster trehalase, transcript variant C (Treh), mRNA.

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CloneID	OFa11401
Clone ID Related Accession (Same CDS sequence)	NM_166423.3 , NM_166424.2
Accession Version	NM_166423.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166423.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAAGC TACCGACCAT CAGCCTGCTC CTCGTGAGCT GGAGTTGCCT GGTGGCCTTG 
TCCCAGGCCA AGACCTACAG CCTGCCCGAT CTCACCACCG ACTATAACAA TGCCATTCCC 
GTCGACGAGG AGGAGGCACA GGATCCGTTC GCCAGCTGTA AAATCTACTG CGAGGGCAAT 
CTGCTGCACA CCATCCAAAC GGCAGTGCCC AAACTATTTG CGGATTCGAA AACGTTTGTG 
GACATGAAGC TGAACAATTC GCCCGACAAG ACCCTCGAGG ACTTTAATGC CATGATGGAG 
GCCAAGAATC AGACGCCAAG CAGTGAGGAT CTCAAGCAGT TTGTCGATAA GTACTTCAGT 
GCACCGGGCA CCGAGCTTGA GAAATGGACG CCCACCGACT GGAAGGAGAA TCCCAGTTTC 
CTCGACCTGA TCTCCGACCC AGATCTGAAG CAATGGGGCG TCGAGCTGAA TAGCATTTGG 
AAGGACTTGG GACGCAAAAT GAAGGACGAG GTGTCAAAGA ATCCCGAATA CTACTCAATC 
ATTCCCGTGC CAAATCCAGT GATCGTGCCC GGCGGTCGTT TCATTGAGTT CTACTACTGG 
GACTCCTACT GGATCATCCG TGGACTTCTC TACAGCCAGA TGTTTGACAC CGCGCGCGGC 
ATGATTGAGA ACTTCTTTTC CATTGTCAAT CGGTTCGGTT TTATTCCAAA CGGCGGTCGA 
GTCTACTACC ACGGTCGCTC CCAGCCGCCA CTTCTAACCG GTATGGTCAA GTCGTACGTG 
GACTTCACCA ACGATGACAA GTTCGCCATC GATGCCCTGG ACACGCTGGA GCACGAGTTC 
GAGTTCTTTG TGAACAACCA CAATGTCACG GTGAAGAATC ACAGCCTGTG TGTGTACCGC 
GATTCGTCGT CCGGACCGCG ACCAGAATCC TACCGAGAGG ATGTGGAGAC CGGCGAGGAG 
TTCCCCACGG ATGAGGCCAA GGAACTGCAT TACAGTGAAC TCAAGGCAGG CGCCGAATCG 
GGCATGGACT TTAGCTCGCG CTGGTTCATC TCACCGACTG GAACCAATGA TGGCAACCGG 
AGCGCTCTGA GCACCACCTC CATTGTGCCC GTCGACCTGA ATGCCTATCT CTACTGGAAC 
GCCAAGTTGA TTGCCGAGTT CCATTCCAAA GCGGGCAACA CCAAAAAGGT CACCGAATAC 
GAGACCAAGG CCGAGAAACT CCTTCTGGGT ATCCAAGAAG TTTTGTGGAA CGAGGAGGCC 
GGTGTCTGGT TGGACTACGA TATGATTAAC CAGAAGCCTC GCGATTACTA CACGCCCACC 
AATCTATCTC CACTGTGGGT GAAGGCCTTC AACATTTCGG AGTCCGAAAA GATATCGGCT 
TCGGTTATGG CCTACATTGA GAGGAACAAG CTGGACAGCT TCCCTGGCGG AGTTCCCAAC 
ACGCTGAGCT ACACCGGAGA ACAGTGGGAT GCCCCCAATG TGTGGGCACC GATGCAGTAC 
ATCCTGGTCG AGGGCCTAAA CAACCTGAAC ACTCCCGAGG CCAAGAATAT GTCACTGAAG 
TGGGCCACCA GGTGGGTGAA GACAAACTTT GCGGCGTTTA GCAAGGACAG GCACATGTAC 
GAGAAGTACA ACGCCGATGA GTTCGGAGTT GGAGGCGGCG GTGGCGAGTA CGAGGTACAG 
ACTGGATTCG GTTGGTCCAA CGGTGTGATC ATCGAGTGGC TGAGCAAGCA CGGGCGCGAC 
ATTTCCATTG GATCCGGTTG TGGCTGCCTA GCAGGTGAAA AGCGGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726025.1
CDS	313..2103
Translation	MFKLPTISLLLVSWSCLVALSQAKTYSLPDLTTDYNNAIPVDEEEAQDPFASCKIYCEGNLLHTIQTAVPKLFADSKTFVDMKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_166423.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant C (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166423.3

ORF Insert Sequence:

ATGTTCAAGC TACCGACCAT CAGCCTGCTC CTCGTGAGCT GGAGTTGCCT GGTGGCCTTG 
TCCCAGGCCA AGACCTACAG CCTGCCCGAT CTCACCACCG ACTATAACAA TGCCATTCCC 
GTCGACGAGG AGGAGGCACA GGATCCGTTC GCCAGCTGTA AAATCTACTG CGAGGGCAAT 
CTGCTGCACA CCATCCAAAC GGCAGTGCCC AAACTATTTG CGGATTCGAA AACGTTTGTG 
GACATGAAGC TGAACAATTC GCCCGACAAG ACCCTCGAGG ACTTTAATGC CATGATGGAG 
GCCAAGAATC AGACGCCAAG CAGTGAGGAT CTCAAGCAGT TTGTCGATAA GTACTTCAGT 
GCACCGGGCA CCGAGCTTGA GAAATGGACG CCCACCGACT GGAAGGAGAA TCCCAGTTTC 
CTCGACCTGA TCTCCGACCC AGATCTGAAG CAATGGGGCG TCGAGCTGAA TAGCATTTGG 
AAGGACTTGG GACGCAAAAT GAAGGACGAG GTGTCAAAGA ATCCCGAATA CTACTCAATC 
ATTCCCGTGC CAAATCCAGT GATCGTGCCC GGCGGTCGTT TCATTGAGTT CTACTACTGG 
GACTCCTACT GGATCATCCG TGGACTTCTC TACAGCCAGA TGTTTGACAC CGCGCGCGGC 
ATGATTGAGA ACTTCTTTTC CATTGTCAAT CGGTTCGGTT TTATTCCAAA CGGCGGTCGA 
GTCTACTACC ACGGTCGCTC CCAGCCGCCA CTTCTAACCG GTATGGTCAA GTCGTACGTG 
GACTTCACCA ACGATGACAA GTTCGCCATC GATGCCCTGG ACACGCTGGA GCACGAGTTC 
GAGTTCTTTG TGAACAACCA CAATGTCACG GTGAAGAATC ACAGCCTGTG TGTGTACCGC 
GATTCGTCGT CCGGACCGCG ACCAGAATCC TACCGAGAGG ATGTGGAGAC CGGCGAGGAG 
TTCCCCACGG ATGAGGCCAA GGAACTGCAT TACAGTGAAC TCAAGGCAGG CGCCGAATCG 
GGCATGGACT TTAGCTCGCG CTGGTTCATC TCACCGACTG GAACCAATGA TGGCAACCGG 
AGCGCTCTGA GCACCACCTC CATTGTGCCC GTCGACCTGA ATGCCTATCT CTACTGGAAC 
GCCAAGTTGA TTGCCGAGTT CCATTCCAAA GCGGGCAACA CCAAAAAGGT CACCGAATAC 
GAGACCAAGG CCGAGAAACT CCTTCTGGGT ATCCAAGAAG TTTTGTGGAA CGAGGAGGCC 
GGTGTCTGGT TGGACTACGA TATGATTAAC CAGAAGCCTC GCGATTACTA CACGCCCACC 
AATCTATCTC CACTGTGGGT GAAGGCCTTC AACATTTCGG AGTCCGAAAA GATATCGGCT 
TCGGTTATGG CCTACATTGA GAGGAACAAG CTGGACAGCT TCCCTGGCGG AGTTCCCAAC 
ACGCTGAGCT ACACCGGAGA ACAGTGGGAT GCCCCCAATG TGTGGGCACC GATGCAGTAC 
ATCCTGGTCG AGGGCCTAAA CAACCTGAAC ACTCCCGAGG CCAAGAATAT GTCACTGAAG 
TGGGCCACCA GGTGGGTGAA GACAAACTTT GCGGCGTTTA GCAAGGACAG GCACATGTAC 
GAGAAGTACA ACGCCGATGA GTTCGGAGTT GGAGGCGGCG GTGGCGAGTA CGAGGTACAG 
ACTGGATTCG GTTGGTCCAA CGGTGTGATC ATCGAGTGGC TGAGCAAGCA CGGGCGCGAC 
ATTTCCATTG GATCCGGTTG TGGCTGCCTA GCAGGTGAAA AGCGGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Treh ( NM_166424.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726026.1 Drosophila melanogaster trehalase, transcript variant F (Treh), mRNA.

Price & Availability↑

CloneID	OFa11401
Clone ID Related Accession (Same CDS sequence)	NM_166423.3 , NM_166424.2
Accession Version	NM_166424.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166424.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAAGC TACCGACCAT CAGCCTGCTC CTCGTGAGCT GGAGTTGCCT GGTGGCCTTG 
TCCCAGGCCA AGACCTACAG CCTGCCCGAT CTCACCACCG ACTATAACAA TGCCATTCCC 
GTCGACGAGG AGGAGGCACA GGATCCGTTC GCCAGCTGTA AAATCTACTG CGAGGGCAAT 
CTGCTGCACA CCATCCAAAC GGCAGTGCCC AAACTATTTG CGGATTCGAA AACGTTTGTG 
GACATGAAGC TGAACAATTC GCCCGACAAG ACCCTCGAGG ACTTTAATGC CATGATGGAG 
GCCAAGAATC AGACGCCAAG CAGTGAGGAT CTCAAGCAGT TTGTCGATAA GTACTTCAGT 
GCACCGGGCA CCGAGCTTGA GAAATGGACG CCCACCGACT GGAAGGAGAA TCCCAGTTTC 
CTCGACCTGA TCTCCGACCC AGATCTGAAG CAATGGGGCG TCGAGCTGAA TAGCATTTGG 
AAGGACTTGG GACGCAAAAT GAAGGACGAG GTGTCAAAGA ATCCCGAATA CTACTCAATC 
ATTCCCGTGC CAAATCCAGT GATCGTGCCC GGCGGTCGTT TCATTGAGTT CTACTACTGG 
GACTCCTACT GGATCATCCG TGGACTTCTC TACAGCCAGA TGTTTGACAC CGCGCGCGGC 
ATGATTGAGA ACTTCTTTTC CATTGTCAAT CGGTTCGGTT TTATTCCAAA CGGCGGTCGA 
GTCTACTACC ACGGTCGCTC CCAGCCGCCA CTTCTAACCG GTATGGTCAA GTCGTACGTG 
GACTTCACCA ACGATGACAA GTTCGCCATC GATGCCCTGG ACACGCTGGA GCACGAGTTC 
GAGTTCTTTG TGAACAACCA CAATGTCACG GTGAAGAATC ACAGCCTGTG TGTGTACCGC 
GATTCGTCGT CCGGACCGCG ACCAGAATCC TACCGAGAGG ATGTGGAGAC CGGCGAGGAG 
TTCCCCACGG ATGAGGCCAA GGAACTGCAT TACAGTGAAC TCAAGGCAGG CGCCGAATCG 
GGCATGGACT TTAGCTCGCG CTGGTTCATC TCACCGACTG GAACCAATGA TGGCAACCGG 
AGCGCTCTGA GCACCACCTC CATTGTGCCC GTCGACCTGA ATGCCTATCT CTACTGGAAC 
GCCAAGTTGA TTGCCGAGTT CCATTCCAAA GCGGGCAACA CCAAAAAGGT CACCGAATAC 
GAGACCAAGG CCGAGAAACT CCTTCTGGGT ATCCAAGAAG TTTTGTGGAA CGAGGAGGCC 
GGTGTCTGGT TGGACTACGA TATGATTAAC CAGAAGCCTC GCGATTACTA CACGCCCACC 
AATCTATCTC CACTGTGGGT GAAGGCCTTC AACATTTCGG AGTCCGAAAA GATATCGGCT 
TCGGTTATGG CCTACATTGA GAGGAACAAG CTGGACAGCT TCCCTGGCGG AGTTCCCAAC 
ACGCTGAGCT ACACCGGAGA ACAGTGGGAT GCCCCCAATG TGTGGGCACC GATGCAGTAC 
ATCCTGGTCG AGGGCCTAAA CAACCTGAAC ACTCCCGAGG CCAAGAATAT GTCACTGAAG 
TGGGCCACCA GGTGGGTGAA GACAAACTTT GCGGCGTTTA GCAAGGACAG GCACATGTAC 
GAGAAGTACA ACGCCGATGA GTTCGGAGTT GGAGGCGGCG GTGGCGAGTA CGAGGTACAG 
ACTGGATTCG GTTGGTCCAA CGGTGTGATC ATCGAGTGGC TGAGCAAGCA CGGGCGCGAC 
ATTTCCATTG GATCCGGTTG TGGCTGCCTA GCAGGTGAAA AGCGGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726026.1
CDS	207..1997
Translation	MFKLPTISLLLVSWSCLVALSQAKTYSLPDLTTDYNNAIPVDEEEAQDPFASCKIYCEGNLLHTIQTAVPKLFADSKTFVDMKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_166424.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant F (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166424.2

ORF Insert Sequence:

ATGTTCAAGC TACCGACCAT CAGCCTGCTC CTCGTGAGCT GGAGTTGCCT GGTGGCCTTG 
TCCCAGGCCA AGACCTACAG CCTGCCCGAT CTCACCACCG ACTATAACAA TGCCATTCCC 
GTCGACGAGG AGGAGGCACA GGATCCGTTC GCCAGCTGTA AAATCTACTG CGAGGGCAAT 
CTGCTGCACA CCATCCAAAC GGCAGTGCCC AAACTATTTG CGGATTCGAA AACGTTTGTG 
GACATGAAGC TGAACAATTC GCCCGACAAG ACCCTCGAGG ACTTTAATGC CATGATGGAG 
GCCAAGAATC AGACGCCAAG CAGTGAGGAT CTCAAGCAGT TTGTCGATAA GTACTTCAGT 
GCACCGGGCA CCGAGCTTGA GAAATGGACG CCCACCGACT GGAAGGAGAA TCCCAGTTTC 
CTCGACCTGA TCTCCGACCC AGATCTGAAG CAATGGGGCG TCGAGCTGAA TAGCATTTGG 
AAGGACTTGG GACGCAAAAT GAAGGACGAG GTGTCAAAGA ATCCCGAATA CTACTCAATC 
ATTCCCGTGC CAAATCCAGT GATCGTGCCC GGCGGTCGTT TCATTGAGTT CTACTACTGG 
GACTCCTACT GGATCATCCG TGGACTTCTC TACAGCCAGA TGTTTGACAC CGCGCGCGGC 
ATGATTGAGA ACTTCTTTTC CATTGTCAAT CGGTTCGGTT TTATTCCAAA CGGCGGTCGA 
GTCTACTACC ACGGTCGCTC CCAGCCGCCA CTTCTAACCG GTATGGTCAA GTCGTACGTG 
GACTTCACCA ACGATGACAA GTTCGCCATC GATGCCCTGG ACACGCTGGA GCACGAGTTC 
GAGTTCTTTG TGAACAACCA CAATGTCACG GTGAAGAATC ACAGCCTGTG TGTGTACCGC 
GATTCGTCGT CCGGACCGCG ACCAGAATCC TACCGAGAGG ATGTGGAGAC CGGCGAGGAG 
TTCCCCACGG ATGAGGCCAA GGAACTGCAT TACAGTGAAC TCAAGGCAGG CGCCGAATCG 
GGCATGGACT TTAGCTCGCG CTGGTTCATC TCACCGACTG GAACCAATGA TGGCAACCGG 
AGCGCTCTGA GCACCACCTC CATTGTGCCC GTCGACCTGA ATGCCTATCT CTACTGGAAC 
GCCAAGTTGA TTGCCGAGTT CCATTCCAAA GCGGGCAACA CCAAAAAGGT CACCGAATAC 
GAGACCAAGG CCGAGAAACT CCTTCTGGGT ATCCAAGAAG TTTTGTGGAA CGAGGAGGCC 
GGTGTCTGGT TGGACTACGA TATGATTAAC CAGAAGCCTC GCGATTACTA CACGCCCACC 
AATCTATCTC CACTGTGGGT GAAGGCCTTC AACATTTCGG AGTCCGAAAA GATATCGGCT 
TCGGTTATGG CCTACATTGA GAGGAACAAG CTGGACAGCT TCCCTGGCGG AGTTCCCAAC 
ACGCTGAGCT ACACCGGAGA ACAGTGGGAT GCCCCCAATG TGTGGGCACC GATGCAGTAC 
ATCCTGGTCG AGGGCCTAAA CAACCTGAAC ACTCCCGAGG CCAAGAATAT GTCACTGAAG 
TGGGCCACCA GGTGGGTGAA GACAAACTTT GCGGCGTTTA GCAAGGACAG GCACATGTAC 
GAGAAGTACA ACGCCGATGA GTTCGGAGTT GGAGGCGGCG GTGGCGAGTA CGAGGTACAG 
ACTGGATTCG GTTGGTCCAA CGGTGTGATC ATCGAGTGGC TGAGCAAGCA CGGGCGCGAC 
ATTTCCATTG GATCCGGTTG TGGCTGCCTA GCAGGTGAAA AGCGGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Treh ( NM_166425.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726027.1 Drosophila melanogaster trehalase, transcript variant B (Treh), mRNA.

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CloneID	OFa11402
Clone ID Related Accession (Same CDS sequence)	NM_166425.2
Accession Version	NM_166425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	trehalase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_166425.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGA ACAATTCGCC CGACAAGACC CTCGAGGACT TTAATGCCAT GATGGAGGCC 
AAGAATCAGA CGCCAAGCAG TGAGGATCTC AAGCAGTTTG TCGATAAGTA CTTCAGTGCA 
CCGGGCACCG AGCTTGAGAA ATGGACGCCC ACCGACTGGA AGGAGAATCC CAGTTTCCTC 
GACCTGATCT CCGACCCAGA TCTGAAGCAA TGGGGCGTCG AGCTGAATAG CATTTGGAAG 
GACTTGGGAC GCAAAATGAA GGACGAGGTG TCAAAGAATC CCGAATACTA CTCAATCATT 
CCCGTGCCAA ATCCAGTGAT CGTGCCCGGC GGTCGTTTCA TTGAGTTCTA CTACTGGGAC 
TCCTACTGGA TCATCCGTGG ACTTCTCTAC AGCCAGATGT TTGACACCGC GCGCGGCATG 
ATTGAGAACT TCTTTTCCAT TGTCAATCGG TTCGGTTTTA TTCCAAACGG CGGTCGAGTC 
TACTACCACG GTCGCTCCCA GCCGCCACTT CTAACCGGTA TGGTCAAGTC GTACGTGGAC 
TTCACCAACG ATGACAAGTT CGCCATCGAT GCCCTGGACA CGCTGGAGCA CGAGTTCGAG 
TTCTTTGTGA ACAACCACAA TGTCACGGTG AAGAATCACA GCCTGTGTGT GTACCGCGAT 
TCGTCGTCCG GACCGCGACC AGAATCCTAC CGAGAGGATG TGGAGACCGG CGAGGAGTTC 
CCCACGGATG AGGCCAAGGA ACTGCATTAC AGTGAACTCA AGGCAGGCGC CGAATCGGGC 
ATGGACTTTA GCTCGCGCTG GTTCATCTCA CCGACTGGAA CCAATGATGG CAACCGGAGC 
GCTCTGAGCA CCACCTCCAT TGTGCCCGTC GACCTGAATG CCTATCTCTA CTGGAACGCC 
AAGTTGATTG CCGAGTTCCA TTCCAAAGCG GGCAACACCA AAAAGGTCAC CGAATACGAG 
ACCAAGGCCG AGAAACTCCT TCTGGGTATC CAAGAAGTTT TGTGGAACGA GGAGGCCGGT 
GTCTGGTTGG ACTACGATAT GATTAACCAG AAGCCTCGCG ATTACTACAC GCCCACCAAT 
CTATCTCCAC TGTGGGTGAA GGCCTTCAAC ATTTCGGAGT CCGAAAAGAT ATCGGCTTCG 
GTTATGGCCT ACATTGAGAG GAACAAGCTG GACAGCTTCC CTGGCGGAGT TCCCAACACG 
CTGAGCTACA CCGGAGAACA GTGGGATGCC CCCAATGTGT GGGCACCGAT GCAGTACATC 
CTGGTCGAGG GCCTAAACAA CCTGAACACT CCCGAGGCCA AGAATATGTC ACTGAAGTGG 
GCCACCAGGT GGGTGAAGAC AAACTTTGCG GCGTTTAGCA AGGACAGGCA CATGTACGAG 
AAGTACAACG CCGATGAGTT CGGAGTTGGA GGCGGCGGTG GCGAGTACGA GGTACAGACT 
GGATTCGGTT GGTCCAACGG TGTGATCATC GAGTGGCTGA GCAAGCACGG GCGCGACATT 
TCCATTGGAT CCGGTTGTGG CTGCCTAGCA GGTGAAAAGC GGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726027.1
CDS	290..1837
Translation	MKLNNSPDKTLEDFNAMMEAKNQTPSSEDLKQFVDKYFSAPGTELEKWTPTDWKENPSFLDLISDPDLKQWGVELNSIWKDLGRKMKDEVSKNPEYYSIIPVPNPVIVPGGRFIEFYYWDSYWIIRGLLYSQMFDTARGMIENFFSIVNRFGFIPNGGRVYYHGRSQPPLLTGMVKSYVDFTNDDKFAIDALDTLEHEFEFFVNNHNVTVKNHSLCVYRDSSSGPRPESYREDVETGEEFPTDEAKELHYSELKAGAESGMDFSSRWFISPTGTNDGNRSALSTTSIVPVDLNAYLYWNAKLIAEFHSKAGNTKKVTEYETKAEKLLLGIQEVLWNEEAGVWLDYDMINQKPRDYYTPTNLSPLWVKAFNISESEKISASVMAYIERNKLDSFPGGVPNTLSYTGEQWDAPNVWAPMQYILVEGLNNLNTPEAKNMSLKWATRWVKTNFAAFSKDRHMYEKYNADEFGVGGGGGEYEVQTGFGWSNGVIIEWLSKHGRDISIGSGCGCLAGEKRQ

Target ORF information:

RefSeq Version	NM_166425.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster trehalase, transcript variant B (Treh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166425.2

ORF Insert Sequence:

ATGAAGCTGA ACAATTCGCC CGACAAGACC CTCGAGGACT TTAATGCCAT GATGGAGGCC 
AAGAATCAGA CGCCAAGCAG TGAGGATCTC AAGCAGTTTG TCGATAAGTA CTTCAGTGCA 
CCGGGCACCG AGCTTGAGAA ATGGACGCCC ACCGACTGGA AGGAGAATCC CAGTTTCCTC 
GACCTGATCT CCGACCCAGA TCTGAAGCAA TGGGGCGTCG AGCTGAATAG CATTTGGAAG 
GACTTGGGAC GCAAAATGAA GGACGAGGTG TCAAAGAATC CCGAATACTA CTCAATCATT 
CCCGTGCCAA ATCCAGTGAT CGTGCCCGGC GGTCGTTTCA TTGAGTTCTA CTACTGGGAC 
TCCTACTGGA TCATCCGTGG ACTTCTCTAC AGCCAGATGT TTGACACCGC GCGCGGCATG 
ATTGAGAACT TCTTTTCCAT TGTCAATCGG TTCGGTTTTA TTCCAAACGG CGGTCGAGTC 
TACTACCACG GTCGCTCCCA GCCGCCACTT CTAACCGGTA TGGTCAAGTC GTACGTGGAC 
TTCACCAACG ATGACAAGTT CGCCATCGAT GCCCTGGACA CGCTGGAGCA CGAGTTCGAG 
TTCTTTGTGA ACAACCACAA TGTCACGGTG AAGAATCACA GCCTGTGTGT GTACCGCGAT 
TCGTCGTCCG GACCGCGACC AGAATCCTAC CGAGAGGATG TGGAGACCGG CGAGGAGTTC 
CCCACGGATG AGGCCAAGGA ACTGCATTAC AGTGAACTCA AGGCAGGCGC CGAATCGGGC 
ATGGACTTTA GCTCGCGCTG GTTCATCTCA CCGACTGGAA CCAATGATGG CAACCGGAGC 
GCTCTGAGCA CCACCTCCAT TGTGCCCGTC GACCTGAATG CCTATCTCTA CTGGAACGCC 
AAGTTGATTG CCGAGTTCCA TTCCAAAGCG GGCAACACCA AAAAGGTCAC CGAATACGAG 
ACCAAGGCCG AGAAACTCCT TCTGGGTATC CAAGAAGTTT TGTGGAACGA GGAGGCCGGT 
GTCTGGTTGG ACTACGATAT GATTAACCAG AAGCCTCGCG ATTACTACAC GCCCACCAAT 
CTATCTCCAC TGTGGGTGAA GGCCTTCAAC ATTTCGGAGT CCGAAAAGAT ATCGGCTTCG 
GTTATGGCCT ACATTGAGAG GAACAAGCTG GACAGCTTCC CTGGCGGAGT TCCCAACACG 
CTGAGCTACA CCGGAGAACA GTGGGATGCC CCCAATGTGT GGGCACCGAT GCAGTACATC 
CTGGTCGAGG GCCTAAACAA CCTGAACACT CCCGAGGCCA AGAATATGTC ACTGAAGTGG 
GCCACCAGGT GGGTGAAGAC AAACTTTGCG GCGTTTAGCA AGGACAGGCA CATGTACGAG 
AAGTACAACG CCGATGAGTT CGGAGTTGGA GGCGGCGGTG GCGAGTACGA GGTACAGACT 
GGATTCGGTT GGTCCAACGG TGTGATCATC GAGTGGCTGA GCAAGCACGG GCGCGACATT 
TCCATTGGAT CCGGTTGTGG CTGCCTAGCA GGTGAAAAGC GGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Treh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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