lin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	lin
Entrez Gene ID	45325
Full Name	lines
Synonyms	CG11770,Dmel\CG11770,Lin
General protein information	Preferred Names lines Names CG11770 gene product from transcript CG11770-RA CG11770-PA lin-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44F6-44F7\|2-59 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_080076.3	NP_524815.1	lines

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	lin	NP_524815.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010757	XP_554294.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lines acts antagonistically to bowl in hub specification, establishing bowl as a positively acting factor in the development of the testis niche.
Title: lines and bowl affect the specification of cyst stem cells and niche cells in the Drosophila testis.

Here we show that Lines accumulates in nuclei in the presumptive tarsus and the inter-joints of proximal leg segments and governs the formation of these structures by destabilizing the nuclear protein Bowl
Title: Essential roles for lines in mediating leg and antennal proximodistal patterning and generating a stable Notch signaling interface at segment borders.

by preventing Bowl accumulation in the wing, primordial Lines permits the correct balance of nuclear co-repressors that control the activity of the Wingless, Notch and Hedgehog pathways
Title: Lines is required for normal operation of Wingless, Hedgehog and Notch pathways during wing development.

Reciprocal roles are reported for bowl and lin in specifying the peripodial epithelum and the disc proper of the wing.
Title: Reciprocal roles for bowl and lines in specifying the peripodial epithelium and the disc proper of the Drosophila wing primordium.

Drm and Lines regulate Bowl protein accumulation and consequent patterning in the epithelia of the foregut, hindgut, and imaginal discs.
Title: The Drumstick/Lines/Bowl regulatory pathway links antagonistic Hedgehog and Wingless signaling inputs to epidermal cell differentiation.

The following lin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11394	NM_080076.3 Latest version!	Drosophila melanogaster lines (lin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lin ( NM_080076.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524815.1 Drosophila melanogaster lines (lin), mRNA.

Price & Availability↑

CloneID	OFa11394
Clone ID Related Accession (Same CDS sequence)	NM_080076.3
Accession Version	NM_080076.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lines
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_080076.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACACCT CATCCGCAGC AGGATCTGGA GCCGGAACGG GCGATGTGTG CCCATGCTCC 
ACGGTAGCAA CCTCCACGAC CTCCGCATCA AACGAACAGC CGCAGACCAA GCGCCAGAAG 
ATCGAGCAGC AAATCAAGGT GGGCTCCAAG GCGCTGCCAC CCCAGCCGCC CCATCCGCCG 
GATATACTGG ACCTGGACGC CAACAGCAAC AGCCACCTGT GCTCCTCGCT CTCCTCGTCC 
TCTTCGCACT CGCTGTCAAC GCCAAGCACC GCCAACAACT CCCCCACCAC CACGCCCTGT 
AGTTCCCGGG CGGCGTCGTA CGCCCAGCTG CTGCACAACA TCTTGCCACA AAACGGGGAC 
ACAGACCTGC ACAGCCAGCC TGAATCCGCC CATGACGAAG TGGACAGGGC GGCCAAATTG 
CCCAACCTGC TAACCCTCGA CACGACCAAC GGCAATGGCA GTCATGCTAG CAGCAGGAGC 
AATTGCTTGC AAGCAACAAC AGCCACGTCC ATCGACGATA TGCTGGAGTT CGAGCAGTCC 
CTGACCCGCC AGTGCCTGTG CGGCGTCTCG GAGCGAACGC TGCGCAAGCC CTTCCAGTCG 
CATTACTCAC AGGACACCAA CGGCCAGAAA CGGATAGCCT ACCTGCGGGA GTGGCCCACC 
AACAAGCTGC TGCAGTTTCT GTCGAATCTG CAGCTACTGT TCGATATCTA CCTGAAGCAG 
AACGCAAAGG GCTTCATCTG CACGCGGATA ATGGACGTTT GCGACGCACT CATTCGCAAT 
GACCACAAGC TTATCGACGA GATTATCGTG CTGGCCGGCT ACGAGAACTC TTACGTGCAA 
TTTCTGGCGG GACGTGTGCT AGCCGCATTC CTGGTGATTG CCAAGCAAGA GCTGAATGAC 
GAGTGGCTGC AGAAGATCGT CGACCAGCTA TTTAACTTCG AGCAGCTTGA CCAGGCGGCC 
GTGCAGAAGA TCCACTTCAG CCTGGACATC ATCAAGCGCA TAGTGGAGTG GAAGGATATG 
GAGATCCATC CGCTGGACGA CGACTGGATG GCGTCGGCGA ATAGTACAAG TGCAGCCTCG 
TCAGTAGTGC CCCTTTCGAC AGAGGCGTCC GTGAGCTACA TGCATTTTCC CGTGCAGGAG 
CAGCCGCTAG CCACCAATTA TTTTGCACTG CAATTTCGCG AGGATACGGA AGGTGAACGA 
GAGACGGGGC AGGAAGCACC TGACAACCGC GACCGACATC GGCGGCACTT TGGCGAGGAT 
ATGAACGTCG CCTACGAGCC GCACCCCGCT CCCCAGTCCA CGACCATGCC CAGCGGTTGC 
CATGTGGTCA CACTAACCGA CTCGGAAAGC TTCGATACGA CGCACCTGAA GTGCATCACC 
ATTCAAAAGC TGGAGCACAA GTGGCCCACG CTGGTGAAGA ACATGTCGGA GTTGATGGCG 
CCGACGCATC AGGATGCGGC CGAGCACTGT GTGCTCAACT TCTTGCAGCT ATGGGAAAAC 
ATCATCTCGG TGAAGGCCAA TCTGTCCATC GACGAGACGC GACCCTACTA CGCCCAGCTG 
GACAAGTTTG AGCTGCTGCT CAGCCACAGC CTCTCCTGCA CCGTGTACAA GCAGATGCTG 
TGCCTCTTCA ACGAGGCATT GTGCTATGGT TCCACGCTGG CGCTGCAGGA CATGCTGCCC 
GAGGAGACGT GCAAGCTGGC TCACCAGATA GTCTGCCATG TGCGCGGCTT CCGCATACTG 
GAGTCGTTGC CGCGGCGACA GCCGGACAAC ATGGTCAGTC TGATCGGCTA CAATGGCAAG 
CCGATGGTGT ACGCCAATGG AACAATTACC CTGGCACATG CCGCGCAATC AGGGGACAGC 
GAAGAGGACG GTGCGCCACT GGACCTTATC GAAATGGATA AGACACTGTT GCAGAAGATG 
GTGCTGTTGG TGCTGAAGTC GATAGCGGTG ACGGTGAAGG AGATACGCAG CGATTCCTCC 
GACTCATCCA TCGATTCCAC CGACTACGAT GCCTTCCAGG ACATGATGCT CATTGAGCGC 
TCGATCCGCG ATGTACTCAG CAAGCTGGAG ACGTTCATAA AGCAGACGCT GGAATTCCAT 
CCGGAGTGCC ACTTCAGCAA GATCCTGATC CACCTGTTCG ACGACCAGGA CGACCACTTG 
ATCGAGGCCA TGGTCTGCAC GCTGGACGTC ACGTCTGGGA TATCGTTCCG CAACAACGCC 
TTCCCCGAGC TGGTGGCCAT GCTGAATCCG GTGTACACAT TCCTGGAGTT CCTCAAGATG 
ACATCGAACA GCTCGGACCT GCTGCTGGAC CTGCTGGTGA GCAACGAGAC CTGCTTCCTG 
CTCTACCTGC TGCGCCTGCT CAAGTACATC CGCATGAACT GGACGATGTT CGTGCACAGC 
TGTCACACCT TCGGCATGGG CAGCGCCATG CTAGACGAGG CGATGGGCGT TCTTATCCGG 
CTGCGCCTGC AGATATCCCG CCTCGTGTCG CGCCAGCTCT ACCCCTACGA CATTTCCCCC 
GTGCTGCGCC TGCTCGAGAG CTGCGAGAGC CTGTACGAGG GCAACGAGCT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524815.1
CDS	521..3097
Translation	MDTSSAAGSGAGTGDVCPCSTVATSTTSASNEQPQTKRQKIEQQIKVGSKALPPQPPHPPDILDLDANSNSHLCSSLSSSSSHSLSTPSTANNSPTTTPCSSRAASYAQLLHNILPQNGDTDLHSQPESAHDEVDRAAKLPNLLTLDTTNGNGSHASSRSNCLQATTATSIDDMLEFEQSLTRQCLCGVSERTLRKPFQSHYSQDTNGQKRIAYLREWPTNKLLQFLSNLQLLFDIYLKQNAKGFICTRIMDVCDALIRNDHKLIDEIIVLAGYENSYVQFLAGRVLAAFLVIAKQELNDEWLQKIVDQLFNFEQLDQAAVQKIHFSLDIIKRIVEWKDMEIHPLDDDWMASANSTSAASSVVPLSTEASVSYMHFPVQEQPLATNYFALQFREDTEGERETGQEAPDNRDRHRRHFGEDMNVAYEPHPAPQSTTMPSGCHVVTLTDSESFDTTHLKCITIQKLEHKWPTLVKNMSELMAPTHQDAAEHCVLNFLQLWENIISVKANLSIDETRPYYAQLDKFELLLSHSLSCTVYKQMLCLFNEALCYGSTLALQDMLPEETCKLAHQIVCHVRGFRILESLPRRQPDNMVSLIGYNGKPMVYANGTITLAHAAQSGDSEEDGAPLDLIEMDKTLLQKMVLLVLKSIAVTVKEIRSDSSDSSIDSTDYDAFQDMMLIERSIRDVLSKLETFIKQTLEFHPECHFSKILIHLFDDQDDHLIEAMVCTLDVTSGISFRNNAFPELVAMLNPVYTFLEFLKMTSNSSDLLLDLLVSNETCFLLYLLRLLKYIRMNWTMFVHSCHTFGMGSAMLDEAMGVLIRLRLQISRLVSRQLYPYDISPVLRLLESCESLYEGNELS

Target ORF information:

RefSeq Version	NM_080076.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lines (lin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080076.3

ORF Insert Sequence:

ATGGACACCT CATCCGCAGC AGGATCTGGA GCCGGAACGG GCGATGTGTG CCCATGCTCC 
ACGGTAGCAA CCTCCACGAC CTCCGCATCA AACGAACAGC CGCAGACCAA GCGCCAGAAG 
ATCGAGCAGC AAATCAAGGT GGGCTCCAAG GCGCTGCCAC CCCAGCCGCC CCATCCGCCG 
GATATACTGG ACCTGGACGC CAACAGCAAC AGCCACCTGT GCTCCTCGCT CTCCTCGTCC 
TCTTCGCACT CGCTGTCAAC GCCAAGCACC GCCAACAACT CCCCCACCAC CACGCCCTGT 
AGTTCCCGGG CGGCGTCGTA CGCCCAGCTG CTGCACAACA TCTTGCCACA AAACGGGGAC 
ACAGACCTGC ACAGCCAGCC TGAATCCGCC CATGACGAAG TGGACAGGGC GGCCAAATTG 
CCCAACCTGC TAACCCTCGA CACGACCAAC GGCAATGGCA GTCATGCTAG CAGCAGGAGC 
AATTGCTTGC AAGCAACAAC AGCCACGTCC ATCGACGATA TGCTGGAGTT CGAGCAGTCC 
CTGACCCGCC AGTGCCTGTG CGGCGTCTCG GAGCGAACGC TGCGCAAGCC CTTCCAGTCG 
CATTACTCAC AGGACACCAA CGGCCAGAAA CGGATAGCCT ACCTGCGGGA GTGGCCCACC 
AACAAGCTGC TGCAGTTTCT GTCGAATCTG CAGCTACTGT TCGATATCTA CCTGAAGCAG 
AACGCAAAGG GCTTCATCTG CACGCGGATA ATGGACGTTT GCGACGCACT CATTCGCAAT 
GACCACAAGC TTATCGACGA GATTATCGTG CTGGCCGGCT ACGAGAACTC TTACGTGCAA 
TTTCTGGCGG GACGTGTGCT AGCCGCATTC CTGGTGATTG CCAAGCAAGA GCTGAATGAC 
GAGTGGCTGC AGAAGATCGT CGACCAGCTA TTTAACTTCG AGCAGCTTGA CCAGGCGGCC 
GTGCAGAAGA TCCACTTCAG CCTGGACATC ATCAAGCGCA TAGTGGAGTG GAAGGATATG 
GAGATCCATC CGCTGGACGA CGACTGGATG GCGTCGGCGA ATAGTACAAG TGCAGCCTCG 
TCAGTAGTGC CCCTTTCGAC AGAGGCGTCC GTGAGCTACA TGCATTTTCC CGTGCAGGAG 
CAGCCGCTAG CCACCAATTA TTTTGCACTG CAATTTCGCG AGGATACGGA AGGTGAACGA 
GAGACGGGGC AGGAAGCACC TGACAACCGC GACCGACATC GGCGGCACTT TGGCGAGGAT 
ATGAACGTCG CCTACGAGCC GCACCCCGCT CCCCAGTCCA CGACCATGCC CAGCGGTTGC 
CATGTGGTCA CACTAACCGA CTCGGAAAGC TTCGATACGA CGCACCTGAA GTGCATCACC 
ATTCAAAAGC TGGAGCACAA GTGGCCCACG CTGGTGAAGA ACATGTCGGA GTTGATGGCG 
CCGACGCATC AGGATGCGGC CGAGCACTGT GTGCTCAACT TCTTGCAGCT ATGGGAAAAC 
ATCATCTCGG TGAAGGCCAA TCTGTCCATC GACGAGACGC GACCCTACTA CGCCCAGCTG 
GACAAGTTTG AGCTGCTGCT CAGCCACAGC CTCTCCTGCA CCGTGTACAA GCAGATGCTG 
TGCCTCTTCA ACGAGGCATT GTGCTATGGT TCCACGCTGG CGCTGCAGGA CATGCTGCCC 
GAGGAGACGT GCAAGCTGGC TCACCAGATA GTCTGCCATG TGCGCGGCTT CCGCATACTG 
GAGTCGTTGC CGCGGCGACA GCCGGACAAC ATGGTCAGTC TGATCGGCTA CAATGGCAAG 
CCGATGGTGT ACGCCAATGG AACAATTACC CTGGCACATG CCGCGCAATC AGGGGACAGC 
GAAGAGGACG GTGCGCCACT GGACCTTATC GAAATGGATA AGACACTGTT GCAGAAGATG 
GTGCTGTTGG TGCTGAAGTC GATAGCGGTG ACGGTGAAGG AGATACGCAG CGATTCCTCC 
GACTCATCCA TCGATTCCAC CGACTACGAT GCCTTCCAGG ACATGATGCT CATTGAGCGC 
TCGATCCGCG ATGTACTCAG CAAGCTGGAG ACGTTCATAA AGCAGACGCT GGAATTCCAT 
CCGGAGTGCC ACTTCAGCAA GATCCTGATC CACCTGTTCG ACGACCAGGA CGACCACTTG 
ATCGAGGCCA TGGTCTGCAC GCTGGACGTC ACGTCTGGGA TATCGTTCCG CAACAACGCC 
TTCCCCGAGC TGGTGGCCAT GCTGAATCCG GTGTACACAT TCCTGGAGTT CCTCAAGATG 
ACATCGAACA GCTCGGACCT GCTGCTGGAC CTGCTGGTGA GCAACGAGAC CTGCTTCCTG 
CTCTACCTGC TGCGCCTGCT CAAGTACATC CGCATGAACT GGACGATGTT CGTGCACAGC 
TGTCACACCT TCGGCATGGG CAGCGCCATG CTAGACGAGG CGATGGGCGT TCTTATCCGG 
CTGCGCCTGC AGATATCCCG CCTCGTGTCG CGCCAGCTCT ACCCCTACGA CATTTCCCCC 
GTGCTGCGCC TGCTCGAGAG CTGCGAGAGC CTGTACGAGG GCAACGAGCT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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lin cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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