csw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	csw
Entrez Gene ID	45278
Full Name	corkscrew
Synonyms	19-106,CG3954,CSW,Csw,Csw/Shp2,Dmel\CG3954,E(sev)1A,EG:BACN25G24.2,SHP-2,Shp-2,Shp2/csw,anon-WO03040301.207,anon-WO03040301.209,anon-WO03040301.219,csw/SHP-2,l(1)2Db,l(1)2Dd,l(1)G0170,l(1)GA114,l(1)csw
General protein information	Preferred Names corkscrew Names CG3954 gene product from transcript CG3954-RD CG3954-PA CG3954-PB CG3954-PC CG3954-PD csw-PA csw-PB csw-PC csw-PD enhancer of sevenless 1A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 2D1-2D2\|1-1 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272245.1	NP_001259174.1	corkscrew, isoform D
NM_057782.5	NP_477130.1	corkscrew, isoform A
NM_166928.2	NP_726793.1	corkscrew, isoform C
NM_057783.3	NP_477131.1	corkscrew, isoform B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PTPN11	NP_990299.1
Danio rerio (zebrafish)	ptpn11a	NP_956140.1
	PTPN11	XP_001110117.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002438	XP_563746.4
Bos taurus (cattle)	PTPN11	XP_002694636.2
Rattus norvegicus (Norway rat)	Ptpn11	NP_001171064.1
Drosophila melanogaster (fruit fly)	csw	NP_001259174.1
Pan troglodytes (chimpanzee)	PTPN11	XP_522535.3
Canis lupus familiaris (dog)	PTPN11	XP_005636307.1
Homo sapiens (human)	PTPN11	NP_002825.3
Mus musculus (house mouse)	Ptpn11	NP_035332.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that in Drosophila, the duration of resting intervals required for inducing long-term memory is regulated by activity levels of the protein tyrosine phosphatase corkscrew (CSW).
Title: The phosphatase SHP2 regulates the spacing effect for long-term memory induction.

LEOPARD syndrome mutations in csw gene have gain-of-function effects during Drosophila development.
Title: Phosphatase-defective LEOPARD syndrome mutations in PTPN11 gene have gain-of-function effects during Drosophila development.

Corkscrew/SHP-2 proteins control Sprouty phosphorylation and function
Title: Sprouty proteins are in vivo targets of Corkscrew/SHP-2 tyrosine phosphatases.

Data show that Downstream-of-FGFR is a substrate for fibroblast growth factor receptors (FGFRs) and recruits Corkscrew, leading to FGF-induced cell migration and activation of the Ras/MAPK pathway.
Title: Downstream-of-FGFR is a fibroblast growth factor-specific scaffolding protein and recruits Corkscrew upon receptor activation.

Signalling from the pTyr (YB) Neu (ErbB-2) autophosphorylation site was affected by a haplosufficiency in drk (Grb-2), and in genes thought to function downstream of Grb-2: dab, sos, csw (Shp-2), and dos (Gab-1).
Title: Genetic identification of effectors downstream of Neu (ErbB-2) autophosphorylation sites in a Drosophila model.

The following csw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the csw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02376	NM_057782.5 Latest version!	Drosophila melanogaster corkscrew (csw), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa02377	NM_057783.3 Latest version!	Drosophila melanogaster corkscrew (csw), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa02378	NM_166928.2 Latest version!	Drosophila melanogaster corkscrew (csw), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa03748	NM_001272245.1 Latest version!	Drosophila melanogaster corkscrew (csw), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

csw ( NM_057782.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477130.1 Drosophila melanogaster corkscrew (csw), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa02376
Clone ID Related Accession (Same CDS sequence)	NM_057782.5
Accession Version	NM_057782.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	corkscrew, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057782.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCATCGC GAAGATGGTT CCACCCAACG ATATCTGGCA TCGAAGCTGA GAAACTGCTG 
CAGGAGCAGG GATTCGACGG CTCCTTCCTC GCCCGCCTCT CCTCCTCGAA TCCGGGCGCC 
TTCACGCTCT CCGTGCGCCG CGGCAACGAG GTGACCCACA TCAAAATCCA AAACAATGGC 
GACTTCTTTG ATCTCTACGG TGGTGAAAAG TTCGCCACAC TGCCGGAACT GGTACAATAC 
TACATGGAGA ATGGCGAGCT AAAGGAGAAG AACGGCCAGG CCATCGAACT CAAGCAGCCG 
CTGATCTGCG CCGAGCCCAC CACGGAAAGA TGGTTTCATG GCAATCTTTC CGGAAAGGAA 
GCGGAAAAAT TGATCCTGGA GCGGGGCAAG AATGGTTCGT TTCTCGTCCG TGAATCTCAG 
AGCAAGCCTG GCGACTTCGT CCTTTCCGTG CGCACGGACG ACAAAGTAAC GCATGTCATG 
ATTCGATGGC AGGACAAGAA GTACGACGTC GGCGGCGGGG AATCCTTTGG CACCTTGTCG 
GAACTGATCG ATCACTACAA GCGTAATCCC ATGGTGGAGA CGTGCGGAAC CGTGGTGCAT 
CTGCGACAGC CATTCAACGC CACACGAATC ACGGCGGCCG GCATCAATGC CCGGGTGGAA 
CAGCTGGTCA AGGGAGGTTT CTGGGAGGAA TTCGAATCGC TGCAACAGGA CAGTCGGGAC 
ACATTCTCGC GCAACGAGGG CTACAAACAG GAGAACCGCC TCAAGAATCG CTACCGCAAC 
ATATTGCCAT ACGACCACAC GCGCGTCAAG CTGCTGGACG TGGAGCATAG CGTGGCCGGA 
GCCGAGTACA TCAATGCCAA CTACATACGG CTGCCCACCG ACGGCGACCT GTACAACATG 
AGCAGCTCGT CGGAGAGCCT GAACAGCTCG GTGCCCTCGT GCCCCGCCTG CACGGCTGCC 
CAGACACAGC GGAACTGCTC CAACTGCCAG CTGCAAAACA AGACGTGCGT GCAGTGCGCC 
GTGAAGAGCG CCATTCTGCC GTATAGCAAC TGTGCCACCT GCAGCCGCAA GTCAGACTCC 
CTGAGCAAGC ACAAGCGGAG CGAATCCTCG GCCTCTTCAT CGCCCTCCTC CGGCTCTGGG 
TCCGGACCAG GATCGTCGGG CACCAGCGGA GTGAGCAGCG TCAATGGACC CGGCACACCC 
ACCAATCTCA CGAGCGGCAC AGCCGGATGT CTGGTCGGCC TGCTGAAGAG ACACTCGAAC 
GACTCGTCCG GAGCTGTTTC TATATCGATG GCCGAACGGG AACGCGAGAG GGAGCGCGAG 
ATGTTTAAGA CCTACATCGC CACCCAGGGC TGTCTGCTCA CCCAGCAAGT GAACACGGTG 
ACGGACTTCT GGAACATGGT CTGGCAGGAG AACACGCGGG TGATCGTCAT GACCACCAAG 
GAGTACGAGC GCGGCAAAGA AAAGTGCGCC CGCTACTGGC CGGACGAGGG TAGATCGGAG 
CAGTTCGGCC ACGCGCGGAT ACAGTGCGTC TCGGAGAACT CGACCAGTGA CTATACGCTG 
CGCGAGTTCC TCGTCTCGTG GCGGGATCAG CCGGCGCGCC GGATCTTTCA CTACCATTTC 
CAGGTGTGGC CGGATCACGG AGTGCCCGCC GATCCGGGCT GTGTGCTCAA CTTCCTGCAA 
GATGTCAACA CGCGTCAGAG TCACCTGGCT CAAGCGGGCG AGAAGCCGGG TCCGATCTGC 
GTGCACTGCT CTGCGGGCAT CGGTCGCACT GGCACCTTTA TTGTGATCGA TATGATTCTC 
GATCAGATTG TGCGCAATGG ATTGGATACT GAAATCGACA TCCAGCGCAC CATTCAGATG 
GTCCGATCGC AGCGTTCCGG TCTTGTGCAA ACCGAGGCGC AATACAAGTT CGTCTACTAT 
GCGGTGCAGC ACTATATACA GACCCTGATC GCCCGGAAAC GAGCTGAGGA GCAGAGCCTG 
CAGGTTGGCC GCGAGTACAC CAATATAAAG TACACGGGCG AAATTGGAAA CGATTCACAA 
AGATCTCCAT TACCACCAGC AATTTCTAGC ATAAGTTTAG TTCCGAGTAA GACGCCACTG 
ACGCCGACAT CGGCGGATTT GGGCACTGGG ATGGGCCTAA GCATGGGCGT GGGCATGGGC 
GTCGGCAACA AGCACGCATC GAAGCAGCAG CCGCCGTTGC CGGTGGTCAA CTGCAACAAT 
AATAACAACG GCATTGGCAA TAGCGGCTGC AGCAACGGCG GCGGGAGCAG CACCACCAGC 
AGCAGCAACG GCAGCAGCAA CGGTAACATC AACGCCCTAC TGGGCGGCAT CGGCTTGGGG 
CTGGGCGGCA ATATGCGCAA GTCGAACTTT TACAGCGACT CGCTGAAGCA GCAACAGCAG 
CGCGAGGAGC AGGCTCCGGC GGGAGCAGCA AAATTCAAAA ACATTCCCAA AGACATGATC 
GGCTTGCGAC CGCCAAGCCA TGCGCCTGCG TTGCCGCCGC CACCGACACC GCCGCGCAAA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477130.1
CDS	172..2697
Translation	MSSRRWFHPTISGIEAEKLLQEQGFDGSFLARLSSSNPGAFTLSVRRGNEVTHIKIQNNGDFFDLYGGEKFATLPELVQYYMENGELKEKNGQAIELKQPLICAEPTTERWFHGNLSGKEAEKLILERGKNGSFLVRESQSKPGDFVLSVRTDDKVTHVMIRWQDKKYDVGGGESFGTLSELIDHYKRNPMVETCGTVVHLRQPFNATRITAAGINARVEQLVKGGFWEEFESLQQDSRDTFSRNEGYKQENRLKNRYRNILPYDHTRVKLLDVEHSVAGAEYINANYIRLPTDGDLYNMSSSSESLNSSVPSCPACTAAQTQRNCSNCQLQNKTCVQCAVKSAILPYSNCATCSRKSDSLSKHKRSESSASSSPSSGSGSGPGSSGTSGVSSVNGPGTPTNLTSGTAGCLVGLLKRHSNDSSGAVSISMAEREREREREMFKTYIATQGCLLTQQVNTVTDFWNMVWQENTRVIVMTTKEYERGKEKCARYWPDEGRSEQFGHARIQCVSENSTSDYTLREFLVSWRDQPARRIFHYHFQVWPDHGVPADPGCVLNFLQDVNTRQSHLAQAGEKPGPICVHCSAGIGRTGTFIVIDMILDQIVRNGLDTEIDIQRTIQMVRSQRSGLVQTEAQYKFVYYAVQHYIQTLIARKRAEEQSLQVGREYTNIKYTGEIGNDSQRSPLPPAISSISLVPSKTPLTPTSADLGTGMGLSMGVGMGVGNKHASKQQPPLPVVNCNNNNNGIGNSGCSNGGGSSTTSSSNGSSNGNINALLGGIGLGLGGNMRKSNFYSDSLKQQQQREEQAPAGAAKFKNIPKDMIGLRPPSHAPALPPPPTPPRKT

Target ORF information:

RefSeq Version	NM_057782.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster corkscrew (csw), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057782.5

ORF Insert Sequence:

ATGTCATCGC GAAGATGGTT CCACCCAACG ATATCTGGCA TCGAAGCTGA GAAACTGCTG 
CAGGAGCAGG GATTCGACGG CTCCTTCCTC GCCCGCCTCT CCTCCTCGAA TCCGGGCGCC 
TTCACGCTCT CCGTGCGCCG CGGCAACGAG GTGACCCACA TCAAAATCCA AAACAATGGC 
GACTTCTTTG ATCTCTACGG TGGTGAAAAG TTCGCCACAC TGCCGGAACT GGTACAATAC 
TACATGGAGA ATGGCGAGCT AAAGGAGAAG AACGGCCAGG CCATCGAACT CAAGCAGCCG 
CTGATCTGCG CCGAGCCCAC CACGGAAAGA TGGTTTCATG GCAATCTTTC CGGAAAGGAA 
GCGGAAAAAT TGATCCTGGA GCGGGGCAAG AATGGTTCGT TTCTCGTCCG TGAATCTCAG 
AGCAAGCCTG GCGACTTCGT CCTTTCCGTG CGCACGGACG ACAAAGTAAC GCATGTCATG 
ATTCGATGGC AGGACAAGAA GTACGACGTC GGCGGCGGGG AATCCTTTGG CACCTTGTCG 
GAACTGATCG ATCACTACAA GCGTAATCCC ATGGTGGAGA CGTGCGGAAC CGTGGTGCAT 
CTGCGACAGC CATTCAACGC CACACGAATC ACGGCGGCCG GCATCAATGC CCGGGTGGAA 
CAGCTGGTCA AGGGAGGTTT CTGGGAGGAA TTCGAATCGC TGCAACAGGA CAGTCGGGAC 
ACATTCTCGC GCAACGAGGG CTACAAACAG GAGAACCGCC TCAAGAATCG CTACCGCAAC 
ATATTGCCAT ACGACCACAC GCGCGTCAAG CTGCTGGACG TGGAGCATAG CGTGGCCGGA 
GCCGAGTACA TCAATGCCAA CTACATACGG CTGCCCACCG ACGGCGACCT GTACAACATG 
AGCAGCTCGT CGGAGAGCCT GAACAGCTCG GTGCCCTCGT GCCCCGCCTG CACGGCTGCC 
CAGACACAGC GGAACTGCTC CAACTGCCAG CTGCAAAACA AGACGTGCGT GCAGTGCGCC 
GTGAAGAGCG CCATTCTGCC GTATAGCAAC TGTGCCACCT GCAGCCGCAA GTCAGACTCC 
CTGAGCAAGC ACAAGCGGAG CGAATCCTCG GCCTCTTCAT CGCCCTCCTC CGGCTCTGGG 
TCCGGACCAG GATCGTCGGG CACCAGCGGA GTGAGCAGCG TCAATGGACC CGGCACACCC 
ACCAATCTCA CGAGCGGCAC AGCCGGATGT CTGGTCGGCC TGCTGAAGAG ACACTCGAAC 
GACTCGTCCG GAGCTGTTTC TATATCGATG GCCGAACGGG AACGCGAGAG GGAGCGCGAG 
ATGTTTAAGA CCTACATCGC CACCCAGGGC TGTCTGCTCA CCCAGCAAGT GAACACGGTG 
ACGGACTTCT GGAACATGGT CTGGCAGGAG AACACGCGGG TGATCGTCAT GACCACCAAG 
GAGTACGAGC GCGGCAAAGA AAAGTGCGCC CGCTACTGGC CGGACGAGGG TAGATCGGAG 
CAGTTCGGCC ACGCGCGGAT ACAGTGCGTC TCGGAGAACT CGACCAGTGA CTATACGCTG 
CGCGAGTTCC TCGTCTCGTG GCGGGATCAG CCGGCGCGCC GGATCTTTCA CTACCATTTC 
CAGGTGTGGC CGGATCACGG AGTGCCCGCC GATCCGGGCT GTGTGCTCAA CTTCCTGCAA 
GATGTCAACA CGCGTCAGAG TCACCTGGCT CAAGCGGGCG AGAAGCCGGG TCCGATCTGC 
GTGCACTGCT CTGCGGGCAT CGGTCGCACT GGCACCTTTA TTGTGATCGA TATGATTCTC 
GATCAGATTG TGCGCAATGG ATTGGATACT GAAATCGACA TCCAGCGCAC CATTCAGATG 
GTCCGATCGC AGCGTTCCGG TCTTGTGCAA ACCGAGGCGC AATACAAGTT CGTCTACTAT 
GCGGTGCAGC ACTATATACA GACCCTGATC GCCCGGAAAC GAGCTGAGGA GCAGAGCCTG 
CAGGTTGGCC GCGAGTACAC CAATATAAAG TACACGGGCG AAATTGGAAA CGATTCACAA 
AGATCTCCAT TACCACCAGC AATTTCTAGC ATAAGTTTAG TTCCGAGTAA GACGCCACTG 
ACGCCGACAT CGGCGGATTT GGGCACTGGG ATGGGCCTAA GCATGGGCGT GGGCATGGGC 
GTCGGCAACA AGCACGCATC GAAGCAGCAG CCGCCGTTGC CGGTGGTCAA CTGCAACAAT 
AATAACAACG GCATTGGCAA TAGCGGCTGC AGCAACGGCG GCGGGAGCAG CACCACCAGC 
AGCAGCAACG GCAGCAGCAA CGGTAACATC AACGCCCTAC TGGGCGGCAT CGGCTTGGGG 
CTGGGCGGCA ATATGCGCAA GTCGAACTTT TACAGCGACT CGCTGAAGCA GCAACAGCAG 
CGCGAGGAGC AGGCTCCGGC GGGAGCAGCA AAATTCAAAA ACATTCCCAA AGACATGATC 
GGCTTGCGAC CGCCAAGCCA TGCGCCTGCG TTGCCGCCGC CACCGACACC GCCGCGCAAA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

csw ( NM_057783.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477131.1 Drosophila melanogaster corkscrew (csw), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa02377
Clone ID Related Accession (Same CDS sequence)	NM_057783.3
Accession Version	NM_057783.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2838bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	corkscrew, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_057783.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGTTCA ACAAATGTCT GGAAAAGTTG TCCAGCTCGC TGGGCAATGT GGTCAATCAC 
AAGCTGCAAG AGAAACAAGT CTACAACAAC AACAATATCA ACAATAACAA TAACAATACG 
CTAAACAACA ACAATGCCTA CAACAATCAG CGAAACTTTG AGTACGAAAG AGCCATACAG 
GCGCACTACG GAAGCAAGGG AAGACGCTCG GAGGAGCGCG AAAGGAGCGG CAAGTTCAAG 
GCCAGCAAGG GTCGGAAAGC AAAGGTCACC CCACCAACGG AGACACCCGA GGCCCAGGAG 
CCGGCCTGCA AGAACTGTAT GACCCACGAC GAGCTGGCCC AGATCATAAA GGGCGTGGCC 
AAGGGCGCTG ACGCGCAACG TAATCGAGAC AACCGACTGC AGCGCAGACG TCGTCCTCTC 
TCCGCCCAAC CCTCCGCCGC TGCCTCCGCC TCCACATCGA CGGAATCTCT GCACCGTCTT 
ACACCCAGCC CGCAGGCTTC CTACCCGGCC ACGCCCACCT CCTGGACAGC CACACCGCCC 
CAGTTCCCAG CCGCCTTCGG CGGCGCCAGC TGCTCCAACA GCACACTGTC CCTCTTGGCC 
ACCATGCGCG TCCAGCTCCA TGGTTACACA TGGTTTCATG GCAATCTTTC CGGAAAGGAA 
GCGGAAAAAT TGATCCTGGA GCGGGGCAAG AATGGTTCGT TTCTCGTCCG TGAATCTCAG 
AGCAAGCCTG GCGACTTCGT CCTTTCCGTG CGCACGGACG ACAAAGTAAC GCATGTCATG 
ATTCGATGGC AGGACAAGAA GTACGACGTC GGCGGCGGGG AATCCTTTGG CACCTTGTCG 
GAACTGATCG ATCACTACAA GCGTAATCCC ATGGTGGAGA CGTGCGGAAC CGTGGTGCAT 
CTGCGACAGC CATTCAACGC CACACGAATC ACGGCGGCCG GCATCAATGC CCGGGTGGAA 
CAGCTGGTCA AGGGAGGTTT CTGGGAGGAA TTCGAATCGC TGCAACAGGA CAGTCGGGAC 
ACATTCTCGC GCAACGAGGG CTACAAACAG GAGAACCGCC TCAAGAATCG CTACCGCAAC 
ATATTGCCAT ACGACCACAC GCGCGTCAAG CTGCTGGACG TGGAGCATAG CGTGGCCGGA 
GCCGAGTACA TCAATGCCAA CTACATACGG CTGCCCACCG ACGGCGACCT GTACAACATG 
AGCAGCTCGT CGGAGAGCCT GAACAGCTCG GTGCCCTCGT GCCCCGCCTG CACGGCTGCC 
CAGACACAGC GGAACTGCTC CAACTGCCAG CTGCAAAACA AGACGTGCGT GCAGTGCGCC 
GTGAAGAGCG CCATTCTGCC GTATAGCAAC TGTGCCACCT GCAGCCGCAA GTCAGACTCC 
CTGAGCAAGC ACAAGCGGAG CGAATCCTCG GCCTCTTCAT CGCCCTCCTC CGGCTCTGGG 
TCCGGACCAG GATCGTCGGG CACCAGCGGA GTGAGCAGCG TCAATGGACC CGGCACACCC 
ACCAATCTCA CGAGCGGCAC AGCCGGATGT CTGGTCGGCC TGCTGAAGAG ACACTCGAAC 
GACTCGTCCG GAGCTGTTTC TATATCGATG GCCGAACGGG AACGCGAGAG GGAGCGCGAG 
ATGTTTAAGA CCTACATCGC CACCCAGGGC TGTCTGCTCA CCCAGCAAGT GAACACGGTG 
ACGGACTTCT GGAACATGGT CTGGCAGGAG AACACGCGGG TGATCGTCAT GACCACCAAG 
GAGTACGAGC GCGGCAAAGA AAAGTGCGCC CGCTACTGGC CGGACGAGGG TAGATCGGAG 
CAGTTCGGCC ACGCGCGGAT ACAGTGCGTC TCGGAGAACT CGACCAGTGA CTATACGCTG 
CGCGAGTTCC TCGTCTCGTG GCGGGATCAG CCGGCGCGCC GGATCTTTCA CTACCATTTC 
CAGGTGTGGC CGGATCACGG AGTGCCCGCC GATCCGGGCT GTGTGCTCAA CTTCCTGCAA 
GATGTCAACA CGCGTCAGAG TCACCTGGCT CAAGCGGGCG AGAAGCCGGG TCCGATCTGC 
GTGCACTGCT CTGCGGGCAT CGGTCGCACT GGCACCTTTA TTGTGATCGA TATGATTCTC 
GATCAGATTG TGCGCAATGG ATTGGATACT GAAATCGACA TCCAGCGCAC CATTCAGATG 
GTCCGATCGC AGCGTTCCGG TCTTGTGCAA ACCGAGGCGC AATACAAGTT CGTCTACTAT 
GCGGTGCAGC ACTATATACA GACCCTGATC GCCCGGAAAC GAGCTGAGGA GCAGAGCCTG 
CAGGTTGGCC GCGAGTACAC CAATATAAAG TACACGGGCG AAATTGGAAA CGATTCACAA 
AGATCTCCAT TACCACCAGC AATTTCTAGC ATAAGTTTAG TTCCGAGTAA GACGCCACTG 
ACGCCGACAT CGGCGGATTT GGGCACTGGG ATGGGCCTAA GCATGGGCGT GGGCATGGGC 
GTCGGCAACA AGCACGCATC GAAGCAGCAG CCGCCGTTGC CGGTGGTCAA CTGCAACAAT 
AATAACAACG GCATTGGCAA TAGCGGCTGC AGCAACGGCG GCGGGAGCAG CACCACCAGC 
AGCAGCAACG GCAGCAGCAA CGGTAACATC AACGCCCTAC TGGGCGGCAT CGGCTTGGGG 
CTGGGCGGCA ATATGCGCAA GTCGAACTTT TACAGCGACT CGCTGAAGCA GCAACAGCAG 
CGCGAGGAGC AGGCTCCGGC GGGAGCAGGT AAGATGCAGC AGCCGGCGCC GCCGCTGCGA 
CCGCGTCCTG GAATACTCAA GTTGCTCACC AGTCCCGTCA TCTTTCAGCA AAATTCAAAA 
ACATTCCCAA AGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477131.1
CDS	859..3696
Translation	MLFNKCLEKLSSSLGNVVNHKLQEKQVYNNNNINNNNNNTLNNNNAYNNQRNFEYERAIQAHYGSKGRRSEERERSGKFKASKGRKAKVTPPTETPEAQEPACKNCMTHDELAQIIKGVAKGADAQRNRDNRLQRRRRPLSAQPSAAASASTSTESLHRLTPSPQASYPATPTSWTATPPQFPAAFGGASCSNSTLSLLATMRVQLHGYTWFHGNLSGKEAEKLILERGKNGSFLVRESQSKPGDFVLSVRTDDKVTHVMIRWQDKKYDVGGGESFGTLSELIDHYKRNPMVETCGTVVHLRQPFNATRITAAGINARVEQLVKGGFWEEFESLQQDSRDTFSRNEGYKQENRLKNRYRNILPYDHTRVKLLDVEHSVAGAEYINANYIRLPTDGDLYNMSSSSESLNSSVPSCPACTAAQTQRNCSNCQLQNKTCVQCAVKSAILPYSNCATCSRKSDSLSKHKRSESSASSSPSSGSGSGPGSSGTSGVSSVNGPGTPTNLTSGTAGCLVGLLKRHSNDSSGAVSISMAEREREREREMFKTYIATQGCLLTQQVNTVTDFWNMVWQENTRVIVMTTKEYERGKEKCARYWPDEGRSEQFGHARIQCVSENSTSDYTLREFLVSWRDQPARRIFHYHFQVWPDHGVPADPGCVLNFLQDVNTRQSHLAQAGEKPGPICVHCSAGIGRTGTFIVIDMILDQIVRNGLDTEIDIQRTIQMVRSQRSGLVQTEAQYKFVYYAVQHYIQTLIARKRAEEQSLQVGREYTNIKYTGEIGNDSQRSPLPPAISSISLVPSKTPLTPTSADLGTGMGLSMGVGMGVGNKHASKQQPPLPVVNCNNNNNGIGNSGCSNGGGSSTTSSSNGSSNGNINALLGGIGLGLGGNMRKSNFYSDSLKQQQQREEQAPAGAGKMQQPAPPLRPRPGILKLLTSPVIFQQNSKTFPKT

Target ORF information:

RefSeq Version	NM_057783.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster corkscrew (csw), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057783.3

ORF Insert Sequence:

ATGCTGTTCA ACAAATGTCT GGAAAAGTTG TCCAGCTCGC TGGGCAATGT GGTCAATCAC 
AAGCTGCAAG AGAAACAAGT CTACAACAAC AACAATATCA ACAATAACAA TAACAATACG 
CTAAACAACA ACAATGCCTA CAACAATCAG CGAAACTTTG AGTACGAAAG AGCCATACAG 
GCGCACTACG GAAGCAAGGG AAGACGCTCG GAGGAGCGCG AAAGGAGCGG CAAGTTCAAG 
GCCAGCAAGG GTCGGAAAGC AAAGGTCACC CCACCAACGG AGACACCCGA GGCCCAGGAG 
CCGGCCTGCA AGAACTGTAT GACCCACGAC GAGCTGGCCC AGATCATAAA GGGCGTGGCC 
AAGGGCGCTG ACGCGCAACG TAATCGAGAC AACCGACTGC AGCGCAGACG TCGTCCTCTC 
TCCGCCCAAC CCTCCGCCGC TGCCTCCGCC TCCACATCGA CGGAATCTCT GCACCGTCTT 
ACACCCAGCC CGCAGGCTTC CTACCCGGCC ACGCCCACCT CCTGGACAGC CACACCGCCC 
CAGTTCCCAG CCGCCTTCGG CGGCGCCAGC TGCTCCAACA GCACACTGTC CCTCTTGGCC 
ACCATGCGCG TCCAGCTCCA TGGTTACACA TGGTTTCATG GCAATCTTTC CGGAAAGGAA 
GCGGAAAAAT TGATCCTGGA GCGGGGCAAG AATGGTTCGT TTCTCGTCCG TGAATCTCAG 
AGCAAGCCTG GCGACTTCGT CCTTTCCGTG CGCACGGACG ACAAAGTAAC GCATGTCATG 
ATTCGATGGC AGGACAAGAA GTACGACGTC GGCGGCGGGG AATCCTTTGG CACCTTGTCG 
GAACTGATCG ATCACTACAA GCGTAATCCC ATGGTGGAGA CGTGCGGAAC CGTGGTGCAT 
CTGCGACAGC CATTCAACGC CACACGAATC ACGGCGGCCG GCATCAATGC CCGGGTGGAA 
CAGCTGGTCA AGGGAGGTTT CTGGGAGGAA TTCGAATCGC TGCAACAGGA CAGTCGGGAC 
ACATTCTCGC GCAACGAGGG CTACAAACAG GAGAACCGCC TCAAGAATCG CTACCGCAAC 
ATATTGCCAT ACGACCACAC GCGCGTCAAG CTGCTGGACG TGGAGCATAG CGTGGCCGGA 
GCCGAGTACA TCAATGCCAA CTACATACGG CTGCCCACCG ACGGCGACCT GTACAACATG 
AGCAGCTCGT CGGAGAGCCT GAACAGCTCG GTGCCCTCGT GCCCCGCCTG CACGGCTGCC 
CAGACACAGC GGAACTGCTC CAACTGCCAG CTGCAAAACA AGACGTGCGT GCAGTGCGCC 
GTGAAGAGCG CCATTCTGCC GTATAGCAAC TGTGCCACCT GCAGCCGCAA GTCAGACTCC 
CTGAGCAAGC ACAAGCGGAG CGAATCCTCG GCCTCTTCAT CGCCCTCCTC CGGCTCTGGG 
TCCGGACCAG GATCGTCGGG CACCAGCGGA GTGAGCAGCG TCAATGGACC CGGCACACCC 
ACCAATCTCA CGAGCGGCAC AGCCGGATGT CTGGTCGGCC TGCTGAAGAG ACACTCGAAC 
GACTCGTCCG GAGCTGTTTC TATATCGATG GCCGAACGGG AACGCGAGAG GGAGCGCGAG 
ATGTTTAAGA CCTACATCGC CACCCAGGGC TGTCTGCTCA CCCAGCAAGT GAACACGGTG 
ACGGACTTCT GGAACATGGT CTGGCAGGAG AACACGCGGG TGATCGTCAT GACCACCAAG 
GAGTACGAGC GCGGCAAAGA AAAGTGCGCC CGCTACTGGC CGGACGAGGG TAGATCGGAG 
CAGTTCGGCC ACGCGCGGAT ACAGTGCGTC TCGGAGAACT CGACCAGTGA CTATACGCTG 
CGCGAGTTCC TCGTCTCGTG GCGGGATCAG CCGGCGCGCC GGATCTTTCA CTACCATTTC 
CAGGTGTGGC CGGATCACGG AGTGCCCGCC GATCCGGGCT GTGTGCTCAA CTTCCTGCAA 
GATGTCAACA CGCGTCAGAG TCACCTGGCT CAAGCGGGCG AGAAGCCGGG TCCGATCTGC 
GTGCACTGCT CTGCGGGCAT CGGTCGCACT GGCACCTTTA TTGTGATCGA TATGATTCTC 
GATCAGATTG TGCGCAATGG ATTGGATACT GAAATCGACA TCCAGCGCAC CATTCAGATG 
GTCCGATCGC AGCGTTCCGG TCTTGTGCAA ACCGAGGCGC AATACAAGTT CGTCTACTAT 
GCGGTGCAGC ACTATATACA GACCCTGATC GCCCGGAAAC GAGCTGAGGA GCAGAGCCTG 
CAGGTTGGCC GCGAGTACAC CAATATAAAG TACACGGGCG AAATTGGAAA CGATTCACAA 
AGATCTCCAT TACCACCAGC AATTTCTAGC ATAAGTTTAG TTCCGAGTAA GACGCCACTG 
ACGCCGACAT CGGCGGATTT GGGCACTGGG ATGGGCCTAA GCATGGGCGT GGGCATGGGC 
GTCGGCAACA AGCACGCATC GAAGCAGCAG CCGCCGTTGC CGGTGGTCAA CTGCAACAAT 
AATAACAACG GCATTGGCAA TAGCGGCTGC AGCAACGGCG GCGGGAGCAG CACCACCAGC 
AGCAGCAACG GCAGCAGCAA CGGTAACATC AACGCCCTAC TGGGCGGCAT CGGCTTGGGG 
CTGGGCGGCA ATATGCGCAA GTCGAACTTT TACAGCGACT CGCTGAAGCA GCAACAGCAG 
CGCGAGGAGC AGGCTCCGGC GGGAGCAGGT AAGATGCAGC AGCCGGCGCC GCCGCTGCGA 
CCGCGTCCTG GAATACTCAA GTTGCTCACC AGTCCCGTCA TCTTTCAGCA AAATTCAAAA 
ACATTCCCAA AGACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

csw ( NM_166928.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726793.1 Drosophila melanogaster corkscrew (csw), transcript variant C, mRNA.

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CloneID	OFa02378
Clone ID Related Accession (Same CDS sequence)	NM_166928.2
Accession Version	NM_166928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	corkscrew, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166928.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTCGAT GGCAGGACAA GAAGTACGAC GTCGGCGGCG GGGAATCCTT TGGCACCTTG 
TCGGAACTGA TCGATCACTA CAAGCGTAAT CCCATGGTGG AGACGTGCGG AACCGTGGTG 
CATCTGCGAC AGCCATTCAA CGCCACACGA ATCACGGCGG CCGGCATCAA TGCCCGGGTG 
GAACAGCTGG TCAAGGGAGG TTTCTGGGAG GAATTCGAAT CGCTGCAACA GGACAGTCGG 
GACACATTCT CGCGCAACGA GGGCTACAAA CAGGAGAACC GCCTCAAGAA TCGCTACCGC 
AACATATTGC CATACGACCA CACGCGCGTC AAGCTGCTGG ACGTGGAGCA TAGCGTGGCC 
GGAGCCGAGT ACATCAATGC CAACTACATA CGGCTGCCCA CCGACGGCGA CCTGTACAAC 
ATGAGCAGCT CGTCGGAGAG CCTGAACAGC TCGGTGCCCT CGTGCCCCGC CTGCACGGCT 
GCCCAGACAC AGCGGAACTG CTCCAACTGC CAGCTGCAAA ACAAGACGTG CGTGCAGTGC 
GCCGTGAAGA GCGCCATTCT GCCGTATAGC AACTGTGCCA CCTGCAGCCG CAAGTCAGAC 
TCCCTGAGCA AGCACAAGCG GAGCGAATCC TCGGCCTCTT CATCGCCCTC CTCCGGCTCT 
GGGTCCGGAC CAGGATCGTC GGGCACCAGC GGAGTGAGCA GCGTCAATGG ACCCGGCACA 
CCCACCAATC TCACGAGCGG CACAGCCGGA TGTCTGGTCG GCCTGCTGAA GAGACACTCG 
AACGACTCGT CCGGAGCTGT TTCTATATCG ATGGCCGAAC GGGAACGCGA GAGGGAGCGC 
GAGATGTTTA AGACCTACAT CGCCACCCAG GGCTGTCTGC TCACCCAGCA AGTGAACACG 
GTGACGGACT TCTGGAACAT GGTCTGGCAG GAGAACACGC GGGTGATCGT CATGACCACC 
AAGGAGTACG AGCGCGGCAA AGAAAAGTGC GCCCGCTACT GGCCGGACGA GGGTAGATCG 
GAGCAGTTCG GCCACGCGCG GATACAGTGC GTCTCGGAGA ACTCGACCAG TGACTATACG 
CTGCGCGAGT TCCTCGTCTC GTGGCGGGAT CAGCCGGCGC GCCGGATCTT TCACTACCAT 
TTCCAGGTGT GGCCGGATCA CGGAGTGCCC GCCGATCCGG GCTGTGTGCT CAACTTCCTG 
CAAGATGTCA ACACGCGTCA GAGTCACCTG GCTCAAGCGG GCGAGAAGCC GGGTCCGATC 
TGCGTGCACT GCTCTGCGGG CATCGGTCGC ACTGGCACCT TTATTGTGAT CGATATGATT 
CTCGATCAGA TTGTGCGCAA TGGATTGGAT ACTGAAATCG ACATCCAGCG CACCATTCAG 
ATGGTCCGAT CGCAGCGTTC CGGTCTTGTG CAAACCGAGG CGCAATACAA GTTCGTCTAC 
TATGCGGTGC AGCACTATAT ACAGACCCTG ATCGCCCGGA AACGAGCTGA GGAGCAGAGC 
CTGCAGGTTG GCCGCGAGTA CACCAATATA AAGTACACGG GCGAAATTGG AAACGATTCA 
CAAAGATCTC CATTACCACC AGCAATTTCT AGCATAAGTT TAGTTCCGAG TAAGACGCCA 
CTGACGCCGA CATCGGCGGA TTTGGGCACT GGGATGGGCC TAAGCATGGG CGTGGGCATG 
GGCGTCGGCA ACAAGCACGC ATCGAAGCAG CAGCCGCCGT TGCCGGTGGT CAACTGCAAC 
AATAATAACA ACGGCATTGG CAATAGCGGC TGCAGCAACG GCGGCGGGAG CAGCACCACC 
AGCAGCAGCA ACGGCAGCAG CAACGGTAAC ATCAACGCCC TACTGGGCGG CATCGGCTTG 
GGGCTGGGCG GCAATATGCG CAAGTCGAAC TTTTACAGCG ACTCGCTGAA GCAGCAACAG 
CAGCGCGAGG AGCAGGCTCC GGCGGGAGCA GCAAAATTCA AAAACATTCC CAAAGACATG 
ATCGGCTTGC GACCGCCAAG CCATGCGCCT GCGTTGCCGC CGCCACCGAC ACCGCCGCGC 
AAAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726793.1
CDS	195..2243
Translation	MIRWQDKKYDVGGGESFGTLSELIDHYKRNPMVETCGTVVHLRQPFNATRITAAGINARVEQLVKGGFWEEFESLQQDSRDTFSRNEGYKQENRLKNRYRNILPYDHTRVKLLDVEHSVAGAEYINANYIRLPTDGDLYNMSSSSESLNSSVPSCPACTAAQTQRNCSNCQLQNKTCVQCAVKSAILPYSNCATCSRKSDSLSKHKRSESSASSSPSSGSGSGPGSSGTSGVSSVNGPGTPTNLTSGTAGCLVGLLKRHSNDSSGAVSISMAEREREREREMFKTYIATQGCLLTQQVNTVTDFWNMVWQENTRVIVMTTKEYERGKEKCARYWPDEGRSEQFGHARIQCVSENSTSDYTLREFLVSWRDQPARRIFHYHFQVWPDHGVPADPGCVLNFLQDVNTRQSHLAQAGEKPGPICVHCSAGIGRTGTFIVIDMILDQIVRNGLDTEIDIQRTIQMVRSQRSGLVQTEAQYKFVYYAVQHYIQTLIARKRAEEQSLQVGREYTNIKYTGEIGNDSQRSPLPPAISSISLVPSKTPLTPTSADLGTGMGLSMGVGMGVGNKHASKQQPPLPVVNCNNNNNGIGNSGCSNGGGSSTTSSSNGSSNGNINALLGGIGLGLGGNMRKSNFYSDSLKQQQQREEQAPAGAAKFKNIPKDMIGLRPPSHAPALPPPPTPPRKT

Target ORF information:

RefSeq Version	NM_166928.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster corkscrew (csw), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166928.2

ORF Insert Sequence:

ATGATTCGAT GGCAGGACAA GAAGTACGAC GTCGGCGGCG GGGAATCCTT TGGCACCTTG 
TCGGAACTGA TCGATCACTA CAAGCGTAAT CCCATGGTGG AGACGTGCGG AACCGTGGTG 
CATCTGCGAC AGCCATTCAA CGCCACACGA ATCACGGCGG CCGGCATCAA TGCCCGGGTG 
GAACAGCTGG TCAAGGGAGG TTTCTGGGAG GAATTCGAAT CGCTGCAACA GGACAGTCGG 
GACACATTCT CGCGCAACGA GGGCTACAAA CAGGAGAACC GCCTCAAGAA TCGCTACCGC 
AACATATTGC CATACGACCA CACGCGCGTC AAGCTGCTGG ACGTGGAGCA TAGCGTGGCC 
GGAGCCGAGT ACATCAATGC CAACTACATA CGGCTGCCCA CCGACGGCGA CCTGTACAAC 
ATGAGCAGCT CGTCGGAGAG CCTGAACAGC TCGGTGCCCT CGTGCCCCGC CTGCACGGCT 
GCCCAGACAC AGCGGAACTG CTCCAACTGC CAGCTGCAAA ACAAGACGTG CGTGCAGTGC 
GCCGTGAAGA GCGCCATTCT GCCGTATAGC AACTGTGCCA CCTGCAGCCG CAAGTCAGAC 
TCCCTGAGCA AGCACAAGCG GAGCGAATCC TCGGCCTCTT CATCGCCCTC CTCCGGCTCT 
GGGTCCGGAC CAGGATCGTC GGGCACCAGC GGAGTGAGCA GCGTCAATGG ACCCGGCACA 
CCCACCAATC TCACGAGCGG CACAGCCGGA TGTCTGGTCG GCCTGCTGAA GAGACACTCG 
AACGACTCGT CCGGAGCTGT TTCTATATCG ATGGCCGAAC GGGAACGCGA GAGGGAGCGC 
GAGATGTTTA AGACCTACAT CGCCACCCAG GGCTGTCTGC TCACCCAGCA AGTGAACACG 
GTGACGGACT TCTGGAACAT GGTCTGGCAG GAGAACACGC GGGTGATCGT CATGACCACC 
AAGGAGTACG AGCGCGGCAA AGAAAAGTGC GCCCGCTACT GGCCGGACGA GGGTAGATCG 
GAGCAGTTCG GCCACGCGCG GATACAGTGC GTCTCGGAGA ACTCGACCAG TGACTATACG 
CTGCGCGAGT TCCTCGTCTC GTGGCGGGAT CAGCCGGCGC GCCGGATCTT TCACTACCAT 
TTCCAGGTGT GGCCGGATCA CGGAGTGCCC GCCGATCCGG GCTGTGTGCT CAACTTCCTG 
CAAGATGTCA ACACGCGTCA GAGTCACCTG GCTCAAGCGG GCGAGAAGCC GGGTCCGATC 
TGCGTGCACT GCTCTGCGGG CATCGGTCGC ACTGGCACCT TTATTGTGAT CGATATGATT 
CTCGATCAGA TTGTGCGCAA TGGATTGGAT ACTGAAATCG ACATCCAGCG CACCATTCAG 
ATGGTCCGAT CGCAGCGTTC CGGTCTTGTG CAAACCGAGG CGCAATACAA GTTCGTCTAC 
TATGCGGTGC AGCACTATAT ACAGACCCTG ATCGCCCGGA AACGAGCTGA GGAGCAGAGC 
CTGCAGGTTG GCCGCGAGTA CACCAATATA AAGTACACGG GCGAAATTGG AAACGATTCA 
CAAAGATCTC CATTACCACC AGCAATTTCT AGCATAAGTT TAGTTCCGAG TAAGACGCCA 
CTGACGCCGA CATCGGCGGA TTTGGGCACT GGGATGGGCC TAAGCATGGG CGTGGGCATG 
GGCGTCGGCA ACAAGCACGC ATCGAAGCAG CAGCCGCCGT TGCCGGTGGT CAACTGCAAC 
AATAATAACA ACGGCATTGG CAATAGCGGC TGCAGCAACG GCGGCGGGAG CAGCACCACC 
AGCAGCAGCA ACGGCAGCAG CAACGGTAAC ATCAACGCCC TACTGGGCGG CATCGGCTTG 
GGGCTGGGCG GCAATATGCG CAAGTCGAAC TTTTACAGCG ACTCGCTGAA GCAGCAACAG 
CAGCGCGAGG AGCAGGCTCC GGCGGGAGCA GCAAAATTCA AAAACATTCC CAAAGACATG 
ATCGGCTTGC GACCGCCAAG CCATGCGCCT GCGTTGCCGC CGCCACCGAC ACCGCCGCGC 
AAAACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

csw ( NM_001272245.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259174.1 Drosophila melanogaster corkscrew (csw), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03748
Clone ID Related Accession (Same CDS sequence)	NM_001272245.1
Accession Version	NM_001272245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2685bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	corkscrew, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACACAC CCCACTGTGC CCCACTGTTC GCCACTGTGC TCCACTGTGC GCCACTGCAC 
CTGCCACTGA TGACCACTTG GTATGCAGGG CAGGAGGCAC CGGAGGCTGA CCACAAACGA 
CATTCGCACA ATGGCCAGTG GCAGCTGACA ACACCCATAT GTTTGCCCAC CGGATGGTTC 
CACCCAACGA TATCTGGCAT CGAAGCTGAG AAACTGCTGC AGGAGCAGGG ATTCGACGGC 
TCCTTCCTCG CCCGCCTCTC CTCCTCGAAT CCGGGCGCCT TCACGCTCTC CGTGCGCCGC 
GGCAACGAGG TGACCCACAT CAAAATCCAA AACAATGGCG ACTTCTTTGA TCTCTACGGT 
GGTGAAAAGT TCGCCACACT GCCGGAACTG GTACAATACT ACATGGAGAA TGGCGAGCTA 
AAGGAGAAGA ACGGCCAGGC CATCGAACTC AAGCAGCCGC TGATCTGCGC CGAGCCCACC 
ACGGAAAGAT GGTTTCATGG CAATCTTTCC GGAAAGGAAG CGGAAAAATT GATCCTGGAG 
CGGGGCAAGA ATGGTTCGTT TCTCGTCCGT GAATCTCAGA GCAAGCCTGG CGACTTCGTC 
CTTTCCGTGC GCACGGACGA CAAAGTAACG CATGTCATGA TTCGATGGCA GGACAAGAAG 
TACGACGTCG GCGGCGGGGA ATCCTTTGGC ACCTTGTCGG AACTGATCGA TCACTACAAG 
CGTAATCCCA TGGTGGAGAC GTGCGGAACC GTGGTGCATC TGCGACAGCC ATTCAACGCC 
ACACGAATCA CGGCGGCCGG CATCAATGCC CGGGTGGAAC AGCTGGTCAA GGGAGGTTTC 
TGGGAGGAAT TCGAATCGCT GCAACAGGAC AGTCGGGACA CATTCTCGCG CAACGAGGGC 
TACAAACAGG AGAACCGCCT CAAGAATCGC TACCGCAACA TATTGCCATA CGACCACACG 
CGCGTCAAGC TGCTGGACGT GGAGCATAGC GTGGCCGGAG CCGAGTACAT CAATGCCAAC 
TACATACGGC TGCCCACCGA CGGCGACCTG TACAACATGA GCAGCTCGTC GGAGAGCCTG 
AACAGCTCGG TGCCCTCGTG CCCCGCCTGC ACGGCTGCCC AGACACAGCG GAACTGCTCC 
AACTGCCAGC TGCAAAACAA GACGTGCGTG CAGTGCGCCG TGAAGAGCGC CATTCTGCCG 
TATAGCAACT GTGCCACCTG CAGCCGCAAG TCAGACTCCC TGAGCAAGCA CAAGCGGAGC 
GAATCCTCGG CCTCTTCATC GCCCTCCTCC GGCTCTGGGT CCGGACCAGG ATCGTCGGGC 
ACCAGCGGAG TGAGCAGCGT CAATGGACCC GGCACACCCA CCAATCTCAC GAGCGGCACA 
GCCGGATGTC TGGTCGGCCT GCTGAAGAGA CACTCGAACG ACTCGTCCGG AGCTGTTTCT 
ATATCGATGG CCGAACGGGA ACGCGAGAGG GAGCGCGAGA TGTTTAAGAC CTACATCGCC 
ACCCAGGGCT GTCTGCTCAC CCAGCAAGTG AACACGGTGA CGGACTTCTG GAACATGGTC 
TGGCAGGAGA ACACGCGGGT GATCGTCATG ACCACCAAGG AGTACGAGCG CGGCAAAGAA 
AAGTGCGCCC GCTACTGGCC GGACGAGGGT AGATCGGAGC AGTTCGGCCA CGCGCGGATA 
CAGTGCGTCT CGGAGAACTC GACCAGTGAC TATACGCTGC GCGAGTTCCT CGTCTCGTGG 
CGGGATCAGC CGGCGCGCCG GATCTTTCAC TACCATTTCC AGGTGTGGCC GGATCACGGA 
GTGCCCGCCG ATCCGGGCTG TGTGCTCAAC TTCCTGCAAG ATGTCAACAC GCGTCAGAGT 
CACCTGGCTC AAGCGGGCGA GAAGCCGGGT CCGATCTGCG TGCACTGCTC TGCGGGCATC 
GGTCGCACTG GCACCTTTAT TGTGATCGAT ATGATTCTCG ATCAGATTGT GCGCAATGGA 
TTGGATACTG AAATCGACAT CCAGCGCACC ATTCAGATGG TCCGATCGCA GCGTTCCGGT 
CTTGTGCAAA CCGAGGCGCA ATACAAGTTC GTCTACTATG CGGTGCAGCA CTATATACAG 
ACCCTGATCG CCCGGAAACG AGCTGAGGAG CAGAGCCTGC AGGTTGGCCG CGAGTACACC 
AATATAAAGT ACACGGGCGA AATTGGAAAC GATTCACAAA GATCTCCATT ACCACCAGCA 
ATTTCTAGCA TAAGTTTAGT TCCGAGTAAG ACGCCACTGA CGCCGACATC GGCGGATTTG 
GGCACTGGGA TGGGCCTAAG CATGGGCGTG GGCATGGGCG TCGGCAACAA GCACGCATCG 
AAGCAGCAGC CGCCGTTGCC GGTGGTCAAC TGCAACAATA ATAACAACGG CATTGGCAAT 
AGCGGCTGCA GCAACGGCGG CGGGAGCAGC ACCACCAGCA GCAGCAACGG CAGCAGCAAC 
GGTAACATCA ACGCCCTACT GGGCGGCATC GGCTTGGGGC TGGGCGGCAA TATGCGCAAG 
TCGAACTTTT ACAGCGACTC GCTGAAGCAG CAACAGCAGC GCGAGGAGCA GGCTCCGGCG 
GGAGCAGCAA AATTCAAAAA CATTCCCAAA GACATGATCG GCTTGCGACC GCCAAGCCAT 
GCGCCTGCGT TGCCGCCGCC ACCGACACCG CCGCGCAAAA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259174.1
CDS	535..3219
Translation	MHTPHCAPLFATVLHCAPLHLPLMTTWYAGQEAPEADHKRHSHNGQWQLTTPICLPTGWFHPTISGIEAEKLLQEQGFDGSFLARLSSSNPGAFTLSVRRGNEVTHIKIQNNGDFFDLYGGEKFATLPELVQYYMENGELKEKNGQAIELKQPLICAEPTTERWFHGNLSGKEAEKLILERGKNGSFLVRESQSKPGDFVLSVRTDDKVTHVMIRWQDKKYDVGGGESFGTLSELIDHYKRNPMVETCGTVVHLRQPFNATRITAAGINARVEQLVKGGFWEEFESLQQDSRDTFSRNEGYKQENRLKNRYRNILPYDHTRVKLLDVEHSVAGAEYINANYIRLPTDGDLYNMSSSSESLNSSVPSCPACTAAQTQRNCSNCQLQNKTCVQCAVKSAILPYSNCATCSRKSDSLSKHKRSESSASSSPSSGSGSGPGSSGTSGVSSVNGPGTPTNLTSGTAGCLVGLLKRHSNDSSGAVSISMAEREREREREMFKTYIATQGCLLTQQVNTVTDFWNMVWQENTRVIVMTTKEYERGKEKCARYWPDEGRSEQFGHARIQCVSENSTSDYTLREFLVSWRDQPARRIFHYHFQVWPDHGVPADPGCVLNFLQDVNTRQSHLAQAGEKPGPICVHCSAGIGRTGTFIVIDMILDQIVRNGLDTEIDIQRTIQMVRSQRSGLVQTEAQYKFVYYAVQHYIQTLIARKRAEEQSLQVGREYTNIKYTGEIGNDSQRSPLPPAISSISLVPSKTPLTPTSADLGTGMGLSMGVGMGVGNKHASKQQPPLPVVNCNNNNNGIGNSGCSNGGGSSTTSSSNGSSNGNINALLGGIGLGLGGNMRKSNFYSDSLKQQQQREEQAPAGAAKFKNIPKDMIGLRPPSHAPALPPPPTPPRKT

Target ORF information:

RefSeq Version	NM_001272245.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster corkscrew (csw), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272245.1

ORF Insert Sequence:

ATGCACACAC CCCACTGTGC CCCACTGTTC GCCACTGTGC TCCACTGTGC GCCACTGCAC 
CTGCCACTGA TGACCACTTG GTATGCAGGG CAGGAGGCAC CGGAGGCTGA CCACAAACGA 
CATTCGCACA ATGGCCAGTG GCAGCTGACA ACACCCATAT GTTTGCCCAC CGGATGGTTC 
CACCCAACGA TATCTGGCAT CGAAGCTGAG AAACTGCTGC AGGAGCAGGG ATTCGACGGC 
TCCTTCCTCG CCCGCCTCTC CTCCTCGAAT CCGGGCGCCT TCACGCTCTC CGTGCGCCGC 
GGCAACGAGG TGACCCACAT CAAAATCCAA AACAATGGCG ACTTCTTTGA TCTCTACGGT 
GGTGAAAAGT TCGCCACACT GCCGGAACTG GTACAATACT ACATGGAGAA TGGCGAGCTA 
AAGGAGAAGA ACGGCCAGGC CATCGAACTC AAGCAGCCGC TGATCTGCGC CGAGCCCACC 
ACGGAAAGAT GGTTTCATGG CAATCTTTCC GGAAAGGAAG CGGAAAAATT GATCCTGGAG 
CGGGGCAAGA ATGGTTCGTT TCTCGTCCGT GAATCTCAGA GCAAGCCTGG CGACTTCGTC 
CTTTCCGTGC GCACGGACGA CAAAGTAACG CATGTCATGA TTCGATGGCA GGACAAGAAG 
TACGACGTCG GCGGCGGGGA ATCCTTTGGC ACCTTGTCGG AACTGATCGA TCACTACAAG 
CGTAATCCCA TGGTGGAGAC GTGCGGAACC GTGGTGCATC TGCGACAGCC ATTCAACGCC 
ACACGAATCA CGGCGGCCGG CATCAATGCC CGGGTGGAAC AGCTGGTCAA GGGAGGTTTC 
TGGGAGGAAT TCGAATCGCT GCAACAGGAC AGTCGGGACA CATTCTCGCG CAACGAGGGC 
TACAAACAGG AGAACCGCCT CAAGAATCGC TACCGCAACA TATTGCCATA CGACCACACG 
CGCGTCAAGC TGCTGGACGT GGAGCATAGC GTGGCCGGAG CCGAGTACAT CAATGCCAAC 
TACATACGGC TGCCCACCGA CGGCGACCTG TACAACATGA GCAGCTCGTC GGAGAGCCTG 
AACAGCTCGG TGCCCTCGTG CCCCGCCTGC ACGGCTGCCC AGACACAGCG GAACTGCTCC 
AACTGCCAGC TGCAAAACAA GACGTGCGTG CAGTGCGCCG TGAAGAGCGC CATTCTGCCG 
TATAGCAACT GTGCCACCTG CAGCCGCAAG TCAGACTCCC TGAGCAAGCA CAAGCGGAGC 
GAATCCTCGG CCTCTTCATC GCCCTCCTCC GGCTCTGGGT CCGGACCAGG ATCGTCGGGC 
ACCAGCGGAG TGAGCAGCGT CAATGGACCC GGCACACCCA CCAATCTCAC GAGCGGCACA 
GCCGGATGTC TGGTCGGCCT GCTGAAGAGA CACTCGAACG ACTCGTCCGG AGCTGTTTCT 
ATATCGATGG CCGAACGGGA ACGCGAGAGG GAGCGCGAGA TGTTTAAGAC CTACATCGCC 
ACCCAGGGCT GTCTGCTCAC CCAGCAAGTG AACACGGTGA CGGACTTCTG GAACATGGTC 
TGGCAGGAGA ACACGCGGGT GATCGTCATG ACCACCAAGG AGTACGAGCG CGGCAAAGAA 
AAGTGCGCCC GCTACTGGCC GGACGAGGGT AGATCGGAGC AGTTCGGCCA CGCGCGGATA 
CAGTGCGTCT CGGAGAACTC GACCAGTGAC TATACGCTGC GCGAGTTCCT CGTCTCGTGG 
CGGGATCAGC CGGCGCGCCG GATCTTTCAC TACCATTTCC AGGTGTGGCC GGATCACGGA 
GTGCCCGCCG ATCCGGGCTG TGTGCTCAAC TTCCTGCAAG ATGTCAACAC GCGTCAGAGT 
CACCTGGCTC AAGCGGGCGA GAAGCCGGGT CCGATCTGCG TGCACTGCTC TGCGGGCATC 
GGTCGCACTG GCACCTTTAT TGTGATCGAT ATGATTCTCG ATCAGATTGT GCGCAATGGA 
TTGGATACTG AAATCGACAT CCAGCGCACC ATTCAGATGG TCCGATCGCA GCGTTCCGGT 
CTTGTGCAAA CCGAGGCGCA ATACAAGTTC GTCTACTATG CGGTGCAGCA CTATATACAG 
ACCCTGATCG CCCGGAAACG AGCTGAGGAG CAGAGCCTGC AGGTTGGCCG CGAGTACACC 
AATATAAAGT ACACGGGCGA AATTGGAAAC GATTCACAAA GATCTCCATT ACCACCAGCA 
ATTTCTAGCA TAAGTTTAGT TCCGAGTAAG ACGCCACTGA CGCCGACATC GGCGGATTTG 
GGCACTGGGA TGGGCCTAAG CATGGGCGTG GGCATGGGCG TCGGCAACAA GCACGCATCG 
AAGCAGCAGC CGCCGTTGCC GGTGGTCAAC TGCAACAATA ATAACAACGG CATTGGCAAT 
AGCGGCTGCA GCAACGGCGG CGGGAGCAGC ACCACCAGCA GCAGCAACGG CAGCAGCAAC 
GGTAACATCA ACGCCCTACT GGGCGGCATC GGCTTGGGGC TGGGCGGCAA TATGCGCAAG 
TCGAACTTTT ACAGCGACTC GCTGAAGCAG CAACAGCAGC GCGAGGAGCA GGCTCCGGCG 
GGAGCAGCAA AATTCAAAAA CATTCCCAAA GACATGATCG GCTTGCGACC GCCAAGCCAT 
GCGCCTGCGT TGCCGCCGCC ACCGACACCG CCGCGCAAAA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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csw cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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