E(bx) gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_206224.3	NP_995946.1	enhancer of bithorax, isoform C
NM_001169833.2	NP_001163304.1	enhancer of bithorax, isoform E
NM_001169834.2	NP_001163305.1	enhancer of bithorax, isoform F
NM_001274258.1	NP_001261187.1	enhancer of bithorax, isoform G
NM_167819.3	NP_728507.1	enhancer of bithorax, isoform A
NM_001274260.1	NP_001261189.1	enhancer of bithorax, isoform I
NM_167817.3	NP_728505.1	enhancer of bithorax, isoform B
NM_001274259.1	NP_001261188.1	enhancer of bithorax, isoform H
NM_001274261.1	NP_001261190.1	enhancer of bithorax, isoform J

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19388	NM_001274258.1 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant G (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa19390	NM_001169834.2 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant F (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa20454	NM_206224.3 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant C (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa20490	NM_167817.3 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant B (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
OFa19386	NM_001274261.1 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant J (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa19386	NM_167819.3 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant A (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa19386	NM_001169833.2 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant E (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa19387	NM_001274260.1 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant I (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa19389	NM_001274259.1 Latest version!	Drosophila melanogaster enhancer of bithorax, transcript variant H (E(bx)), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

E(bx) ( NM_001274258.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261187.1 Drosophila melanogaster enhancer of bithorax, transcript variant G (E(bx)), mRNA.

Price & Availability↑

CloneID	OFa19388
Clone ID Related Accession (Same CDS sequence)	NM_001274258.1
Accession Version	NM_001274258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTTGC CTGGAGTAAA 
TTCTACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261187.1
CDS	226..6717
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQVAWSKFYN

Target ORF information:

RefSeq Version	NM_001274258.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant G (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274258.1

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTTGC CTGGAGTAAA 
TTCTACAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_001169834.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163305.1 Drosophila melanogaster enhancer of bithorax, transcript variant F (E(bx)), mRNA.

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CloneID	OFa19390
Clone ID Related Accession (Same CDS sequence)	NM_001169834.2
Accession Version	NM_001169834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169834.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTGAG TAGTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163305.1
CDS	226..6645
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQVSSF

Target ORF information:

RefSeq Version	NM_001169834.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant F (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169834.2

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTGAG TAGTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_206224.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995946.1 Drosophila melanogaster enhancer of bithorax, transcript variant C (E(bx)), mRNA.

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CloneID	OFa20454
Clone ID Related Accession (Same CDS sequence)	NM_206224.3
Accession Version	NM_206224.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206224.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTGAG TAGTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995946.1
CDS	638..7117
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQVSSF

Target ORF information:

RefSeq Version	NM_206224.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant C (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206224.3

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTGAG TAGTTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_167817.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728505.1 Drosophila melanogaster enhancer of bithorax, transcript variant B (E(bx)), mRNA.

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CloneID	OFa20490
Clone ID Related Accession (Same CDS sequence)	NM_167817.3
Accession Version	NM_167817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167817.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728505.1
CDS	638..8587
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Target ORF information:

RefSeq Version	NM_167817.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant B (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167817.3

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_001169833.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163304.1 Drosophila melanogaster enhancer of bithorax, transcript variant E (E(bx)), mRNA.

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CloneID	OFa19386
Clone ID Related Accession (Same CDS sequence)	NM_001169833.2 , NM_167819.3 , NM_001274261.1
Accession Version	NM_001169833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001169833.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163304.1
CDS	593..8602
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Target ORF information:

RefSeq Version	NM_001169833.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant E (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169833.2

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_167819.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728507.1 Drosophila melanogaster enhancer of bithorax, transcript variant A (E(bx)), mRNA.

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CloneID	OFa19386
Clone ID Related Accession (Same CDS sequence)	NM_001169833.2 , NM_167819.3 , NM_001274261.1
Accession Version	NM_167819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167819.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728507.1
CDS	638..8647
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Target ORF information:

RefSeq Version	NM_167819.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant A (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167819.3

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_001274261.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261190.1 Drosophila melanogaster enhancer of bithorax, transcript variant J (E(bx)), mRNA.

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CloneID	OFa19386
Clone ID Related Accession (Same CDS sequence)	NM_001169833.2 , NM_167819.3 , NM_001274261.1
Accession Version	NM_001274261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261190.1
CDS	135..8144
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Target ORF information:

RefSeq Version	NM_001274261.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant J (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274261.1

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAACCAA CAACATCGAA 
ATCGACGCCG ATACGCTAGC AACAACTTAC GAAGCCAATT CAACTATTAA AGATATTGCT 
ATTAATAATG GCGATGATCA GGAAAACAGT AAATGTGCAG AAACAGAAAA TTCTAATATT 
ACAACTAATG AGTCCTTTGC CGGTACATCT TCCCTATTAG AGGGTTCAGA ACATGATGAA 
CCAACTAACC TTGCCGGCCT CGATATATCA GAAACAGACT TAGAAAATAA ACAAAACGAA 
AGCTTCGTTG TTACCAGAGG ATACATACAG AAGTCTATTT CAAATGCTCT GAAACAAGGT 
AATTTAAGTC CGGAACTTGA AGAAAAACTT GTTTGTATGC AGAAGCAACA AGAAAATGCG 
AATTCGACGA ATGAGTGGGA AACATGCTCA AGGGGCAGTG TGAATGAGGA AGCACTAACT 
CCCAGTCGCC AAACTGATGA CACTGAATGG AAAATTCGTA CTTCCTTAAG ACGCCCAAAT 
GCTATGACAA CAAGCAGCCA ATTTAATCGA ATTTTAAAAA AAAATCGGAG CAAGAATGAC 
GAAGTCGCTG AACTTGGAGA GCAAAAGCAA TCGCAATTGG AACGTCATAA AGAATTGCTT 
AAAAAAAATA TTTTGCGCAA GCGTTCCTTA TTAGAGCGTA ATTTACAAAG CGAAATACAC 
GAAGATGTAA AAACTAAAGT CCAAAGACAT GTACGCCCCT TAAGTAACGC TTCACCGGAT 
GAACAAAGCG AAAATGAGAG AAGTGGCGAA CCAAATTTAG ATTTTAAACG GACAGAGGTG 
CAGAACCCCA GACATGGCGC AGGCCGACCA AAAAAGTTGA CAAGAAAAAA GGAAAAGTTG 
TATTGCATTT GCCGTACTCC ATATGATGAC ACAAAGTTTT ACGTGGGGTG CGACTTGTGC 
AGCAATTGGT TCCATGGGGA TTGTGTCAGT ATTACCGAGG AAGCTTCTAA AAAACTATCG 
GAGTTCATTT GCATTGATTG CAAGAGGGCG AGAGAAACGC AACAGCTTTA CTGTAGCTGT 
CGTCAACCAT ACGACGAGTC ACAATTTTAT ATATGCTGTG ATAAATGCCA GGATTGGTTT 
CATGGCCGTT GCGTTGGAAT ACTTCAGAGC GAAGCAGAGT TTATTGATGA ATATGTTTGC 
CCTGAATGCC AACGCAAAAA CGATGCTAAC GCTGCAAATA TGAAGAAACT GACTTCAAAT 
GACGTTGAAG AGCTGAAAAA CTTAATTAAG CAGATGCAGT TACACAAAAG TGCTTGGCCA 
TTTATGGAAC CGGTTGATCC GAAGGAAGCA CCCGATTATT ACAAAGTGAT TAAAGAGCCA 
ATGGATCTCA AACGGATGGA AATTAAACTC GAATCAAACA CCTATACCAA ACTATCTGAG 
TTTATAGGCG ATATGACAAA AATTTTTGAC AACTGCCGTT ACTATAACCC AAAAGAATCA 
TCCTTTTATA AGTGTGCTGA AGCACTAGAG TCATACTTTG TGCAAAAAAT TAAAAATTTT 
CGCGAAAATG TTTTTGACCA AAGAACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_001274260.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261189.1 Drosophila melanogaster enhancer of bithorax, transcript variant I (E(bx)), mRNA.

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CloneID	OFa19387
Clone ID Related Accession (Same CDS sequence)	NM_001274260.1
Accession Version	NM_001274260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GTAACATCCA TACGGTCCGG ACTGCGAAAA CGAAAGCGTG CTGAATCTCC TCAGCCAACC 
GAGCCACAAA TTACCGAGGA ATGGGTCGAC GAAGATAAAT TGGAGTTATG GGAAATTAAA 
TTTATGGGAG AAAAACAAGA AAAGGCACGC CTATCAGCTG TTACGCGATC TGTAGCATCC 
CGTCAATTGG AGGCAAGTGG TAGTAATGGT TCCAACACTT CAACTAATGG AGCTCTTGGC 
GTCGCCGGAC GCGTCCAACT GGCACCCAAG TTGAGTGAAG ATGTTAAAGA AAAAATGGAA 
CAACAATTAA AATTACAGCG CGCTGTTCAT CAGCAGAGAA AATTGGTTGC AACTGGCGAA 
ATAACCCGTT CTGTGACACC AGTTAAAGGC CAAGTTATCG GTAGCAGGCG TGTCATTGTT 
AAAAACCCTG ATGGCACAAC TCGAATTATT CAGCAGGCAG TAACACAGGT TTCAAGAACA 
GGAGGAGCTA ATACTGCAGC AGCAGCTGCT TCACCTACTG TAGGAGGCTC GACAAGCACT 
CAATCAAATC CATCCACATC TACACCGCAT AAAGTACAAA TTATAAGGGG CCCAGATGGC 
AAAGTAAGCG TACGCGGGTT GAATCCTGGG CAGCAGCTGG TTCAAATGCC GGATGGAAAA 
CTCCATGTGC TGACTACCAC AACATCTTCA AATTCGGCGG GGCAAGGAAA CAAAATGAAG 
GTTCCCATTA AGCCAGCTTC TACTTCGTCG TCACCGGCAA TCAGTTCAGC ACAGACTACT 
ACAAATCCAG TGACGCCAGT GATAAAGCAA ATTGCAGTTA AACATGTCAC GAAAAATTCT 
GCGACCCAAT CCATAGCATC ATCCTCGCGC GTAGCCTTAC CGCTTGCTCA AATTAAGAAT 
AAGTTGTTAC TGGCCCAACA GCAGCAGCAA TCAACATCTT CATCACCAGC AACTTCATCA 
TCCCCTGTGC AAAAGATAGT GTCGAAAGTG GTTAACACTA GTACCTCTGG GCAAACTCTA 
CAGCAAGTAT TCGTACAGTC TGGGTCAAAA CTGGTAGTGG GCCAAAATGC ACAAGGGCAA 
AAGGTTATTA TATCTACATC GGCGGCACAA CAGCAAGGAA CATCACCGGT TCAACAGCAG 
CAACTAGTAC AATCGCAACC AATTCAGCAG TCACCACAAC AGATCTCCAT GACACAGGTC 
GGTAATCAAC CAACGCAAAA AGTAATTCAG CAAATTGTTA ATACCAGCAA CGTGCAGCAG 
CAGATAGTAG TTGGCGGTCA ACGGATTATC TTAAGCCCCG GTCAAACTAT TGTTACTCAG 
AGGAATGTGC CGCAGAGTCA AGCATTGCAG ATGGTTCAGC AGCAGATTCA AACCCAGCAG 
CAGCAGCAAC AACATCATGT CGTCCAGCCT CAGCAACAAT TCGTTGTTCA ATCAAACCAA 
ATTGTACAAT CTTCGCCAAG TGCACAAACC AAACTGGTTA AACAGCTTGT GGTTCAACAA 
CAATCGCAGC AAACCATTGA AGAAAAAACG CAAATCACCA CGACTGATAG CAATGAAACT 
GGCACACAAC AAGTCTTGGT TCCAAATTCA ACTTTAGCCC AGCAGTTAGC ACAGGGCAAA 
TTGCAGGTGG CAACAGTGAA TGGGCAACAA GTAATCGTAA AACCGTTGGG AAATAATCAG 
GCTCAAATCG TGGCACATAT TAAACACCAA GGTGATGGGA ACGCTCACAT CGTTACAAGT 
AATTCAGCTA CTGCAGTACC TCAAGCTAAC CCGCAGACCT CACCAGTCAA GCAACAGGCT 
CTCCCACCAC AAAGTCCACA ACAAGTCGTT GTGCAGCAGC AGCAAATACA TCAACAGTCA 
CCCACAAACT TCGAAAGTGG CGTTACCCCA ATAACTCAGC AGCCGGTTCT CACCCAGGCT 
GTCCAGGCTC CAGCCCAACA GCAAGCCTTG AGTGTTGAGG AAAGTCTTCT GCAGAATCAG 
CCGCCTGGAA CAGTAATTAA ATGTGTCACC GCTCAAGTAT TGCAAACCGA GCATGGTCCA 
CGTATAGTAT TGCAAGGCCT GGTGGGCAAC GATTTCACTG CACAACAACT GCAATTGGTG 
CAAACGCAGG TGAAGCAGCA ACTAATGAAG GCTCAAGAGT CAAATGGCAA ACTTGGTGTT 
CTAGGCCCAA CAAAAATATA CTTGGCGGTA CAGCCGGAAA ATGCAGTTCA ATCACAGCCA 
CCTCCTCTAA CCCCTGTTCA CCAATCGGCT GCGCATCAGC AAACCAACAA CATCGAAATC 
GACGCCGATA CGCTAGCAAC AACTTACGAA GCCAATTCAA CTATTAAAGA TATTGCTATT 
AATAATGGCG ATGATCAGGA AAACAGTAAA TGTGCAGAAA CAGAAAATTC TAATATTACA 
ACTAATGAGT CCTTTGCCGG TACATCTTCC CTATTAGAGG GTTCAGAACA TGATGAACCA 
ACTAACCTTG CCGGCCTCGA TATATCAGAA ACAGACTTAG AAAATAAACA AAACGAAAGC 
TTCGTTGTTA CCAGAGGATA CATACAGAAG TCTATTTCAA ATGCTCTGAA ACAAGGTAAT 
TTAAGTCCGG AACTTGAAGA AAAACTTGTT TGTATGCAGA AGCAACAAGA AAATGCGAAT 
TCGACGAATG AGTGGGAAAC ATGCTCAAGG GGCAGTGTGA ATGAGGAAGC ACTAACTCCC 
AGTCGCCAAA CTGATGACAC TGAATGGAAA ATTCGTACTT CCTTAAGACG CCCAAATGCT 
ATGACAACAA GCAGCCAATT TAATCGAATT TTAAAAAAAA ATCGGAGCAA GAATGACGAA 
GTCGCTGAAC TTGGAGAGCA AAAGCAATCG CAATTGGAAC GTCATAAAGA ATTGCTTAAA 
AAAAATATTT TGCGCAAGCG TTCCTTATTA GAGCGTAATT TACAAAGCGA AATACACGAA 
GATGTAAAAA CTAAAGTCCA AAGACATGTA CGCCCCTTAA GTAACGCTTC ACCGGATGAA 
CAAAGCGAAA ATGAGAGAAG TGGCGAACCA AATTTAGATT TTAAACGGAC AGAGGTGCAG 
AACCCCAGAC ATGGCGCAGG CCGACCAAAA AAGTTGACAA GAAAAAAGGA AAAGTTGTAT 
TGCATTTGCC GTACTCCATA TGATGACACA AAGTTTTACG TGGGGTGCGA CTTGTGCAGC 
AATTGGTTCC ATGGGGATTG TGTCAGTATT ACCGAGGAAG CTTCTAAAAA ACTATCGGAG 
TTCATTTGCA TTGATTGCAA GAGGGCGAGA GAAACGCAAC AGCTTTACTG TAGCTGTCGT 
CAACCATACG ACGAGTCACA ATTTTATATA TGCTGTGATA AATGCCAGGA TTGGTTTCAT 
GGCCGTTGCG TTGGAATACT TCAGAGCGAA GCAGAGTTTA TTGATGAATA TGTTTGCCCT 
GAATGCCAAC GCAAAAACGA TGCTAACGCT GCAAATATGA AGAAACTGAC TTCAAATGAC 
GTTGAAGAGC TGAAAAACTT AATTAAGCAG ATGCAGTTAC ACAAAAGTGC TTGGCCATTT 
ATGGAACCGG TTGATCCGAA GGAAGCACCC GATTATTACA AAGTGATTAA AGAGCCAATG 
GATCTCAAAC GGATGGAAAT TAAACTCGAA TCAAACACCT ATACCAAACT ATCTGAGTTT 
ATAGGCGATA TGACAAAAAT TTTTGACAAC TGCCGTTACT ATAACCCAAA AGAATCATCC 
TTTTATAAGT GTGCTGAAGC ACTAGAGTCA TACTTTGTGC AAAAAATTAA AAATTTTCGC 
GAAAATGTTT TTGACCAAAG AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261189.1
CDS	226..8232
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVRVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Target ORF information:

RefSeq Version	NM_001274260.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant I (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274260.1

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GTAACATCCA TACGGTCCGG ACTGCGAAAA CGAAAGCGTG CTGAATCTCC TCAGCCAACC 
GAGCCACAAA TTACCGAGGA ATGGGTCGAC GAAGATAAAT TGGAGTTATG GGAAATTAAA 
TTTATGGGAG AAAAACAAGA AAAGGCACGC CTATCAGCTG TTACGCGATC TGTAGCATCC 
CGTCAATTGG AGGCAAGTGG TAGTAATGGT TCCAACACTT CAACTAATGG AGCTCTTGGC 
GTCGCCGGAC GCGTCCAACT GGCACCCAAG TTGAGTGAAG ATGTTAAAGA AAAAATGGAA 
CAACAATTAA AATTACAGCG CGCTGTTCAT CAGCAGAGAA AATTGGTTGC AACTGGCGAA 
ATAACCCGTT CTGTGACACC AGTTAAAGGC CAAGTTATCG GTAGCAGGCG TGTCATTGTT 
AAAAACCCTG ATGGCACAAC TCGAATTATT CAGCAGGCAG TAACACAGGT TTCAAGAACA 
GGAGGAGCTA ATACTGCAGC AGCAGCTGCT TCACCTACTG TAGGAGGCTC GACAAGCACT 
CAATCAAATC CATCCACATC TACACCGCAT AAAGTACAAA TTATAAGGGG CCCAGATGGC 
AAAGTAAGCG TACGCGGGTT GAATCCTGGG CAGCAGCTGG TTCAAATGCC GGATGGAAAA 
CTCCATGTGC TGACTACCAC AACATCTTCA AATTCGGCGG GGCAAGGAAA CAAAATGAAG 
GTTCCCATTA AGCCAGCTTC TACTTCGTCG TCACCGGCAA TCAGTTCAGC ACAGACTACT 
ACAAATCCAG TGACGCCAGT GATAAAGCAA ATTGCAGTTA AACATGTCAC GAAAAATTCT 
GCGACCCAAT CCATAGCATC ATCCTCGCGC GTAGCCTTAC CGCTTGCTCA AATTAAGAAT 
AAGTTGTTAC TGGCCCAACA GCAGCAGCAA TCAACATCTT CATCACCAGC AACTTCATCA 
TCCCCTGTGC AAAAGATAGT GTCGAAAGTG GTTAACACTA GTACCTCTGG GCAAACTCTA 
CAGCAAGTAT TCGTACAGTC TGGGTCAAAA CTGGTAGTGG GCCAAAATGC ACAAGGGCAA 
AAGGTTATTA TATCTACATC GGCGGCACAA CAGCAAGGAA CATCACCGGT TCAACAGCAG 
CAACTAGTAC AATCGCAACC AATTCAGCAG TCACCACAAC AGATCTCCAT GACACAGGTC 
GGTAATCAAC CAACGCAAAA AGTAATTCAG CAAATTGTTA ATACCAGCAA CGTGCAGCAG 
CAGATAGTAG TTGGCGGTCA ACGGATTATC TTAAGCCCCG GTCAAACTAT TGTTACTCAG 
AGGAATGTGC CGCAGAGTCA AGCATTGCAG ATGGTTCAGC AGCAGATTCA AACCCAGCAG 
CAGCAGCAAC AACATCATGT CGTCCAGCCT CAGCAACAAT TCGTTGTTCA ATCAAACCAA 
ATTGTACAAT CTTCGCCAAG TGCACAAACC AAACTGGTTA AACAGCTTGT GGTTCAACAA 
CAATCGCAGC AAACCATTGA AGAAAAAACG CAAATCACCA CGACTGATAG CAATGAAACT 
GGCACACAAC AAGTCTTGGT TCCAAATTCA ACTTTAGCCC AGCAGTTAGC ACAGGGCAAA 
TTGCAGGTGG CAACAGTGAA TGGGCAACAA GTAATCGTAA AACCGTTGGG AAATAATCAG 
GCTCAAATCG TGGCACATAT TAAACACCAA GGTGATGGGA ACGCTCACAT CGTTACAAGT 
AATTCAGCTA CTGCAGTACC TCAAGCTAAC CCGCAGACCT CACCAGTCAA GCAACAGGCT 
CTCCCACCAC AAAGTCCACA ACAAGTCGTT GTGCAGCAGC AGCAAATACA TCAACAGTCA 
CCCACAAACT TCGAAAGTGG CGTTACCCCA ATAACTCAGC AGCCGGTTCT CACCCAGGCT 
GTCCAGGCTC CAGCCCAACA GCAAGCCTTG AGTGTTGAGG AAAGTCTTCT GCAGAATCAG 
CCGCCTGGAA CAGTAATTAA ATGTGTCACC GCTCAAGTAT TGCAAACCGA GCATGGTCCA 
CGTATAGTAT TGCAAGGCCT GGTGGGCAAC GATTTCACTG CACAACAACT GCAATTGGTG 
CAAACGCAGG TGAAGCAGCA ACTAATGAAG GCTCAAGAGT CAAATGGCAA ACTTGGTGTT 
CTAGGCCCAA CAAAAATATA CTTGGCGGTA CAGCCGGAAA ATGCAGTTCA ATCACAGCCA 
CCTCCTCTAA CCCCTGTTCA CCAATCGGCT GCGCATCAGC AAACCAACAA CATCGAAATC 
GACGCCGATA CGCTAGCAAC AACTTACGAA GCCAATTCAA CTATTAAAGA TATTGCTATT 
AATAATGGCG ATGATCAGGA AAACAGTAAA TGTGCAGAAA CAGAAAATTC TAATATTACA 
ACTAATGAGT CCTTTGCCGG TACATCTTCC CTATTAGAGG GTTCAGAACA TGATGAACCA 
ACTAACCTTG CCGGCCTCGA TATATCAGAA ACAGACTTAG AAAATAAACA AAACGAAAGC 
TTCGTTGTTA CCAGAGGATA CATACAGAAG TCTATTTCAA ATGCTCTGAA ACAAGGTAAT 
TTAAGTCCGG AACTTGAAGA AAAACTTGTT TGTATGCAGA AGCAACAAGA AAATGCGAAT 
TCGACGAATG AGTGGGAAAC ATGCTCAAGG GGCAGTGTGA ATGAGGAAGC ACTAACTCCC 
AGTCGCCAAA CTGATGACAC TGAATGGAAA ATTCGTACTT CCTTAAGACG CCCAAATGCT 
ATGACAACAA GCAGCCAATT TAATCGAATT TTAAAAAAAA ATCGGAGCAA GAATGACGAA 
GTCGCTGAAC TTGGAGAGCA AAAGCAATCG CAATTGGAAC GTCATAAAGA ATTGCTTAAA 
AAAAATATTT TGCGCAAGCG TTCCTTATTA GAGCGTAATT TACAAAGCGA AATACACGAA 
GATGTAAAAA CTAAAGTCCA AAGACATGTA CGCCCCTTAA GTAACGCTTC ACCGGATGAA 
CAAAGCGAAA ATGAGAGAAG TGGCGAACCA AATTTAGATT TTAAACGGAC AGAGGTGCAG 
AACCCCAGAC ATGGCGCAGG CCGACCAAAA AAGTTGACAA GAAAAAAGGA AAAGTTGTAT 
TGCATTTGCC GTACTCCATA TGATGACACA AAGTTTTACG TGGGGTGCGA CTTGTGCAGC 
AATTGGTTCC ATGGGGATTG TGTCAGTATT ACCGAGGAAG CTTCTAAAAA ACTATCGGAG 
TTCATTTGCA TTGATTGCAA GAGGGCGAGA GAAACGCAAC AGCTTTACTG TAGCTGTCGT 
CAACCATACG ACGAGTCACA ATTTTATATA TGCTGTGATA AATGCCAGGA TTGGTTTCAT 
GGCCGTTGCG TTGGAATACT TCAGAGCGAA GCAGAGTTTA TTGATGAATA TGTTTGCCCT 
GAATGCCAAC GCAAAAACGA TGCTAACGCT GCAAATATGA AGAAACTGAC TTCAAATGAC 
GTTGAAGAGC TGAAAAACTT AATTAAGCAG ATGCAGTTAC ACAAAAGTGC TTGGCCATTT 
ATGGAACCGG TTGATCCGAA GGAAGCACCC GATTATTACA AAGTGATTAA AGAGCCAATG 
GATCTCAAAC GGATGGAAAT TAAACTCGAA TCAAACACCT ATACCAAACT ATCTGAGTTT 
ATAGGCGATA TGACAAAAAT TTTTGACAAC TGCCGTTACT ATAACCCAAA AGAATCATCC 
TTTTATAAGT GTGCTGAAGC ACTAGAGTCA TACTTTGTGC AAAAAATTAA AAATTTTCGC 
GAAAATGTTT TTGACCAAAG AACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E(bx) ( NM_001274259.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261188.1 Drosophila melanogaster enhancer of bithorax, transcript variant H (E(bx)), mRNA.

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CloneID	OFa19389
Clone ID Related Accession (Same CDS sequence)	NM_001274259.1
Accession Version	NM_001274259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	enhancer of bithorax, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTTGG TGGCGGCGTA 
TCAATAGCTT ATACTCGTAT GATGAATTCG AATTATTTTG TGCAAGAAAT TGAAGAAGAA 
GAAGATTATG ATGATGAGAT TATTGTCGTC TGCAGTCCGC CGAATCAAAT TGTACCTTCA 
CCTCCCCGTG AAGTATTCGA AGGTAGAATT TTAAGAAGTG AATTGCCGTG CCGCACCAAC 
AATAATTTTG AAGATCAAGA CAATATTGAG CAGCATCGCC TAAAATATGA AAGTGAAGAC 
CTTAAACGAT GTTCATTGCA GACCAACAAC ATCGAAATCG ACGCCGATAC GCTAGCAACA 
ACTTACGAAG CCAATTCAAC TATTAAAGAT ATTGCTATTA ATAATGGCGA TGATCAGGAA 
AACAGTAAAT GTGCAGAAAC AGAAAATTCT AATATTACAA CTAATGAGTC CTTTGCCGGT 
ACATCTTCCC TATTAGAGGG TTCAGAACAT GATGAACCAA CTAACCTTGC CGGCCTCGAT 
ATATCAGAAA CAGACTTAGA AAATAAACAA AACGAAAGCT TCGTTGTTAC CAGAGGATAC 
ATACAGAAGT CTATTTCAAA TGCTCTGAAA CAAGGTAATT TAAGTCCGGA ACTTGAAGAA 
AAACTTGTTT GTATGCAGAA GCAACAAGAA AATGCGAATT CGACGAATGA GTGGGAAACA 
TGCTCAAGGG GCAGTGTGAA TGAGGAAGCA CTAACTCCCA GTCGCCAAAC TGATGACACT 
GAATGGAAAA TTCGTACTTC CTTAAGACGC CCAAATGCTA TGACAACAAG CAGCCAATTT 
AATCGAATTT TAAAAAAAAA TCGGAGCAAG AATGACGAAG TCGCTGAACT TGGAGAGCAA 
AAGCAATCGC AATTGGAACG TCATAAAGAA TTGCTTAAAA AAAATATTTT GCGCAAGCGT 
TCCTTATTAG AGCGTAATTT ACAAAGCGAA ATACACGAAG ATGTAAAAAC TAAAGTCCAA 
AGACATGTAC GCCCCTTAAG TAACGCTTCA CCGGATGAAC AAAGCGAAAA TGAGAGAAGT 
GGCGAACCAA ATTTAGATTT TAAACGGACA GAGGTGCAGA ACCCCAGACA TGGCGCAGGC 
CGACCAAAAA AGTTGACAAG AAAAAAGGAA AAGTTGTATT GCATTTGCCG TACTCCATAT 
GATGACACAA AGTTTTACGT GGGGTGCGAC TTGTGCAGCA ATTGGTTCCA TGGGGATTGT 
GTCAGTATTA CCGAGGAAGC TTCTAAAAAA CTATCGGAGT TCATTTGCAT TGATTGCAAG 
AGGGCGAGAG AAACGCAACA GCTTTACTGT AGCTGTCGTC AACCATACGA CGAGTCACAA 
TTTTATATAT GCTGTGATAA ATGCCAGGAT TGGTTTCATG GCCGTTGCGT TGGAATACTT 
CAGAGCGAAG CAGAGTTTAT TGATGAATAT GTTTGCCCTG AATGCCAACG CAAAAACGAT 
GCTAACGCTG CAAATATGAA GAAACTGACT TCAAATGACG TTGAAGAGCT GAAAAACTTA 
ATTAAGCAGA TGCAGTTACA CAAAAGTGCT TGGCCATTTA TGGAACCGGT TGATCCGAAG 
GAAGCACCCG ATTATTACAA AGTGATTAAA GAGCCAATGG ATCTCAAACG GATGGAAATT 
AAACTCGAAT CAAACACCTA TACCAAACTA TCTGAGTTTA TAGGCGATAT GACAAAAATT 
TTTGACAACT GCCGTTACTA TAACCCAAAA GAATCATCCT TTTATAAGTG TGCTGAAGCA 
CTAGAGTCAT ACTTTGTGCA AAAAATTAAA AATTTTCGCG AAAATGTTTT TGACCAAAGA 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261188.1
CDS	638..8923
Translation	MSGRGSRKRGRPPKTPNERASGRFNYQLLKKPKYLSEGKSQPSTPSASRGISPQSDEGSRSSHNNHTNRSRGSAAKRGRGRKSAVQPNTSSYSGRKGYESEYHYGSDFGDSEEDKSDNEDDMLLTPSDDESLEVANESESEFSVCSFNQNGVGRPPRPPSPEPVWLQEGRQYAALDLPDSSEDLFIANTHVLRALSIYEVLRRFRHMVRLSPFRFEDLCAALACEEQSALLTEVHIMLLKAILREEDAQGTHFGPLDQKDTVNISLYLIDSITWPEVLRSYVESDKTFDRNVFHILSHTEYPYTGIDNRLEVLQFLSDQFLTSNSIRDVMLQEGPIHYDDHCRVCHRLGDLLCCETCPAVYHLECVDPPMNDVPTEDWQCGLCRSHKVSGVVDCVLPQEKQGVLIRHDSLGVDRHGRKYWFIARRIFIEDQENFTCWYYSTTSKLKLLLSRLDAEELETRLHSQITERRDEIERQMKLTETLTNEHKHTKRSVIEIEQEAKNELLEKEVLDEDEKDGDAKSESQSIEGTKKQEECKMVTRQKSNQLTNGTLHFKLGMEQGFKNYVNQYSTNPIALNKPQRNEERDKRRHLSHKFSLTTASDFKWIGITMGTTDNMITTLRQTLINFESNIAASFLNINWVVNKKIWNAAVMNARRPSEFAVVLLLFQASLKSVVFANVWHEQLGHTTLQRITSAEREERKKLEKREKRERDDEEERNRLAFNYIKYTLGLKHQVWKQKGEEYRVHGQWGWLWLSSSRRCGVRARRAQPLTHNRVYVHYTMGEENDVNEIILVDPRTQRFMQQCESSNVDGQVCHYLPDQYKNVKVIEDVTEKIKGHIDVSKALNAPGRTYYSKVARKSRLDDLLDRRLKLAEVEEQMASKIPSDMKPLLVSSQNNTANSKQTFLEKRLLRLTEVQAKGGPANVNLELVNSLAKQIQTVRLQFSQLNRFAKVFRCYTKECNTNSNAVSQITQNTCYSPLCLQKARAKKELLLLLRKAHTAGNGSKETVAAILGAVKKPSILEQKLTEGKRESTQVAVDDSEEGKPAESEAPLDLLQDWEHARAHAVPFSDSLLTECILVDQECVTNTKIKQEVNASSGCNTTPDSNTQDSDKIDYIESMDVCSNVEIESTEDSIVTGLNSGNAEDVDMTPGWRRKRNQKSKKSYIGTKDVLDQTLDKDIPLNKQNRRFPITARPVKRECVKKYERETFENGNERVYSTSSPRGRVYLLNDAAKLYEQAVKTEDKSTITKKPSYSRYPLISNFLTHKKKRSLLVLPRFELLKLARLGGKSSTNGFHHAAKNNTIWQYQCSRPLFRTCWSYRTSNATSLSSLALQLRILWSCLRWDDMIAKPPSTDGKHQVTTDTEIVTLELLKLRHSGRYGEKTSYLRRKVVIPLEMPKTVREVTSIRSGLRKRKRAESPQPTEPQITEEWVDEDKLELWEIKFMGEKQEKARLSAVTRSVASRQLEASGSNGSNTSTNGALGVAGRVQLAPKLSEDVKEKMEQQLKLQRAVHQQRKLVATGEITRSVTPVKGQVIGSRRVIVKNPDGTTRIIQQAVTQVSRTGGANTAAAAASPTVGGSTSTQSNPSTSTPHKVQIIRGPDGKVSVRGLNPGQQLVQMPDGKLHVLTTTTSSNSAGQGNKMKVPIKPASTSSSPAISSAQTTTNPVTPVIKQIAVKHVTKNSATQSIASSSRVALPLAQIKNKLLLAQQQQQSTSSSPATSSSPVQKIVSKVVNTSTSGQTLQQVFVQSGSKLVVGQNAQGQKVIISTSAAQQQGTSPVQQQQLVQSQPIQQSPQQISMTQVGNQPTQKVIQQIVNTSNVQQQIVVGGQRIILSPGQTIVTQRNVPQSQALQMVQQQIQTQQQQQQHHVVQPQQQFVVQSNQIVQSSPSAQTKLVKQLVVQQQSQQTIEEKTQITTTDSNETGTQQVLVPNSTLAQQLAQGKLQVATVNGQQVIVKPLGNNQAQIVAHIKHQGDGNAHIVTSNSATAVPQANPQTSPVKQQALPPQSPQQVVVQQQQIHQQSPTNFESGVTPITQQPVLTQAVQAPAQQQALSVEESLLQNQPPGTVIKCVTAQVLQTEHGPRIVLQGLVGNDFTAQQLQLVQTQVKQQLMKAQESNGKLGVLGPTKIYLAVQPENAVQSQPPPLTPVHQSAAHQQVGGGVSIAYTRMMNSNYFVQEIEEEEDYDDEIIVVCSPPNQIVPSPPREVFEGRILRSELPCRTNNNFEDQDNIEQHRLKYESEDLKRCSLQTNNIEIDADTLATTYEANSTIKDIAINNGDDQENSKCAETENSNITTNESFAGTSSLLEGSEHDEPTNLAGLDISETDLENKQNESFVVTRGYIQKSISNALKQGNLSPELEEKLVCMQKQQENANSTNEWETCSRGSVNEEALTPSRQTDDTEWKIRTSLRRPNAMTTSSQFNRILKKNRSKNDEVAELGEQKQSQLERHKELLKKNILRKRSLLERNLQSEIHEDVKTKVQRHVRPLSNASPDEQSENERSGEPNLDFKRTEVQNPRHGAGRPKKLTRKKEKLYCICRTPYDDTKFYVGCDLCSNWFHGDCVSITEEASKKLSEFICIDCKRARETQQLYCSCRQPYDESQFYICCDKCQDWFHGRCVGILQSEAEFIDEYVCPECQRKNDANAANMKKLTSNDVEELKNLIKQMQLHKSAWPFMEPVDPKEAPDYYKVIKEPMDLKRMEIKLESNTYTKLSEFIGDMTKIFDNCRYYNPKESSFYKCAEALESYFVQKIKNFRENVFDQRT

Target ORF information:

RefSeq Version	NM_001274259.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster enhancer of bithorax, transcript variant H (E(bx)), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274259.1

ORF Insert Sequence:

ATGAGCGGTC GCGGCAGCCG TAAACGTGGA CGTCCGCCAA AGACGCCCAA CGAACGCGCA 
TCAGGACGCT TCAATTACCA GCTTCTCAAG AAGCCGAAGT ATCTCAGCGA GGGAAAGTCG 
CAGCCCAGCA CTCCGTCGGC ATCCAGGGGC ATTTCTCCAC AGAGCGACGA GGGCAGCAGG 
AGCAGCCACA ACAACCACAC CAATCGCAGT CGCGGGAGCG CCGCTAAGAG GGGACGAGGC 
CGCAAATCCG CCGTGCAACC GAACACCAGT AGCTACTCTG GAAGGAAAGG GTACGAGTCG 
GAGTATCACT ACGGGTCAGA TTTTGGGGAT TCCGAAGAAG ATAAATCCGA TAATGAGGAT 
GATATGCTGC TTACTCCCAG CGACGACGAG AGCCTAGAAG TAGCCAACGA GAGCGAATCA 
GAGTTTTCCG TGTGCAGCTT TAACCAAAAT GGAGTTGGTC GGCCGCCACG TCCTCCTAGC 
CCAGAACCTG TGTGGCTGCA GGAAGGTCGT CAGTATGCAG CGCTTGATTT GCCGGACTCG 
TCGGAGGATC TTTTCATTGC TAATACACAC GTTTTGCGCG CGCTAAGTAT CTATGAAGTA 
CTGCGACGAT TTCGCCACAT GGTCCGCCTT TCACCATTCC GATTTGAAGA CTTGTGTGCA 
GCGCTGGCTT GTGAGGAGCA AAGTGCATTA TTGACGGAGG TACACATAAT GCTGCTTAAA 
GCGATTCTTC GAGAAGAGGA CGCGCAGGGT ACACATTTTG GCCCGCTGGA TCAAAAAGAT 
ACGGTTAATA TAAGTCTTTA CCTGATTGAC TCTATAACGT GGCCGGAAGT TTTGCGAAGC 
TATGTAGAAA GCGATAAGAC ATTTGATCGC AACGTGTTTC ACATTTTAAG CCACACTGAA 
TACCCTTACA CGGGCATTGA CAACCGGCTT GAAGTGCTCC AGTTTCTGTC AGATCAATTT 
TTAACTTCCA ATTCTATACG AGATGTAATG CTGCAGGAGG GACCTATTCA TTATGATGAC 
CATTGCCGAG TGTGCCATCG GCTCGGTGAT TTATTATGTT GCGAGACGTG CCCTGCTGTC 
TATCATTTGG AATGTGTAGA CCCGCCAATG AATGACGTAC CTACTGAGGA TTGGCAGTGC 
GGGCTTTGCC GCTCGCATAA GGTAAGTGGC GTAGTGGACT GCGTGTTGCC ACAAGAAAAA 
CAAGGCGTGC TAATCCGGCA TGATAGCCTC GGTGTTGATC GTCATGGGCG CAAATATTGG 
TTTATTGCAC GTCGCATTTT TATCGAGGAT CAGGAGAACT TTACATGCTG GTATTACAGT 
ACGACAAGTA AGTTAAAATT GCTGCTCAGT CGACTGGACG CCGAAGAACT GGAAACTCGT 
CTGCATAGCC AGATTACAGA AAGGAGAGAC GAAATCGAGC GCCAAATGAA ACTGACTGAG 
ACCTTAACCA ATGAACACAA ACACACGAAG CGTAGTGTTA TCGAAATCGA ACAAGAGGCT 
AAAAATGAAC TTTTGGAGAA AGAGGTGCTT GACGAGGATG AAAAAGATGG TGATGCTAAA 
TCTGAGAGTC AATCTATAGA GGGAACAAAA AAACAAGAAG AATGTAAAAT GGTCACGCGT 
CAAAAATCCA ATCAGCTGAC TAATGGTACT TTGCATTTTA AACTTGGCAT GGAGCAAGGA 
TTTAAGAACT ATGTCAACCA GTATTCAACA AATCCAATTG CACTCAACAA GCCGCAACGA 
AACGAGGAGC GAGATAAGCG ACGCCATCTG TCCCATAAGT TCTCGTTAAC GACGGCTTCT 
GACTTTAAAT GGATCGGTAT AACCATGGGA ACTACTGACA ATATGATAAC AACTCTAAGG 
CAAACATTGA TAAACTTCGA ATCCAATATA GCAGCTTCCT TTTTAAATAT TAACTGGGTA 
GTTAACAAAA AAATTTGGAA TGCTGCGGTG ATGAACGCCC GTCGACCTTC TGAATTTGCG 
GTAGTATTGT TACTTTTTCA AGCGTCCCTT AAGAGCGTTG TTTTTGCCAA CGTCTGGCAT 
GAGCAACTCG GACACACAAC TTTGCAACGC ATAACTAGTG CCGAACGAGA GGAGCGAAAA 
AAACTAGAAA AGCGAGAGAA GCGCGAGCGG GATGATGAGG AAGAACGTAA TCGCTTAGCA 
TTTAACTACA TAAAGTATAC CCTGGGTCTC AAACATCAAG TCTGGAAGCA AAAAGGAGAA 
GAATACCGGG TCCACGGGCA ATGGGGCTGG CTTTGGCTCT CAAGCAGTCG ACGCTGTGGT 
GTTCGTGCAC GTCGAGCCCA ACCTCTAACA CACAATAGAG TCTACGTACA TTACACCATG 
GGAGAGGAAA ACGACGTAAA TGAAATTATT TTAGTCGACC CACGCACCCA ACGGTTTATG 
CAACAATGTG AGTCAAGCAA TGTCGATGGT CAGGTGTGTC ATTATTTACC GGACCAATAT 
AAAAATGTGA AAGTTATAGA AGATGTTACG GAGAAAATCA AAGGGCACAT CGACGTCAGC 
AAAGCGCTAA ATGCTCCAGG CCGTACTTAT TATTCAAAAG TGGCTCGCAA ATCTAGATTA 
GATGACTTAT TGGATCGCCG TTTAAAGTTG GCCGAGGTCG AGGAGCAGAT GGCTTCTAAA 
ATTCCTTCTG ATATGAAGCC GCTTCTTGTT TCTTCCCAAA ATAATACAGC CAACAGCAAA 
CAAACGTTTC TAGAGAAGCG TTTACTTCGC CTTACTGAAG TCCAAGCAAA GGGGGGTCCT 
GCAAACGTAA ACTTGGAACT TGTTAATTCG CTGGCTAAAC AAATTCAAAC CGTGCGCTTA 
CAATTTAGTC AGCTTAATCG ATTTGCTAAA GTTTTTCGTT GTTATACAAA GGAGTGTAAC 
ACCAATTCAA ATGCTGTCTC TCAAATAACC CAAAATACTT GCTACTCTCC CTTATGTCTT 
CAAAAAGCTC GGGCCAAAAA AGAACTTCTT TTGTTGTTAC GAAAGGCGCA TACTGCCGGT 
AATGGCTCAA AAGAGACAGT TGCCGCTATT TTGGGCGCTG TTAAAAAGCC ATCTATTCTC 
GAGCAGAAGC TGACGGAGGG TAAAAGAGAA TCCACTCAGG TGGCCGTGGA TGATTCTGAA 
GAGGGGAAGC CAGCTGAATC CGAAGCGCCG TTGGATTTAC TTCAAGATTG GGAACATGCA 
CGAGCGCACG CCGTTCCTTT TAGCGATTCT TTATTGACCG AATGCATCCT GGTTGACCAG 
GAGTGCGTAA CCAACACGAA AATTAAACAA GAGGTGAATG CCAGTTCCGG ATGCAATACA 
ACTCCTGACT CGAACACACA AGACTCCGAT AAAATAGACT ATATTGAGAG TATGGACGTT 
TGTAGTAATG TTGAAATTGA GAGTACCGAA GATTCCATTG TTACGGGCTT AAACTCTGGT 
AACGCTGAAG ATGTCGATAT GACACCTGGA TGGCGGCGAA AGCGTAACCA GAAATCGAAA 
AAAAGCTACA TCGGCACTAA AGATGTGTTG GATCAGACGT TAGACAAGGA TATACCACTT 
AACAAACAGA ACCGCAGATT TCCTATTACT GCACGTCCGG TTAAGCGCGA ATGTGTCAAA 
AAATACGAAC GAGAGACATT TGAGAATGGA AACGAGCGGG TCTATTCGAC TAGTTCACCT 
CGGGGTCGTG TATACCTCCT TAATGATGCG GCCAAGTTGT ATGAGCAAGC TGTCAAAACT 
GAGGATAAGT CAACGATAAC TAAAAAGCCA TCATACTCTC GGTATCCACT TATATCCAAT 
TTTCTTACTC ATAAAAAAAA GCGTAGCCTT TTAGTGCTAC CACGATTTGA GCTCCTGAAG 
CTGGCTCGTC TGGGTGGAAA ATCATCGACA AATGGTTTTC ATCACGCTGC GAAAAATAAC 
ACAATTTGGC AGTATCAGTG CTCGCGTCCC CTCTTCCGAA CCTGCTGGTC CTACAGAACA 
TCTAATGCCA CGTCCTTATC CAGCCTAGCA CTTCAGCTTC GAATATTGTG GTCGTGTCTC 
CGCTGGGATG ACATGATAGC AAAGCCCCCA TCCACGGATG GGAAGCATCA GGTTACTACG 
GATACTGAAA TTGTAACATT GGAATTGCTC AAACTTCGGC ACTCAGGACG CTATGGTGAA 
AAAACAAGCT ATTTGCGACG AAAAGTTGTT ATTCCACTTG AAATGCCGAA GACTGTAAGA 
GAAGTAACAT CCATACGGTC CGGACTGCGA AAACGAAAGC GTGCTGAATC TCCTCAGCCA 
ACCGAGCCAC AAATTACCGA GGAATGGGTC GACGAAGATA AATTGGAGTT ATGGGAAATT 
AAATTTATGG GAGAAAAACA AGAAAAGGCA CGCCTATCAG CTGTTACGCG ATCTGTAGCA 
TCCCGTCAAT TGGAGGCAAG TGGTAGTAAT GGTTCCAACA CTTCAACTAA TGGAGCTCTT 
GGCGTCGCCG GACGCGTCCA ACTGGCACCC AAGTTGAGTG AAGATGTTAA AGAAAAAATG 
GAACAACAAT TAAAATTACA GCGCGCTGTT CATCAGCAGA GAAAATTGGT TGCAACTGGC 
GAAATAACCC GTTCTGTGAC ACCAGTTAAA GGCCAAGTTA TCGGTAGCAG GCGTGTCATT 
GTTAAAAACC CTGATGGCAC AACTCGAATT ATTCAGCAGG CAGTAACACA GGTTTCAAGA 
ACAGGAGGAG CTAATACTGC AGCAGCAGCT GCTTCACCTA CTGTAGGAGG CTCGACAAGC 
ACTCAATCAA ATCCATCCAC ATCTACACCG CATAAAGTAC AAATTATAAG GGGCCCAGAT 
GGCAAAGTAA GCGTACGCGG GTTGAATCCT GGGCAGCAGC TGGTTCAAAT GCCGGATGGA 
AAACTCCATG TGCTGACTAC CACAACATCT TCAAATTCGG CGGGGCAAGG AAACAAAATG 
AAGGTTCCCA TTAAGCCAGC TTCTACTTCG TCGTCACCGG CAATCAGTTC AGCACAGACT 
ACTACAAATC CAGTGACGCC AGTGATAAAG CAAATTGCAG TTAAACATGT CACGAAAAAT 
TCTGCGACCC AATCCATAGC ATCATCCTCG CGCGTAGCCT TACCGCTTGC TCAAATTAAG 
AATAAGTTGT TACTGGCCCA ACAGCAGCAG CAATCAACAT CTTCATCACC AGCAACTTCA 
TCATCCCCTG TGCAAAAGAT AGTGTCGAAA GTGGTTAACA CTAGTACCTC TGGGCAAACT 
CTACAGCAAG TATTCGTACA GTCTGGGTCA AAACTGGTAG TGGGCCAAAA TGCACAAGGG 
CAAAAGGTTA TTATATCTAC ATCGGCGGCA CAACAGCAAG GAACATCACC GGTTCAACAG 
CAGCAACTAG TACAATCGCA ACCAATTCAG CAGTCACCAC AACAGATCTC CATGACACAG 
GTCGGTAATC AACCAACGCA AAAAGTAATT CAGCAAATTG TTAATACCAG CAACGTGCAG 
CAGCAGATAG TAGTTGGCGG TCAACGGATT ATCTTAAGCC CCGGTCAAAC TATTGTTACT 
CAGAGGAATG TGCCGCAGAG TCAAGCATTG CAGATGGTTC AGCAGCAGAT TCAAACCCAG 
CAGCAGCAGC AACAACATCA TGTCGTCCAG CCTCAGCAAC AATTCGTTGT TCAATCAAAC 
CAAATTGTAC AATCTTCGCC AAGTGCACAA ACCAAACTGG TTAAACAGCT TGTGGTTCAA 
CAACAATCGC AGCAAACCAT TGAAGAAAAA ACGCAAATCA CCACGACTGA TAGCAATGAA 
ACTGGCACAC AACAAGTCTT GGTTCCAAAT TCAACTTTAG CCCAGCAGTT AGCACAGGGC 
AAATTGCAGG TGGCAACAGT GAATGGGCAA CAAGTAATCG TAAAACCGTT GGGAAATAAT 
CAGGCTCAAA TCGTGGCACA TATTAAACAC CAAGGTGATG GGAACGCTCA CATCGTTACA 
AGTAATTCAG CTACTGCAGT ACCTCAAGCT AACCCGCAGA CCTCACCAGT CAAGCAACAG 
GCTCTCCCAC CACAAAGTCC ACAACAAGTC GTTGTGCAGC AGCAGCAAAT ACATCAACAG 
TCACCCACAA ACTTCGAAAG TGGCGTTACC CCAATAACTC AGCAGCCGGT TCTCACCCAG 
GCTGTCCAGG CTCCAGCCCA ACAGCAAGCC TTGAGTGTTG AGGAAAGTCT TCTGCAGAAT 
CAGCCGCCTG GAACAGTAAT TAAATGTGTC ACCGCTCAAG TATTGCAAAC CGAGCATGGT 
CCACGTATAG TATTGCAAGG CCTGGTGGGC AACGATTTCA CTGCACAACA ACTGCAATTG 
GTGCAAACGC AGGTGAAGCA GCAACTAATG AAGGCTCAAG AGTCAAATGG CAAACTTGGT 
GTTCTAGGCC CAACAAAAAT ATACTTGGCG GTACAGCCGG AAAATGCAGT TCAATCACAG 
CCACCTCCTC TAACCCCTGT TCACCAATCG GCTGCGCATC AGCAAGTTGG TGGCGGCGTA 
TCAATAGCTT ATACTCGTAT GATGAATTCG AATTATTTTG TGCAAGAAAT TGAAGAAGAA 
GAAGATTATG ATGATGAGAT TATTGTCGTC TGCAGTCCGC CGAATCAAAT TGTACCTTCA 
CCTCCCCGTG AAGTATTCGA AGGTAGAATT TTAAGAAGTG AATTGCCGTG CCGCACCAAC 
AATAATTTTG AAGATCAAGA CAATATTGAG CAGCATCGCC TAAAATATGA AAGTGAAGAC 
CTTAAACGAT GTTCATTGCA GACCAACAAC ATCGAAATCG ACGCCGATAC GCTAGCAACA 
ACTTACGAAG CCAATTCAAC TATTAAAGAT ATTGCTATTA ATAATGGCGA TGATCAGGAA 
AACAGTAAAT GTGCAGAAAC AGAAAATTCT AATATTACAA CTAATGAGTC CTTTGCCGGT 
ACATCTTCCC TATTAGAGGG TTCAGAACAT GATGAACCAA CTAACCTTGC CGGCCTCGAT 
ATATCAGAAA CAGACTTAGA AAATAAACAA AACGAAAGCT TCGTTGTTAC CAGAGGATAC 
ATACAGAAGT CTATTTCAAA TGCTCTGAAA CAAGGTAATT TAAGTCCGGA ACTTGAAGAA 
AAACTTGTTT GTATGCAGAA GCAACAAGAA AATGCGAATT CGACGAATGA GTGGGAAACA 
TGCTCAAGGG GCAGTGTGAA TGAGGAAGCA CTAACTCCCA GTCGCCAAAC TGATGACACT 
GAATGGAAAA TTCGTACTTC CTTAAGACGC CCAAATGCTA TGACAACAAG CAGCCAATTT 
AATCGAATTT TAAAAAAAAA TCGGAGCAAG AATGACGAAG TCGCTGAACT TGGAGAGCAA 
AAGCAATCGC AATTGGAACG TCATAAAGAA TTGCTTAAAA AAAATATTTT GCGCAAGCGT 
TCCTTATTAG AGCGTAATTT ACAAAGCGAA ATACACGAAG ATGTAAAAAC TAAAGTCCAA 
AGACATGTAC GCCCCTTAAG TAACGCTTCA CCGGATGAAC AAAGCGAAAA TGAGAGAAGT 
GGCGAACCAA ATTTAGATTT TAAACGGACA GAGGTGCAGA ACCCCAGACA TGGCGCAGGC 
CGACCAAAAA AGTTGACAAG AAAAAAGGAA AAGTTGTATT GCATTTGCCG TACTCCATAT 
GATGACACAA AGTTTTACGT GGGGTGCGAC TTGTGCAGCA ATTGGTTCCA TGGGGATTGT 
GTCAGTATTA CCGAGGAAGC TTCTAAAAAA CTATCGGAGT TCATTTGCAT TGATTGCAAG 
AGGGCGAGAG AAACGCAACA GCTTTACTGT AGCTGTCGTC AACCATACGA CGAGTCACAA 
TTTTATATAT GCTGTGATAA ATGCCAGGAT TGGTTTCATG GCCGTTGCGT TGGAATACTT 
CAGAGCGAAG CAGAGTTTAT TGATGAATAT GTTTGCCCTG AATGCCAACG CAAAAACGAT 
GCTAACGCTG CAAATATGAA GAAACTGACT TCAAATGACG TTGAAGAGCT GAAAAACTTA 
ATTAAGCAGA TGCAGTTACA CAAAAGTGCT TGGCCATTTA TGGAACCGGT TGATCCGAAG 
GAAGCACCCG ATTATTACAA AGTGATTAAA GAGCCAATGG ATCTCAAACG GATGGAAATT 
AAACTCGAAT CAAACACCTA TACCAAACTA TCTGAGTTTA TAGGCGATAT GACAAAAATT 
TTTGACAACT GCCGTTACTA TAACCCAAAA GAATCATCCT TTTATAAGTG TGCTGAAGCA 
CTAGAGTCAT ACTTTGTGCA AAAAATTAAA AATTTTCGCG AAAATGTTTT TGACCAAAGA 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	E(bx)
Entrez Gene ID	44811
Full Name	Enhancer of bithorax
Synonyms	CG10894,CG17135,CG32346,CG32478,CG7022,Dmel\CG32346,E(BX),E(Bx),Ebx,En-bx,NURF,NURF 215,NURF-215,NURF-301,NURF215,NURF301,NuRF301,Nurf 301,Nurf-215,Nurf301,dNURF,dNURF301,e(bx),l(3)122,l(3)ry122,nurf301,p215,p301
General protein information	Preferred Names Enhancer of bithorax Names CG32346 gene product from transcript CG32346-RJ CG32346-PA CG32346-PB CG32346-PC CG32346-PE CG32346-PF CG32346-PG CG32346-PH CG32346-PI CG32346-PJ E(bx)-PA E(bx)-PB E(bx)-PC E(bx)-PE E(bx)-PF E(bx)-PG E(bx)-PH E(bx)-PI E(bx)-PJ enhancer of bithorax nucleosome remodeling factor nucleosome remodeling factor - 215kD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61B2-61B3

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006133	XP_316196.4
Drosophila melanogaster (fruit fly)	E(bx)	NP_001261188.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

E(bx) cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

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