cwo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	cwo
Entrez Gene ID	44669
Full Name	clockwork orange
Synonyms	AAF24476.1,CG17100,CWO,Cwo,Dmel\CG17100,GM05287,bHLHe49,mel_cwo
General protein information	Preferred Names clockwork orange Names CG17100 gene product from transcript CG17100-RC CG17100-PA CG17100-PB CG17100-PC CLOCKWORK ORANGE clockworkorange cwo-PA cwo-PB cwo-PC stich
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86B3-86B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260095.2	NP_001247024.1	clockwork orange, isoform B
NM_001260096.2	NP_001247025.1	clockwork orange, isoform C
NM_080036.3	NP_524775.1	clockwork orange, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here we demonstrate that the transcription factor CLOCKWORK ORANGE (CWO) antagonizes CLK-CYC E-box binding, thus enhancing the removal of CLK-CYC from E-boxes to maintain transcriptional repression. This process requires PER, which suggests that PER-TIM and CWO cooperate to maintain a transcriptionally repressed state by removing CLK-CYC from E-boxes
Title: CLOCKWORK ORANGE Enhances PERIOD Mediated Rhythms in Transcriptional Repression by Antagonizing E-box Binding by CLOCK-CYCLE.

Results indicate that, in vivo, clockwork orange (CWO) modulates clock gene expression through both repressor and activator transcriptional functions.
Title: The clockwork orange Drosophila protein functions as both an activator and a repressor of clock gene expression.

findings suggest that Clockwork Orange (CWO) acts preferentially in the late night to help terminate CLK-CYC-mediated transcription of direct target genes including cwo itself
Title: Clockwork Orange is a transcriptional repressor and a new Drosophila circadian pacemaker component.

identification of novel clock gene candidates including clockwork orange (cwo), a transcriptional repressor belonging to the basic helix-loop-helix ORANGE family[
Title: A functional genomics strategy reveals clockwork orange as a transcriptional regulator in the Drosophila circadian clock.

The following cwo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cwo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08100	NM_001260095.2 Latest version!	Drosophila melanogaster clockwork orange (cwo), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08100	NM_001260096.2 Latest version!	Drosophila melanogaster clockwork orange (cwo), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa06859	NM_080036.3 Latest version!	Drosophila melanogaster clockwork orange (cwo), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cwo ( NM_001260095.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247024.1 Drosophila melanogaster clockwork orange (cwo), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08100
Clone ID Related Accession (Same CDS sequence)	NM_001260095.2 , NM_001260096.2
Accession Version	NM_001260095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clockwork orange, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260095.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCT GCCTGGCGGA CCTGTCCCGC CTCATCCCGC CGCAGTACCA GCGCAAGGGG 
CGAGGCCGTA TCGAGAAGAC GGAGATCATC GAGATGGCCA TCAGGCACCT GAAGCACCTG 
CAGAGCGAGT GCCAGCAGAA GGAGAGCGAC TACCGGAGTG GCTACATGGA CTGTATGAAG 
GAGGCGGCCA AGTTCCTCTA CGACGTTCAC ATGCAGGACT TTTGCCACCG ACTATTGGGT 
CGCCTGCAGG AGCACATCGA TGAGATGTTC AAAACGGACT GCTACAAGTC GACGCGCAGC 
TGCCACATGC CGGATAATGT AAGCGCCTCC AGCGGCAGTC CCCATCAGGC ATACCACCCA 
CCGCTGTGCC ACCTGCGTGA CATGTTGGCC ACCTCCGCCT CGGATGTGGA GCACAGTCAG 
GACCATAACG ACGTCAAGGA TCTGAGTTTC CGGAACCATC TCAACCAGCT TCAGAGGAGT 
CAGCAGGCGG CGGCAGCCGC AGCAGTAGCG GCAGCTGCCG TTGCAGTCGC CAATGGCAGT 
TCGCCGGCTT CGAATGCCGG TGTGGATTCC AAGGTCCCGC TGACCAATGG TGGCGGCACT 
GGTGGAGCAC CGCCGGCAGC TGATAATGTG CCCAGTAATT CCACGGGCAG TGGATCAGCT 
GCCGCGTGTG CTGGCGGAAA CAGCAACAGC AGCGGCAGTA ACAGCAGCAA TGCAGCCAGT 
TCCACCATCT GTCCACCGGC CGGAGGTAGC TGTCCAGCCA AGGTCACACC ACTGGCGGCA 
CACCAGCAGC CCCACCAGGC ACCAGTGATC ACTTCGACGG CCCCACATCA TCATCACCAC 
CATACGGACA GCAGCCACCA CGACTTCGAG TCGTCCAGGG AGCCCATCCT TCACACCGAT 
ACCTCTAACA TGCACTCGCC ACCGCCCAGG GATCTGCTGC TGCAGCAGCA TCCCCACCTG 
GCCCATAGCC ACCACACCCA GGATAGTCTG ATGTCCGTGA GGATGCGCAA CTATTCGGAG 
TCCTCGCACG AGATCGAGCA CAACAACAAC TACAAGTACA AGAACCACAT CAAGGAGCGG 
TTTGTCCACG AGCTCCACGA CGAGGAGACG AGCAGCGAGC ATTGCCCGGT CGCGGCCCAC 
CTCCAGAGCG ATCACTCCCA CTTGCAGGCC TTGTCGGAGC ACTCAAAGGA CGGCACTGAA 
CCCGAGATAG CACCAATTAT GGCCAAGAAG CGGAAGTTGG CCGAGGCAGC GGCCAATGGA 
GAAATACCCC TAGAGGTTCA CACCGAGTCC AGCAACGCGG GAGCGAGTTC CGCCAACCGG 
CTGGACAAGC CCTCGCCCTC GTTCAACTTC AGCGACATCA AGGACATCAA GGCGGAGCTG 
CACAACGGTA ACTCCAACTC CAGCCCACTG CTGGCCAAAC TGAGTGCGGT GGCTGCTGCC 
GGTGGCCAGT TGAGCACTCC GAGTAGCACA ACCGCTCCAT TGCCGCCGAG GCACACCTTC 
ACGGTGCCCA TATTCGCACT TCACGGACAG GGCAACTACT ACGTGCCCCT GAACGTGGAC 
TATAATGCAC TGGTGCCCTT CCTCAACGGT ATGGATCTGC TCGAGAAGAG TTACACCAGC 
ATGCCTGTAG TGCACCCCAT CAACATCAAC GTGAACTTCA TGCCCAGTTC ACCGTCCGCA 
TCGCTCTTGG CCGCTGCTGC AGCCGCCGCC GTGGCCGTTG GCAAGCAACA GCAACAACAG 
GCTGTGGTGG CCGCCGGAGC GGGACTTCCG CTGTCCACCA ACTCGGCGGC GGCTCAGGCA 
GCCGCTGTGG CCGCCGCAGC GGTGGCTAAG GCCAAACTGG AGCAGGCCAT GAACCAGAGC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247024.1
CDS	1229..3094
Translation	MNSCLADLSRLIPPQYQRKGRGRIEKTEIIEMAIRHLKHLQSECQQKESDYRSGYMDCMKEAAKFLYDVHMQDFCHRLLGRLQEHIDEMFKTDCYKSTRSCHMPDNVSASSGSPHQAYHPPLCHLRDMLATSASDVEHSQDHNDVKDLSFRNHLNQLQRSQQAAAAAAVAAAAVAVANGSSPASNAGVDSKVPLTNGGGTGGAPPAADNVPSNSTGSGSAAACAGGNSNSSGSNSSNAASSTICPPAGGSCPAKVTPLAAHQQPHQAPVITSTAPHHHHHHTDSSHHDFESSREPILHTDTSNMHSPPPRDLLLQQHPHLAHSHHTQDSLMSVRMRNYSESSHEIEHNNNYKYKNHIKERFVHELHDEETSSEHCPVAAHLQSDHSHLQALSEHSKDGTEPEIAPIMAKKRKLAEAAANGEIPLEVHTESSNAGASSANRLDKPSPSFNFSDIKDIKAELHNGNSNSSPLLAKLSAVAAAGGQLSTPSSTTAPLPPRHTFTVPIFALHGQGNYYVPLNVDYNALVPFLNGMDLLEKSYTSMPVVHPININVNFMPSSPSASLLAAAAAAAVAVGKQQQQQAVVAAGAGLPLSTNSAAAQAAAVAAAAVAKAKLEQAMNQSW

Target ORF information:

RefSeq Version	NM_001260095.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clockwork orange (cwo), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260095.2

ORF Insert Sequence:

ATGAACTCCT GCCTGGCGGA CCTGTCCCGC CTCATCCCGC CGCAGTACCA GCGCAAGGGG 
CGAGGCCGTA TCGAGAAGAC GGAGATCATC GAGATGGCCA TCAGGCACCT GAAGCACCTG 
CAGAGCGAGT GCCAGCAGAA GGAGAGCGAC TACCGGAGTG GCTACATGGA CTGTATGAAG 
GAGGCGGCCA AGTTCCTCTA CGACGTTCAC ATGCAGGACT TTTGCCACCG ACTATTGGGT 
CGCCTGCAGG AGCACATCGA TGAGATGTTC AAAACGGACT GCTACAAGTC GACGCGCAGC 
TGCCACATGC CGGATAATGT AAGCGCCTCC AGCGGCAGTC CCCATCAGGC ATACCACCCA 
CCGCTGTGCC ACCTGCGTGA CATGTTGGCC ACCTCCGCCT CGGATGTGGA GCACAGTCAG 
GACCATAACG ACGTCAAGGA TCTGAGTTTC CGGAACCATC TCAACCAGCT TCAGAGGAGT 
CAGCAGGCGG CGGCAGCCGC AGCAGTAGCG GCAGCTGCCG TTGCAGTCGC CAATGGCAGT 
TCGCCGGCTT CGAATGCCGG TGTGGATTCC AAGGTCCCGC TGACCAATGG TGGCGGCACT 
GGTGGAGCAC CGCCGGCAGC TGATAATGTG CCCAGTAATT CCACGGGCAG TGGATCAGCT 
GCCGCGTGTG CTGGCGGAAA CAGCAACAGC AGCGGCAGTA ACAGCAGCAA TGCAGCCAGT 
TCCACCATCT GTCCACCGGC CGGAGGTAGC TGTCCAGCCA AGGTCACACC ACTGGCGGCA 
CACCAGCAGC CCCACCAGGC ACCAGTGATC ACTTCGACGG CCCCACATCA TCATCACCAC 
CATACGGACA GCAGCCACCA CGACTTCGAG TCGTCCAGGG AGCCCATCCT TCACACCGAT 
ACCTCTAACA TGCACTCGCC ACCGCCCAGG GATCTGCTGC TGCAGCAGCA TCCCCACCTG 
GCCCATAGCC ACCACACCCA GGATAGTCTG ATGTCCGTGA GGATGCGCAA CTATTCGGAG 
TCCTCGCACG AGATCGAGCA CAACAACAAC TACAAGTACA AGAACCACAT CAAGGAGCGG 
TTTGTCCACG AGCTCCACGA CGAGGAGACG AGCAGCGAGC ATTGCCCGGT CGCGGCCCAC 
CTCCAGAGCG ATCACTCCCA CTTGCAGGCC TTGTCGGAGC ACTCAAAGGA CGGCACTGAA 
CCCGAGATAG CACCAATTAT GGCCAAGAAG CGGAAGTTGG CCGAGGCAGC GGCCAATGGA 
GAAATACCCC TAGAGGTTCA CACCGAGTCC AGCAACGCGG GAGCGAGTTC CGCCAACCGG 
CTGGACAAGC CCTCGCCCTC GTTCAACTTC AGCGACATCA AGGACATCAA GGCGGAGCTG 
CACAACGGTA ACTCCAACTC CAGCCCACTG CTGGCCAAAC TGAGTGCGGT GGCTGCTGCC 
GGTGGCCAGT TGAGCACTCC GAGTAGCACA ACCGCTCCAT TGCCGCCGAG GCACACCTTC 
ACGGTGCCCA TATTCGCACT TCACGGACAG GGCAACTACT ACGTGCCCCT GAACGTGGAC 
TATAATGCAC TGGTGCCCTT CCTCAACGGT ATGGATCTGC TCGAGAAGAG TTACACCAGC 
ATGCCTGTAG TGCACCCCAT CAACATCAAC GTGAACTTCA TGCCCAGTTC ACCGTCCGCA 
TCGCTCTTGG CCGCTGCTGC AGCCGCCGCC GTGGCCGTTG GCAAGCAACA GCAACAACAG 
GCTGTGGTGG CCGCCGGAGC GGGACTTCCG CTGTCCACCA ACTCGGCGGC GGCTCAGGCA 
GCCGCTGTGG CCGCCGCAGC GGTGGCTAAG GCCAAACTGG AGCAGGCCAT GAACCAGAGC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cwo ( NM_001260096.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247025.1 Drosophila melanogaster clockwork orange (cwo), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08100
Clone ID Related Accession (Same CDS sequence)	NM_001260095.2 , NM_001260096.2
Accession Version	NM_001260096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clockwork orange, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260096.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAACTCCT GCCTGGCGGA CCTGTCCCGC CTCATCCCGC CGCAGTACCA GCGCAAGGGG 
CGAGGCCGTA TCGAGAAGAC GGAGATCATC GAGATGGCCA TCAGGCACCT GAAGCACCTG 
CAGAGCGAGT GCCAGCAGAA GGAGAGCGAC TACCGGAGTG GCTACATGGA CTGTATGAAG 
GAGGCGGCCA AGTTCCTCTA CGACGTTCAC ATGCAGGACT TTTGCCACCG ACTATTGGGT 
CGCCTGCAGG AGCACATCGA TGAGATGTTC AAAACGGACT GCTACAAGTC GACGCGCAGC 
TGCCACATGC CGGATAATGT AAGCGCCTCC AGCGGCAGTC CCCATCAGGC ATACCACCCA 
CCGCTGTGCC ACCTGCGTGA CATGTTGGCC ACCTCCGCCT CGGATGTGGA GCACAGTCAG 
GACCATAACG ACGTCAAGGA TCTGAGTTTC CGGAACCATC TCAACCAGCT TCAGAGGAGT 
CAGCAGGCGG CGGCAGCCGC AGCAGTAGCG GCAGCTGCCG TTGCAGTCGC CAATGGCAGT 
TCGCCGGCTT CGAATGCCGG TGTGGATTCC AAGGTCCCGC TGACCAATGG TGGCGGCACT 
GGTGGAGCAC CGCCGGCAGC TGATAATGTG CCCAGTAATT CCACGGGCAG TGGATCAGCT 
GCCGCGTGTG CTGGCGGAAA CAGCAACAGC AGCGGCAGTA ACAGCAGCAA TGCAGCCAGT 
TCCACCATCT GTCCACCGGC CGGAGGTAGC TGTCCAGCCA AGGTCACACC ACTGGCGGCA 
CACCAGCAGC CCCACCAGGC ACCAGTGATC ACTTCGACGG CCCCACATCA TCATCACCAC 
CATACGGACA GCAGCCACCA CGACTTCGAG TCGTCCAGGG AGCCCATCCT TCACACCGAT 
ACCTCTAACA TGCACTCGCC ACCGCCCAGG GATCTGCTGC TGCAGCAGCA TCCCCACCTG 
GCCCATAGCC ACCACACCCA GGATAGTCTG ATGTCCGTGA GGATGCGCAA CTATTCGGAG 
TCCTCGCACG AGATCGAGCA CAACAACAAC TACAAGTACA AGAACCACAT CAAGGAGCGG 
TTTGTCCACG AGCTCCACGA CGAGGAGACG AGCAGCGAGC ATTGCCCGGT CGCGGCCCAC 
CTCCAGAGCG ATCACTCCCA CTTGCAGGCC TTGTCGGAGC ACTCAAAGGA CGGCACTGAA 
CCCGAGATAG CACCAATTAT GGCCAAGAAG CGGAAGTTGG CCGAGGCAGC GGCCAATGGA 
GAAATACCCC TAGAGGTTCA CACCGAGTCC AGCAACGCGG GAGCGAGTTC CGCCAACCGG 
CTGGACAAGC CCTCGCCCTC GTTCAACTTC AGCGACATCA AGGACATCAA GGCGGAGCTG 
CACAACGGTA ACTCCAACTC CAGCCCACTG CTGGCCAAAC TGAGTGCGGT GGCTGCTGCC 
GGTGGCCAGT TGAGCACTCC GAGTAGCACA ACCGCTCCAT TGCCGCCGAG GCACACCTTC 
ACGGTGCCCA TATTCGCACT TCACGGACAG GGCAACTACT ACGTGCCCCT GAACGTGGAC 
TATAATGCAC TGGTGCCCTT CCTCAACGGT ATGGATCTGC TCGAGAAGAG TTACACCAGC 
ATGCCTGTAG TGCACCCCAT CAACATCAAC GTGAACTTCA TGCCCAGTTC ACCGTCCGCA 
TCGCTCTTGG CCGCTGCTGC AGCCGCCGCC GTGGCCGTTG GCAAGCAACA GCAACAACAG 
GCTGTGGTGG CCGCCGGAGC GGGACTTCCG CTGTCCACCA ACTCGGCGGC GGCTCAGGCA 
GCCGCTGTGG CCGCCGCAGC GGTGGCTAAG GCCAAACTGG AGCAGGCCAT GAACCAGAGC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247025.1
CDS	1813..3678
Translation	MNSCLADLSRLIPPQYQRKGRGRIEKTEIIEMAIRHLKHLQSECQQKESDYRSGYMDCMKEAAKFLYDVHMQDFCHRLLGRLQEHIDEMFKTDCYKSTRSCHMPDNVSASSGSPHQAYHPPLCHLRDMLATSASDVEHSQDHNDVKDLSFRNHLNQLQRSQQAAAAAAVAAAAVAVANGSSPASNAGVDSKVPLTNGGGTGGAPPAADNVPSNSTGSGSAAACAGGNSNSSGSNSSNAASSTICPPAGGSCPAKVTPLAAHQQPHQAPVITSTAPHHHHHHTDSSHHDFESSREPILHTDTSNMHSPPPRDLLLQQHPHLAHSHHTQDSLMSVRMRNYSESSHEIEHNNNYKYKNHIKERFVHELHDEETSSEHCPVAAHLQSDHSHLQALSEHSKDGTEPEIAPIMAKKRKLAEAAANGEIPLEVHTESSNAGASSANRLDKPSPSFNFSDIKDIKAELHNGNSNSSPLLAKLSAVAAAGGQLSTPSSTTAPLPPRHTFTVPIFALHGQGNYYVPLNVDYNALVPFLNGMDLLEKSYTSMPVVHPININVNFMPSSPSASLLAAAAAAAVAVGKQQQQQAVVAAGAGLPLSTNSAAAQAAAVAAAAVAKAKLEQAMNQSW

Target ORF information:

RefSeq Version	NM_001260096.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clockwork orange (cwo), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260096.2

ORF Insert Sequence:

ATGAACTCCT GCCTGGCGGA CCTGTCCCGC CTCATCCCGC CGCAGTACCA GCGCAAGGGG 
CGAGGCCGTA TCGAGAAGAC GGAGATCATC GAGATGGCCA TCAGGCACCT GAAGCACCTG 
CAGAGCGAGT GCCAGCAGAA GGAGAGCGAC TACCGGAGTG GCTACATGGA CTGTATGAAG 
GAGGCGGCCA AGTTCCTCTA CGACGTTCAC ATGCAGGACT TTTGCCACCG ACTATTGGGT 
CGCCTGCAGG AGCACATCGA TGAGATGTTC AAAACGGACT GCTACAAGTC GACGCGCAGC 
TGCCACATGC CGGATAATGT AAGCGCCTCC AGCGGCAGTC CCCATCAGGC ATACCACCCA 
CCGCTGTGCC ACCTGCGTGA CATGTTGGCC ACCTCCGCCT CGGATGTGGA GCACAGTCAG 
GACCATAACG ACGTCAAGGA TCTGAGTTTC CGGAACCATC TCAACCAGCT TCAGAGGAGT 
CAGCAGGCGG CGGCAGCCGC AGCAGTAGCG GCAGCTGCCG TTGCAGTCGC CAATGGCAGT 
TCGCCGGCTT CGAATGCCGG TGTGGATTCC AAGGTCCCGC TGACCAATGG TGGCGGCACT 
GGTGGAGCAC CGCCGGCAGC TGATAATGTG CCCAGTAATT CCACGGGCAG TGGATCAGCT 
GCCGCGTGTG CTGGCGGAAA CAGCAACAGC AGCGGCAGTA ACAGCAGCAA TGCAGCCAGT 
TCCACCATCT GTCCACCGGC CGGAGGTAGC TGTCCAGCCA AGGTCACACC ACTGGCGGCA 
CACCAGCAGC CCCACCAGGC ACCAGTGATC ACTTCGACGG CCCCACATCA TCATCACCAC 
CATACGGACA GCAGCCACCA CGACTTCGAG TCGTCCAGGG AGCCCATCCT TCACACCGAT 
ACCTCTAACA TGCACTCGCC ACCGCCCAGG GATCTGCTGC TGCAGCAGCA TCCCCACCTG 
GCCCATAGCC ACCACACCCA GGATAGTCTG ATGTCCGTGA GGATGCGCAA CTATTCGGAG 
TCCTCGCACG AGATCGAGCA CAACAACAAC TACAAGTACA AGAACCACAT CAAGGAGCGG 
TTTGTCCACG AGCTCCACGA CGAGGAGACG AGCAGCGAGC ATTGCCCGGT CGCGGCCCAC 
CTCCAGAGCG ATCACTCCCA CTTGCAGGCC TTGTCGGAGC ACTCAAAGGA CGGCACTGAA 
CCCGAGATAG CACCAATTAT GGCCAAGAAG CGGAAGTTGG CCGAGGCAGC GGCCAATGGA 
GAAATACCCC TAGAGGTTCA CACCGAGTCC AGCAACGCGG GAGCGAGTTC CGCCAACCGG 
CTGGACAAGC CCTCGCCCTC GTTCAACTTC AGCGACATCA AGGACATCAA GGCGGAGCTG 
CACAACGGTA ACTCCAACTC CAGCCCACTG CTGGCCAAAC TGAGTGCGGT GGCTGCTGCC 
GGTGGCCAGT TGAGCACTCC GAGTAGCACA ACCGCTCCAT TGCCGCCGAG GCACACCTTC 
ACGGTGCCCA TATTCGCACT TCACGGACAG GGCAACTACT ACGTGCCCCT GAACGTGGAC 
TATAATGCAC TGGTGCCCTT CCTCAACGGT ATGGATCTGC TCGAGAAGAG TTACACCAGC 
ATGCCTGTAG TGCACCCCAT CAACATCAAC GTGAACTTCA TGCCCAGTTC ACCGTCCGCA 
TCGCTCTTGG CCGCTGCTGC AGCCGCCGCC GTGGCCGTTG GCAAGCAACA GCAACAACAG 
GCTGTGGTGG CCGCCGGAGC GGGACTTCCG CTGTCCACCA ACTCGGCGGC GGCTCAGGCA 
GCCGCTGTGG CCGCCGCAGC GGTGGCTAAG GCCAAACTGG AGCAGGCCAT GAACCAGAGC 
TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cwo ( NM_080036.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524775.1 Drosophila melanogaster clockwork orange (cwo), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06859
Clone ID Related Accession (Same CDS sequence)	NM_080036.3
Accession Version	NM_080036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	clockwork orange, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_080036.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGCCCT ATTGGAACGA GACGAACGGA CATGCCGCCC ATCCGGTCAA GTATGAGAGC 
GAAGCAGCTG TCTCCAGTTT TCCTTATTGC ACAGAATCTA GTTTAAATTT TTCAACATCA 
GCCACGGCAT ACAGCGAGGA CGATGCTGAA TATGCCACCG GAAGACGTAA TAAGACATCG 
AGGCAAGATC CACTTTCCCA TCGAATCATT GAGAAACGGA GACGAGATCG CATGAACTCC 
TGCCTGGCGG ACCTGTCCCG CCTCATCCCG CCGCAGTACC AGCGCAAGGG GCGAGGCCGT 
ATCGAGAAGA CGGAGATCAT CGAGATGGCC ATCAGGCACC TGAAGCACCT GCAGAGCGAG 
TGCCAGCAGA AGGAGAGCGA CTACCGGAGT GGCTACATGG ACTGTATGAA GGAGGCGGCC 
AAGTTCCTCT ACGACGTTCA CATGCAGGAC TTTTGCCACC GACTATTGGG TCGCCTGCAG 
GAGCACATCG ATGAGATGTT CAAAACGGAC TGCTACAAGT CGACGCGCAG CTGCCACATG 
CCGGATAATG TAAGCGCCTC CAGCGGCAGT CCCCATCAGG CATACCACCC ACCGCTGTGC 
CACCTGCGTG ACATGTTGGC CACCTCCGCC TCGGATGTGG AGCACAGTCA GGACCATAAC 
GACGTCAAGG ATCTGAGTTT CCGGAACCAT CTCAACCAGC TTCAGAGGAG TCAGCAGGCG 
GCGGCAGCCG CAGCAGTAGC GGCAGCTGCC GTTGCAGTCG CCAATGGCAG TTCGCCGGCT 
TCGAATGCCG GTGTGGATTC CAAGGTCCCG CTGACCAATG GTGGCGGCAC TGGTGGAGCA 
CCGCCGGCAG CTGATAATGT GCCCAGTAAT TCCACGGGCA GTGGATCAGC TGCCGCGTGT 
GCTGGCGGAA ACAGCAACAG CAGCGGCAGT AACAGCAGCA ATGCAGCCAG TTCCACCATC 
TGTCCACCGG CCGGAGGTAG CTGTCCAGCC AAGGTCACAC CACTGGCGGC ACACCAGCAG 
CCCCACCAGG CACCAGTGAT CACTTCGACG GCCCCACATC ATCATCACCA CCATACGGAC 
AGCAGCCACC ACGACTTCGA GTCGTCCAGG GAGCCCATCC TTCACACCGA TACCTCTAAC 
ATGCACTCGC CACCGCCCAG GGATCTGCTG CTGCAGCAGC ATCCCCACCT GGCCCATAGC 
CACCACACCC AGGATAGTCT GATGTCCGTG AGGATGCGCA ACTATTCGGA GTCCTCGCAC 
GAGATCGAGC ACAACAACAA CTACAAGTAC AAGAACCACA TCAAGGAGCG GTTTGTCCAC 
GAGCTCCACG ACGAGGAGAC GAGCAGCGAG CATTGCCCGG TCGCGGCCCA CCTCCAGAGC 
GATCACTCCC ACTTGCAGGC CTTGTCGGAG CACTCAAAGG ACGGCACTGA ACCCGAGATA 
GCACCAATTA TGGCCAAGAA GCGGAAGTTG GCCGAGGCAG CGGCCAATGG AGAAATACCC 
CTAGAGGTTC ACACCGAGTC CAGCAACGCG GGAGCGAGTT CCGCCAACCG GCTGGACAAG 
CCCTCGCCCT CGTTCAACTT CAGCGACATC AAGGACATCA AGGCGGAGCT GCACAACGGT 
AACTCCAACT CCAGCCCACT GCTGGCCAAA CTGAGTGCGG TGGCTGCTGC CGGTGGCCAG 
TTGAGCACTC CGAGTAGCAC AACCGCTCCA TTGCCGCCGA GGCACACCTT CACGGTGCCC 
ATATTCGCAC TTCACGGACA GGGCAACTAC TACGTGCCCC TGAACGTGGA CTATAATGCA 
CTGGTGCCCT TCCTCAACGG TATGGATCTG CTCGAGAAGA GTTACACCAG CATGCCTGTA 
GTGCACCCCA TCAACATCAA CGTGAACTTC ATGCCCAGTT CACCGTCCGC ATCGCTCTTG 
GCCGCTGCTG CAGCCGCCGC CGTGGCCGTT GGCAAGCAAC AGCAACAACA GGCTGTGGTG 
GCCGCCGGAG CGGGACTTCC GCTGTCCACC AACTCGGCGG CGGCTCAGGC AGCCGCTGTG 
GCCGCCGCAG CGGTGGCTAA GGCCAAACTG GAGCAGGCCA TGAACCAGAG CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524775.1
CDS	1122..3218
Translation	MEPYWNETNGHAAHPVKYESEAAVSSFPYCTESSLNFSTSATAYSEDDAEYATGRRNKTSRQDPLSHRIIEKRRRDRMNSCLADLSRLIPPQYQRKGRGRIEKTEIIEMAIRHLKHLQSECQQKESDYRSGYMDCMKEAAKFLYDVHMQDFCHRLLGRLQEHIDEMFKTDCYKSTRSCHMPDNVSASSGSPHQAYHPPLCHLRDMLATSASDVEHSQDHNDVKDLSFRNHLNQLQRSQQAAAAAAVAAAAVAVANGSSPASNAGVDSKVPLTNGGGTGGAPPAADNVPSNSTGSGSAAACAGGNSNSSGSNSSNAASSTICPPAGGSCPAKVTPLAAHQQPHQAPVITSTAPHHHHHHTDSSHHDFESSREPILHTDTSNMHSPPPRDLLLQQHPHLAHSHHTQDSLMSVRMRNYSESSHEIEHNNNYKYKNHIKERFVHELHDEETSSEHCPVAAHLQSDHSHLQALSEHSKDGTEPEIAPIMAKKRKLAEAAANGEIPLEVHTESSNAGASSANRLDKPSPSFNFSDIKDIKAELHNGNSNSSPLLAKLSAVAAAGGQLSTPSSTTAPLPPRHTFTVPIFALHGQGNYYVPLNVDYNALVPFLNGMDLLEKSYTSMPVVHPININVNFMPSSPSASLLAAAAAAAVAVGKQQQQQAVVAAGAGLPLSTNSAAAQAAAVAAAAVAKAKLEQAMNQSW

Target ORF information:

RefSeq Version	NM_080036.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster clockwork orange (cwo), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080036.3

ORF Insert Sequence:

ATGGAGCCCT ATTGGAACGA GACGAACGGA CATGCCGCCC ATCCGGTCAA GTATGAGAGC 
GAAGCAGCTG TCTCCAGTTT TCCTTATTGC ACAGAATCTA GTTTAAATTT TTCAACATCA 
GCCACGGCAT ACAGCGAGGA CGATGCTGAA TATGCCACCG GAAGACGTAA TAAGACATCG 
AGGCAAGATC CACTTTCCCA TCGAATCATT GAGAAACGGA GACGAGATCG CATGAACTCC 
TGCCTGGCGG ACCTGTCCCG CCTCATCCCG CCGCAGTACC AGCGCAAGGG GCGAGGCCGT 
ATCGAGAAGA CGGAGATCAT CGAGATGGCC ATCAGGCACC TGAAGCACCT GCAGAGCGAG 
TGCCAGCAGA AGGAGAGCGA CTACCGGAGT GGCTACATGG ACTGTATGAA GGAGGCGGCC 
AAGTTCCTCT ACGACGTTCA CATGCAGGAC TTTTGCCACC GACTATTGGG TCGCCTGCAG 
GAGCACATCG ATGAGATGTT CAAAACGGAC TGCTACAAGT CGACGCGCAG CTGCCACATG 
CCGGATAATG TAAGCGCCTC CAGCGGCAGT CCCCATCAGG CATACCACCC ACCGCTGTGC 
CACCTGCGTG ACATGTTGGC CACCTCCGCC TCGGATGTGG AGCACAGTCA GGACCATAAC 
GACGTCAAGG ATCTGAGTTT CCGGAACCAT CTCAACCAGC TTCAGAGGAG TCAGCAGGCG 
GCGGCAGCCG CAGCAGTAGC GGCAGCTGCC GTTGCAGTCG CCAATGGCAG TTCGCCGGCT 
TCGAATGCCG GTGTGGATTC CAAGGTCCCG CTGACCAATG GTGGCGGCAC TGGTGGAGCA 
CCGCCGGCAG CTGATAATGT GCCCAGTAAT TCCACGGGCA GTGGATCAGC TGCCGCGTGT 
GCTGGCGGAA ACAGCAACAG CAGCGGCAGT AACAGCAGCA ATGCAGCCAG TTCCACCATC 
TGTCCACCGG CCGGAGGTAG CTGTCCAGCC AAGGTCACAC CACTGGCGGC ACACCAGCAG 
CCCCACCAGG CACCAGTGAT CACTTCGACG GCCCCACATC ATCATCACCA CCATACGGAC 
AGCAGCCACC ACGACTTCGA GTCGTCCAGG GAGCCCATCC TTCACACCGA TACCTCTAAC 
ATGCACTCGC CACCGCCCAG GGATCTGCTG CTGCAGCAGC ATCCCCACCT GGCCCATAGC 
CACCACACCC AGGATAGTCT GATGTCCGTG AGGATGCGCA ACTATTCGGA GTCCTCGCAC 
GAGATCGAGC ACAACAACAA CTACAAGTAC AAGAACCACA TCAAGGAGCG GTTTGTCCAC 
GAGCTCCACG ACGAGGAGAC GAGCAGCGAG CATTGCCCGG TCGCGGCCCA CCTCCAGAGC 
GATCACTCCC ACTTGCAGGC CTTGTCGGAG CACTCAAAGG ACGGCACTGA ACCCGAGATA 
GCACCAATTA TGGCCAAGAA GCGGAAGTTG GCCGAGGCAG CGGCCAATGG AGAAATACCC 
CTAGAGGTTC ACACCGAGTC CAGCAACGCG GGAGCGAGTT CCGCCAACCG GCTGGACAAG 
CCCTCGCCCT CGTTCAACTT CAGCGACATC AAGGACATCA AGGCGGAGCT GCACAACGGT 
AACTCCAACT CCAGCCCACT GCTGGCCAAA CTGAGTGCGG TGGCTGCTGC CGGTGGCCAG 
TTGAGCACTC CGAGTAGCAC AACCGCTCCA TTGCCGCCGA GGCACACCTT CACGGTGCCC 
ATATTCGCAC TTCACGGACA GGGCAACTAC TACGTGCCCC TGAACGTGGA CTATAATGCA 
CTGGTGCCCT TCCTCAACGG TATGGATCTG CTCGAGAAGA GTTACACCAG CATGCCTGTA 
GTGCACCCCA TCAACATCAA CGTGAACTTC ATGCCCAGTT CACCGTCCGC ATCGCTCTTG 
GCCGCTGCTG CAGCCGCCGC CGTGGCCGTT GGCAAGCAAC AGCAACAACA GGCTGTGGTG 
GCCGCCGGAG CGGGACTTCC GCTGTCCACC AACTCGGCGG CGGCTCAGGC AGCCGCTGTG 
GCCGCCGCAG CGGTGGCTAA GGCCAAACTG GAGCAGGCCA TGAACCAGAG CTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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cwo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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