ct cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ct
Entrez Gene ID	44540
Full Name	cut
Synonyms	BcDNA:GH10590,CG11387,CT,CUT,Ct,Cut,Dmel\CG11387,kf,l(1)7Ba,l(1)7Bb,l(1)VE614
General protein information	Preferred Names cut Names CG11387 gene product from transcript CG11387-RE CG11387-PA CG11387-PC CG11387-PD CG11387-PE Cut ct-PA ct-PC ct-PD ct-PE kinked-femur
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7B4-7B6\|1-20.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272381.1	NP_001259310.1	cut, isoform E
NM_001258636.2	NP_001245565.1	cut, isoform D
NM_001272381.2	NP_001259310.1	cut, isoform E
NM_001144702.2	NP_001138174.1	cut, isoform C
NM_080025.2	NP_524764.1	cut, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

and ecdysone signaling pathways synergistically regulate Cut expression for proper DV boundary formation in the wing disc
Title: The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal-Ventral Boundary in Drosophila Wing Discs.

Loss of Ct and Hth in the antenna disc resulted in ectopic eye development in the antenna.
Title: Segregation of eye and antenna fates maintained by mutual antagonism in Drosophila.

Drosophila transcription factor Cut coordinately controls differentiation and repression of apoptosis via direct regulation of the pro-apoptotic gene
Title: Antagonistic regulation of apoptosis and differentiation by the Cut transcription factor represents a tumor-suppressing mechanism in Drosophila.

Turtle is downstream effector of Cut-mediated regulation of da neuron dendrite morphology.
Title: Turtle functions downstream of Cut in differentially regulating class specific dendrite morphogenesis in Drosophila.

Study of distant interactions of LCR from the locus CUT.
Title: Study of distant interactions of LCR from the Drosophila melanogaster locus CUT.

The following ct gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ct cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02347	NM_001144702.2 Latest version!	Drosophila melanogaster cut (ct), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
OFa02348	NM_080025.2 Latest version!	Drosophila melanogaster cut (ct), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03735	NM_001272381.2 Latest version!	Drosophila melanogaster cut (ct), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa03736	NM_001258636.2 Latest version!	Drosophila melanogaster cut (ct), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
OFa03735	NM_001272381.1	Drosophila melanogaster cut (ct), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ct ( NM_001144702.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138174.1 Drosophila melanogaster cut (ct), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa02347
Clone ID Related Accession (Same CDS sequence)	NM_001144702.2
Accession Version	NM_001144702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-15
Organism	Drosophila melanogaster(fruit fly)
Product	cut, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_001144702.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGATGCCAA TACACGACAT CCATCCAATC ACCCAACAAC CAACCCCCCT CGACAAAGTA 
ATACTCCCAC TTCATTGCTC TCATTCAGAA TTCCGCGCAA CCCTTGCCGA AAAGGAAGTG 
AACACACTCA AAGAGCAGCT ATCCACCGGC AATCCCGACA GCAACCTCAA CAGCGAAAAC 
AGCGATACAG CAGCAGCGGC AGCAACAGCA GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA 
ACAGCAACAA ACGACATCGA GGACGAGCAG CAGCAACAGT TGCAGCAGAC GGCCAGCGGC 
GGCATTTTGG AAAGCGACAG CGACAAGCTG CTGAACAGCT CCATTGTTGC AGCGGCCATA 
ACGCTGCAGC AGCAGAACGG CAGCAATCTG CTGGCCAACA CAAATACGCC GTCGCCCTCG 
CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC 
GGTGTTGGCG GCGCCTGCCT CAATCCCAAG CTTTTCTTCA ATCACGCCCA ACAGATGATG 
ATGATGGAGG CGGCAGCCGC GGCAGCAGCG GCGGCATTGC AACAGCAGCA GCAACAGCAA 
TCACCACTCC ATTCGCCGGC GAATGAAGTT GCGATTCCCA CAGAACAGCC AGCGGCAACA 
GTGGCAACAG GAGCCGCTGC TGCAGCAGCC GCAGCAGCAA CACCGATTGC AACTGGCAAC 
GTCAAAAGCG GCAGCACCAC CAGCAACGCC AATCACACCA ACAGCAACAA TAGTCACCAG 
GACGAGGAGG AGCTGGACGA TGAGGAGGAG GACGAGGAGG AGGATGAGGA CGAGGATGAT 
GAGGAGGAGA ATGCCTCGAT GCAATCGAAT GCTGATGATA TGGAGCTGGA TGCGCAGCAA 
GAAACCAGAA CTGAGCCAAG TGCAACAACA CAACAGCAGC ATCAGCAGCA GGATACAGAG 
GATCTTGAGG AGAACAAGGA TGCGGGGGAG GCTAGTTTAA ATGTTAGCAA TAATCACAAT 
ACAACCGATA GCAATAATAG TTGTAGTCGA AAGAACAACA ATGGCGGCAA TGAAAGTGAG 
CAACATGTGG CCAGTTCGGC GGAGGACGAT GATTGCGCCA ACAACAATAC AAATACCAGC 
AATAACAACA ATACCAGCAA CACCGCCACC AGCAACACGA ACAACAACAA TAATAATAAC 
AGCAGCAGCG GCAACAGCGA GAAGCGCAAG AAGAAGAACA ATAACAACAA CAACGGCCAG 
CCTGCTGTTC TATTAGCTGC CAAAGATAAA GAGATTAAAG CCCTTTTGGA CGAACTGCAG 
CGCTTGAGGG CGCAGGAGCA GACGCACCTG GTGCAAATCC AACGATTGGA GGAACATCTT 
GAGGTCAAGC GTCAGCACAT CATCCGATTG GAGGCACGTC TGGATAAGCA GCAAATTAAT 
GAGGCACTGG CCGAGGCGAC CGCTTTGTCC GCTGCCGCGT CAACCAACAA CAACAATAAT 
AGCCAGAGCA GCGACAACAA TAAGAAGCTG AATACCGCTG CAGAAAGACC GATGGATGCG 
TCATCCAATG CCGATCTACC GGAGTCCACA AAAGCTCCAG TTCCGGCGGA GGATGACGAA 
GAAGATGAGG ATCAGGCCAT GCTGGTGGAT TCCGAGGAAG CGGAGGACAA GCCAGAGGAC 
TCACATCACG ATGACGACGA AGATGAGGAC GAGGATCGGG AGGCGGTCAA TGCCACCACA 
ACCGATAGCA ACGAGCTCAA AATCAAAAAG GAACAGCACA GTCCGCTGGA TCTGAATGTG 
CTCAGTCCGA ATTCGGCCAT TGCCGCTGCA GCTGCGGCCG CCGCTGCCGC CGCCTGCGCC 
AATGACCCCA ACAAATTCCA GGCTCTGCTC ATCGAGCGGA CCAAAGCCTT GGCCGCAGAG 
GCGCTCAAGA ATGGCGCCAG CGATGCCCTC AGCGAAGAAA CCAACCGAAA TCATGAAGAC 
GAAGACGAAG TGTCGCCAGC AGCAACTGCA ACTGCAACAC TCGCAACGCC TGCAACAGTG 
GCAACACCAA TTGAAACACT GGCCACCGAT TCGGAAGCAA AAGCCAGCAA TGACATGGCG 
CCCAAGCAGG AAATCGAAGA AGTGGATGAC GAGGATGCCA CCGATGAACC GGTGATCCTG 
CCGGAGCAAC TAGTCAACGA CTATTGGCGA CGGGGCTTTG TCGAATCGGG GAATAGCACT 
AATACGAGCA CCGCTGCAAT GCCAGGGAGC AAAACGCCAA CAATTTATCA AAATTTTAAG 
GTCGAGCAGC AGGCACAGTT GCTGCCGCCA CCGCCGCCGC CACCACCACC CCCGCAGCAA 
CAACATCAAC AGCAGCAACA ACAACAACAT CTACACTTTT CACAGCAACA GCAACAACAA 
CATCAGCAGC AACATGGACC ATCGTCCACA GCCCTGCTTA GTCAACTGGT TAACTCAACA 
TTTTCCAACT CTTCCAACTC TTCTCGCCTA GATGCCCACC ATCAGCAGCA GCAGCACCAC 
CAACAGCAGC ACCAACACCA GCAACAACAC CACCAGCAAC AGCACCTCCA CCAGCAACAT 
CACCATCACC TGCAACAGCA ACCAAACAGC GGCAGCAACA GCAATCCAGC GAGCAACGAC 
CACCATCATG GACACCATTT GCATGGCCAT GGTTTGCTGC ATCCTTCGTC GGCGCACCAT 
CTGCACCACC AAACGACCGA ATCCAATTCG AATTCGAGTA CACCCACAGC GGCGGGAAAT 
AACAATGGTA GCAATAACAG CTCCAGCAAC ACAAATGCCA ATAGTACCGC TCAATTGGCC 
GCCAGTTTGG CCAGTACACT CAATGGCACC AAGTCCCTGA TGCAGGAGGA TAGCAATGGC 
TTGGCCGCCG TCGCCATGGC GGCTCATGCC CAACATGCGG CTGCCTTGGG ACCTGGATTT 
TTGCCCGGCC TTCCAGCGTT TCAGTTTGCC GCCGCCCAAG TGGCCGCCGG TGGCGATGGA 
CGGGGTCATT ATCGTTTCGC CGACTCGGAA CTGCAATTGC CACCGGGTGC TTCGATGGCC 
GGTCGTCTTG GAGAGTCGCT CATACCCAAG GGTGATCCCA TGGAGGCCAA ACTCCAAGAG 
ATGTTGCGCT ACAACATGGA CAAGTACGCC AATCAGGCGC TGGATACCCT GCACATTTCG 
CGACGTGTCC GCGAACTGCT ATCGGTGCAC AACATTGGCC AGCGACTATT TGCCAAATAC 
ATACTGGGCC TGTCGCAGGG CACCGTTTCC GAGCTGCTGA GCAAACCCAA GCCGTGGGAC 
AAGTTGACGG AGAAGGGACG AGATAGCTAC CGCAAAATGC ACGCCTGGGC CTGTGACGAT 
AATGCCGTCA TGCTGCTCAA ATCGCTGATA CCCAAGAAAG ATTCTGGACT GCCGCAGTAT 
GCGGGTCGTG GTGCCGGCGG CGCTGGCGGC GATGATTCCA TGTCCGAGGA TCGGATTGCA 
CATATCCTCA GCGAAGCCTC CTCGCTGATG AAACAGAGCA GTGTGGCCCA GCATCGGGAG 
CAGGAGCGTC GCAGTCACGG CGGCGAGGAT TCACACAGCA ACGAGGACAG CAAATCGCCA 
CCTCAAAGCT GCACGTCGCC GTTCTTCAAG GTGGAGAATC AGCTGAAGCA GCACCAGCAT 
CTTAATCCCG AGCAGGCTGC CGCTCAGCAA AGGGAAAGGG AACGCGAGCA AAGAGAACGG 
GAGCAGCAGC AGCGGCTGCG GCACGACGAC CAGGACAAGA TGGCTCGTCT CTACCAGGAG 
CTGATTGCAC GCACTCCGCG TGAGACGGCT TTTCCAAGCT TTCTCTTCTC ACCCTCGCTA 
TTTGGAGGAG CCGCCGGAAT GCCAGGAGCA GCCAGCAATG CCTTTCCCGC CATGGCCGAT 
GAAAATATGC GTCACGTCTT TGAACGTGAG ATTGCCAAGC TGCAGCAGCA CCAACAGCAA 
CAACAAGCTG CCCAGGCTCA GGCACAGTTC CCCAACTTCT CGAGCCTGAT GGCTTTGCAG 
CAGCAGGTCC TCAACGGAGC CCAGGATCTC TCGCTAGCTG CCGCTGCAGC CAAGGACATC 
AAGTTGAACG GTCAGCGATC CTCGTTGGAG CACAGTGCCG GCAGCAGCAG TTGTTCAAAA 
GATGGCGAAC GAGATGATGC GTATCCAAGT TCCCTACATG GTCGCAAGTC CGAGGGAGGT 
GGCACACCCG CACCACCTGC CCCACCATCT GGCCCAGGAA CCGGAGCAGG AGCCCCACCC 
ACAGCAGCAC CACCAACCGG CGGTGCCAGC AGCAATTCGG CAGCACCCAG TCCGCTAAGC 
AATTCAATAC TTCCACCGGC ATTGAGCAGC CAAGGTGAGG AGTTCGCCGC GACAGCAAGT 
CCTTTGCAGC GAATGGCCTC GATCACCAAT TCGCTGATTA CCCAACCACC GGTGACGCCG 
CACCACAGTA CGCCACAGCG ACCCACCAAG GCGGTTCTGC CACCGATCAC CCAGCAACAG 
TTCGATATGT TCAACAACCT TAACACCGAG GATATAGTCA GACGTGTGAA GGAGGCCCTG 
TCGCAGTACT CCATCTCCCA GCGATTGTTC GGCGAGTCCG TGCTGGGTTT GTCTCAGGGA 
TCGGTCTCCG ATTTATTGGC TAGACCCAAG CCATGGCACA TGCTCACTCA AAAGGGCCGT 
GAACCCTTCA TTCGCATGAA GATGTTCCTG GAGGATGAGA ACGCGGTGCA CAAACTGGTG 
GCCAGTCAAT ACAAGATTGC CCCCGAGAAG CTAATGCGAA CGGGCAGTTA CAGTGGAAGT 
CCGCAGATGC CACAGGGATT GGCAAGCAAA ATGCAGGCTG CCTCCCTGCC CATGCAGAAG 
ATGATGAGCG AACTTAAGCT GCAGGAGCCA GCACAGGCGC AGCACTTGAT GCAGCAAATG 
CAGGCGGCGG CAATGTCGGC AGCAATGCAA CAGCAGCAAG TGGCACAAGC GCAGCAGCAG 
GCTCAGCAGG CGCAACAGGC ACAACAGCAT TTGCAGCAGC AGGCGCAGCA GCACTTGCAA 
CAGCAGCAAC ATCTTGCCCA GCAGCAACAC CCCCATCAGC AGCACCATCA AGCGGCCGCC 
GCGGCTGCCG CATTGCACCA CCAAAGCATG CTTCTGACCT CGCCCGGACT GCCACCCCAG 
CATGCCATCA GTCTGCCACC TTCAGCGGGT GGAGCCCAAC CAGGTGGTCC TGGTGGCAAT 
CAGGGCAGCT CAAATCCCTC AAACAGCGAA AAGAAACCCA TGCTGATGCC GGTTCATGGC 
ACTAATGCCA TGAGAAGTCT GCACCAGCAC ATGTCGCCCA CCGTTTACGA AATGGCCGCT 
TTAACCCAAG ATCTGGACAC CCACGATATC ACTACCAAGA TCAAGGAGGC GTTGTTGGCC 
AACAATATTG GTCAGAAAAT ATTTGGCGAA GCCGTTTTGG GACTTTCGCA GGGATCCGTA 
TCTGAGCTGC TGAGCAAACC GAAGCCCTGG CACATGCTCT CCATCAAGGG CAGGGAACCC 
TTCATCCGGA TGCAATTGTG GCTCAGTGAT GCCAACAATG TTGAACGATT GCAGTTGCTG 
AAAAATGAGC GACGGGAGGC CAGCAAACGA AGGAGGAGCA CAGGCCCAAA CCAACAGGAC 
AATAGTAGCG ATACCTCCAG CAATGATACC AATGACTTCT ATACCAGCAG TCCGGGTCCA 
GGATCCGTCG GTTCGGGTGT CGGTGGTGCT CCGCCGAGCA AAAAACAGCG TGTCCTCTTC 
TCCGAGGAGC AAAAGGAGGC ACTTCGGCTG GCATTCGCCT TGGATCCGTA TCCAAATGTG 
GGGACCATCG AGTTTCTGGC CAATGAGCTG GGCTTGGCAA CGCGGACGAT CACCAACTGG 
TTCCACAATC ATCGCATGCG ATTGAAGCAA CAGGTGCCAC ACGGACCTGC CGGTCAAGAT 
AATCCGATAC CGAGTCGCGA GAGTACCAGT GCCACGCCCT TCGATCCCGT CCAATTCCGC 
ATTCTGCTGC AGCAGCGTCT ACTGGAGCTC CACAAGGAGC GAATGGGCAT GAGCGGTGCC 
CCCATTCCCT ATCCGCCGTA CTTTGCTGCC GCTGCCATTT TGGGTCGCAG TCTGGCGGGG 
ATTCCCGGTG CGGCAGCGGC CGCTGGAGCA GCTGCAGCAG CCGCCGCAGT TGGCGCCTCT 
GGTGGTGATG AGTTGCAGGC TCTGAATCAG GCCTTCAAGG AGCAGATGAG CGGTTTGGAT 
CTGTCGATGC CCACCTTAAA GCGGGAACGC AGCGATGACT ATCAGGACGA TCTGGAACTG 
GAGGGTGGTG GTCACAATCT AAGTGACAAC GAATCGCTGG AGGGTCAAGA GCCGGAGGAC 
AAGACCACCG ATTACGAGAA GGTGCTGCAC AAATCCGCTT TGGCCGCTGC CGCCGCTTAC 
ATGTCCAATG CAGTGCGGAG TTCACGACGA AAGCCAGCTG CCCCACAGTG GGTCAATCCC 
GCCGGAGCGG TGACCAATCC AAGTGCTGTA GTTGCAGCCG TTGCTGCTGC CGCAGCTGCC 
GCGGCGGACA ATGAACGCAT CATCAACGGG GTGTGCGTGA TGCAGGCCTC CGAGTACGGC 
CGCGATGATA CTGATAGCAA CAAGCCAACG GACGGCGGCA ACGATTCCGA TCATGAGCAC 
GCCCAGCTGG AGATCGATCA GCGTTTCATG GAACCGGAGG TGCACATCAA ACAGGAGGAG 
GACGACGATG AGGAGCAATC GGGATCGGTT AATCTGGACA ACGAGGACAA TGCGACTAGC 
GAGCAAAAAC TGAAGGTGAT CAACGAGGAG AAGCTGCGAA TGGTGCGAGT TCGCCGGCTG 
AGCAGCACTG GTGGTGGTTC CTCTGAGGAA ATGCCAGCTC CCCTCGCACC ACCGCCTCCT 
CCGCCAGCAG CTTCTTCTTC TATCGTTAGT GGAGAAAGCA CCACCAGCAG CAGTAGTAGC 
AGCAACACCA GCAGCAGCAC ACCGGCGGTA ACCACTGCAG CAGCAACTGC GGCAGCCGGT 
TGGAACTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138174.1
CDS	269..7420
Translation	MQPTLPQAAGTADMDLTAVQSINDWFFKKEQIYLLAQFWQQMPIHDIHPITQQPTPLDKVILPLHCSHSEFRATLAEKEVNTLKEQLSTGNPDSNLNSENSDTAAAAATAAAVAAVVAGATATNDIEDEQQQQLQQTASGGILESDSDKLLNSSIVAAAITLQQQNGSNLLANTNTPSPSPPLLSAEQQQQLQSSLQQSGGVGGACLNPKLFFNHAQQMMMMEAAAAAAAAALQQQQQQQSPLHSPANEVAIPTEQPAATVATGAAAAAAAAATPIATGNVKSGSTTSNANHTNSNNSHQDEEELDDEEEDEEEDEDEDDEEENASMQSNADDMELDAQQETRTEPSATTQQQHQQQDTEDLEENKDAGEASLNVSNNHNTTDSNNSCSRKNNNGGNESEQHVASSAEDDDCANNNTNTSNNNNTSNTATSNTNNNNNNNSSSGNSEKRKKKNNNNNNGQPAVLLAAKDKEIKALLDELQRLRAQEQTHLVQIQRLEEHLEVKRQHIIRLEARLDKQQINEALAEATALSAAASTNNNNNSQSSDNNKKLNTAAERPMDASSNADLPESTKAPVPAEDDEEDEDQAMLVDSEEAEDKPEDSHHDDDEDEDEDREAVNATTTDSNELKIKKEQHSPLDLNVLSPNSAIAAAAAAAAAAACANDPNKFQALLIERTKALAAEALKNGASDALSEETNRNHEDEDEVSPAATATATLATPATVATPIETLATDSEAKASNDMAPKQEIEEVDDEDATDEPVILPEQLVNDYWRRGFVESGNSTNTSTAAMPGSKTPTIYQNFKVEQQAQLLPPPPPPPPPPQQQHQQQQQQQHLHFSQQQQQQHQQQHGPSSTALLSQLVNSTFSNSSNSSRLDAHHQQQQHHQQQHQHQQQHHQQQHLHQQHHHHLQQQPNSGSNSNPASNDHHHGHHLHGHGLLHPSSAHHLHHQTTESNSNSSTPTAAGNNNGSNNSSSNTNANSTAQLAASLASTLNGTKSLMQEDSNGLAAVAMAAHAQHAAALGPGFLPGLPAFQFAAAQVAAGGDGRGHYRFADSELQLPPGASMAGRLGESLIPKGDPMEAKLQEMLRYNMDKYANQALDTLHISRRVRELLSVHNIGQRLFAKYILGLSQGTVSELLSKPKPWDKLTEKGRDSYRKMHAWACDDNAVMLLKSLIPKKDSGLPQYAGRGAGGAGGDDSMSEDRIAHILSEASSLMKQSSVAQHREQERRSHGGEDSHSNEDSKSPPQSCTSPFFKVENQLKQHQHLNPEQAAAQQREREREQREREQQQRLRHDDQDKMARLYQELIARTPRETAFPSFLFSPSLFGGAAGMPGAASNAFPAMADENMRHVFEREIAKLQQHQQQQQAAQAQAQFPNFSSLMALQQQVLNGAQDLSLAAAAAKDIKLNGQRSSLEHSAGSSSCSKDGERDDAYPSSLHGRKSEGGGTPAPPAPPSGPGTGAGAPPTAAPPTGGASSNSAAPSPLSNSILPPALSSQGEEFAATASPLQRMASITNSLITQPPVTPHHSTPQRPTKAVLPPITQQQFDMFNNLNTEDIVRRVKEALSQYSISQRLFGESVLGLSQGSVSDLLARPKPWHMLTQKGREPFIRMKMFLEDENAVHKLVASQYKIAPEKLMRTGSYSGSPQMPQGLASKMQAASLPMQKMMSELKLQEPAQAQHLMQQMQAAAMSAAMQQQQVAQAQQQAQQAQQAQQHLQQQAQQHLQQQQHLAQQQHPHQQHHQAAAAAAALHHQSMLLTSPGLPPQHAISLPPSAGGAQPGGPGGNQGSSNPSNSEKKPMLMPVHGTNAMRSLHQHMSPTVYEMAALTQDLDTHDITTKIKEALLANNIGQKIFGEAVLGLSQGSVSELLSKPKPWHMLSIKGREPFIRMQLWLSDANNVERLQLLKNERREASKRRRSTGPNQQDNSSDTSSNDTNDFYTSSPGPGSVGSGVGGAPPSKKQRVLFSEEQKEALRLAFALDPYPNVGTIEFLANELGLATRTITNWFHNHRMRLKQQVPHGPAGQDNPIPSRESTSATPFDPVQFRILLQQRLLELHKERMGMSGAPIPYPPYFAAAAILGRSLAGIPGAAAAAGAAAAAAAVGASGGDELQALNQAFKEQMSGLDLSMPTLKRERSDDYQDDLELEGGGHNLSDNESLEGQEPEDKTTDYEKVLHKSALAAAAAYMSNAVRSSRRKPAAPQWVNPAGAVTNPSAVVAAVAAAAAAAADNERIINGVCVMQASEYGRDDTDSNKPTDGGNDSDHEHAQLEIDQRFMEPEVHIKQEEDDDEEQSGSVNLDNEDNATSEQKLKVINEEKLRMVRVRRLSSTGGGSSEEMPAPLAPPPPPPAASSSIVSGESTTSSSSSSNTSSSTPAVTTAAATAAAGWNY

Target ORF information:

RefSeq Version	NM_001144702.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cut (ct), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144702.2

ORF Insert Sequence:

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGATGCCAA TACACGACAT CCATCCAATC ACCCAACAAC CAACCCCCCT CGACAAAGTA 
ATACTCCCAC TTCATTGCTC TCATTCAGAA TTCCGCGCAA CCCTTGCCGA AAAGGAAGTG 
AACACACTCA AAGAGCAGCT ATCCACCGGC AATCCCGACA GCAACCTCAA CAGCGAAAAC 
AGCGATACAG CAGCAGCGGC AGCAACAGCA GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA 
ACAGCAACAA ACGACATCGA GGACGAGCAG CAGCAACAGT TGCAGCAGAC GGCCAGCGGC 
GGCATTTTGG AAAGCGACAG CGACAAGCTG CTGAACAGCT CCATTGTTGC AGCGGCCATA 
ACGCTGCAGC AGCAGAACGG CAGCAATCTG CTGGCCAACA CAAATACGCC GTCGCCCTCG 
CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC 
GGTGTTGGCG GCGCCTGCCT CAATCCCAAG CTTTTCTTCA ATCACGCCCA ACAGATGATG 
ATGATGGAGG CGGCAGCCGC GGCAGCAGCG GCGGCATTGC AACAGCAGCA GCAACAGCAA 
TCACCACTCC ATTCGCCGGC GAATGAAGTT GCGATTCCCA CAGAACAGCC AGCGGCAACA 
GTGGCAACAG GAGCCGCTGC TGCAGCAGCC GCAGCAGCAA CACCGATTGC AACTGGCAAC 
GTCAAAAGCG GCAGCACCAC CAGCAACGCC AATCACACCA ACAGCAACAA TAGTCACCAG 
GACGAGGAGG AGCTGGACGA TGAGGAGGAG GACGAGGAGG AGGATGAGGA CGAGGATGAT 
GAGGAGGAGA ATGCCTCGAT GCAATCGAAT GCTGATGATA TGGAGCTGGA TGCGCAGCAA 
GAAACCAGAA CTGAGCCAAG TGCAACAACA CAACAGCAGC ATCAGCAGCA GGATACAGAG 
GATCTTGAGG AGAACAAGGA TGCGGGGGAG GCTAGTTTAA ATGTTAGCAA TAATCACAAT 
ACAACCGATA GCAATAATAG TTGTAGTCGA AAGAACAACA ATGGCGGCAA TGAAAGTGAG 
CAACATGTGG CCAGTTCGGC GGAGGACGAT GATTGCGCCA ACAACAATAC AAATACCAGC 
AATAACAACA ATACCAGCAA CACCGCCACC AGCAACACGA ACAACAACAA TAATAATAAC 
AGCAGCAGCG GCAACAGCGA GAAGCGCAAG AAGAAGAACA ATAACAACAA CAACGGCCAG 
CCTGCTGTTC TATTAGCTGC CAAAGATAAA GAGATTAAAG CCCTTTTGGA CGAACTGCAG 
CGCTTGAGGG CGCAGGAGCA GACGCACCTG GTGCAAATCC AACGATTGGA GGAACATCTT 
GAGGTCAAGC GTCAGCACAT CATCCGATTG GAGGCACGTC TGGATAAGCA GCAAATTAAT 
GAGGCACTGG CCGAGGCGAC CGCTTTGTCC GCTGCCGCGT CAACCAACAA CAACAATAAT 
AGCCAGAGCA GCGACAACAA TAAGAAGCTG AATACCGCTG CAGAAAGACC GATGGATGCG 
TCATCCAATG CCGATCTACC GGAGTCCACA AAAGCTCCAG TTCCGGCGGA GGATGACGAA 
GAAGATGAGG ATCAGGCCAT GCTGGTGGAT TCCGAGGAAG CGGAGGACAA GCCAGAGGAC 
TCACATCACG ATGACGACGA AGATGAGGAC GAGGATCGGG AGGCGGTCAA TGCCACCACA 
ACCGATAGCA ACGAGCTCAA AATCAAAAAG GAACAGCACA GTCCGCTGGA TCTGAATGTG 
CTCAGTCCGA ATTCGGCCAT TGCCGCTGCA GCTGCGGCCG CCGCTGCCGC CGCCTGCGCC 
AATGACCCCA ACAAATTCCA GGCTCTGCTC ATCGAGCGGA CCAAAGCCTT GGCCGCAGAG 
GCGCTCAAGA ATGGCGCCAG CGATGCCCTC AGCGAAGAAA CCAACCGAAA TCATGAAGAC 
GAAGACGAAG TGTCGCCAGC AGCAACTGCA ACTGCAACAC TCGCAACGCC TGCAACAGTG 
GCAACACCAA TTGAAACACT GGCCACCGAT TCGGAAGCAA AAGCCAGCAA TGACATGGCG 
CCCAAGCAGG AAATCGAAGA AGTGGATGAC GAGGATGCCA CCGATGAACC GGTGATCCTG 
CCGGAGCAAC TAGTCAACGA CTATTGGCGA CGGGGCTTTG TCGAATCGGG GAATAGCACT 
AATACGAGCA CCGCTGCAAT GCCAGGGAGC AAAACGCCAA CAATTTATCA AAATTTTAAG 
GTCGAGCAGC AGGCACAGTT GCTGCCGCCA CCGCCGCCGC CACCACCACC CCCGCAGCAA 
CAACATCAAC AGCAGCAACA ACAACAACAT CTACACTTTT CACAGCAACA GCAACAACAA 
CATCAGCAGC AACATGGACC ATCGTCCACA GCCCTGCTTA GTCAACTGGT TAACTCAACA 
TTTTCCAACT CTTCCAACTC TTCTCGCCTA GATGCCCACC ATCAGCAGCA GCAGCACCAC 
CAACAGCAGC ACCAACACCA GCAACAACAC CACCAGCAAC AGCACCTCCA CCAGCAACAT 
CACCATCACC TGCAACAGCA ACCAAACAGC GGCAGCAACA GCAATCCAGC GAGCAACGAC 
CACCATCATG GACACCATTT GCATGGCCAT GGTTTGCTGC ATCCTTCGTC GGCGCACCAT 
CTGCACCACC AAACGACCGA ATCCAATTCG AATTCGAGTA CACCCACAGC GGCGGGAAAT 
AACAATGGTA GCAATAACAG CTCCAGCAAC ACAAATGCCA ATAGTACCGC TCAATTGGCC 
GCCAGTTTGG CCAGTACACT CAATGGCACC AAGTCCCTGA TGCAGGAGGA TAGCAATGGC 
TTGGCCGCCG TCGCCATGGC GGCTCATGCC CAACATGCGG CTGCCTTGGG ACCTGGATTT 
TTGCCCGGCC TTCCAGCGTT TCAGTTTGCC GCCGCCCAAG TGGCCGCCGG TGGCGATGGA 
CGGGGTCATT ATCGTTTCGC CGACTCGGAA CTGCAATTGC CACCGGGTGC TTCGATGGCC 
GGTCGTCTTG GAGAGTCGCT CATACCCAAG GGTGATCCCA TGGAGGCCAA ACTCCAAGAG 
ATGTTGCGCT ACAACATGGA CAAGTACGCC AATCAGGCGC TGGATACCCT GCACATTTCG 
CGACGTGTCC GCGAACTGCT ATCGGTGCAC AACATTGGCC AGCGACTATT TGCCAAATAC 
ATACTGGGCC TGTCGCAGGG CACCGTTTCC GAGCTGCTGA GCAAACCCAA GCCGTGGGAC 
AAGTTGACGG AGAAGGGACG AGATAGCTAC CGCAAAATGC ACGCCTGGGC CTGTGACGAT 
AATGCCGTCA TGCTGCTCAA ATCGCTGATA CCCAAGAAAG ATTCTGGACT GCCGCAGTAT 
GCGGGTCGTG GTGCCGGCGG CGCTGGCGGC GATGATTCCA TGTCCGAGGA TCGGATTGCA 
CATATCCTCA GCGAAGCCTC CTCGCTGATG AAACAGAGCA GTGTGGCCCA GCATCGGGAG 
CAGGAGCGTC GCAGTCACGG CGGCGAGGAT TCACACAGCA ACGAGGACAG CAAATCGCCA 
CCTCAAAGCT GCACGTCGCC GTTCTTCAAG GTGGAGAATC AGCTGAAGCA GCACCAGCAT 
CTTAATCCCG AGCAGGCTGC CGCTCAGCAA AGGGAAAGGG AACGCGAGCA AAGAGAACGG 
GAGCAGCAGC AGCGGCTGCG GCACGACGAC CAGGACAAGA TGGCTCGTCT CTACCAGGAG 
CTGATTGCAC GCACTCCGCG TGAGACGGCT TTTCCAAGCT TTCTCTTCTC ACCCTCGCTA 
TTTGGAGGAG CCGCCGGAAT GCCAGGAGCA GCCAGCAATG CCTTTCCCGC CATGGCCGAT 
GAAAATATGC GTCACGTCTT TGAACGTGAG ATTGCCAAGC TGCAGCAGCA CCAACAGCAA 
CAACAAGCTG CCCAGGCTCA GGCACAGTTC CCCAACTTCT CGAGCCTGAT GGCTTTGCAG 
CAGCAGGTCC TCAACGGAGC CCAGGATCTC TCGCTAGCTG CCGCTGCAGC CAAGGACATC 
AAGTTGAACG GTCAGCGATC CTCGTTGGAG CACAGTGCCG GCAGCAGCAG TTGTTCAAAA 
GATGGCGAAC GAGATGATGC GTATCCAAGT TCCCTACATG GTCGCAAGTC CGAGGGAGGT 
GGCACACCCG CACCACCTGC CCCACCATCT GGCCCAGGAA CCGGAGCAGG AGCCCCACCC 
ACAGCAGCAC CACCAACCGG CGGTGCCAGC AGCAATTCGG CAGCACCCAG TCCGCTAAGC 
AATTCAATAC TTCCACCGGC ATTGAGCAGC CAAGGTGAGG AGTTCGCCGC GACAGCAAGT 
CCTTTGCAGC GAATGGCCTC GATCACCAAT TCGCTGATTA CCCAACCACC GGTGACGCCG 
CACCACAGTA CGCCACAGCG ACCCACCAAG GCGGTTCTGC CACCGATCAC CCAGCAACAG 
TTCGATATGT TCAACAACCT TAACACCGAG GATATAGTCA GACGTGTGAA GGAGGCCCTG 
TCGCAGTACT CCATCTCCCA GCGATTGTTC GGCGAGTCCG TGCTGGGTTT GTCTCAGGGA 
TCGGTCTCCG ATTTATTGGC TAGACCCAAG CCATGGCACA TGCTCACTCA AAAGGGCCGT 
GAACCCTTCA TTCGCATGAA GATGTTCCTG GAGGATGAGA ACGCGGTGCA CAAACTGGTG 
GCCAGTCAAT ACAAGATTGC CCCCGAGAAG CTAATGCGAA CGGGCAGTTA CAGTGGAAGT 
CCGCAGATGC CACAGGGATT GGCAAGCAAA ATGCAGGCTG CCTCCCTGCC CATGCAGAAG 
ATGATGAGCG AACTTAAGCT GCAGGAGCCA GCACAGGCGC AGCACTTGAT GCAGCAAATG 
CAGGCGGCGG CAATGTCGGC AGCAATGCAA CAGCAGCAAG TGGCACAAGC GCAGCAGCAG 
GCTCAGCAGG CGCAACAGGC ACAACAGCAT TTGCAGCAGC AGGCGCAGCA GCACTTGCAA 
CAGCAGCAAC ATCTTGCCCA GCAGCAACAC CCCCATCAGC AGCACCATCA AGCGGCCGCC 
GCGGCTGCCG CATTGCACCA CCAAAGCATG CTTCTGACCT CGCCCGGACT GCCACCCCAG 
CATGCCATCA GTCTGCCACC TTCAGCGGGT GGAGCCCAAC CAGGTGGTCC TGGTGGCAAT 
CAGGGCAGCT CAAATCCCTC AAACAGCGAA AAGAAACCCA TGCTGATGCC GGTTCATGGC 
ACTAATGCCA TGAGAAGTCT GCACCAGCAC ATGTCGCCCA CCGTTTACGA AATGGCCGCT 
TTAACCCAAG ATCTGGACAC CCACGATATC ACTACCAAGA TCAAGGAGGC GTTGTTGGCC 
AACAATATTG GTCAGAAAAT ATTTGGCGAA GCCGTTTTGG GACTTTCGCA GGGATCCGTA 
TCTGAGCTGC TGAGCAAACC GAAGCCCTGG CACATGCTCT CCATCAAGGG CAGGGAACCC 
TTCATCCGGA TGCAATTGTG GCTCAGTGAT GCCAACAATG TTGAACGATT GCAGTTGCTG 
AAAAATGAGC GACGGGAGGC CAGCAAACGA AGGAGGAGCA CAGGCCCAAA CCAACAGGAC 
AATAGTAGCG ATACCTCCAG CAATGATACC AATGACTTCT ATACCAGCAG TCCGGGTCCA 
GGATCCGTCG GTTCGGGTGT CGGTGGTGCT CCGCCGAGCA AAAAACAGCG TGTCCTCTTC 
TCCGAGGAGC AAAAGGAGGC ACTTCGGCTG GCATTCGCCT TGGATCCGTA TCCAAATGTG 
GGGACCATCG AGTTTCTGGC CAATGAGCTG GGCTTGGCAA CGCGGACGAT CACCAACTGG 
TTCCACAATC ATCGCATGCG ATTGAAGCAA CAGGTGCCAC ACGGACCTGC CGGTCAAGAT 
AATCCGATAC CGAGTCGCGA GAGTACCAGT GCCACGCCCT TCGATCCCGT CCAATTCCGC 
ATTCTGCTGC AGCAGCGTCT ACTGGAGCTC CACAAGGAGC GAATGGGCAT GAGCGGTGCC 
CCCATTCCCT ATCCGCCGTA CTTTGCTGCC GCTGCCATTT TGGGTCGCAG TCTGGCGGGG 
ATTCCCGGTG CGGCAGCGGC CGCTGGAGCA GCTGCAGCAG CCGCCGCAGT TGGCGCCTCT 
GGTGGTGATG AGTTGCAGGC TCTGAATCAG GCCTTCAAGG AGCAGATGAG CGGTTTGGAT 
CTGTCGATGC CCACCTTAAA GCGGGAACGC AGCGATGACT ATCAGGACGA TCTGGAACTG 
GAGGGTGGTG GTCACAATCT AAGTGACAAC GAATCGCTGG AGGGTCAAGA GCCGGAGGAC 
AAGACCACCG ATTACGAGAA GGTGCTGCAC AAATCCGCTT TGGCCGCTGC CGCCGCTTAC 
ATGTCCAATG CAGTGCGGAG TTCACGACGA AAGCCAGCTG CCCCACAGTG GGTCAATCCC 
GCCGGAGCGG TGACCAATCC AAGTGCTGTA GTTGCAGCCG TTGCTGCTGC CGCAGCTGCC 
GCGGCGGACA ATGAACGCAT CATCAACGGG GTGTGCGTGA TGCAGGCCTC CGAGTACGGC 
CGCGATGATA CTGATAGCAA CAAGCCAACG GACGGCGGCA ACGATTCCGA TCATGAGCAC 
GCCCAGCTGG AGATCGATCA GCGTTTCATG GAACCGGAGG TGCACATCAA ACAGGAGGAG 
GACGACGATG AGGAGCAATC GGGATCGGTT AATCTGGACA ACGAGGACAA TGCGACTAGC 
GAGCAAAAAC TGAAGGTGAT CAACGAGGAG AAGCTGCGAA TGGTGCGAGT TCGCCGGCTG 
AGCAGCACTG GTGGTGGTTC CTCTGAGGAA ATGCCAGCTC CCCTCGCACC ACCGCCTCCT 
CCGCCAGCAG CTTCTTCTTC TATCGTTAGT GGAGAAAGCA CCACCAGCAG CAGTAGTAGC 
AGCAACACCA GCAGCAGCAC ACCGGCGGTA ACCACTGCAG CAGCAACTGC GGCAGCCGGT 
TGGAACTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ct ( NM_080025.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524764.1 Drosophila melanogaster cut (ct), transcript variant A, mRNA.

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CloneID	OFa02348
Clone ID Related Accession (Same CDS sequence)	NM_080025.2
Accession Version	NM_080025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cut, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_080025.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGCGCGCAA CCCTTGCCGA AAAGGAAGTG AACACACTCA AAGAGCAGCT ATCCACCGGC 
AATCCCGACA GCAACCTCAA CAGCGAAAAC AGCGATACAG CAGCAGCGGC AGCAACAGCA 
GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA ACAGCAACAA ACGACATCGA GGACGAGCAG 
CAGCAACAGT TGCAGCAGAC GGCCAGCGGC GGCATTTTGG AAAGCGACAG CGACAAGCTG 
CTGAACAGCT CCATTGTTGC AGCGGCCATA ACGCTGCAGC AGCAGAACGG CAGCAATCTG 
CTGGCCAACA CAAATACGCC GTCGCCCTCG CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG 
CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC GGTGTTGGCG GCGCCTGCCT CAATCCCAAG 
CTTTTCTTCA ATCACGCCCA ACAGATGATG ATGATGGAGG CGGCAGCCGC GGCAGCAGCG 
GCGGCATTGC AACAGCAGCA GCAACAGCAA TCACCACTCC ATTCGCCGGC GAATGAAGTT 
GCGATTCCCA CAGAACAGCC AGCGGCAACA GTGGCAACAG GAGCCGCTGC TGCAGCAGCC 
GCAGCAGCAA CACCGATTGC AACTGGCAAC GTCAAAAGCG GCAGCACCAC CAGCAACGCC 
AATCACACCA ACAGCAACAA TAGTCACCAG GACGAGGAGG AGCTGGACGA TGAGGAGGAG 
GACGAGGAGG AGGATGAGGA CGAGGATGAT GAGGAGGAGA ATGCCTCGAT GCAATCGAAT 
GCTGATGATA TGGAGCTGGA TGCGCAGCAA GAAACCAGAA CTGAGCCAAG TGCAACAACA 
CAACAGCAGC ATCAGCAGCA GGATACAGAG GATCTTGAGG AGAACAAGGA TGCGGGGGAG 
GCTAGTTTAA ATGTTAGCAA TAATCACAAT ACAACCGATA GCAATAATAG TTGTAGTCGA 
AAGAACAACA ATGGCGGCAA TGAAAGTGAG CAACATGTGG CCAGTTCGGC GGAGGACGAT 
GATTGCGCCA ACAACAATAC AAATACCAGC AATAACAACA ATACCAGCAA CACCGCCACC 
AGCAACACGA ACAACAACAA TAATAATAAC AGCAGCAGCG GCAACAGCGA GAAGCGCAAG 
AAGAAGAACA ATAACAACAA CAACGGCCAG CCTGCTGTTC TATTAGCTGC CAAAGATAAA 
GAGATTAAAG CCCTTTTGGA CGAACTGCAG CGCTTGAGGG CGCAGGAGCA GACGCACCTG 
GTGCAAATCC AACGATTGGA GGAACATCTT GAGGTCAAGC GTCAGCACAT CATCCGATTG 
GAGGCACGTC TGGATAAGCA GCAAATTAAT GAGGCACTGG CCGAGGCGAC CGCTTTGTCC 
GCTGCCGCGT CAACCAACAA CAACAATAAT AGCCAGAGCA GCGACAACAA TAAGAAGCTG 
AATACCGCTG CAGAAAGACC GATGGATGCG TCATCCAATG CCGATCTACC GGAGTCCACA 
AAAGCTCCAG TTCCGGCGGA GGATGACGAA GAAGATGAGG ATCAGGCCAT GCTGGTGGAT 
TCCGAGGAAG CGGAGGACAA GCCAGAGGAC TCACATCACG ATGACGACGA AGATGAGGAC 
GAGGATCGGG AGGCGGTCAA TGCCACCACA ACCGATAGCA ACGAGCTCAA AATCAAAAAG 
GAACAGCACA GTCCGCTGGA TCTGAATGTG CTCAGTCCGA ATTCGGCCAT TGCCGCTGCA 
GCTGCGGCCG CCGCTGCCGC CGCCTGCGCC AATGACCCCA ACAAATTCCA GGCTCTGCTC 
ATCGAGCGGA CCAAAGCCTT GGCCGCAGAG GCGCTCAAGA ATGGCGCCAG CGATGCCCTC 
AGCGAAGATG CCCACCATCA GCAGCAGCAG CACCACCAAC AGCAGCACCA ACACCAGCAA 
CAACACCACC AGCAACAGCA CCTCCACCAG CAACATCACC ATCACCTGCA ACAGCAACCA 
AACAGCGGCA GCAACAGCAA TCCAGCGAGC AACGACCACC ATCATGGACA CCATTTGCAT 
GGCCATGGTT TGCTGCATCC TTCGTCGGCG CACCATCTGC ACCACCAAAC GACCGAATCC 
AATTCGAATT CGAGTACACC CACAGCGGCG GGAAATAACA ATGGTAGCAA TAACAGCTCC 
AGCAACACAA ATGCCAATAG TACCGCTCAA TTGGCCGCCA GTTTGGCCAG TACACTCAAT 
GGCACCAAGT CCCTGATGCA GGAGGATAGC AATGGCTTGG CCGCCGTCGC CATGGCGGCT 
CATGCCCAAC ATGCGGCTGC CTTGGGACCT GGATTTTTGC CCGGCCTTCC AGCGTTTCAG 
TTTGCCGCCG CCCAAGTGGC CGCCGGTGGC GATGGACGGG GTCATTATCG TTTCGCCGAC 
TCGGAACTGC AATTGCCACC GGGTGCTTCG ATGGCCGGTC GTCTTGGAGA GTCGCTCATA 
CCCAAGGGTG ATCCCATGGA GGCCAAACTC CAAGAGATGT TGCGCTACAA CATGGACAAG 
TACGCCAATC AGGCGCTGGA TACCCTGCAC ATTTCGCGAC GTGTCCGCGA ACTGCTATCG 
GTGCACAACA TTGGCCAGCG ACTATTTGCC AAATACATAC TGGGCCTGTC GCAGGGCACC 
GTTTCCGAGC TGCTGAGCAA ACCCAAGCCG TGGGACAAGT TGACGGAGAA GGGACGAGAT 
AGCTACCGCA AAATGCACGC CTGGGCCTGT GACGATAATG CCGTCATGCT GCTCAAATCG 
CTGATACCCA AGAAAGATTC TGGACTGCCG CAGTATGCGG GTCGTGGTGC CGGCGGCGCT 
GGCGGCGATG ATTCCATGTC CGAGGATCGG ATTGCACATA TCCTCAGCGA AGCCTCCTCG 
CTGATGAAAC AGAGCAGTGT GGCCCAGCAT CGGGAGCAGG AGCGTCGCAG TCACGGCGGC 
GAGGATTCAC ACAGCAACGA GGACAGCAAA TCGCCACCTC AAAGCTGCAC GTCGCCGTTC 
TTCAAGGTGG AGAATCAGCT GAAGCAGCAC CAGCATCTTA ATCCCGAGCA GGCTGCCGCT 
CAGCAAAGGG AAAGGGAACG CGAGCAAAGA GAACGGGAGC AGCAGCAGCG GCTGCGGCAC 
GACGACCAGG ACAAGATGGC TCGTCTCTAC CAGGAGCTGA TTGCACGCAC TCCGCGTGAG 
ACGGCTTTTC CAAGCTTTCT CTTCTCACCC TCGCTATTTG GAGGAGCCGC CGGAATGCCA 
GGAGCAGCCA GCAATGCCTT TCCCGCCATG GCCGATGAAA ATATGCGTCA CGTCTTTGAA 
CGTGAGATTG CCAAGCTGCA GCAGCACCAA CAGCAACAAC AAGCTGCCCA GGCTCAGGCA 
CAGTTCCCCA ACTTCTCGAG CCTGATGGCT TTGCAGCAGC AGGTCCTCAA CGGAGCCCAG 
GATCTCTCGC TAGCTGCCGC TGCAGCCAAG GACATCAAGT TGAACGGTCA GCGATCCTCG 
TTGGAGCACA GTGCCGGCAG CAGCAGTTGT TCAAAAGATG GCGAACGAGA TGATGCGTAT 
CCAAGTTCCC TACATGGTCG CAAGTCCGAG GGAGGTGGCA CACCCGCACC ACCTGCCCCA 
CCATCTGGCC CAGGAACCGG AGCAGGAGCC CCACCCACAG CAGCACCACC AACCGGCGGT 
GCCAGCAGCA ATTCGGCAGC ACCCAGTCCG CTAAGCAATT CAATACTTCC ACCGGCATTG 
AGCAGCCAAG GTGAGGAGTT CGCCGCGACA GCAAGTCCTT TGCAGCGAAT GGCCTCGATC 
ACCAATTCGC TGATTACCCA ACCACCGGTG ACGCCGCACC ACAGTACGCC ACAGCGACCC 
ACCAAGGCGG TTCTGCCACC GATCACCCAG CAACAGTTCG ATATGTTCAA CAACCTTAAC 
ACCGAGGATA TAGTCAGACG TGTGAAGGAG GCCCTGTCGC AGTACTCCAT CTCCCAGCGA 
TTGTTCGGCG AGTCCGTGCT GGGTTTGTCT CAGGGATCGG TCTCCGATTT ATTGGCTAGA 
CCCAAGCCAT GGCACATGCT CACTCAAAAG GGCCGTGAAC CCTTCATTCG CATGAAGATG 
TTCCTGGAGG ATGAGAACGC GGTGCACAAA CTGGTGGCCA GTCAATACAA GATTGCCCCC 
GAGAAGCTAA TGCGAACGGG CAGTTACAGT GGAAGTCCGC AGATGCCACA GGGATTGGCA 
AGCAAAATGC AGGCTGCCTC CCTGCCCATG CAGAAGATGA TGAGCGAACT TAAGCTGCAG 
GAGCCAGCAC AGGCGCAGCA CTTGATGCAG CAAATGCAGG CGGCGGCAAT GTCGGCAGCA 
ATGCAACAGC AGCAAGTGGC ACAAGCGCAG CAGCAGGCTC AGCAGGCGCA ACAGGCACAA 
CAGCATTTGC AGCAGCAGGC GCAGCAGCAC TTGCAACAGC AGCAACATCT TGCCCAGCAG 
CAACACCCCC ATCAGCAGCA CCATCAAGCG GCCGCCGCGG CTGCCGCATT GCACCACCAA 
AGCATGCTTC TGACCTCGCC CGGACTGCCA CCCCAGCATG CCATCAGTCT GCCACCTTCA 
GCGGGTGGAG CCCAACCAGG TGGTCCTGGT GGCAATCAGG GCAGCTCAAA TCCCTCAAAC 
AGCGAAAAGA AACCCATGCT GATGCCGGTT CATGGCACTA ATGCCATGAG AAGTCTGCAC 
CAGCACATGT CGCCCACCGT TTACGAAATG GCCGCTTTAA CCCAAGATCT GGACACCCAC 
GATATCACTA CCAAGATCAA GGAGGCGTTG TTGGCCAACA ATATTGGTCA GAAAATATTT 
GGCGAAGCCG TTTTGGGACT TTCGCAGGGA TCCGTATCTG AGCTGCTGAG CAAACCGAAG 
CCCTGGCACA TGCTCTCCAT CAAGGGCAGG GAACCCTTCA TCCGGATGCA ATTGTGGCTC 
AGTGATGCCA ACAATGTTGA ACGATTGCAG TTGCTGAAAA ATGAGCGACG GGAGGCCAGC 
AAACGAAGGA GGAGCACAGG CCCAAACCAA CAGGACAATA GTAGCGATAC CTCCAGCAAT 
GATACCAATG ACTTCTATAC CAGCAGTCCG GGTCCAGGAT CCGTCGGTTC GGGTGTCGGT 
GGTGCTCCGC CGAGCAAAAA ACAGCGTGTC CTCTTCTCCG AGGAGCAAAA GGAGGCACTT 
CGGCTGGCAT TCGCCTTGGA TCCGTATCCA AATGTGGGGA CCATCGAGTT TCTGGCCAAT 
GAGCTGGGCT TGGCAACGCG GACGATCACC AACTGGTTCC ACAATCATCG CATGCGATTG 
AAGCAACAGG TGCCACACGG ACCTGCCGGT CAAGATAATC CGATACCGAG TCGCGAGAGT 
ACCAGTGCCA CGCCCTTCGA TCCCGTCCAA TTCCGCATTC TGCTGCAGCA GCGTCTACTG 
GAGCTCCACA AGGAGCGAAT GGGCATGAGC GGTGCCCCCA TTCCCTATCC GCCGTACTTT 
GCTGCCGCTG CCATTTTGGG TCGCAGTCTG GCGGGGATTC CCGGTGCGGC AGCGGCCGCT 
GGAGCAGCTG CAGCAGCCGC CGCAGTTGGC GCCTCTGGTG GTGATGAGTT GCAGGCTCTG 
AATCAGGCCT TCAAGGAGCA GATGAGCGGT TTGGATCTGT CGATGCCCAC CTTAAAGCGG 
GAACGCAGCG ATGACTATCA GGACGATCTG GAACTGGAGG GTGGTGGTCA CAATCTAAGT 
GACAACGAAT CGCTGGAGGG TCAAGAGCCG GAGGACAAGA CCACCGATTA CGAGAAGGTG 
CTGCACAAAT CCGCTTTGGC CGCTGCCGCC GCTTACATGT CCAATGCAGT GCGGAGTTCA 
CGACGAAAGC CAGCTGCCCC ACAGTGGGTC AATCCCGCCG GAGCGGTGAC CAATCCAAGT 
GCTGTAGTTG CAGCCGTTGC TGCTGCCGCA GCTGCCGCGG CGGACAATGA ACGCATCATC 
AACGGGGTGT GCGTGATGCA GGCCTCCGAG TACGGCCGCG ATGATACTGA TAGCAACAAG 
CCAACGGACG GCGGCAACGA TTCCGATCAT GAGCACGCCC AGCTGGAGAT CGATCAGCGT 
TTCATGGAAC CGGAGGTGCA CATCAAACAG GAGGAGGACG ACGATGAGGA GCAATCGGGA 
TCGGTTAATC TGGACAACGA GGACAATGCG ACTAGCGAGC AAAAACTGAA GGTGATCAAC 
GAGGAGAAGC TGCGAATGGT GCGAGTTCGC CGGCTGAGCA GCACTGGTGG TGGTTCCTCT 
GAGGAAATGC CAGCTCCCCT CGCACCACCG CCTCCTCCGC CAGCAGCTTC TTCTTCTATC 
GTTAGTGGAG AAAGCACCAC CAGCAGCAGT AGTAGCAGCA ACACCAGCAG CAGCACACCG 
GCGGTAACCA CTGCAGCAGC AACTGCGGCA GCCGGTTGGA ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524764.1
CDS	269..6796
Translation	MQPTLPQAAGTADMDLTAVQSINDWFFKKEQIYLLAQFWQQRATLAEKEVNTLKEQLSTGNPDSNLNSENSDTAAAAATAAAVAAVVAGATATNDIEDEQQQQLQQTASGGILESDSDKLLNSSIVAAAITLQQQNGSNLLANTNTPSPSPPLLSAEQQQQLQSSLQQSGGVGGACLNPKLFFNHAQQMMMMEAAAAAAAAALQQQQQQQSPLHSPANEVAIPTEQPAATVATGAAAAAAAAATPIATGNVKSGSTTSNANHTNSNNSHQDEEELDDEEEDEEEDEDEDDEEENASMQSNADDMELDAQQETRTEPSATTQQQHQQQDTEDLEENKDAGEASLNVSNNHNTTDSNNSCSRKNNNGGNESEQHVASSAEDDDCANNNTNTSNNNNTSNTATSNTNNNNNNNSSSGNSEKRKKKNNNNNNGQPAVLLAAKDKEIKALLDELQRLRAQEQTHLVQIQRLEEHLEVKRQHIIRLEARLDKQQINEALAEATALSAAASTNNNNNSQSSDNNKKLNTAAERPMDASSNADLPESTKAPVPAEDDEEDEDQAMLVDSEEAEDKPEDSHHDDDEDEDEDREAVNATTTDSNELKIKKEQHSPLDLNVLSPNSAIAAAAAAAAAAACANDPNKFQALLIERTKALAAEALKNGASDALSEDAHHQQQQHHQQQHQHQQQHHQQQHLHQQHHHHLQQQPNSGSNSNPASNDHHHGHHLHGHGLLHPSSAHHLHHQTTESNSNSSTPTAAGNNNGSNNSSSNTNANSTAQLAASLASTLNGTKSLMQEDSNGLAAVAMAAHAQHAAALGPGFLPGLPAFQFAAAQVAAGGDGRGHYRFADSELQLPPGASMAGRLGESLIPKGDPMEAKLQEMLRYNMDKYANQALDTLHISRRVRELLSVHNIGQRLFAKYILGLSQGTVSELLSKPKPWDKLTEKGRDSYRKMHAWACDDNAVMLLKSLIPKKDSGLPQYAGRGAGGAGGDDSMSEDRIAHILSEASSLMKQSSVAQHREQERRSHGGEDSHSNEDSKSPPQSCTSPFFKVENQLKQHQHLNPEQAAAQQREREREQREREQQQRLRHDDQDKMARLYQELIARTPRETAFPSFLFSPSLFGGAAGMPGAASNAFPAMADENMRHVFEREIAKLQQHQQQQQAAQAQAQFPNFSSLMALQQQVLNGAQDLSLAAAAAKDIKLNGQRSSLEHSAGSSSCSKDGERDDAYPSSLHGRKSEGGGTPAPPAPPSGPGTGAGAPPTAAPPTGGASSNSAAPSPLSNSILPPALSSQGEEFAATASPLQRMASITNSLITQPPVTPHHSTPQRPTKAVLPPITQQQFDMFNNLNTEDIVRRVKEALSQYSISQRLFGESVLGLSQGSVSDLLARPKPWHMLTQKGREPFIRMKMFLEDENAVHKLVASQYKIAPEKLMRTGSYSGSPQMPQGLASKMQAASLPMQKMMSELKLQEPAQAQHLMQQMQAAAMSAAMQQQQVAQAQQQAQQAQQAQQHLQQQAQQHLQQQQHLAQQQHPHQQHHQAAAAAAALHHQSMLLTSPGLPPQHAISLPPSAGGAQPGGPGGNQGSSNPSNSEKKPMLMPVHGTNAMRSLHQHMSPTVYEMAALTQDLDTHDITTKIKEALLANNIGQKIFGEAVLGLSQGSVSELLSKPKPWHMLSIKGREPFIRMQLWLSDANNVERLQLLKNERREASKRRRSTGPNQQDNSSDTSSNDTNDFYTSSPGPGSVGSGVGGAPPSKKQRVLFSEEQKEALRLAFALDPYPNVGTIEFLANELGLATRTITNWFHNHRMRLKQQVPHGPAGQDNPIPSRESTSATPFDPVQFRILLQQRLLELHKERMGMSGAPIPYPPYFAAAAILGRSLAGIPGAAAAAGAAAAAAAVGASGGDELQALNQAFKEQMSGLDLSMPTLKRERSDDYQDDLELEGGGHNLSDNESLEGQEPEDKTTDYEKVLHKSALAAAAAYMSNAVRSSRRKPAAPQWVNPAGAVTNPSAVVAAVAAAAAAAADNERIINGVCVMQASEYGRDDTDSNKPTDGGNDSDHEHAQLEIDQRFMEPEVHIKQEEDDDEEQSGSVNLDNEDNATSEQKLKVINEEKLRMVRVRRLSSTGGGSSEEMPAPLAPPPPPPAASSSIVSGESTTSSSSSSNTSSSTPAVTTAAATAAAGWNY

Target ORF information:

RefSeq Version	NM_080025.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cut (ct), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080025.2

ORF Insert Sequence:

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGCGCGCAA CCCTTGCCGA AAAGGAAGTG AACACACTCA AAGAGCAGCT ATCCACCGGC 
AATCCCGACA GCAACCTCAA CAGCGAAAAC AGCGATACAG CAGCAGCGGC AGCAACAGCA 
GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA ACAGCAACAA ACGACATCGA GGACGAGCAG 
CAGCAACAGT TGCAGCAGAC GGCCAGCGGC GGCATTTTGG AAAGCGACAG CGACAAGCTG 
CTGAACAGCT CCATTGTTGC AGCGGCCATA ACGCTGCAGC AGCAGAACGG CAGCAATCTG 
CTGGCCAACA CAAATACGCC GTCGCCCTCG CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG 
CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC GGTGTTGGCG GCGCCTGCCT CAATCCCAAG 
CTTTTCTTCA ATCACGCCCA ACAGATGATG ATGATGGAGG CGGCAGCCGC GGCAGCAGCG 
GCGGCATTGC AACAGCAGCA GCAACAGCAA TCACCACTCC ATTCGCCGGC GAATGAAGTT 
GCGATTCCCA CAGAACAGCC AGCGGCAACA GTGGCAACAG GAGCCGCTGC TGCAGCAGCC 
GCAGCAGCAA CACCGATTGC AACTGGCAAC GTCAAAAGCG GCAGCACCAC CAGCAACGCC 
AATCACACCA ACAGCAACAA TAGTCACCAG GACGAGGAGG AGCTGGACGA TGAGGAGGAG 
GACGAGGAGG AGGATGAGGA CGAGGATGAT GAGGAGGAGA ATGCCTCGAT GCAATCGAAT 
GCTGATGATA TGGAGCTGGA TGCGCAGCAA GAAACCAGAA CTGAGCCAAG TGCAACAACA 
CAACAGCAGC ATCAGCAGCA GGATACAGAG GATCTTGAGG AGAACAAGGA TGCGGGGGAG 
GCTAGTTTAA ATGTTAGCAA TAATCACAAT ACAACCGATA GCAATAATAG TTGTAGTCGA 
AAGAACAACA ATGGCGGCAA TGAAAGTGAG CAACATGTGG CCAGTTCGGC GGAGGACGAT 
GATTGCGCCA ACAACAATAC AAATACCAGC AATAACAACA ATACCAGCAA CACCGCCACC 
AGCAACACGA ACAACAACAA TAATAATAAC AGCAGCAGCG GCAACAGCGA GAAGCGCAAG 
AAGAAGAACA ATAACAACAA CAACGGCCAG CCTGCTGTTC TATTAGCTGC CAAAGATAAA 
GAGATTAAAG CCCTTTTGGA CGAACTGCAG CGCTTGAGGG CGCAGGAGCA GACGCACCTG 
GTGCAAATCC AACGATTGGA GGAACATCTT GAGGTCAAGC GTCAGCACAT CATCCGATTG 
GAGGCACGTC TGGATAAGCA GCAAATTAAT GAGGCACTGG CCGAGGCGAC CGCTTTGTCC 
GCTGCCGCGT CAACCAACAA CAACAATAAT AGCCAGAGCA GCGACAACAA TAAGAAGCTG 
AATACCGCTG CAGAAAGACC GATGGATGCG TCATCCAATG CCGATCTACC GGAGTCCACA 
AAAGCTCCAG TTCCGGCGGA GGATGACGAA GAAGATGAGG ATCAGGCCAT GCTGGTGGAT 
TCCGAGGAAG CGGAGGACAA GCCAGAGGAC TCACATCACG ATGACGACGA AGATGAGGAC 
GAGGATCGGG AGGCGGTCAA TGCCACCACA ACCGATAGCA ACGAGCTCAA AATCAAAAAG 
GAACAGCACA GTCCGCTGGA TCTGAATGTG CTCAGTCCGA ATTCGGCCAT TGCCGCTGCA 
GCTGCGGCCG CCGCTGCCGC CGCCTGCGCC AATGACCCCA ACAAATTCCA GGCTCTGCTC 
ATCGAGCGGA CCAAAGCCTT GGCCGCAGAG GCGCTCAAGA ATGGCGCCAG CGATGCCCTC 
AGCGAAGATG CCCACCATCA GCAGCAGCAG CACCACCAAC AGCAGCACCA ACACCAGCAA 
CAACACCACC AGCAACAGCA CCTCCACCAG CAACATCACC ATCACCTGCA ACAGCAACCA 
AACAGCGGCA GCAACAGCAA TCCAGCGAGC AACGACCACC ATCATGGACA CCATTTGCAT 
GGCCATGGTT TGCTGCATCC TTCGTCGGCG CACCATCTGC ACCACCAAAC GACCGAATCC 
AATTCGAATT CGAGTACACC CACAGCGGCG GGAAATAACA ATGGTAGCAA TAACAGCTCC 
AGCAACACAA ATGCCAATAG TACCGCTCAA TTGGCCGCCA GTTTGGCCAG TACACTCAAT 
GGCACCAAGT CCCTGATGCA GGAGGATAGC AATGGCTTGG CCGCCGTCGC CATGGCGGCT 
CATGCCCAAC ATGCGGCTGC CTTGGGACCT GGATTTTTGC CCGGCCTTCC AGCGTTTCAG 
TTTGCCGCCG CCCAAGTGGC CGCCGGTGGC GATGGACGGG GTCATTATCG TTTCGCCGAC 
TCGGAACTGC AATTGCCACC GGGTGCTTCG ATGGCCGGTC GTCTTGGAGA GTCGCTCATA 
CCCAAGGGTG ATCCCATGGA GGCCAAACTC CAAGAGATGT TGCGCTACAA CATGGACAAG 
TACGCCAATC AGGCGCTGGA TACCCTGCAC ATTTCGCGAC GTGTCCGCGA ACTGCTATCG 
GTGCACAACA TTGGCCAGCG ACTATTTGCC AAATACATAC TGGGCCTGTC GCAGGGCACC 
GTTTCCGAGC TGCTGAGCAA ACCCAAGCCG TGGGACAAGT TGACGGAGAA GGGACGAGAT 
AGCTACCGCA AAATGCACGC CTGGGCCTGT GACGATAATG CCGTCATGCT GCTCAAATCG 
CTGATACCCA AGAAAGATTC TGGACTGCCG CAGTATGCGG GTCGTGGTGC CGGCGGCGCT 
GGCGGCGATG ATTCCATGTC CGAGGATCGG ATTGCACATA TCCTCAGCGA AGCCTCCTCG 
CTGATGAAAC AGAGCAGTGT GGCCCAGCAT CGGGAGCAGG AGCGTCGCAG TCACGGCGGC 
GAGGATTCAC ACAGCAACGA GGACAGCAAA TCGCCACCTC AAAGCTGCAC GTCGCCGTTC 
TTCAAGGTGG AGAATCAGCT GAAGCAGCAC CAGCATCTTA ATCCCGAGCA GGCTGCCGCT 
CAGCAAAGGG AAAGGGAACG CGAGCAAAGA GAACGGGAGC AGCAGCAGCG GCTGCGGCAC 
GACGACCAGG ACAAGATGGC TCGTCTCTAC CAGGAGCTGA TTGCACGCAC TCCGCGTGAG 
ACGGCTTTTC CAAGCTTTCT CTTCTCACCC TCGCTATTTG GAGGAGCCGC CGGAATGCCA 
GGAGCAGCCA GCAATGCCTT TCCCGCCATG GCCGATGAAA ATATGCGTCA CGTCTTTGAA 
CGTGAGATTG CCAAGCTGCA GCAGCACCAA CAGCAACAAC AAGCTGCCCA GGCTCAGGCA 
CAGTTCCCCA ACTTCTCGAG CCTGATGGCT TTGCAGCAGC AGGTCCTCAA CGGAGCCCAG 
GATCTCTCGC TAGCTGCCGC TGCAGCCAAG GACATCAAGT TGAACGGTCA GCGATCCTCG 
TTGGAGCACA GTGCCGGCAG CAGCAGTTGT TCAAAAGATG GCGAACGAGA TGATGCGTAT 
CCAAGTTCCC TACATGGTCG CAAGTCCGAG GGAGGTGGCA CACCCGCACC ACCTGCCCCA 
CCATCTGGCC CAGGAACCGG AGCAGGAGCC CCACCCACAG CAGCACCACC AACCGGCGGT 
GCCAGCAGCA ATTCGGCAGC ACCCAGTCCG CTAAGCAATT CAATACTTCC ACCGGCATTG 
AGCAGCCAAG GTGAGGAGTT CGCCGCGACA GCAAGTCCTT TGCAGCGAAT GGCCTCGATC 
ACCAATTCGC TGATTACCCA ACCACCGGTG ACGCCGCACC ACAGTACGCC ACAGCGACCC 
ACCAAGGCGG TTCTGCCACC GATCACCCAG CAACAGTTCG ATATGTTCAA CAACCTTAAC 
ACCGAGGATA TAGTCAGACG TGTGAAGGAG GCCCTGTCGC AGTACTCCAT CTCCCAGCGA 
TTGTTCGGCG AGTCCGTGCT GGGTTTGTCT CAGGGATCGG TCTCCGATTT ATTGGCTAGA 
CCCAAGCCAT GGCACATGCT CACTCAAAAG GGCCGTGAAC CCTTCATTCG CATGAAGATG 
TTCCTGGAGG ATGAGAACGC GGTGCACAAA CTGGTGGCCA GTCAATACAA GATTGCCCCC 
GAGAAGCTAA TGCGAACGGG CAGTTACAGT GGAAGTCCGC AGATGCCACA GGGATTGGCA 
AGCAAAATGC AGGCTGCCTC CCTGCCCATG CAGAAGATGA TGAGCGAACT TAAGCTGCAG 
GAGCCAGCAC AGGCGCAGCA CTTGATGCAG CAAATGCAGG CGGCGGCAAT GTCGGCAGCA 
ATGCAACAGC AGCAAGTGGC ACAAGCGCAG CAGCAGGCTC AGCAGGCGCA ACAGGCACAA 
CAGCATTTGC AGCAGCAGGC GCAGCAGCAC TTGCAACAGC AGCAACATCT TGCCCAGCAG 
CAACACCCCC ATCAGCAGCA CCATCAAGCG GCCGCCGCGG CTGCCGCATT GCACCACCAA 
AGCATGCTTC TGACCTCGCC CGGACTGCCA CCCCAGCATG CCATCAGTCT GCCACCTTCA 
GCGGGTGGAG CCCAACCAGG TGGTCCTGGT GGCAATCAGG GCAGCTCAAA TCCCTCAAAC 
AGCGAAAAGA AACCCATGCT GATGCCGGTT CATGGCACTA ATGCCATGAG AAGTCTGCAC 
CAGCACATGT CGCCCACCGT TTACGAAATG GCCGCTTTAA CCCAAGATCT GGACACCCAC 
GATATCACTA CCAAGATCAA GGAGGCGTTG TTGGCCAACA ATATTGGTCA GAAAATATTT 
GGCGAAGCCG TTTTGGGACT TTCGCAGGGA TCCGTATCTG AGCTGCTGAG CAAACCGAAG 
CCCTGGCACA TGCTCTCCAT CAAGGGCAGG GAACCCTTCA TCCGGATGCA ATTGTGGCTC 
AGTGATGCCA ACAATGTTGA ACGATTGCAG TTGCTGAAAA ATGAGCGACG GGAGGCCAGC 
AAACGAAGGA GGAGCACAGG CCCAAACCAA CAGGACAATA GTAGCGATAC CTCCAGCAAT 
GATACCAATG ACTTCTATAC CAGCAGTCCG GGTCCAGGAT CCGTCGGTTC GGGTGTCGGT 
GGTGCTCCGC CGAGCAAAAA ACAGCGTGTC CTCTTCTCCG AGGAGCAAAA GGAGGCACTT 
CGGCTGGCAT TCGCCTTGGA TCCGTATCCA AATGTGGGGA CCATCGAGTT TCTGGCCAAT 
GAGCTGGGCT TGGCAACGCG GACGATCACC AACTGGTTCC ACAATCATCG CATGCGATTG 
AAGCAACAGG TGCCACACGG ACCTGCCGGT CAAGATAATC CGATACCGAG TCGCGAGAGT 
ACCAGTGCCA CGCCCTTCGA TCCCGTCCAA TTCCGCATTC TGCTGCAGCA GCGTCTACTG 
GAGCTCCACA AGGAGCGAAT GGGCATGAGC GGTGCCCCCA TTCCCTATCC GCCGTACTTT 
GCTGCCGCTG CCATTTTGGG TCGCAGTCTG GCGGGGATTC CCGGTGCGGC AGCGGCCGCT 
GGAGCAGCTG CAGCAGCCGC CGCAGTTGGC GCCTCTGGTG GTGATGAGTT GCAGGCTCTG 
AATCAGGCCT TCAAGGAGCA GATGAGCGGT TTGGATCTGT CGATGCCCAC CTTAAAGCGG 
GAACGCAGCG ATGACTATCA GGACGATCTG GAACTGGAGG GTGGTGGTCA CAATCTAAGT 
GACAACGAAT CGCTGGAGGG TCAAGAGCCG GAGGACAAGA CCACCGATTA CGAGAAGGTG 
CTGCACAAAT CCGCTTTGGC CGCTGCCGCC GCTTACATGT CCAATGCAGT GCGGAGTTCA 
CGACGAAAGC CAGCTGCCCC ACAGTGGGTC AATCCCGCCG GAGCGGTGAC CAATCCAAGT 
GCTGTAGTTG CAGCCGTTGC TGCTGCCGCA GCTGCCGCGG CGGACAATGA ACGCATCATC 
AACGGGGTGT GCGTGATGCA GGCCTCCGAG TACGGCCGCG ATGATACTGA TAGCAACAAG 
CCAACGGACG GCGGCAACGA TTCCGATCAT GAGCACGCCC AGCTGGAGAT CGATCAGCGT 
TTCATGGAAC CGGAGGTGCA CATCAAACAG GAGGAGGACG ACGATGAGGA GCAATCGGGA 
TCGGTTAATC TGGACAACGA GGACAATGCG ACTAGCGAGC AAAAACTGAA GGTGATCAAC 
GAGGAGAAGC TGCGAATGGT GCGAGTTCGC CGGCTGAGCA GCACTGGTGG TGGTTCCTCT 
GAGGAAATGC CAGCTCCCCT CGCACCACCG CCTCCTCCGC CAGCAGCTTC TTCTTCTATC 
GTTAGTGGAG AAAGCACCAC CAGCAGCAGT AGTAGCAGCA ACACCAGCAG CAGCACACCG 
GCGGTAACCA CTGCAGCAGC AACTGCGGCA GCCGGTTGGA ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ct ( NM_001272381.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259310.1 Drosophila melanogaster cut (ct), transcript variant E, mRNA.

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CloneID	OFa03735
Clone ID Related Accession (Same CDS sequence)	NM_001272381.1 , NM_001272381.2
Accession Version	NM_001272381.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-15
Organism	Drosophila melanogaster(fruit fly)
Product	cut, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.18 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGCGCGCAA CCCTTGCCGA AAAGGAAGTG AACACACTCA AAGAGCAGCT ATCCACCGGC 
AATCCCGACA GCAACCTCAA CAGCGAAAAC AGCGATACAG CAGCAGCGGC AGCAACAGCA 
GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA ACAGCAACAA ACGACATCGA GGACGAGCAG 
CAGCAACAGT TGCAGCAGAC GGCCAGCGGC GGCATTTTGG AAAGCGACAG CGACAAGCTG 
CTGAACAGCT CCATTGTTGC AGCGGCCATA ACGCTGCAGC AGCAGAACGG CAGCAATCTG 
CTGGCCAACA CAAATACGCC GTCGCCCTCG CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG 
CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC GGTGTTGGCG GCGCCTGCCT CAATCCCAAG 
CTTTTCTTCA ATCACGCCCA ACAGATGATG ATGATGGAGG CGGCAGCCGC GGCAGCAGCG 
GCGGCATTGC AACAGCAGCA GCAACAGCAA TCACCACTCC ATTCGCCGGC GAATGAAGTT 
GCGATTCCCA CAGAACAGCC AGCGGCAACA GTGGCAACAG GAGCCGCTGC TGCAGCAGCC 
GCAGCAGCAA CACCGATTGC AACTGGCAAC GTCAAAAGCG GCAGCACCAC CAGCAACGCC 
AATCACACCA ACAGCAACAA TAGTCACCAG GACGAGGAGG AGCTGGACGA TGAGGAGGAG 
GACGAGGAGG AGGATGAGGA CGAGGATGAT GAGGAGGAGA ATGCCTCGAT GCAATCGAAT 
GCTGATGATA TGGAGCTGGA TGCGCAGCAA GAAACCAGAA CTGAGCCAAG TGCAACAACA 
CAACAGCAGC ATCAGCAGCA GGATACAGAG GATCTTGAGG AGAACAAGGA TGCGGGGGAG 
GCTAGTTTAA ATGTTAGCAA TAATCACAAT ACAACCGATA GCAATAATAG TTGTAGTCGA 
AAGAACAACA ATGGCGGCAA TGAAAGTGAG CAACATGTGG CCAGTTCGGC GGAGGACGAT 
GATTGCGCCA ACAACAATAC AAATACCAGC AATAACAACA ATACCAGCAA CACCGCCACC 
AGCAACACGA ACAACAACAA TAATAATAAC AGCAGCAGCG GCAACAGCGA GAAGCGCAAG 
AAGAAGAACA ATAACAACAA CAACGGCCAG CCTGCTGTTC TATTAGCTGC CAAAGATAAA 
GAGATTAAAG CCCTTTTGGA CGAACTGCAG CGCTTGAGGG CGCAGGAGCA GACGCACCTG 
GTGCAAATCC AACGATTGGA GGAACATCTT GAGGTCAAGC GTCAGCACAT CATCCGATTG 
GAGGCACGTC TGGATAAGCA GCAAATTAAT GAGGCACTGG CCGAGGCGAC CGCTTTGTCC 
GCTGCCGCGT CAACCAACAA CAACAATAAT AGCCAGAGCA GCGACAACAA TAAGAAGCTG 
AATACCGCTG CAGAAAGACC GATGGATGCG TCATCCAATG CCGATCTACC GGAGTCCACA 
AAAGCTCCAG TTCCGGCGGA GGATGACGAA GAAGATGAGG ATCAGGCCAT GCTGGTGGAT 
TCCGAGGAAG CGGAGGACAA GCCAGAGGAC TCACATCACG ATGACGACGA AGATGAGGAC 
GAGGATCGGG AGGCGGTCAA TGCCACCACA ACCGATAGCA ACGAGCTCAA AATCAAAAAG 
GAACAGCATC CGCTGGATCT GAATGTGCTC AGTCCGAATT CGGCCATTGC CGCTGCAGCT 
GCGGCCGCCG CTGCCGCCGC CTGCGCCAAT GACCCCAACA AATTCCAGGC TCTGCTCATC 
GAGCGGACCA AAGCCTTGGC CGCAGAGGCG CTCAAGAATG GCGCCAGCGA TGCCCTCAGC 
GAAGATGCCC ACCATCAGCA GCAGCAGCAC CACCAACAGC AGCACCAACA CCAGCAACAA 
CACCACCAGC AACAGCACCT CCACCAGCAA CATCACCATC ACCTGCAACA GCAACCAAAC 
AGCGGCAGCA ACAGCAATCC AGCGAGCAAC GACCACCATC ATGGACACCA TTTGCATGGC 
CATGGTTTGC TGCATCCTTC GTCGGCGCAC CATCTGCACC ACCAAACGAC CGAATCCAAT 
TCGAATTCGA GTACACCCAC AGCGGCGGGA AATAACAATG GTAGCAATAA CAGCTCCAGC 
AACACAAATG CCAATAGTAC CGCTCAATTG GCCGCCAGTT TGGCCAGTAC ACTCAATGGC 
ACCAAGTCCC TGATGCAGGA GGATAGCAAT GGCTTGGCCG CCGTCGCCAT GGCGGCTCAT 
GCCCAACATG CGGCTGCCTT GGGACCTGGA TTTTTGCCCG GCCTTCCAGC GTTTCAGTTT 
GCCGCCGCCC AAGTGGCCGC CGGTGGCGAT GGACGGGGTC ATTATCGTTT CGCCGACTCG 
GAACTGCAAT TGCCACCGGG TGCTTCGATG GCCGGTCGTC TTGGAGAGTC GCTCATACCC 
AAGGGTGATC CCATGGAGGC CAAACTCCAA GAGATGTTGC GCTACAACAT GGACAAGTAC 
GCCAATCAGG CGCTGGATAC CCTGCACATT TCGCGACGTG TCCGCGAACT GCTATCGGTG 
CACAACATTG GCCAGCGACT ATTTGCCAAA TACATACTGG GCCTGTCGCA GGGCACCGTT 
TCCGAGCTGC TGAGCAAACC CAAGCCGTGG GACAAGTTGA CGGAGAAGGG ACGAGATAGC 
TACCGCAAAA TGCACGCCTG GGCCTGTGAC GATAATGCCG TCATGCTGCT CAAATCGCTG 
ATACCCAAGA AAGATTCTGG ACTGCCGCAG TATGCGGGTC GTGGTGCCGG CGGCGCTGGC 
GGCGATGATT CCATGTCCGA GGATCGGATT GCACATATCC TCAGCGAAGC CTCCTCGCTG 
ATGAAACAGA GCAGTGTGGC CCAGCATCGG GAGCAGGAGC GTCGCAGTCA CGGCGGCGAG 
GATTCACACA GCAACGAGGA CAGCAAATCG CCACCTCAAA GCTGCACGTC GCCGTTCTTC 
AAGGTGGAGA ATCAGCTGAA GCAGCACCAG CATCTTAATC CCGAGCAGGC TGCCGCTCAG 
CAAAGGGAAA GGGAACGCGA GCAAAGAGAA CGGGAGCAGC AGCAGCGGCT GCGGCACGAC 
GACCAGGACA AGATGGCTCG TCTCTACCAG GAGCTGATTG CACGCACTCC GCGTGAGACG 
GCTTTTCCAA GCTTTCTCTT CTCACCCTCG CTATTTGGAG GAGCCGCCGG AATGCCAGGA 
GCAGCCAGCA ATGCCTTTCC CGCCATGGCC GATGAAAATA TGCGTCACGT CTTTGAACGT 
GAGATTGCCA AGCTGCAGCA GCACCAACAG CAACAACAAG CTGCCCAGGC TCAGGCACAG 
TTCCCCAACT TCTCGAGCCT GATGGCTTTG CAGCAGCAGG TCCTCAACGG AGCCCAGGAT 
CTCTCGCTAG CTGCCGCTGC AGCCAAGGAC ATCAAGTTGA ACGGTCAGCG ATCCTCGTTG 
GAGCACAGTG CCGGCAGCAG CAGTTGTTCA AAAGATGGCG AACGAGATGA TGCGTATCCA 
AGTTCCCTAC ATGGTCGCAA GTCCGAGGGA GGTGGCACAC CCGCACCACC TGCCCCACCA 
TCTGGCCCAG GAACCGGAGC AGGAGCCCCA CCCACAGCAG CACCACCAAC CGGCGGTGCC 
AGCAGCAATT CGGCAGCACC CAGTCCGCTA AGCAATTCAA TACTTCCACC GGCATTGAGC 
AGCCAAGGTG AGGAGTTCGC CGCGACAGCA AGTCCTTTGC AGCGAATGGC CTCGATCACC 
AATTCGCTGA TTACCCAACC ACCGGTGACG CCGCACCACA GTACGCCACA GCGACCCACC 
AAGGCGGTTC TGCCACCGAT CACCCAGCAA CAGTTCGATA TGTTCAACAA CCTTAACACC 
GAGGATATAG TCAGACGTGT GAAGGAGGCC CTGTCGCAGT ACTCCATCTC CCAGCGATTG 
TTCGGCGAGT CCGTGCTGGG TTTGTCTCAG GGATCGGTCT CCGATTTATT GGCTAGACCC 
AAGCCATGGC ACATGCTCAC TCAAAAGGGC CGTGAACCCT TCATTCGCAT GAAGATGTTC 
CTGGAGGATG AGAACGCGGT GCACAAACTG GTGGCCAGTC AATACAAGAT TGCCCCCGAG 
AAGCTAATGC GAACGGGCAG TTACAGTGGA AGTCCGCAGA TGCCACAGGG ATTGGCAAGC 
AAAATGCAGG CTGCCTCCCT GCCCATGCAG AAGATGATGA GCGAACTTAA GCTGCAGGAG 
CCAGCACAGG CGCAGCACTT GATGCAGCAA ATGCAGGCGG CGGCAATGTC GGCAGCAATG 
CAACAGCAGC AAGTGGCACA AGCGCAGCAG CAGGCTCAGC AGGCGCAACA GGCACAACAG 
CATTTGCAGC AGCAGGCGCA GCAGCACTTG CAACAGCAGC AACATCTTGC CCAGCAGCAA 
CACCCCCATC AGCAGCACCA TCAAGCGGCC GCCGCGGCTG CCGCATTGCA CCACCAAAGC 
ATGCTTCTGA CCTCGCCCGG ACTGCCACCC CAGCATGCCA TCAGTCTGCC ACCTTCAGCG 
GGTGGAGCCC AACCAGGTGG TCCTGGTGGC AATCAGGGCA GCTCAAATCC CTCAAACAGC 
GAAAAGAAAC CCATGCTGAT GCCGGTTCAT GGCACTAATG CCATGAGAAG TCTGCACCAG 
CACATGTCGC CCACCGTTTA CGAAATGGCC GCTTTAACCC AAGATCTGGA CACCCACGAT 
ATCACTACCA AGATCAAGGA GGCGTTGTTG GCCAACAATA TTGGTCAGAA AATATTTGGC 
GAAGCCGTTT TGGGACTTTC GCAGGGATCC GTATCTGAGC TGCTGAGCAA ACCGAAGCCC 
TGGCACATGC TCTCCATCAA GGGCAGGGAA CCCTTCATCC GGATGCAATT GTGGCTCAGT 
GATGCCAACA ATGTTGAACG ATTGCAGTTG CTGAAAAATG AGCGACGGGA GGCCAGCAAA 
CGAAGGAGGA GCACAGGCCC AAACCAACAG GACAATAGTA GCGATACCTC CAGCAATGAT 
ACCAATGACT TCTATACCAG CAGTCCGGGT CCAGGATCCG TCGGTTCGGG TGTCGGTGGT 
GCTCCGCCGA GCAAAAAACA GCGTGTCCTC TTCTCCGAGG AGCAAAAGGA GGCACTTCGG 
CTGGCATTCG CCTTGGATCC GTATCCAAAT GTGGGGACCA TCGAGTTTCT GGCCAATGAG 
CTGGGCTTGG CAACGCGGAC GATCACCAAC TGGTTCCACA ATCATCGCAT GCGATTGAAG 
CAACAGGTGC CACACGGACC TGCCGGTCAA GATAATCCGA TACCGAGTCG CGAGAGTACC 
AGTGCCACGC CCTTCGATCC CGTCCAATTC CGCATTCTGC TGCAGCAGCG TCTACTGGAG 
CTCCACAAGG AGCGAATGGG CATGAGCGGT GCCCCCATTC CCTATCCGCC GTACTTTGCT 
GCCGCTGCCA TTTTGGGTCG CAGTCTGGCG GGGATTCCCG GTGCGGCAGC GGCCGCTGGA 
GCAGCTGCAG CAGCCGCCGC AGTTGGCGCC TCTGGTGGTG ATGAGTTGCA GGCTCTGAAT 
CAGGCCTTCA AGGAGCAGAT GAGCGGTTTG GATCTGTCGA TGCCCACCTT AAAGCGGGAA 
CGCAGCGATG ACTATCAGGA CGATCTGGAA CTGGAGGGTG GTGGTCACAA TCTAAGTGAC 
AACGAATCGC TGGAGGGTCA AGAGCCGGAG GACAAGACCA CCGATTACGA GAAGGTGCTG 
CACAAATCCG CTTTGGCCGC TGCCGCCGCT TACATGTCCA ATGCAGTGCG GAGTTCACGA 
CGAAAGCCAG CTGCCCCACA GTGGGTCAAT CCCGCCGGAG CGGTGACCAA TCCAAGTGCT 
GTAGTTGCAG CCGTTGCTGC TGCCGCAGCT GCCGCGGCGG ACAATGAACG CATCATCAAC 
GGGGTGTGCG TGATGCAGGC CTCCGAGTAC GGCCGCGATG ATACTGATAG CAACAAGCCA 
ACGGACGGCG GCAACGATTC CGATCATGAG CACGCCCAGC TGGAGATCGA TCAGCGTTTC 
ATGGAACCGG AGGTGCACAT CAAACAGGAG GAGGACGACG ATGAGGAGCA ATCGGGATCG 
GTTAATCTGG ACAACGAGGA CAATGCGACT AGCGAGCAAA AACTGAAGGT GATCAACGAG 
GAGAAGCTGC GAATGGTGCG AGTTCGCCGG CTGAGCAGCA CTGGTGGTGG TTCCTCTGAG 
GAAATGCCAG CTCCCCTCGC ACCACCGCCT CCTCCGCCAG CAGCTTCTTC TTCTATCGTT 
AGTGGAGAAA GCACCACCAG CAGCAGTAGT AGCAGCAACA CCAGCAGCAG CACACCGGCG 
GTAACCACTG CAGCAGCAAC TGCGGCAGCC GGTTGGAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259310.1
CDS	269..6793
Translation	MQPTLPQAAGTADMDLTAVQSINDWFFKKEQIYLLAQFWQQRATLAEKEVNTLKEQLSTGNPDSNLNSENSDTAAAAATAAAVAAVVAGATATNDIEDEQQQQLQQTASGGILESDSDKLLNSSIVAAAITLQQQNGSNLLANTNTPSPSPPLLSAEQQQQLQSSLQQSGGVGGACLNPKLFFNHAQQMMMMEAAAAAAAAALQQQQQQQSPLHSPANEVAIPTEQPAATVATGAAAAAAAAATPIATGNVKSGSTTSNANHTNSNNSHQDEEELDDEEEDEEEDEDEDDEEENASMQSNADDMELDAQQETRTEPSATTQQQHQQQDTEDLEENKDAGEASLNVSNNHNTTDSNNSCSRKNNNGGNESEQHVASSAEDDDCANNNTNTSNNNNTSNTATSNTNNNNNNNSSSGNSEKRKKKNNNNNNGQPAVLLAAKDKEIKALLDELQRLRAQEQTHLVQIQRLEEHLEVKRQHIIRLEARLDKQQINEALAEATALSAAASTNNNNNSQSSDNNKKLNTAAERPMDASSNADLPESTKAPVPAEDDEEDEDQAMLVDSEEAEDKPEDSHHDDDEDEDEDREAVNATTTDSNELKIKKEQHPLDLNVLSPNSAIAAAAAAAAAAACANDPNKFQALLIERTKALAAEALKNGASDALSEDAHHQQQQHHQQQHQHQQQHHQQQHLHQQHHHHLQQQPNSGSNSNPASNDHHHGHHLHGHGLLHPSSAHHLHHQTTESNSNSSTPTAAGNNNGSNNSSSNTNANSTAQLAASLASTLNGTKSLMQEDSNGLAAVAMAAHAQHAAALGPGFLPGLPAFQFAAAQVAAGGDGRGHYRFADSELQLPPGASMAGRLGESLIPKGDPMEAKLQEMLRYNMDKYANQALDTLHISRRVRELLSVHNIGQRLFAKYILGLSQGTVSELLSKPKPWDKLTEKGRDSYRKMHAWACDDNAVMLLKSLIPKKDSGLPQYAGRGAGGAGGDDSMSEDRIAHILSEASSLMKQSSVAQHREQERRSHGGEDSHSNEDSKSPPQSCTSPFFKVENQLKQHQHLNPEQAAAQQREREREQREREQQQRLRHDDQDKMARLYQELIARTPRETAFPSFLFSPSLFGGAAGMPGAASNAFPAMADENMRHVFEREIAKLQQHQQQQQAAQAQAQFPNFSSLMALQQQVLNGAQDLSLAAAAAKDIKLNGQRSSLEHSAGSSSCSKDGERDDAYPSSLHGRKSEGGGTPAPPAPPSGPGTGAGAPPTAAPPTGGASSNSAAPSPLSNSILPPALSSQGEEFAATASPLQRMASITNSLITQPPVTPHHSTPQRPTKAVLPPITQQQFDMFNNLNTEDIVRRVKEALSQYSISQRLFGESVLGLSQGSVSDLLARPKPWHMLTQKGREPFIRMKMFLEDENAVHKLVASQYKIAPEKLMRTGSYSGSPQMPQGLASKMQAASLPMQKMMSELKLQEPAQAQHLMQQMQAAAMSAAMQQQQVAQAQQQAQQAQQAQQHLQQQAQQHLQQQQHLAQQQHPHQQHHQAAAAAAALHHQSMLLTSPGLPPQHAISLPPSAGGAQPGGPGGNQGSSNPSNSEKKPMLMPVHGTNAMRSLHQHMSPTVYEMAALTQDLDTHDITTKIKEALLANNIGQKIFGEAVLGLSQGSVSELLSKPKPWHMLSIKGREPFIRMQLWLSDANNVERLQLLKNERREASKRRRSTGPNQQDNSSDTSSNDTNDFYTSSPGPGSVGSGVGGAPPSKKQRVLFSEEQKEALRLAFALDPYPNVGTIEFLANELGLATRTITNWFHNHRMRLKQQVPHGPAGQDNPIPSRESTSATPFDPVQFRILLQQRLLELHKERMGMSGAPIPYPPYFAAAAILGRSLAGIPGAAAAAGAAAAAAAVGASGGDELQALNQAFKEQMSGLDLSMPTLKRERSDDYQDDLELEGGGHNLSDNESLEGQEPEDKTTDYEKVLHKSALAAAAAYMSNAVRSSRRKPAAPQWVNPAGAVTNPSAVVAAVAAAAAAAADNERIINGVCVMQASEYGRDDTDSNKPTDGGNDSDHEHAQLEIDQRFMEPEVHIKQEEDDDEEQSGSVNLDNEDNATSEQKLKVINEEKLRMVRVRRLSSTGGGSSEEMPAPLAPPPPPPAASSSIVSGESTTSSSSSSNTSSSTPAVTTAAATAAAGWNY

Target ORF information:

RefSeq Version	NM_001272381.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cut (ct), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272381.1

ORF Insert Sequence:

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGCGCGCAA CCCTTGCCGA AAAGGAAGTG AACACACTCA AAGAGCAGCT ATCCACCGGC 
AATCCCGACA GCAACCTCAA CAGCGAAAAC AGCGATACAG CAGCAGCGGC AGCAACAGCA 
GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA ACAGCAACAA ACGACATCGA GGACGAGCAG 
CAGCAACAGT TGCAGCAGAC GGCCAGCGGC GGCATTTTGG AAAGCGACAG CGACAAGCTG 
CTGAACAGCT CCATTGTTGC AGCGGCCATA ACGCTGCAGC AGCAGAACGG CAGCAATCTG 
CTGGCCAACA CAAATACGCC GTCGCCCTCG CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG 
CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC GGTGTTGGCG GCGCCTGCCT CAATCCCAAG 
CTTTTCTTCA ATCACGCCCA ACAGATGATG ATGATGGAGG CGGCAGCCGC GGCAGCAGCG 
GCGGCATTGC AACAGCAGCA GCAACAGCAA TCACCACTCC ATTCGCCGGC GAATGAAGTT 
GCGATTCCCA CAGAACAGCC AGCGGCAACA GTGGCAACAG GAGCCGCTGC TGCAGCAGCC 
GCAGCAGCAA CACCGATTGC AACTGGCAAC GTCAAAAGCG GCAGCACCAC CAGCAACGCC 
AATCACACCA ACAGCAACAA TAGTCACCAG GACGAGGAGG AGCTGGACGA TGAGGAGGAG 
GACGAGGAGG AGGATGAGGA CGAGGATGAT GAGGAGGAGA ATGCCTCGAT GCAATCGAAT 
GCTGATGATA TGGAGCTGGA TGCGCAGCAA GAAACCAGAA CTGAGCCAAG TGCAACAACA 
CAACAGCAGC ATCAGCAGCA GGATACAGAG GATCTTGAGG AGAACAAGGA TGCGGGGGAG 
GCTAGTTTAA ATGTTAGCAA TAATCACAAT ACAACCGATA GCAATAATAG TTGTAGTCGA 
AAGAACAACA ATGGCGGCAA TGAAAGTGAG CAACATGTGG CCAGTTCGGC GGAGGACGAT 
GATTGCGCCA ACAACAATAC AAATACCAGC AATAACAACA ATACCAGCAA CACCGCCACC 
AGCAACACGA ACAACAACAA TAATAATAAC AGCAGCAGCG GCAACAGCGA GAAGCGCAAG 
AAGAAGAACA ATAACAACAA CAACGGCCAG CCTGCTGTTC TATTAGCTGC CAAAGATAAA 
GAGATTAAAG CCCTTTTGGA CGAACTGCAG CGCTTGAGGG CGCAGGAGCA GACGCACCTG 
GTGCAAATCC AACGATTGGA GGAACATCTT GAGGTCAAGC GTCAGCACAT CATCCGATTG 
GAGGCACGTC TGGATAAGCA GCAAATTAAT GAGGCACTGG CCGAGGCGAC CGCTTTGTCC 
GCTGCCGCGT CAACCAACAA CAACAATAAT AGCCAGAGCA GCGACAACAA TAAGAAGCTG 
AATACCGCTG CAGAAAGACC GATGGATGCG TCATCCAATG CCGATCTACC GGAGTCCACA 
AAAGCTCCAG TTCCGGCGGA GGATGACGAA GAAGATGAGG ATCAGGCCAT GCTGGTGGAT 
TCCGAGGAAG CGGAGGACAA GCCAGAGGAC TCACATCACG ATGACGACGA AGATGAGGAC 
GAGGATCGGG AGGCGGTCAA TGCCACCACA ACCGATAGCA ACGAGCTCAA AATCAAAAAG 
GAACAGCATC CGCTGGATCT GAATGTGCTC AGTCCGAATT CGGCCATTGC CGCTGCAGCT 
GCGGCCGCCG CTGCCGCCGC CTGCGCCAAT GACCCCAACA AATTCCAGGC TCTGCTCATC 
GAGCGGACCA AAGCCTTGGC CGCAGAGGCG CTCAAGAATG GCGCCAGCGA TGCCCTCAGC 
GAAGATGCCC ACCATCAGCA GCAGCAGCAC CACCAACAGC AGCACCAACA CCAGCAACAA 
CACCACCAGC AACAGCACCT CCACCAGCAA CATCACCATC ACCTGCAACA GCAACCAAAC 
AGCGGCAGCA ACAGCAATCC AGCGAGCAAC GACCACCATC ATGGACACCA TTTGCATGGC 
CATGGTTTGC TGCATCCTTC GTCGGCGCAC CATCTGCACC ACCAAACGAC CGAATCCAAT 
TCGAATTCGA GTACACCCAC AGCGGCGGGA AATAACAATG GTAGCAATAA CAGCTCCAGC 
AACACAAATG CCAATAGTAC CGCTCAATTG GCCGCCAGTT TGGCCAGTAC ACTCAATGGC 
ACCAAGTCCC TGATGCAGGA GGATAGCAAT GGCTTGGCCG CCGTCGCCAT GGCGGCTCAT 
GCCCAACATG CGGCTGCCTT GGGACCTGGA TTTTTGCCCG GCCTTCCAGC GTTTCAGTTT 
GCCGCCGCCC AAGTGGCCGC CGGTGGCGAT GGACGGGGTC ATTATCGTTT CGCCGACTCG 
GAACTGCAAT TGCCACCGGG TGCTTCGATG GCCGGTCGTC TTGGAGAGTC GCTCATACCC 
AAGGGTGATC CCATGGAGGC CAAACTCCAA GAGATGTTGC GCTACAACAT GGACAAGTAC 
GCCAATCAGG CGCTGGATAC CCTGCACATT TCGCGACGTG TCCGCGAACT GCTATCGGTG 
CACAACATTG GCCAGCGACT ATTTGCCAAA TACATACTGG GCCTGTCGCA GGGCACCGTT 
TCCGAGCTGC TGAGCAAACC CAAGCCGTGG GACAAGTTGA CGGAGAAGGG ACGAGATAGC 
TACCGCAAAA TGCACGCCTG GGCCTGTGAC GATAATGCCG TCATGCTGCT CAAATCGCTG 
ATACCCAAGA AAGATTCTGG ACTGCCGCAG TATGCGGGTC GTGGTGCCGG CGGCGCTGGC 
GGCGATGATT CCATGTCCGA GGATCGGATT GCACATATCC TCAGCGAAGC CTCCTCGCTG 
ATGAAACAGA GCAGTGTGGC CCAGCATCGG GAGCAGGAGC GTCGCAGTCA CGGCGGCGAG 
GATTCACACA GCAACGAGGA CAGCAAATCG CCACCTCAAA GCTGCACGTC GCCGTTCTTC 
AAGGTGGAGA ATCAGCTGAA GCAGCACCAG CATCTTAATC CCGAGCAGGC TGCCGCTCAG 
CAAAGGGAAA GGGAACGCGA GCAAAGAGAA CGGGAGCAGC AGCAGCGGCT GCGGCACGAC 
GACCAGGACA AGATGGCTCG TCTCTACCAG GAGCTGATTG CACGCACTCC GCGTGAGACG 
GCTTTTCCAA GCTTTCTCTT CTCACCCTCG CTATTTGGAG GAGCCGCCGG AATGCCAGGA 
GCAGCCAGCA ATGCCTTTCC CGCCATGGCC GATGAAAATA TGCGTCACGT CTTTGAACGT 
GAGATTGCCA AGCTGCAGCA GCACCAACAG CAACAACAAG CTGCCCAGGC TCAGGCACAG 
TTCCCCAACT TCTCGAGCCT GATGGCTTTG CAGCAGCAGG TCCTCAACGG AGCCCAGGAT 
CTCTCGCTAG CTGCCGCTGC AGCCAAGGAC ATCAAGTTGA ACGGTCAGCG ATCCTCGTTG 
GAGCACAGTG CCGGCAGCAG CAGTTGTTCA AAAGATGGCG AACGAGATGA TGCGTATCCA 
AGTTCCCTAC ATGGTCGCAA GTCCGAGGGA GGTGGCACAC CCGCACCACC TGCCCCACCA 
TCTGGCCCAG GAACCGGAGC AGGAGCCCCA CCCACAGCAG CACCACCAAC CGGCGGTGCC 
AGCAGCAATT CGGCAGCACC CAGTCCGCTA AGCAATTCAA TACTTCCACC GGCATTGAGC 
AGCCAAGGTG AGGAGTTCGC CGCGACAGCA AGTCCTTTGC AGCGAATGGC CTCGATCACC 
AATTCGCTGA TTACCCAACC ACCGGTGACG CCGCACCACA GTACGCCACA GCGACCCACC 
AAGGCGGTTC TGCCACCGAT CACCCAGCAA CAGTTCGATA TGTTCAACAA CCTTAACACC 
GAGGATATAG TCAGACGTGT GAAGGAGGCC CTGTCGCAGT ACTCCATCTC CCAGCGATTG 
TTCGGCGAGT CCGTGCTGGG TTTGTCTCAG GGATCGGTCT CCGATTTATT GGCTAGACCC 
AAGCCATGGC ACATGCTCAC TCAAAAGGGC CGTGAACCCT TCATTCGCAT GAAGATGTTC 
CTGGAGGATG AGAACGCGGT GCACAAACTG GTGGCCAGTC AATACAAGAT TGCCCCCGAG 
AAGCTAATGC GAACGGGCAG TTACAGTGGA AGTCCGCAGA TGCCACAGGG ATTGGCAAGC 
AAAATGCAGG CTGCCTCCCT GCCCATGCAG AAGATGATGA GCGAACTTAA GCTGCAGGAG 
CCAGCACAGG CGCAGCACTT GATGCAGCAA ATGCAGGCGG CGGCAATGTC GGCAGCAATG 
CAACAGCAGC AAGTGGCACA AGCGCAGCAG CAGGCTCAGC AGGCGCAACA GGCACAACAG 
CATTTGCAGC AGCAGGCGCA GCAGCACTTG CAACAGCAGC AACATCTTGC CCAGCAGCAA 
CACCCCCATC AGCAGCACCA TCAAGCGGCC GCCGCGGCTG CCGCATTGCA CCACCAAAGC 
ATGCTTCTGA CCTCGCCCGG ACTGCCACCC CAGCATGCCA TCAGTCTGCC ACCTTCAGCG 
GGTGGAGCCC AACCAGGTGG TCCTGGTGGC AATCAGGGCA GCTCAAATCC CTCAAACAGC 
GAAAAGAAAC CCATGCTGAT GCCGGTTCAT GGCACTAATG CCATGAGAAG TCTGCACCAG 
CACATGTCGC CCACCGTTTA CGAAATGGCC GCTTTAACCC AAGATCTGGA CACCCACGAT 
ATCACTACCA AGATCAAGGA GGCGTTGTTG GCCAACAATA TTGGTCAGAA AATATTTGGC 
GAAGCCGTTT TGGGACTTTC GCAGGGATCC GTATCTGAGC TGCTGAGCAA ACCGAAGCCC 
TGGCACATGC TCTCCATCAA GGGCAGGGAA CCCTTCATCC GGATGCAATT GTGGCTCAGT 
GATGCCAACA ATGTTGAACG ATTGCAGTTG CTGAAAAATG AGCGACGGGA GGCCAGCAAA 
CGAAGGAGGA GCACAGGCCC AAACCAACAG GACAATAGTA GCGATACCTC CAGCAATGAT 
ACCAATGACT TCTATACCAG CAGTCCGGGT CCAGGATCCG TCGGTTCGGG TGTCGGTGGT 
GCTCCGCCGA GCAAAAAACA GCGTGTCCTC TTCTCCGAGG AGCAAAAGGA GGCACTTCGG 
CTGGCATTCG CCTTGGATCC GTATCCAAAT GTGGGGACCA TCGAGTTTCT GGCCAATGAG 
CTGGGCTTGG CAACGCGGAC GATCACCAAC TGGTTCCACA ATCATCGCAT GCGATTGAAG 
CAACAGGTGC CACACGGACC TGCCGGTCAA GATAATCCGA TACCGAGTCG CGAGAGTACC 
AGTGCCACGC CCTTCGATCC CGTCCAATTC CGCATTCTGC TGCAGCAGCG TCTACTGGAG 
CTCCACAAGG AGCGAATGGG CATGAGCGGT GCCCCCATTC CCTATCCGCC GTACTTTGCT 
GCCGCTGCCA TTTTGGGTCG CAGTCTGGCG GGGATTCCCG GTGCGGCAGC GGCCGCTGGA 
GCAGCTGCAG CAGCCGCCGC AGTTGGCGCC TCTGGTGGTG ATGAGTTGCA GGCTCTGAAT 
CAGGCCTTCA AGGAGCAGAT GAGCGGTTTG GATCTGTCGA TGCCCACCTT AAAGCGGGAA 
CGCAGCGATG ACTATCAGGA CGATCTGGAA CTGGAGGGTG GTGGTCACAA TCTAAGTGAC 
AACGAATCGC TGGAGGGTCA AGAGCCGGAG GACAAGACCA CCGATTACGA GAAGGTGCTG 
CACAAATCCG CTTTGGCCGC TGCCGCCGCT TACATGTCCA ATGCAGTGCG GAGTTCACGA 
CGAAAGCCAG CTGCCCCACA GTGGGTCAAT CCCGCCGGAG CGGTGACCAA TCCAAGTGCT 
GTAGTTGCAG CCGTTGCTGC TGCCGCAGCT GCCGCGGCGG ACAATGAACG CATCATCAAC 
GGGGTGTGCG TGATGCAGGC CTCCGAGTAC GGCCGCGATG ATACTGATAG CAACAAGCCA 
ACGGACGGCG GCAACGATTC CGATCATGAG CACGCCCAGC TGGAGATCGA TCAGCGTTTC 
ATGGAACCGG AGGTGCACAT CAAACAGGAG GAGGACGACG ATGAGGAGCA ATCGGGATCG 
GTTAATCTGG ACAACGAGGA CAATGCGACT AGCGAGCAAA AACTGAAGGT GATCAACGAG 
GAGAAGCTGC GAATGGTGCG AGTTCGCCGG CTGAGCAGCA CTGGTGGTGG TTCCTCTGAG 
GAAATGCCAG CTCCCCTCGC ACCACCGCCT CCTCCGCCAG CAGCTTCTTC TTCTATCGTT 
AGTGGAGAAA GCACCACCAG CAGCAGTAGT AGCAGCAACA CCAGCAGCAG CACACCGGCG 
GTAACCACTG CAGCAGCAAC TGCGGCAGCC GGTTGGAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ct ( NM_001272381.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259310.1 Drosophila melanogaster cut (ct), transcript variant E, mRNA.

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CloneID	OFa03735
Clone ID Related Accession (Same CDS sequence)	NM_001272381.1 , NM_001272381.2
Accession Version	NM_001272381.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cut, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Apr 25, 2019 this sequence version replaced NM_001272381.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGCGCGCAA CCCTTGCCGA AAAGGAAGTG AACACACTCA AAGAGCAGCT ATCCACCGGC 
AATCCCGACA GCAACCTCAA CAGCGAAAAC AGCGATACAG CAGCAGCGGC AGCAACAGCA 
GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA ACAGCAACAA ACGACATCGA GGACGAGCAG 
CAGCAACAGT TGCAGCAGAC GGCCAGCGGC GGCATTTTGG AAAGCGACAG CGACAAGCTG 
CTGAACAGCT CCATTGTTGC AGCGGCCATA ACGCTGCAGC AGCAGAACGG CAGCAATCTG 
CTGGCCAACA CAAATACGCC GTCGCCCTCG CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG 
CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC GGTGTTGGCG GCGCCTGCCT CAATCCCAAG 
CTTTTCTTCA ATCACGCCCA ACAGATGATG ATGATGGAGG CGGCAGCCGC GGCAGCAGCG 
GCGGCATTGC AACAGCAGCA GCAACAGCAA TCACCACTCC ATTCGCCGGC GAATGAAGTT 
GCGATTCCCA CAGAACAGCC AGCGGCAACA GTGGCAACAG GAGCCGCTGC TGCAGCAGCC 
GCAGCAGCAA CACCGATTGC AACTGGCAAC GTCAAAAGCG GCAGCACCAC CAGCAACGCC 
AATCACACCA ACAGCAACAA TAGTCACCAG GACGAGGAGG AGCTGGACGA TGAGGAGGAG 
GACGAGGAGG AGGATGAGGA CGAGGATGAT GAGGAGGAGA ATGCCTCGAT GCAATCGAAT 
GCTGATGATA TGGAGCTGGA TGCGCAGCAA GAAACCAGAA CTGAGCCAAG TGCAACAACA 
CAACAGCAGC ATCAGCAGCA GGATACAGAG GATCTTGAGG AGAACAAGGA TGCGGGGGAG 
GCTAGTTTAA ATGTTAGCAA TAATCACAAT ACAACCGATA GCAATAATAG TTGTAGTCGA 
AAGAACAACA ATGGCGGCAA TGAAAGTGAG CAACATGTGG CCAGTTCGGC GGAGGACGAT 
GATTGCGCCA ACAACAATAC AAATACCAGC AATAACAACA ATACCAGCAA CACCGCCACC 
AGCAACACGA ACAACAACAA TAATAATAAC AGCAGCAGCG GCAACAGCGA GAAGCGCAAG 
AAGAAGAACA ATAACAACAA CAACGGCCAG CCTGCTGTTC TATTAGCTGC CAAAGATAAA 
GAGATTAAAG CCCTTTTGGA CGAACTGCAG CGCTTGAGGG CGCAGGAGCA GACGCACCTG 
GTGCAAATCC AACGATTGGA GGAACATCTT GAGGTCAAGC GTCAGCACAT CATCCGATTG 
GAGGCACGTC TGGATAAGCA GCAAATTAAT GAGGCACTGG CCGAGGCGAC CGCTTTGTCC 
GCTGCCGCGT CAACCAACAA CAACAATAAT AGCCAGAGCA GCGACAACAA TAAGAAGCTG 
AATACCGCTG CAGAAAGACC GATGGATGCG TCATCCAATG CCGATCTACC GGAGTCCACA 
AAAGCTCCAG TTCCGGCGGA GGATGACGAA GAAGATGAGG ATCAGGCCAT GCTGGTGGAT 
TCCGAGGAAG CGGAGGACAA GCCAGAGGAC TCACATCACG ATGACGACGA AGATGAGGAC 
GAGGATCGGG AGGCGGTCAA TGCCACCACA ACCGATAGCA ACGAGCTCAA AATCAAAAAG 
GAACAGCATC CGCTGGATCT GAATGTGCTC AGTCCGAATT CGGCCATTGC CGCTGCAGCT 
GCGGCCGCCG CTGCCGCCGC CTGCGCCAAT GACCCCAACA AATTCCAGGC TCTGCTCATC 
GAGCGGACCA AAGCCTTGGC CGCAGAGGCG CTCAAGAATG GCGCCAGCGA TGCCCTCAGC 
GAAGATGCCC ACCATCAGCA GCAGCAGCAC CACCAACAGC AGCACCAACA CCAGCAACAA 
CACCACCAGC AACAGCACCT CCACCAGCAA CATCACCATC ACCTGCAACA GCAACCAAAC 
AGCGGCAGCA ACAGCAATCC AGCGAGCAAC GACCACCATC ATGGACACCA TTTGCATGGC 
CATGGTTTGC TGCATCCTTC GTCGGCGCAC CATCTGCACC ACCAAACGAC CGAATCCAAT 
TCGAATTCGA GTACACCCAC AGCGGCGGGA AATAACAATG GTAGCAATAA CAGCTCCAGC 
AACACAAATG CCAATAGTAC CGCTCAATTG GCCGCCAGTT TGGCCAGTAC ACTCAATGGC 
ACCAAGTCCC TGATGCAGGA GGATAGCAAT GGCTTGGCCG CCGTCGCCAT GGCGGCTCAT 
GCCCAACATG CGGCTGCCTT GGGACCTGGA TTTTTGCCCG GCCTTCCAGC GTTTCAGTTT 
GCCGCCGCCC AAGTGGCCGC CGGTGGCGAT GGACGGGGTC ATTATCGTTT CGCCGACTCG 
GAACTGCAAT TGCCACCGGG TGCTTCGATG GCCGGTCGTC TTGGAGAGTC GCTCATACCC 
AAGGGTGATC CCATGGAGGC CAAACTCCAA GAGATGTTGC GCTACAACAT GGACAAGTAC 
GCCAATCAGG CGCTGGATAC CCTGCACATT TCGCGACGTG TCCGCGAACT GCTATCGGTG 
CACAACATTG GCCAGCGACT ATTTGCCAAA TACATACTGG GCCTGTCGCA GGGCACCGTT 
TCCGAGCTGC TGAGCAAACC CAAGCCGTGG GACAAGTTGA CGGAGAAGGG ACGAGATAGC 
TACCGCAAAA TGCACGCCTG GGCCTGTGAC GATAATGCCG TCATGCTGCT CAAATCGCTG 
ATACCCAAGA AAGATTCTGG ACTGCCGCAG TATGCGGGTC GTGGTGCCGG CGGCGCTGGC 
GGCGATGATT CCATGTCCGA GGATCGGATT GCACATATCC TCAGCGAAGC CTCCTCGCTG 
ATGAAACAGA GCAGTGTGGC CCAGCATCGG GAGCAGGAGC GTCGCAGTCA CGGCGGCGAG 
GATTCACACA GCAACGAGGA CAGCAAATCG CCACCTCAAA GCTGCACGTC GCCGTTCTTC 
AAGGTGGAGA ATCAGCTGAA GCAGCACCAG CATCTTAATC CCGAGCAGGC TGCCGCTCAG 
CAAAGGGAAA GGGAACGCGA GCAAAGAGAA CGGGAGCAGC AGCAGCGGCT GCGGCACGAC 
GACCAGGACA AGATGGCTCG TCTCTACCAG GAGCTGATTG CACGCACTCC GCGTGAGACG 
GCTTTTCCAA GCTTTCTCTT CTCACCCTCG CTATTTGGAG GAGCCGCCGG AATGCCAGGA 
GCAGCCAGCA ATGCCTTTCC CGCCATGGCC GATGAAAATA TGCGTCACGT CTTTGAACGT 
GAGATTGCCA AGCTGCAGCA GCACCAACAG CAACAACAAG CTGCCCAGGC TCAGGCACAG 
TTCCCCAACT TCTCGAGCCT GATGGCTTTG CAGCAGCAGG TCCTCAACGG AGCCCAGGAT 
CTCTCGCTAG CTGCCGCTGC AGCCAAGGAC ATCAAGTTGA ACGGTCAGCG ATCCTCGTTG 
GAGCACAGTG CCGGCAGCAG CAGTTGTTCA AAAGATGGCG AACGAGATGA TGCGTATCCA 
AGTTCCCTAC ATGGTCGCAA GTCCGAGGGA GGTGGCACAC CCGCACCACC TGCCCCACCA 
TCTGGCCCAG GAACCGGAGC AGGAGCCCCA CCCACAGCAG CACCACCAAC CGGCGGTGCC 
AGCAGCAATT CGGCAGCACC CAGTCCGCTA AGCAATTCAA TACTTCCACC GGCATTGAGC 
AGCCAAGGTG AGGAGTTCGC CGCGACAGCA AGTCCTTTGC AGCGAATGGC CTCGATCACC 
AATTCGCTGA TTACCCAACC ACCGGTGACG CCGCACCACA GTACGCCACA GCGACCCACC 
AAGGCGGTTC TGCCACCGAT CACCCAGCAA CAGTTCGATA TGTTCAACAA CCTTAACACC 
GAGGATATAG TCAGACGTGT GAAGGAGGCC CTGTCGCAGT ACTCCATCTC CCAGCGATTG 
TTCGGCGAGT CCGTGCTGGG TTTGTCTCAG GGATCGGTCT CCGATTTATT GGCTAGACCC 
AAGCCATGGC ACATGCTCAC TCAAAAGGGC CGTGAACCCT TCATTCGCAT GAAGATGTTC 
CTGGAGGATG AGAACGCGGT GCACAAACTG GTGGCCAGTC AATACAAGAT TGCCCCCGAG 
AAGCTAATGC GAACGGGCAG TTACAGTGGA AGTCCGCAGA TGCCACAGGG ATTGGCAAGC 
AAAATGCAGG CTGCCTCCCT GCCCATGCAG AAGATGATGA GCGAACTTAA GCTGCAGGAG 
CCAGCACAGG CGCAGCACTT GATGCAGCAA ATGCAGGCGG CGGCAATGTC GGCAGCAATG 
CAACAGCAGC AAGTGGCACA AGCGCAGCAG CAGGCTCAGC AGGCGCAACA GGCACAACAG 
CATTTGCAGC AGCAGGCGCA GCAGCACTTG CAACAGCAGC AACATCTTGC CCAGCAGCAA 
CACCCCCATC AGCAGCACCA TCAAGCGGCC GCCGCGGCTG CCGCATTGCA CCACCAAAGC 
ATGCTTCTGA CCTCGCCCGG ACTGCCACCC CAGCATGCCA TCAGTCTGCC ACCTTCAGCG 
GGTGGAGCCC AACCAGGTGG TCCTGGTGGC AATCAGGGCA GCTCAAATCC CTCAAACAGC 
GAAAAGAAAC CCATGCTGAT GCCGGTTCAT GGCACTAATG CCATGAGAAG TCTGCACCAG 
CACATGTCGC CCACCGTTTA CGAAATGGCC GCTTTAACCC AAGATCTGGA CACCCACGAT 
ATCACTACCA AGATCAAGGA GGCGTTGTTG GCCAACAATA TTGGTCAGAA AATATTTGGC 
GAAGCCGTTT TGGGACTTTC GCAGGGATCC GTATCTGAGC TGCTGAGCAA ACCGAAGCCC 
TGGCACATGC TCTCCATCAA GGGCAGGGAA CCCTTCATCC GGATGCAATT GTGGCTCAGT 
GATGCCAACA ATGTTGAACG ATTGCAGTTG CTGAAAAATG AGCGACGGGA GGCCAGCAAA 
CGAAGGAGGA GCACAGGCCC AAACCAACAG GACAATAGTA GCGATACCTC CAGCAATGAT 
ACCAATGACT TCTATACCAG CAGTCCGGGT CCAGGATCCG TCGGTTCGGG TGTCGGTGGT 
GCTCCGCCGA GCAAAAAACA GCGTGTCCTC TTCTCCGAGG AGCAAAAGGA GGCACTTCGG 
CTGGCATTCG CCTTGGATCC GTATCCAAAT GTGGGGACCA TCGAGTTTCT GGCCAATGAG 
CTGGGCTTGG CAACGCGGAC GATCACCAAC TGGTTCCACA ATCATCGCAT GCGATTGAAG 
CAACAGGTGC CACACGGACC TGCCGGTCAA GATAATCCGA TACCGAGTCG CGAGAGTACC 
AGTGCCACGC CCTTCGATCC CGTCCAATTC CGCATTCTGC TGCAGCAGCG TCTACTGGAG 
CTCCACAAGG AGCGAATGGG CATGAGCGGT GCCCCCATTC CCTATCCGCC GTACTTTGCT 
GCCGCTGCCA TTTTGGGTCG CAGTCTGGCG GGGATTCCCG GTGCGGCAGC GGCCGCTGGA 
GCAGCTGCAG CAGCCGCCGC AGTTGGCGCC TCTGGTGGTG ATGAGTTGCA GGCTCTGAAT 
CAGGCCTTCA AGGAGCAGAT GAGCGGTTTG GATCTGTCGA TGCCCACCTT AAAGCGGGAA 
CGCAGCGATG ACTATCAGGA CGATCTGGAA CTGGAGGGTG GTGGTCACAA TCTAAGTGAC 
AACGAATCGC TGGAGGGTCA AGAGCCGGAG GACAAGACCA CCGATTACGA GAAGGTGCTG 
CACAAATCCG CTTTGGCCGC TGCCGCCGCT TACATGTCCA ATGCAGTGCG GAGTTCACGA 
CGAAAGCCAG CTGCCCCACA GTGGGTCAAT CCCGCCGGAG CGGTGACCAA TCCAAGTGCT 
GTAGTTGCAG CCGTTGCTGC TGCCGCAGCT GCCGCGGCGG ACAATGAACG CATCATCAAC 
GGGGTGTGCG TGATGCAGGC CTCCGAGTAC GGCCGCGATG ATACTGATAG CAACAAGCCA 
ACGGACGGCG GCAACGATTC CGATCATGAG CACGCCCAGC TGGAGATCGA TCAGCGTTTC 
ATGGAACCGG AGGTGCACAT CAAACAGGAG GAGGACGACG ATGAGGAGCA ATCGGGATCG 
GTTAATCTGG ACAACGAGGA CAATGCGACT AGCGAGCAAA AACTGAAGGT GATCAACGAG 
GAGAAGCTGC GAATGGTGCG AGTTCGCCGG CTGAGCAGCA CTGGTGGTGG TTCCTCTGAG 
GAAATGCCAG CTCCCCTCGC ACCACCGCCT CCTCCGCCAG CAGCTTCTTC TTCTATCGTT 
AGTGGAGAAA GCACCACCAG CAGCAGTAGT AGCAGCAACA CCAGCAGCAG CACACCGGCG 
GTAACCACTG CAGCAGCAAC TGCGGCAGCC GGTTGGAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259310.1
CDS	989..7513
Translation	MQPTLPQAAGTADMDLTAVQSINDWFFKKEQIYLLAQFWQQRATLAEKEVNTLKEQLSTGNPDSNLNSENSDTAAAAATAAAVAAVVAGATATNDIEDEQQQQLQQTASGGILESDSDKLLNSSIVAAAITLQQQNGSNLLANTNTPSPSPPLLSAEQQQQLQSSLQQSGGVGGACLNPKLFFNHAQQMMMMEAAAAAAAAALQQQQQQQSPLHSPANEVAIPTEQPAATVATGAAAAAAAAATPIATGNVKSGSTTSNANHTNSNNSHQDEEELDDEEEDEEEDEDEDDEEENASMQSNADDMELDAQQETRTEPSATTQQQHQQQDTEDLEENKDAGEASLNVSNNHNTTDSNNSCSRKNNNGGNESEQHVASSAEDDDCANNNTNTSNNNNTSNTATSNTNNNNNNNSSSGNSEKRKKKNNNNNNGQPAVLLAAKDKEIKALLDELQRLRAQEQTHLVQIQRLEEHLEVKRQHIIRLEARLDKQQINEALAEATALSAAASTNNNNNSQSSDNNKKLNTAAERPMDASSNADLPESTKAPVPAEDDEEDEDQAMLVDSEEAEDKPEDSHHDDDEDEDEDREAVNATTTDSNELKIKKEQHPLDLNVLSPNSAIAAAAAAAAAAACANDPNKFQALLIERTKALAAEALKNGASDALSEDAHHQQQQHHQQQHQHQQQHHQQQHLHQQHHHHLQQQPNSGSNSNPASNDHHHGHHLHGHGLLHPSSAHHLHHQTTESNSNSSTPTAAGNNNGSNNSSSNTNANSTAQLAASLASTLNGTKSLMQEDSNGLAAVAMAAHAQHAAALGPGFLPGLPAFQFAAAQVAAGGDGRGHYRFADSELQLPPGASMAGRLGESLIPKGDPMEAKLQEMLRYNMDKYANQALDTLHISRRVRELLSVHNIGQRLFAKYILGLSQGTVSELLSKPKPWDKLTEKGRDSYRKMHAWACDDNAVMLLKSLIPKKDSGLPQYAGRGAGGAGGDDSMSEDRIAHILSEASSLMKQSSVAQHREQERRSHGGEDSHSNEDSKSPPQSCTSPFFKVENQLKQHQHLNPEQAAAQQREREREQREREQQQRLRHDDQDKMARLYQELIARTPRETAFPSFLFSPSLFGGAAGMPGAASNAFPAMADENMRHVFEREIAKLQQHQQQQQAAQAQAQFPNFSSLMALQQQVLNGAQDLSLAAAAAKDIKLNGQRSSLEHSAGSSSCSKDGERDDAYPSSLHGRKSEGGGTPAPPAPPSGPGTGAGAPPTAAPPTGGASSNSAAPSPLSNSILPPALSSQGEEFAATASPLQRMASITNSLITQPPVTPHHSTPQRPTKAVLPPITQQQFDMFNNLNTEDIVRRVKEALSQYSISQRLFGESVLGLSQGSVSDLLARPKPWHMLTQKGREPFIRMKMFLEDENAVHKLVASQYKIAPEKLMRTGSYSGSPQMPQGLASKMQAASLPMQKMMSELKLQEPAQAQHLMQQMQAAAMSAAMQQQQVAQAQQQAQQAQQAQQHLQQQAQQHLQQQQHLAQQQHPHQQHHQAAAAAAALHHQSMLLTSPGLPPQHAISLPPSAGGAQPGGPGGNQGSSNPSNSEKKPMLMPVHGTNAMRSLHQHMSPTVYEMAALTQDLDTHDITTKIKEALLANNIGQKIFGEAVLGLSQGSVSELLSKPKPWHMLSIKGREPFIRMQLWLSDANNVERLQLLKNERREASKRRRSTGPNQQDNSSDTSSNDTNDFYTSSPGPGSVGSGVGGAPPSKKQRVLFSEEQKEALRLAFALDPYPNVGTIEFLANELGLATRTITNWFHNHRMRLKQQVPHGPAGQDNPIPSRESTSATPFDPVQFRILLQQRLLELHKERMGMSGAPIPYPPYFAAAAILGRSLAGIPGAAAAAGAAAAAAAVGASGGDELQALNQAFKEQMSGLDLSMPTLKRERSDDYQDDLELEGGGHNLSDNESLEGQEPEDKTTDYEKVLHKSALAAAAAYMSNAVRSSRRKPAAPQWVNPAGAVTNPSAVVAAVAAAAAAAADNERIINGVCVMQASEYGRDDTDSNKPTDGGNDSDHEHAQLEIDQRFMEPEVHIKQEEDDDEEQSGSVNLDNEDNATSEQKLKVINEEKLRMVRVRRLSSTGGGSSEEMPAPLAPPPPPPAASSSIVSGESTTSSSSSSNTSSSTPAVTTAAATAAAGWNY

Target ORF information:

RefSeq Version	NM_001272381.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cut (ct), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272381.2

ORF Insert Sequence:

ATGCAGCCAA CATTGCCACA AGCCGCTGGG ACAGCCGATA TGGATCTGAC GGCTGTTCAA 
TCAATCAACG ATTGGTTTTT CAAAAAGGAG CAAATTTATT TATTGGCACA GTTTTGGCAA 
CAGCGCGCAA CCCTTGCCGA AAAGGAAGTG AACACACTCA AAGAGCAGCT ATCCACCGGC 
AATCCCGACA GCAACCTCAA CAGCGAAAAC AGCGATACAG CAGCAGCGGC AGCAACAGCA 
GCAGCAGTTG CAGCAGTTGT TGCAGGAGCA ACAGCAACAA ACGACATCGA GGACGAGCAG 
CAGCAACAGT TGCAGCAGAC GGCCAGCGGC GGCATTTTGG AAAGCGACAG CGACAAGCTG 
CTGAACAGCT CCATTGTTGC AGCGGCCATA ACGCTGCAGC AGCAGAACGG CAGCAATCTG 
CTGGCCAACA CAAATACGCC GTCGCCCTCG CCGCCGTTGC TCAGCGCCGA GCAGCAGCAG 
CAGTTGCAGA GCAGTTTGCA ACAGAGCGGC GGTGTTGGCG GCGCCTGCCT CAATCCCAAG 
CTTTTCTTCA ATCACGCCCA ACAGATGATG ATGATGGAGG CGGCAGCCGC GGCAGCAGCG 
GCGGCATTGC AACAGCAGCA GCAACAGCAA TCACCACTCC ATTCGCCGGC GAATGAAGTT 
GCGATTCCCA CAGAACAGCC AGCGGCAACA GTGGCAACAG GAGCCGCTGC TGCAGCAGCC 
GCAGCAGCAA CACCGATTGC AACTGGCAAC GTCAAAAGCG GCAGCACCAC CAGCAACGCC 
AATCACACCA ACAGCAACAA TAGTCACCAG GACGAGGAGG AGCTGGACGA TGAGGAGGAG 
GACGAGGAGG AGGATGAGGA CGAGGATGAT GAGGAGGAGA ATGCCTCGAT GCAATCGAAT 
GCTGATGATA TGGAGCTGGA TGCGCAGCAA GAAACCAGAA CTGAGCCAAG TGCAACAACA 
CAACAGCAGC ATCAGCAGCA GGATACAGAG GATCTTGAGG AGAACAAGGA TGCGGGGGAG 
GCTAGTTTAA ATGTTAGCAA TAATCACAAT ACAACCGATA GCAATAATAG TTGTAGTCGA 
AAGAACAACA ATGGCGGCAA TGAAAGTGAG CAACATGTGG CCAGTTCGGC GGAGGACGAT 
GATTGCGCCA ACAACAATAC AAATACCAGC AATAACAACA ATACCAGCAA CACCGCCACC 
AGCAACACGA ACAACAACAA TAATAATAAC AGCAGCAGCG GCAACAGCGA GAAGCGCAAG 
AAGAAGAACA ATAACAACAA CAACGGCCAG CCTGCTGTTC TATTAGCTGC CAAAGATAAA 
GAGATTAAAG CCCTTTTGGA CGAACTGCAG CGCTTGAGGG CGCAGGAGCA GACGCACCTG 
GTGCAAATCC AACGATTGGA GGAACATCTT GAGGTCAAGC GTCAGCACAT CATCCGATTG 
GAGGCACGTC TGGATAAGCA GCAAATTAAT GAGGCACTGG CCGAGGCGAC CGCTTTGTCC 
GCTGCCGCGT CAACCAACAA CAACAATAAT AGCCAGAGCA GCGACAACAA TAAGAAGCTG 
AATACCGCTG CAGAAAGACC GATGGATGCG TCATCCAATG CCGATCTACC GGAGTCCACA 
AAAGCTCCAG TTCCGGCGGA GGATGACGAA GAAGATGAGG ATCAGGCCAT GCTGGTGGAT 
TCCGAGGAAG CGGAGGACAA GCCAGAGGAC TCACATCACG ATGACGACGA AGATGAGGAC 
GAGGATCGGG AGGCGGTCAA TGCCACCACA ACCGATAGCA ACGAGCTCAA AATCAAAAAG 
GAACAGCATC CGCTGGATCT GAATGTGCTC AGTCCGAATT CGGCCATTGC CGCTGCAGCT 
GCGGCCGCCG CTGCCGCCGC CTGCGCCAAT GACCCCAACA AATTCCAGGC TCTGCTCATC 
GAGCGGACCA AAGCCTTGGC CGCAGAGGCG CTCAAGAATG GCGCCAGCGA TGCCCTCAGC 
GAAGATGCCC ACCATCAGCA GCAGCAGCAC CACCAACAGC AGCACCAACA CCAGCAACAA 
CACCACCAGC AACAGCACCT CCACCAGCAA CATCACCATC ACCTGCAACA GCAACCAAAC 
AGCGGCAGCA ACAGCAATCC AGCGAGCAAC GACCACCATC ATGGACACCA TTTGCATGGC 
CATGGTTTGC TGCATCCTTC GTCGGCGCAC CATCTGCACC ACCAAACGAC CGAATCCAAT 
TCGAATTCGA GTACACCCAC AGCGGCGGGA AATAACAATG GTAGCAATAA CAGCTCCAGC 
AACACAAATG CCAATAGTAC CGCTCAATTG GCCGCCAGTT TGGCCAGTAC ACTCAATGGC 
ACCAAGTCCC TGATGCAGGA GGATAGCAAT GGCTTGGCCG CCGTCGCCAT GGCGGCTCAT 
GCCCAACATG CGGCTGCCTT GGGACCTGGA TTTTTGCCCG GCCTTCCAGC GTTTCAGTTT 
GCCGCCGCCC AAGTGGCCGC CGGTGGCGAT GGACGGGGTC ATTATCGTTT CGCCGACTCG 
GAACTGCAAT TGCCACCGGG TGCTTCGATG GCCGGTCGTC TTGGAGAGTC GCTCATACCC 
AAGGGTGATC CCATGGAGGC CAAACTCCAA GAGATGTTGC GCTACAACAT GGACAAGTAC 
GCCAATCAGG CGCTGGATAC CCTGCACATT TCGCGACGTG TCCGCGAACT GCTATCGGTG 
CACAACATTG GCCAGCGACT ATTTGCCAAA TACATACTGG GCCTGTCGCA GGGCACCGTT 
TCCGAGCTGC TGAGCAAACC CAAGCCGTGG GACAAGTTGA CGGAGAAGGG ACGAGATAGC 
TACCGCAAAA TGCACGCCTG GGCCTGTGAC GATAATGCCG TCATGCTGCT CAAATCGCTG 
ATACCCAAGA AAGATTCTGG ACTGCCGCAG TATGCGGGTC GTGGTGCCGG CGGCGCTGGC 
GGCGATGATT CCATGTCCGA GGATCGGATT GCACATATCC TCAGCGAAGC CTCCTCGCTG 
ATGAAACAGA GCAGTGTGGC CCAGCATCGG GAGCAGGAGC GTCGCAGTCA CGGCGGCGAG 
GATTCACACA GCAACGAGGA CAGCAAATCG CCACCTCAAA GCTGCACGTC GCCGTTCTTC 
AAGGTGGAGA ATCAGCTGAA GCAGCACCAG CATCTTAATC CCGAGCAGGC TGCCGCTCAG 
CAAAGGGAAA GGGAACGCGA GCAAAGAGAA CGGGAGCAGC AGCAGCGGCT GCGGCACGAC 
GACCAGGACA AGATGGCTCG TCTCTACCAG GAGCTGATTG CACGCACTCC GCGTGAGACG 
GCTTTTCCAA GCTTTCTCTT CTCACCCTCG CTATTTGGAG GAGCCGCCGG AATGCCAGGA 
GCAGCCAGCA ATGCCTTTCC CGCCATGGCC GATGAAAATA TGCGTCACGT CTTTGAACGT 
GAGATTGCCA AGCTGCAGCA GCACCAACAG CAACAACAAG CTGCCCAGGC TCAGGCACAG 
TTCCCCAACT TCTCGAGCCT GATGGCTTTG CAGCAGCAGG TCCTCAACGG AGCCCAGGAT 
CTCTCGCTAG CTGCCGCTGC AGCCAAGGAC ATCAAGTTGA ACGGTCAGCG ATCCTCGTTG 
GAGCACAGTG CCGGCAGCAG CAGTTGTTCA AAAGATGGCG AACGAGATGA TGCGTATCCA 
AGTTCCCTAC ATGGTCGCAA GTCCGAGGGA GGTGGCACAC CCGCACCACC TGCCCCACCA 
TCTGGCCCAG GAACCGGAGC AGGAGCCCCA CCCACAGCAG CACCACCAAC CGGCGGTGCC 
AGCAGCAATT CGGCAGCACC CAGTCCGCTA AGCAATTCAA TACTTCCACC GGCATTGAGC 
AGCCAAGGTG AGGAGTTCGC CGCGACAGCA AGTCCTTTGC AGCGAATGGC CTCGATCACC 
AATTCGCTGA TTACCCAACC ACCGGTGACG CCGCACCACA GTACGCCACA GCGACCCACC 
AAGGCGGTTC TGCCACCGAT CACCCAGCAA CAGTTCGATA TGTTCAACAA CCTTAACACC 
GAGGATATAG TCAGACGTGT GAAGGAGGCC CTGTCGCAGT ACTCCATCTC CCAGCGATTG 
TTCGGCGAGT CCGTGCTGGG TTTGTCTCAG GGATCGGTCT CCGATTTATT GGCTAGACCC 
AAGCCATGGC ACATGCTCAC TCAAAAGGGC CGTGAACCCT TCATTCGCAT GAAGATGTTC 
CTGGAGGATG AGAACGCGGT GCACAAACTG GTGGCCAGTC AATACAAGAT TGCCCCCGAG 
AAGCTAATGC GAACGGGCAG TTACAGTGGA AGTCCGCAGA TGCCACAGGG ATTGGCAAGC 
AAAATGCAGG CTGCCTCCCT GCCCATGCAG AAGATGATGA GCGAACTTAA GCTGCAGGAG 
CCAGCACAGG CGCAGCACTT GATGCAGCAA ATGCAGGCGG CGGCAATGTC GGCAGCAATG 
CAACAGCAGC AAGTGGCACA AGCGCAGCAG CAGGCTCAGC AGGCGCAACA GGCACAACAG 
CATTTGCAGC AGCAGGCGCA GCAGCACTTG CAACAGCAGC AACATCTTGC CCAGCAGCAA 
CACCCCCATC AGCAGCACCA TCAAGCGGCC GCCGCGGCTG CCGCATTGCA CCACCAAAGC 
ATGCTTCTGA CCTCGCCCGG ACTGCCACCC CAGCATGCCA TCAGTCTGCC ACCTTCAGCG 
GGTGGAGCCC AACCAGGTGG TCCTGGTGGC AATCAGGGCA GCTCAAATCC CTCAAACAGC 
GAAAAGAAAC CCATGCTGAT GCCGGTTCAT GGCACTAATG CCATGAGAAG TCTGCACCAG 
CACATGTCGC CCACCGTTTA CGAAATGGCC GCTTTAACCC AAGATCTGGA CACCCACGAT 
ATCACTACCA AGATCAAGGA GGCGTTGTTG GCCAACAATA TTGGTCAGAA AATATTTGGC 
GAAGCCGTTT TGGGACTTTC GCAGGGATCC GTATCTGAGC TGCTGAGCAA ACCGAAGCCC 
TGGCACATGC TCTCCATCAA GGGCAGGGAA CCCTTCATCC GGATGCAATT GTGGCTCAGT 
GATGCCAACA ATGTTGAACG ATTGCAGTTG CTGAAAAATG AGCGACGGGA GGCCAGCAAA 
CGAAGGAGGA GCACAGGCCC AAACCAACAG GACAATAGTA GCGATACCTC CAGCAATGAT 
ACCAATGACT TCTATACCAG CAGTCCGGGT CCAGGATCCG TCGGTTCGGG TGTCGGTGGT 
GCTCCGCCGA GCAAAAAACA GCGTGTCCTC TTCTCCGAGG AGCAAAAGGA GGCACTTCGG 
CTGGCATTCG CCTTGGATCC GTATCCAAAT GTGGGGACCA TCGAGTTTCT GGCCAATGAG 
CTGGGCTTGG CAACGCGGAC GATCACCAAC TGGTTCCACA ATCATCGCAT GCGATTGAAG 
CAACAGGTGC CACACGGACC TGCCGGTCAA GATAATCCGA TACCGAGTCG CGAGAGTACC 
AGTGCCACGC CCTTCGATCC CGTCCAATTC CGCATTCTGC TGCAGCAGCG TCTACTGGAG 
CTCCACAAGG AGCGAATGGG CATGAGCGGT GCCCCCATTC CCTATCCGCC GTACTTTGCT 
GCCGCTGCCA TTTTGGGTCG CAGTCTGGCG GGGATTCCCG GTGCGGCAGC GGCCGCTGGA 
GCAGCTGCAG CAGCCGCCGC AGTTGGCGCC TCTGGTGGTG ATGAGTTGCA GGCTCTGAAT 
CAGGCCTTCA AGGAGCAGAT GAGCGGTTTG GATCTGTCGA TGCCCACCTT AAAGCGGGAA 
CGCAGCGATG ACTATCAGGA CGATCTGGAA CTGGAGGGTG GTGGTCACAA TCTAAGTGAC 
AACGAATCGC TGGAGGGTCA AGAGCCGGAG GACAAGACCA CCGATTACGA GAAGGTGCTG 
CACAAATCCG CTTTGGCCGC TGCCGCCGCT TACATGTCCA ATGCAGTGCG GAGTTCACGA 
CGAAAGCCAG CTGCCCCACA GTGGGTCAAT CCCGCCGGAG CGGTGACCAA TCCAAGTGCT 
GTAGTTGCAG CCGTTGCTGC TGCCGCAGCT GCCGCGGCGG ACAATGAACG CATCATCAAC 
GGGGTGTGCG TGATGCAGGC CTCCGAGTAC GGCCGCGATG ATACTGATAG CAACAAGCCA 
ACGGACGGCG GCAACGATTC CGATCATGAG CACGCCCAGC TGGAGATCGA TCAGCGTTTC 
ATGGAACCGG AGGTGCACAT CAAACAGGAG GAGGACGACG ATGAGGAGCA ATCGGGATCG 
GTTAATCTGG ACAACGAGGA CAATGCGACT AGCGAGCAAA AACTGAAGGT GATCAACGAG 
GAGAAGCTGC GAATGGTGCG AGTTCGCCGG CTGAGCAGCA CTGGTGGTGG TTCCTCTGAG 
GAAATGCCAG CTCCCCTCGC ACCACCGCCT CCTCCGCCAG CAGCTTCTTC TTCTATCGTT 
AGTGGAGAAA GCACCACCAG CAGCAGTAGT AGCAGCAACA CCAGCAGCAG CACACCGGCG 
GTAACCACTG CAGCAGCAAC TGCGGCAGCC GGTTGGAACT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ct ( NM_001258636.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245565.1 Drosophila melanogaster cut (ct), transcript variant D, mRNA.

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CloneID	OFa03736
Clone ID Related Accession (Same CDS sequence)	NM_001258636.2
Accession Version	NM_001258636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	cut, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258636.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGATGA TGGAGGCGGC AGCCGCGGCA GCAGCGGCGG CATTGCAACA GCAGCAGCAA 
CAGCAATCAC CACTCCATTC GCCGGCGAAT GAAGTTGCGA TTCCCACAGA ACAGCCAGCG 
GCAACAGTGG CAACAGGAGC CGCTGCTGCA GCAGCCGCAG CAGCAACACC GATTGCAACT 
GGCAACGTCA AAAGCGGCAG CACCACCAGC AACGCCAATC ACACCAACAG CAACAATAGT 
CACCAGGACG AGGAGGAGCT GGACGATGAG GAGGAGGACG AGGAGGAGGA TGAGGACGAG 
GATGATGAGG AGGAGAATGC CTCGATGCAA TCGAATGCTG ATGATATGGA GCTGGATGCG 
CAGCAAGAAA CCAGAACTGA GCCAAGTGCA ACAACACAAC AGCAGCATCA GCAGCAGGAT 
ACAGAGGATC TTGAGGAGAA CAAGGATGCG GGGGAGGCTA GTTTAAATGT TAGCAATAAT 
CACAATACAA CCGATAGCAA TAATAGTTGT AGTCGAAAGA ACAACAATGG CGGCAATGAA 
AGTGAGCAAC ATGTGGCCAG TTCGGCGGAG GACGATGATT GCGCCAACAA CAATACAAAT 
ACCAGCAATA ACAACAATAC CAGCAACACC GCCACCAGCA ACACGAACAA CAACAATAAT 
AATAACAGCA GCAGCGGCAA CAGCGAGAAG CGCAAGAAGA AGAACAATAA CAACAACAAC 
GGCCAGCCTG CTGTTCTATT AGCTGCCAAA GATAAAGAGA TTAAAGCCCT TTTGGACGAA 
CTGCAGCGCT TGAGGGCGCA GGAGCAGACG CACCTGGTGC AAATCCAACG ATTGGAGGAA 
CATCTTGAGG TCAAGCGTCA GCACATCATC CGATTGGAGG CACGTCTGGA TAAGCAGCAA 
ATTAATGAGG CACTGGCCGA GGCGACCGCT TTGTCCGCTG CCGCGTCAAC CAACAACAAC 
AATAATAGCC AGAGCAGCGA CAACAATAAG AAGCTGAATA CCGCTGCAGA AAGACCGATG 
GATGCGTCAT CCAATGCCGA TCTACCGGAG TCCACAAAAG CTCCAGTTCC GGCGGAGGAT 
GACGAAGAAG ATGAGGATCA GGCCATGCTG GTGGATTCCG AGGAAGCGGA GGACAAGCCA 
GAGGACTCAC ATCACGATGA CGACGAAGAT GAGGACGAGG ATCGGGAGGC GGTCAATGCC 
ACCACAACCG ATAGCAACGA GCTCAAAATC AAAAAGGAAC AGCACAGTCC GCTGGATCTG 
AATGTGCTCA GTCCGAATTC GGCCATTGCC GCTGCAGCTG CGGCCGCCGC TGCCGCCGCC 
TGCGCCAATG ACCCCAACAA ATTCCAGGCT CTGCTCATCG AGCGGACCAA AGCCTTGGCC 
GCAGAGGCGC TCAAGAATGG CGCCAGCGAT GCCCTCAGCG AAGAAACCAA CCGAAATCAT 
GAAGACGAAG ACGAAGTGTC GCCAGCAGCA ACTGCAACTG CAACACTCGC AACGCCTGCA 
ACAGTGGCAA CACCAATTGA AACACTGGCC ACCGATTCGG AAGCAAAAGC CAGCAATGAC 
ATGGCGCCCA AGCAGGAAAT CGAAGAAGTG GATGACGAGG ATGCCACCGA TGAACCGGTG 
ATCCTGCCGG AGCAACTAGT CAACGACTAT TGGCGACGGG GCTTTGTCGA ATCGGGGAAT 
AGCACTAATA CGAGCACCGC TGCAATGCCA GGGAGCAAAA CGCCAACAAT TTATCAAAAT 
TTTAAGGTCG AGCAGCAGGC ACAGTTGCTG CCGCCACCGC CGCCGCCACC ACCACCCCCG 
CAGCAACAAC ATCAACAGCA GCAACAACAA CAACATCTAC ACTTTTCACA GCAACAGCAA 
CAACAACATC AGCAGCAACA TGGACCATCG TCCACAGCCC TGCTTAGTCA ACTGGTTAAC 
TCAACATTTT CCAACTCTTC CAACTCTTCT CGCCTAGATG CCCACCATCA GCAGCAGCAG 
CACCACCAAC AGCAGCACCA ACACCAGCAA CAACACCACC AGCAACAGCA CCTCCACCAG 
CAACATCACC ATCACCTGCA ACAGCAACCA AACAGCGGCA GCAACAGCAA TCCAGCGAGC 
AACGACCACC ATCATGGACA CCATTTGCAT GGCCATGGTT TGCTGCATCC TTCGTCGGCG 
CACCATCTGC ACCACCAAAC GACCGAATCC AATTCGAATT CGAGTACACC CACAGCGGCG 
GGAAATAACA ATGGTAGCAA TAACAGCTCC AGCAACACAA ATGCCAATAG TACCGCTCAA 
TTGGCCGCCA GTTTGGCCAG TACACTCAAT GGCACCAAGT CCCTGATGCA GGAGGATAGC 
AATGGCTTGG CCGCCGTCGC CATGGCGGCT CATGCCCAAC ATGCGGCTGC CTTGGGACCT 
GGATTTTTGC CCGGCCTTCC AGCGTTTCAG TTTGCCGCCG CCCAAGTGGC CGCCGGTGGC 
GATGGACGGG GTCATTATCG TTTCGCCGAC TCGGAACTGC AATTGCCACC GGGTGCTTCG 
ATGGCCGGTC GTCTTGGAGA GTCGCTCATA CCCAAGGGTG ATCCCATGGA GGCCAAACTC 
CAAGAGATGT TGCGCTACAA CATGGACAAG TACGCCAATC AGGCGCTGGA TACCCTGCAC 
ATTTCGCGAC GTGTCCGCGA ACTGCTATCG GTGCACAACA TTGGCCAGCG ACTATTTGCC 
AAATACATAC TGGGCCTGTC GCAGGGCACC GTTTCCGAGC TGCTGAGCAA ACCCAAGCCG 
TGGGACAAGT TGACGGAGAA GGGACGAGAT AGCTACCGCA AAATGCACGC CTGGGCCTGT 
GACGATAATG CCGTCATGCT GCTCAAATCG CTGATACCCA AGAAAGATTC TGGACTGCCG 
CAGTATGCGG GTCGTGGTGC CGGCGGCGCT GGCGGCGATG ATTCCATGTC CGAGGATCGG 
ATTGCACATA TCCTCAGCGA AGCCTCCTCG CTGATGAAAC AGAGCAGTGT GGCCCAGCAT 
CGGGAGCAGG AGCGTCGCAG TCACGGCGGC GAGGATTCAC ACAGCAACGA GGACAGCAAA 
TCGCCACCTC AAAGCTGCAC GTCGCCGTTC TTCAAGGTGG AGAATCAGCT GAAGCAGCAC 
CAGCATCTTA ATCCCGAGCA GGCTGCCGCT CAGCAAAGGG AAAGGGAACG CGAGCAAAGA 
GAACGGGAGC AGCAGCAGCG GCTGCGGCAC GACGACCAGG ACAAGATGGC TCGTCTCTAC 
CAGGAGCTGA TTGCACGCAC TCCGCGTGAG ACGGCTTTTC CAAGCTTTCT CTTCTCACCC 
TCGCTATTTG GAGGAGCCGC CGGAATGCCA GGAGCAGCCA GCAATGCCTT TCCCGCCATG 
GCCGATGAAA ATATGCGTCA CGTCTTTGAA CGTGAGATTG CCAAGCTGCA GCAGCACCAA 
CAGCAACAAC AAGCTGCCCA GGCTCAGGCA CAGTTCCCCA ACTTCTCGAG CCTGATGGCT 
TTGCAGCAGC AGGTCCTCAA CGGAGCCCAG GATCTCTCGC TAGCTGCCGC TGCAGCCAAG 
GACATCAAGT TGAACGGTCA GCGATCCTCG TTGGAGCACA GTGCCGGCAG CAGCAGTTGT 
TCAAAAGATG GCGAACGAGA TGATGCGTAT CCAAGTTCCC TACATGGTCG CAAGTCCGAG 
GGAGGTGGCA CACCCGCACC ACCTGCCCCA CCATCTGGCC CAGGAACCGG AGCAGGAGCC 
CCACCCACAG CAGCACCACC AACCGGCGGT GCCAGCAGCA ATTCGGCAGC ACCCAGTCCG 
CTAAGCAATT CAATACTTCC ACCGGCATTG AGCAGCCAAG GTGAGGAGTT CGCCGCGACA 
GCAAGTCCTT TGCAGCGAAT GGCCTCGATC ACCAATTCGC TGATTACCCA ACCACCGGTG 
ACGCCGCACC ACAGTACGCC ACAGCGACCC ACCAAGGCGG TTCTGCCACC GATCACCCAG 
CAACAGTTCG ATATGTTCAA CAACCTTAAC ACCGAGGATA TAGTCAGACG TGTGAAGGAG 
GCCCTGTCGC AGTACTCCAT CTCCCAGCGA TTGTTCGGCG AGTCCGTGCT GGGTTTGTCT 
CAGGGATCGG TCTCCGATTT ATTGGCTAGA CCCAAGCCAT GGCACATGCT CACTCAAAAG 
GGCCGTGAAC CCTTCATTCG CATGAAGATG TTCCTGGAGG ATGAGAACGC GGTGCACAAA 
CTGGTGGCCA GTCAATACAA GATTGCCCCC GAGAAGCTAA TGCGAACGGG CAGTTACAGT 
GGAAGTCCGC AGATGCCACA GGGATTGGCA AGCAAAATGC AGGCTGCCTC CCTGCCCATG 
CAGAAGATGA TGAGCGAACT TAAGCTGCAG GAGCCAGCAC AGGCGCAGCA CTTGATGCAG 
CAAATGCAGG CGGCGGCAAT GTCGGCAGCA ATGCAACAGC AGCAAGTGGC ACAAGCGCAG 
CAGCAGGCTC AGCAGGCGCA ACAGGCACAA CAGCATTTGC AGCAGCAGGC GCAGCAGCAC 
TTGCAACAGC AGCAACATCT TGCCCAGCAG CAACACCCCC ATCAGCAGCA CCATCAAGCG 
GCCGCCGCGG CTGCCGCATT GCACCACCAA AGCATGCTTC TGACCTCGCC CGGACTGCCA 
CCCCAGCATG CCATCAGTCT GCCACCTTCA GCGGGTGGAG CCCAACCAGG TGGTCCTGGT 
GGCAATCAGG GCAGCTCAAA TCCCTCAAAC AGCGAAAAGA AACCCATGCT GATGCCGGTT 
CATGGCACTA ATGCCATGAG AAGTCTGCAC CAGCACATGT CGCCCACCGT TTACGAAATG 
GCCGCTTTAA CCCAAGATCT GGACACCCAC GATATCACTA CCAAGATCAA GGAGGCGTTG 
TTGGCCAACA ATATTGGTCA GAAAATATTT GGCGAAGCCG TTTTGGGACT TTCGCAGGGA 
TCCGTATCTG AGCTGCTGAG CAAACCGAAG CCCTGGCACA TGCTCTCCAT CAAGGGCAGG 
GAACCCTTCA TCCGGATGCA ATTGTGGCTC AGTGATGCCA ACAATGTTGA ACGATTGCAG 
TTGCTGAAAA ATGAGCGACG GGAGGCCAGC AAACGAAGGA GGAGCACAGG CCCAAACCAA 
CAGGACAATA GTAGCGATAC CTCCAGCAAT GATACCAATG ACTTCTATAC CAGCAGTCCG 
GGTCCAGGAT CCGTCGGTTC GGGTGTCGGT GGTGCTCCGC CGAGCAAAAA ACAGCGTGTC 
CTCTTCTCCG AGGAGCAAAA GGAGGCACTT CGGCTGGCAT TCGCCTTGGA TCCGTATCCA 
AATGTGGGGA CCATCGAGTT TCTGGCCAAT GAGCTGGGCT TGGCAACGCG GACGATCACC 
AACTGGTTCC ACAATCATCG CATGCGATTG AAGCAACAGG TGCCACACGG ACCTGCCGGT 
CAAGATAATC CGATACCGAG TCGCGAGAGT ACCAGTGCCA CGCCCTTCGA TCCCGTCCAA 
TTCCGCATTC TGCTGCAGCA GCGTCTACTG GAGCTCCACA AGGAGCGAAT GGGCATGAGC 
GGTGCCCCCA TTCCCTATCC GCCGTACTTT GCTGCCGCTG CCATTTTGGG TCGCAGTCTG 
GCGGGGATTC CCGGTGCGGC AGCGGCCGCT GGAGCAGCTG CAGCAGCCGC CGCAGTTGGC 
GCCTCTGGTG GTGATGAGTT GCAGGCTCTG AATCAGGCCT TCAAGGAGCA GATGAGCGGT 
TTGGATCTGT CGATGCCCAC CTTAAAGCGG GAACGCAGCG ATGACTATCA GGACGATCTG 
GAACTGGAGG GTGGTGGTCA CAATCTAAGT GACAACGAAT CGCTGGAGGG TCAAGAGCCG 
GAGGACAAGA CCACCGATTA CGAGAAGGTG CTGCACAAAT CCGCTTTGGC CGCTGCCGCC 
GCTTACATGT CCAATGCAGT GCGGAGTTCA CGACGAAAGC CAGCTGCCCC ACAGTGGGTC 
AATCCCGCCG GAGCGGTGAC CAATCCAAGT GCTGTAGTTG CAGCCGTTGC TGCTGCCGCA 
GCTGCCGCGG CGGACAATGA ACGCATCATC AACGGGGTGT GCGTGATGCA GGCCTCCGAG 
TACGGCCGCG ATGATACTGA TAGCAACAAG CCAACGGACG GCGGCAACGA TTCCGATCAT 
GAGCACGCCC AGCTGGAGAT CGATCAGCGT TTCATGGAAC CGGAGGTGCA CATCAAACAG 
GAGGAGGACG ACGATGAGGA GCAATCGGGA TCGGTTAATC TGGACAACGA GGACAATGCG 
ACTAGCGAGC AAAAACTGAA GGTGATCAAC GAGGAGAAGC TGCGAATGGT GCGAGTTCGC 
CGGCTGAGCA GCACTGGTGG TGGTTCCTCT GAGGAAATGC CAGCTCCCCT CGCACCACCG 
CCTCCTCCGC CAGCAGCTTC TTCTTCTATC GTTAGTGGAG AAAGCACCAC CAGCAGCAGT 
AGTAGCAGCA ACACCAGCAG CAGCACACCG GCGGTAACCA CTGCAGCAGC AACTGCGGCA 
GCCGGTTGGA ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245565.1
CDS	593..7090
Translation	MMMMEAAAAAAAAALQQQQQQQSPLHSPANEVAIPTEQPAATVATGAAAAAAAAATPIATGNVKSGSTTSNANHTNSNNSHQDEEELDDEEEDEEEDEDEDDEEENASMQSNADDMELDAQQETRTEPSATTQQQHQQQDTEDLEENKDAGEASLNVSNNHNTTDSNNSCSRKNNNGGNESEQHVASSAEDDDCANNNTNTSNNNNTSNTATSNTNNNNNNNSSSGNSEKRKKKNNNNNNGQPAVLLAAKDKEIKALLDELQRLRAQEQTHLVQIQRLEEHLEVKRQHIIRLEARLDKQQINEALAEATALSAAASTNNNNNSQSSDNNKKLNTAAERPMDASSNADLPESTKAPVPAEDDEEDEDQAMLVDSEEAEDKPEDSHHDDDEDEDEDREAVNATTTDSNELKIKKEQHSPLDLNVLSPNSAIAAAAAAAAAAACANDPNKFQALLIERTKALAAEALKNGASDALSEETNRNHEDEDEVSPAATATATLATPATVATPIETLATDSEAKASNDMAPKQEIEEVDDEDATDEPVILPEQLVNDYWRRGFVESGNSTNTSTAAMPGSKTPTIYQNFKVEQQAQLLPPPPPPPPPPQQQHQQQQQQQHLHFSQQQQQQHQQQHGPSSTALLSQLVNSTFSNSSNSSRLDAHHQQQQHHQQQHQHQQQHHQQQHLHQQHHHHLQQQPNSGSNSNPASNDHHHGHHLHGHGLLHPSSAHHLHHQTTESNSNSSTPTAAGNNNGSNNSSSNTNANSTAQLAASLASTLNGTKSLMQEDSNGLAAVAMAAHAQHAAALGPGFLPGLPAFQFAAAQVAAGGDGRGHYRFADSELQLPPGASMAGRLGESLIPKGDPMEAKLQEMLRYNMDKYANQALDTLHISRRVRELLSVHNIGQRLFAKYILGLSQGTVSELLSKPKPWDKLTEKGRDSYRKMHAWACDDNAVMLLKSLIPKKDSGLPQYAGRGAGGAGGDDSMSEDRIAHILSEASSLMKQSSVAQHREQERRSHGGEDSHSNEDSKSPPQSCTSPFFKVENQLKQHQHLNPEQAAAQQREREREQREREQQQRLRHDDQDKMARLYQELIARTPRETAFPSFLFSPSLFGGAAGMPGAASNAFPAMADENMRHVFEREIAKLQQHQQQQQAAQAQAQFPNFSSLMALQQQVLNGAQDLSLAAAAAKDIKLNGQRSSLEHSAGSSSCSKDGERDDAYPSSLHGRKSEGGGTPAPPAPPSGPGTGAGAPPTAAPPTGGASSNSAAPSPLSNSILPPALSSQGEEFAATASPLQRMASITNSLITQPPVTPHHSTPQRPTKAVLPPITQQQFDMFNNLNTEDIVRRVKEALSQYSISQRLFGESVLGLSQGSVSDLLARPKPWHMLTQKGREPFIRMKMFLEDENAVHKLVASQYKIAPEKLMRTGSYSGSPQMPQGLASKMQAASLPMQKMMSELKLQEPAQAQHLMQQMQAAAMSAAMQQQQVAQAQQQAQQAQQAQQHLQQQAQQHLQQQQHLAQQQHPHQQHHQAAAAAAALHHQSMLLTSPGLPPQHAISLPPSAGGAQPGGPGGNQGSSNPSNSEKKPMLMPVHGTNAMRSLHQHMSPTVYEMAALTQDLDTHDITTKIKEALLANNIGQKIFGEAVLGLSQGSVSELLSKPKPWHMLSIKGREPFIRMQLWLSDANNVERLQLLKNERREASKRRRSTGPNQQDNSSDTSSNDTNDFYTSSPGPGSVGSGVGGAPPSKKQRVLFSEEQKEALRLAFALDPYPNVGTIEFLANELGLATRTITNWFHNHRMRLKQQVPHGPAGQDNPIPSRESTSATPFDPVQFRILLQQRLLELHKERMGMSGAPIPYPPYFAAAAILGRSLAGIPGAAAAAGAAAAAAAVGASGGDELQALNQAFKEQMSGLDLSMPTLKRERSDDYQDDLELEGGGHNLSDNESLEGQEPEDKTTDYEKVLHKSALAAAAAYMSNAVRSSRRKPAAPQWVNPAGAVTNPSAVVAAVAAAAAAAADNERIINGVCVMQASEYGRDDTDSNKPTDGGNDSDHEHAQLEIDQRFMEPEVHIKQEEDDDEEQSGSVNLDNEDNATSEQKLKVINEEKLRMVRVRRLSSTGGGSSEEMPAPLAPPPPPPAASSSIVSGESTTSSSSSSNTSSSTPAVTTAAATAAAGWNY

Target ORF information:

RefSeq Version	NM_001258636.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster cut (ct), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258636.2

ORF Insert Sequence:

ATGATGATGA TGGAGGCGGC AGCCGCGGCA GCAGCGGCGG CATTGCAACA GCAGCAGCAA 
CAGCAATCAC CACTCCATTC GCCGGCGAAT GAAGTTGCGA TTCCCACAGA ACAGCCAGCG 
GCAACAGTGG CAACAGGAGC CGCTGCTGCA GCAGCCGCAG CAGCAACACC GATTGCAACT 
GGCAACGTCA AAAGCGGCAG CACCACCAGC AACGCCAATC ACACCAACAG CAACAATAGT 
CACCAGGACG AGGAGGAGCT GGACGATGAG GAGGAGGACG AGGAGGAGGA TGAGGACGAG 
GATGATGAGG AGGAGAATGC CTCGATGCAA TCGAATGCTG ATGATATGGA GCTGGATGCG 
CAGCAAGAAA CCAGAACTGA GCCAAGTGCA ACAACACAAC AGCAGCATCA GCAGCAGGAT 
ACAGAGGATC TTGAGGAGAA CAAGGATGCG GGGGAGGCTA GTTTAAATGT TAGCAATAAT 
CACAATACAA CCGATAGCAA TAATAGTTGT AGTCGAAAGA ACAACAATGG CGGCAATGAA 
AGTGAGCAAC ATGTGGCCAG TTCGGCGGAG GACGATGATT GCGCCAACAA CAATACAAAT 
ACCAGCAATA ACAACAATAC CAGCAACACC GCCACCAGCA ACACGAACAA CAACAATAAT 
AATAACAGCA GCAGCGGCAA CAGCGAGAAG CGCAAGAAGA AGAACAATAA CAACAACAAC 
GGCCAGCCTG CTGTTCTATT AGCTGCCAAA GATAAAGAGA TTAAAGCCCT TTTGGACGAA 
CTGCAGCGCT TGAGGGCGCA GGAGCAGACG CACCTGGTGC AAATCCAACG ATTGGAGGAA 
CATCTTGAGG TCAAGCGTCA GCACATCATC CGATTGGAGG CACGTCTGGA TAAGCAGCAA 
ATTAATGAGG CACTGGCCGA GGCGACCGCT TTGTCCGCTG CCGCGTCAAC CAACAACAAC 
AATAATAGCC AGAGCAGCGA CAACAATAAG AAGCTGAATA CCGCTGCAGA AAGACCGATG 
GATGCGTCAT CCAATGCCGA TCTACCGGAG TCCACAAAAG CTCCAGTTCC GGCGGAGGAT 
GACGAAGAAG ATGAGGATCA GGCCATGCTG GTGGATTCCG AGGAAGCGGA GGACAAGCCA 
GAGGACTCAC ATCACGATGA CGACGAAGAT GAGGACGAGG ATCGGGAGGC GGTCAATGCC 
ACCACAACCG ATAGCAACGA GCTCAAAATC AAAAAGGAAC AGCACAGTCC GCTGGATCTG 
AATGTGCTCA GTCCGAATTC GGCCATTGCC GCTGCAGCTG CGGCCGCCGC TGCCGCCGCC 
TGCGCCAATG ACCCCAACAA ATTCCAGGCT CTGCTCATCG AGCGGACCAA AGCCTTGGCC 
GCAGAGGCGC TCAAGAATGG CGCCAGCGAT GCCCTCAGCG AAGAAACCAA CCGAAATCAT 
GAAGACGAAG ACGAAGTGTC GCCAGCAGCA ACTGCAACTG CAACACTCGC AACGCCTGCA 
ACAGTGGCAA CACCAATTGA AACACTGGCC ACCGATTCGG AAGCAAAAGC CAGCAATGAC 
ATGGCGCCCA AGCAGGAAAT CGAAGAAGTG GATGACGAGG ATGCCACCGA TGAACCGGTG 
ATCCTGCCGG AGCAACTAGT CAACGACTAT TGGCGACGGG GCTTTGTCGA ATCGGGGAAT 
AGCACTAATA CGAGCACCGC TGCAATGCCA GGGAGCAAAA CGCCAACAAT TTATCAAAAT 
TTTAAGGTCG AGCAGCAGGC ACAGTTGCTG CCGCCACCGC CGCCGCCACC ACCACCCCCG 
CAGCAACAAC ATCAACAGCA GCAACAACAA CAACATCTAC ACTTTTCACA GCAACAGCAA 
CAACAACATC AGCAGCAACA TGGACCATCG TCCACAGCCC TGCTTAGTCA ACTGGTTAAC 
TCAACATTTT CCAACTCTTC CAACTCTTCT CGCCTAGATG CCCACCATCA GCAGCAGCAG 
CACCACCAAC AGCAGCACCA ACACCAGCAA CAACACCACC AGCAACAGCA CCTCCACCAG 
CAACATCACC ATCACCTGCA ACAGCAACCA AACAGCGGCA GCAACAGCAA TCCAGCGAGC 
AACGACCACC ATCATGGACA CCATTTGCAT GGCCATGGTT TGCTGCATCC TTCGTCGGCG 
CACCATCTGC ACCACCAAAC GACCGAATCC AATTCGAATT CGAGTACACC CACAGCGGCG 
GGAAATAACA ATGGTAGCAA TAACAGCTCC AGCAACACAA ATGCCAATAG TACCGCTCAA 
TTGGCCGCCA GTTTGGCCAG TACACTCAAT GGCACCAAGT CCCTGATGCA GGAGGATAGC 
AATGGCTTGG CCGCCGTCGC CATGGCGGCT CATGCCCAAC ATGCGGCTGC CTTGGGACCT 
GGATTTTTGC CCGGCCTTCC AGCGTTTCAG TTTGCCGCCG CCCAAGTGGC CGCCGGTGGC 
GATGGACGGG GTCATTATCG TTTCGCCGAC TCGGAACTGC AATTGCCACC GGGTGCTTCG 
ATGGCCGGTC GTCTTGGAGA GTCGCTCATA CCCAAGGGTG ATCCCATGGA GGCCAAACTC 
CAAGAGATGT TGCGCTACAA CATGGACAAG TACGCCAATC AGGCGCTGGA TACCCTGCAC 
ATTTCGCGAC GTGTCCGCGA ACTGCTATCG GTGCACAACA TTGGCCAGCG ACTATTTGCC 
AAATACATAC TGGGCCTGTC GCAGGGCACC GTTTCCGAGC TGCTGAGCAA ACCCAAGCCG 
TGGGACAAGT TGACGGAGAA GGGACGAGAT AGCTACCGCA AAATGCACGC CTGGGCCTGT 
GACGATAATG CCGTCATGCT GCTCAAATCG CTGATACCCA AGAAAGATTC TGGACTGCCG 
CAGTATGCGG GTCGTGGTGC CGGCGGCGCT GGCGGCGATG ATTCCATGTC CGAGGATCGG 
ATTGCACATA TCCTCAGCGA AGCCTCCTCG CTGATGAAAC AGAGCAGTGT GGCCCAGCAT 
CGGGAGCAGG AGCGTCGCAG TCACGGCGGC GAGGATTCAC ACAGCAACGA GGACAGCAAA 
TCGCCACCTC AAAGCTGCAC GTCGCCGTTC TTCAAGGTGG AGAATCAGCT GAAGCAGCAC 
CAGCATCTTA ATCCCGAGCA GGCTGCCGCT CAGCAAAGGG AAAGGGAACG CGAGCAAAGA 
GAACGGGAGC AGCAGCAGCG GCTGCGGCAC GACGACCAGG ACAAGATGGC TCGTCTCTAC 
CAGGAGCTGA TTGCACGCAC TCCGCGTGAG ACGGCTTTTC CAAGCTTTCT CTTCTCACCC 
TCGCTATTTG GAGGAGCCGC CGGAATGCCA GGAGCAGCCA GCAATGCCTT TCCCGCCATG 
GCCGATGAAA ATATGCGTCA CGTCTTTGAA CGTGAGATTG CCAAGCTGCA GCAGCACCAA 
CAGCAACAAC AAGCTGCCCA GGCTCAGGCA CAGTTCCCCA ACTTCTCGAG CCTGATGGCT 
TTGCAGCAGC AGGTCCTCAA CGGAGCCCAG GATCTCTCGC TAGCTGCCGC TGCAGCCAAG 
GACATCAAGT TGAACGGTCA GCGATCCTCG TTGGAGCACA GTGCCGGCAG CAGCAGTTGT 
TCAAAAGATG GCGAACGAGA TGATGCGTAT CCAAGTTCCC TACATGGTCG CAAGTCCGAG 
GGAGGTGGCA CACCCGCACC ACCTGCCCCA CCATCTGGCC CAGGAACCGG AGCAGGAGCC 
CCACCCACAG CAGCACCACC AACCGGCGGT GCCAGCAGCA ATTCGGCAGC ACCCAGTCCG 
CTAAGCAATT CAATACTTCC ACCGGCATTG AGCAGCCAAG GTGAGGAGTT CGCCGCGACA 
GCAAGTCCTT TGCAGCGAAT GGCCTCGATC ACCAATTCGC TGATTACCCA ACCACCGGTG 
ACGCCGCACC ACAGTACGCC ACAGCGACCC ACCAAGGCGG TTCTGCCACC GATCACCCAG 
CAACAGTTCG ATATGTTCAA CAACCTTAAC ACCGAGGATA TAGTCAGACG TGTGAAGGAG 
GCCCTGTCGC AGTACTCCAT CTCCCAGCGA TTGTTCGGCG AGTCCGTGCT GGGTTTGTCT 
CAGGGATCGG TCTCCGATTT ATTGGCTAGA CCCAAGCCAT GGCACATGCT CACTCAAAAG 
GGCCGTGAAC CCTTCATTCG CATGAAGATG TTCCTGGAGG ATGAGAACGC GGTGCACAAA 
CTGGTGGCCA GTCAATACAA GATTGCCCCC GAGAAGCTAA TGCGAACGGG CAGTTACAGT 
GGAAGTCCGC AGATGCCACA GGGATTGGCA AGCAAAATGC AGGCTGCCTC CCTGCCCATG 
CAGAAGATGA TGAGCGAACT TAAGCTGCAG GAGCCAGCAC AGGCGCAGCA CTTGATGCAG 
CAAATGCAGG CGGCGGCAAT GTCGGCAGCA ATGCAACAGC AGCAAGTGGC ACAAGCGCAG 
CAGCAGGCTC AGCAGGCGCA ACAGGCACAA CAGCATTTGC AGCAGCAGGC GCAGCAGCAC 
TTGCAACAGC AGCAACATCT TGCCCAGCAG CAACACCCCC ATCAGCAGCA CCATCAAGCG 
GCCGCCGCGG CTGCCGCATT GCACCACCAA AGCATGCTTC TGACCTCGCC CGGACTGCCA 
CCCCAGCATG CCATCAGTCT GCCACCTTCA GCGGGTGGAG CCCAACCAGG TGGTCCTGGT 
GGCAATCAGG GCAGCTCAAA TCCCTCAAAC AGCGAAAAGA AACCCATGCT GATGCCGGTT 
CATGGCACTA ATGCCATGAG AAGTCTGCAC CAGCACATGT CGCCCACCGT TTACGAAATG 
GCCGCTTTAA CCCAAGATCT GGACACCCAC GATATCACTA CCAAGATCAA GGAGGCGTTG 
TTGGCCAACA ATATTGGTCA GAAAATATTT GGCGAAGCCG TTTTGGGACT TTCGCAGGGA 
TCCGTATCTG AGCTGCTGAG CAAACCGAAG CCCTGGCACA TGCTCTCCAT CAAGGGCAGG 
GAACCCTTCA TCCGGATGCA ATTGTGGCTC AGTGATGCCA ACAATGTTGA ACGATTGCAG 
TTGCTGAAAA ATGAGCGACG GGAGGCCAGC AAACGAAGGA GGAGCACAGG CCCAAACCAA 
CAGGACAATA GTAGCGATAC CTCCAGCAAT GATACCAATG ACTTCTATAC CAGCAGTCCG 
GGTCCAGGAT CCGTCGGTTC GGGTGTCGGT GGTGCTCCGC CGAGCAAAAA ACAGCGTGTC 
CTCTTCTCCG AGGAGCAAAA GGAGGCACTT CGGCTGGCAT TCGCCTTGGA TCCGTATCCA 
AATGTGGGGA CCATCGAGTT TCTGGCCAAT GAGCTGGGCT TGGCAACGCG GACGATCACC 
AACTGGTTCC ACAATCATCG CATGCGATTG AAGCAACAGG TGCCACACGG ACCTGCCGGT 
CAAGATAATC CGATACCGAG TCGCGAGAGT ACCAGTGCCA CGCCCTTCGA TCCCGTCCAA 
TTCCGCATTC TGCTGCAGCA GCGTCTACTG GAGCTCCACA AGGAGCGAAT GGGCATGAGC 
GGTGCCCCCA TTCCCTATCC GCCGTACTTT GCTGCCGCTG CCATTTTGGG TCGCAGTCTG 
GCGGGGATTC CCGGTGCGGC AGCGGCCGCT GGAGCAGCTG CAGCAGCCGC CGCAGTTGGC 
GCCTCTGGTG GTGATGAGTT GCAGGCTCTG AATCAGGCCT TCAAGGAGCA GATGAGCGGT 
TTGGATCTGT CGATGCCCAC CTTAAAGCGG GAACGCAGCG ATGACTATCA GGACGATCTG 
GAACTGGAGG GTGGTGGTCA CAATCTAAGT GACAACGAAT CGCTGGAGGG TCAAGAGCCG 
GAGGACAAGA CCACCGATTA CGAGAAGGTG CTGCACAAAT CCGCTTTGGC CGCTGCCGCC 
GCTTACATGT CCAATGCAGT GCGGAGTTCA CGACGAAAGC CAGCTGCCCC ACAGTGGGTC 
AATCCCGCCG GAGCGGTGAC CAATCCAAGT GCTGTAGTTG CAGCCGTTGC TGCTGCCGCA 
GCTGCCGCGG CGGACAATGA ACGCATCATC AACGGGGTGT GCGTGATGCA GGCCTCCGAG 
TACGGCCGCG ATGATACTGA TAGCAACAAG CCAACGGACG GCGGCAACGA TTCCGATCAT 
GAGCACGCCC AGCTGGAGAT CGATCAGCGT TTCATGGAAC CGGAGGTGCA CATCAAACAG 
GAGGAGGACG ACGATGAGGA GCAATCGGGA TCGGTTAATC TGGACAACGA GGACAATGCG 
ACTAGCGAGC AAAAACTGAA GGTGATCAAC GAGGAGAAGC TGCGAATGGT GCGAGTTCGC 
CGGCTGAGCA GCACTGGTGG TGGTTCCTCT GAGGAAATGC CAGCTCCCCT CGCACCACCG 
CCTCCTCCGC CAGCAGCTTC TTCTTCTATC GTTAGTGGAG AAAGCACCAC CAGCAGCAGT 
AGTAGCAGCA ACACCAGCAG CAGCACACCG GCGGTAACCA CTGCAGCAGC AACTGCGGCA 
GCCGGTTGGA ACTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ct cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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