rg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rg
Entrez Gene ID	44531
Full Name	rugose
Synonyms	AKAP,Akap550,CG15464,CG15465/CG6775,CG15466,CG44835,CG6775,DAKAP550,Dmel\CG44835,Dmel_CG15464,Dmel_CG6775,fas
General protein information	Preferred Names rugose Names CG44835 gene product from transcript CG44835-RP A kinase anchor protein 550 A-kinase anchoring protein CG44835-PL CG44835-PM CG44835-PN CG44835-PO CG44835-PP CG44835-PQ CG44835-PR drosophila A kinase anchor protein facetious pebbled rg-PL rg-PM rg-PN rg-PO rg-PP rg-PQ rg-PR
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4F2-4F3\|1-11.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144685.2	NP_001138157.2	rugose, isoform O
NM_167028.3	NP_726978.2	rugose, isoform R
NM_001042796.3	NP_001036261.3	rugose, isoform L
NM_001144687.2	NP_001138159.2	rugose, isoform M
NM_001297949.1	NP_001284878.1	rugose, isoform P
NM_001297950.1	NP_001284879.1	rugose, isoform Q
NM_001144686.2	NP_001138158.2	rugose, isoform N

Pathways from BioSystems

Homologies

Homo sapiens (human)	NBEA	NP_056493.3
Gallus gallus (chicken)	NBEA	NP_001239069.1
Drosophila melanogaster (fruit fly)	rg	NP_001138157.1
Bos taurus (cattle)	NBEA	XP_005213801.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000017	XP_311142.5
Xenopus tropicalis (tropical clawed frog)	nbea	XP_002934066.2
	NBEA	XP_002808188.1
Canis lupus familiaris (dog)	NBEA	XP_005635521.1
Mus musculus (house mouse)	Nbea	NP_085098.1
Rattus norvegicus (Norway rat)	RGD1562629	XP_006232442.1
Danio rerio (zebrafish)	LOC565885	XP_001920727.1
Caenorhabditis elegans (roundworm)	sel-2	NP_497939.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

adult rg loss-of-function mutants exhibit defective social interactions, impaired habituation, aberrant locomotion, and hyperactivity.
Title: Drosophila mutants of the autism candidate gene neurobeachin (rugose) exhibit neuro-developmental disorders, aberrant synaptic properties, altered locomotion, and impaired adult social behavior and activity patterns.

This study demonistrated that functional homologies between Drosophila Rugose and mammalian NBEA that will help clarify the role of BEACH proteins in the development and function of the CNS in vivo.
Title: Drosophila rugose is a functional homolog of mammalian Neurobeachin and affects synaptic architecture, brain morphology, and associative learning.

Drosophila spoonbill encodes a dual-specificity A-kinase anchor protein essential for oogenesis.
Title: Drosophila spoonbill encodes a dual-specificity A-kinase anchor protein essential for oogenesis.

Rugose (rg) plays an important role in the integration of different signals required for the exact regulation of cone cell development and survival.
Title: Mutations in rugose promote cell type-specific apoptosis in the Drosophila eye.

results suggest that rugose gene encodes a secondary pigment cell specific function, and plays an essential role in determining the optic lobe's structure
Title: Mutation in rugose gene locus affects the pigment cell number and alters the optic lobe phenotype in the Drosophila visual system.

The following rg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa22214	NM_001297949.1 Latest version!	Drosophila melanogaster rugose (rg), transcript variant P, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22215	NM_001297950.1 Latest version!	Drosophila melanogaster rugose (rg), transcript variant Q, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22216	NM_001144686.2 Latest version!	Drosophila melanogaster rugose (rg), transcript variant N, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22217	NM_001144687.2 Latest version!	Drosophila melanogaster rugose (rg), transcript variant M, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22218	NM_001042796.3 Latest version!	Drosophila melanogaster rugose (rg), transcript variant L, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22219	NM_001144685.2 Latest version!	Drosophila melanogaster rugose (rg), transcript variant O, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22220	NM_167028.3 Latest version!	Drosophila melanogaster rugose (rg), transcript variant R, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rg ( NM_001297949.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284878.1 Drosophila melanogaster rugose (rg), transcript variant P, mRNA.

Price & Availability↑

CloneID	OFa22214
Clone ID Related Accession (Same CDS sequence)	NM_001297949.1
Accession Version	NM_001297949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform P
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCGC TGGAGCGTCT GATGCGAGCG GCCCCATTGC CACGAATGCT TACCAGTGGC 
GTTGTGGCCA CAGCCGCTGC CGCCGCATCC GCCGCCGCTG GCAAGGGCAT GGTCAGTGTG 
GGCGGTGGTG CAACAGCCAT AGCCGCCGGC GGTGGCCAAC AGAGGGCGCT GGTCCATGCC 
TCGCTGGCGG CGGCGACAGT GGGCCGCAAG GGACGCCACC TAACGGGCAC CTTCTGCCTA 
ACCGGCGACA CCATGGAGGG CATCATACAG TGTTTGGTAT TTCTCAAAGC ATTCTCGCTG 
GTCGGCGGTG AATTCGATAT GGAATTGAAT TTTGTGATAC AGGATGCGCA GAATATCAAA 
CATATGCTGG AGCTGCTGGA TCATTGTCCG CCCAATTTGC AGGCGGAGAT TTGGAGCGTA 
TTCATCGCCA TCCTGCGCAA AAGCGTCCGC AATCTGCAGG CCTGCACCGA TGTTGGCCTA 
ATCGAGCATG TTCTCGTCCG CCTGCAACGC TCCGAAACGG TTGTGGCAGA CCTGCTCATC 
GAGATGTTGG GCGTGCTGGC CAGCTATAGC ATAACGGTGA AGGAGCTAAA GCTCCTCTTC 
GGTACGATGA AGGCCACCAA TGGCAAGTGG CCGCGCCACT CGGCCAAGTT GCTCAACGTC 
CTCCGGCAGA TGCCACATCG CAACGGACCA GACGTCTTCT TCAGCTTTCC GGGTCGCAAG 
GGATCGGCCA TGGTACTGCC GCCACTGGCC AAATGGCCCT ATGAGAACGG ATTCACCTTT 
ACCACCTGGT TCCGCCTGGA TCCCATCAAT TCGGTGAATA TTGAACGTGA GAAGCCCTAC 
CTCTACTGCT TCAAGACATC GAAGGGCGTG GGCTATACGG CGCATTTTGT GGGAAATTGC 
CTTGTCCTCA CCTCAATGAA GGTCAAGGGC AAAGGCTTTC AGCATTGTGT CAAATACGAA 
TTCCAGCCAC GAAAGTGGTA TATGATTGCC ATAGTGTATA TATACAATCG TTGGACGAAA 
AGCGAAATCA AGTGCCTCGT TAATGGACAG CTGGCCTCTT CAACGGAGAT GGCCTGGTTT 
GTTTCCACAA ACGATCCCTT TGACAAGTGC TACATTGGAG CCACCCCCGA GTTGGACGAG 
GAGCGCGTGT TCTGTGGCCA AATGTCGGCG ATCTACCTTT TCAGCGAGGC ACTGACCACG 
CAGCAGATCT GTGCGATGCA TCGCCTGGGT CCCGGCTACA AGTCCCAGTT CCGTTTCGAC 
AACGAGTGCT ATCTGAATCT GCCGGACAAT CACAAGCGGG TGAGTCACTT TCAACTTCTG 
CCAGCGACCT TGGGGGCGTC AGCACTGTCG GGCGGCAGTG GGAGTGGCAC CGGAAGCGGT 
ACCGGCAACG ATGCATCAGC TGCAGCGGCA GCGGCTGTTG CCGCCGGTCA GCAACAGCAG 
TTGCAGCTGC AGTTCCAAAT TCTGGCCGCC GAGCAAGAGG CGCGTGCCAT CGATTGGTCC 
GATGAGAAGC TCGATTTGAA TGCGGCTTTT GTGAAAATTC GAGCGGTTCT CACCGCCCGC 
AATGCAGTGA CCTTGGCTGG CAGCAGCAGC ACTGGCACCA CTGCAGTAGC CACTGCCGCA 
GCAGCAGCAG CAGCAGCGGG AGCGGGAGCA GGAACAACTG CTGCAGCAAC ATCTGCAGCA 
GCAGCGGCGG CAGCAACACA AAATGAGAAT GATGCAGCAG TGGGACAGCA GCAACATGCA 
ACACATCACC ATGCCACAGC AGCAACTGGC AGTGCGGACG ATCCGCTTGG CCATTTGCCC 
ACTGGAAATG CTTCTTCTTC TTCATCTTCT TTTGAGCAAC TACGTCGAAT GTCCAGTGTG 
AGCAGCTTGA ACTCAATGGT CGGAAGCGCC GACACTGAGG AGGTCAACCA GCTGAAAGCT 
GTGCTGTACG ATGGCAAACT ATCGAATGCC ATTGTGTTCA TGTACAATCC GGTGGCCACC 
GATGGTCAGT TGTGTCTGCA GTCATCGCCC AAGGGAAACG TTTCATATTT CGTGCACACG 
CCGCATGCGC TGATGTTGCA GGATGTGAAG GCCGTGGTCA CGCACTCGAT ACACTGCACC 
CTCAATTCGA TTGGCGGCAT CCAGGTGCTA TTCCCGCTTT TCTCGCAGCT GGATATGGCC 
CACGAGGGTC TGGGCGACAT TAAGCGGGAT CCCACGCTGT GCTCCAAGCT GCTGGGCTTC 
ATCTGTGAAC TGGTGGAAAC ATCGCAGACG GTGCAGCAGC ATATGATCCA GAACCGGGGA 
TTCCTGGTCA TCTCGTTCAT GCTGCAGCGC TCCTCCCGCG AACACCTGAC TCTCGAAGTC 
CTGGGATCAT TCCTGAATCT GACCAAGTAT CTGGTCACCT GCCTGTCGGC CAACAGTGAT 
CTGCTGCTCA AACAGCTGCT GGATCACGTC CTCTTCAATC CAGCCCTGTG GATATACACA 
CCCGCCAATG TCCAGGCGCG ACTATACTCC TACTTGGCCA CCGAGTTCCT CTCGGACACG 
CAGATCTACA GCAATGTGAG GAGGGTCAGC ACGGTACTGC AGACAGTGCA CACCCTAAAG 
TACTACTACT GGGTGGTCAA TCCACGGGCT AAGAGCGGCA TCATTCCCAA GGGACTGGAT 
GGACCTCGTC CGGCTCAAAA GGACATCCTG GCCATCCGGG CATACATCCT GCTGTTCCTG 
AAGCAGCTCA TAATGATCGG CAATGGCGTG AAGGAGGACG AGCTGCAGAG CATACTCAAC 
TATCTGACCA CCATGCACGA GGACGAGAAC CTGCACGACG TGCTGCAGAT GCTCATCTCG 
CTGATGTCGG AGCATCCCAG CTCCATGGTA CCTGCCTTTG ATGTGAAGCA CGGCGTGCGC 
AGCATCTTCA AGTTGCTGGC GGCCGAAAGT CAGTTGATTC GGCTTCAGGC CCTCAAGCTG 
CTGGGCTTCT TCCTTTCCCG CAGCACCCAC AAACGCAAGT ACGATGTGAT GTCACCACAC 
AATCTGTACA CCCTTTTAGC GGAGCGACTG CTTCTCTATG AGGAATCACT GTCCCTGCCC 
ACATACAATG TTCTCTATGA GATCATGACG GAGCACATCT CGCAGCAGAT TCTATACACA 
CGCCATCCGG AACCAGAGAG TCACTACCGT CTGGAGAATC CAATGATCCT CAAGGTGGTG 
GCCACCCTGA TCCGGCAATC AAAGCAGACG GAATCGCTGA TCGACGTTAA AAAGCTATTC 
CTGCAGGATA TGACCCTGTT GTGCAATAGC AATCGGGAGA ACCGGCGCAC AGTGCTCCAG 
ATGTCCGTGT GGCAGGAGTG GCTCATTGCG ATGGCGTACA TTCATCCAAA GAGCAGCGAG 
GAGCAGAAGA TAAGCGACAT GGTGTATTCC CTATTCCGCA TGCTGCTCCA CCATGCCATT 
AAGCATGAAT ACGGTGGCTG GAGAGTTTGG GTAGATACCC TGGCAATCGT CCACTCCAAG 
GTGTCTTACG AGGAGTTTAA GCTCCAGTTT GCCCAGATGT ACGAGCACTA CGAGCGCCAG 
CGAACGGACA ATATCACAGA TCCTGCCCTG CGTCAGGCTA GACCCATCAG CACGATCAGT 
GGTTGGGAGC GAGAGGAGCT GCATCAGCAG CAGAACGGTG GATCTGCAGC AGCAGTGGCT 
ACCAATCAAA CGGCAGCTGT TAAAGGTTCT GTTTCCATTG CCTCACTGGA AGATGTGCCA 
CCGGTGGTTG AGGAAGAGGT GGAGGAACTG GAGCTGGAGG AAGTAGAGAT TCAAGAGGGT 
CCTATAACCG AAGAAACCGA ACAAAAATCG GTGATAGCCA ACATTTCCGA CGTCTACAAT 
GAGCAGCTCA AAACCGATGC CACGTGCAAT GGCAATTTGG AGGATGTCAA AGAGGAGGAG 
CCTGTACAAC AGCAAATCGG AGACCTGGAG AAGCAACCAG AGCCATCTAC TCCTTTGGGA 
GCACTCAGGG AAACCCTACA ACTTGGTGAT GATATGGATG TGGAAGAACT GGAGCTGGCT 
ACCGCCAAGG ATGCCCTCAA TGCGGAACAG CATGTGTCGC GAGTTCTTCA GGCTTCCGAA 
GCGGCACTTA ACGATTGCAA AATGGCAGTC GATGATGTCC TGCAGGAGTC ATCCTCCGTC 
CTCAAGGACG AGGAGATCGA ACTGGCCGTC AACGAGGTTG TTCAGGGTGT GCTCAATAAC 
GAGAAGAAAA CTCAATCCCA GGACAACAAG GATAACAAGG AACAGCCGGG CGAACAGGAT 
GTAAACGTCA GTCTCCTAAA CAGCAAGAAT CTGCTCAACA ATAATAATAA TAACAATAAC 
AACAGTCCGA GTCCAACGCC CACAACGGCG ACTGCGACGG CGGAGACGGA AGCGGAAACA 
GAAGTCAATG CCAATGAGAT CGTGAGCAGC ACAGAGGCGC CCAAGGCGGA AACGGAAACG 
AGCGTAGCAC CGGAAGTAGA AACACCGGAA ACGGCCAAGC CAAGCCCCAT TGTCCCCAGC 
CCCGTATTAG CAACCAATCA AAAGACTGAA GATGCAGCCA ACAAGCTGAA CAACAACGAG 
AAGCTGGCCG AGATCAGCGC CAGTCCCGAG CCACCCATTG TCGTGGAAAC GCCAGAAGCT 
GATCTTCTTC AGCTTTCCGA TTCCGAAACA AAACCGAATA AGGAAACCGA AGCTGAAGAT 
TCGGTGGCGC TGGCCGTTAG AGATATTGTC GAGCAACTCA TCGACAAGGT CATCGATGCC 
ACGGAAGCGG AATCGGCCAG CGAAACCAAA ACGGAAACGA ATAATAATGA AATACCCAAG 
AAGGAGAAGC AGACGTCAGA AGAGCCTGAG GATGTGGAAA CTGCAGAGAC TTTGGCCGCT 
GCCGCCAAGG AAATTGTCCA AGAGGTTGTG GAAGCAGCTC TCGTAATGGT CCAAGAGGAG 
AGCACTCAAG AGAAGCCAGA AAAGGGGGCG AACTCCGAAG AGGAGAAAAA CGAAATTGGC 
AAGGAAGAGA TTCTTCTGCA GCTGGAGGAA AAACCAGCGT CTACTGAAGT GCAGGAAACC 
AAGATCGAAG GAGATCTAAA GAAACCAGAA GATCCAAAAG GTCACAGCTC TGTAGAACCA 
AAGACCCCGA ACCTTGAGGA ACCGAAACCC CAGGAAACTG AACAACAAAA GTCGCAGGAG 
GTCGCCGAAG AACTACCTCA AAAACCAGAG GAGCAGGTGG TGGCCATTGT CAACCAAGTC 
CTGGACACTC TGGTAGACGA TACGGTCAAG GCAGTGGCAG CCGAGCAAAC CACTCAAACA 
TCGCCAGCCC CCGAAGAGCA ATCGCCACAA ATACTGGCAA TGGAATCACC AGCAACGTCG 
GTGCGGGTCA AACCCACTGA GGTGGATTCC ACCACCCAGA CTACGCCGAA GAACGAGGCT 
GGATCCAGTC TATTGGTTGA GCAGGTGCAG CAAGTCCTCC AGGAGGATGA CGCCCAGCAA 
TCAGCCGGCA TGACCATCGA AGATGAGGAT TACTCTAATC AGCAGGCAGC TGCGGCAGTT 
GAAAATGCCA ACAGCAGCCA ATTGGATGCC AATCATTATG GTCCCGGTAA TCCAGAATCC 
AAACAACAGC AACAGCGCTC CAAATCGGGA TCGACCCGAC CTATGTTCAG TCCGGGACCT 
ACACGTCCGC CCTTCCGAAT ACCGGAATTC AAGTGGTCCT ACATCCATCA GCGATTGCTC 
AGCGATGTCC TCTTTTCGCT GGAAACGGAC ATTCAGGTGT GGCGCAGTCA TTCGACCAAA 
AGCGTCCTGG ACTTTGTCAA CTCCAGCGAG AATGCAATTT TTGTGGTGAA CACGGTGCAT 
TTAATATCGC AGCTAGCGGA TAATCTGATT ATTGCCTGCG GAGGATTGCT GCCTCTTTTG 
GCCAGCGCCA CGTCACCCAA TTCCGAATTG GATGTGCTGG AGCCAACGCA AGGCATGCCT 
TTGGAGGTGG CCGTATCTTT CCTGCAGCGT CTGGTCAACA TGGCGGATGT CCTGATCTTT 
GCCACATCCC TAAACTTCGG CGAGTTGGAG GCGGAGAAGA ACATGTCCAG TGGCGGCATA 
CTGCGTCAGT GCCTGCGATT GGTTTGCACC TGTGCGGTAA GGAATTGCTT GGAGTGCAAG 
GAGCGAACGC GTTACAATGT GGGCGCCTTG GCGAGAGATG TCCCGGGTGC AGCCCATTTG 
CAGGCCCTCA TCCGCGGAGC TCAAGCATCG CCCAAGAACA TTGTCGAGTC AATAACCGGC 
CAATTATCGC CTGTCAAGGA TCCCGAGAAG CTGCTCCAGG ACATGGACGT CAATCGCCTG 
CGCGCAGTCA TCTATCGCGA TGTGGAGGAG ACTAAGCAGG CGCAGTTCCT CTCGCTGGCG 
ATAGTGTACT TCATATCGGT GCTGATGGTG TCCAAGTATC GTGATATCCT GGAGCCGCCG 
GCAGAGCCGC AAATCCAGCG TCAATCGCCA GTGCTGCAAC GCACGGCAGG CGGCGGTGGT 
CGTCAAATCC AGGATAGTGA CTACGAAATA ATTGTCGTCG ATGAGAACAA TCCATCGGTT 
TTGGCGGATA ATGATTCACA TTCCAGTGGA CCACCATCCA TAAAGAGCGT TGACTCGGAT 
GTGGGCTCCC TGAACATGAA CTCCACGGAA AACGAAGTGC CCGAGGTGGA GTCATCCAGC 
GAGATTCTAA TCGATGACCA CAAACCGAGT CACTCGAACG ACGAAAGCTG GACGGATGTG 
AATCTCAATG AGGATGCTGC CGTTCAGGCG GCAAGTGCCG GAATAGTTGT AGGTTTGGTG 
GATAACAGAG GTAATGTTAT ATCCGACAAA CACGATCCGT CCCATCACAA CCAACAACAG 
CAGCAGCAGG CGGGAATTAT TGGTCAGCAG CAGCAACATG GATCACTTGG TCATTCGGAG 
CGGGGTGACA AACCCGATTC GGAGATTTCG GTGGTGCGAG TGCCTGATGG TTATGGTGGT 
GCTGGTTCCG GTGGTGGCGT GAATTCCGGA CAAGGTCAGG GCGTTCCATC CAATCAGCGT 
CCTCGTCCCG AGGAGTTGCC CATGAAGGCG CCCGCCTTGG TGGCCCAACT GCCGCTGACC 
ACACCTTCGC GAGAGGCCAG TCTCACCCAG AAGCTGGAAA TCGCATTGGG GCCAGTGTGT 
CCGCTGCTCC GTGAAATCAT GGTGGACTTT GCGCCGTTCC TTTCGAAAAC CCTAGTCGGC 
TCCCATGGCC AGGAATTGCT GATGGAGGGC AAGGGTCTGA CGACCTTTAA GAACTCGCAT 
TCCGTCGTAG AGCTGGTCAT GTTGCTCTGC TCCCAGGAGT GGCAAAATAG CCTACAAAAG 
CACGCAGGAT TAGCCTTTAT TGAGCTAATC AATGAGGGTC GACTGCTTTC GCACGCCATG 
AAGGATCACA TTGTTCGGGT GGCCAATGAG GCGGAGTTCA TCCTCAATAG AATGCGAGCG 
GATGATGTCC TCAAGCATGC CGACTTTGAA TCGCAATGTG CACAGACACT TTTGGAGCGC 
AGGGAGGAGG AGCGAATGTG CGATCACTTG ATAACCGCCG CACGTCGCAG AGACAATGTG 
ATTGCCAGCC GACTGCTGGA GAAGGTACGA AACATCATGT GTAATCGCCA CGGAGCCTGG 
GGTGATTCCA GCTCCACATC CAGTGGTGGC GCCATCGTTG GAGCGGTGCA AAAGAGTCCC 
TACTGGAAGC TGGATGCCTG GGAGGATGAT GCCAGACGTC GGAAACGGAT GGTGCAAAAT 
CCGCGCGGAT CCTCGCATCC ACAGGCCACA CTGAAGGCGG CTTTGGAAAA TGGCGGACCT 
GAAGATGCCA TCCTGCAGAC TCGCGACGAG TTCCACACCC AAATAGCCGT CTCGCGGACT 
CATCCATCCG GTCAGCACAA TGGTGAGCTA CTGGACGACG CCGAGCTCTT GATTGAGGAT 
CGGGAGTTGG ATCTGGATCT CACGGGTCCC GTCAACATTA GCACCAAGGC GAGATTGATA 
GCCCCCGGTC TGGTGGCACC CGGCACCGTC TCAATAACCA GCACTGAAAT GTTCTTTGAG 
GTGGATGAAG AGCATCCCGA GTTCCAGAAG ATCGATGGCG AAGTTCTTAA GTACTGTGAC 
CATTTGCACG GCAAATGGTA CTTCTCCGAA GTGAGGGCCA TCTTCTCGAG GCGTTATCTC 
CTGCAAAATG TGGCCCTCGA GATCTTTTTG GCCAGCCGTA CGTCCATATT GTTTGCCTTT 
CCCGATCAGC ATACGGTCAA GAAGGTAATC AAGGCCCTGC CCCGTGTGGG AGTGGGCATC 
AAGTATGGAA TACCGCAGAC GAGGAGGGCC TCAATGATGT CGCCCCGTCA ACTGATGCGC 
AATTCGAACA TGACCCAAAA GTGGCAGCGT CGCGAGATTA GCAACTTTGA GTATCTTATG 
TTCCTGAACA CGATCGCCGG CAGGACGTAC AACGATCTAA ATCAGTATCC CATCTTTCCG 
TGGGTGCTGA CCAACTATGA GTCCAAGGAT TTGGATCTCA GCCTGCCGTC AAACTACAGG 
GATCTGTCGA AACCGATTGG CGCCCTGAAT CCATCGCGCA GAGCCTATTT CGAGGAGCGT 
TACGAGAGCT GGGACAGTGA CACCATACCG CCCTTCCACT ATGGTACGCA TTATTCCACG 
GCAGCATTTA CGCTGAACTG GTTGGTGCGC GTGGAACCGT TTACCACCAT GTTCCTGGCC 
CTGCAGGGCG GCAAATTTGA TTATCCGGAC AGGCTGTTCA GCTCGGTGTC GCTGTCCTGG 
AAGAATTGCC AACGTGATAC ATCGGATGTG AAGGAACTGA TACCAGAATG GTATTTCCTG 
CCCGAAATGT TTTACAATTC ATCAGGCTAT CGACTGGGTC ATCGCGAGGA TGGTGCCCTA 
GTGGATGACA TTGAACTACC ACCGTGGGCC AAGAGCCCCG AAGAATTCGT CCGCATCAAT 
CGCATGGCAC TGGAGTCGGA GTTCGTGTCC TGCCAGCTGC ACCAGTGGAT CGATTTGATC 
TTTGGCTACA AGCAACGCGG TCCCGAGGCC ATTAGGGCAA CCAACGTGTT CTACTATTTG 
ACATATGAGG GCAGCGTTGA CTTGGATGGC GTTTTGGATC CGGTGATGCG CGAAGCCGTC 
GAGAATCAAA TCCGCAACTT TGGCCAAACA CCTAGTCAAC TGCTAATGGA ACCGCATCCG 
CCGCGCAGCT CTGCGATGCA TCTATCGCCT ATGATGTTCA GTGCGATGCC GGAGGATCTC 
TGCCAGATGC TCAAGTTCTA TCAGAACTCA CCAGTCATTC ACATCTCGGC CAACACCTAT 
CCGCAACTGT CGTTGCCATC TGTGGTCACG GTAACGGCGG GTCATCAGTT TGCGGTGAAT 
CGATGGAACT GCAACTACAC CGCCTCCGTC CAGAGTCCCA GCTACGCCGA ATCGCCCCAA 
TCGCCGGGTT CCAATCAACC ACTGACCATC GATCCGGTTC TGGCTGTTCA TGGCACCAAC 
AACAATAGCA ATGCGGCCAG CCGACGTCAT TTGGGCGATA ACTTTAGCCA GATGCTAAAG 
ATACGCTCCA ACTGCTTTGT TACCACAGTG GACAGTCGGT TCCTAATCGC ATGCGGATTC 
TGGGACAACA GTTTCCGCGT CTTTGCCACC GAAACGGCGA AAATCGTGCA GATTGTATTC 
GGTCACTTTG GTGTGGTAAC GTGCATGGCA CGTTCCGAGT GCAACATCAC ATCAGACTGC 
TACATCGCCT CCGGATCGGC GGACTGCACG GTGCTTCTGT GGCACTGGAA TGCCCGAACC 
CAGAGCATTG TGGGCGAGGG CGATGTGCCC ACACCACGGG CCACCTTGAC GGGTCACGAA 
CAGGCCGTGA CATCGGTGGT GATTAGTGCC GAGTTGGGTC TGGTTGTGTC TGGATCATCA 
AATGGACCCG TACTTATCCA TACGACTTTT GGCGACCTGC TGCGCTCACT GGATCCACCA 
GCGGAGTTCC ATTCCCCGGA ACTGATAACC ATGTCCCGCG AGGGTTTCAT TGTCATCAAC 
TACGATAAGG GCAATGTGGC CGCCTACACC ATCAATGGCA AGAAACTGCG CCACGAGACG 
CACAACGACA ATCTACAGTG CATGCTACTG TCGCGCGATG GTGAATACCT GATGACCGCC 
GGTGATCGCG GCATCGTGGA GGTGTGGCGC ACCTTCAACC TAGCACCACT TTATGCCTTC 
CCCGCATGCA ACGCGGGCAT CCGATCTTTG GCTCTCACCC ACGATCAGAA ATACCTTCTG 
GCCGGACTTT CGACGGGCTC GATCATAGTA TTCCACATCG ACTTCAATCG CTGGCACCAC 
GAGTACCAGC AGCGCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284878.1
CDS	4968..15308
Translation	MDSLERLMRAAPLPRMLTSGVVATAAAAASAAAGKGMVSVGGGATAIAAGGGQQRALVHASLAAATVGRKGRHLTGTFCLTGDTMEGIIQCLVFLKAFSLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSHFQLLPATLGASALSGGSGSGTGSGTGNDASAAAAAAVAAGQQQQLQLQFQILAAEQEARAIDWSDEKLDLNAAFVKIRAVLTARNAVTLAGSSSTGTTAVATAAAAAAAAGAGAGTTAAATSAAAAAAATQNENDAAVGQQQHATHHHATAATGSADDPLGHLPTGNASSSSSSFEQLRRMSSVSSLNSMVGSADTEEVNQLKAVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_001297949.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant P, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297949.1

ORF Insert Sequence:

ATGGACTCGC TGGAGCGTCT GATGCGAGCG GCCCCATTGC CACGAATGCT TACCAGTGGC 
GTTGTGGCCA CAGCCGCTGC CGCCGCATCC GCCGCCGCTG GCAAGGGCAT GGTCAGTGTG 
GGCGGTGGTG CAACAGCCAT AGCCGCCGGC GGTGGCCAAC AGAGGGCGCT GGTCCATGCC 
TCGCTGGCGG CGGCGACAGT GGGCCGCAAG GGACGCCACC TAACGGGCAC CTTCTGCCTA 
ACCGGCGACA CCATGGAGGG CATCATACAG TGTTTGGTAT TTCTCAAAGC ATTCTCGCTG 
GTCGGCGGTG AATTCGATAT GGAATTGAAT TTTGTGATAC AGGATGCGCA GAATATCAAA 
CATATGCTGG AGCTGCTGGA TCATTGTCCG CCCAATTTGC AGGCGGAGAT TTGGAGCGTA 
TTCATCGCCA TCCTGCGCAA AAGCGTCCGC AATCTGCAGG CCTGCACCGA TGTTGGCCTA 
ATCGAGCATG TTCTCGTCCG CCTGCAACGC TCCGAAACGG TTGTGGCAGA CCTGCTCATC 
GAGATGTTGG GCGTGCTGGC CAGCTATAGC ATAACGGTGA AGGAGCTAAA GCTCCTCTTC 
GGTACGATGA AGGCCACCAA TGGCAAGTGG CCGCGCCACT CGGCCAAGTT GCTCAACGTC 
CTCCGGCAGA TGCCACATCG CAACGGACCA GACGTCTTCT TCAGCTTTCC GGGTCGCAAG 
GGATCGGCCA TGGTACTGCC GCCACTGGCC AAATGGCCCT ATGAGAACGG ATTCACCTTT 
ACCACCTGGT TCCGCCTGGA TCCCATCAAT TCGGTGAATA TTGAACGTGA GAAGCCCTAC 
CTCTACTGCT TCAAGACATC GAAGGGCGTG GGCTATACGG CGCATTTTGT GGGAAATTGC 
CTTGTCCTCA CCTCAATGAA GGTCAAGGGC AAAGGCTTTC AGCATTGTGT CAAATACGAA 
TTCCAGCCAC GAAAGTGGTA TATGATTGCC ATAGTGTATA TATACAATCG TTGGACGAAA 
AGCGAAATCA AGTGCCTCGT TAATGGACAG CTGGCCTCTT CAACGGAGAT GGCCTGGTTT 
GTTTCCACAA ACGATCCCTT TGACAAGTGC TACATTGGAG CCACCCCCGA GTTGGACGAG 
GAGCGCGTGT TCTGTGGCCA AATGTCGGCG ATCTACCTTT TCAGCGAGGC ACTGACCACG 
CAGCAGATCT GTGCGATGCA TCGCCTGGGT CCCGGCTACA AGTCCCAGTT CCGTTTCGAC 
AACGAGTGCT ATCTGAATCT GCCGGACAAT CACAAGCGGG TGAGTCACTT TCAACTTCTG 
CCAGCGACCT TGGGGGCGTC AGCACTGTCG GGCGGCAGTG GGAGTGGCAC CGGAAGCGGT 
ACCGGCAACG ATGCATCAGC TGCAGCGGCA GCGGCTGTTG CCGCCGGTCA GCAACAGCAG 
TTGCAGCTGC AGTTCCAAAT TCTGGCCGCC GAGCAAGAGG CGCGTGCCAT CGATTGGTCC 
GATGAGAAGC TCGATTTGAA TGCGGCTTTT GTGAAAATTC GAGCGGTTCT CACCGCCCGC 
AATGCAGTGA CCTTGGCTGG CAGCAGCAGC ACTGGCACCA CTGCAGTAGC CACTGCCGCA 
GCAGCAGCAG CAGCAGCGGG AGCGGGAGCA GGAACAACTG CTGCAGCAAC ATCTGCAGCA 
GCAGCGGCGG CAGCAACACA AAATGAGAAT GATGCAGCAG TGGGACAGCA GCAACATGCA 
ACACATCACC ATGCCACAGC AGCAACTGGC AGTGCGGACG ATCCGCTTGG CCATTTGCCC 
ACTGGAAATG CTTCTTCTTC TTCATCTTCT TTTGAGCAAC TACGTCGAAT GTCCAGTGTG 
AGCAGCTTGA ACTCAATGGT CGGAAGCGCC GACACTGAGG AGGTCAACCA GCTGAAAGCT 
GTGCTGTACG ATGGCAAACT ATCGAATGCC ATTGTGTTCA TGTACAATCC GGTGGCCACC 
GATGGTCAGT TGTGTCTGCA GTCATCGCCC AAGGGAAACG TTTCATATTT CGTGCACACG 
CCGCATGCGC TGATGTTGCA GGATGTGAAG GCCGTGGTCA CGCACTCGAT ACACTGCACC 
CTCAATTCGA TTGGCGGCAT CCAGGTGCTA TTCCCGCTTT TCTCGCAGCT GGATATGGCC 
CACGAGGGTC TGGGCGACAT TAAGCGGGAT CCCACGCTGT GCTCCAAGCT GCTGGGCTTC 
ATCTGTGAAC TGGTGGAAAC ATCGCAGACG GTGCAGCAGC ATATGATCCA GAACCGGGGA 
TTCCTGGTCA TCTCGTTCAT GCTGCAGCGC TCCTCCCGCG AACACCTGAC TCTCGAAGTC 
CTGGGATCAT TCCTGAATCT GACCAAGTAT CTGGTCACCT GCCTGTCGGC CAACAGTGAT 
CTGCTGCTCA AACAGCTGCT GGATCACGTC CTCTTCAATC CAGCCCTGTG GATATACACA 
CCCGCCAATG TCCAGGCGCG ACTATACTCC TACTTGGCCA CCGAGTTCCT CTCGGACACG 
CAGATCTACA GCAATGTGAG GAGGGTCAGC ACGGTACTGC AGACAGTGCA CACCCTAAAG 
TACTACTACT GGGTGGTCAA TCCACGGGCT AAGAGCGGCA TCATTCCCAA GGGACTGGAT 
GGACCTCGTC CGGCTCAAAA GGACATCCTG GCCATCCGGG CATACATCCT GCTGTTCCTG 
AAGCAGCTCA TAATGATCGG CAATGGCGTG AAGGAGGACG AGCTGCAGAG CATACTCAAC 
TATCTGACCA CCATGCACGA GGACGAGAAC CTGCACGACG TGCTGCAGAT GCTCATCTCG 
CTGATGTCGG AGCATCCCAG CTCCATGGTA CCTGCCTTTG ATGTGAAGCA CGGCGTGCGC 
AGCATCTTCA AGTTGCTGGC GGCCGAAAGT CAGTTGATTC GGCTTCAGGC CCTCAAGCTG 
CTGGGCTTCT TCCTTTCCCG CAGCACCCAC AAACGCAAGT ACGATGTGAT GTCACCACAC 
AATCTGTACA CCCTTTTAGC GGAGCGACTG CTTCTCTATG AGGAATCACT GTCCCTGCCC 
ACATACAATG TTCTCTATGA GATCATGACG GAGCACATCT CGCAGCAGAT TCTATACACA 
CGCCATCCGG AACCAGAGAG TCACTACCGT CTGGAGAATC CAATGATCCT CAAGGTGGTG 
GCCACCCTGA TCCGGCAATC AAAGCAGACG GAATCGCTGA TCGACGTTAA AAAGCTATTC 
CTGCAGGATA TGACCCTGTT GTGCAATAGC AATCGGGAGA ACCGGCGCAC AGTGCTCCAG 
ATGTCCGTGT GGCAGGAGTG GCTCATTGCG ATGGCGTACA TTCATCCAAA GAGCAGCGAG 
GAGCAGAAGA TAAGCGACAT GGTGTATTCC CTATTCCGCA TGCTGCTCCA CCATGCCATT 
AAGCATGAAT ACGGTGGCTG GAGAGTTTGG GTAGATACCC TGGCAATCGT CCACTCCAAG 
GTGTCTTACG AGGAGTTTAA GCTCCAGTTT GCCCAGATGT ACGAGCACTA CGAGCGCCAG 
CGAACGGACA ATATCACAGA TCCTGCCCTG CGTCAGGCTA GACCCATCAG CACGATCAGT 
GGTTGGGAGC GAGAGGAGCT GCATCAGCAG CAGAACGGTG GATCTGCAGC AGCAGTGGCT 
ACCAATCAAA CGGCAGCTGT TAAAGGTTCT GTTTCCATTG CCTCACTGGA AGATGTGCCA 
CCGGTGGTTG AGGAAGAGGT GGAGGAACTG GAGCTGGAGG AAGTAGAGAT TCAAGAGGGT 
CCTATAACCG AAGAAACCGA ACAAAAATCG GTGATAGCCA ACATTTCCGA CGTCTACAAT 
GAGCAGCTCA AAACCGATGC CACGTGCAAT GGCAATTTGG AGGATGTCAA AGAGGAGGAG 
CCTGTACAAC AGCAAATCGG AGACCTGGAG AAGCAACCAG AGCCATCTAC TCCTTTGGGA 
GCACTCAGGG AAACCCTACA ACTTGGTGAT GATATGGATG TGGAAGAACT GGAGCTGGCT 
ACCGCCAAGG ATGCCCTCAA TGCGGAACAG CATGTGTCGC GAGTTCTTCA GGCTTCCGAA 
GCGGCACTTA ACGATTGCAA AATGGCAGTC GATGATGTCC TGCAGGAGTC ATCCTCCGTC 
CTCAAGGACG AGGAGATCGA ACTGGCCGTC AACGAGGTTG TTCAGGGTGT GCTCAATAAC 
GAGAAGAAAA CTCAATCCCA GGACAACAAG GATAACAAGG AACAGCCGGG CGAACAGGAT 
GTAAACGTCA GTCTCCTAAA CAGCAAGAAT CTGCTCAACA ATAATAATAA TAACAATAAC 
AACAGTCCGA GTCCAACGCC CACAACGGCG ACTGCGACGG CGGAGACGGA AGCGGAAACA 
GAAGTCAATG CCAATGAGAT CGTGAGCAGC ACAGAGGCGC CCAAGGCGGA AACGGAAACG 
AGCGTAGCAC CGGAAGTAGA AACACCGGAA ACGGCCAAGC CAAGCCCCAT TGTCCCCAGC 
CCCGTATTAG CAACCAATCA AAAGACTGAA GATGCAGCCA ACAAGCTGAA CAACAACGAG 
AAGCTGGCCG AGATCAGCGC CAGTCCCGAG CCACCCATTG TCGTGGAAAC GCCAGAAGCT 
GATCTTCTTC AGCTTTCCGA TTCCGAAACA AAACCGAATA AGGAAACCGA AGCTGAAGAT 
TCGGTGGCGC TGGCCGTTAG AGATATTGTC GAGCAACTCA TCGACAAGGT CATCGATGCC 
ACGGAAGCGG AATCGGCCAG CGAAACCAAA ACGGAAACGA ATAATAATGA AATACCCAAG 
AAGGAGAAGC AGACGTCAGA AGAGCCTGAG GATGTGGAAA CTGCAGAGAC TTTGGCCGCT 
GCCGCCAAGG AAATTGTCCA AGAGGTTGTG GAAGCAGCTC TCGTAATGGT CCAAGAGGAG 
AGCACTCAAG AGAAGCCAGA AAAGGGGGCG AACTCCGAAG AGGAGAAAAA CGAAATTGGC 
AAGGAAGAGA TTCTTCTGCA GCTGGAGGAA AAACCAGCGT CTACTGAAGT GCAGGAAACC 
AAGATCGAAG GAGATCTAAA GAAACCAGAA GATCCAAAAG GTCACAGCTC TGTAGAACCA 
AAGACCCCGA ACCTTGAGGA ACCGAAACCC CAGGAAACTG AACAACAAAA GTCGCAGGAG 
GTCGCCGAAG AACTACCTCA AAAACCAGAG GAGCAGGTGG TGGCCATTGT CAACCAAGTC 
CTGGACACTC TGGTAGACGA TACGGTCAAG GCAGTGGCAG CCGAGCAAAC CACTCAAACA 
TCGCCAGCCC CCGAAGAGCA ATCGCCACAA ATACTGGCAA TGGAATCACC AGCAACGTCG 
GTGCGGGTCA AACCCACTGA GGTGGATTCC ACCACCCAGA CTACGCCGAA GAACGAGGCT 
GGATCCAGTC TATTGGTTGA GCAGGTGCAG CAAGTCCTCC AGGAGGATGA CGCCCAGCAA 
TCAGCCGGCA TGACCATCGA AGATGAGGAT TACTCTAATC AGCAGGCAGC TGCGGCAGTT 
GAAAATGCCA ACAGCAGCCA ATTGGATGCC AATCATTATG GTCCCGGTAA TCCAGAATCC 
AAACAACAGC AACAGCGCTC CAAATCGGGA TCGACCCGAC CTATGTTCAG TCCGGGACCT 
ACACGTCCGC CCTTCCGAAT ACCGGAATTC AAGTGGTCCT ACATCCATCA GCGATTGCTC 
AGCGATGTCC TCTTTTCGCT GGAAACGGAC ATTCAGGTGT GGCGCAGTCA TTCGACCAAA 
AGCGTCCTGG ACTTTGTCAA CTCCAGCGAG AATGCAATTT TTGTGGTGAA CACGGTGCAT 
TTAATATCGC AGCTAGCGGA TAATCTGATT ATTGCCTGCG GAGGATTGCT GCCTCTTTTG 
GCCAGCGCCA CGTCACCCAA TTCCGAATTG GATGTGCTGG AGCCAACGCA AGGCATGCCT 
TTGGAGGTGG CCGTATCTTT CCTGCAGCGT CTGGTCAACA TGGCGGATGT CCTGATCTTT 
GCCACATCCC TAAACTTCGG CGAGTTGGAG GCGGAGAAGA ACATGTCCAG TGGCGGCATA 
CTGCGTCAGT GCCTGCGATT GGTTTGCACC TGTGCGGTAA GGAATTGCTT GGAGTGCAAG 
GAGCGAACGC GTTACAATGT GGGCGCCTTG GCGAGAGATG TCCCGGGTGC AGCCCATTTG 
CAGGCCCTCA TCCGCGGAGC TCAAGCATCG CCCAAGAACA TTGTCGAGTC AATAACCGGC 
CAATTATCGC CTGTCAAGGA TCCCGAGAAG CTGCTCCAGG ACATGGACGT CAATCGCCTG 
CGCGCAGTCA TCTATCGCGA TGTGGAGGAG ACTAAGCAGG CGCAGTTCCT CTCGCTGGCG 
ATAGTGTACT TCATATCGGT GCTGATGGTG TCCAAGTATC GTGATATCCT GGAGCCGCCG 
GCAGAGCCGC AAATCCAGCG TCAATCGCCA GTGCTGCAAC GCACGGCAGG CGGCGGTGGT 
CGTCAAATCC AGGATAGTGA CTACGAAATA ATTGTCGTCG ATGAGAACAA TCCATCGGTT 
TTGGCGGATA ATGATTCACA TTCCAGTGGA CCACCATCCA TAAAGAGCGT TGACTCGGAT 
GTGGGCTCCC TGAACATGAA CTCCACGGAA AACGAAGTGC CCGAGGTGGA GTCATCCAGC 
GAGATTCTAA TCGATGACCA CAAACCGAGT CACTCGAACG ACGAAAGCTG GACGGATGTG 
AATCTCAATG AGGATGCTGC CGTTCAGGCG GCAAGTGCCG GAATAGTTGT AGGTTTGGTG 
GATAACAGAG GTAATGTTAT ATCCGACAAA CACGATCCGT CCCATCACAA CCAACAACAG 
CAGCAGCAGG CGGGAATTAT TGGTCAGCAG CAGCAACATG GATCACTTGG TCATTCGGAG 
CGGGGTGACA AACCCGATTC GGAGATTTCG GTGGTGCGAG TGCCTGATGG TTATGGTGGT 
GCTGGTTCCG GTGGTGGCGT GAATTCCGGA CAAGGTCAGG GCGTTCCATC CAATCAGCGT 
CCTCGTCCCG AGGAGTTGCC CATGAAGGCG CCCGCCTTGG TGGCCCAACT GCCGCTGACC 
ACACCTTCGC GAGAGGCCAG TCTCACCCAG AAGCTGGAAA TCGCATTGGG GCCAGTGTGT 
CCGCTGCTCC GTGAAATCAT GGTGGACTTT GCGCCGTTCC TTTCGAAAAC CCTAGTCGGC 
TCCCATGGCC AGGAATTGCT GATGGAGGGC AAGGGTCTGA CGACCTTTAA GAACTCGCAT 
TCCGTCGTAG AGCTGGTCAT GTTGCTCTGC TCCCAGGAGT GGCAAAATAG CCTACAAAAG 
CACGCAGGAT TAGCCTTTAT TGAGCTAATC AATGAGGGTC GACTGCTTTC GCACGCCATG 
AAGGATCACA TTGTTCGGGT GGCCAATGAG GCGGAGTTCA TCCTCAATAG AATGCGAGCG 
GATGATGTCC TCAAGCATGC CGACTTTGAA TCGCAATGTG CACAGACACT TTTGGAGCGC 
AGGGAGGAGG AGCGAATGTG CGATCACTTG ATAACCGCCG CACGTCGCAG AGACAATGTG 
ATTGCCAGCC GACTGCTGGA GAAGGTACGA AACATCATGT GTAATCGCCA CGGAGCCTGG 
GGTGATTCCA GCTCCACATC CAGTGGTGGC GCCATCGTTG GAGCGGTGCA AAAGAGTCCC 
TACTGGAAGC TGGATGCCTG GGAGGATGAT GCCAGACGTC GGAAACGGAT GGTGCAAAAT 
CCGCGCGGAT CCTCGCATCC ACAGGCCACA CTGAAGGCGG CTTTGGAAAA TGGCGGACCT 
GAAGATGCCA TCCTGCAGAC TCGCGACGAG TTCCACACCC AAATAGCCGT CTCGCGGACT 
CATCCATCCG GTCAGCACAA TGGTGAGCTA CTGGACGACG CCGAGCTCTT GATTGAGGAT 
CGGGAGTTGG ATCTGGATCT CACGGGTCCC GTCAACATTA GCACCAAGGC GAGATTGATA 
GCCCCCGGTC TGGTGGCACC CGGCACCGTC TCAATAACCA GCACTGAAAT GTTCTTTGAG 
GTGGATGAAG AGCATCCCGA GTTCCAGAAG ATCGATGGCG AAGTTCTTAA GTACTGTGAC 
CATTTGCACG GCAAATGGTA CTTCTCCGAA GTGAGGGCCA TCTTCTCGAG GCGTTATCTC 
CTGCAAAATG TGGCCCTCGA GATCTTTTTG GCCAGCCGTA CGTCCATATT GTTTGCCTTT 
CCCGATCAGC ATACGGTCAA GAAGGTAATC AAGGCCCTGC CCCGTGTGGG AGTGGGCATC 
AAGTATGGAA TACCGCAGAC GAGGAGGGCC TCAATGATGT CGCCCCGTCA ACTGATGCGC 
AATTCGAACA TGACCCAAAA GTGGCAGCGT CGCGAGATTA GCAACTTTGA GTATCTTATG 
TTCCTGAACA CGATCGCCGG CAGGACGTAC AACGATCTAA ATCAGTATCC CATCTTTCCG 
TGGGTGCTGA CCAACTATGA GTCCAAGGAT TTGGATCTCA GCCTGCCGTC AAACTACAGG 
GATCTGTCGA AACCGATTGG CGCCCTGAAT CCATCGCGCA GAGCCTATTT CGAGGAGCGT 
TACGAGAGCT GGGACAGTGA CACCATACCG CCCTTCCACT ATGGTACGCA TTATTCCACG 
GCAGCATTTA CGCTGAACTG GTTGGTGCGC GTGGAACCGT TTACCACCAT GTTCCTGGCC 
CTGCAGGGCG GCAAATTTGA TTATCCGGAC AGGCTGTTCA GCTCGGTGTC GCTGTCCTGG 
AAGAATTGCC AACGTGATAC ATCGGATGTG AAGGAACTGA TACCAGAATG GTATTTCCTG 
CCCGAAATGT TTTACAATTC ATCAGGCTAT CGACTGGGTC ATCGCGAGGA TGGTGCCCTA 
GTGGATGACA TTGAACTACC ACCGTGGGCC AAGAGCCCCG AAGAATTCGT CCGCATCAAT 
CGCATGGCAC TGGAGTCGGA GTTCGTGTCC TGCCAGCTGC ACCAGTGGAT CGATTTGATC 
TTTGGCTACA AGCAACGCGG TCCCGAGGCC ATTAGGGCAA CCAACGTGTT CTACTATTTG 
ACATATGAGG GCAGCGTTGA CTTGGATGGC GTTTTGGATC CGGTGATGCG CGAAGCCGTC 
GAGAATCAAA TCCGCAACTT TGGCCAAACA CCTAGTCAAC TGCTAATGGA ACCGCATCCG 
CCGCGCAGCT CTGCGATGCA TCTATCGCCT ATGATGTTCA GTGCGATGCC GGAGGATCTC 
TGCCAGATGC TCAAGTTCTA TCAGAACTCA CCAGTCATTC ACATCTCGGC CAACACCTAT 
CCGCAACTGT CGTTGCCATC TGTGGTCACG GTAACGGCGG GTCATCAGTT TGCGGTGAAT 
CGATGGAACT GCAACTACAC CGCCTCCGTC CAGAGTCCCA GCTACGCCGA ATCGCCCCAA 
TCGCCGGGTT CCAATCAACC ACTGACCATC GATCCGGTTC TGGCTGTTCA TGGCACCAAC 
AACAATAGCA ATGCGGCCAG CCGACGTCAT TTGGGCGATA ACTTTAGCCA GATGCTAAAG 
ATACGCTCCA ACTGCTTTGT TACCACAGTG GACAGTCGGT TCCTAATCGC ATGCGGATTC 
TGGGACAACA GTTTCCGCGT CTTTGCCACC GAAACGGCGA AAATCGTGCA GATTGTATTC 
GGTCACTTTG GTGTGGTAAC GTGCATGGCA CGTTCCGAGT GCAACATCAC ATCAGACTGC 
TACATCGCCT CCGGATCGGC GGACTGCACG GTGCTTCTGT GGCACTGGAA TGCCCGAACC 
CAGAGCATTG TGGGCGAGGG CGATGTGCCC ACACCACGGG CCACCTTGAC GGGTCACGAA 
CAGGCCGTGA CATCGGTGGT GATTAGTGCC GAGTTGGGTC TGGTTGTGTC TGGATCATCA 
AATGGACCCG TACTTATCCA TACGACTTTT GGCGACCTGC TGCGCTCACT GGATCCACCA 
GCGGAGTTCC ATTCCCCGGA ACTGATAACC ATGTCCCGCG AGGGTTTCAT TGTCATCAAC 
TACGATAAGG GCAATGTGGC CGCCTACACC ATCAATGGCA AGAAACTGCG CCACGAGACG 
CACAACGACA ATCTACAGTG CATGCTACTG TCGCGCGATG GTGAATACCT GATGACCGCC 
GGTGATCGCG GCATCGTGGA GGTGTGGCGC ACCTTCAACC TAGCACCACT TTATGCCTTC 
CCCGCATGCA ACGCGGGCAT CCGATCTTTG GCTCTCACCC ACGATCAGAA ATACCTTCTG 
GCCGGACTTT CGACGGGCTC GATCATAGTA TTCCACATCG ACTTCAATCG CTGGCACCAC 
GAGTACCAGC AGCGCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rg ( NM_001297950.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284879.1 Drosophila melanogaster rugose (rg), transcript variant Q, mRNA.

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CloneID	OFa22215
Clone ID Related Accession (Same CDS sequence)	NM_001297950.1
Accession Version	NM_001297950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform Q
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGTTG 
TTCTGTTTCT CGTTTCTCAC GTGGCAGCTG CTGGATCACG TCCTCTTCAA TCCAGCCCTG 
TGGATATACA CACCCGCCAA TGTCCAGGCG CGACTATACT CCTACTTGGC CACCGAGTTC 
CTCTCGGACA CGCAGATCTA CAGCAATGTG AGGAGGGTCA GCACGGTACT GCAGACAGTG 
CACACCCTAA AGTACTACTA CTGGGTGGTC AATCCACGGG CTAAGAGCGG CATCATTCCC 
AAGGGACTGG ATGGACCTCG TCCGGCTCAA AAGGACATCC TGGCCATCCG GGCATACATC 
CTGCTGTTCC TGAAGCAGCT CATAATGATC GGCAATGGCG TGAAGGAGGA CGAGCTGCAG 
AGCATACTCA ACTATCTGAC CACCATGCAC GAGGACGAGA ACCTGCACGA CGTGCTGCAG 
ATGCTCATCT CGCTGATGTC GGAGCATCCC AGCTCCATGG TACCTGCCTT TGATGTGAAG 
CACGGCGTGC GCAGCATCTT CAAGTTGCTG GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG 
GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC CGCAGCACCC ACAAACGCAA GTACGATGTG 
ATGTCACCAC ACAATCTGTA CACCCTTTTA GCGGAGCGAC TGCTTCTCTA TGAGGAATCA 
CTGTCCCTGC CCACATACAA TGTTCTCTAT GAGATCATGA CGGAGCACAT CTCGCAGCAG 
ATTCTATACA CACGCCATCC GGAACCAGAG AGTCACTACC GTCTGGAGAA TCCAATGATC 
CTCAAGGTGG TGGCCACCCT GATCCGGCAA TCAAAGCAGA CGGAATCGCT GATCGACGTT 
AAAAAGCTAT TCCTGCAGGA TATGACCCTG TTGTGCAATA GCAATCGGGA GAACCGGCGC 
ACAGTGCTCC AGATGTCCGT GTGGCAGGAG TGGCTCATTG CGATGGCGTA CATTCATCCA 
AAGAGCAGCG AGGAGCAGAA GATAAGCGAC ATGGTGTATT CCCTATTCCG CATGCTGCTC 
CACCATGCCA TTAAGCATGA ATACGGTGGC TGGAGAGTTT GGGTAGATAC CCTGGCAATC 
GTCCACTCCA AGGTGTCTTA CGAGGAGTTT AAGCTCCAGT TTGCCCAGAT GTACGAGCAC 
TACGAGCGCC AGCGAACGGA CAATATCACA GATCCTGCCC TGCGTCAGGC TAGACCCATC 
AGCACGATCA GTGGTTGGGA GCGAGAGGAG CTGCATCAGC AGCAGAACGG TGGATCTGCA 
GCAGCAGTGG CTACCAATCA AACGGCAGCT GTTAAAGGTT CTGTTTCCAT TGCCTCACTG 
GAAGATGTGC CACCGGTGGT TGAGGAAGAG GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG 
ATTCAAGAGG GTCCTATAAC CGAAGAAACC GAACAAAAAT CGGTGATAGC CAACATTTCC 
GACGTCTACA ATGAGCAGCT CAAAACCGAT GCCACGTGCA ATGGCAATTT GGAGGATGTC 
AAAGAGGAGG AGCCTGTACA ACAGCAAATC GGAGACCTGG AGAAGCAACC AGAGCCATCT 
ACTCCTTTGG GAGCACTCAG GGAAACCCTA CAACTTGGTG ATGATATGGA TGTGGAAGAA 
CTGGAGCTGG CTACCGCCAA GGATGCCCTC AATGCGGAAC AGCATGTGTC GCGAGTTCTT 
CAGGCTTCCG AAGCGGCACT TAACGATTGC AAAATGGCAG TCGATGATGT CCTGCAGGAG 
TCATCCTCCG TCCTCAAGGA CGAGGAGATC GAACTGGCCG TCAACGAGGT TGTTCAGGGT 
GTGCTCAATA ACGAGAAGAA AACTCAATCC CAGGACAACA AGGATAACAA GGAACAGCCG 
GGCGAACAGG ATGTAAACGT CAGTCTCCTA AACAGCAAGA ATCTGCTCAA CAATAATAAT 
AATAACAATA ACAACAGTCC GAGTCCAACG CCCACAACGG CGACTGCGAC GGCGGAGACG 
GAAGCGGAAA CAGAAGTCAA TGCCAATGAG ATCGTGAGCA GCACAGAGGC GCCCAAGGCG 
GAAACGGAAA CGAGCGTAGC ACCGGAAGTA GAAACACCGG AAACGGCCAA GCCAAGCCCC 
ATTGTCCCCA GCCCCGTATT AGCAACCAAT CAAAAGACTG AAGATGCAGC CAACAAGCTG 
AACAACAACG AGAAGCTGGC CGAGATCAGC GCCAGTCCCG AGCCACCCAT TGTCGTGGAA 
ACGCCAGAAG CTGATCTTCT TCAGCTTTCC GATTCCGAAA CAAAACCGAA TAAGGAAACC 
GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT AGAGATATTG TCGAGCAACT CATCGACAAG 
GTCATCGATG CCACGGAAGC GGAATCGGCC AGCGAAACCA AAACGGAAAC GAATAATAAT 
GAAATACCCA AGAAGGAGAA GCAGACGTCA GAAGAGCCTG AGGATGTGGA AACTGCAGAG 
ACTTTGGCCG CTGCCGCCAA GGAAATTGTC CAAGAGGTTG TGGAAGCAGC TCTCGTAATG 
GTCCAAGAGG AGAGCACTCA AGAGAAGCCA GAAAAGGGGG CGAACTCCGA AGAGGAGAAA 
AACGAAATTG GCAAGGAAGA GATTCTTCTG CAGCTGGAGG AAAAACCAGC GTCTACTGAA 
GTGCAGGAAA CCAAGATCGA AGGAGATCTA AAGAAACCAG AAGATCCAAA AGGTCACAGC 
TCTGTAGAAC CAAAGACCCC GAACCTTGAG GAACCGAAAC CCCAGGAAAC TGAACAACAA 
AAGTCGCAGG AGGTCGCCGA AGAACTACCT CAAAAACCAG AGGAGCAGGT GGTGGCCATT 
GTCAACCAAG TCCTGGACAC TCTGGTAGAC GATACGGTCA AGGCAGTGGC AGCCGAGCAA 
ACCACTCAAA CATCGCCAGC CCCCGAAGAG CAATCGCCAC AAATACTGGC AATGGAATCA 
CCAGCAACGT CGGTGCGGGT CAAACCCACT GAGGTGGATT CCACCACCCA GACTACGCCG 
AAGAACGAGG CTGGATCCAG TCTATTGGTT GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT 
GACGCCCAGC AATCAGCCGG CATGACCATC GAAGATGAGG ATTACTCTAA TCAGCAGGCA 
GCTGCGGCAG TTGAAAATGC CAACAGCAGC CAATTGGATG CCAATCATTA TGGTCCCGGT 
AATCCAGAAT CCAAACAACA GCAACAGCGC TCCAAATCGG GATCGACCCG ACCTATGTTC 
AGTCCGGGAC CTACACGTCC GCCCTTCCGA ATACCGGAAT TCAAGTGGTC CTACATCCAT 
CAGCGATTGC TCAGCGATGT CCTCTTTTCG CTGGAAACGG ACATTCAGGT GTGGCGCAGT 
CATTCGACCA AAAGCGTCCT GGACTTTGTC AACTCCAGCG AGAATGCAAT TTTTGTGGTG 
AACACGGTGC ATTTAATATC GCAGCTAGCG GATAATCTGA TTATTGCCTG CGGAGGATTG 
CTGCCTCTTT TGGCCAGCGC CACGTCACCC AATTCCGAAT TGGATGTGCT GGAGCCAACG 
CAAGGCATGC CTTTGGAGGT GGCCGTATCT TTCCTGCAGC GTCTGGTCAA CATGGCGGAT 
GTCCTGATCT TTGCCACATC CCTAAACTTC GGCGAGTTGG AGGCGGAGAA GAACATGTCC 
AGTGGCGGCA TACTGCGTCA GTGCCTGCGA TTGGTTTGCA CCTGTGCGGT AAGGAATTGC 
TTGGAGTGCA AGGAGCGAAC GCGTTACAAT GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT 
GCAGCCCATT TGCAGGCCCT CATCCGCGGA GCTCAAGCAT CGCCCAAGAA CATTGTCGAG 
TCAATAACCG GCCAATTATC GCCTGTCAAG GATCCCGAGA AGCTGCTCCA GGACATGGAC 
GTCAATCGCC TGCGCGCAGT CATCTATCGC GATGTGGAGG AGACTAAGCA GGCGCAGTTC 
CTCTCGCTGG CGATAGTGTA CTTCATATCG GTGCTGATGG TGTCCAAGTA TCGTGATATC 
CTGGAGCCGC CGGCAGAGCC GCAAATCCAG CGTCAATCGC CAGTGCTGCA ACGCACGGCA 
GGCGGCGAAG CCGCCAGTGC GCGTCCTTTG TTCCCCCAAT GGTCGCATCA TGTTTACCCG 
CAATTCCTGC CCGAATCGCA CCAGAATCAC AACAGCAACA TGCAACACCA GCAGCAGCAG 
CAGCAGCAAC AGCAACATCA GCAGCAGCAA CACTACTACC AGCAACAGCA GCAACAGCAG 
GTTGTCCACA ATCATAGCCA TCATCACATG ACTGCCGCTT ACTACCAACA GCAGCAACAC 
CAGCAAGTTG CAGCAGGACA GCAGCAACAT TCGCCCACTC CTCTGGCCAC ACATTCGACC 
AGCTCCTCGG CCAGTTCGAC GGCCACATCT CAGCCAGCGT CGAGCTCCTC GCTCTCCAGC 
CTGGCATCCC AAAGTCAGCA GCAATCGCAT CGCCAGTTGC ACAAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAACA ACAGCCACAT TACCATCCAC ATCAGCCGCA CTATGGACTG 
ATCAATGGGC ACCAGCAACA TCCGCAGCTA AATGGTAAAC ATTATGCGGA AAATGGATCG 
ACAGCGGGAT ACCATCCGCA TTCGCATCCA CATCCACACG GTGGTTATAT GCGAAATGGT 
GATACGTCGA CATTGCAACA GAATGGTATG CCGGACTATC AGGCAGTGGG TCTGATGAAT 
GGTCATGGCT CTGGTGGAAC GGGTAATAAC AATAACTCCA GCCTGATGAA CAACATGCGA 
AATCTGCGAA ATGGCGGAGG AGCTAACTCA TCATCTTCGC CACCGGGAAG CCATCAAGTT 
ATCCAAGGTG TTGCAACAGC GGGAGCTGTT AATGCAAATG CTGCTGTTGG TGTTGGCATG 
GGCGGAGCCG TTGGTGTGGG TATGGGGGGT GTGGCAGGTG GTTTGGATGG AGGCGTGGTC 
TACAAGACGA GCGCCATAAA TAATAATTAC CGCTACAATG GACGCAACGC ATCCGCTGGA 
ACAGGTGGTC GTCAAATCCA GGATAGTGAC TACGAAATAA TTGTCGTCGA TGAGAACAAT 
CCATCGGTTT TGGCGGATAA TGATTCACAT TCCAGTGGAC CACCATCCAT AAAGGCCAAC 
CAGACAACAA CAATAGCAAC AACAACTACT ACAACAGCAA CAACCCATAT TAACAACAAC 
AACACTAAGA CTACAACATC AAACGCTCCA ACAGCAACAA TTAAAATTCA GCAACAACCA 
TTGTCACCAC CAAAGCCGTT GGTGCCACAA AAATTGCCAA AGAGCGTTGA CTCGGATGTG 
GGCTCCCTGA ACATGAACTC CACGGAAAAC GAAGTGCCCG AGGTGGAGTC ATCCAGCGAG 
ATTCTAATCG ATGACCACAA ACCGAGTCAC TCGAACGACG AAAGCTGGAC GGATGTGAAT 
CTCAATGAGG ATGCTGCCGT TCAGGCGGCA AGTGCCGGAA TAGTTGTAGG TTTGGTGGAT 
AACAGAGGTA ATGTTATATC CGACAAACAC GATCCGTCCC ATCACAACCA ACAACAGCAG 
CAGCAGGCGG GAATTATTGG TCAGCAGCAG CAACATGGAT CACTTGGTCA TTCGGAGCGG 
GGTGACAAAC CCGATTCGGA GATTTCGGTG GTGCGAGTGC CTGATGGTTA TGGTGGTGCT 
GGTTCCGGTG GTGGCGTGAA TTCCGGACAA GGTCAGGGCG TTCCATCCAA TCAGCGTCCT 
CGTCCCGAGG AGTTGCCCAT GAAGGCGCCC GCCTTGGTGG CCCAACTGCC GCTGACCACA 
CCTTCGCGAG AGGCCAGTCT CACCCAGAAG CTGGAAATCG CATTGGGGCC AGTGTGTCCG 
CTGCTCCGTG AAATCATGGT GGACTTTGCG CCGTTCCTTT CGAAAACCCT AGTCGGCTCC 
CATGGCCAGG AATTGCTGAT GGAGGGCAAG GGTCTGACGA CCTTTAAGAA CTCGCATTCC 
GTCGTAGAGC TGGTCATGTT GCTCTGCTCC CAGGAGTGGC AAAATAGCCT ACAAAAGCAC 
GCAGGATTAG CCTTTATTGA GCTAATCAAT GAGGGTCGAC TGCTTTCGCA CGCCATGAAG 
GATCACATTG TTCGGGTGGC CAATGAGGCG GAGTTCATCC TCAATAGAAT GCGAGCGGAT 
GATGTCCTCA AGCATGCCGA CTTTGAATCG CAATGTGCAC AGACACTTTT GGAGCGCAGG 
GAGGAGGAGC GAATGTGCGA TCACTTGATA ACCGCCGCAC GTCGCAGAGA CAATGTGATT 
GCCAGCCGAC TGCTGGAGAA GGTACGAAAC ATCATGTGTA ATCGCCACGG AGCCTGGGGT 
GATTCCAGCT CCACATCCAG TGGTGGCGCC ATCGTTGGAG CGGTGCAAAA GAGTCCCTAC 
TGGAAGCTGG ATGCCTGGGA GGATGATGCC AGACGTCGGA AACGGATGGT GCAAAATCCG 
CGCGGATCCT CGCATCCACA GGCCACACTG AAGGCGGCTT TGGAAAATGG CGGACCTGAA 
GATGCCATCC TGCAGACTCG CGACGAGTTC CACACCCAAA TAGCCGTCTC GCGGACTCAT 
CCATCCGGTC AGCACAATGG TGAGCTACTG GACGACGCCG AGCTCTTGAT TGAGGATCGG 
GAGTTGGATC TGGATCTCAC GGGTCCCGTC AACATTAGCA CCAAGGCGAG ATTGATAGCC 
CCCGGTCTGG TGGCACCCGG CACCGTCTCA ATAACCAGCA CTGAAATGTT CTTTGAGGTG 
GATGAAGAGC ATCCCGAGTT CCAGAAGATC GATGGCGAAG TTCTTAAGTA CTGTGACCAT 
TTGCACGGCA AATGGTACTT CTCCGAAGTG AGGGCCATCT TCTCGAGGCG TTATCTCCTG 
CAAAATGTGG CCCTCGAGAT CTTTTTGGCC AGCCGTACGT CCATATTGTT TGCCTTTCCC 
GATCAGCATA CGGTCAAGAA GGTAATCAAG GCCCTGCCCC GTGTGGGAGT GGGCATCAAG 
TATGGAATAC CGCAGACGAG GAGGGCCTCA ATGATGTCGC CCCGTCAACT GATGCGCAAT 
TCGAACATGA CCCAAAAGTG GCAGCGTCGC GAGATTAGCA ACTTTGAGTA TCTTATGTTC 
CTGAACACGA TCGCCGGCAG GACGTACAAC GATCTAAATC AGTATCCCAT CTTTCCGTGG 
GTGCTGACCA ACTATGAGTC CAAGGATTTG GATCTCAGCC TGCCGTCAAA CTACAGGGAT 
CTGTCGAAAC CGATTGGCGC CCTGAATCCA TCGCGCAGAG CCTATTTCGA GGAGCGTTAC 
GAGAGCTGGG ACAGTGACAC CATACCGCCC TTCCACTATG GTACGCATTA TTCCACGGCA 
GCATTTACGC TGAACTGGTT GGTGCGCGTG GAACCGTTTA CCACCATGTT CCTGGCCCTG 
CAGGGCGGCA AATTTGATTA TCCGGACAGG CTGTTCAGCT CGGTGTCGCT GTCCTGGAAG 
AATTGCCAAC GTGATACATC GGATGTGAAG GAACTGATAC CAGAATGGTA TTTCCTGCCC 
GAAATGTTTT ACAATTCATC AGGCTATCGA CTGGGTCATC GCGAGGATGG TGCCCTAGTG 
GATGACATTG AACTACCACC GTGGGCCAAG AGCCCCGAAG AATTCGTCCG CATCAATCGC 
ATGGCACTGG AGTCGGAGTT CGTGTCCTGC CAGCTGCACC AGTGGATCGA TTTGATCTTT 
GGCTACAAGC AACGCGGTCC CGAGGCCATT AGGGCAACCA ACGTGTTCTA CTATTTGACA 
TATGAGGGCA GCGTTGACTT GGATGGCGTT TTGGATCCGG TGATGCGCGA AGCCGTCGAG 
AATCAAATCC GCAACTTTGG CCAAACACCT AGTCAACTGC TAATGGAACC GCATCCGCCG 
CGCAGCTCTG CGATGCATCT ATCGCCTATG ATGTTCAGTG CGATGCCGGA GGATCTCTGC 
CAGATGCTCA AGTTCTATCA GAACTCACCA GTCATTCACA TCTCGGCCAA CACCTATCCG 
CAACTGTCGT TGCCATCTGT GGTCACGGTA ACGGCGGGTC ATCAGTTTGC GGTGAATCGA 
TGGAACTGCA ACTACACCGC CTCCGTCCAG AGTCCCAGCT ACGCCGAATC GCCCCAATCG 
CCGGGTTCCA ATCAACCACT GACCATCGAT CCGGTTCTGG CTGTTCATGG CACCAACAAC 
AATAGCAATG CGGCCAGCCG ACGTCATTTG GGCGATAACT TTAGCCAGAT GCTAAAGATA 
CGCTCCAACT GCTTTGTTAC CACAGTGGAC AGTCGGTTCC TAATCGCATG CGGATTCTGG 
GACAACAGTT TCCGCGTCTT TGCCACCGAA ACGGCGAAAA TCGTGCAGAT TGTATTCGGT 
CACTTTGGTG TGGTAACGTG CATGGCACGT TCCGAGTGCA ACATCACATC AGACTGCTAC 
ATCGCCTCCG GATCGGCGGA CTGCACGGTG CTTCTGTGGC ACTGGAATGC CCGAACCCAG 
AGCATTGTGG GCGAGGGCGA TGTGCCCACA CCACGGGCCA CCTTGACGGG TCACGAACAG 
GCCGTGACAT CGGTGGTGAT TAGTGCCGAG TTGGGTCTGG TTGTGTCTGG ATCATCAAAT 
GGACCCGTAC TTATCCATAC GACTTTTGGC GACCTGCTGC GCTCACTGGA TCCACCAGCG 
GAGTTCCATT CCCCGGAACT GATAACCATG TCCCGCGAGG GTTTCATTGT CATCAACTAC 
GATAAGGGCA ATGTGGCCGC CTACACCATC AATGGCAAGA AACTGCGCCA CGAGACGCAC 
AACGACAATC TACAGTGCAT GCTACTGTCG CGCGATGGTG AATACCTGAT GACCGCCGGT 
GATCGCGGCA TCGTGGAGGT GTGGCGCACC TTCAACCTAG CACCACTTTA TGCCTTCCCC 
GCATGCAACG CGGGCATCCG ATCTTTGGCT CTCACCCACG ATCAGAAATA CCTTCTGGCC 
GGACTTTCGA CGGGCTCGAT CATAGTATTC CACATCGACT TCAATCGCTG GCACCACGAG 
TACCAGCAGC GCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284879.1
CDS	1089..12446
Translation	MADIMRPPYSEIKRPDEIVRMTTADNLKFAVLIGLIEVGQVTNREVVNTVLHLLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSHFQLLPATLGASALSGGSGSGTGSGTGNDASAAAAAAVAAGQQQQLQLQFQILAAEQEARAIDWSDEKLDLNAAFVKIRAVLTARNAVTLAGSSSTGTTAVATAAAAAAAAGAGAGTTAAATSAAAAAAATQNENDAAVGQQQHATHHHATAATGSADDPLGHLPTGNASSSSSSFEQLRRMSSVSSLNSMVGSADTEEVNQLKAVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLFCFSFLTWQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGEAASARPLFPQWSHHVYPQFLPESHQNHNSNMQHQQQQQQQQQHQQQQHYYQQQQQQQVVHNHSHHHMTAAYYQQQQHQQVAAGQQQHSPTPLATHSTSSSASSTATSQPASSSSLSSLASQSQQQSHRQLHKQQQQQQQQQQQQQPHYHPHQPHYGLINGHQQHPQLNGKHYAENGSTAGYHPHSHPHPHGGYMRNGDTSTLQQNGMPDYQAVGLMNGHGSGGTGNNNNSSLMNNMRNLRNGGGANSSSSPPGSHQVIQGVATAGAVNANAAVGVGMGGAVGVGMGGVAGGLDGGVVYKTSAINNNYRYNGRNASAGTGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKANQTTTIATTTTTTATTHINNNNTKTTTSNAPTATIKIQQQPLSPPKPLVPQKLPKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_001297950.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant Q, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297950.1

ORF Insert Sequence:

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGTTG 
TTCTGTTTCT CGTTTCTCAC GTGGCAGCTG CTGGATCACG TCCTCTTCAA TCCAGCCCTG 
TGGATATACA CACCCGCCAA TGTCCAGGCG CGACTATACT CCTACTTGGC CACCGAGTTC 
CTCTCGGACA CGCAGATCTA CAGCAATGTG AGGAGGGTCA GCACGGTACT GCAGACAGTG 
CACACCCTAA AGTACTACTA CTGGGTGGTC AATCCACGGG CTAAGAGCGG CATCATTCCC 
AAGGGACTGG ATGGACCTCG TCCGGCTCAA AAGGACATCC TGGCCATCCG GGCATACATC 
CTGCTGTTCC TGAAGCAGCT CATAATGATC GGCAATGGCG TGAAGGAGGA CGAGCTGCAG 
AGCATACTCA ACTATCTGAC CACCATGCAC GAGGACGAGA ACCTGCACGA CGTGCTGCAG 
ATGCTCATCT CGCTGATGTC GGAGCATCCC AGCTCCATGG TACCTGCCTT TGATGTGAAG 
CACGGCGTGC GCAGCATCTT CAAGTTGCTG GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG 
GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC CGCAGCACCC ACAAACGCAA GTACGATGTG 
ATGTCACCAC ACAATCTGTA CACCCTTTTA GCGGAGCGAC TGCTTCTCTA TGAGGAATCA 
CTGTCCCTGC CCACATACAA TGTTCTCTAT GAGATCATGA CGGAGCACAT CTCGCAGCAG 
ATTCTATACA CACGCCATCC GGAACCAGAG AGTCACTACC GTCTGGAGAA TCCAATGATC 
CTCAAGGTGG TGGCCACCCT GATCCGGCAA TCAAAGCAGA CGGAATCGCT GATCGACGTT 
AAAAAGCTAT TCCTGCAGGA TATGACCCTG TTGTGCAATA GCAATCGGGA GAACCGGCGC 
ACAGTGCTCC AGATGTCCGT GTGGCAGGAG TGGCTCATTG CGATGGCGTA CATTCATCCA 
AAGAGCAGCG AGGAGCAGAA GATAAGCGAC ATGGTGTATT CCCTATTCCG CATGCTGCTC 
CACCATGCCA TTAAGCATGA ATACGGTGGC TGGAGAGTTT GGGTAGATAC CCTGGCAATC 
GTCCACTCCA AGGTGTCTTA CGAGGAGTTT AAGCTCCAGT TTGCCCAGAT GTACGAGCAC 
TACGAGCGCC AGCGAACGGA CAATATCACA GATCCTGCCC TGCGTCAGGC TAGACCCATC 
AGCACGATCA GTGGTTGGGA GCGAGAGGAG CTGCATCAGC AGCAGAACGG TGGATCTGCA 
GCAGCAGTGG CTACCAATCA AACGGCAGCT GTTAAAGGTT CTGTTTCCAT TGCCTCACTG 
GAAGATGTGC CACCGGTGGT TGAGGAAGAG GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG 
ATTCAAGAGG GTCCTATAAC CGAAGAAACC GAACAAAAAT CGGTGATAGC CAACATTTCC 
GACGTCTACA ATGAGCAGCT CAAAACCGAT GCCACGTGCA ATGGCAATTT GGAGGATGTC 
AAAGAGGAGG AGCCTGTACA ACAGCAAATC GGAGACCTGG AGAAGCAACC AGAGCCATCT 
ACTCCTTTGG GAGCACTCAG GGAAACCCTA CAACTTGGTG ATGATATGGA TGTGGAAGAA 
CTGGAGCTGG CTACCGCCAA GGATGCCCTC AATGCGGAAC AGCATGTGTC GCGAGTTCTT 
CAGGCTTCCG AAGCGGCACT TAACGATTGC AAAATGGCAG TCGATGATGT CCTGCAGGAG 
TCATCCTCCG TCCTCAAGGA CGAGGAGATC GAACTGGCCG TCAACGAGGT TGTTCAGGGT 
GTGCTCAATA ACGAGAAGAA AACTCAATCC CAGGACAACA AGGATAACAA GGAACAGCCG 
GGCGAACAGG ATGTAAACGT CAGTCTCCTA AACAGCAAGA ATCTGCTCAA CAATAATAAT 
AATAACAATA ACAACAGTCC GAGTCCAACG CCCACAACGG CGACTGCGAC GGCGGAGACG 
GAAGCGGAAA CAGAAGTCAA TGCCAATGAG ATCGTGAGCA GCACAGAGGC GCCCAAGGCG 
GAAACGGAAA CGAGCGTAGC ACCGGAAGTA GAAACACCGG AAACGGCCAA GCCAAGCCCC 
ATTGTCCCCA GCCCCGTATT AGCAACCAAT CAAAAGACTG AAGATGCAGC CAACAAGCTG 
AACAACAACG AGAAGCTGGC CGAGATCAGC GCCAGTCCCG AGCCACCCAT TGTCGTGGAA 
ACGCCAGAAG CTGATCTTCT TCAGCTTTCC GATTCCGAAA CAAAACCGAA TAAGGAAACC 
GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT AGAGATATTG TCGAGCAACT CATCGACAAG 
GTCATCGATG CCACGGAAGC GGAATCGGCC AGCGAAACCA AAACGGAAAC GAATAATAAT 
GAAATACCCA AGAAGGAGAA GCAGACGTCA GAAGAGCCTG AGGATGTGGA AACTGCAGAG 
ACTTTGGCCG CTGCCGCCAA GGAAATTGTC CAAGAGGTTG TGGAAGCAGC TCTCGTAATG 
GTCCAAGAGG AGAGCACTCA AGAGAAGCCA GAAAAGGGGG CGAACTCCGA AGAGGAGAAA 
AACGAAATTG GCAAGGAAGA GATTCTTCTG CAGCTGGAGG AAAAACCAGC GTCTACTGAA 
GTGCAGGAAA CCAAGATCGA AGGAGATCTA AAGAAACCAG AAGATCCAAA AGGTCACAGC 
TCTGTAGAAC CAAAGACCCC GAACCTTGAG GAACCGAAAC CCCAGGAAAC TGAACAACAA 
AAGTCGCAGG AGGTCGCCGA AGAACTACCT CAAAAACCAG AGGAGCAGGT GGTGGCCATT 
GTCAACCAAG TCCTGGACAC TCTGGTAGAC GATACGGTCA AGGCAGTGGC AGCCGAGCAA 
ACCACTCAAA CATCGCCAGC CCCCGAAGAG CAATCGCCAC AAATACTGGC AATGGAATCA 
CCAGCAACGT CGGTGCGGGT CAAACCCACT GAGGTGGATT CCACCACCCA GACTACGCCG 
AAGAACGAGG CTGGATCCAG TCTATTGGTT GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT 
GACGCCCAGC AATCAGCCGG CATGACCATC GAAGATGAGG ATTACTCTAA TCAGCAGGCA 
GCTGCGGCAG TTGAAAATGC CAACAGCAGC CAATTGGATG CCAATCATTA TGGTCCCGGT 
AATCCAGAAT CCAAACAACA GCAACAGCGC TCCAAATCGG GATCGACCCG ACCTATGTTC 
AGTCCGGGAC CTACACGTCC GCCCTTCCGA ATACCGGAAT TCAAGTGGTC CTACATCCAT 
CAGCGATTGC TCAGCGATGT CCTCTTTTCG CTGGAAACGG ACATTCAGGT GTGGCGCAGT 
CATTCGACCA AAAGCGTCCT GGACTTTGTC AACTCCAGCG AGAATGCAAT TTTTGTGGTG 
AACACGGTGC ATTTAATATC GCAGCTAGCG GATAATCTGA TTATTGCCTG CGGAGGATTG 
CTGCCTCTTT TGGCCAGCGC CACGTCACCC AATTCCGAAT TGGATGTGCT GGAGCCAACG 
CAAGGCATGC CTTTGGAGGT GGCCGTATCT TTCCTGCAGC GTCTGGTCAA CATGGCGGAT 
GTCCTGATCT TTGCCACATC CCTAAACTTC GGCGAGTTGG AGGCGGAGAA GAACATGTCC 
AGTGGCGGCA TACTGCGTCA GTGCCTGCGA TTGGTTTGCA CCTGTGCGGT AAGGAATTGC 
TTGGAGTGCA AGGAGCGAAC GCGTTACAAT GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT 
GCAGCCCATT TGCAGGCCCT CATCCGCGGA GCTCAAGCAT CGCCCAAGAA CATTGTCGAG 
TCAATAACCG GCCAATTATC GCCTGTCAAG GATCCCGAGA AGCTGCTCCA GGACATGGAC 
GTCAATCGCC TGCGCGCAGT CATCTATCGC GATGTGGAGG AGACTAAGCA GGCGCAGTTC 
CTCTCGCTGG CGATAGTGTA CTTCATATCG GTGCTGATGG TGTCCAAGTA TCGTGATATC 
CTGGAGCCGC CGGCAGAGCC GCAAATCCAG CGTCAATCGC CAGTGCTGCA ACGCACGGCA 
GGCGGCGAAG CCGCCAGTGC GCGTCCTTTG TTCCCCCAAT GGTCGCATCA TGTTTACCCG 
CAATTCCTGC CCGAATCGCA CCAGAATCAC AACAGCAACA TGCAACACCA GCAGCAGCAG 
CAGCAGCAAC AGCAACATCA GCAGCAGCAA CACTACTACC AGCAACAGCA GCAACAGCAG 
GTTGTCCACA ATCATAGCCA TCATCACATG ACTGCCGCTT ACTACCAACA GCAGCAACAC 
CAGCAAGTTG CAGCAGGACA GCAGCAACAT TCGCCCACTC CTCTGGCCAC ACATTCGACC 
AGCTCCTCGG CCAGTTCGAC GGCCACATCT CAGCCAGCGT CGAGCTCCTC GCTCTCCAGC 
CTGGCATCCC AAAGTCAGCA GCAATCGCAT CGCCAGTTGC ACAAGCAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAACA ACAGCCACAT TACCATCCAC ATCAGCCGCA CTATGGACTG 
ATCAATGGGC ACCAGCAACA TCCGCAGCTA AATGGTAAAC ATTATGCGGA AAATGGATCG 
ACAGCGGGAT ACCATCCGCA TTCGCATCCA CATCCACACG GTGGTTATAT GCGAAATGGT 
GATACGTCGA CATTGCAACA GAATGGTATG CCGGACTATC AGGCAGTGGG TCTGATGAAT 
GGTCATGGCT CTGGTGGAAC GGGTAATAAC AATAACTCCA GCCTGATGAA CAACATGCGA 
AATCTGCGAA ATGGCGGAGG AGCTAACTCA TCATCTTCGC CACCGGGAAG CCATCAAGTT 
ATCCAAGGTG TTGCAACAGC GGGAGCTGTT AATGCAAATG CTGCTGTTGG TGTTGGCATG 
GGCGGAGCCG TTGGTGTGGG TATGGGGGGT GTGGCAGGTG GTTTGGATGG AGGCGTGGTC 
TACAAGACGA GCGCCATAAA TAATAATTAC CGCTACAATG GACGCAACGC ATCCGCTGGA 
ACAGGTGGTC GTCAAATCCA GGATAGTGAC TACGAAATAA TTGTCGTCGA TGAGAACAAT 
CCATCGGTTT TGGCGGATAA TGATTCACAT TCCAGTGGAC CACCATCCAT AAAGGCCAAC 
CAGACAACAA CAATAGCAAC AACAACTACT ACAACAGCAA CAACCCATAT TAACAACAAC 
AACACTAAGA CTACAACATC AAACGCTCCA ACAGCAACAA TTAAAATTCA GCAACAACCA 
TTGTCACCAC CAAAGCCGTT GGTGCCACAA AAATTGCCAA AGAGCGTTGA CTCGGATGTG 
GGCTCCCTGA ACATGAACTC CACGGAAAAC GAAGTGCCCG AGGTGGAGTC ATCCAGCGAG 
ATTCTAATCG ATGACCACAA ACCGAGTCAC TCGAACGACG AAAGCTGGAC GGATGTGAAT 
CTCAATGAGG ATGCTGCCGT TCAGGCGGCA AGTGCCGGAA TAGTTGTAGG TTTGGTGGAT 
AACAGAGGTA ATGTTATATC CGACAAACAC GATCCGTCCC ATCACAACCA ACAACAGCAG 
CAGCAGGCGG GAATTATTGG TCAGCAGCAG CAACATGGAT CACTTGGTCA TTCGGAGCGG 
GGTGACAAAC CCGATTCGGA GATTTCGGTG GTGCGAGTGC CTGATGGTTA TGGTGGTGCT 
GGTTCCGGTG GTGGCGTGAA TTCCGGACAA GGTCAGGGCG TTCCATCCAA TCAGCGTCCT 
CGTCCCGAGG AGTTGCCCAT GAAGGCGCCC GCCTTGGTGG CCCAACTGCC GCTGACCACA 
CCTTCGCGAG AGGCCAGTCT CACCCAGAAG CTGGAAATCG CATTGGGGCC AGTGTGTCCG 
CTGCTCCGTG AAATCATGGT GGACTTTGCG CCGTTCCTTT CGAAAACCCT AGTCGGCTCC 
CATGGCCAGG AATTGCTGAT GGAGGGCAAG GGTCTGACGA CCTTTAAGAA CTCGCATTCC 
GTCGTAGAGC TGGTCATGTT GCTCTGCTCC CAGGAGTGGC AAAATAGCCT ACAAAAGCAC 
GCAGGATTAG CCTTTATTGA GCTAATCAAT GAGGGTCGAC TGCTTTCGCA CGCCATGAAG 
GATCACATTG TTCGGGTGGC CAATGAGGCG GAGTTCATCC TCAATAGAAT GCGAGCGGAT 
GATGTCCTCA AGCATGCCGA CTTTGAATCG CAATGTGCAC AGACACTTTT GGAGCGCAGG 
GAGGAGGAGC GAATGTGCGA TCACTTGATA ACCGCCGCAC GTCGCAGAGA CAATGTGATT 
GCCAGCCGAC TGCTGGAGAA GGTACGAAAC ATCATGTGTA ATCGCCACGG AGCCTGGGGT 
GATTCCAGCT CCACATCCAG TGGTGGCGCC ATCGTTGGAG CGGTGCAAAA GAGTCCCTAC 
TGGAAGCTGG ATGCCTGGGA GGATGATGCC AGACGTCGGA AACGGATGGT GCAAAATCCG 
CGCGGATCCT CGCATCCACA GGCCACACTG AAGGCGGCTT TGGAAAATGG CGGACCTGAA 
GATGCCATCC TGCAGACTCG CGACGAGTTC CACACCCAAA TAGCCGTCTC GCGGACTCAT 
CCATCCGGTC AGCACAATGG TGAGCTACTG GACGACGCCG AGCTCTTGAT TGAGGATCGG 
GAGTTGGATC TGGATCTCAC GGGTCCCGTC AACATTAGCA CCAAGGCGAG ATTGATAGCC 
CCCGGTCTGG TGGCACCCGG CACCGTCTCA ATAACCAGCA CTGAAATGTT CTTTGAGGTG 
GATGAAGAGC ATCCCGAGTT CCAGAAGATC GATGGCGAAG TTCTTAAGTA CTGTGACCAT 
TTGCACGGCA AATGGTACTT CTCCGAAGTG AGGGCCATCT TCTCGAGGCG TTATCTCCTG 
CAAAATGTGG CCCTCGAGAT CTTTTTGGCC AGCCGTACGT CCATATTGTT TGCCTTTCCC 
GATCAGCATA CGGTCAAGAA GGTAATCAAG GCCCTGCCCC GTGTGGGAGT GGGCATCAAG 
TATGGAATAC CGCAGACGAG GAGGGCCTCA ATGATGTCGC CCCGTCAACT GATGCGCAAT 
TCGAACATGA CCCAAAAGTG GCAGCGTCGC GAGATTAGCA ACTTTGAGTA TCTTATGTTC 
CTGAACACGA TCGCCGGCAG GACGTACAAC GATCTAAATC AGTATCCCAT CTTTCCGTGG 
GTGCTGACCA ACTATGAGTC CAAGGATTTG GATCTCAGCC TGCCGTCAAA CTACAGGGAT 
CTGTCGAAAC CGATTGGCGC CCTGAATCCA TCGCGCAGAG CCTATTTCGA GGAGCGTTAC 
GAGAGCTGGG ACAGTGACAC CATACCGCCC TTCCACTATG GTACGCATTA TTCCACGGCA 
GCATTTACGC TGAACTGGTT GGTGCGCGTG GAACCGTTTA CCACCATGTT CCTGGCCCTG 
CAGGGCGGCA AATTTGATTA TCCGGACAGG CTGTTCAGCT CGGTGTCGCT GTCCTGGAAG 
AATTGCCAAC GTGATACATC GGATGTGAAG GAACTGATAC CAGAATGGTA TTTCCTGCCC 
GAAATGTTTT ACAATTCATC AGGCTATCGA CTGGGTCATC GCGAGGATGG TGCCCTAGTG 
GATGACATTG AACTACCACC GTGGGCCAAG AGCCCCGAAG AATTCGTCCG CATCAATCGC 
ATGGCACTGG AGTCGGAGTT CGTGTCCTGC CAGCTGCACC AGTGGATCGA TTTGATCTTT 
GGCTACAAGC AACGCGGTCC CGAGGCCATT AGGGCAACCA ACGTGTTCTA CTATTTGACA 
TATGAGGGCA GCGTTGACTT GGATGGCGTT TTGGATCCGG TGATGCGCGA AGCCGTCGAG 
AATCAAATCC GCAACTTTGG CCAAACACCT AGTCAACTGC TAATGGAACC GCATCCGCCG 
CGCAGCTCTG CGATGCATCT ATCGCCTATG ATGTTCAGTG CGATGCCGGA GGATCTCTGC 
CAGATGCTCA AGTTCTATCA GAACTCACCA GTCATTCACA TCTCGGCCAA CACCTATCCG 
CAACTGTCGT TGCCATCTGT GGTCACGGTA ACGGCGGGTC ATCAGTTTGC GGTGAATCGA 
TGGAACTGCA ACTACACCGC CTCCGTCCAG AGTCCCAGCT ACGCCGAATC GCCCCAATCG 
CCGGGTTCCA ATCAACCACT GACCATCGAT CCGGTTCTGG CTGTTCATGG CACCAACAAC 
AATAGCAATG CGGCCAGCCG ACGTCATTTG GGCGATAACT TTAGCCAGAT GCTAAAGATA 
CGCTCCAACT GCTTTGTTAC CACAGTGGAC AGTCGGTTCC TAATCGCATG CGGATTCTGG 
GACAACAGTT TCCGCGTCTT TGCCACCGAA ACGGCGAAAA TCGTGCAGAT TGTATTCGGT 
CACTTTGGTG TGGTAACGTG CATGGCACGT TCCGAGTGCA ACATCACATC AGACTGCTAC 
ATCGCCTCCG GATCGGCGGA CTGCACGGTG CTTCTGTGGC ACTGGAATGC CCGAACCCAG 
AGCATTGTGG GCGAGGGCGA TGTGCCCACA CCACGGGCCA CCTTGACGGG TCACGAACAG 
GCCGTGACAT CGGTGGTGAT TAGTGCCGAG TTGGGTCTGG TTGTGTCTGG ATCATCAAAT 
GGACCCGTAC TTATCCATAC GACTTTTGGC GACCTGCTGC GCTCACTGGA TCCACCAGCG 
GAGTTCCATT CCCCGGAACT GATAACCATG TCCCGCGAGG GTTTCATTGT CATCAACTAC 
GATAAGGGCA ATGTGGCCGC CTACACCATC AATGGCAAGA AACTGCGCCA CGAGACGCAC 
AACGACAATC TACAGTGCAT GCTACTGTCG CGCGATGGTG AATACCTGAT GACCGCCGGT 
GATCGCGGCA TCGTGGAGGT GTGGCGCACC TTCAACCTAG CACCACTTTA TGCCTTCCCC 
GCATGCAACG CGGGCATCCG ATCTTTGGCT CTCACCCACG ATCAGAAATA CCTTCTGGCC 
GGACTTTCGA CGGGCTCGAT CATAGTATTC CACATCGACT TCAATCGCTG GCACCACGAG 
TACCAGCAGC GCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rg ( NM_001144686.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138158.2 Drosophila melanogaster rugose (rg), transcript variant N, mRNA.

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CloneID	OFa22216
Clone ID Related Accession (Same CDS sequence)	NM_001144686.2
Accession Version	NM_001144686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform N
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144686.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGTTG 
TTCTGTTTCT CGTTTCTCAC GTGGCAGCTG CTGGATCACG TCCTCTTCAA TCCAGCCCTG 
TGGATATACA CACCCGCCAA TGTCCAGGCG CGACTATACT CCTACTTGGC CACCGAGTTC 
CTCTCGGACA CGCAGATCTA CAGCAATGTG AGGAGGGTCA GCACGGTACT GCAGACAGTG 
CACACCCTAA AGTACTACTA CTGGGTGGTC AATCCACGGG CTAAGAGCGG CATCATTCCC 
AAGGGACTGG ATGGACCTCG TCCGGCTCAA AAGGACATCC TGGCCATCCG GGCATACATC 
CTGCTGTTCC TGAAGCAGCT CATAATGATC GGCAATGGCG TGAAGGAGGA CGAGCTGCAG 
AGCATACTCA ACTATCTGAC CACCATGCAC GAGGACGAGA ACCTGCACGA CGTGCTGCAG 
ATGCTCATCT CGCTGATGTC GGAGCATCCC AGCTCCATGG TACCTGCCTT TGATGTGAAG 
CACGGCGTGC GCAGCATCTT CAAGTTGCTG GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG 
GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC CGCAGCACCC ACAAACGCAA GTACGATGTG 
ATGTCACCAC ACAATCTGTA CACCCTTTTA GCGGAGCGAC TGCTTCTCTA TGAGGAATCA 
CTGTCCCTGC CCACATACAA TGTTCTCTAT GAGATCATGA CGGAGCACAT CTCGCAGCAG 
ATTCTATACA CACGCCATCC GGAACCAGAG AGTCACTACC GTCTGGAGAA TCCAATGATC 
CTCAAGGTGG TGGCCACCCT GATCCGGCAA TCAAAGCAGA CGGAATCGCT GATCGACGTT 
AAAAAGCTAT TCCTGCAGGA TATGACCCTG TTGTGCAATA GCAATCGGGA GAACCGGCGC 
ACAGTGCTCC AGATGTCCGT GTGGCAGGAG TGGCTCATTG CGATGGCGTA CATTCATCCA 
AAGAGCAGCG AGGAGCAGAA GATAAGCGAC ATGGTGTATT CCCTATTCCG CATGCTGCTC 
CACCATGCCA TTAAGCATGA ATACGGTGGC TGGAGAGTTT GGGTAGATAC CCTGGCAATC 
GTCCACTCCA AGGTGTCTTA CGAGGAGTTT AAGCTCCAGT TTGCCCAGAT GTACGAGCAC 
TACGAGCGCC AGCGAACGGA CAATATCACA GATCCTGCCC TGCGTCAGGC TAGACCCATC 
AGCACGATCA GTGGTTGGGA GCGAGAGGAG CTGCATCAGC AGCAGAACGG TGGATCTGCA 
GCAGCAGTGG CTACCAATCA AACGGCAGCT GTTAAAGGTT CTGTTTCCAT TGCCTCACTG 
GAAGATGTGC CACCGGTGGT TGAGGAAGAG GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG 
ATTCAAGAGG GTCCTATAAC CGAAGAAACC GAACAAAAAT CGGTGATAGC CAACATTTCC 
GACGTCTACA ATGAGCAGCT CAAAACCGAT GCCACGTGCA ATGGCAATTT GGAGGATGTC 
AAAGAGGAGG AGCCTGTACA ACAGCAAATC GGAGACCTGG AGAAGCAACC AGAGCCATCT 
ACTCCTTTGG GAGCACTCAG GGAAACCCTA CAACTTGGTG ATGATATGGA TGTGGAAGAA 
CTGGAGCTGG CTACCGCCAA GGATGCCCTC AATGCGGAAC AGCATGTGTC GCGAGTTCTT 
CAGGCTTCCG AAGCGGCACT TAACGATTGC AAAATGGCAG TCGATGATGT CCTGCAGGAG 
TCATCCTCCG TCCTCAAGGA CGAGGAGATC GAACTGGCCG TCAACGAGGT TGTTCAGGGT 
GTGCTCAATA ACGAGAAGAA AACTCAATCC CAGGACAACA AGGATAACAA GGAACAGCCG 
GGCGAACAGG ATGTAAACGT CAGTCTCCTA AACAGCAAGA ATCTGCTCAA CAATAATAAT 
AATAACAATA ACAACAGTCC GAGTCCAACG CCCACAACGG CGACTGCGAC GGCGGAGACG 
GAAGCGGAAA CAGAAGTCAA TGCCAATGAG ATCGTGAGCA GCACAGAGGC GCCCAAGGCG 
GAAACGGAAA CGAGCGTAGC ACCGGAAGTA GAAACACCGG AAACGGCCAA GCCAAGCCCC 
ATTGTCCCCA GCCCCGTATT AGCAACCAAT CAAAAGACTG AAGATGCAGC CAACAAGCTG 
AACAACAACG AGAAGCTGGC CGAGATCAGC GCCAGTCCCG AGCCACCCAT TGTCGTGGAA 
ACGCCAGAAG CTGATCTTCT TCAGCTTTCC GATTCCGAAA CAAAACCGAA TAAGGAAACC 
GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT AGAGATATTG TCGAGCAACT CATCGACAAG 
GTCATCGATG CCACGGAAGC GGAATCGGCC AGCGAAACCA AAACGGAAAC GAATAATAAT 
GAAATACCCA AGAAGGAGAA GCAGACGTCA GAAGAGCCTG AGGATGTGGA AACTGCAGAG 
ACTTTGGCCG CTGCCGCCAA GGAAATTGTC CAAGAGGTTG TGGAAGCAGC TCTCGTAATG 
GTCCAAGAGG AGAGCACTCA AGAGAAGCCA GAAAAGGGGG CGAACTCCGA AGAGGAGAAA 
AACGAAATTG GCAAGGAAGA GATTCTTCTG CAGCTGGAGG AAAAACCAGC GTCTACTGAA 
GTGCAGGAAA CCAAGATCGA AGGAGATCTA AAGAAACCAG AAGATCCAAA AGGTCACAGC 
TCTGTAGAAC CAAAGACCCC GAACCTTGAG GAACCGAAAC CCCAGGAAAC TGAACAACAA 
AAGTCGCAGG AGGTCGCCGA AGAACTACCT CAAAAACCAG AGGAGCAGGT GGTGGCCATT 
GTCAACCAAG TCCTGGACAC TCTGGTAGAC GATACGGTCA AGGCAGTGGC AGCCGAGCAA 
ACCACTCAAA CATCGCCAGC CCCCGAAGAG CAATCGCCAC AAATACTGGC AATGGAATCA 
CCAGCAACGT CGGTGCGGGT CAAACCCACT GAGGTGGATT CCACCACCCA GACTACGCCG 
AAGAACGAGG CTGGATCCAG TCTATTGGTT GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT 
GACGCCCAGC AATCAGCCGG CATGACCATC GAAGATGAGG ATTACTCTAA TCAGCAGGCA 
GCTGCGGCAG TTGAAAATGC CAACAGCAGC CAATTGGATG CCAATCATTA TGGTCCCGGT 
AATCCAGAAT CCAAACAACA GCAACAGCGC TCCAAATCGG GATCGACCCG ACCTATGTTC 
AGTCCGGGAC CTACACGTCC GCCCTTCCGA ATACCGGAAT TCAAGTGGTC CTACATCCAT 
CAGCGATTGC TCAGCGATGT CCTCTTTTCG CTGGAAACGG ACATTCAGGT GTGGCGCAGT 
CATTCGACCA AAAGCGTCCT GGACTTTGTC AACTCCAGCG AGAATGCAAT TTTTGTGGTG 
AACACGGTGC ATTTAATATC GCAGCTAGCG GATAATCTGA TTATTGCCTG CGGAGGATTG 
CTGCCTCTTT TGGCCAGCGC CACGTCACCC AATTCCGAAT TGGATGTGCT GGAGCCAACG 
CAAGGCATGC CTTTGGAGGT GGCCGTATCT TTCCTGCAGC GTCTGGTCAA CATGGCGGAT 
GTCCTGATCT TTGCCACATC CCTAAACTTC GGCGAGTTGG AGGCGGAGAA GAACATGTCC 
AGTGGCGGCA TACTGCGTCA GTGCCTGCGA TTGGTTTGCA CCTGTGCGGT AAGGAATTGC 
TTGGAGTGCA AGGAGCGAAC GCGTTACAAT GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT 
GCAGCCCATT TGCAGGCCCT CATCCGCGGA GCTCAAGCAT CGCCCAAGAA CATTGTCGAG 
TCAATAACCG GCCAATTATC GCCTGTCAAG GATCCCGAGA AGCTGCTCCA GGACATGGAC 
GTCAATCGCC TGCGCGCAGT CATCTATCGC GATGTGGAGG AGACTAAGCA GGCGCAGTTC 
CTCTCGCTGG CGATAGTGTA CTTCATATCG GTGCTGATGG TGTCCAAGTA TCGTGATATC 
CTGGAGCCGC CGGCAGAGCC GCAAATCCAG CGTCAATCGC CAGTGCTGCA ACGCACGGCA 
GGCGGCGGTG GTCGTCAAAT CCAGGATAGT GACTACGAAA TAATTGTCGT CGATGAGAAC 
AATCCATCGG TTTTGGCGGA TAATGATTCA CATTCCAGTG GACCACCATC CATAAAGGCC 
AACCAGACAA CAACAATAGC AACAACAACT ACTACAACAG CAACAACCCA TATTAACAAC 
AACAACACTA AGACTACAAC ATCAAACGCT CCAACAGCAA CAATTAAAAT TCAGCAACAA 
CCATTGTCAC CACCAAAGCC GTTGGTGCCA CAAAAATTGC CAAAGAGCGT TGACTCGGAT 
GTGGGCTCCC TGAACATGAA CTCCACGGAA AACGAAGTGC CCGAGGTGGA GTCATCCAGC 
GAGATTCTAA TCGATGACCA CAAACCGAGT CACTCGAACG ACGAAAGCTG GACGGATGTG 
AATCTCAATG AGGATGCTGC CGTTCAGGCG GCAAGTGCCG GAATAGTTGT AGGTTTGGTG 
GATAACAGAG GTAATGTTAT ATCCGACAAA CACGATCCGT CCCATCACAA CCAACAACAG 
CAGCAGCAGG CGGGAATTAT TGGTCAGCAG CAGCAACATG GATCACTTGG TCATTCGGAG 
CGGGGTGACA AACCCGATTC GGAGATTTCG GTGGTGCGAG TGCCTGATGG TTATGGTGGT 
GCTGGTTCCG GTGGTGGCGT GAATTCCGGA CAAGGTCAGG GCGTTCCATC CAATCAGCGT 
CCTCGTCCCG AGGAGTTGCC CATGAAGGCG CCCGCCTTGG TGGCCCAACT GCCGCTGACC 
ACACCTTCGC GAGAGGCCAG TCTCACCCAG AAGCTGGAAA TCGCATTGGG GCCAGTGTGT 
CCGCTGCTCC GTGAAATCAT GGTGGACTTT GCGCCGTTCC TTTCGAAAAC CCTAGTCGGC 
TCCCATGGCC AGGAATTGCT GATGGAGGGC AAGGGTCTGA CGACCTTTAA GAACTCGCAT 
TCCGTCGTAG AGCTGGTCAT GTTGCTCTGC TCCCAGGAGT GGCAAAATAG CCTACAAAAG 
CACGCAGGAT TAGCCTTTAT TGAGCTAATC AATGAGGGTC GACTGCTTTC GCACGCCATG 
AAGGATCACA TTGTTCGGGT GGCCAATGAG GCGGAGTTCA TCCTCAATAG AATGCGAGCG 
GATGATGTCC TCAAGCATGC CGACTTTGAA TCGCAATGTG CACAGACACT TTTGGAGCGC 
AGGGAGGAGG AGCGAATGTG CGATCACTTG ATAACCGCCG CACGTCGCAG AGACAATGTG 
ATTGCCAGCC GACTGCTGGA GAAGGTACGA AACATCATGT GTAATCGCCA CGGAGCCTGG 
GGTGATTCCA GCTCCACATC CAGTGGTGGC GCCATCGTTG GAGCGGTGCA AAAGAGTCCC 
TACTGGAAGC TGGATGCCTG GGAGGATGAT GCCAGACGTC GGAAACGGAT GGTGCAAAAT 
CCGCGCGGAT CCTCGCATCC ACAGGCCACA CTGAAGGCGG CTTTGGAAAA TGGCGGACCT 
GAAGATGCCA TCCTGCAGAC TCGCGACGAG TTCCACACCC AAATAGCCGT CTCGCGGACT 
CATCCATCCG GTCAGCACAA TGGTGAGCTA CTGGACGACG CCGAGCTCTT GATTGAGGAT 
CGGGAGTTGG ATCTGGATCT CACGGGTCCC GTCAACATTA GCACCAAGGC GAGATTGATA 
GCCCCCGGTC TGGTGGCACC CGGCACCGTC TCAATAACCA GCACTGAAAT GTTCTTTGAG 
GTGGATGAAG AGCATCCCGA GTTCCAGAAG ATCGATGGCG AAGTTCTTAA GTACTGTGAC 
CATTTGCACG GCAAATGGTA CTTCTCCGAA GTGAGGGCCA TCTTCTCGAG GCGTTATCTC 
CTGCAAAATG TGGCCCTCGA GATCTTTTTG GCCAGCCGTA CGTCCATATT GTTTGCCTTT 
CCCGATCAGC ATACGGTCAA GAAGGTAATC AAGGCCCTGC CCCGTGTGGG AGTGGGCATC 
AAGTATGGAA TACCGCAGAC GAGGAGGGCC TCAATGATGT CGCCCCGTCA ACTGATGCGC 
AATTCGAACA TGACCCAAAA GTGGCAGCGT CGCGAGATTA GCAACTTTGA GTATCTTATG 
TTCCTGAACA CGATCGCCGG CAGGACGTAC AACGATCTAA ATCAGTATCC CATCTTTCCG 
TGGGTGCTGA CCAACTATGA GTCCAAGGAT TTGGATCTCA GCCTGCCGTC AAACTACAGG 
GATCTGTCGA AACCGATTGG CGCCCTGAAT CCATCGCGCA GAGCCTATTT CGAGGAGCGT 
TACGAGAGCT GGGACAGTGA CACCATACCG CCCTTCCACT ATGGTACGCA TTATTCCACG 
GCAGCATTTA CGCTGAACTG GTTGGTGCGC GTGGAACCGT TTACCACCAT GTTCCTGGCC 
CTGCAGGGCG GCAAATTTGA TTATCCGGAC AGGCTGTTCA GCTCGGTGTC GCTGTCCTGG 
AAGAATTGCC AACGTGATAC ATCGGATGTG AAGGAACTGA TACCAGAATG GTATTTCCTG 
CCCGAAATGT TTTACAATTC ATCAGGCTAT CGACTGGGTC ATCGCGAGGA TGGTGCCCTA 
GTGGATGACA TTGAACTACC ACCGTGGGCC AAGAGCCCCG AAGAATTCGT CCGCATCAAT 
CGCATGGCAC TGGAGTCGGA GTTCGTGTCC TGCCAGCTGC ACCAGTGGAT CGATTTGATC 
TTTGGCTACA AGCAACGCGG TCCCGAGGCC ATTAGGGCAA CCAACGTGTT CTACTATTTG 
ACATATGAGG GCAGCGTTGA CTTGGATGGC GTTTTGGATC CGGTGATGCG CGAAGCCGTC 
GAGAATCAAA TCCGCAACTT TGGCCAAACA CCTAGTCAAC TGCTAATGGA ACCGCATCCG 
CCGCGCAGCT CTGCGATGCA TCTATCGCCT ATGATGTTCA GTGCGATGCC GGAGGATCTC 
TGCCAGATGC TCAAGTTCTA TCAGAACTCA CCAGTCATTC ACATCTCGGC CAACACCTAT 
CCGCAACTGT CGTTGCCATC TGTGGTCACG GTAACGGCGG GTCATCAGTT TGCGGTGAAT 
CGATGGAACT GCAACTACAC CGCCTCCGTC CAGAGTCCCA GCTACGCCGA ATCGCCCCAA 
TCGCCGGGTT CCAATCAACC ACTGACCATC GATCCGGTTC TGGCTGTTCA TGGCACCAAC 
AACAATAGCA ATGCGGCCAG CCGACGTCAT TTGGGCGATA ACTTTAGCCA GATGCTAAAG 
ATACGCTCCA ACTGCTTTGT TACCACAGTG GACAGTCGGT TCCTAATCGC ATGCGGATTC 
TGGGACAACA GTTTCCGCGT CTTTGCCACC GAAACGGCGA AAATCGTGCA GATTGTATTC 
GGTCACTTTG GTGTGGTAAC GTGCATGGCA CGTTCCGAGT GCAACATCAC ATCAGACTGC 
TACATCGCCT CCGGATCGGC GGACTGCACG GTGCTTCTGT GGCACTGGAA TGCCCGAACC 
CAGAGCATTG TGGGCGAGGG CGATGTGCCC ACACCACGGG CCACCTTGAC GGGTCACGAA 
CAGGCCGTGA CATCGGTGGT GATTAGTGCC GAGTTGGGTC TGGTTGTGTC TGGATCATCA 
AATGGACCCG TACTTATCCA TACGACTTTT GGCGACCTGC TGCGCTCACT GGATCCACCA 
GCGGAGTTCC ATTCCCCGGA ACTGATAACC ATGTCCCGCG AGGGTTTCAT TGTCATCAAC 
TACGATAAGG GCAATGTGGC CGCCTACACC ATCAATGGCA AGAAACTGCG CCACGAGACG 
CACAACGACA ATCTACAGTG CATGCTACTG TCGCGCGATG GTGAATACCT GATGACCGCC 
GGTGATCGCG GCATCGTGGA GGTGTGGCGC ACCTTCAACC TAGCACCACT TTATGCCTTC 
CCCGCATGCA ACGCGGGCAT CCGATCTTTG GCTCTCACCC ACGATCAGAA ATACCTTCTG 
GCCGGACTTT CGACGGGCTC GATCATAGTA TTCCACATCG ACTTCAATCG CTGGCACCAC 
GAGTACCAGC AGCGCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138158.2
CDS	1089..11489
Translation	MADIMRPPYSEIKRPDEIVRMTTADNLKFAVLIGLIEVGQVTNREVVNTVLHLLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSHFQLLPATLGASALSGGSGSGTGSGTGNDASAAAAAAVAAGQQQQLQLQFQILAAEQEARAIDWSDEKLDLNAAFVKIRAVLTARNAVTLAGSSSTGTTAVATAAAAAAAAGAGAGTTAAATSAAAAAAATQNENDAAVGQQQHATHHHATAATGSADDPLGHLPTGNASSSSSSFEQLRRMSSVSSLNSMVGSADTEEVNQLKAVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLFCFSFLTWQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKANQTTTIATTTTTTATTHINNNNTKTTTSNAPTATIKIQQQPLSPPKPLVPQKLPKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_001144686.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant N, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144686.2

ORF Insert Sequence:

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGTTG 
TTCTGTTTCT CGTTTCTCAC GTGGCAGCTG CTGGATCACG TCCTCTTCAA TCCAGCCCTG 
TGGATATACA CACCCGCCAA TGTCCAGGCG CGACTATACT CCTACTTGGC CACCGAGTTC 
CTCTCGGACA CGCAGATCTA CAGCAATGTG AGGAGGGTCA GCACGGTACT GCAGACAGTG 
CACACCCTAA AGTACTACTA CTGGGTGGTC AATCCACGGG CTAAGAGCGG CATCATTCCC 
AAGGGACTGG ATGGACCTCG TCCGGCTCAA AAGGACATCC TGGCCATCCG GGCATACATC 
CTGCTGTTCC TGAAGCAGCT CATAATGATC GGCAATGGCG TGAAGGAGGA CGAGCTGCAG 
AGCATACTCA ACTATCTGAC CACCATGCAC GAGGACGAGA ACCTGCACGA CGTGCTGCAG 
ATGCTCATCT CGCTGATGTC GGAGCATCCC AGCTCCATGG TACCTGCCTT TGATGTGAAG 
CACGGCGTGC GCAGCATCTT CAAGTTGCTG GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG 
GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC CGCAGCACCC ACAAACGCAA GTACGATGTG 
ATGTCACCAC ACAATCTGTA CACCCTTTTA GCGGAGCGAC TGCTTCTCTA TGAGGAATCA 
CTGTCCCTGC CCACATACAA TGTTCTCTAT GAGATCATGA CGGAGCACAT CTCGCAGCAG 
ATTCTATACA CACGCCATCC GGAACCAGAG AGTCACTACC GTCTGGAGAA TCCAATGATC 
CTCAAGGTGG TGGCCACCCT GATCCGGCAA TCAAAGCAGA CGGAATCGCT GATCGACGTT 
AAAAAGCTAT TCCTGCAGGA TATGACCCTG TTGTGCAATA GCAATCGGGA GAACCGGCGC 
ACAGTGCTCC AGATGTCCGT GTGGCAGGAG TGGCTCATTG CGATGGCGTA CATTCATCCA 
AAGAGCAGCG AGGAGCAGAA GATAAGCGAC ATGGTGTATT CCCTATTCCG CATGCTGCTC 
CACCATGCCA TTAAGCATGA ATACGGTGGC TGGAGAGTTT GGGTAGATAC CCTGGCAATC 
GTCCACTCCA AGGTGTCTTA CGAGGAGTTT AAGCTCCAGT TTGCCCAGAT GTACGAGCAC 
TACGAGCGCC AGCGAACGGA CAATATCACA GATCCTGCCC TGCGTCAGGC TAGACCCATC 
AGCACGATCA GTGGTTGGGA GCGAGAGGAG CTGCATCAGC AGCAGAACGG TGGATCTGCA 
GCAGCAGTGG CTACCAATCA AACGGCAGCT GTTAAAGGTT CTGTTTCCAT TGCCTCACTG 
GAAGATGTGC CACCGGTGGT TGAGGAAGAG GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG 
ATTCAAGAGG GTCCTATAAC CGAAGAAACC GAACAAAAAT CGGTGATAGC CAACATTTCC 
GACGTCTACA ATGAGCAGCT CAAAACCGAT GCCACGTGCA ATGGCAATTT GGAGGATGTC 
AAAGAGGAGG AGCCTGTACA ACAGCAAATC GGAGACCTGG AGAAGCAACC AGAGCCATCT 
ACTCCTTTGG GAGCACTCAG GGAAACCCTA CAACTTGGTG ATGATATGGA TGTGGAAGAA 
CTGGAGCTGG CTACCGCCAA GGATGCCCTC AATGCGGAAC AGCATGTGTC GCGAGTTCTT 
CAGGCTTCCG AAGCGGCACT TAACGATTGC AAAATGGCAG TCGATGATGT CCTGCAGGAG 
TCATCCTCCG TCCTCAAGGA CGAGGAGATC GAACTGGCCG TCAACGAGGT TGTTCAGGGT 
GTGCTCAATA ACGAGAAGAA AACTCAATCC CAGGACAACA AGGATAACAA GGAACAGCCG 
GGCGAACAGG ATGTAAACGT CAGTCTCCTA AACAGCAAGA ATCTGCTCAA CAATAATAAT 
AATAACAATA ACAACAGTCC GAGTCCAACG CCCACAACGG CGACTGCGAC GGCGGAGACG 
GAAGCGGAAA CAGAAGTCAA TGCCAATGAG ATCGTGAGCA GCACAGAGGC GCCCAAGGCG 
GAAACGGAAA CGAGCGTAGC ACCGGAAGTA GAAACACCGG AAACGGCCAA GCCAAGCCCC 
ATTGTCCCCA GCCCCGTATT AGCAACCAAT CAAAAGACTG AAGATGCAGC CAACAAGCTG 
AACAACAACG AGAAGCTGGC CGAGATCAGC GCCAGTCCCG AGCCACCCAT TGTCGTGGAA 
ACGCCAGAAG CTGATCTTCT TCAGCTTTCC GATTCCGAAA CAAAACCGAA TAAGGAAACC 
GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT AGAGATATTG TCGAGCAACT CATCGACAAG 
GTCATCGATG CCACGGAAGC GGAATCGGCC AGCGAAACCA AAACGGAAAC GAATAATAAT 
GAAATACCCA AGAAGGAGAA GCAGACGTCA GAAGAGCCTG AGGATGTGGA AACTGCAGAG 
ACTTTGGCCG CTGCCGCCAA GGAAATTGTC CAAGAGGTTG TGGAAGCAGC TCTCGTAATG 
GTCCAAGAGG AGAGCACTCA AGAGAAGCCA GAAAAGGGGG CGAACTCCGA AGAGGAGAAA 
AACGAAATTG GCAAGGAAGA GATTCTTCTG CAGCTGGAGG AAAAACCAGC GTCTACTGAA 
GTGCAGGAAA CCAAGATCGA AGGAGATCTA AAGAAACCAG AAGATCCAAA AGGTCACAGC 
TCTGTAGAAC CAAAGACCCC GAACCTTGAG GAACCGAAAC CCCAGGAAAC TGAACAACAA 
AAGTCGCAGG AGGTCGCCGA AGAACTACCT CAAAAACCAG AGGAGCAGGT GGTGGCCATT 
GTCAACCAAG TCCTGGACAC TCTGGTAGAC GATACGGTCA AGGCAGTGGC AGCCGAGCAA 
ACCACTCAAA CATCGCCAGC CCCCGAAGAG CAATCGCCAC AAATACTGGC AATGGAATCA 
CCAGCAACGT CGGTGCGGGT CAAACCCACT GAGGTGGATT CCACCACCCA GACTACGCCG 
AAGAACGAGG CTGGATCCAG TCTATTGGTT GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT 
GACGCCCAGC AATCAGCCGG CATGACCATC GAAGATGAGG ATTACTCTAA TCAGCAGGCA 
GCTGCGGCAG TTGAAAATGC CAACAGCAGC CAATTGGATG CCAATCATTA TGGTCCCGGT 
AATCCAGAAT CCAAACAACA GCAACAGCGC TCCAAATCGG GATCGACCCG ACCTATGTTC 
AGTCCGGGAC CTACACGTCC GCCCTTCCGA ATACCGGAAT TCAAGTGGTC CTACATCCAT 
CAGCGATTGC TCAGCGATGT CCTCTTTTCG CTGGAAACGG ACATTCAGGT GTGGCGCAGT 
CATTCGACCA AAAGCGTCCT GGACTTTGTC AACTCCAGCG AGAATGCAAT TTTTGTGGTG 
AACACGGTGC ATTTAATATC GCAGCTAGCG GATAATCTGA TTATTGCCTG CGGAGGATTG 
CTGCCTCTTT TGGCCAGCGC CACGTCACCC AATTCCGAAT TGGATGTGCT GGAGCCAACG 
CAAGGCATGC CTTTGGAGGT GGCCGTATCT TTCCTGCAGC GTCTGGTCAA CATGGCGGAT 
GTCCTGATCT TTGCCACATC CCTAAACTTC GGCGAGTTGG AGGCGGAGAA GAACATGTCC 
AGTGGCGGCA TACTGCGTCA GTGCCTGCGA TTGGTTTGCA CCTGTGCGGT AAGGAATTGC 
TTGGAGTGCA AGGAGCGAAC GCGTTACAAT GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT 
GCAGCCCATT TGCAGGCCCT CATCCGCGGA GCTCAAGCAT CGCCCAAGAA CATTGTCGAG 
TCAATAACCG GCCAATTATC GCCTGTCAAG GATCCCGAGA AGCTGCTCCA GGACATGGAC 
GTCAATCGCC TGCGCGCAGT CATCTATCGC GATGTGGAGG AGACTAAGCA GGCGCAGTTC 
CTCTCGCTGG CGATAGTGTA CTTCATATCG GTGCTGATGG TGTCCAAGTA TCGTGATATC 
CTGGAGCCGC CGGCAGAGCC GCAAATCCAG CGTCAATCGC CAGTGCTGCA ACGCACGGCA 
GGCGGCGGTG GTCGTCAAAT CCAGGATAGT GACTACGAAA TAATTGTCGT CGATGAGAAC 
AATCCATCGG TTTTGGCGGA TAATGATTCA CATTCCAGTG GACCACCATC CATAAAGGCC 
AACCAGACAA CAACAATAGC AACAACAACT ACTACAACAG CAACAACCCA TATTAACAAC 
AACAACACTA AGACTACAAC ATCAAACGCT CCAACAGCAA CAATTAAAAT TCAGCAACAA 
CCATTGTCAC CACCAAAGCC GTTGGTGCCA CAAAAATTGC CAAAGAGCGT TGACTCGGAT 
GTGGGCTCCC TGAACATGAA CTCCACGGAA AACGAAGTGC CCGAGGTGGA GTCATCCAGC 
GAGATTCTAA TCGATGACCA CAAACCGAGT CACTCGAACG ACGAAAGCTG GACGGATGTG 
AATCTCAATG AGGATGCTGC CGTTCAGGCG GCAAGTGCCG GAATAGTTGT AGGTTTGGTG 
GATAACAGAG GTAATGTTAT ATCCGACAAA CACGATCCGT CCCATCACAA CCAACAACAG 
CAGCAGCAGG CGGGAATTAT TGGTCAGCAG CAGCAACATG GATCACTTGG TCATTCGGAG 
CGGGGTGACA AACCCGATTC GGAGATTTCG GTGGTGCGAG TGCCTGATGG TTATGGTGGT 
GCTGGTTCCG GTGGTGGCGT GAATTCCGGA CAAGGTCAGG GCGTTCCATC CAATCAGCGT 
CCTCGTCCCG AGGAGTTGCC CATGAAGGCG CCCGCCTTGG TGGCCCAACT GCCGCTGACC 
ACACCTTCGC GAGAGGCCAG TCTCACCCAG AAGCTGGAAA TCGCATTGGG GCCAGTGTGT 
CCGCTGCTCC GTGAAATCAT GGTGGACTTT GCGCCGTTCC TTTCGAAAAC CCTAGTCGGC 
TCCCATGGCC AGGAATTGCT GATGGAGGGC AAGGGTCTGA CGACCTTTAA GAACTCGCAT 
TCCGTCGTAG AGCTGGTCAT GTTGCTCTGC TCCCAGGAGT GGCAAAATAG CCTACAAAAG 
CACGCAGGAT TAGCCTTTAT TGAGCTAATC AATGAGGGTC GACTGCTTTC GCACGCCATG 
AAGGATCACA TTGTTCGGGT GGCCAATGAG GCGGAGTTCA TCCTCAATAG AATGCGAGCG 
GATGATGTCC TCAAGCATGC CGACTTTGAA TCGCAATGTG CACAGACACT TTTGGAGCGC 
AGGGAGGAGG AGCGAATGTG CGATCACTTG ATAACCGCCG CACGTCGCAG AGACAATGTG 
ATTGCCAGCC GACTGCTGGA GAAGGTACGA AACATCATGT GTAATCGCCA CGGAGCCTGG 
GGTGATTCCA GCTCCACATC CAGTGGTGGC GCCATCGTTG GAGCGGTGCA AAAGAGTCCC 
TACTGGAAGC TGGATGCCTG GGAGGATGAT GCCAGACGTC GGAAACGGAT GGTGCAAAAT 
CCGCGCGGAT CCTCGCATCC ACAGGCCACA CTGAAGGCGG CTTTGGAAAA TGGCGGACCT 
GAAGATGCCA TCCTGCAGAC TCGCGACGAG TTCCACACCC AAATAGCCGT CTCGCGGACT 
CATCCATCCG GTCAGCACAA TGGTGAGCTA CTGGACGACG CCGAGCTCTT GATTGAGGAT 
CGGGAGTTGG ATCTGGATCT CACGGGTCCC GTCAACATTA GCACCAAGGC GAGATTGATA 
GCCCCCGGTC TGGTGGCACC CGGCACCGTC TCAATAACCA GCACTGAAAT GTTCTTTGAG 
GTGGATGAAG AGCATCCCGA GTTCCAGAAG ATCGATGGCG AAGTTCTTAA GTACTGTGAC 
CATTTGCACG GCAAATGGTA CTTCTCCGAA GTGAGGGCCA TCTTCTCGAG GCGTTATCTC 
CTGCAAAATG TGGCCCTCGA GATCTTTTTG GCCAGCCGTA CGTCCATATT GTTTGCCTTT 
CCCGATCAGC ATACGGTCAA GAAGGTAATC AAGGCCCTGC CCCGTGTGGG AGTGGGCATC 
AAGTATGGAA TACCGCAGAC GAGGAGGGCC TCAATGATGT CGCCCCGTCA ACTGATGCGC 
AATTCGAACA TGACCCAAAA GTGGCAGCGT CGCGAGATTA GCAACTTTGA GTATCTTATG 
TTCCTGAACA CGATCGCCGG CAGGACGTAC AACGATCTAA ATCAGTATCC CATCTTTCCG 
TGGGTGCTGA CCAACTATGA GTCCAAGGAT TTGGATCTCA GCCTGCCGTC AAACTACAGG 
GATCTGTCGA AACCGATTGG CGCCCTGAAT CCATCGCGCA GAGCCTATTT CGAGGAGCGT 
TACGAGAGCT GGGACAGTGA CACCATACCG CCCTTCCACT ATGGTACGCA TTATTCCACG 
GCAGCATTTA CGCTGAACTG GTTGGTGCGC GTGGAACCGT TTACCACCAT GTTCCTGGCC 
CTGCAGGGCG GCAAATTTGA TTATCCGGAC AGGCTGTTCA GCTCGGTGTC GCTGTCCTGG 
AAGAATTGCC AACGTGATAC ATCGGATGTG AAGGAACTGA TACCAGAATG GTATTTCCTG 
CCCGAAATGT TTTACAATTC ATCAGGCTAT CGACTGGGTC ATCGCGAGGA TGGTGCCCTA 
GTGGATGACA TTGAACTACC ACCGTGGGCC AAGAGCCCCG AAGAATTCGT CCGCATCAAT 
CGCATGGCAC TGGAGTCGGA GTTCGTGTCC TGCCAGCTGC ACCAGTGGAT CGATTTGATC 
TTTGGCTACA AGCAACGCGG TCCCGAGGCC ATTAGGGCAA CCAACGTGTT CTACTATTTG 
ACATATGAGG GCAGCGTTGA CTTGGATGGC GTTTTGGATC CGGTGATGCG CGAAGCCGTC 
GAGAATCAAA TCCGCAACTT TGGCCAAACA CCTAGTCAAC TGCTAATGGA ACCGCATCCG 
CCGCGCAGCT CTGCGATGCA TCTATCGCCT ATGATGTTCA GTGCGATGCC GGAGGATCTC 
TGCCAGATGC TCAAGTTCTA TCAGAACTCA CCAGTCATTC ACATCTCGGC CAACACCTAT 
CCGCAACTGT CGTTGCCATC TGTGGTCACG GTAACGGCGG GTCATCAGTT TGCGGTGAAT 
CGATGGAACT GCAACTACAC CGCCTCCGTC CAGAGTCCCA GCTACGCCGA ATCGCCCCAA 
TCGCCGGGTT CCAATCAACC ACTGACCATC GATCCGGTTC TGGCTGTTCA TGGCACCAAC 
AACAATAGCA ATGCGGCCAG CCGACGTCAT TTGGGCGATA ACTTTAGCCA GATGCTAAAG 
ATACGCTCCA ACTGCTTTGT TACCACAGTG GACAGTCGGT TCCTAATCGC ATGCGGATTC 
TGGGACAACA GTTTCCGCGT CTTTGCCACC GAAACGGCGA AAATCGTGCA GATTGTATTC 
GGTCACTTTG GTGTGGTAAC GTGCATGGCA CGTTCCGAGT GCAACATCAC ATCAGACTGC 
TACATCGCCT CCGGATCGGC GGACTGCACG GTGCTTCTGT GGCACTGGAA TGCCCGAACC 
CAGAGCATTG TGGGCGAGGG CGATGTGCCC ACACCACGGG CCACCTTGAC GGGTCACGAA 
CAGGCCGTGA CATCGGTGGT GATTAGTGCC GAGTTGGGTC TGGTTGTGTC TGGATCATCA 
AATGGACCCG TACTTATCCA TACGACTTTT GGCGACCTGC TGCGCTCACT GGATCCACCA 
GCGGAGTTCC ATTCCCCGGA ACTGATAACC ATGTCCCGCG AGGGTTTCAT TGTCATCAAC 
TACGATAAGG GCAATGTGGC CGCCTACACC ATCAATGGCA AGAAACTGCG CCACGAGACG 
CACAACGACA ATCTACAGTG CATGCTACTG TCGCGCGATG GTGAATACCT GATGACCGCC 
GGTGATCGCG GCATCGTGGA GGTGTGGCGC ACCTTCAACC TAGCACCACT TTATGCCTTC 
CCCGCATGCA ACGCGGGCAT CCGATCTTTG GCTCTCACCC ACGATCAGAA ATACCTTCTG 
GCCGGACTTT CGACGGGCTC GATCATAGTA TTCCACATCG ACTTCAATCG CTGGCACCAC 
GAGTACCAGC AGCGCTACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rg ( NM_001144687.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138159.2 Drosophila melanogaster rugose (rg), transcript variant M, mRNA.

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CloneID	OFa22217
Clone ID Related Accession (Same CDS sequence)	NM_001144687.2
Accession Version	NM_001144687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform M
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144687.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGCTGTAC GATGGCAAAC TATCGAATGC CATTGTGTTC 
ATGTACAATC CGGTGGCCAC CGATGGTCAG TTGTGTCTGC AGTCATCGCC CAAGGGAAAC 
GTTTCATATT TCGTGCACAC GCCGCATGCG CTGATGTTGC AGGATGTGAA GGCCGTGGTC 
ACGCACTCGA TACACTGCAC CCTCAATTCG ATTGGCGGCA TCCAGGTGCT ATTCCCGCTT 
TTCTCGCAGC TGGATATGGC CCACGAGGGT CTGGGCGACA TTAAGCGGGA TCCCACGCTG 
TGCTCCAAGC TGCTGGGCTT CATCTGTGAA CTGGTGGAAA CATCGCAGAC GGTGCAGCAG 
CATATGATCC AGAACCGGGG ATTCCTGGTC ATCTCGTTCA TGCTGCAGCG CTCCTCCCGC 
GAACACCTGA CTCTCGAAGT CCTGGGATCA TTCCTGAATC TGACCAAGTA TCTGGTCACC 
TGCCTGTCGG CCAACAGTGA TCTGCTGCTC AAACAGTTGT TCTGTTTCTC GTTTCTCACG 
TGGCAGCTGC TGGATCACGT CCTCTTCAAT CCAGCCCTGT GGATATACAC ACCCGCCAAT 
GTCCAGGCGC GACTATACTC CTACTTGGCC ACCGAGTTCC TCTCGGACAC GCAGATCTAC 
AGCAATGTGA GGAGGGTCAG CACGGTACTG CAGACAGTGC ACACCCTAAA GTACTACTAC 
TGGGTGGTCA ATCCACGGGC TAAGAGCGGC ATCATTCCCA AGGGACTGGA TGGACCTCGT 
CCGGCTCAAA AGGACATCCT GGCCATCCGG GCATACATCC TGCTGTTCCT GAAGCAGCTC 
ATAATGATCG GCAATGGCGT GAAGGAGGAC GAGCTGCAGA GCATACTCAA CTATCTGACC 
ACCATGCACG AGGACGAGAA CCTGCACGAC GTGCTGCAGA TGCTCATCTC GCTGATGTCG 
GAGCATCCCA GCTCCATGGT ACCTGCCTTT GATGTGAAGC ACGGCGTGCG CAGCATCTTC 
AAGTTGCTGG CGGCCGAAAG TCAGTTGATT CGGCTTCAGG CCCTCAAGCT GCTGGGCTTC 
TTCCTTTCCC GCAGCACCCA CAAACGCAAG TACGATGTGA TGTCACCACA CAATCTGTAC 
ACCCTTTTAG CGGAGCGACT GCTTCTCTAT GAGGAATCAC TGTCCCTGCC CACATACAAT 
GTTCTCTATG AGATCATGAC GGAGCACATC TCGCAGCAGA TTCTATACAC ACGCCATCCG 
GAACCAGAGA GTCACTACCG TCTGGAGAAT CCAATGATCC TCAAGGTGGT GGCCACCCTG 
ATCCGGCAAT CAAAGCAGAC GGAATCGCTG ATCGACGTTA AAAAGCTATT CCTGCAGGAT 
ATGACCCTGT TGTGCAATAG CAATCGGGAG AACCGGCGCA CAGTGCTCCA GATGTCCGTG 
TGGCAGGAGT GGCTCATTGC GATGGCGTAC ATTCATCCAA AGAGCAGCGA GGAGCAGAAG 
ATAAGCGACA TGGTGTATTC CCTATTCCGC ATGCTGCTCC ACCATGCCAT TAAGCATGAA 
TACGGTGGCT GGAGAGTTTG GGTAGATACC CTGGCAATCG TCCACTCCAA GGTGTCTTAC 
GAGGAGTTTA AGCTCCAGTT TGCCCAGATG TACGAGCACT ACGAGCGCCA GCGAACGGAC 
AATATCACAG ATCCTGCCCT GCGTCAGGCT AGACCCATCA GCACGATCAG TGGTTGGGAG 
CGAGAGGAGC TGCATCAGCA GCAGAACGGT GGATCTGCAG CAGCAGTGGC TACCAATCAA 
ACGGCAGCTG TTAAAGGTTC TGTTTCCATT GCCTCACTGG AAGATGTGCC ACCGGTGGTT 
GAGGAAGAGG TGGAGGAACT GGAGCTGGAG GAAGTAGAGA TTCAAGAGGG TCCTATAACC 
GAAGAAACCG AACAAAAATC GGTGATAGCC AACATTTCCG ACGTCTACAA TGAGCAGCTC 
AAAACCGATG CCACGTGCAA TGGCAATTTG GAGGATGTCA AAGAGGAGGA GCCTGTACAA 
CAGCAAATCG GAGACCTGGA GAAGCAACCA GAGCCATCTA CTCCTTTGGG AGCACTCAGG 
GAAACCCTAC AACTTGGTGA TGATATGGAT GTGGAAGAAC TGGAGCTGGC TACCGCCAAG 
GATGCCCTCA ATGCGGAACA GCATGTGTCG CGAGTTCTTC AGGCTTCCGA AGCGGCACTT 
AACGATTGCA AAATGGCAGT CGATGATGTC CTGCAGGAGT CATCCTCCGT CCTCAAGGAC 
GAGGAGATCG AACTGGCCGT CAACGAGGTT GTTCAGGGTG TGCTCAATAA CGAGAAGAAA 
ACTCAATCCC AGGACAACAA GGATAACAAG GAACAGCCGG GCGAACAGGA TGTAAACGTC 
AGTCTCCTAA ACAGCAAGAA TCTGCTCAAC AATAATAATA ATAACAATAA CAACAGTCCG 
AGTCCAACGC CCACAACGGC GACTGCGACG GCGGAGACGG AAGCGGAAAC AGAAGTCAAT 
GCCAATGAGA TCGTGAGCAG CACAGAGGCG CCCAAGGCGG AAACGGAAAC GAGCGTAGCA 
CCGGAAGTAG AAACACCGGA AACGGCCAAG CCAAGCCCCA TTGTCCCCAG CCCCGTATTA 
GCAACCAATC AAAAGACTGA AGATGCAGCC AACAAGCTGA ACAACAACGA GAAGCTGGCC 
GAGATCAGCG CCAGTCCCGA GCCACCCATT GTCGTGGAAA CGCCAGAAGC TGATCTTCTT 
CAGCTTTCCG ATTCCGAAAC AAAACCGAAT AAGGAAACCG AAGCTGAAGA TTCGGTGGCG 
CTGGCCGTTA GAGATATTGT CGAGCAACTC ATCGACAAGG TCATCGATGC CACGGAAGCG 
GAATCGGCCA GCGAAACCAA AACGGAAACG AATAATAATG AAATACCCAA GAAGGAGAAG 
CAGACGTCAG AAGAGCCTGA GGATGTGGAA ACTGCAGAGA CTTTGGCCGC TGCCGCCAAG 
GAAATTGTCC AAGAGGTTGT GGAAGCAGCT CTCGTAATGG TCCAAGAGGA GAGCACTCAA 
GAGAAGCCAG AAAAGGGGGC GAACTCCGAA GAGGAGAAAA ACGAAATTGG CAAGGAAGAG 
ATTCTTCTGC AGCTGGAGGA AAAACCAGCG TCTACTGAAG TGCAGGAAAC CAAGATCGAA 
GGAGATCTAA AGAAACCAGA AGATCCAAAA GGTCACAGCT CTGTAGAACC AAAGACCCCG 
AACCTTGAGG AACCGAAACC CCAGGAAACT GAACAACAAA AGTCGCAGGA GGTCGCCGAA 
GAACTACCTC AAAAACCAGA GGAGCAGGTG GTGGCCATTG TCAACCAAGT CCTGGACACT 
CTGGTAGACG ATACGGTCAA GGCAGTGGCA GCCGAGCAAA CCACTCAAAC ATCGCCAGCC 
CCCGAAGAGC AATCGCCACA AATACTGGCA ATGGAATCAC CAGCAACGTC GGTGCGGGTC 
AAACCCACTG AGGTGGATTC CACCACCCAG ACTACGCCGA AGAACGAGGC TGGATCCAGT 
CTATTGGTTG AGCAGGTGCA GCAAGTCCTC CAGGAGGATG ACGCCCAGCA ATCAGCCGGC 
ATGACCATCG AAGATGAGGA TTACTCTAAT CAGCAGGCAG CTGCGGCAGT TGAAAATGCC 
AACAGCAGCC AATTGGATGC CAATCATTAT GGTCCCGGTA ATCCAGAATC CAAACAACAG 
CAACAGCGCT CCAAATCGGG ATCGACCCGA CCTATGTTCA GTCCGGGACC TACACGTCCG 
CCCTTCCGAA TACCGGAATT CAAGTGGTCC TACATCCATC AGCGATTGCT CAGCGATGTC 
CTCTTTTCGC TGGAAACGGA CATTCAGGTG TGGCGCAGTC ATTCGACCAA AAGCGTCCTG 
GACTTTGTCA ACTCCAGCGA GAATGCAATT TTTGTGGTGA ACACGGTGCA TTTAATATCG 
CAGCTAGCGG ATAATCTGAT TATTGCCTGC GGAGGATTGC TGCCTCTTTT GGCCAGCGCC 
ACGTCACCCA ATTCCGAATT GGATGTGCTG GAGCCAACGC AAGGCATGCC TTTGGAGGTG 
GCCGTATCTT TCCTGCAGCG TCTGGTCAAC ATGGCGGATG TCCTGATCTT TGCCACATCC 
CTAAACTTCG GCGAGTTGGA GGCGGAGAAG AACATGTCCA GTGGCGGCAT ACTGCGTCAG 
TGCCTGCGAT TGGTTTGCAC CTGTGCGGTA AGGAATTGCT TGGAGTGCAA GGAGCGAACG 
CGTTACAATG TGGGCGCCTT GGCGAGAGAT GTCCCGGGTG CAGCCCATTT GCAGGCCCTC 
ATCCGCGGAG CTCAAGCATC GCCCAAGAAC ATTGTCGAGT CAATAACCGG CCAATTATCG 
CCTGTCAAGG ATCCCGAGAA GCTGCTCCAG GACATGGACG TCAATCGCCT GCGCGCAGTC 
ATCTATCGCG ATGTGGAGGA GACTAAGCAG GCGCAGTTCC TCTCGCTGGC GATAGTGTAC 
TTCATATCGG TGCTGATGGT GTCCAAGTAT CGTGATATCC TGGAGCCGCC GGCAGAGCCG 
CAAATCCAGC GTCAATCGCC AGTGCTGCAA CGCACGGCAG GCGGCGGTGG TCGTCAAATC 
CAGGATAGTG ACTACGAAAT AATTGTCGTC GATGAGAACA ATCCATCGGT TTTGGCGGAT 
AATGATTCAC ATTCCAGTGG ACCACCATCC ATAAAGAGCG TTGACTCGGA TGTGGGCTCC 
CTGAACATGA ACTCCACGGA AAACGAAGTG CCCGAGGTGG AGTCATCCAG CGAGATTCTA 
ATCGATGACC ACAAACCGAG TCACTCGAAC GACGAAAGCT GGACGGATGT GAATCTCAAT 
GAGGATGCTG CCGTTCAGGC GGCAAGTGCC GGAATAGTTG TAGGTTTGGT GGATAACAGA 
GGTAATGTTA TATCCGACAA ACACGATCCG TCCCATCACA ACCAACAACA GCAGCAGCAG 
GCGGGAATTA TTGGTCAGCA GCAGCAACAT GGATCACTTG GTCATTCGGA GCGGGGTGAC 
AAACCCGATT CGGAGATTTC GGTGGTGCGA GTGCCTGATG GTTATGGTGG TGCTGGTTCC 
GGTGGTGGCG TGAATTCCGG ACAAGGTCAG GGCGTTCCAT CCAATCAGCG TCCTCGTCCC 
GAGGAGTTGC CCATGAAGGC GCCCGCCTTG GTGGCCCAAC TGCCGCTGAC CACACCTTCG 
CGAGAGGCCA GTCTCACCCA GAAGCTGGAA ATCGCATTGG GGCCAGTGTG TCCGCTGCTC 
CGTGAAATCA TGGTGGACTT TGCGCCGTTC CTTTCGAAAA CCCTAGTCGG CTCCCATGGC 
CAGGAATTGC TGATGGAGGG CAAGGGTCTG ACGACCTTTA AGAACTCGCA TTCCGTCGTA 
GAGCTGGTCA TGTTGCTCTG CTCCCAGGAG TGGCAAAATA GCCTACAAAA GCACGCAGGA 
TTAGCCTTTA TTGAGCTAAT CAATGAGGGT CGACTGCTTT CGCACGCCAT GAAGGATCAC 
ATTGTTCGGG TGGCCAATGA GGCGGAGTTC ATCCTCAATA GAATGCGAGC GGATGATGTC 
CTCAAGCATG CCGACTTTGA ATCGCAATGT GCACAGACAC TTTTGGAGCG CAGGGAGGAG 
GAGCGAATGT GCGATCACTT GATAACCGCC GCACGTCGCA GAGACAATGT GATTGCCAGC 
CGACTGCTGG AGAAGGTACG AAACATCATG TGTAATCGCC ACGGAGCCTG GGGTGATTCC 
AGCTCCACAT CCAGTGGTGG CGCCATCGTT GGAGCGGTGC AAAAGAGTCC CTACTGGAAG 
CTGGATGCCT GGGAGGATGA TGCCAGACGT CGGAAACGGA TGGTGCAAAA TCCGCGCGGA 
TCCTCGCATC CACAGGCCAC ACTGAAGGCG GCTTTGGAAA ATGGCGGACC TGAAGATGCC 
ATCCTGCAGA CTCGCGACGA GTTCCACACC CAAATAGCCG TCTCGCGGAC TCATCCATCC 
GGTCAGCACA ATGGTGAGCT ACTGGACGAC GCCGAGCTCT TGATTGAGGA TCGGGAGTTG 
GATCTGGATC TCACGGGTCC CGTCAACATT AGCACCAAGG CGAGATTGAT AGCCCCCGGT 
CTGGTGGCAC CCGGCACCGT CTCAATAACC AGCACTGAAA TGTTCTTTGA GGTGGATGAA 
GAGCATCCCG AGTTCCAGAA GATCGATGGC GAAGTTCTTA AGTACTGTGA CCATTTGCAC 
GGCAAATGGT ACTTCTCCGA AGTGAGGGCC ATCTTCTCGA GGCGTTATCT CCTGCAAAAT 
GTGGCCCTCG AGATCTTTTT GGCCAGCCGT ACGTCCATAT TGTTTGCCTT TCCCGATCAG 
CATACGGTCA AGAAGGTAAT CAAGGCCCTG CCCCGTGTGG GAGTGGGCAT CAAGTATGGA 
ATACCGCAGA CGAGGAGGGC CTCAATGATG TCGCCCCGTC AACTGATGCG CAATTCGAAC 
ATGACCCAAA AGTGGCAGCG TCGCGAGATT AGCAACTTTG AGTATCTTAT GTTCCTGAAC 
ACGATCGCCG GCAGGACGTA CAACGATCTA AATCAGTATC CCATCTTTCC GTGGGTGCTG 
ACCAACTATG AGTCCAAGGA TTTGGATCTC AGCCTGCCGT CAAACTACAG GGATCTGTCG 
AAACCGATTG GCGCCCTGAA TCCATCGCGC AGAGCCTATT TCGAGGAGCG TTACGAGAGC 
TGGGACAGTG ACACCATACC GCCCTTCCAC TATGGTACGC ATTATTCCAC GGCAGCATTT 
ACGCTGAACT GGTTGGTGCG CGTGGAACCG TTTACCACCA TGTTCCTGGC CCTGCAGGGC 
GGCAAATTTG ATTATCCGGA CAGGCTGTTC AGCTCGGTGT CGCTGTCCTG GAAGAATTGC 
CAACGTGATA CATCGGATGT GAAGGAACTG ATACCAGAAT GGTATTTCCT GCCCGAAATG 
TTTTACAATT CATCAGGCTA TCGACTGGGT CATCGCGAGG ATGGTGCCCT AGTGGATGAC 
ATTGAACTAC CACCGTGGGC CAAGAGCCCC GAAGAATTCG TCCGCATCAA TCGCATGGCA 
CTGGAGTCGG AGTTCGTGTC CTGCCAGCTG CACCAGTGGA TCGATTTGAT CTTTGGCTAC 
AAGCAACGCG GTCCCGAGGC CATTAGGGCA ACCAACGTGT TCTACTATTT GACATATGAG 
GGCAGCGTTG ACTTGGATGG CGTTTTGGAT CCGGTGATGC GCGAAGCCGT CGAGAATCAA 
ATCCGCAACT TTGGCCAAAC ACCTAGTCAA CTGCTAATGG AACCGCATCC GCCGCGCAGC 
TCTGCGATGC ATCTATCGCC TATGATGTTC AGTGCGATGC CGGAGGATCT CTGCCAGATG 
CTCAAGTTCT ATCAGAACTC ACCAGTCATT CACATCTCGG CCAACACCTA TCCGCAACTG 
TCGTTGCCAT CTGTGGTCAC GGTAACGGCG GGTCATCAGT TTGCGGTGAA TCGATGGAAC 
TGCAACTACA CCGCCTCCGT CCAGAGTCCC AGCTACGCCG AATCGCCCCA ATCGCCGGGT 
TCCAATCAAC CACTGACCAT CGATCCGGTT CTGGCTGTTC ATGGCACCAA CAACAATAGC 
AATGCGGCCA GCCGACGTCA TTTGGGCGAT AACTTTAGCC AGATGCTAAA GATACGCTCC 
AACTGCTTTG TTACCACAGT GGACAGTCGG TTCCTAATCG CATGCGGATT CTGGGACAAC 
AGTTTCCGCG TCTTTGCCAC CGAAACGGCG AAAATCGTGC AGATTGTATT CGGTCACTTT 
GGTGTGGTAA CGTGCATGGC ACGTTCCGAG TGCAACATCA CATCAGACTG CTACATCGCC 
TCCGGATCGG CGGACTGCAC GGTGCTTCTG TGGCACTGGA ATGCCCGAAC CCAGAGCATT 
GTGGGCGAGG GCGATGTGCC CACACCACGG GCCACCTTGA CGGGTCACGA ACAGGCCGTG 
ACATCGGTGG TGATTAGTGC CGAGTTGGGT CTGGTTGTGT CTGGATCATC AAATGGACCC 
GTACTTATCC ATACGACTTT TGGCGACCTG CTGCGCTCAC TGGATCCACC AGCGGAGTTC 
CATTCCCCGG AACTGATAAC CATGTCCCGC GAGGGTTTCA TTGTCATCAA CTACGATAAG 
GGCAATGTGG CCGCCTACAC CATCAATGGC AAGAAACTGC GCCACGAGAC GCACAACGAC 
AATCTACAGT GCATGCTACT GTCGCGCGAT GGTGAATACC TGATGACCGC CGGTGATCGC 
GGCATCGTGG AGGTGTGGCG CACCTTCAAC CTAGCACCAC TTTATGCCTT CCCCGCATGC 
AACGCGGGCA TCCGATCTTT GGCTCTCACC CACGATCAGA AATACCTTCT GGCCGGACTT 
TCGACGGGCT CGATCATAGT ATTCCACATC GACTTCAATC GCTGGCACCA CGAGTACCAG 
CAGCGCTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138159.2
CDS	1089..10700
Translation	MADIMRPPYSEIKRPDEIVRMTTADNLKFAVLIGLIEVGQVTNREVVNTVLHLLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLFCFSFLTWQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_001144687.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant M, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144687.2

ORF Insert Sequence:

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGCTGTAC GATGGCAAAC TATCGAATGC CATTGTGTTC 
ATGTACAATC CGGTGGCCAC CGATGGTCAG TTGTGTCTGC AGTCATCGCC CAAGGGAAAC 
GTTTCATATT TCGTGCACAC GCCGCATGCG CTGATGTTGC AGGATGTGAA GGCCGTGGTC 
ACGCACTCGA TACACTGCAC CCTCAATTCG ATTGGCGGCA TCCAGGTGCT ATTCCCGCTT 
TTCTCGCAGC TGGATATGGC CCACGAGGGT CTGGGCGACA TTAAGCGGGA TCCCACGCTG 
TGCTCCAAGC TGCTGGGCTT CATCTGTGAA CTGGTGGAAA CATCGCAGAC GGTGCAGCAG 
CATATGATCC AGAACCGGGG ATTCCTGGTC ATCTCGTTCA TGCTGCAGCG CTCCTCCCGC 
GAACACCTGA CTCTCGAAGT CCTGGGATCA TTCCTGAATC TGACCAAGTA TCTGGTCACC 
TGCCTGTCGG CCAACAGTGA TCTGCTGCTC AAACAGTTGT TCTGTTTCTC GTTTCTCACG 
TGGCAGCTGC TGGATCACGT CCTCTTCAAT CCAGCCCTGT GGATATACAC ACCCGCCAAT 
GTCCAGGCGC GACTATACTC CTACTTGGCC ACCGAGTTCC TCTCGGACAC GCAGATCTAC 
AGCAATGTGA GGAGGGTCAG CACGGTACTG CAGACAGTGC ACACCCTAAA GTACTACTAC 
TGGGTGGTCA ATCCACGGGC TAAGAGCGGC ATCATTCCCA AGGGACTGGA TGGACCTCGT 
CCGGCTCAAA AGGACATCCT GGCCATCCGG GCATACATCC TGCTGTTCCT GAAGCAGCTC 
ATAATGATCG GCAATGGCGT GAAGGAGGAC GAGCTGCAGA GCATACTCAA CTATCTGACC 
ACCATGCACG AGGACGAGAA CCTGCACGAC GTGCTGCAGA TGCTCATCTC GCTGATGTCG 
GAGCATCCCA GCTCCATGGT ACCTGCCTTT GATGTGAAGC ACGGCGTGCG CAGCATCTTC 
AAGTTGCTGG CGGCCGAAAG TCAGTTGATT CGGCTTCAGG CCCTCAAGCT GCTGGGCTTC 
TTCCTTTCCC GCAGCACCCA CAAACGCAAG TACGATGTGA TGTCACCACA CAATCTGTAC 
ACCCTTTTAG CGGAGCGACT GCTTCTCTAT GAGGAATCAC TGTCCCTGCC CACATACAAT 
GTTCTCTATG AGATCATGAC GGAGCACATC TCGCAGCAGA TTCTATACAC ACGCCATCCG 
GAACCAGAGA GTCACTACCG TCTGGAGAAT CCAATGATCC TCAAGGTGGT GGCCACCCTG 
ATCCGGCAAT CAAAGCAGAC GGAATCGCTG ATCGACGTTA AAAAGCTATT CCTGCAGGAT 
ATGACCCTGT TGTGCAATAG CAATCGGGAG AACCGGCGCA CAGTGCTCCA GATGTCCGTG 
TGGCAGGAGT GGCTCATTGC GATGGCGTAC ATTCATCCAA AGAGCAGCGA GGAGCAGAAG 
ATAAGCGACA TGGTGTATTC CCTATTCCGC ATGCTGCTCC ACCATGCCAT TAAGCATGAA 
TACGGTGGCT GGAGAGTTTG GGTAGATACC CTGGCAATCG TCCACTCCAA GGTGTCTTAC 
GAGGAGTTTA AGCTCCAGTT TGCCCAGATG TACGAGCACT ACGAGCGCCA GCGAACGGAC 
AATATCACAG ATCCTGCCCT GCGTCAGGCT AGACCCATCA GCACGATCAG TGGTTGGGAG 
CGAGAGGAGC TGCATCAGCA GCAGAACGGT GGATCTGCAG CAGCAGTGGC TACCAATCAA 
ACGGCAGCTG TTAAAGGTTC TGTTTCCATT GCCTCACTGG AAGATGTGCC ACCGGTGGTT 
GAGGAAGAGG TGGAGGAACT GGAGCTGGAG GAAGTAGAGA TTCAAGAGGG TCCTATAACC 
GAAGAAACCG AACAAAAATC GGTGATAGCC AACATTTCCG ACGTCTACAA TGAGCAGCTC 
AAAACCGATG CCACGTGCAA TGGCAATTTG GAGGATGTCA AAGAGGAGGA GCCTGTACAA 
CAGCAAATCG GAGACCTGGA GAAGCAACCA GAGCCATCTA CTCCTTTGGG AGCACTCAGG 
GAAACCCTAC AACTTGGTGA TGATATGGAT GTGGAAGAAC TGGAGCTGGC TACCGCCAAG 
GATGCCCTCA ATGCGGAACA GCATGTGTCG CGAGTTCTTC AGGCTTCCGA AGCGGCACTT 
AACGATTGCA AAATGGCAGT CGATGATGTC CTGCAGGAGT CATCCTCCGT CCTCAAGGAC 
GAGGAGATCG AACTGGCCGT CAACGAGGTT GTTCAGGGTG TGCTCAATAA CGAGAAGAAA 
ACTCAATCCC AGGACAACAA GGATAACAAG GAACAGCCGG GCGAACAGGA TGTAAACGTC 
AGTCTCCTAA ACAGCAAGAA TCTGCTCAAC AATAATAATA ATAACAATAA CAACAGTCCG 
AGTCCAACGC CCACAACGGC GACTGCGACG GCGGAGACGG AAGCGGAAAC AGAAGTCAAT 
GCCAATGAGA TCGTGAGCAG CACAGAGGCG CCCAAGGCGG AAACGGAAAC GAGCGTAGCA 
CCGGAAGTAG AAACACCGGA AACGGCCAAG CCAAGCCCCA TTGTCCCCAG CCCCGTATTA 
GCAACCAATC AAAAGACTGA AGATGCAGCC AACAAGCTGA ACAACAACGA GAAGCTGGCC 
GAGATCAGCG CCAGTCCCGA GCCACCCATT GTCGTGGAAA CGCCAGAAGC TGATCTTCTT 
CAGCTTTCCG ATTCCGAAAC AAAACCGAAT AAGGAAACCG AAGCTGAAGA TTCGGTGGCG 
CTGGCCGTTA GAGATATTGT CGAGCAACTC ATCGACAAGG TCATCGATGC CACGGAAGCG 
GAATCGGCCA GCGAAACCAA AACGGAAACG AATAATAATG AAATACCCAA GAAGGAGAAG 
CAGACGTCAG AAGAGCCTGA GGATGTGGAA ACTGCAGAGA CTTTGGCCGC TGCCGCCAAG 
GAAATTGTCC AAGAGGTTGT GGAAGCAGCT CTCGTAATGG TCCAAGAGGA GAGCACTCAA 
GAGAAGCCAG AAAAGGGGGC GAACTCCGAA GAGGAGAAAA ACGAAATTGG CAAGGAAGAG 
ATTCTTCTGC AGCTGGAGGA AAAACCAGCG TCTACTGAAG TGCAGGAAAC CAAGATCGAA 
GGAGATCTAA AGAAACCAGA AGATCCAAAA GGTCACAGCT CTGTAGAACC AAAGACCCCG 
AACCTTGAGG AACCGAAACC CCAGGAAACT GAACAACAAA AGTCGCAGGA GGTCGCCGAA 
GAACTACCTC AAAAACCAGA GGAGCAGGTG GTGGCCATTG TCAACCAAGT CCTGGACACT 
CTGGTAGACG ATACGGTCAA GGCAGTGGCA GCCGAGCAAA CCACTCAAAC ATCGCCAGCC 
CCCGAAGAGC AATCGCCACA AATACTGGCA ATGGAATCAC CAGCAACGTC GGTGCGGGTC 
AAACCCACTG AGGTGGATTC CACCACCCAG ACTACGCCGA AGAACGAGGC TGGATCCAGT 
CTATTGGTTG AGCAGGTGCA GCAAGTCCTC CAGGAGGATG ACGCCCAGCA ATCAGCCGGC 
ATGACCATCG AAGATGAGGA TTACTCTAAT CAGCAGGCAG CTGCGGCAGT TGAAAATGCC 
AACAGCAGCC AATTGGATGC CAATCATTAT GGTCCCGGTA ATCCAGAATC CAAACAACAG 
CAACAGCGCT CCAAATCGGG ATCGACCCGA CCTATGTTCA GTCCGGGACC TACACGTCCG 
CCCTTCCGAA TACCGGAATT CAAGTGGTCC TACATCCATC AGCGATTGCT CAGCGATGTC 
CTCTTTTCGC TGGAAACGGA CATTCAGGTG TGGCGCAGTC ATTCGACCAA AAGCGTCCTG 
GACTTTGTCA ACTCCAGCGA GAATGCAATT TTTGTGGTGA ACACGGTGCA TTTAATATCG 
CAGCTAGCGG ATAATCTGAT TATTGCCTGC GGAGGATTGC TGCCTCTTTT GGCCAGCGCC 
ACGTCACCCA ATTCCGAATT GGATGTGCTG GAGCCAACGC AAGGCATGCC TTTGGAGGTG 
GCCGTATCTT TCCTGCAGCG TCTGGTCAAC ATGGCGGATG TCCTGATCTT TGCCACATCC 
CTAAACTTCG GCGAGTTGGA GGCGGAGAAG AACATGTCCA GTGGCGGCAT ACTGCGTCAG 
TGCCTGCGAT TGGTTTGCAC CTGTGCGGTA AGGAATTGCT TGGAGTGCAA GGAGCGAACG 
CGTTACAATG TGGGCGCCTT GGCGAGAGAT GTCCCGGGTG CAGCCCATTT GCAGGCCCTC 
ATCCGCGGAG CTCAAGCATC GCCCAAGAAC ATTGTCGAGT CAATAACCGG CCAATTATCG 
CCTGTCAAGG ATCCCGAGAA GCTGCTCCAG GACATGGACG TCAATCGCCT GCGCGCAGTC 
ATCTATCGCG ATGTGGAGGA GACTAAGCAG GCGCAGTTCC TCTCGCTGGC GATAGTGTAC 
TTCATATCGG TGCTGATGGT GTCCAAGTAT CGTGATATCC TGGAGCCGCC GGCAGAGCCG 
CAAATCCAGC GTCAATCGCC AGTGCTGCAA CGCACGGCAG GCGGCGGTGG TCGTCAAATC 
CAGGATAGTG ACTACGAAAT AATTGTCGTC GATGAGAACA ATCCATCGGT TTTGGCGGAT 
AATGATTCAC ATTCCAGTGG ACCACCATCC ATAAAGAGCG TTGACTCGGA TGTGGGCTCC 
CTGAACATGA ACTCCACGGA AAACGAAGTG CCCGAGGTGG AGTCATCCAG CGAGATTCTA 
ATCGATGACC ACAAACCGAG TCACTCGAAC GACGAAAGCT GGACGGATGT GAATCTCAAT 
GAGGATGCTG CCGTTCAGGC GGCAAGTGCC GGAATAGTTG TAGGTTTGGT GGATAACAGA 
GGTAATGTTA TATCCGACAA ACACGATCCG TCCCATCACA ACCAACAACA GCAGCAGCAG 
GCGGGAATTA TTGGTCAGCA GCAGCAACAT GGATCACTTG GTCATTCGGA GCGGGGTGAC 
AAACCCGATT CGGAGATTTC GGTGGTGCGA GTGCCTGATG GTTATGGTGG TGCTGGTTCC 
GGTGGTGGCG TGAATTCCGG ACAAGGTCAG GGCGTTCCAT CCAATCAGCG TCCTCGTCCC 
GAGGAGTTGC CCATGAAGGC GCCCGCCTTG GTGGCCCAAC TGCCGCTGAC CACACCTTCG 
CGAGAGGCCA GTCTCACCCA GAAGCTGGAA ATCGCATTGG GGCCAGTGTG TCCGCTGCTC 
CGTGAAATCA TGGTGGACTT TGCGCCGTTC CTTTCGAAAA CCCTAGTCGG CTCCCATGGC 
CAGGAATTGC TGATGGAGGG CAAGGGTCTG ACGACCTTTA AGAACTCGCA TTCCGTCGTA 
GAGCTGGTCA TGTTGCTCTG CTCCCAGGAG TGGCAAAATA GCCTACAAAA GCACGCAGGA 
TTAGCCTTTA TTGAGCTAAT CAATGAGGGT CGACTGCTTT CGCACGCCAT GAAGGATCAC 
ATTGTTCGGG TGGCCAATGA GGCGGAGTTC ATCCTCAATA GAATGCGAGC GGATGATGTC 
CTCAAGCATG CCGACTTTGA ATCGCAATGT GCACAGACAC TTTTGGAGCG CAGGGAGGAG 
GAGCGAATGT GCGATCACTT GATAACCGCC GCACGTCGCA GAGACAATGT GATTGCCAGC 
CGACTGCTGG AGAAGGTACG AAACATCATG TGTAATCGCC ACGGAGCCTG GGGTGATTCC 
AGCTCCACAT CCAGTGGTGG CGCCATCGTT GGAGCGGTGC AAAAGAGTCC CTACTGGAAG 
CTGGATGCCT GGGAGGATGA TGCCAGACGT CGGAAACGGA TGGTGCAAAA TCCGCGCGGA 
TCCTCGCATC CACAGGCCAC ACTGAAGGCG GCTTTGGAAA ATGGCGGACC TGAAGATGCC 
ATCCTGCAGA CTCGCGACGA GTTCCACACC CAAATAGCCG TCTCGCGGAC TCATCCATCC 
GGTCAGCACA ATGGTGAGCT ACTGGACGAC GCCGAGCTCT TGATTGAGGA TCGGGAGTTG 
GATCTGGATC TCACGGGTCC CGTCAACATT AGCACCAAGG CGAGATTGAT AGCCCCCGGT 
CTGGTGGCAC CCGGCACCGT CTCAATAACC AGCACTGAAA TGTTCTTTGA GGTGGATGAA 
GAGCATCCCG AGTTCCAGAA GATCGATGGC GAAGTTCTTA AGTACTGTGA CCATTTGCAC 
GGCAAATGGT ACTTCTCCGA AGTGAGGGCC ATCTTCTCGA GGCGTTATCT CCTGCAAAAT 
GTGGCCCTCG AGATCTTTTT GGCCAGCCGT ACGTCCATAT TGTTTGCCTT TCCCGATCAG 
CATACGGTCA AGAAGGTAAT CAAGGCCCTG CCCCGTGTGG GAGTGGGCAT CAAGTATGGA 
ATACCGCAGA CGAGGAGGGC CTCAATGATG TCGCCCCGTC AACTGATGCG CAATTCGAAC 
ATGACCCAAA AGTGGCAGCG TCGCGAGATT AGCAACTTTG AGTATCTTAT GTTCCTGAAC 
ACGATCGCCG GCAGGACGTA CAACGATCTA AATCAGTATC CCATCTTTCC GTGGGTGCTG 
ACCAACTATG AGTCCAAGGA TTTGGATCTC AGCCTGCCGT CAAACTACAG GGATCTGTCG 
AAACCGATTG GCGCCCTGAA TCCATCGCGC AGAGCCTATT TCGAGGAGCG TTACGAGAGC 
TGGGACAGTG ACACCATACC GCCCTTCCAC TATGGTACGC ATTATTCCAC GGCAGCATTT 
ACGCTGAACT GGTTGGTGCG CGTGGAACCG TTTACCACCA TGTTCCTGGC CCTGCAGGGC 
GGCAAATTTG ATTATCCGGA CAGGCTGTTC AGCTCGGTGT CGCTGTCCTG GAAGAATTGC 
CAACGTGATA CATCGGATGT GAAGGAACTG ATACCAGAAT GGTATTTCCT GCCCGAAATG 
TTTTACAATT CATCAGGCTA TCGACTGGGT CATCGCGAGG ATGGTGCCCT AGTGGATGAC 
ATTGAACTAC CACCGTGGGC CAAGAGCCCC GAAGAATTCG TCCGCATCAA TCGCATGGCA 
CTGGAGTCGG AGTTCGTGTC CTGCCAGCTG CACCAGTGGA TCGATTTGAT CTTTGGCTAC 
AAGCAACGCG GTCCCGAGGC CATTAGGGCA ACCAACGTGT TCTACTATTT GACATATGAG 
GGCAGCGTTG ACTTGGATGG CGTTTTGGAT CCGGTGATGC GCGAAGCCGT CGAGAATCAA 
ATCCGCAACT TTGGCCAAAC ACCTAGTCAA CTGCTAATGG AACCGCATCC GCCGCGCAGC 
TCTGCGATGC ATCTATCGCC TATGATGTTC AGTGCGATGC CGGAGGATCT CTGCCAGATG 
CTCAAGTTCT ATCAGAACTC ACCAGTCATT CACATCTCGG CCAACACCTA TCCGCAACTG 
TCGTTGCCAT CTGTGGTCAC GGTAACGGCG GGTCATCAGT TTGCGGTGAA TCGATGGAAC 
TGCAACTACA CCGCCTCCGT CCAGAGTCCC AGCTACGCCG AATCGCCCCA ATCGCCGGGT 
TCCAATCAAC CACTGACCAT CGATCCGGTT CTGGCTGTTC ATGGCACCAA CAACAATAGC 
AATGCGGCCA GCCGACGTCA TTTGGGCGAT AACTTTAGCC AGATGCTAAA GATACGCTCC 
AACTGCTTTG TTACCACAGT GGACAGTCGG TTCCTAATCG CATGCGGATT CTGGGACAAC 
AGTTTCCGCG TCTTTGCCAC CGAAACGGCG AAAATCGTGC AGATTGTATT CGGTCACTTT 
GGTGTGGTAA CGTGCATGGC ACGTTCCGAG TGCAACATCA CATCAGACTG CTACATCGCC 
TCCGGATCGG CGGACTGCAC GGTGCTTCTG TGGCACTGGA ATGCCCGAAC CCAGAGCATT 
GTGGGCGAGG GCGATGTGCC CACACCACGG GCCACCTTGA CGGGTCACGA ACAGGCCGTG 
ACATCGGTGG TGATTAGTGC CGAGTTGGGT CTGGTTGTGT CTGGATCATC AAATGGACCC 
GTACTTATCC ATACGACTTT TGGCGACCTG CTGCGCTCAC TGGATCCACC AGCGGAGTTC 
CATTCCCCGG AACTGATAAC CATGTCCCGC GAGGGTTTCA TTGTCATCAA CTACGATAAG 
GGCAATGTGG CCGCCTACAC CATCAATGGC AAGAAACTGC GCCACGAGAC GCACAACGAC 
AATCTACAGT GCATGCTACT GTCGCGCGAT GGTGAATACC TGATGACCGC CGGTGATCGC 
GGCATCGTGG AGGTGTGGCG CACCTTCAAC CTAGCACCAC TTTATGCCTT CCCCGCATGC 
AACGCGGGCA TCCGATCTTT GGCTCTCACC CACGATCAGA AATACCTTCT GGCCGGACTT 
TCGACGGGCT CGATCATAGT ATTCCACATC GACTTCAATC GCTGGCACCA CGAGTACCAG 
CAGCGCTACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rg ( NM_001042796.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036261.3 Drosophila melanogaster rugose (rg), transcript variant L, mRNA.

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CloneID	OFa22218
Clone ID Related Accession (Same CDS sequence)	NM_001042796.3
Accession Version	NM_001042796.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform L
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001042796.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGCTG 
CTGGATCACG TCCTCTTCAA TCCAGCCCTG TGGATATACA CACCCGCCAA TGTCCAGGCG 
CGACTATACT CCTACTTGGC CACCGAGTTC CTCTCGGACA CGCAGATCTA CAGCAATGTG 
AGGAGGGTCA GCACGGTACT GCAGACAGTG CACACCCTAA AGTACTACTA CTGGGTGGTC 
AATCCACGGG CTAAGAGCGG CATCATTCCC AAGGGACTGG ATGGACCTCG TCCGGCTCAA 
AAGGACATCC TGGCCATCCG GGCATACATC CTGCTGTTCC TGAAGCAGCT CATAATGATC 
GGCAATGGCG TGAAGGAGGA CGAGCTGCAG AGCATACTCA ACTATCTGAC CACCATGCAC 
GAGGACGAGA ACCTGCACGA CGTGCTGCAG ATGCTCATCT CGCTGATGTC GGAGCATCCC 
AGCTCCATGG TACCTGCCTT TGATGTGAAG CACGGCGTGC GCAGCATCTT CAAGTTGCTG 
GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC 
CGCAGCACCC ACAAACGCAA GTACGATGTG ATGTCACCAC ACAATCTGTA CACCCTTTTA 
GCGGAGCGAC TGCTTCTCTA TGAGGAATCA CTGTCCCTGC CCACATACAA TGTTCTCTAT 
GAGATCATGA CGGAGCACAT CTCGCAGCAG ATTCTATACA CACGCCATCC GGAACCAGAG 
AGTCACTACC GTCTGGAGAA TCCAATGATC CTCAAGGTGG TGGCCACCCT GATCCGGCAA 
TCAAAGCAGA CGGAATCGCT GATCGACGTT AAAAAGCTAT TCCTGCAGGA TATGACCCTG 
TTGTGCAATA GCAATCGGGA GAACCGGCGC ACAGTGCTCC AGATGTCCGT GTGGCAGGAG 
TGGCTCATTG CGATGGCGTA CATTCATCCA AAGAGCAGCG AGGAGCAGAA GATAAGCGAC 
ATGGTGTATT CCCTATTCCG CATGCTGCTC CACCATGCCA TTAAGCATGA ATACGGTGGC 
TGGAGAGTTT GGGTAGATAC CCTGGCAATC GTCCACTCCA AGGTGTCTTA CGAGGAGTTT 
AAGCTCCAGT TTGCCCAGAT GTACGAGCAC TACGAGCGCC AGCGAACGGA CAATATCACA 
GATCCTGCCC TGCGTCAGGC TAGACCCATC AGCACGATCA GTGGTTGGGA GCGAGAGGAG 
CTGCATCAGC AGCAGAACGG TGGATCTGCA GCAGCAGTGG CTACCAATCA AACGGCAGCT 
GTTAAAGGTT CTGTTTCCAT TGCCTCACTG GAAGATGTGC CACCGGTGGT TGAGGAAGAG 
GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG ATTCAAGAGG GTCCTATAAC CGAAGAAACC 
GAACAAAAAT CGGTGATAGC CAACATTTCC GACGTCTACA ATGAGCAGCT CAAAACCGAT 
GCCACGTGCA ATGGCAATTT GGAGGATGTC AAAGAGGAGG AGCCTGTACA ACAGCAAATC 
GGAGACCTGG AGAAGCAACC AGAGCCATCT ACTCCTTTGG GAGCACTCAG GGAAACCCTA 
CAACTTGGTG ATGATATGGA TGTGGAAGAA CTGGAGCTGG CTACCGCCAA GGATGCCCTC 
AATGCGGAAC AGCATGTGTC GCGAGTTCTT CAGGCTTCCG AAGCGGCACT TAACGATTGC 
AAAATGGCAG TCGATGATGT CCTGCAGGAG TCATCCTCCG TCCTCAAGGA CGAGGAGATC 
GAACTGGCCG TCAACGAGGT TGTTCAGGGT GTGCTCAATA ACGAGAAGAA AACTCAATCC 
CAGGACAACA AGGATAACAA GGAACAGCCG GGCGAACAGG ATGTAAACGT CAGTCTCCTA 
AACAGCAAGA ATCTGCTCAA CAATAATAAT AATAACAATA ACAACAGTCC GAGTCCAACG 
CCCACAACGG CGACTGCGAC GGCGGAGACG GAAGCGGAAA CAGAAGTCAA TGCCAATGAG 
ATCGTGAGCA GCACAGAGGC GCCCAAGGCG GAAACGGAAA CGAGCGTAGC ACCGGAAGTA 
GAAACACCGG AAACGGCCAA GCCAAGCCCC ATTGTCCCCA GCCCCGTATT AGCAACCAAT 
CAAAAGACTG AAGATGCAGC CAACAAGCTG AACAACAACG AGAAGCTGGC CGAGATCAGC 
GCCAGTCCCG AGCCACCCAT TGTCGTGGAA ACGCCAGAAG CTGATCTTCT TCAGCTTTCC 
GATTCCGAAA CAAAACCGAA TAAGGAAACC GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT 
AGAGATATTG TCGAGCAACT CATCGACAAG GTCATCGATG CCACGGAAGC GGAATCGGCC 
AGCGAAACCA AAACGGAAAC GAATAATAAT GAAATACCCA AGAAGGAGAA GCAGACGTCA 
GAAGAGCCTG AGGATGTGGA AACTGCAGAG ACTTTGGCCG CTGCCGCCAA GGAAATTGTC 
CAAGAGGTTG TGGAAGCAGC TCTCGTAATG GTCCAAGAGG AGAGCACTCA AGAGAAGCCA 
GAAAAGGGGG CGAACTCCGA AGAGGAGAAA AACGAAATTG GCAAGGAAGA GATTCTTCTG 
CAGCTGGAGG AAAAACCAGC GTCTACTGAA GTGCAGGAAA CCAAGATCGA AGGAGATCTA 
AAGAAACCAG AAGATCCAAA AGGTCACAGC TCTGTAGAAC CAAAGACCCC GAACCTTGAG 
GAACCGAAAC CCCAGGAAAC TGAACAACAA AAGTCGCAGG AGGTCGCCGA AGAACTACCT 
CAAAAACCAG AGGAGCAGGT GGTGGCCATT GTCAACCAAG TCCTGGACAC TCTGGTAGAC 
GATACGGTCA AGGCAGTGGC AGCCGAGCAA ACCACTCAAA CATCGCCAGC CCCCGAAGAG 
CAATCGCCAC AAATACTGGC AATGGAATCA CCAGCAACGT CGGTGCGGGT CAAACCCACT 
GAGGTGGATT CCACCACCCA GACTACGCCG AAGAACGAGG CTGGATCCAG TCTATTGGTT 
GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT GACGCCCAGC AATCAGCCGG CATGACCATC 
GAAGATGAGG ATTACTCTAA TCAGCAGGCA GCTGCGGCAG TTGAAAATGC CAACAGCAGC 
CAATTGGATG CCAATCATTA TGGTCCCGGT AATCCAGAAT CCAAACAACA GCAACAGCGC 
TCCAAATCGG GATCGACCCG ACCTATGTTC AGTCCGGGAC CTACACGTCC GCCCTTCCGA 
ATACCGGAAT TCAAGTGGTC CTACATCCAT CAGCGATTGC TCAGCGATGT CCTCTTTTCG 
CTGGAAACGG ACATTCAGGT GTGGCGCAGT CATTCGACCA AAAGCGTCCT GGACTTTGTC 
AACTCCAGCG AGAATGCAAT TTTTGTGGTG AACACGGTGC ATTTAATATC GCAGCTAGCG 
GATAATCTGA TTATTGCCTG CGGAGGATTG CTGCCTCTTT TGGCCAGCGC CACGTCACCC 
AATTCCGAAT TGGATGTGCT GGAGCCAACG CAAGGCATGC CTTTGGAGGT GGCCGTATCT 
TTCCTGCAGC GTCTGGTCAA CATGGCGGAT GTCCTGATCT TTGCCACATC CCTAAACTTC 
GGCGAGTTGG AGGCGGAGAA GAACATGTCC AGTGGCGGCA TACTGCGTCA GTGCCTGCGA 
TTGGTTTGCA CCTGTGCGGT AAGGAATTGC TTGGAGTGCA AGGAGCGAAC GCGTTACAAT 
GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT GCAGCCCATT TGCAGGCCCT CATCCGCGGA 
GCTCAAGCAT CGCCCAAGAA CATTGTCGAG TCAATAACCG GCCAATTATC GCCTGTCAAG 
GATCCCGAGA AGCTGCTCCA GGACATGGAC GTCAATCGCC TGCGCGCAGT CATCTATCGC 
GATGTGGAGG AGACTAAGCA GGCGCAGTTC CTCTCGCTGG CGATAGTGTA CTTCATATCG 
GTGCTGATGG TGTCCAAGTA TCGTGATATC CTGGAGCCGC CGGCAGAGCC GCAAATCCAG 
CGTCAATCGC CAGTGCTGCA ACGCACGGCA GGCGGCGAAG CCGCCAGTGC GCGTCCTTTG 
TTCCCCCAAT GGTCGCATCA TGTTTACCCG CAATTCCTGC CCGAATCGCA CCAGAATCAC 
AACAGCAACA TGCAACACCA GCAGCAGCAG CAGCAGCAAC AGCAACATCA GCAGCAGCAA 
CACTACTACC AGCAACAGCA GCAACAGCAG GTTGTCCACA ATCATAGCCA TCATCACATG 
ACTGCCGCTT ACTACCAACA GCAGCAACAC CAGCAAGTTG CAGCAGGACA GCAGCAACAT 
TCGCCCACTC CTCTGGCCAC ACATTCGACC AGCTCCTCGG CCAGTTCGAC GGCCACATCT 
CAGCCAGCGT CGAGCTCCTC GCTCTCCAGC CTGGCATCCC AAAGTCAGCA GCAATCGCAT 
CGCCAGTTGC ACAAGCAGCA ACAGCAGCAG CAGCAGCAGC AGCAGCAACA ACAGCCACAT 
TACCATCCAC ATCAGCCGCA CTATGGACTG ATCAATGGGC ACCAGCAACA TCCGCAGCTA 
AATGGTAAAC ATTATGCGGA AAATGGATCG ACAGCGGGAT ACCATCCGCA TTCGCATCCA 
CATCCACACG GTGGTTATAT GCGAAATGGT GATACGTCGA CATTGCAACA GAATGGTATG 
CCGGACTATC AGGCAGTGGG TCTGATGAAT GGTCATGGCT CTGGTGGAAC GGGTAATAAC 
AATAACTCCA GCCTGATGAA CAACATGCGA AATCTGCGAA ATGGCGGAGG AGCTAACTCA 
TCATCTTCGC CACCGGGAAG CCATCAAGTT ATCCAAGGTG TTGCAACAGC GGGAGCTGTT 
AATGCAAATG CTGCTGTTGG TGTTGGCATG GGCGGAGCCG TTGGTGTGGG TATGGGGGGT 
GTGGCAGGTG GTTTGGATGG AGGCGTGGTC TACAAGACGA GCGCCATAAA TAATAATTAC 
CGCTACAATG GACGCAACGC ATCCGCTGGA ACAGGTGGTC GTCAAATCCA GGATAGTGAC 
TACGAAATAA TTGTCGTCGA TGAGAACAAT CCATCGGTTT TGGCGGATAA TGATTCACAT 
TCCAGTGGAC CACCATCCAT AAAGAGCGTT GACTCGGATG TGGGCTCCCT GAACATGAAC 
TCCACGGAAA ACGAAGTGCC CGAGGTGGAG TCATCCAGCG AGATTCTAAT CGATGACCAC 
AAACCGAGTC ACTCGAACGA CGAAAGCTGG ACGGATGTGA ATCTCAATGA GGATGCTGCC 
GTTCAGGCGG CAAGTGCCGG AATAGTTGTA GGTTTGGTGG ATAACAGAGG TAATGTTATA 
TCCGACAAAC ACGATCCGTC CCATCACAAC CAACAACAGC AGCAGCAGGC GGGAATTATT 
GGTCAGCAGC AGCAACATGG ATCACTTGGT CATTCGGAGC GGGGTGACAA ACCCGATTCG 
GAGATTTCGG TGGTGCGAGT GCCTGATGGT TATGGTGGTG CTGGTTCCGG TGGTGGCGTG 
AATTCCGGAC AAGGTCAGGG CGTTCCATCC AATCAGCGTC CTCGTCCCGA GGAGTTGCCC 
ATGAAGGCGC CCGCCTTGGT GGCCCAACTG CCGCTGACCA CACCTTCGCG AGAGGCCAGT 
CTCACCCAGA AGCTGGAAAT CGCATTGGGG CCAGTGTGTC CGCTGCTCCG TGAAATCATG 
GTGGACTTTG CGCCGTTCCT TTCGAAAACC CTAGTCGGCT CCCATGGCCA GGAATTGCTG 
ATGGAGGGCA AGGGTCTGAC GACCTTTAAG AACTCGCATT CCGTCGTAGA GCTGGTCATG 
TTGCTCTGCT CCCAGGAGTG GCAAAATAGC CTACAAAAGC ACGCAGGATT AGCCTTTATT 
GAGCTAATCA ATGAGGGTCG ACTGCTTTCG CACGCCATGA AGGATCACAT TGTTCGGGTG 
GCCAATGAGG CGGAGTTCAT CCTCAATAGA ATGCGAGCGG ATGATGTCCT CAAGCATGCC 
GACTTTGAAT CGCAATGTGC ACAGACACTT TTGGAGCGCA GGGAGGAGGA GCGAATGTGC 
GATCACTTGA TAACCGCCGC ACGTCGCAGA GACAATGTGA TTGCCAGCCG ACTGCTGGAG 
AAGGTACGAA ACATCATGTG TAATCGCCAC GGAGCCTGGG GTGATTCCAG CTCCACATCC 
AGTGGTGGCG CCATCGTTGG AGCGGTGCAA AAGAGTCCCT ACTGGAAGCT GGATGCCTGG 
GAGGATGATG CCAGACGTCG GAAACGGATG GTGCAAAATC CGCGCGGATC CTCGCATCCA 
CAGGCCACAC TGAAGGCGGC TTTGGAAAAT GGCGGACCTG AAGATGCCAT CCTGCAGACT 
CGCGACGAGT TCCACACCCA AATAGCCGTC TCGCGGACTC ATCCATCCGG TCAGCACAAT 
GGTGAGCTAC TGGACGACGC CGAGCTCTTG ATTGAGGATC GGGAGTTGGA TCTGGATCTC 
ACGGGTCCCG TCAACATTAG CACCAAGGCG AGATTGATAG CCCCCGGTCT GGTGGCACCC 
GGCACCGTCT CAATAACCAG CACTGAAATG TTCTTTGAGG TGGATGAAGA GCATCCCGAG 
TTCCAGAAGA TCGATGGCGA AGTTCTTAAG TACTGTGACC ATTTGCACGG CAAATGGTAC 
TTCTCCGAAG TGAGGGCCAT CTTCTCGAGG CGTTATCTCC TGCAAAATGT GGCCCTCGAG 
ATCTTTTTGG CCAGCCGTAC GTCCATATTG TTTGCCTTTC CCGATCAGCA TACGGTCAAG 
AAGGTAATCA AGGCCCTGCC CCGTGTGGGA GTGGGCATCA AGTATGGAAT ACCGCAGACG 
AGGAGGGCCT CAATGATGTC GCCCCGTCAA CTGATGCGCA ATTCGAACAT GACCCAAAAG 
TGGCAGCGTC GCGAGATTAG CAACTTTGAG TATCTTATGT TCCTGAACAC GATCGCCGGC 
AGGACGTACA ACGATCTAAA TCAGTATCCC ATCTTTCCGT GGGTGCTGAC CAACTATGAG 
TCCAAGGATT TGGATCTCAG CCTGCCGTCA AACTACAGGG ATCTGTCGAA ACCGATTGGC 
GCCCTGAATC CATCGCGCAG AGCCTATTTC GAGGAGCGTT ACGAGAGCTG GGACAGTGAC 
ACCATACCGC CCTTCCACTA TGGTACGCAT TATTCCACGG CAGCATTTAC GCTGAACTGG 
TTGGTGCGCG TGGAACCGTT TACCACCATG TTCCTGGCCC TGCAGGGCGG CAAATTTGAT 
TATCCGGACA GGCTGTTCAG CTCGGTGTCG CTGTCCTGGA AGAATTGCCA ACGTGATACA 
TCGGATGTGA AGGAACTGAT ACCAGAATGG TATTTCCTGC CCGAAATGTT TTACAATTCA 
TCAGGCTATC GACTGGGTCA TCGCGAGGAT GGTGCCCTAG TGGATGACAT TGAACTACCA 
CCGTGGGCCA AGAGCCCCGA AGAATTCGTC CGCATCAATC GCATGGCACT GGAGTCGGAG 
TTCGTGTCCT GCCAGCTGCA CCAGTGGATC GATTTGATCT TTGGCTACAA GCAACGCGGT 
CCCGAGGCCA TTAGGGCAAC CAACGTGTTC TACTATTTGA CATATGAGGG CAGCGTTGAC 
TTGGATGGCG TTTTGGATCC GGTGATGCGC GAAGCCGTCG AGAATCAAAT CCGCAACTTT 
GGCCAAACAC CTAGTCAACT GCTAATGGAA CCGCATCCGC CGCGCAGCTC TGCGATGCAT 
CTATCGCCTA TGATGTTCAG TGCGATGCCG GAGGATCTCT GCCAGATGCT CAAGTTCTAT 
CAGAACTCAC CAGTCATTCA CATCTCGGCC AACACCTATC CGCAACTGTC GTTGCCATCT 
GTGGTCACGG TAACGGCGGG TCATCAGTTT GCGGTGAATC GATGGAACTG CAACTACACC 
GCCTCCGTCC AGAGTCCCAG CTACGCCGAA TCGCCCCAAT CGCCGGGTTC CAATCAACCA 
CTGACCATCG ATCCGGTTCT GGCTGTTCAT GGCACCAACA ACAATAGCAA TGCGGCCAGC 
CGACGTCATT TGGGCGATAA CTTTAGCCAG ATGCTAAAGA TACGCTCCAA CTGCTTTGTT 
ACCACAGTGG ACAGTCGGTT CCTAATCGCA TGCGGATTCT GGGACAACAG TTTCCGCGTC 
TTTGCCACCG AAACGGCGAA AATCGTGCAG ATTGTATTCG GTCACTTTGG TGTGGTAACG 
TGCATGGCAC GTTCCGAGTG CAACATCACA TCAGACTGCT ACATCGCCTC CGGATCGGCG 
GACTGCACGG TGCTTCTGTG GCACTGGAAT GCCCGAACCC AGAGCATTGT GGGCGAGGGC 
GATGTGCCCA CACCACGGGC CACCTTGACG GGTCACGAAC AGGCCGTGAC ATCGGTGGTG 
ATTAGTGCCG AGTTGGGTCT GGTTGTGTCT GGATCATCAA ATGGACCCGT ACTTATCCAT 
ACGACTTTTG GCGACCTGCT GCGCTCACTG GATCCACCAG CGGAGTTCCA TTCCCCGGAA 
CTGATAACCA TGTCCCGCGA GGGTTTCATT GTCATCAACT ACGATAAGGG CAATGTGGCC 
GCCTACACCA TCAATGGCAA GAAACTGCGC CACGAGACGC ACAACGACAA TCTACAGTGC 
ATGCTACTGT CGCGCGATGG TGAATACCTG ATGACCGCCG GTGATCGCGG CATCGTGGAG 
GTGTGGCGCA CCTTCAACCT AGCACCACTT TATGCCTTCC CCGCATGCAA CGCGGGCATC 
CGATCTTTGG CTCTCACCCA CGATCAGAAA TACCTTCTGG CCGGACTTTC GACGGGCTCG 
ATCATAGTAT TCCACATCGA CTTCAATCGC TGGCACCACG AGTACCAGCA GCGCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036261.3
CDS	777..11936
Translation	MADIMRPPYSEIKRPDEIVRMTTADNLKFAVLIGLIEVGQVTNREVVNTVLHLLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSHFQLLPATLGASALSGGSGSGTGSGTGNDASAAAAAAVAAGQQQQLQLQFQILAAEQEARAIDWSDEKLDLNAAFVKIRAVLTARNAVTLAGSSSTGTTAVATAAAAAAAAGAGAGTTAAATSAAAAAAATQNENDAAVGQQQHATHHHATAATGSADDPLGHLPTGNASSSSSSFEQLRRMSSVSSLNSMVGSADTEEVNQLKAVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGEAASARPLFPQWSHHVYPQFLPESHQNHNSNMQHQQQQQQQQQHQQQQHYYQQQQQQQVVHNHSHHHMTAAYYQQQQHQQVAAGQQQHSPTPLATHSTSSSASSTATSQPASSSSLSSLASQSQQQSHRQLHKQQQQQQQQQQQQQPHYHPHQPHYGLINGHQQHPQLNGKHYAENGSTAGYHPHSHPHPHGGYMRNGDTSTLQQNGMPDYQAVGLMNGHGSGGTGNNNNSSLMNNMRNLRNGGGANSSSSPPGSHQVIQGVATAGAVNANAAVGVGMGGAVGVGMGGVAGGLDGGVVYKTSAINNNYRYNGRNASAGTGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_001042796.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant L, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042796.3

ORF Insert Sequence:

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGCTG 
CTGGATCACG TCCTCTTCAA TCCAGCCCTG TGGATATACA CACCCGCCAA TGTCCAGGCG 
CGACTATACT CCTACTTGGC CACCGAGTTC CTCTCGGACA CGCAGATCTA CAGCAATGTG 
AGGAGGGTCA GCACGGTACT GCAGACAGTG CACACCCTAA AGTACTACTA CTGGGTGGTC 
AATCCACGGG CTAAGAGCGG CATCATTCCC AAGGGACTGG ATGGACCTCG TCCGGCTCAA 
AAGGACATCC TGGCCATCCG GGCATACATC CTGCTGTTCC TGAAGCAGCT CATAATGATC 
GGCAATGGCG TGAAGGAGGA CGAGCTGCAG AGCATACTCA ACTATCTGAC CACCATGCAC 
GAGGACGAGA ACCTGCACGA CGTGCTGCAG ATGCTCATCT CGCTGATGTC GGAGCATCCC 
AGCTCCATGG TACCTGCCTT TGATGTGAAG CACGGCGTGC GCAGCATCTT CAAGTTGCTG 
GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC 
CGCAGCACCC ACAAACGCAA GTACGATGTG ATGTCACCAC ACAATCTGTA CACCCTTTTA 
GCGGAGCGAC TGCTTCTCTA TGAGGAATCA CTGTCCCTGC CCACATACAA TGTTCTCTAT 
GAGATCATGA CGGAGCACAT CTCGCAGCAG ATTCTATACA CACGCCATCC GGAACCAGAG 
AGTCACTACC GTCTGGAGAA TCCAATGATC CTCAAGGTGG TGGCCACCCT GATCCGGCAA 
TCAAAGCAGA CGGAATCGCT GATCGACGTT AAAAAGCTAT TCCTGCAGGA TATGACCCTG 
TTGTGCAATA GCAATCGGGA GAACCGGCGC ACAGTGCTCC AGATGTCCGT GTGGCAGGAG 
TGGCTCATTG CGATGGCGTA CATTCATCCA AAGAGCAGCG AGGAGCAGAA GATAAGCGAC 
ATGGTGTATT CCCTATTCCG CATGCTGCTC CACCATGCCA TTAAGCATGA ATACGGTGGC 
TGGAGAGTTT GGGTAGATAC CCTGGCAATC GTCCACTCCA AGGTGTCTTA CGAGGAGTTT 
AAGCTCCAGT TTGCCCAGAT GTACGAGCAC TACGAGCGCC AGCGAACGGA CAATATCACA 
GATCCTGCCC TGCGTCAGGC TAGACCCATC AGCACGATCA GTGGTTGGGA GCGAGAGGAG 
CTGCATCAGC AGCAGAACGG TGGATCTGCA GCAGCAGTGG CTACCAATCA AACGGCAGCT 
GTTAAAGGTT CTGTTTCCAT TGCCTCACTG GAAGATGTGC CACCGGTGGT TGAGGAAGAG 
GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG ATTCAAGAGG GTCCTATAAC CGAAGAAACC 
GAACAAAAAT CGGTGATAGC CAACATTTCC GACGTCTACA ATGAGCAGCT CAAAACCGAT 
GCCACGTGCA ATGGCAATTT GGAGGATGTC AAAGAGGAGG AGCCTGTACA ACAGCAAATC 
GGAGACCTGG AGAAGCAACC AGAGCCATCT ACTCCTTTGG GAGCACTCAG GGAAACCCTA 
CAACTTGGTG ATGATATGGA TGTGGAAGAA CTGGAGCTGG CTACCGCCAA GGATGCCCTC 
AATGCGGAAC AGCATGTGTC GCGAGTTCTT CAGGCTTCCG AAGCGGCACT TAACGATTGC 
AAAATGGCAG TCGATGATGT CCTGCAGGAG TCATCCTCCG TCCTCAAGGA CGAGGAGATC 
GAACTGGCCG TCAACGAGGT TGTTCAGGGT GTGCTCAATA ACGAGAAGAA AACTCAATCC 
CAGGACAACA AGGATAACAA GGAACAGCCG GGCGAACAGG ATGTAAACGT CAGTCTCCTA 
AACAGCAAGA ATCTGCTCAA CAATAATAAT AATAACAATA ACAACAGTCC GAGTCCAACG 
CCCACAACGG CGACTGCGAC GGCGGAGACG GAAGCGGAAA CAGAAGTCAA TGCCAATGAG 
ATCGTGAGCA GCACAGAGGC GCCCAAGGCG GAAACGGAAA CGAGCGTAGC ACCGGAAGTA 
GAAACACCGG AAACGGCCAA GCCAAGCCCC ATTGTCCCCA GCCCCGTATT AGCAACCAAT 
CAAAAGACTG AAGATGCAGC CAACAAGCTG AACAACAACG AGAAGCTGGC CGAGATCAGC 
GCCAGTCCCG AGCCACCCAT TGTCGTGGAA ACGCCAGAAG CTGATCTTCT TCAGCTTTCC 
GATTCCGAAA CAAAACCGAA TAAGGAAACC GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT 
AGAGATATTG TCGAGCAACT CATCGACAAG GTCATCGATG CCACGGAAGC GGAATCGGCC 
AGCGAAACCA AAACGGAAAC GAATAATAAT GAAATACCCA AGAAGGAGAA GCAGACGTCA 
GAAGAGCCTG AGGATGTGGA AACTGCAGAG ACTTTGGCCG CTGCCGCCAA GGAAATTGTC 
CAAGAGGTTG TGGAAGCAGC TCTCGTAATG GTCCAAGAGG AGAGCACTCA AGAGAAGCCA 
GAAAAGGGGG CGAACTCCGA AGAGGAGAAA AACGAAATTG GCAAGGAAGA GATTCTTCTG 
CAGCTGGAGG AAAAACCAGC GTCTACTGAA GTGCAGGAAA CCAAGATCGA AGGAGATCTA 
AAGAAACCAG AAGATCCAAA AGGTCACAGC TCTGTAGAAC CAAAGACCCC GAACCTTGAG 
GAACCGAAAC CCCAGGAAAC TGAACAACAA AAGTCGCAGG AGGTCGCCGA AGAACTACCT 
CAAAAACCAG AGGAGCAGGT GGTGGCCATT GTCAACCAAG TCCTGGACAC TCTGGTAGAC 
GATACGGTCA AGGCAGTGGC AGCCGAGCAA ACCACTCAAA CATCGCCAGC CCCCGAAGAG 
CAATCGCCAC AAATACTGGC AATGGAATCA CCAGCAACGT CGGTGCGGGT CAAACCCACT 
GAGGTGGATT CCACCACCCA GACTACGCCG AAGAACGAGG CTGGATCCAG TCTATTGGTT 
GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT GACGCCCAGC AATCAGCCGG CATGACCATC 
GAAGATGAGG ATTACTCTAA TCAGCAGGCA GCTGCGGCAG TTGAAAATGC CAACAGCAGC 
CAATTGGATG CCAATCATTA TGGTCCCGGT AATCCAGAAT CCAAACAACA GCAACAGCGC 
TCCAAATCGG GATCGACCCG ACCTATGTTC AGTCCGGGAC CTACACGTCC GCCCTTCCGA 
ATACCGGAAT TCAAGTGGTC CTACATCCAT CAGCGATTGC TCAGCGATGT CCTCTTTTCG 
CTGGAAACGG ACATTCAGGT GTGGCGCAGT CATTCGACCA AAAGCGTCCT GGACTTTGTC 
AACTCCAGCG AGAATGCAAT TTTTGTGGTG AACACGGTGC ATTTAATATC GCAGCTAGCG 
GATAATCTGA TTATTGCCTG CGGAGGATTG CTGCCTCTTT TGGCCAGCGC CACGTCACCC 
AATTCCGAAT TGGATGTGCT GGAGCCAACG CAAGGCATGC CTTTGGAGGT GGCCGTATCT 
TTCCTGCAGC GTCTGGTCAA CATGGCGGAT GTCCTGATCT TTGCCACATC CCTAAACTTC 
GGCGAGTTGG AGGCGGAGAA GAACATGTCC AGTGGCGGCA TACTGCGTCA GTGCCTGCGA 
TTGGTTTGCA CCTGTGCGGT AAGGAATTGC TTGGAGTGCA AGGAGCGAAC GCGTTACAAT 
GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT GCAGCCCATT TGCAGGCCCT CATCCGCGGA 
GCTCAAGCAT CGCCCAAGAA CATTGTCGAG TCAATAACCG GCCAATTATC GCCTGTCAAG 
GATCCCGAGA AGCTGCTCCA GGACATGGAC GTCAATCGCC TGCGCGCAGT CATCTATCGC 
GATGTGGAGG AGACTAAGCA GGCGCAGTTC CTCTCGCTGG CGATAGTGTA CTTCATATCG 
GTGCTGATGG TGTCCAAGTA TCGTGATATC CTGGAGCCGC CGGCAGAGCC GCAAATCCAG 
CGTCAATCGC CAGTGCTGCA ACGCACGGCA GGCGGCGAAG CCGCCAGTGC GCGTCCTTTG 
TTCCCCCAAT GGTCGCATCA TGTTTACCCG CAATTCCTGC CCGAATCGCA CCAGAATCAC 
AACAGCAACA TGCAACACCA GCAGCAGCAG CAGCAGCAAC AGCAACATCA GCAGCAGCAA 
CACTACTACC AGCAACAGCA GCAACAGCAG GTTGTCCACA ATCATAGCCA TCATCACATG 
ACTGCCGCTT ACTACCAACA GCAGCAACAC CAGCAAGTTG CAGCAGGACA GCAGCAACAT 
TCGCCCACTC CTCTGGCCAC ACATTCGACC AGCTCCTCGG CCAGTTCGAC GGCCACATCT 
CAGCCAGCGT CGAGCTCCTC GCTCTCCAGC CTGGCATCCC AAAGTCAGCA GCAATCGCAT 
CGCCAGTTGC ACAAGCAGCA ACAGCAGCAG CAGCAGCAGC AGCAGCAACA ACAGCCACAT 
TACCATCCAC ATCAGCCGCA CTATGGACTG ATCAATGGGC ACCAGCAACA TCCGCAGCTA 
AATGGTAAAC ATTATGCGGA AAATGGATCG ACAGCGGGAT ACCATCCGCA TTCGCATCCA 
CATCCACACG GTGGTTATAT GCGAAATGGT GATACGTCGA CATTGCAACA GAATGGTATG 
CCGGACTATC AGGCAGTGGG TCTGATGAAT GGTCATGGCT CTGGTGGAAC GGGTAATAAC 
AATAACTCCA GCCTGATGAA CAACATGCGA AATCTGCGAA ATGGCGGAGG AGCTAACTCA 
TCATCTTCGC CACCGGGAAG CCATCAAGTT ATCCAAGGTG TTGCAACAGC GGGAGCTGTT 
AATGCAAATG CTGCTGTTGG TGTTGGCATG GGCGGAGCCG TTGGTGTGGG TATGGGGGGT 
GTGGCAGGTG GTTTGGATGG AGGCGTGGTC TACAAGACGA GCGCCATAAA TAATAATTAC 
CGCTACAATG GACGCAACGC ATCCGCTGGA ACAGGTGGTC GTCAAATCCA GGATAGTGAC 
TACGAAATAA TTGTCGTCGA TGAGAACAAT CCATCGGTTT TGGCGGATAA TGATTCACAT 
TCCAGTGGAC CACCATCCAT AAAGAGCGTT GACTCGGATG TGGGCTCCCT GAACATGAAC 
TCCACGGAAA ACGAAGTGCC CGAGGTGGAG TCATCCAGCG AGATTCTAAT CGATGACCAC 
AAACCGAGTC ACTCGAACGA CGAAAGCTGG ACGGATGTGA ATCTCAATGA GGATGCTGCC 
GTTCAGGCGG CAAGTGCCGG AATAGTTGTA GGTTTGGTGG ATAACAGAGG TAATGTTATA 
TCCGACAAAC ACGATCCGTC CCATCACAAC CAACAACAGC AGCAGCAGGC GGGAATTATT 
GGTCAGCAGC AGCAACATGG ATCACTTGGT CATTCGGAGC GGGGTGACAA ACCCGATTCG 
GAGATTTCGG TGGTGCGAGT GCCTGATGGT TATGGTGGTG CTGGTTCCGG TGGTGGCGTG 
AATTCCGGAC AAGGTCAGGG CGTTCCATCC AATCAGCGTC CTCGTCCCGA GGAGTTGCCC 
ATGAAGGCGC CCGCCTTGGT GGCCCAACTG CCGCTGACCA CACCTTCGCG AGAGGCCAGT 
CTCACCCAGA AGCTGGAAAT CGCATTGGGG CCAGTGTGTC CGCTGCTCCG TGAAATCATG 
GTGGACTTTG CGCCGTTCCT TTCGAAAACC CTAGTCGGCT CCCATGGCCA GGAATTGCTG 
ATGGAGGGCA AGGGTCTGAC GACCTTTAAG AACTCGCATT CCGTCGTAGA GCTGGTCATG 
TTGCTCTGCT CCCAGGAGTG GCAAAATAGC CTACAAAAGC ACGCAGGATT AGCCTTTATT 
GAGCTAATCA ATGAGGGTCG ACTGCTTTCG CACGCCATGA AGGATCACAT TGTTCGGGTG 
GCCAATGAGG CGGAGTTCAT CCTCAATAGA ATGCGAGCGG ATGATGTCCT CAAGCATGCC 
GACTTTGAAT CGCAATGTGC ACAGACACTT TTGGAGCGCA GGGAGGAGGA GCGAATGTGC 
GATCACTTGA TAACCGCCGC ACGTCGCAGA GACAATGTGA TTGCCAGCCG ACTGCTGGAG 
AAGGTACGAA ACATCATGTG TAATCGCCAC GGAGCCTGGG GTGATTCCAG CTCCACATCC 
AGTGGTGGCG CCATCGTTGG AGCGGTGCAA AAGAGTCCCT ACTGGAAGCT GGATGCCTGG 
GAGGATGATG CCAGACGTCG GAAACGGATG GTGCAAAATC CGCGCGGATC CTCGCATCCA 
CAGGCCACAC TGAAGGCGGC TTTGGAAAAT GGCGGACCTG AAGATGCCAT CCTGCAGACT 
CGCGACGAGT TCCACACCCA AATAGCCGTC TCGCGGACTC ATCCATCCGG TCAGCACAAT 
GGTGAGCTAC TGGACGACGC CGAGCTCTTG ATTGAGGATC GGGAGTTGGA TCTGGATCTC 
ACGGGTCCCG TCAACATTAG CACCAAGGCG AGATTGATAG CCCCCGGTCT GGTGGCACCC 
GGCACCGTCT CAATAACCAG CACTGAAATG TTCTTTGAGG TGGATGAAGA GCATCCCGAG 
TTCCAGAAGA TCGATGGCGA AGTTCTTAAG TACTGTGACC ATTTGCACGG CAAATGGTAC 
TTCTCCGAAG TGAGGGCCAT CTTCTCGAGG CGTTATCTCC TGCAAAATGT GGCCCTCGAG 
ATCTTTTTGG CCAGCCGTAC GTCCATATTG TTTGCCTTTC CCGATCAGCA TACGGTCAAG 
AAGGTAATCA AGGCCCTGCC CCGTGTGGGA GTGGGCATCA AGTATGGAAT ACCGCAGACG 
AGGAGGGCCT CAATGATGTC GCCCCGTCAA CTGATGCGCA ATTCGAACAT GACCCAAAAG 
TGGCAGCGTC GCGAGATTAG CAACTTTGAG TATCTTATGT TCCTGAACAC GATCGCCGGC 
AGGACGTACA ACGATCTAAA TCAGTATCCC ATCTTTCCGT GGGTGCTGAC CAACTATGAG 
TCCAAGGATT TGGATCTCAG CCTGCCGTCA AACTACAGGG ATCTGTCGAA ACCGATTGGC 
GCCCTGAATC CATCGCGCAG AGCCTATTTC GAGGAGCGTT ACGAGAGCTG GGACAGTGAC 
ACCATACCGC CCTTCCACTA TGGTACGCAT TATTCCACGG CAGCATTTAC GCTGAACTGG 
TTGGTGCGCG TGGAACCGTT TACCACCATG TTCCTGGCCC TGCAGGGCGG CAAATTTGAT 
TATCCGGACA GGCTGTTCAG CTCGGTGTCG CTGTCCTGGA AGAATTGCCA ACGTGATACA 
TCGGATGTGA AGGAACTGAT ACCAGAATGG TATTTCCTGC CCGAAATGTT TTACAATTCA 
TCAGGCTATC GACTGGGTCA TCGCGAGGAT GGTGCCCTAG TGGATGACAT TGAACTACCA 
CCGTGGGCCA AGAGCCCCGA AGAATTCGTC CGCATCAATC GCATGGCACT GGAGTCGGAG 
TTCGTGTCCT GCCAGCTGCA CCAGTGGATC GATTTGATCT TTGGCTACAA GCAACGCGGT 
CCCGAGGCCA TTAGGGCAAC CAACGTGTTC TACTATTTGA CATATGAGGG CAGCGTTGAC 
TTGGATGGCG TTTTGGATCC GGTGATGCGC GAAGCCGTCG AGAATCAAAT CCGCAACTTT 
GGCCAAACAC CTAGTCAACT GCTAATGGAA CCGCATCCGC CGCGCAGCTC TGCGATGCAT 
CTATCGCCTA TGATGTTCAG TGCGATGCCG GAGGATCTCT GCCAGATGCT CAAGTTCTAT 
CAGAACTCAC CAGTCATTCA CATCTCGGCC AACACCTATC CGCAACTGTC GTTGCCATCT 
GTGGTCACGG TAACGGCGGG TCATCAGTTT GCGGTGAATC GATGGAACTG CAACTACACC 
GCCTCCGTCC AGAGTCCCAG CTACGCCGAA TCGCCCCAAT CGCCGGGTTC CAATCAACCA 
CTGACCATCG ATCCGGTTCT GGCTGTTCAT GGCACCAACA ACAATAGCAA TGCGGCCAGC 
CGACGTCATT TGGGCGATAA CTTTAGCCAG ATGCTAAAGA TACGCTCCAA CTGCTTTGTT 
ACCACAGTGG ACAGTCGGTT CCTAATCGCA TGCGGATTCT GGGACAACAG TTTCCGCGTC 
TTTGCCACCG AAACGGCGAA AATCGTGCAG ATTGTATTCG GTCACTTTGG TGTGGTAACG 
TGCATGGCAC GTTCCGAGTG CAACATCACA TCAGACTGCT ACATCGCCTC CGGATCGGCG 
GACTGCACGG TGCTTCTGTG GCACTGGAAT GCCCGAACCC AGAGCATTGT GGGCGAGGGC 
GATGTGCCCA CACCACGGGC CACCTTGACG GGTCACGAAC AGGCCGTGAC ATCGGTGGTG 
ATTAGTGCCG AGTTGGGTCT GGTTGTGTCT GGATCATCAA ATGGACCCGT ACTTATCCAT 
ACGACTTTTG GCGACCTGCT GCGCTCACTG GATCCACCAG CGGAGTTCCA TTCCCCGGAA 
CTGATAACCA TGTCCCGCGA GGGTTTCATT GTCATCAACT ACGATAAGGG CAATGTGGCC 
GCCTACACCA TCAATGGCAA GAAACTGCGC CACGAGACGC ACAACGACAA TCTACAGTGC 
ATGCTACTGT CGCGCGATGG TGAATACCTG ATGACCGCCG GTGATCGCGG CATCGTGGAG 
GTGTGGCGCA CCTTCAACCT AGCACCACTT TATGCCTTCC CCGCATGCAA CGCGGGCATC 
CGATCTTTGG CTCTCACCCA CGATCAGAAA TACCTTCTGG CCGGACTTTC GACGGGCTCG 
ATCATAGTAT TCCACATCGA CTTCAATCGC TGGCACCACG AGTACCAGCA GCGCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rg ( NM_001144685.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138157.2 Drosophila melanogaster rugose (rg), transcript variant O, mRNA.

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CloneID	OFa22219
Clone ID Related Accession (Same CDS sequence)	NM_001144685.2
Accession Version	NM_001144685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform O
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144685.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGCTG 
CTGGATCACG TCCTCTTCAA TCCAGCCCTG TGGATATACA CACCCGCCAA TGTCCAGGCG 
CGACTATACT CCTACTTGGC CACCGAGTTC CTCTCGGACA CGCAGATCTA CAGCAATGTG 
AGGAGGGTCA GCACGGTACT GCAGACAGTG CACACCCTAA AGTACTACTA CTGGGTGGTC 
AATCCACGGG CTAAGAGCGG CATCATTCCC AAGGGACTGG ATGGACCTCG TCCGGCTCAA 
AAGGACATCC TGGCCATCCG GGCATACATC CTGCTGTTCC TGAAGCAGCT CATAATGATC 
GGCAATGGCG TGAAGGAGGA CGAGCTGCAG AGCATACTCA ACTATCTGAC CACCATGCAC 
GAGGACGAGA ACCTGCACGA CGTGCTGCAG ATGCTCATCT CGCTGATGTC GGAGCATCCC 
AGCTCCATGG TACCTGCCTT TGATGTGAAG CACGGCGTGC GCAGCATCTT CAAGTTGCTG 
GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC 
CGCAGCACCC ACAAACGCAA GTACGATGTG ATGTCACCAC ACAATCTGTA CACCCTTTTA 
GCGGAGCGAC TGCTTCTCTA TGAGGAATCA CTGTCCCTGC CCACATACAA TGTTCTCTAT 
GAGATCATGA CGGAGCACAT CTCGCAGCAG ATTCTATACA CACGCCATCC GGAACCAGAG 
AGTCACTACC GTCTGGAGAA TCCAATGATC CTCAAGGTGG TGGCCACCCT GATCCGGCAA 
TCAAAGCAGA CGGAATCGCT GATCGACGTT AAAAAGCTAT TCCTGCAGGA TATGACCCTG 
TTGTGCAATA GCAATCGGGA GAACCGGCGC ACAGTGCTCC AGATGTCCGT GTGGCAGGAG 
TGGCTCATTG CGATGGCGTA CATTCATCCA AAGAGCAGCG AGGAGCAGAA GATAAGCGAC 
ATGGTGTATT CCCTATTCCG CATGCTGCTC CACCATGCCA TTAAGCATGA ATACGGTGGC 
TGGAGAGTTT GGGTAGATAC CCTGGCAATC GTCCACTCCA AGGTGTCTTA CGAGGAGTTT 
AAGCTCCAGT TTGCCCAGAT GTACGAGCAC TACGAGCGCC AGCGAACGGA CAATATCACA 
GATCCTGCCC TGCGTCAGGC TAGACCCATC AGCACGATCA GTGGTTGGGA GCGAGAGGAG 
CTGCATCAGC AGCAGAACGG TGGATCTGCA GCAGCAGTGG CTACCAATCA AACGGCAGCT 
GTTAAAGGTT CTGTTTCCAT TGCCTCACTG GAAGATGTGC CACCGGTGGT TGAGGAAGAG 
GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG ATTCAAGAGG GTCCTATAAC CGAAGAAACC 
GAACAAAAAT CGGTGATAGC CAACATTTCC GACGTCTACA ATGAGCAGCT CAAAACCGAT 
GCCACGTGCA ATGGCAATTT GGAGGATGTC AAAGAGGAGG AGCCTGTACA ACAGCAAATC 
GGAGACCTGG AGAAGCAACC AGAGCCATCT ACTCCTTTGG GAGCACTCAG GGAAACCCTA 
CAACTTGGTG ATGATATGGA TGTGGAAGAA CTGGAGCTGG CTACCGCCAA GGATGCCCTC 
AATGCGGAAC AGCATGTGTC GCGAGTTCTT CAGGCTTCCG AAGCGGCACT TAACGATTGC 
AAAATGGCAG TCGATGATGT CCTGCAGGAG TCATCCTCCG TCCTCAAGGA CGAGGAGATC 
GAACTGGCCG TCAACGAGGT TGTTCAGGGT GTGCTCAATA ACGAGAAGAA AACTCAATCC 
CAGGACAACA AGGATAACAA GGAACAGCCG GGCGAACAGG ATGTAAACGT CAGTCTCCTA 
AACAGCAAGA ATCTGCTCAA CAATAATAAT AATAACAATA ACAACAGTCC GAGTCCAACG 
CCCACAACGG CGACTGCGAC GGCGGAGACG GAAGCGGAAA CAGAAGTCAA TGCCAATGAG 
ATCGTGAGCA GCACAGAGGC GCCCAAGGCG GAAACGGAAA CGAGCGTAGC ACCGGAAGTA 
GAAACACCGG AAACGGCCAA GCCAAGCCCC ATTGTCCCCA GCCCCGTATT AGCAACCAAT 
CAAAAGACTG AAGATGCAGC CAACAAGCTG AACAACAACG AGAAGCTGGC CGAGATCAGC 
GCCAGTCCCG AGCCACCCAT TGTCGTGGAA ACGCCAGAAG CTGATCTTCT TCAGCTTTCC 
GATTCCGAAA CAAAACCGAA TAAGGAAACC GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT 
AGAGATATTG TCGAGCAACT CATCGACAAG GTCATCGATG CCACGGAAGC GGAATCGGCC 
AGCGAAACCA AAACGGAAAC GAATAATAAT GAAATACCCA AGAAGGAGAA GCAGACGTCA 
GAAGAGCCTG AGGATGTGGA AACTGCAGAG ACTTTGGCCG CTGCCGCCAA GGAAATTGTC 
CAAGAGGTTG TGGAAGCAGC TCTCGTAATG GTCCAAGAGG AGAGCACTCA AGAGAAGCCA 
GAAAAGGGGG CGAACTCCGA AGAGGAGAAA AACGAAATTG GCAAGGAAGA GATTCTTCTG 
CAGCTGGAGG AAAAACCAGC GTCTACTGAA GTGCAGGAAA CCAAGATCGA AGGAGATCTA 
AAGAAACCAG AAGATCCAAA AGGTCACAGC TCTGTAGAAC CAAAGACCCC GAACCTTGAG 
GAACCGAAAC CCCAGGAAAC TGAACAACAA AAGTCGCAGG AGGTCGCCGA AGAACTACCT 
CAAAAACCAG AGGAGCAGGT GGTGGCCATT GTCAACCAAG TCCTGGACAC TCTGGTAGAC 
GATACGGTCA AGGCAGTGGC AGCCGAGCAA ACCACTCAAA CATCGCCAGC CCCCGAAGAG 
CAATCGCCAC AAATACTGGC AATGGAATCA CCAGCAACGT CGGTGCGGGT CAAACCCACT 
GAGGTGGATT CCACCACCCA GACTACGCCG AAGAACGAGG CTGGATCCAG TCTATTGGTT 
GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT GACGCCCAGC AATCAGCCGG CATGACCATC 
GAAGATGAGG ATTACTCTAA TCAGCAGGCA GCTGCGGCAG TTGAAAATGC CAACAGCAGC 
CAATTGGATG CCAATCATTA TGGTCCCGGT AATCCAGAAT CCAAACAACA GCAACAGCGC 
TCCAAATCGG GATCGACCCG ACCTATGTTC AGTCCGGGAC CTACACGTCC GCCCTTCCGA 
ATACCGGAAT TCAAGTGGTC CTACATCCAT CAGCGATTGC TCAGCGATGT CCTCTTTTCG 
CTGGAAACGG ACATTCAGGT GTGGCGCAGT CATTCGACCA AAAGCGTCCT GGACTTTGTC 
AACTCCAGCG AGAATGCAAT TTTTGTGGTG AACACGGTGC ATTTAATATC GCAGCTAGCG 
GATAATCTGA TTATTGCCTG CGGAGGATTG CTGCCTCTTT TGGCCAGCGC CACGTCACCC 
AATTCCGAAT TGGATGTGCT GGAGCCAACG CAAGGCATGC CTTTGGAGGT GGCCGTATCT 
TTCCTGCAGC GTCTGGTCAA CATGGCGGAT GTCCTGATCT TTGCCACATC CCTAAACTTC 
GGCGAGTTGG AGGCGGAGAA GAACATGTCC AGTGGCGGCA TACTGCGTCA GTGCCTGCGA 
TTGGTTTGCA CCTGTGCGGT AAGGAATTGC TTGGAGTGCA AGGAGCGAAC GCGTTACAAT 
GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT GCAGCCCATT TGCAGGCCCT CATCCGCGGA 
GCTCAAGCAT CGCCCAAGAA CATTGTCGAG TCAATAACCG GCCAATTATC GCCTGTCAAG 
GATCCCGAGA AGCTGCTCCA GGACATGGAC GTCAATCGCC TGCGCGCAGT CATCTATCGC 
GATGTGGAGG AGACTAAGCA GGCGCAGTTC CTCTCGCTGG CGATAGTGTA CTTCATATCG 
GTGCTGATGG TGTCCAAGTA TCGTGATATC CTGGAGCCGC CGGCAGAGCC GCAAATCCAG 
CGTCAATCGC CAGTGCTGCA ACGCACGGCA GGCGGCGGTG GTCGTCAAAT CCAGGATAGT 
GACTACGAAA TAATTGTCGT CGATGAGAAC AATCCATCGG TTTTGGCGGA TAATGATTCA 
CATTCCAGTG GACCACCATC CATAAAGAGC GTTGACTCGG ATGTGGGCTC CCTGAACATG 
AACTCCACGG AAAACGAAGT GCCCGAGGTG GAGTCATCCA GCGAGATTCT AATCGATGAC 
CACAAACCGA GTCACTCGAA CGACGAAAGC TGGACGGATG TGAATCTCAA TGAGGATGCT 
GCCGTTCAGG CGGCAAGTGC CGGAATAGTT GTAGGTTTGG TGGATAACAG AGGTAATGTT 
ATATCCGACA AACACGATCC GTCCCATCAC AACCAACAAC AGCAGCAGCA GGCGGGAATT 
ATTGGTCAGC AGCAGCAACA TGGATCACTT GGTCATTCGG AGCGGGGTGA CAAACCCGAT 
TCGGAGATTT CGGTGGTGCG AGTGCCTGAT GGTTATGGTG GTGCTGGTTC CGGTGGTGGC 
GTGAATTCCG GACAAGGTCA GGGCGTTCCA TCCAATCAGC GTCCTCGTCC CGAGGAGTTG 
CCCATGAAGG CGCCCGCCTT GGTGGCCCAA CTGCCGCTGA CCACACCTTC GCGAGAGGCC 
AGTCTCACCC AGAAGCTGGA AATCGCATTG GGGCCAGTGT GTCCGCTGCT CCGTGAAATC 
ATGGTGGACT TTGCGCCGTT CCTTTCGAAA ACCCTAGTCG GCTCCCATGG CCAGGAATTG 
CTGATGGAGG GCAAGGGTCT GACGACCTTT AAGAACTCGC ATTCCGTCGT AGAGCTGGTC 
ATGTTGCTCT GCTCCCAGGA GTGGCAAAAT AGCCTACAAA AGCACGCAGG ATTAGCCTTT 
ATTGAGCTAA TCAATGAGGG TCGACTGCTT TCGCACGCCA TGAAGGATCA CATTGTTCGG 
GTGGCCAATG AGGCGGAGTT CATCCTCAAT AGAATGCGAG CGGATGATGT CCTCAAGCAT 
GCCGACTTTG AATCGCAATG TGCACAGACA CTTTTGGAGC GCAGGGAGGA GGAGCGAATG 
TGCGATCACT TGATAACCGC CGCACGTCGC AGAGACAATG TGATTGCCAG CCGACTGCTG 
GAGAAGGTAC GAAACATCAT GTGTAATCGC CACGGAGCCT GGGGTGATTC CAGCTCCACA 
TCCAGTGGTG GCGCCATCGT TGGAGCGGTG CAAAAGAGTC CCTACTGGAA GCTGGATGCC 
TGGGAGGATG ATGCCAGACG TCGGAAACGG ATGGTGCAAA ATCCGCGCGG ATCCTCGCAT 
CCACAGGCCA CACTGAAGGC GGCTTTGGAA AATGGCGGAC CTGAAGATGC CATCCTGCAG 
ACTCGCGACG AGTTCCACAC CCAAATAGCC GTCTCGCGGA CTCATCCATC CGGTCAGCAC 
AATGGTGAGC TACTGGACGA CGCCGAGCTC TTGATTGAGG ATCGGGAGTT GGATCTGGAT 
CTCACGGGTC CCGTCAACAT TAGCACCAAG GCGAGATTGA TAGCCCCCGG TCTGGTGGCA 
CCCGGCACCG TCTCAATAAC CAGCACTGAA ATGTTCTTTG AGGTGGATGA AGAGCATCCC 
GAGTTCCAGA AGATCGATGG CGAAGTTCTT AAGTACTGTG ACCATTTGCA CGGCAAATGG 
TACTTCTCCG AAGTGAGGGC CATCTTCTCG AGGCGTTATC TCCTGCAAAA TGTGGCCCTC 
GAGATCTTTT TGGCCAGCCG TACGTCCATA TTGTTTGCCT TTCCCGATCA GCATACGGTC 
AAGAAGGTAA TCAAGGCCCT GCCCCGTGTG GGAGTGGGCA TCAAGTATGG AATACCGCAG 
ACGAGGAGGG CCTCAATGAT GTCGCCCCGT CAACTGATGC GCAATTCGAA CATGACCCAA 
AAGTGGCAGC GTCGCGAGAT TAGCAACTTT GAGTATCTTA TGTTCCTGAA CACGATCGCC 
GGCAGGACGT ACAACGATCT AAATCAGTAT CCCATCTTTC CGTGGGTGCT GACCAACTAT 
GAGTCCAAGG ATTTGGATCT CAGCCTGCCG TCAAACTACA GGGATCTGTC GAAACCGATT 
GGCGCCCTGA ATCCATCGCG CAGAGCCTAT TTCGAGGAGC GTTACGAGAG CTGGGACAGT 
GACACCATAC CGCCCTTCCA CTATGGTACG CATTATTCCA CGGCAGCATT TACGCTGAAC 
TGGTTGGTGC GCGTGGAACC GTTTACCACC ATGTTCCTGG CCCTGCAGGG CGGCAAATTT 
GATTATCCGG ACAGGCTGTT CAGCTCGGTG TCGCTGTCCT GGAAGAATTG CCAACGTGAT 
ACATCGGATG TGAAGGAACT GATACCAGAA TGGTATTTCC TGCCCGAAAT GTTTTACAAT 
TCATCAGGCT ATCGACTGGG TCATCGCGAG GATGGTGCCC TAGTGGATGA CATTGAACTA 
CCACCGTGGG CCAAGAGCCC CGAAGAATTC GTCCGCATCA ATCGCATGGC ACTGGAGTCG 
GAGTTCGTGT CCTGCCAGCT GCACCAGTGG ATCGATTTGA TCTTTGGCTA CAAGCAACGC 
GGTCCCGAGG CCATTAGGGC AACCAACGTG TTCTACTATT TGACATATGA GGGCAGCGTT 
GACTTGGATG GCGTTTTGGA TCCGGTGATG CGCGAAGCCG TCGAGAATCA AATCCGCAAC 
TTTGGCCAAA CACCTAGTCA ACTGCTAATG GAACCGCATC CGCCGCGCAG CTCTGCGATG 
CATCTATCGC CTATGATGTT CAGTGCGATG CCGGAGGATC TCTGCCAGAT GCTCAAGTTC 
TATCAGAACT CACCAGTCAT TCACATCTCG GCCAACACCT ATCCGCAACT GTCGTTGCCA 
TCTGTGGTCA CGGTAACGGC GGGTCATCAG TTTGCGGTGA ATCGATGGAA CTGCAACTAC 
ACCGCCTCCG TCCAGAGTCC CAGCTACGCC GAATCGCCCC AATCGCCGGG TTCCAATCAA 
CCACTGACCA TCGATCCGGT TCTGGCTGTT CATGGCACCA ACAACAATAG CAATGCGGCC 
AGCCGACGTC ATTTGGGCGA TAACTTTAGC CAGATGCTAA AGATACGCTC CAACTGCTTT 
GTTACCACAG TGGACAGTCG GTTCCTAATC GCATGCGGAT TCTGGGACAA CAGTTTCCGC 
GTCTTTGCCA CCGAAACGGC GAAAATCGTG CAGATTGTAT TCGGTCACTT TGGTGTGGTA 
ACGTGCATGG CACGTTCCGA GTGCAACATC ACATCAGACT GCTACATCGC CTCCGGATCG 
GCGGACTGCA CGGTGCTTCT GTGGCACTGG AATGCCCGAA CCCAGAGCAT TGTGGGCGAG 
GGCGATGTGC CCACACCACG GGCCACCTTG ACGGGTCACG AACAGGCCGT GACATCGGTG 
GTGATTAGTG CCGAGTTGGG TCTGGTTGTG TCTGGATCAT CAAATGGACC CGTACTTATC 
CATACGACTT TTGGCGACCT GCTGCGCTCA CTGGATCCAC CAGCGGAGTT CCATTCCCCG 
GAACTGATAA CCATGTCCCG CGAGGGTTTC ATTGTCATCA ACTACGATAA GGGCAATGTG 
GCCGCCTACA CCATCAATGG CAAGAAACTG CGCCACGAGA CGCACAACGA CAATCTACAG 
TGCATGCTAC TGTCGCGCGA TGGTGAATAC CTGATGACCG CCGGTGATCG CGGCATCGTG 
GAGGTGTGGC GCACCTTCAA CCTAGCACCA CTTTATGCCT TCCCCGCATG CAACGCGGGC 
ATCCGATCTT TGGCTCTCAC CCACGATCAG AAATACCTTC TGGCCGGACT TTCGACGGGC 
TCGATCATAG TATTCCACAT CGACTTCAAT CGCTGGCACC ACGAGTACCA GCAGCGCTAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138157.2
CDS	1089..11291
Translation	MADIMRPPYSEIKRPDEIVRMTTADNLKFAVLIGLIEVGQVTNREVVNTVLHLLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVSHFQLLPATLGASALSGGSGSGTGSGTGNDASAAAAAAVAAGQQQQLQLQFQILAAEQEARAIDWSDEKLDLNAAFVKIRAVLTARNAVTLAGSSSTGTTAVATAAAAAAAAGAGAGTTAAATSAAAAAAATQNENDAAVGQQQHATHHHATAATGSADDPLGHLPTGNASSSSSSFEQLRRMSSVSSLNSMVGSADTEEVNQLKAVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_001144685.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant O, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144685.2

ORF Insert Sequence:

ATGGCGGACA TCATGCGTCC GCCGTACAGT GAGATCAAAC GGCCGGACGA AATCGTCAGA 
ATGACAACCG CCGACAATCT CAAGTTCGCC GTCCTCATTG GCCTCATCGA GGTTGGCCAG 
GTGACCAACC GGGAGGTGGT CAACACCGTT CTGCATTTGC TGGTCGGCGG TGAATTCGAT 
ATGGAATTGA ATTTTGTGAT ACAGGATGCG CAGAATATCA AACATATGCT GGAGCTGCTG 
GATCATTGTC CGCCCAATTT GCAGGCGGAG ATTTGGAGCG TATTCATCGC CATCCTGCGC 
AAAAGCGTCC GCAATCTGCA GGCCTGCACC GATGTTGGCC TAATCGAGCA TGTTCTCGTC 
CGCCTGCAAC GCTCCGAAAC GGTTGTGGCA GACCTGCTCA TCGAGATGTT GGGCGTGCTG 
GCCAGCTATA GCATAACGGT GAAGGAGCTA AAGCTCCTCT TCGGTACGAT GAAGGCCACC 
AATGGCAAGT GGCCGCGCCA CTCGGCCAAG TTGCTCAACG TCCTCCGGCA GATGCCACAT 
CGCAACGGAC CAGACGTCTT CTTCAGCTTT CCGGGTCGCA AGGGATCGGC CATGGTACTG 
CCGCCACTGG CCAAATGGCC CTATGAGAAC GGATTCACCT TTACCACCTG GTTCCGCCTG 
GATCCCATCA ATTCGGTGAA TATTGAACGT GAGAAGCCCT ACCTCTACTG CTTCAAGACA 
TCGAAGGGCG TGGGCTATAC GGCGCATTTT GTGGGAAATT GCCTTGTCCT CACCTCAATG 
AAGGTCAAGG GCAAAGGCTT TCAGCATTGT GTCAAATACG AATTCCAGCC ACGAAAGTGG 
TATATGATTG CCATAGTGTA TATATACAAT CGTTGGACGA AAAGCGAAAT CAAGTGCCTC 
GTTAATGGAC AGCTGGCCTC TTCAACGGAG ATGGCCTGGT TTGTTTCCAC AAACGATCCC 
TTTGACAAGT GCTACATTGG AGCCACCCCC GAGTTGGACG AGGAGCGCGT GTTCTGTGGC 
CAAATGTCGG CGATCTACCT TTTCAGCGAG GCACTGACCA CGCAGCAGAT CTGTGCGATG 
CATCGCCTGG GTCCCGGCTA CAAGTCCCAG TTCCGTTTCG ACAACGAGTG CTATCTGAAT 
CTGCCGGACA ATCACAAGCG GGTGAGTCAC TTTCAACTTC TGCCAGCGAC CTTGGGGGCG 
TCAGCACTGT CGGGCGGCAG TGGGAGTGGC ACCGGAAGCG GTACCGGCAA CGATGCATCA 
GCTGCAGCGG CAGCGGCTGT TGCCGCCGGT CAGCAACAGC AGTTGCAGCT GCAGTTCCAA 
ATTCTGGCCG CCGAGCAAGA GGCGCGTGCC ATCGATTGGT CCGATGAGAA GCTCGATTTG 
AATGCGGCTT TTGTGAAAAT TCGAGCGGTT CTCACCGCCC GCAATGCAGT GACCTTGGCT 
GGCAGCAGCA GCACTGGCAC CACTGCAGTA GCCACTGCCG CAGCAGCAGC AGCAGCAGCG 
GGAGCGGGAG CAGGAACAAC TGCTGCAGCA ACATCTGCAG CAGCAGCGGC GGCAGCAACA 
CAAAATGAGA ATGATGCAGC AGTGGGACAG CAGCAACATG CAACACATCA CCATGCCACA 
GCAGCAACTG GCAGTGCGGA CGATCCGCTT GGCCATTTGC CCACTGGAAA TGCTTCTTCT 
TCTTCATCTT CTTTTGAGCA ACTACGTCGA ATGTCCAGTG TGAGCAGCTT GAACTCAATG 
GTCGGAAGCG CCGACACTGA GGAGGTCAAC CAGCTGAAAG CTGTGCTGTA CGATGGCAAA 
CTATCGAATG CCATTGTGTT CATGTACAAT CCGGTGGCCA CCGATGGTCA GTTGTGTCTG 
CAGTCATCGC CCAAGGGAAA CGTTTCATAT TTCGTGCACA CGCCGCATGC GCTGATGTTG 
CAGGATGTGA AGGCCGTGGT CACGCACTCG ATACACTGCA CCCTCAATTC GATTGGCGGC 
ATCCAGGTGC TATTCCCGCT TTTCTCGCAG CTGGATATGG CCCACGAGGG TCTGGGCGAC 
ATTAAGCGGG ATCCCACGCT GTGCTCCAAG CTGCTGGGCT TCATCTGTGA ACTGGTGGAA 
ACATCGCAGA CGGTGCAGCA GCATATGATC CAGAACCGGG GATTCCTGGT CATCTCGTTC 
ATGCTGCAGC GCTCCTCCCG CGAACACCTG ACTCTCGAAG TCCTGGGATC ATTCCTGAAT 
CTGACCAAGT ATCTGGTCAC CTGCCTGTCG GCCAACAGTG ATCTGCTGCT CAAACAGCTG 
CTGGATCACG TCCTCTTCAA TCCAGCCCTG TGGATATACA CACCCGCCAA TGTCCAGGCG 
CGACTATACT CCTACTTGGC CACCGAGTTC CTCTCGGACA CGCAGATCTA CAGCAATGTG 
AGGAGGGTCA GCACGGTACT GCAGACAGTG CACACCCTAA AGTACTACTA CTGGGTGGTC 
AATCCACGGG CTAAGAGCGG CATCATTCCC AAGGGACTGG ATGGACCTCG TCCGGCTCAA 
AAGGACATCC TGGCCATCCG GGCATACATC CTGCTGTTCC TGAAGCAGCT CATAATGATC 
GGCAATGGCG TGAAGGAGGA CGAGCTGCAG AGCATACTCA ACTATCTGAC CACCATGCAC 
GAGGACGAGA ACCTGCACGA CGTGCTGCAG ATGCTCATCT CGCTGATGTC GGAGCATCCC 
AGCTCCATGG TACCTGCCTT TGATGTGAAG CACGGCGTGC GCAGCATCTT CAAGTTGCTG 
GCGGCCGAAA GTCAGTTGAT TCGGCTTCAG GCCCTCAAGC TGCTGGGCTT CTTCCTTTCC 
CGCAGCACCC ACAAACGCAA GTACGATGTG ATGTCACCAC ACAATCTGTA CACCCTTTTA 
GCGGAGCGAC TGCTTCTCTA TGAGGAATCA CTGTCCCTGC CCACATACAA TGTTCTCTAT 
GAGATCATGA CGGAGCACAT CTCGCAGCAG ATTCTATACA CACGCCATCC GGAACCAGAG 
AGTCACTACC GTCTGGAGAA TCCAATGATC CTCAAGGTGG TGGCCACCCT GATCCGGCAA 
TCAAAGCAGA CGGAATCGCT GATCGACGTT AAAAAGCTAT TCCTGCAGGA TATGACCCTG 
TTGTGCAATA GCAATCGGGA GAACCGGCGC ACAGTGCTCC AGATGTCCGT GTGGCAGGAG 
TGGCTCATTG CGATGGCGTA CATTCATCCA AAGAGCAGCG AGGAGCAGAA GATAAGCGAC 
ATGGTGTATT CCCTATTCCG CATGCTGCTC CACCATGCCA TTAAGCATGA ATACGGTGGC 
TGGAGAGTTT GGGTAGATAC CCTGGCAATC GTCCACTCCA AGGTGTCTTA CGAGGAGTTT 
AAGCTCCAGT TTGCCCAGAT GTACGAGCAC TACGAGCGCC AGCGAACGGA CAATATCACA 
GATCCTGCCC TGCGTCAGGC TAGACCCATC AGCACGATCA GTGGTTGGGA GCGAGAGGAG 
CTGCATCAGC AGCAGAACGG TGGATCTGCA GCAGCAGTGG CTACCAATCA AACGGCAGCT 
GTTAAAGGTT CTGTTTCCAT TGCCTCACTG GAAGATGTGC CACCGGTGGT TGAGGAAGAG 
GTGGAGGAAC TGGAGCTGGA GGAAGTAGAG ATTCAAGAGG GTCCTATAAC CGAAGAAACC 
GAACAAAAAT CGGTGATAGC CAACATTTCC GACGTCTACA ATGAGCAGCT CAAAACCGAT 
GCCACGTGCA ATGGCAATTT GGAGGATGTC AAAGAGGAGG AGCCTGTACA ACAGCAAATC 
GGAGACCTGG AGAAGCAACC AGAGCCATCT ACTCCTTTGG GAGCACTCAG GGAAACCCTA 
CAACTTGGTG ATGATATGGA TGTGGAAGAA CTGGAGCTGG CTACCGCCAA GGATGCCCTC 
AATGCGGAAC AGCATGTGTC GCGAGTTCTT CAGGCTTCCG AAGCGGCACT TAACGATTGC 
AAAATGGCAG TCGATGATGT CCTGCAGGAG TCATCCTCCG TCCTCAAGGA CGAGGAGATC 
GAACTGGCCG TCAACGAGGT TGTTCAGGGT GTGCTCAATA ACGAGAAGAA AACTCAATCC 
CAGGACAACA AGGATAACAA GGAACAGCCG GGCGAACAGG ATGTAAACGT CAGTCTCCTA 
AACAGCAAGA ATCTGCTCAA CAATAATAAT AATAACAATA ACAACAGTCC GAGTCCAACG 
CCCACAACGG CGACTGCGAC GGCGGAGACG GAAGCGGAAA CAGAAGTCAA TGCCAATGAG 
ATCGTGAGCA GCACAGAGGC GCCCAAGGCG GAAACGGAAA CGAGCGTAGC ACCGGAAGTA 
GAAACACCGG AAACGGCCAA GCCAAGCCCC ATTGTCCCCA GCCCCGTATT AGCAACCAAT 
CAAAAGACTG AAGATGCAGC CAACAAGCTG AACAACAACG AGAAGCTGGC CGAGATCAGC 
GCCAGTCCCG AGCCACCCAT TGTCGTGGAA ACGCCAGAAG CTGATCTTCT TCAGCTTTCC 
GATTCCGAAA CAAAACCGAA TAAGGAAACC GAAGCTGAAG ATTCGGTGGC GCTGGCCGTT 
AGAGATATTG TCGAGCAACT CATCGACAAG GTCATCGATG CCACGGAAGC GGAATCGGCC 
AGCGAAACCA AAACGGAAAC GAATAATAAT GAAATACCCA AGAAGGAGAA GCAGACGTCA 
GAAGAGCCTG AGGATGTGGA AACTGCAGAG ACTTTGGCCG CTGCCGCCAA GGAAATTGTC 
CAAGAGGTTG TGGAAGCAGC TCTCGTAATG GTCCAAGAGG AGAGCACTCA AGAGAAGCCA 
GAAAAGGGGG CGAACTCCGA AGAGGAGAAA AACGAAATTG GCAAGGAAGA GATTCTTCTG 
CAGCTGGAGG AAAAACCAGC GTCTACTGAA GTGCAGGAAA CCAAGATCGA AGGAGATCTA 
AAGAAACCAG AAGATCCAAA AGGTCACAGC TCTGTAGAAC CAAAGACCCC GAACCTTGAG 
GAACCGAAAC CCCAGGAAAC TGAACAACAA AAGTCGCAGG AGGTCGCCGA AGAACTACCT 
CAAAAACCAG AGGAGCAGGT GGTGGCCATT GTCAACCAAG TCCTGGACAC TCTGGTAGAC 
GATACGGTCA AGGCAGTGGC AGCCGAGCAA ACCACTCAAA CATCGCCAGC CCCCGAAGAG 
CAATCGCCAC AAATACTGGC AATGGAATCA CCAGCAACGT CGGTGCGGGT CAAACCCACT 
GAGGTGGATT CCACCACCCA GACTACGCCG AAGAACGAGG CTGGATCCAG TCTATTGGTT 
GAGCAGGTGC AGCAAGTCCT CCAGGAGGAT GACGCCCAGC AATCAGCCGG CATGACCATC 
GAAGATGAGG ATTACTCTAA TCAGCAGGCA GCTGCGGCAG TTGAAAATGC CAACAGCAGC 
CAATTGGATG CCAATCATTA TGGTCCCGGT AATCCAGAAT CCAAACAACA GCAACAGCGC 
TCCAAATCGG GATCGACCCG ACCTATGTTC AGTCCGGGAC CTACACGTCC GCCCTTCCGA 
ATACCGGAAT TCAAGTGGTC CTACATCCAT CAGCGATTGC TCAGCGATGT CCTCTTTTCG 
CTGGAAACGG ACATTCAGGT GTGGCGCAGT CATTCGACCA AAAGCGTCCT GGACTTTGTC 
AACTCCAGCG AGAATGCAAT TTTTGTGGTG AACACGGTGC ATTTAATATC GCAGCTAGCG 
GATAATCTGA TTATTGCCTG CGGAGGATTG CTGCCTCTTT TGGCCAGCGC CACGTCACCC 
AATTCCGAAT TGGATGTGCT GGAGCCAACG CAAGGCATGC CTTTGGAGGT GGCCGTATCT 
TTCCTGCAGC GTCTGGTCAA CATGGCGGAT GTCCTGATCT TTGCCACATC CCTAAACTTC 
GGCGAGTTGG AGGCGGAGAA GAACATGTCC AGTGGCGGCA TACTGCGTCA GTGCCTGCGA 
TTGGTTTGCA CCTGTGCGGT AAGGAATTGC TTGGAGTGCA AGGAGCGAAC GCGTTACAAT 
GTGGGCGCCT TGGCGAGAGA TGTCCCGGGT GCAGCCCATT TGCAGGCCCT CATCCGCGGA 
GCTCAAGCAT CGCCCAAGAA CATTGTCGAG TCAATAACCG GCCAATTATC GCCTGTCAAG 
GATCCCGAGA AGCTGCTCCA GGACATGGAC GTCAATCGCC TGCGCGCAGT CATCTATCGC 
GATGTGGAGG AGACTAAGCA GGCGCAGTTC CTCTCGCTGG CGATAGTGTA CTTCATATCG 
GTGCTGATGG TGTCCAAGTA TCGTGATATC CTGGAGCCGC CGGCAGAGCC GCAAATCCAG 
CGTCAATCGC CAGTGCTGCA ACGCACGGCA GGCGGCGGTG GTCGTCAAAT CCAGGATAGT 
GACTACGAAA TAATTGTCGT CGATGAGAAC AATCCATCGG TTTTGGCGGA TAATGATTCA 
CATTCCAGTG GACCACCATC CATAAAGAGC GTTGACTCGG ATGTGGGCTC CCTGAACATG 
AACTCCACGG AAAACGAAGT GCCCGAGGTG GAGTCATCCA GCGAGATTCT AATCGATGAC 
CACAAACCGA GTCACTCGAA CGACGAAAGC TGGACGGATG TGAATCTCAA TGAGGATGCT 
GCCGTTCAGG CGGCAAGTGC CGGAATAGTT GTAGGTTTGG TGGATAACAG AGGTAATGTT 
ATATCCGACA AACACGATCC GTCCCATCAC AACCAACAAC AGCAGCAGCA GGCGGGAATT 
ATTGGTCAGC AGCAGCAACA TGGATCACTT GGTCATTCGG AGCGGGGTGA CAAACCCGAT 
TCGGAGATTT CGGTGGTGCG AGTGCCTGAT GGTTATGGTG GTGCTGGTTC CGGTGGTGGC 
GTGAATTCCG GACAAGGTCA GGGCGTTCCA TCCAATCAGC GTCCTCGTCC CGAGGAGTTG 
CCCATGAAGG CGCCCGCCTT GGTGGCCCAA CTGCCGCTGA CCACACCTTC GCGAGAGGCC 
AGTCTCACCC AGAAGCTGGA AATCGCATTG GGGCCAGTGT GTCCGCTGCT CCGTGAAATC 
ATGGTGGACT TTGCGCCGTT CCTTTCGAAA ACCCTAGTCG GCTCCCATGG CCAGGAATTG 
CTGATGGAGG GCAAGGGTCT GACGACCTTT AAGAACTCGC ATTCCGTCGT AGAGCTGGTC 
ATGTTGCTCT GCTCCCAGGA GTGGCAAAAT AGCCTACAAA AGCACGCAGG ATTAGCCTTT 
ATTGAGCTAA TCAATGAGGG TCGACTGCTT TCGCACGCCA TGAAGGATCA CATTGTTCGG 
GTGGCCAATG AGGCGGAGTT CATCCTCAAT AGAATGCGAG CGGATGATGT CCTCAAGCAT 
GCCGACTTTG AATCGCAATG TGCACAGACA CTTTTGGAGC GCAGGGAGGA GGAGCGAATG 
TGCGATCACT TGATAACCGC CGCACGTCGC AGAGACAATG TGATTGCCAG CCGACTGCTG 
GAGAAGGTAC GAAACATCAT GTGTAATCGC CACGGAGCCT GGGGTGATTC CAGCTCCACA 
TCCAGTGGTG GCGCCATCGT TGGAGCGGTG CAAAAGAGTC CCTACTGGAA GCTGGATGCC 
TGGGAGGATG ATGCCAGACG TCGGAAACGG ATGGTGCAAA ATCCGCGCGG ATCCTCGCAT 
CCACAGGCCA CACTGAAGGC GGCTTTGGAA AATGGCGGAC CTGAAGATGC CATCCTGCAG 
ACTCGCGACG AGTTCCACAC CCAAATAGCC GTCTCGCGGA CTCATCCATC CGGTCAGCAC 
AATGGTGAGC TACTGGACGA CGCCGAGCTC TTGATTGAGG ATCGGGAGTT GGATCTGGAT 
CTCACGGGTC CCGTCAACAT TAGCACCAAG GCGAGATTGA TAGCCCCCGG TCTGGTGGCA 
CCCGGCACCG TCTCAATAAC CAGCACTGAA ATGTTCTTTG AGGTGGATGA AGAGCATCCC 
GAGTTCCAGA AGATCGATGG CGAAGTTCTT AAGTACTGTG ACCATTTGCA CGGCAAATGG 
TACTTCTCCG AAGTGAGGGC CATCTTCTCG AGGCGTTATC TCCTGCAAAA TGTGGCCCTC 
GAGATCTTTT TGGCCAGCCG TACGTCCATA TTGTTTGCCT TTCCCGATCA GCATACGGTC 
AAGAAGGTAA TCAAGGCCCT GCCCCGTGTG GGAGTGGGCA TCAAGTATGG AATACCGCAG 
ACGAGGAGGG CCTCAATGAT GTCGCCCCGT CAACTGATGC GCAATTCGAA CATGACCCAA 
AAGTGGCAGC GTCGCGAGAT TAGCAACTTT GAGTATCTTA TGTTCCTGAA CACGATCGCC 
GGCAGGACGT ACAACGATCT AAATCAGTAT CCCATCTTTC CGTGGGTGCT GACCAACTAT 
GAGTCCAAGG ATTTGGATCT CAGCCTGCCG TCAAACTACA GGGATCTGTC GAAACCGATT 
GGCGCCCTGA ATCCATCGCG CAGAGCCTAT TTCGAGGAGC GTTACGAGAG CTGGGACAGT 
GACACCATAC CGCCCTTCCA CTATGGTACG CATTATTCCA CGGCAGCATT TACGCTGAAC 
TGGTTGGTGC GCGTGGAACC GTTTACCACC ATGTTCCTGG CCCTGCAGGG CGGCAAATTT 
GATTATCCGG ACAGGCTGTT CAGCTCGGTG TCGCTGTCCT GGAAGAATTG CCAACGTGAT 
ACATCGGATG TGAAGGAACT GATACCAGAA TGGTATTTCC TGCCCGAAAT GTTTTACAAT 
TCATCAGGCT ATCGACTGGG TCATCGCGAG GATGGTGCCC TAGTGGATGA CATTGAACTA 
CCACCGTGGG CCAAGAGCCC CGAAGAATTC GTCCGCATCA ATCGCATGGC ACTGGAGTCG 
GAGTTCGTGT CCTGCCAGCT GCACCAGTGG ATCGATTTGA TCTTTGGCTA CAAGCAACGC 
GGTCCCGAGG CCATTAGGGC AACCAACGTG TTCTACTATT TGACATATGA GGGCAGCGTT 
GACTTGGATG GCGTTTTGGA TCCGGTGATG CGCGAAGCCG TCGAGAATCA AATCCGCAAC 
TTTGGCCAAA CACCTAGTCA ACTGCTAATG GAACCGCATC CGCCGCGCAG CTCTGCGATG 
CATCTATCGC CTATGATGTT CAGTGCGATG CCGGAGGATC TCTGCCAGAT GCTCAAGTTC 
TATCAGAACT CACCAGTCAT TCACATCTCG GCCAACACCT ATCCGCAACT GTCGTTGCCA 
TCTGTGGTCA CGGTAACGGC GGGTCATCAG TTTGCGGTGA ATCGATGGAA CTGCAACTAC 
ACCGCCTCCG TCCAGAGTCC CAGCTACGCC GAATCGCCCC AATCGCCGGG TTCCAATCAA 
CCACTGACCA TCGATCCGGT TCTGGCTGTT CATGGCACCA ACAACAATAG CAATGCGGCC 
AGCCGACGTC ATTTGGGCGA TAACTTTAGC CAGATGCTAA AGATACGCTC CAACTGCTTT 
GTTACCACAG TGGACAGTCG GTTCCTAATC GCATGCGGAT TCTGGGACAA CAGTTTCCGC 
GTCTTTGCCA CCGAAACGGC GAAAATCGTG CAGATTGTAT TCGGTCACTT TGGTGTGGTA 
ACGTGCATGG CACGTTCCGA GTGCAACATC ACATCAGACT GCTACATCGC CTCCGGATCG 
GCGGACTGCA CGGTGCTTCT GTGGCACTGG AATGCCCGAA CCCAGAGCAT TGTGGGCGAG 
GGCGATGTGC CCACACCACG GGCCACCTTG ACGGGTCACG AACAGGCCGT GACATCGGTG 
GTGATTAGTG CCGAGTTGGG TCTGGTTGTG TCTGGATCAT CAAATGGACC CGTACTTATC 
CATACGACTT TTGGCGACCT GCTGCGCTCA CTGGATCCAC CAGCGGAGTT CCATTCCCCG 
GAACTGATAA CCATGTCCCG CGAGGGTTTC ATTGTCATCA ACTACGATAA GGGCAATGTG 
GCCGCCTACA CCATCAATGG CAAGAAACTG CGCCACGAGA CGCACAACGA CAATCTACAG 
TGCATGCTAC TGTCGCGCGA TGGTGAATAC CTGATGACCG CCGGTGATCG CGGCATCGTG 
GAGGTGTGGC GCACCTTCAA CCTAGCACCA CTTTATGCCT TCCCCGCATG CAACGCGGGC 
ATCCGATCTT TGGCTCTCAC CCACGATCAG AAATACCTTC TGGCCGGACT TTCGACGGGC 
TCGATCATAG TATTCCACAT CGACTTCAAT CGCTGGCACC ACGAGTACCA GCAGCGCTAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rg ( NM_167028.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726978.2 Drosophila melanogaster rugose (rg), transcript variant R, mRNA.

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CloneID	OFa22220
Clone ID Related Accession (Same CDS sequence)	NM_167028.3
Accession Version	NM_167028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rugose, isoform R
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167028.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACTCGC TGGAGCGTCT GATGCGAGCG GCCCCATTGC CACGAATGCT TACCAGTGGC 
GTTGTGGCCA CAGCCGCTGC CGCCGCATCC GCCGCCGCTG GCAAGGGCAT GGTCAGTGTG 
GGCGGTGGTG CAACAGCCAT AGCCGCCGGC GGTGGCCAAC AGAGGGCGCT GGTCCATGCC 
TCGCTGGCGG CGGCGACAGT GGGCCGCAAG GGACGCCACC TAACGGGCAC CTTCTGCCTA 
ACCGGCGACA CCATGGAGGG CATCATACAG TGTTTGGTAT TTCTCAAAGC ATTCTCGCTG 
GTCGGCGGTG AATTCGATAT GGAATTGAAT TTTGTGATAC AGGATGCGCA GAATATCAAA 
CATATGCTGG AGCTGCTGGA TCATTGTCCG CCCAATTTGC AGGCGGAGAT TTGGAGCGTA 
TTCATCGCCA TCCTGCGCAA AAGCGTCCGC AATCTGCAGG CCTGCACCGA TGTTGGCCTA 
ATCGAGCATG TTCTCGTCCG CCTGCAACGC TCCGAAACGG TTGTGGCAGA CCTGCTCATC 
GAGATGTTGG GCGTGCTGGC CAGCTATAGC ATAACGGTGA AGGAGCTAAA GCTCCTCTTC 
GGTACGATGA AGGCCACCAA TGGCAAGTGG CCGCGCCACT CGGCCAAGTT GCTCAACGTC 
CTCCGGCAGA TGCCACATCG CAACGGACCA GACGTCTTCT TCAGCTTTCC GGGTCGCAAG 
GGATCGGCCA TGGTACTGCC GCCACTGGCC AAATGGCCCT ATGAGAACGG ATTCACCTTT 
ACCACCTGGT TCCGCCTGGA TCCCATCAAT TCGGTGAATA TTGAACGTGA GAAGCCCTAC 
CTCTACTGCT TCAAGACATC GAAGGGCGTG GGCTATACGG CGCATTTTGT GGGAAATTGC 
CTTGTCCTCA CCTCAATGAA GGTCAAGGGC AAAGGCTTTC AGCATTGTGT CAAATACGAA 
TTCCAGCCAC GAAAGTGGTA TATGATTGCC ATAGTGTATA TATACAATCG TTGGACGAAA 
AGCGAAATCA AGTGCCTCGT TAATGGACAG CTGGCCTCTT CAACGGAGAT GGCCTGGTTT 
GTTTCCACAA ACGATCCCTT TGACAAGTGC TACATTGGAG CCACCCCCGA GTTGGACGAG 
GAGCGCGTGT TCTGTGGCCA AATGTCGGCG ATCTACCTTT TCAGCGAGGC ACTGACCACG 
CAGCAGATCT GTGCGATGCA TCGCCTGGGT CCCGGCTACA AGTCCCAGTT CCGTTTCGAC 
AACGAGTGCT ATCTGAATCT GCCGGACAAT CACAAGCGGG TGCTGTACGA TGGCAAACTA 
TCGAATGCCA TTGTGTTCAT GTACAATCCG GTGGCCACCG ATGGTCAGTT GTGTCTGCAG 
TCATCGCCCA AGGGAAACGT TTCATATTTC GTGCACACGC CGCATGCGCT GATGTTGCAG 
GATGTGAAGG CCGTGGTCAC GCACTCGATA CACTGCACCC TCAATTCGAT TGGCGGCATC 
CAGGTGCTAT TCCCGCTTTT CTCGCAGCTG GATATGGCCC ACGAGGGTCT GGGCGACATT 
AAGCGGGATC CCACGCTGTG CTCCAAGCTG CTGGGCTTCA TCTGTGAACT GGTGGAAACA 
TCGCAGACGG TGCAGCAGCA TATGATCCAG AACCGGGGAT TCCTGGTCAT CTCGTTCATG 
CTGCAGCGCT CCTCCCGCGA ACACCTGACT CTCGAAGTCC TGGGATCATT CCTGAATCTG 
ACCAAGTATC TGGTCACCTG CCTGTCGGCC AACAGTGATC TGCTGCTCAA ACAGCTGCTG 
GATCACGTCC TCTTCAATCC AGCCCTGTGG ATATACACAC CCGCCAATGT CCAGGCGCGA 
CTATACTCCT ACTTGGCCAC CGAGTTCCTC TCGGACACGC AGATCTACAG CAATGTGAGG 
AGGGTCAGCA CGGTACTGCA GACAGTGCAC ACCCTAAAGT ACTACTACTG GGTGGTCAAT 
CCACGGGCTA AGAGCGGCAT CATTCCCAAG GGACTGGATG GACCTCGTCC GGCTCAAAAG 
GACATCCTGG CCATCCGGGC ATACATCCTG CTGTTCCTGA AGCAGCTCAT AATGATCGGC 
AATGGCGTGA AGGAGGACGA GCTGCAGAGC ATACTCAACT ATCTGACCAC CATGCACGAG 
GACGAGAACC TGCACGACGT GCTGCAGATG CTCATCTCGC TGATGTCGGA GCATCCCAGC 
TCCATGGTAC CTGCCTTTGA TGTGAAGCAC GGCGTGCGCA GCATCTTCAA GTTGCTGGCG 
GCCGAAAGTC AGTTGATTCG GCTTCAGGCC CTCAAGCTGC TGGGCTTCTT CCTTTCCCGC 
AGCACCCACA AACGCAAGTA CGATGTGATG TCACCACACA ATCTGTACAC CCTTTTAGCG 
GAGCGACTGC TTCTCTATGA GGAATCACTG TCCCTGCCCA CATACAATGT TCTCTATGAG 
ATCATGACGG AGCACATCTC GCAGCAGATT CTATACACAC GCCATCCGGA ACCAGAGAGT 
CACTACCGTC TGGAGAATCC AATGATCCTC AAGGTGGTGG CCACCCTGAT CCGGCAATCA 
AAGCAGACGG AATCGCTGAT CGACGTTAAA AAGCTATTCC TGCAGGATAT GACCCTGTTG 
TGCAATAGCA ATCGGGAGAA CCGGCGCACA GTGCTCCAGA TGTCCGTGTG GCAGGAGTGG 
CTCATTGCGA TGGCGTACAT TCATCCAAAG AGCAGCGAGG AGCAGAAGAT AAGCGACATG 
GTGTATTCCC TATTCCGCAT GCTGCTCCAC CATGCCATTA AGCATGAATA CGGTGGCTGG 
AGAGTTTGGG TAGATACCCT GGCAATCGTC CACTCCAAGG TGTCTTACGA GGAGTTTAAG 
CTCCAGTTTG CCCAGATGTA CGAGCACTAC GAGCGCCAGC GAACGGACAA TATCACAGAT 
CCTGCCCTGC GTCAGGCTAG ACCCATCAGC ACGATCAGTG GTTGGGAGCG AGAGGAGCTG 
CATCAGCAGC AGAACGGTGG ATCTGCAGCA GCAGTGGCTA CCAATCAAAC GGCAGCTGTT 
AAAGGTTCTG TTTCCATTGC CTCACTGGAA GATGTGCCAC CGGTGGTTGA GGAAGAGGTG 
GAGGAACTGG AGCTGGAGGA AGTAGAGATT CAAGAGGGTC CTATAACCGA AGAAACCGAA 
CAAAAATCGG TGATAGCCAA CATTTCCGAC GTCTACAATG AGCAGCTCAA AACCGATGCC 
ACGTGCAATG GCAATTTGGA GGATGTCAAA GAGGAGGAGC CTGTACAACA GCAAATCGGA 
GACCTGGAGA AGCAACCAGA GCCATCTACT CCTTTGGGAG CACTCAGGGA AACCCTACAA 
CTTGGTGATG ATATGGATGT GGAAGAACTG GAGCTGGCTA CCGCCAAGGA TGCCCTCAAT 
GCGGAACAGC ATGTGTCGCG AGTTCTTCAG GCTTCCGAAG CGGCACTTAA CGATTGCAAA 
ATGGCAGTCG ATGATGTCCT GCAGGAGTCA TCCTCCGTCC TCAAGGACGA GGAGATCGAA 
CTGGCCGTCA ACGAGGTTGT TCAGGGTGTG CTCAATAACG AGAAGAAAAC TCAATCCCAG 
GACAACAAGG ATAACAAGGA ACAGCCGGGC GAACAGGATG TAAACGTCAG TCTCCTAAAC 
AGCAAGAATC TGCTCAACAA TAATAATAAT AACAATAACA ACAGTCCGAG TCCAACGCCC 
ACAACGGCGA CTGCGACGGC GGAGACGGAA GCGGAAACAG AAGTCAATGC CAATGAGATC 
GTGAGCAGCA CAGAGGCGCC CAAGGCGGAA ACGGAAACGA GCGTAGCACC GGAAGTAGAA 
ACACCGGAAA CGGCCAAGCC AAGCCCCATT GTCCCCAGCC CCGTATTAGC AACCAATCAA 
AAGACTGAAG ATGCAGCCAA CAAGCTGAAC AACAACGAGA AGCTGGCCGA GATCAGCGCC 
AGTCCCGAGC CACCCATTGT CGTGGAAACG CCAGAAGCTG ATCTTCTTCA GCTTTCCGAT 
TCCGAAACAA AACCGAATAA GGAAACCGAA GCTGAAGATT CGGTGGCGCT GGCCGTTAGA 
GATATTGTCG AGCAACTCAT CGACAAGGTC ATCGATGCCA CGGAAGCGGA ATCGGCCAGC 
GAAACCAAAA CGGAAACGAA TAATAATGAA ATACCCAAGA AGGAGAAGCA GACGTCAGAA 
GAGCCTGAGG ATGTGGAAAC TGCAGAGACT TTGGCCGCTG CCGCCAAGGA AATTGTCCAA 
GAGGTTGTGG AAGCAGCTCT CGTAATGGTC CAAGAGGAGA GCACTCAAGA GAAGCCAGAA 
AAGGGGGCGA ACTCCGAAGA GGAGAAAAAC GAAATTGGCA AGGAAGAGAT TCTTCTGCAG 
CTGGAGGAAA AACCAGCGTC TACTGAAGTG CAGGAAACCA AGATCGAAGG AGATCTAAAG 
AAACCAGAAG ATCCAAAAGG TCACAGCTCT GTAGAACCAA AGACCCCGAA CCTTGAGGAA 
CCGAAACCCC AGGAAACTGA ACAACAAAAG TCGCAGGAGG TCGCCGAAGA ACTACCTCAA 
AAACCAGAGG AGCAGGTGGT GGCCATTGTC AACCAAGTCC TGGACACTCT GGTAGACGAT 
ACGGTCAAGG CAGTGGCAGC CGAGCAAACC ACTCAAACAT CGCCAGCCCC CGAAGAGCAA 
TCGCCACAAA TACTGGCAAT GGAATCACCA GCAACGTCGG TGCGGGTCAA ACCCACTGAG 
GTGGATTCCA CCACCCAGAC TACGCCGAAG AACGAGGCTG GATCCAGTCT ATTGGTTGAG 
CAGGTGCAGC AAGTCCTCCA GGAGGATGAC GCCCAGCAAT CAGCCGGCAT GACCATCGAA 
GATGAGGATT ACTCTAATCA GCAGGCAGCT GCGGCAGTTG AAAATGCCAA CAGCAGCCAA 
TTGGATGCCA ATCATTATGG TCCCGGTAAT CCAGAATCCA AACAACAGCA ACAGCGCTCC 
AAATCGGGAT CGACCCGACC TATGTTCAGT CCGGGACCTA CACGTCCGCC CTTCCGAATA 
CCGGAATTCA AGTGGTCCTA CATCCATCAG CGATTGCTCA GCGATGTCCT CTTTTCGCTG 
GAAACGGACA TTCAGGTGTG GCGCAGTCAT TCGACCAAAA GCGTCCTGGA CTTTGTCAAC 
TCCAGCGAGA ATGCAATTTT TGTGGTGAAC ACGGTGCATT TAATATCGCA GCTAGCGGAT 
AATCTGATTA TTGCCTGCGG AGGATTGCTG CCTCTTTTGG CCAGCGCCAC GTCACCCAAT 
TCCGAATTGG ATGTGCTGGA GCCAACGCAA GGCATGCCTT TGGAGGTGGC CGTATCTTTC 
CTGCAGCGTC TGGTCAACAT GGCGGATGTC CTGATCTTTG CCACATCCCT AAACTTCGGC 
GAGTTGGAGG CGGAGAAGAA CATGTCCAGT GGCGGCATAC TGCGTCAGTG CCTGCGATTG 
GTTTGCACCT GTGCGGTAAG GAATTGCTTG GAGTGCAAGG AGCGAACGCG TTACAATGTG 
GGCGCCTTGG CGAGAGATGT CCCGGGTGCA GCCCATTTGC AGGCCCTCAT CCGCGGAGCT 
CAAGCATCGC CCAAGAACAT TGTCGAGTCA ATAACCGGCC AATTATCGCC TGTCAAGGAT 
CCCGAGAAGC TGCTCCAGGA CATGGACGTC AATCGCCTGC GCGCAGTCAT CTATCGCGAT 
GTGGAGGAGA CTAAGCAGGC GCAGTTCCTC TCGCTGGCGA TAGTGTACTT CATATCGGTG 
CTGATGGTGT CCAAGTATCG TGATATCCTG GAGCCGCCGG CAGAGCCGCA AATCCAGCGT 
CAATCGCCAG TGCTGCAACG CACGGCAGGC GGCGGTGGTC GTCAAATCCA GGATAGTGAC 
TACGAAATAA TTGTCGTCGA TGAGAACAAT CCATCGGTTT TGGCGGATAA TGATTCACAT 
TCCAGTGGAC CACCATCCAT AAAGAGCGTT GACTCGGATG TGGGCTCCCT GAACATGAAC 
TCCACGGAAA ACGAAGTGCC CGAGGTGGAG TCATCCAGCG AGATTCTAAT CGATGACCAC 
AAACCGAGTC ACTCGAACGA CGAAAGCTGG ACGGATGTGA ATCTCAATGA GGATGCTGCC 
GTTCAGGCGG CAAGTGCCGG AATAGTTGTA GGTTTGGTGG ATAACAGAGG TAATGTTATA 
TCCGACAAAC ACGATCCGTC CCATCACAAC CAACAACAGC AGCAGCAGGC GGGAATTATT 
GGTCAGCAGC AGCAACATGG ATCACTTGGT CATTCGGAGC GGGGTGACAA ACCCGATTCG 
GAGATTTCGG TGGTGCGAGT GCCTGATGGT TATGGTGGTG CTGGTTCCGG TGGTGGCGTG 
AATTCCGGAC AAGGTCAGGG CGTTCCATCC AATCAGCGTC CTCGTCCCGA GGAGTTGCCC 
ATGAAGGCGC CCGCCTTGGT GGCCCAACTG CCGCTGACCA CACCTTCGCG AGAGGCCAGT 
CTCACCCAGA AGCTGGAAAT CGCATTGGGG CCAGTGTGTC CGCTGCTCCG TGAAATCATG 
GTGGACTTTG CGCCGTTCCT TTCGAAAACC CTAGTCGGCT CCCATGGCCA GGAATTGCTG 
ATGGAGGGCA AGGGTCTGAC GACCTTTAAG AACTCGCATT CCGTCGTAGA GCTGGTCATG 
TTGCTCTGCT CCCAGGAGTG GCAAAATAGC CTACAAAAGC ACGCAGGATT AGCCTTTATT 
GAGCTAATCA ATGAGGGTCG ACTGCTTTCG CACGCCATGA AGGATCACAT TGTTCGGGTG 
GCCAATGAGG CGGAGTTCAT CCTCAATAGA ATGCGAGCGG ATGATGTCCT CAAGCATGCC 
GACTTTGAAT CGCAATGTGC ACAGACACTT TTGGAGCGCA GGGAGGAGGA GCGAATGTGC 
GATCACTTGA TAACCGCCGC ACGTCGCAGA GACAATGTGA TTGCCAGCCG ACTGCTGGAG 
AAGGTACGAA ACATCATGTG TAATCGCCAC GGAGCCTGGG GTGATTCCAG CTCCACATCC 
AGTGGTGGCG CCATCGTTGG AGCGGTGCAA AAGAGTCCCT ACTGGAAGCT GGATGCCTGG 
GAGGATGATG CCAGACGTCG GAAACGGATG GTGCAAAATC CGCGCGGATC CTCGCATCCA 
CAGGCCACAC TGAAGGCGGC TTTGGAAAAT GGCGGACCTG AAGATGCCAT CCTGCAGACT 
CGCGACGAGT TCCACACCCA AATAGCCGTC TCGCGGACTC ATCCATCCGG TCAGCACAAT 
GGTGAGCTAC TGGACGACGC CGAGCTCTTG ATTGAGGATC GGGAGTTGGA TCTGGATCTC 
ACGGGTCCCG TCAACATTAG CACCAAGGCG AGATTGATAG CCCCCGGTCT GGTGGCACCC 
GGCACCGTCT CAATAACCAG CACTGAAATG TTCTTTGAGG TGGATGAAGA GCATCCCGAG 
TTCCAGAAGA TCGATGGCGA AGTTCTTAAG TACTGTGACC ATTTGCACGG CAAATGGTAC 
TTCTCCGAAG TGAGGGCCAT CTTCTCGAGG CGTTATCTCC TGCAAAATGT GGCCCTCGAG 
ATCTTTTTGG CCAGCCGTAC GTCCATATTG TTTGCCTTTC CCGATCAGCA TACGGTCAAG 
AAGGTAATCA AGGCCCTGCC CCGTGTGGGA GTGGGCATCA AGTATGGAAT ACCGCAGACG 
AGGAGGGCCT CAATGATGTC GCCCCGTCAA CTGATGCGCA ATTCGAACAT GACCCAAAAG 
TGGCAGCGTC GCGAGATTAG CAACTTTGAG TATCTTATGT TCCTGAACAC GATCGCCGGC 
AGGACGTACA ACGATCTAAA TCAGTATCCC ATCTTTCCGT GGGTGCTGAC CAACTATGAG 
TCCAAGGATT TGGATCTCAG CCTGCCGTCA AACTACAGGG ATCTGTCGAA ACCGATTGGC 
GCCCTGAATC CATCGCGCAG AGCCTATTTC GAGGAGCGTT ACGAGAGCTG GGACAGTGAC 
ACCATACCGC CCTTCCACTA TGGTACGCAT TATTCCACGG CAGCATTTAC GCTGAACTGG 
TTGGTGCGCG TGGAACCGTT TACCACCATG TTCCTGGCCC TGCAGGGCGG CAAATTTGAT 
TATCCGGACA GGCTGTTCAG CTCGGTGTCG CTGTCCTGGA AGAATTGCCA ACGTGATACA 
TCGGATGTGA AGGAACTGAT ACCAGAATGG TATTTCCTGC CCGAAATGTT TTACAATTCA 
TCAGGCTATC GACTGGGTCA TCGCGAGGAT GGTGCCCTAG TGGATGACAT TGAACTACCA 
CCGTGGGCCA AGAGCCCCGA AGAATTCGTC CGCATCAATC GCATGGCACT GGAGTCGGAG 
TTCGTGTCCT GCCAGCTGCA CCAGTGGATC GATTTGATCT TTGGCTACAA GCAACGCGGT 
CCCGAGGCCA TTAGGGCAAC CAACGTGTTC TACTATTTGA CATATGAGGG CAGCGTTGAC 
TTGGATGGCG TTTTGGATCC GGTGATGCGC GAAGCCGTCG AGAATCAAAT CCGCAACTTT 
GGCCAAACAC CTAGTCAACT GCTAATGGAA CCGCATCCGC CGCGCAGCTC TGCGATGCAT 
CTATCGCCTA TGATGTTCAG TGCGATGCCG GAGGATCTCT GCCAGATGCT CAAGTTCTAT 
CAGAACTCAC CAGTCATTCA CATCTCGGCC AACACCTATC CGCAACTGTC GTTGCCATCT 
GTGGTCACGG TAACGGCGGG TCATCAGTTT GCGGTGAATC GATGGAACTG CAACTACACC 
GCCTCCGTCC AGAGTCCCAG CTACGCCGAA TCGCCCCAAT CGCCGGGTTC CAATCAACCA 
CTGACCATCG ATCCGGTTCT GGCTGTTCAT GGCACCAACA ACAATAGCAA TGCGGCCAGC 
CGACGTCATT TGGGCGATAA CTTTAGCCAG ATGCTAAAGA TACGCTCCAA CTGCTTTGTT 
ACCACAGTGG ACAGTCGGTT CCTAATCGCA TGCGGATTCT GGGACAACAG TTTCCGCGTC 
TTTGCCACCG AAACGGCGAA AATCGTGCAG ATTGTATTCG GTCACTTTGG TGTGGTAACG 
TGCATGGCAC GTTCCGAGTG CAACATCACA TCAGACTGCT ACATCGCCTC CGGATCGGCG 
GACTGCACGG TGCTTCTGTG GCACTGGAAT GCCCGAACCC AGAGCATTGT GGGCGAGGGC 
GATGTGCCCA CACCACGGGC CACCTTGACG GGTCACGAAC AGGCCGTGAC ATCGGTGGTG 
ATTAGTGCCG AGTTGGGTCT GGTTGTGTCT GGATCATCAA ATGGACCCGT ACTTATCCAT 
ACGACTTTTG GCGACCTGCT GCGCTCACTG GATCCACCAG CGGAGTTCCA TTCCCCGGAA 
CTGATAACCA TGTCCCGCGA GGGTTTCATT GTCATCAACT ACGATAAGGG CAATGTGGCC 
GCCTACACCA TCAATGGCAA GAAACTGCGC CACGAGACGC ACAACGACAA TCTACAGTGC 
ATGCTACTGT CGCGCGATGG TGAATACCTG ATGACCGCCG GTGATCGCGG CATCGTGGAG 
GTGTGGCGCA CCTTCAACCT AGCACCACTT TATGCCTTCC CCGCATGCAA CGCGGGCATC 
CGATCTTTGG CTCTCACCCA CGATCAGAAA TACCTTCTGG CCGGACTTTC GACGGGCTCG 
ATCATAGTAT TCCACATCGA CTTCAATCGC TGGCACCACG AGTACCAGCA GCGCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726978.2
CDS	2312..12031
Translation	MDSLERLMRAAPLPRMLTSGVVATAAAAASAAAGKGMVSVGGGATAIAAGGGQQRALVHASLAAATVGRKGRHLTGTFCLTGDTMEGIIQCLVFLKAFSLVGGEFDMELNFVIQDAQNIKHMLELLDHCPPNLQAEIWSVFIAILRKSVRNLQACTDVGLIEHVLVRLQRSETVVADLLIEMLGVLASYSITVKELKLLFGTMKATNGKWPRHSAKLLNVLRQMPHRNGPDVFFSFPGRKGSAMVLPPLAKWPYENGFTFTTWFRLDPINSVNIEREKPYLYCFKTSKGVGYTAHFVGNCLVLTSMKVKGKGFQHCVKYEFQPRKWYMIAIVYIYNRWTKSEIKCLVNGQLASSTEMAWFVSTNDPFDKCYIGATPELDEERVFCGQMSAIYLFSEALTTQQICAMHRLGPGYKSQFRFDNECYLNLPDNHKRVLYDGKLSNAIVFMYNPVATDGQLCLQSSPKGNVSYFVHTPHALMLQDVKAVVTHSIHCTLNSIGGIQVLFPLFSQLDMAHEGLGDIKRDPTLCSKLLGFICELVETSQTVQQHMIQNRGFLVISFMLQRSSREHLTLEVLGSFLNLTKYLVTCLSANSDLLLKQLLDHVLFNPALWIYTPANVQARLYSYLATEFLSDTQIYSNVRRVSTVLQTVHTLKYYYWVVNPRAKSGIIPKGLDGPRPAQKDILAIRAYILLFLKQLIMIGNGVKEDELQSILNYLTTMHEDENLHDVLQMLISLMSEHPSSMVPAFDVKHGVRSIFKLLAAESQLIRLQALKLLGFFLSRSTHKRKYDVMSPHNLYTLLAERLLLYEESLSLPTYNVLYEIMTEHISQQILYTRHPEPESHYRLENPMILKVVATLIRQSKQTESLIDVKKLFLQDMTLLCNSNRENRRTVLQMSVWQEWLIAMAYIHPKSSEEQKISDMVYSLFRMLLHHAIKHEYGGWRVWVDTLAIVHSKVSYEEFKLQFAQMYEHYERQRTDNITDPALRQARPISTISGWEREELHQQQNGGSAAAVATNQTAAVKGSVSIASLEDVPPVVEEEVEELELEEVEIQEGPITEETEQKSVIANISDVYNEQLKTDATCNGNLEDVKEEEPVQQQIGDLEKQPEPSTPLGALRETLQLGDDMDVEELELATAKDALNAEQHVSRVLQASEAALNDCKMAVDDVLQESSSVLKDEEIELAVNEVVQGVLNNEKKTQSQDNKDNKEQPGEQDVNVSLLNSKNLLNNNNNNNNNSPSPTPTTATATAETEAETEVNANEIVSSTEAPKAETETSVAPEVETPETAKPSPIVPSPVLATNQKTEDAANKLNNNEKLAEISASPEPPIVVETPEADLLQLSDSETKPNKETEAEDSVALAVRDIVEQLIDKVIDATEAESASETKTETNNNEIPKKEKQTSEEPEDVETAETLAAAAKEIVQEVVEAALVMVQEESTQEKPEKGANSEEEKNEIGKEEILLQLEEKPASTEVQETKIEGDLKKPEDPKGHSSVEPKTPNLEEPKPQETEQQKSQEVAEELPQKPEEQVVAIVNQVLDTLVDDTVKAVAAEQTTQTSPAPEEQSPQILAMESPATSVRVKPTEVDSTTQTTPKNEAGSSLLVEQVQQVLQEDDAQQSAGMTIEDEDYSNQQAAAAVENANSSQLDANHYGPGNPESKQQQQRSKSGSTRPMFSPGPTRPPFRIPEFKWSYIHQRLLSDVLFSLETDIQVWRSHSTKSVLDFVNSSENAIFVVNTVHLISQLADNLIIACGGLLPLLASATSPNSELDVLEPTQGMPLEVAVSFLQRLVNMADVLIFATSLNFGELEAEKNMSSGGILRQCLRLVCTCAVRNCLECKERTRYNVGALARDVPGAAHLQALIRGAQASPKNIVESITGQLSPVKDPEKLLQDMDVNRLRAVIYRDVEETKQAQFLSLAIVYFISVLMVSKYRDILEPPAEPQIQRQSPVLQRTAGGGGRQIQDSDYEIIVVDENNPSVLADNDSHSSGPPSIKSVDSDVGSLNMNSTENEVPEVESSSEILIDDHKPSHSNDESWTDVNLNEDAAVQAASAGIVVGLVDNRGNVISDKHDPSHHNQQQQQQAGIIGQQQQHGSLGHSERGDKPDSEISVVRVPDGYGGAGSGGGVNSGQGQGVPSNQRPRPEELPMKAPALVAQLPLTTPSREASLTQKLEIALGPVCPLLREIMVDFAPFLSKTLVGSHGQELLMEGKGLTTFKNSHSVVELVMLLCSQEWQNSLQKHAGLAFIELINEGRLLSHAMKDHIVRVANEAEFILNRMRADDVLKHADFESQCAQTLLERREEERMCDHLITAARRRDNVIASRLLEKVRNIMCNRHGAWGDSSSTSSGGAIVGAVQKSPYWKLDAWEDDARRRKRMVQNPRGSSHPQATLKAALENGGPEDAILQTRDEFHTQIAVSRTHPSGQHNGELLDDAELLIEDRELDLDLTGPVNISTKARLIAPGLVAPGTVSITSTEMFFEVDEEHPEFQKIDGEVLKYCDHLHGKWYFSEVRAIFSRRYLLQNVALEIFLASRTSILFAFPDQHTVKKVIKALPRVGVGIKYGIPQTRRASMMSPRQLMRNSNMTQKWQRREISNFEYLMFLNTIAGRTYNDLNQYPIFPWVLTNYESKDLDLSLPSNYRDLSKPIGALNPSRRAYFEERYESWDSDTIPPFHYGTHYSTAAFTLNWLVRVEPFTTMFLALQGGKFDYPDRLFSSVSLSWKNCQRDTSDVKELIPEWYFLPEMFYNSSGYRLGHREDGALVDDIELPPWAKSPEEFVRINRMALESEFVSCQLHQWIDLIFGYKQRGPEAIRATNVFYYLTYEGSVDLDGVLDPVMREAVENQIRNFGQTPSQLLMEPHPPRSSAMHLSPMMFSAMPEDLCQMLKFYQNSPVIHISANTYPQLSLPSVVTVTAGHQFAVNRWNCNYTASVQSPSYAESPQSPGSNQPLTIDPVLAVHGTNNNSNAASRRHLGDNFSQMLKIRSNCFVTTVDSRFLIACGFWDNSFRVFATETAKIVQIVFGHFGVVTCMARSECNITSDCYIASGSADCTVLLWHWNARTQSIVGEGDVPTPRATLTGHEQAVTSVVISAELGLVVSGSSNGPVLIHTTFGDLLRSLDPPAEFHSPELITMSREGFIVINYDKGNVAAYTINGKKLRHETHNDNLQCMLLSRDGEYLMTAGDRGIVEVWRTFNLAPLYAFPACNAGIRSLALTHDQKYLLAGLSTGSIIVFHIDFNRWHHEYQQRY

Target ORF information:

RefSeq Version	NM_167028.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rugose (rg), transcript variant R, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167028.3

ORF Insert Sequence:

ATGGACTCGC TGGAGCGTCT GATGCGAGCG GCCCCATTGC CACGAATGCT TACCAGTGGC 
GTTGTGGCCA CAGCCGCTGC CGCCGCATCC GCCGCCGCTG GCAAGGGCAT GGTCAGTGTG 
GGCGGTGGTG CAACAGCCAT AGCCGCCGGC GGTGGCCAAC AGAGGGCGCT GGTCCATGCC 
TCGCTGGCGG CGGCGACAGT GGGCCGCAAG GGACGCCACC TAACGGGCAC CTTCTGCCTA 
ACCGGCGACA CCATGGAGGG CATCATACAG TGTTTGGTAT TTCTCAAAGC ATTCTCGCTG 
GTCGGCGGTG AATTCGATAT GGAATTGAAT TTTGTGATAC AGGATGCGCA GAATATCAAA 
CATATGCTGG AGCTGCTGGA TCATTGTCCG CCCAATTTGC AGGCGGAGAT TTGGAGCGTA 
TTCATCGCCA TCCTGCGCAA AAGCGTCCGC AATCTGCAGG CCTGCACCGA TGTTGGCCTA 
ATCGAGCATG TTCTCGTCCG CCTGCAACGC TCCGAAACGG TTGTGGCAGA CCTGCTCATC 
GAGATGTTGG GCGTGCTGGC CAGCTATAGC ATAACGGTGA AGGAGCTAAA GCTCCTCTTC 
GGTACGATGA AGGCCACCAA TGGCAAGTGG CCGCGCCACT CGGCCAAGTT GCTCAACGTC 
CTCCGGCAGA TGCCACATCG CAACGGACCA GACGTCTTCT TCAGCTTTCC GGGTCGCAAG 
GGATCGGCCA TGGTACTGCC GCCACTGGCC AAATGGCCCT ATGAGAACGG ATTCACCTTT 
ACCACCTGGT TCCGCCTGGA TCCCATCAAT TCGGTGAATA TTGAACGTGA GAAGCCCTAC 
CTCTACTGCT TCAAGACATC GAAGGGCGTG GGCTATACGG CGCATTTTGT GGGAAATTGC 
CTTGTCCTCA CCTCAATGAA GGTCAAGGGC AAAGGCTTTC AGCATTGTGT CAAATACGAA 
TTCCAGCCAC GAAAGTGGTA TATGATTGCC ATAGTGTATA TATACAATCG TTGGACGAAA 
AGCGAAATCA AGTGCCTCGT TAATGGACAG CTGGCCTCTT CAACGGAGAT GGCCTGGTTT 
GTTTCCACAA ACGATCCCTT TGACAAGTGC TACATTGGAG CCACCCCCGA GTTGGACGAG 
GAGCGCGTGT TCTGTGGCCA AATGTCGGCG ATCTACCTTT TCAGCGAGGC ACTGACCACG 
CAGCAGATCT GTGCGATGCA TCGCCTGGGT CCCGGCTACA AGTCCCAGTT CCGTTTCGAC 
AACGAGTGCT ATCTGAATCT GCCGGACAAT CACAAGCGGG TGCTGTACGA TGGCAAACTA 
TCGAATGCCA TTGTGTTCAT GTACAATCCG GTGGCCACCG ATGGTCAGTT GTGTCTGCAG 
TCATCGCCCA AGGGAAACGT TTCATATTTC GTGCACACGC CGCATGCGCT GATGTTGCAG 
GATGTGAAGG CCGTGGTCAC GCACTCGATA CACTGCACCC TCAATTCGAT TGGCGGCATC 
CAGGTGCTAT TCCCGCTTTT CTCGCAGCTG GATATGGCCC ACGAGGGTCT GGGCGACATT 
AAGCGGGATC CCACGCTGTG CTCCAAGCTG CTGGGCTTCA TCTGTGAACT GGTGGAAACA 
TCGCAGACGG TGCAGCAGCA TATGATCCAG AACCGGGGAT TCCTGGTCAT CTCGTTCATG 
CTGCAGCGCT CCTCCCGCGA ACACCTGACT CTCGAAGTCC TGGGATCATT CCTGAATCTG 
ACCAAGTATC TGGTCACCTG CCTGTCGGCC AACAGTGATC TGCTGCTCAA ACAGCTGCTG 
GATCACGTCC TCTTCAATCC AGCCCTGTGG ATATACACAC CCGCCAATGT CCAGGCGCGA 
CTATACTCCT ACTTGGCCAC CGAGTTCCTC TCGGACACGC AGATCTACAG CAATGTGAGG 
AGGGTCAGCA CGGTACTGCA GACAGTGCAC ACCCTAAAGT ACTACTACTG GGTGGTCAAT 
CCACGGGCTA AGAGCGGCAT CATTCCCAAG GGACTGGATG GACCTCGTCC GGCTCAAAAG 
GACATCCTGG CCATCCGGGC ATACATCCTG CTGTTCCTGA AGCAGCTCAT AATGATCGGC 
AATGGCGTGA AGGAGGACGA GCTGCAGAGC ATACTCAACT ATCTGACCAC CATGCACGAG 
GACGAGAACC TGCACGACGT GCTGCAGATG CTCATCTCGC TGATGTCGGA GCATCCCAGC 
TCCATGGTAC CTGCCTTTGA TGTGAAGCAC GGCGTGCGCA GCATCTTCAA GTTGCTGGCG 
GCCGAAAGTC AGTTGATTCG GCTTCAGGCC CTCAAGCTGC TGGGCTTCTT CCTTTCCCGC 
AGCACCCACA AACGCAAGTA CGATGTGATG TCACCACACA ATCTGTACAC CCTTTTAGCG 
GAGCGACTGC TTCTCTATGA GGAATCACTG TCCCTGCCCA CATACAATGT TCTCTATGAG 
ATCATGACGG AGCACATCTC GCAGCAGATT CTATACACAC GCCATCCGGA ACCAGAGAGT 
CACTACCGTC TGGAGAATCC AATGATCCTC AAGGTGGTGG CCACCCTGAT CCGGCAATCA 
AAGCAGACGG AATCGCTGAT CGACGTTAAA AAGCTATTCC TGCAGGATAT GACCCTGTTG 
TGCAATAGCA ATCGGGAGAA CCGGCGCACA GTGCTCCAGA TGTCCGTGTG GCAGGAGTGG 
CTCATTGCGA TGGCGTACAT TCATCCAAAG AGCAGCGAGG AGCAGAAGAT AAGCGACATG 
GTGTATTCCC TATTCCGCAT GCTGCTCCAC CATGCCATTA AGCATGAATA CGGTGGCTGG 
AGAGTTTGGG TAGATACCCT GGCAATCGTC CACTCCAAGG TGTCTTACGA GGAGTTTAAG 
CTCCAGTTTG CCCAGATGTA CGAGCACTAC GAGCGCCAGC GAACGGACAA TATCACAGAT 
CCTGCCCTGC GTCAGGCTAG ACCCATCAGC ACGATCAGTG GTTGGGAGCG AGAGGAGCTG 
CATCAGCAGC AGAACGGTGG ATCTGCAGCA GCAGTGGCTA CCAATCAAAC GGCAGCTGTT 
AAAGGTTCTG TTTCCATTGC CTCACTGGAA GATGTGCCAC CGGTGGTTGA GGAAGAGGTG 
GAGGAACTGG AGCTGGAGGA AGTAGAGATT CAAGAGGGTC CTATAACCGA AGAAACCGAA 
CAAAAATCGG TGATAGCCAA CATTTCCGAC GTCTACAATG AGCAGCTCAA AACCGATGCC 
ACGTGCAATG GCAATTTGGA GGATGTCAAA GAGGAGGAGC CTGTACAACA GCAAATCGGA 
GACCTGGAGA AGCAACCAGA GCCATCTACT CCTTTGGGAG CACTCAGGGA AACCCTACAA 
CTTGGTGATG ATATGGATGT GGAAGAACTG GAGCTGGCTA CCGCCAAGGA TGCCCTCAAT 
GCGGAACAGC ATGTGTCGCG AGTTCTTCAG GCTTCCGAAG CGGCACTTAA CGATTGCAAA 
ATGGCAGTCG ATGATGTCCT GCAGGAGTCA TCCTCCGTCC TCAAGGACGA GGAGATCGAA 
CTGGCCGTCA ACGAGGTTGT TCAGGGTGTG CTCAATAACG AGAAGAAAAC TCAATCCCAG 
GACAACAAGG ATAACAAGGA ACAGCCGGGC GAACAGGATG TAAACGTCAG TCTCCTAAAC 
AGCAAGAATC TGCTCAACAA TAATAATAAT AACAATAACA ACAGTCCGAG TCCAACGCCC 
ACAACGGCGA CTGCGACGGC GGAGACGGAA GCGGAAACAG AAGTCAATGC CAATGAGATC 
GTGAGCAGCA CAGAGGCGCC CAAGGCGGAA ACGGAAACGA GCGTAGCACC GGAAGTAGAA 
ACACCGGAAA CGGCCAAGCC AAGCCCCATT GTCCCCAGCC CCGTATTAGC AACCAATCAA 
AAGACTGAAG ATGCAGCCAA CAAGCTGAAC AACAACGAGA AGCTGGCCGA GATCAGCGCC 
AGTCCCGAGC CACCCATTGT CGTGGAAACG CCAGAAGCTG ATCTTCTTCA GCTTTCCGAT 
TCCGAAACAA AACCGAATAA GGAAACCGAA GCTGAAGATT CGGTGGCGCT GGCCGTTAGA 
GATATTGTCG AGCAACTCAT CGACAAGGTC ATCGATGCCA CGGAAGCGGA ATCGGCCAGC 
GAAACCAAAA CGGAAACGAA TAATAATGAA ATACCCAAGA AGGAGAAGCA GACGTCAGAA 
GAGCCTGAGG ATGTGGAAAC TGCAGAGACT TTGGCCGCTG CCGCCAAGGA AATTGTCCAA 
GAGGTTGTGG AAGCAGCTCT CGTAATGGTC CAAGAGGAGA GCACTCAAGA GAAGCCAGAA 
AAGGGGGCGA ACTCCGAAGA GGAGAAAAAC GAAATTGGCA AGGAAGAGAT TCTTCTGCAG 
CTGGAGGAAA AACCAGCGTC TACTGAAGTG CAGGAAACCA AGATCGAAGG AGATCTAAAG 
AAACCAGAAG ATCCAAAAGG TCACAGCTCT GTAGAACCAA AGACCCCGAA CCTTGAGGAA 
CCGAAACCCC AGGAAACTGA ACAACAAAAG TCGCAGGAGG TCGCCGAAGA ACTACCTCAA 
AAACCAGAGG AGCAGGTGGT GGCCATTGTC AACCAAGTCC TGGACACTCT GGTAGACGAT 
ACGGTCAAGG CAGTGGCAGC CGAGCAAACC ACTCAAACAT CGCCAGCCCC CGAAGAGCAA 
TCGCCACAAA TACTGGCAAT GGAATCACCA GCAACGTCGG TGCGGGTCAA ACCCACTGAG 
GTGGATTCCA CCACCCAGAC TACGCCGAAG AACGAGGCTG GATCCAGTCT ATTGGTTGAG 
CAGGTGCAGC AAGTCCTCCA GGAGGATGAC GCCCAGCAAT CAGCCGGCAT GACCATCGAA 
GATGAGGATT ACTCTAATCA GCAGGCAGCT GCGGCAGTTG AAAATGCCAA CAGCAGCCAA 
TTGGATGCCA ATCATTATGG TCCCGGTAAT CCAGAATCCA AACAACAGCA ACAGCGCTCC 
AAATCGGGAT CGACCCGACC TATGTTCAGT CCGGGACCTA CACGTCCGCC CTTCCGAATA 
CCGGAATTCA AGTGGTCCTA CATCCATCAG CGATTGCTCA GCGATGTCCT CTTTTCGCTG 
GAAACGGACA TTCAGGTGTG GCGCAGTCAT TCGACCAAAA GCGTCCTGGA CTTTGTCAAC 
TCCAGCGAGA ATGCAATTTT TGTGGTGAAC ACGGTGCATT TAATATCGCA GCTAGCGGAT 
AATCTGATTA TTGCCTGCGG AGGATTGCTG CCTCTTTTGG CCAGCGCCAC GTCACCCAAT 
TCCGAATTGG ATGTGCTGGA GCCAACGCAA GGCATGCCTT TGGAGGTGGC CGTATCTTTC 
CTGCAGCGTC TGGTCAACAT GGCGGATGTC CTGATCTTTG CCACATCCCT AAACTTCGGC 
GAGTTGGAGG CGGAGAAGAA CATGTCCAGT GGCGGCATAC TGCGTCAGTG CCTGCGATTG 
GTTTGCACCT GTGCGGTAAG GAATTGCTTG GAGTGCAAGG AGCGAACGCG TTACAATGTG 
GGCGCCTTGG CGAGAGATGT CCCGGGTGCA GCCCATTTGC AGGCCCTCAT CCGCGGAGCT 
CAAGCATCGC CCAAGAACAT TGTCGAGTCA ATAACCGGCC AATTATCGCC TGTCAAGGAT 
CCCGAGAAGC TGCTCCAGGA CATGGACGTC AATCGCCTGC GCGCAGTCAT CTATCGCGAT 
GTGGAGGAGA CTAAGCAGGC GCAGTTCCTC TCGCTGGCGA TAGTGTACTT CATATCGGTG 
CTGATGGTGT CCAAGTATCG TGATATCCTG GAGCCGCCGG CAGAGCCGCA AATCCAGCGT 
CAATCGCCAG TGCTGCAACG CACGGCAGGC GGCGGTGGTC GTCAAATCCA GGATAGTGAC 
TACGAAATAA TTGTCGTCGA TGAGAACAAT CCATCGGTTT TGGCGGATAA TGATTCACAT 
TCCAGTGGAC CACCATCCAT AAAGAGCGTT GACTCGGATG TGGGCTCCCT GAACATGAAC 
TCCACGGAAA ACGAAGTGCC CGAGGTGGAG TCATCCAGCG AGATTCTAAT CGATGACCAC 
AAACCGAGTC ACTCGAACGA CGAAAGCTGG ACGGATGTGA ATCTCAATGA GGATGCTGCC 
GTTCAGGCGG CAAGTGCCGG AATAGTTGTA GGTTTGGTGG ATAACAGAGG TAATGTTATA 
TCCGACAAAC ACGATCCGTC CCATCACAAC CAACAACAGC AGCAGCAGGC GGGAATTATT 
GGTCAGCAGC AGCAACATGG ATCACTTGGT CATTCGGAGC GGGGTGACAA ACCCGATTCG 
GAGATTTCGG TGGTGCGAGT GCCTGATGGT TATGGTGGTG CTGGTTCCGG TGGTGGCGTG 
AATTCCGGAC AAGGTCAGGG CGTTCCATCC AATCAGCGTC CTCGTCCCGA GGAGTTGCCC 
ATGAAGGCGC CCGCCTTGGT GGCCCAACTG CCGCTGACCA CACCTTCGCG AGAGGCCAGT 
CTCACCCAGA AGCTGGAAAT CGCATTGGGG CCAGTGTGTC CGCTGCTCCG TGAAATCATG 
GTGGACTTTG CGCCGTTCCT TTCGAAAACC CTAGTCGGCT CCCATGGCCA GGAATTGCTG 
ATGGAGGGCA AGGGTCTGAC GACCTTTAAG AACTCGCATT CCGTCGTAGA GCTGGTCATG 
TTGCTCTGCT CCCAGGAGTG GCAAAATAGC CTACAAAAGC ACGCAGGATT AGCCTTTATT 
GAGCTAATCA ATGAGGGTCG ACTGCTTTCG CACGCCATGA AGGATCACAT TGTTCGGGTG 
GCCAATGAGG CGGAGTTCAT CCTCAATAGA ATGCGAGCGG ATGATGTCCT CAAGCATGCC 
GACTTTGAAT CGCAATGTGC ACAGACACTT TTGGAGCGCA GGGAGGAGGA GCGAATGTGC 
GATCACTTGA TAACCGCCGC ACGTCGCAGA GACAATGTGA TTGCCAGCCG ACTGCTGGAG 
AAGGTACGAA ACATCATGTG TAATCGCCAC GGAGCCTGGG GTGATTCCAG CTCCACATCC 
AGTGGTGGCG CCATCGTTGG AGCGGTGCAA AAGAGTCCCT ACTGGAAGCT GGATGCCTGG 
GAGGATGATG CCAGACGTCG GAAACGGATG GTGCAAAATC CGCGCGGATC CTCGCATCCA 
CAGGCCACAC TGAAGGCGGC TTTGGAAAAT GGCGGACCTG AAGATGCCAT CCTGCAGACT 
CGCGACGAGT TCCACACCCA AATAGCCGTC TCGCGGACTC ATCCATCCGG TCAGCACAAT 
GGTGAGCTAC TGGACGACGC CGAGCTCTTG ATTGAGGATC GGGAGTTGGA TCTGGATCTC 
ACGGGTCCCG TCAACATTAG CACCAAGGCG AGATTGATAG CCCCCGGTCT GGTGGCACCC 
GGCACCGTCT CAATAACCAG CACTGAAATG TTCTTTGAGG TGGATGAAGA GCATCCCGAG 
TTCCAGAAGA TCGATGGCGA AGTTCTTAAG TACTGTGACC ATTTGCACGG CAAATGGTAC 
TTCTCCGAAG TGAGGGCCAT CTTCTCGAGG CGTTATCTCC TGCAAAATGT GGCCCTCGAG 
ATCTTTTTGG CCAGCCGTAC GTCCATATTG TTTGCCTTTC CCGATCAGCA TACGGTCAAG 
AAGGTAATCA AGGCCCTGCC CCGTGTGGGA GTGGGCATCA AGTATGGAAT ACCGCAGACG 
AGGAGGGCCT CAATGATGTC GCCCCGTCAA CTGATGCGCA ATTCGAACAT GACCCAAAAG 
TGGCAGCGTC GCGAGATTAG CAACTTTGAG TATCTTATGT TCCTGAACAC GATCGCCGGC 
AGGACGTACA ACGATCTAAA TCAGTATCCC ATCTTTCCGT GGGTGCTGAC CAACTATGAG 
TCCAAGGATT TGGATCTCAG CCTGCCGTCA AACTACAGGG ATCTGTCGAA ACCGATTGGC 
GCCCTGAATC CATCGCGCAG AGCCTATTTC GAGGAGCGTT ACGAGAGCTG GGACAGTGAC 
ACCATACCGC CCTTCCACTA TGGTACGCAT TATTCCACGG CAGCATTTAC GCTGAACTGG 
TTGGTGCGCG TGGAACCGTT TACCACCATG TTCCTGGCCC TGCAGGGCGG CAAATTTGAT 
TATCCGGACA GGCTGTTCAG CTCGGTGTCG CTGTCCTGGA AGAATTGCCA ACGTGATACA 
TCGGATGTGA AGGAACTGAT ACCAGAATGG TATTTCCTGC CCGAAATGTT TTACAATTCA 
TCAGGCTATC GACTGGGTCA TCGCGAGGAT GGTGCCCTAG TGGATGACAT TGAACTACCA 
CCGTGGGCCA AGAGCCCCGA AGAATTCGTC CGCATCAATC GCATGGCACT GGAGTCGGAG 
TTCGTGTCCT GCCAGCTGCA CCAGTGGATC GATTTGATCT TTGGCTACAA GCAACGCGGT 
CCCGAGGCCA TTAGGGCAAC CAACGTGTTC TACTATTTGA CATATGAGGG CAGCGTTGAC 
TTGGATGGCG TTTTGGATCC GGTGATGCGC GAAGCCGTCG AGAATCAAAT CCGCAACTTT 
GGCCAAACAC CTAGTCAACT GCTAATGGAA CCGCATCCGC CGCGCAGCTC TGCGATGCAT 
CTATCGCCTA TGATGTTCAG TGCGATGCCG GAGGATCTCT GCCAGATGCT CAAGTTCTAT 
CAGAACTCAC CAGTCATTCA CATCTCGGCC AACACCTATC CGCAACTGTC GTTGCCATCT 
GTGGTCACGG TAACGGCGGG TCATCAGTTT GCGGTGAATC GATGGAACTG CAACTACACC 
GCCTCCGTCC AGAGTCCCAG CTACGCCGAA TCGCCCCAAT CGCCGGGTTC CAATCAACCA 
CTGACCATCG ATCCGGTTCT GGCTGTTCAT GGCACCAACA ACAATAGCAA TGCGGCCAGC 
CGACGTCATT TGGGCGATAA CTTTAGCCAG ATGCTAAAGA TACGCTCCAA CTGCTTTGTT 
ACCACAGTGG ACAGTCGGTT CCTAATCGCA TGCGGATTCT GGGACAACAG TTTCCGCGTC 
TTTGCCACCG AAACGGCGAA AATCGTGCAG ATTGTATTCG GTCACTTTGG TGTGGTAACG 
TGCATGGCAC GTTCCGAGTG CAACATCACA TCAGACTGCT ACATCGCCTC CGGATCGGCG 
GACTGCACGG TGCTTCTGTG GCACTGGAAT GCCCGAACCC AGAGCATTGT GGGCGAGGGC 
GATGTGCCCA CACCACGGGC CACCTTGACG GGTCACGAAC AGGCCGTGAC ATCGGTGGTG 
ATTAGTGCCG AGTTGGGTCT GGTTGTGTCT GGATCATCAA ATGGACCCGT ACTTATCCAT 
ACGACTTTTG GCGACCTGCT GCGCTCACTG GATCCACCAG CGGAGTTCCA TTCCCCGGAA 
CTGATAACCA TGTCCCGCGA GGGTTTCATT GTCATCAACT ACGATAAGGG CAATGTGGCC 
GCCTACACCA TCAATGGCAA GAAACTGCGC CACGAGACGC ACAACGACAA TCTACAGTGC 
ATGCTACTGT CGCGCGATGG TGAATACCTG ATGACCGCCG GTGATCGCGG CATCGTGGAG 
GTGTGGCGCA CCTTCAACCT AGCACCACTT TATGCCTTCC CCGCATGCAA CGCGGGCATC 
CGATCTTTGG CTCTCACCCA CGATCAGAAA TACCTTCTGG CCGGACTTTC GACGGGCTCG 
ATCATAGTAT TCCACATCGA CTTCAATCGC TGGCACCACG AGTACCAGCA GCGCTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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