bon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	bon
Entrez Gene ID	44235
Full Name	bonus
Synonyms	0241/08,0249/12,0434/20,0487/06,150268_at,150269_at,Bon,Bonus,CG15687,CG5206,Dmel\CG5206,anon-WO0118547.418,l(3)S043420
General protein information	Preferred Names bonus Names CG5206 gene product from transcript CG5206-RA CG5206-PA CG5206-PB CG5206-PC CG5206-PD CG5206-PE CG5206-PF bon-PA bon-PB bon-PC bon-PD bon-PE bon-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F2-92F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170194.2	NP_001163665.1	bonus, isoform B
NM_001275836.1	NP_001262765.1	bonus, isoform C
NM_001275837.1	NP_001262766.1	bonus, isoform D
NM_001275838.1	NP_001262767.1	bonus, isoform E
NM_001275839.1	NP_001262768.1	bonus, isoform F
NM_079985.4	NP_524724.2	bonus, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that Fru forms a complex with the transcriptional cofactor Bonus (Bon), which, in turn, recruits either of two chromatin regulators, Histone deacetylase 1 (HDAC1), which masculinizes individual sexually dimorphic neurons, or Heterochromatin protein 1a (HP1a), which demasculinizes them.
Title: Fruitless recruits two antagonistic chromatin factors to establish single-neuron sexual dimorphism.

TRIM24 homolog, bonus, targets endogenous p53 for degradation.
Title: Trim24 targets endogenous p53 for degradation.

bon is a chromatin-associated protein that acts as a modifier of position-effect variegation.
Title: Bonus, a Drosophila TIF1 homolog, is a chromatin-associated protein that acts as a modifier of position-effect variegation.

The following bon gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bon cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06831	NM_079985.4 Latest version!	Drosophila melanogaster bonus (bon), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa08077	NM_001275836.1 Latest version!	Drosophila melanogaster bonus (bon), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OFa08078	NM_001275838.1 Latest version!	Drosophila melanogaster bonus (bon), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa08079	NM_001275837.1 Latest version!	Drosophila melanogaster bonus (bon), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa08080	NM_001275839.1 Latest version!	Drosophila melanogaster bonus (bon), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa08081	NM_001170194.2 Latest version!	Drosophila melanogaster bonus (bon), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bon ( NM_079985.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524724.2 Drosophila melanogaster bonus (bon), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06831
Clone ID Related Accession (Same CDS sequence)	NM_079985.4
Accession Version	NM_079985.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bonus, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079985.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATGTGAGT GCCATGCAGA ATTCGTTGGG GTTTCATGGG 
AGCCAGGCTG GCTTTAATAC CGGCCCACCA CAGACCTCCC CGCAGTTAGG CGGCGGAGGC 
ATGCACAGCC TGGCCAAGTG GCACATCCCG CAATCCGCGC AACAGTCGAA CATGTGTTCG 
CAACAAGGCC CCCTTTTGCC TTTTGCGAAT GGCCGCCAGA CATCGGAAAA TTTTAAAATC 
TCACTTAAAT CTCCCAACAC GCTCAAAAAT AGCACCCCGC CCAGCCTGGG GGGCGGTGGT 
GCGGGTCACC AGGGCCACGG AAACGGCTCC TCCAGCGCCC AACTGAACGC AGCTGCTCTG 
GGATTGGGGC CAGCCGTTTC GATACTCTCC AATGTCACCT CGACGAATCC AAAGACGCCC 
AGTCCCAGCA CTCATGAGAA CACCAAGGAC TTCACCGAAC CCATTGACAA GGTGCGAGAT 
GACTCAATCA ACGATCTAAT TGCAACCATA GCCAAACTGG ACTCGAATGG GGTACAGGTA 
TTGCCGGAGG GTCGGACAAA GACCACCTCG CCGCAGGTGC ACAGCTCGAC GGATCTGTCC 
AACACACAGG AAGTTAATAA TAAAAACGAA CAAAAAGATG ATCCCAACGA GGACTGGTGC 
GCCGTCTGTC TGGATGGAGG CGAGCTGATG TGCTGCGACA AGTGTCCCAA AGTTTTCCAT 
CAGAACTGTC ACATCCCTGC GATCAGCTCG TTGCCGGACG AGAGCGAGAG CTGGCAGTGC 
CTACTGTGCG TCAACATCAA GGAGCTGACA AAGACGGAGG GAAGTGAAAA GAGCTCCTCC 
GGCGAGCTGA GCGCCCTCGA ACTCCGCATC TTGCAGCGCA TCTGCCTAGA ATTGTACTGC 
CAGTACGAGC AGAGTCTCAA CTTCCGGGAG CCGGAGTCGC CAGCCAATAC ATCCTACTAC 
GAGATTGTTT CCAGTCCCAT GTCTCTAGAT GTTATTCGCA CCCGCCTGGA TCCATCCAGT 
CCGAATCACT ACAAGGACAT TGCAGGCTTC GTGTCCGACG TGCGTTTAAT ATTCTCCAAC 
ACATACCTCT TTTACCAGGA GGACACGAAA ACTTACTCCA ATGCCAAGTA CTTGGAAAAT 
TTCTTCGAGG AGCAGCTGGC CAAGTGGTTG CCGCAATTTG AGGGCACCAA GCCGCAGGGT 
AAGCGAAATA CCTCAAACTC GCCGGCGCTG TTAGGTGTGA ATGCAACTGG TTCGCCGTCG 
CCCATCGAGA ACGGACGGAA GAGCTGCGGC TCGGCATCAT TGGGTGACAG CGATGGTGCC 
TGCTTGCCGG CCAAGAGGGC GAGACGCTCG GCGCACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524724.2
CDS	706..4107
Translation	MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSGKVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNVSAMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEVNNKNEQKDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE

Target ORF information:

RefSeq Version	NM_079985.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bonus (bon), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079985.4

ORF Insert Sequence:

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATGTGAGT GCCATGCAGA ATTCGTTGGG GTTTCATGGG 
AGCCAGGCTG GCTTTAATAC CGGCCCACCA CAGACCTCCC CGCAGTTAGG CGGCGGAGGC 
ATGCACAGCC TGGCCAAGTG GCACATCCCG CAATCCGCGC AACAGTCGAA CATGTGTTCG 
CAACAAGGCC CCCTTTTGCC TTTTGCGAAT GGCCGCCAGA CATCGGAAAA TTTTAAAATC 
TCACTTAAAT CTCCCAACAC GCTCAAAAAT AGCACCCCGC CCAGCCTGGG GGGCGGTGGT 
GCGGGTCACC AGGGCCACGG AAACGGCTCC TCCAGCGCCC AACTGAACGC AGCTGCTCTG 
GGATTGGGGC CAGCCGTTTC GATACTCTCC AATGTCACCT CGACGAATCC AAAGACGCCC 
AGTCCCAGCA CTCATGAGAA CACCAAGGAC TTCACCGAAC CCATTGACAA GGTGCGAGAT 
GACTCAATCA ACGATCTAAT TGCAACCATA GCCAAACTGG ACTCGAATGG GGTACAGGTA 
TTGCCGGAGG GTCGGACAAA GACCACCTCG CCGCAGGTGC ACAGCTCGAC GGATCTGTCC 
AACACACAGG AAGTTAATAA TAAAAACGAA CAAAAAGATG ATCCCAACGA GGACTGGTGC 
GCCGTCTGTC TGGATGGAGG CGAGCTGATG TGCTGCGACA AGTGTCCCAA AGTTTTCCAT 
CAGAACTGTC ACATCCCTGC GATCAGCTCG TTGCCGGACG AGAGCGAGAG CTGGCAGTGC 
CTACTGTGCG TCAACATCAA GGAGCTGACA AAGACGGAGG GAAGTGAAAA GAGCTCCTCC 
GGCGAGCTGA GCGCCCTCGA ACTCCGCATC TTGCAGCGCA TCTGCCTAGA ATTGTACTGC 
CAGTACGAGC AGAGTCTCAA CTTCCGGGAG CCGGAGTCGC CAGCCAATAC ATCCTACTAC 
GAGATTGTTT CCAGTCCCAT GTCTCTAGAT GTTATTCGCA CCCGCCTGGA TCCATCCAGT 
CCGAATCACT ACAAGGACAT TGCAGGCTTC GTGTCCGACG TGCGTTTAAT ATTCTCCAAC 
ACATACCTCT TTTACCAGGA GGACACGAAA ACTTACTCCA ATGCCAAGTA CTTGGAAAAT 
TTCTTCGAGG AGCAGCTGGC CAAGTGGTTG CCGCAATTTG AGGGCACCAA GCCGCAGGGT 
AAGCGAAATA CCTCAAACTC GCCGGCGCTG TTAGGTGTGA ATGCAACTGG TTCGCCGTCG 
CCCATCGAGA ACGGACGGAA GAGCTGCGGC TCGGCATCAT TGGGTGACAG CGATGGTGCC 
TGCTTGCCGG CCAAGAGGGC GAGACGCTCG GCGCACGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bon ( NM_001275836.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262765.1 Drosophila melanogaster bonus (bon), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa08077
Clone ID Related Accession (Same CDS sequence)	NM_001275836.1
Accession Version	NM_001275836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bonus, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATGTGAGT GCCATGCAGA ATTCGTTGGG GTTTCATGGG 
AGCCAGGCTG GCTTTAATAC CGGCCCACCA CAGACCTCCC CGCAGTTAGG CGGCGGAGGC 
ATGCACAGCC TGGCCAAGTG GCACATCCCG CAATCCGCGC AACAGTCGAA CATGTGTTCG 
CAACAAGGCC CCCTTTTGCC TTTTGCGAAT GGCCGCCAGA CATCGGAAAA TTTTAAAATC 
TCACTTAAAT CTCCCAACAC GCTCAAAAAT AGCACCCCGC CCAGCCTGGG GGGCGGTGGT 
GCGGGTCACC AGGGCCACGG AAACGGCTCC TCCAGCGCCC AACTGAACGC AGCTGCTCTG 
GGATTGGGGC CAGCCGTTTC GATACTCTCC AATGTCACCT CGACGAATCC AAAGACGCCC 
AGTCCCAGCA CTCATGAGAA CACCAAGGAC TTCACCGAAC CCATTGACAA GGTGCGAGAT 
GACTCAATCA ACGATCTAAT TGCAACCATA GCCAAACTGG ACTCGAATGG GGTACAGGTA 
TTGCCGGAGG GTCGGACAAA GACCACCTCG CCGCAGGTGC ACAGCTCGAC GGATCTGTCC 
AACACACAGG AAGTTAATAA TAAAAACGAA CAAAAAGATG ATCCCAACGA GGACTGGTGC 
GCCGTCTGTC TGGATGGAGG CGAGCTGATG TGCTGCGACA AGTGTCCCAA AGTTTTCCAT 
CAGAACTGTC ACATCCCTGC GATCAGCTCG TTGCCGGACG AGAGCGAGAG CTGGCAGTGC 
CTACTGTGCG TCAACATCAA GGAGCTGACA AAGACGGAGG GAAGTGAAAA GAGCTCCTCC 
GGCGAGCTGA GCGCCCTCGA ACTCCGCATC TTGCAGCGCA TCTGCCTAGA ATTGTACTGC 
CAGTACGAGC AGAGTCTCAA CTTCCGGGAG CCGGAGTCGC CAGCCAATAC ATCCTACTAC 
GAGATTGTTT CCAGTCCCAT GTCTCTAGAT GTTATTCGCA CCCGCCTGGA TCCATCCAGT 
CCGAATCACT ACAAGGACAT TGCAGGCTTC GTGTCCGACG TGCGTTTAAT ATTCTCCAAC 
ACATACCTCT TTTACCAGGA GGACACGAAA ACTTACTCCA ATGCCAAGTA CTTGGAAAAT 
TTCTTCGAGG AGCAGCTGGC CAAGTGGTTG CCGCAATTTG AGGGCACCAA GCCGCAGGGT 
AAGCGAAATA CCTCAAACTC GCCGGCGCTG TTAGGTGTGA ATGCAACTGG TTCGCCGTCG 
CCCATCGAGA ACGGACGGAA GAGCTGCGGC TCGGCATCAT TGGGTGACAG CGATGGTGCC 
TGCTTGCCGG CCAAGAGGGC GAGACGCTCG GCGCACGAAT AGAACGAGCT AATGCCCGGC 
GGGAAGGGCA CAGATGGGGA GCAGCAGGCG GAGACGGGAC AAGAACGTCG CCAGGAGGAG 
CAGCAGCAAC TGCCCGCTTT CCTAGAGGAG ATGGACCTGG AGATGCCGCA ACGGCCGCAC 
AGCCTGGGCA GTAGTGTAAT CCAAGAGAGC GCCGATTATG TTCTTCAGTT ATTACACGCG 
TACGCTGCAG CCTTTGGTCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262765.1
CDS	706..4329
Translation	MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSGKVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNVSAMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEVNNKNEQKDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE*NELMPGGKGTDGEQQAETGQERRQEEQQQLPAFLEEMDLEMPQRPHSLGSSVIQESADYVLQLLHAYAAAFGL

Target ORF information:

RefSeq Version	NM_001275836.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bonus (bon), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275836.1

ORF Insert Sequence:

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATGTGAGT GCCATGCAGA ATTCGTTGGG GTTTCATGGG 
AGCCAGGCTG GCTTTAATAC CGGCCCACCA CAGACCTCCC CGCAGTTAGG CGGCGGAGGC 
ATGCACAGCC TGGCCAAGTG GCACATCCCG CAATCCGCGC AACAGTCGAA CATGTGTTCG 
CAACAAGGCC CCCTTTTGCC TTTTGCGAAT GGCCGCCAGA CATCGGAAAA TTTTAAAATC 
TCACTTAAAT CTCCCAACAC GCTCAAAAAT AGCACCCCGC CCAGCCTGGG GGGCGGTGGT 
GCGGGTCACC AGGGCCACGG AAACGGCTCC TCCAGCGCCC AACTGAACGC AGCTGCTCTG 
GGATTGGGGC CAGCCGTTTC GATACTCTCC AATGTCACCT CGACGAATCC AAAGACGCCC 
AGTCCCAGCA CTCATGAGAA CACCAAGGAC TTCACCGAAC CCATTGACAA GGTGCGAGAT 
GACTCAATCA ACGATCTAAT TGCAACCATA GCCAAACTGG ACTCGAATGG GGTACAGGTA 
TTGCCGGAGG GTCGGACAAA GACCACCTCG CCGCAGGTGC ACAGCTCGAC GGATCTGTCC 
AACACACAGG AAGTTAATAA TAAAAACGAA CAAAAAGATG ATCCCAACGA GGACTGGTGC 
GCCGTCTGTC TGGATGGAGG CGAGCTGATG TGCTGCGACA AGTGTCCCAA AGTTTTCCAT 
CAGAACTGTC ACATCCCTGC GATCAGCTCG TTGCCGGACG AGAGCGAGAG CTGGCAGTGC 
CTACTGTGCG TCAACATCAA GGAGCTGACA AAGACGGAGG GAAGTGAAAA GAGCTCCTCC 
GGCGAGCTGA GCGCCCTCGA ACTCCGCATC TTGCAGCGCA TCTGCCTAGA ATTGTACTGC 
CAGTACGAGC AGAGTCTCAA CTTCCGGGAG CCGGAGTCGC CAGCCAATAC ATCCTACTAC 
GAGATTGTTT CCAGTCCCAT GTCTCTAGAT GTTATTCGCA CCCGCCTGGA TCCATCCAGT 
CCGAATCACT ACAAGGACAT TGCAGGCTTC GTGTCCGACG TGCGTTTAAT ATTCTCCAAC 
ACATACCTCT TTTACCAGGA GGACACGAAA ACTTACTCCA ATGCCAAGTA CTTGGAAAAT 
TTCTTCGAGG AGCAGCTGGC CAAGTGGTTG CCGCAATTTG AGGGCACCAA GCCGCAGGGT 
AAGCGAAATA CCTCAAACTC GCCGGCGCTG TTAGGTGTGA ATGCAACTGG TTCGCCGTCG 
CCCATCGAGA ACGGACGGAA GAGCTGCGGC TCGGCATCAT TGGGTGACAG CGATGGTGCC 
TGCTTGCCGG CCAAGAGGGC GAGACGCTCG GCGCACGAAT AGAACGAGCT AATGCCCGGC 
GGGAAGGGCA CAGATGGGGA GCAGCAGGCG GAGACGGGAC AAGAACGTCG CCAGGAGGAG 
CAGCAGCAAC TGCCCGCTTT CCTAGAGGAG ATGGACCTGG AGATGCCGCA ACGGCCGCAC 
AGCCTGGGCA GTAGTGTAAT CCAAGAGAGC GCCGATTATG TTCTTCAGTT ATTACACGCG 
TACGCTGCAG CCTTTGGTCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bon ( NM_001275838.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262767.1 Drosophila melanogaster bonus (bon), transcript variant E, mRNA.

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CloneID	OFa08078
Clone ID Related Accession (Same CDS sequence)	NM_001275838.1
Accession Version	NM_001275838.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bonus, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATGTGAGT GCCATGCAGA ATTCGTTGGG GTTTCATGGG 
AGCCAGGCTG GCTTTAATAC CGGCCCACCA CAGACCTCCC CGCAGTTAGG CGGCGGAGGC 
ATGCACAGCC TGGCCAAGTG GCACATCCCG CAATCCGCGC AACAGTCGAA CATGTGTTCG 
CAACAAGGCC CCCTTTTGCC TTTTGCGAAT GGCCGCCAGA CATCGGAAAA TTTTAAAATC 
TCACTTAAAT CTCCCAACAC GCTCAAAAAT AGCACCCCGC CCAGCCTGGG GGGCGGTGGT 
GCGGGTCACC AGGGCCACGG AAACGGCTCC TCCAGCGCCC AACTGAACGC AGCTGCTCTG 
GGATTGGGGC CAGCCGTTTC GATACTCTCC AATGTCACCT CGACGAATCC AAAGACGCCC 
AGTCCCAGCA CTCATGAGAA CACCAAGGAC TTCACCGAAC CCATTGACAA GGTGCGAGAT 
GACTCAATCA ACGATCTAAT TGCAACCATA GCCAAACTGG ACTCGAATGG GGTACAGGTA 
TTGCCGGAGG GTCGGACAAA GACCACCTCG CCGCAGGTGC ACAGCTCGAC GGATCTGTCC 
AACACACAGG AAGATGATCC CAACGAGGAC TGGTGCGCCG TCTGTCTGGA TGGAGGCGAG 
CTGATGTGCT GCGACAAGTG TCCCAAAGTT TTCCATCAGA ACTGTCACAT CCCTGCGATC 
AGCTCGTTGC CGGACGAGAG CGAGAGCTGG CAGTGCCTAC TGTGCGTCAA CATCAAGGAG 
CTGACAAAGA CGGAGGGAAG TGAAAAGAGC TCCTCCGGCG AGCTGAGCGC CCTCGAACTC 
CGCATCTTGC AGCGCATCTG CCTAGAATTG TACTGCCAGT ACGAGCAGAG TCTCAACTTC 
CGGGAGCCGG AGTCGCCAGC CAATACATCC TACTACGAGA TTGTTTCCAG TCCCATGTCT 
CTAGATGTTA TTCGCACCCG CCTGGATCCA TCCAGTCCGA ATCACTACAA GGACATTGCA 
GGCTTCGTGT CCGACGTGCG TTTAATATTC TCCAACACAT ACCTCTTTTA CCAGGAGGAC 
ACGAAAACTT ACTCCAATGC CAAGTACTTG GAAAATTTCT TCGAGGAGCA GCTGGCCAAG 
TGGTTGCCGC AATTTGAGGG CACCAAGCCG CAGGGTAAGC GAAATACCTC AAACTCGCCG 
GCGCTGTTAG GTGTGAATGC AACTGGTTCG CCGTCGCCCA TCGAGAACGG ACGGAAGAGC 
TGCGGCTCGG CATCATTGGG TGACAGCGAT GGTGCCTGCT TGCCGGCCAA GAGGGCGAGA 
CGCTCGGCGC ACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262767.1
CDS	706..4083
Translation	MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSGKVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNVSAMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE

Target ORF information:

RefSeq Version	NM_001275838.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bonus (bon), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275838.1

ORF Insert Sequence:

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATGTGAGT GCCATGCAGA ATTCGTTGGG GTTTCATGGG 
AGCCAGGCTG GCTTTAATAC CGGCCCACCA CAGACCTCCC CGCAGTTAGG CGGCGGAGGC 
ATGCACAGCC TGGCCAAGTG GCACATCCCG CAATCCGCGC AACAGTCGAA CATGTGTTCG 
CAACAAGGCC CCCTTTTGCC TTTTGCGAAT GGCCGCCAGA CATCGGAAAA TTTTAAAATC 
TCACTTAAAT CTCCCAACAC GCTCAAAAAT AGCACCCCGC CCAGCCTGGG GGGCGGTGGT 
GCGGGTCACC AGGGCCACGG AAACGGCTCC TCCAGCGCCC AACTGAACGC AGCTGCTCTG 
GGATTGGGGC CAGCCGTTTC GATACTCTCC AATGTCACCT CGACGAATCC AAAGACGCCC 
AGTCCCAGCA CTCATGAGAA CACCAAGGAC TTCACCGAAC CCATTGACAA GGTGCGAGAT 
GACTCAATCA ACGATCTAAT TGCAACCATA GCCAAACTGG ACTCGAATGG GGTACAGGTA 
TTGCCGGAGG GTCGGACAAA GACCACCTCG CCGCAGGTGC ACAGCTCGAC GGATCTGTCC 
AACACACAGG AAGATGATCC CAACGAGGAC TGGTGCGCCG TCTGTCTGGA TGGAGGCGAG 
CTGATGTGCT GCGACAAGTG TCCCAAAGTT TTCCATCAGA ACTGTCACAT CCCTGCGATC 
AGCTCGTTGC CGGACGAGAG CGAGAGCTGG CAGTGCCTAC TGTGCGTCAA CATCAAGGAG 
CTGACAAAGA CGGAGGGAAG TGAAAAGAGC TCCTCCGGCG AGCTGAGCGC CCTCGAACTC 
CGCATCTTGC AGCGCATCTG CCTAGAATTG TACTGCCAGT ACGAGCAGAG TCTCAACTTC 
CGGGAGCCGG AGTCGCCAGC CAATACATCC TACTACGAGA TTGTTTCCAG TCCCATGTCT 
CTAGATGTTA TTCGCACCCG CCTGGATCCA TCCAGTCCGA ATCACTACAA GGACATTGCA 
GGCTTCGTGT CCGACGTGCG TTTAATATTC TCCAACACAT ACCTCTTTTA CCAGGAGGAC 
ACGAAAACTT ACTCCAATGC CAAGTACTTG GAAAATTTCT TCGAGGAGCA GCTGGCCAAG 
TGGTTGCCGC AATTTGAGGG CACCAAGCCG CAGGGTAAGC GAAATACCTC AAACTCGCCG 
GCGCTGTTAG GTGTGAATGC AACTGGTTCG CCGTCGCCCA TCGAGAACGG ACGGAAGAGC 
TGCGGCTCGG CATCATTGGG TGACAGCGAT GGTGCCTGCT TGCCGGCCAA GAGGGCGAGA 
CGCTCGGCGC ACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bon ( NM_001275837.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262766.1 Drosophila melanogaster bonus (bon), transcript variant D, mRNA.

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CloneID	OFa08079
Clone ID Related Accession (Same CDS sequence)	NM_001275837.1
Accession Version	NM_001275837.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bonus, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATTCGTTG GGGTTTCATG GGAGCCAGGC TGGCTTTAAT 
ACCGGCCCAC CACAGACCTC CCCGCAGTTA GGCGGCGGAG GCATGCACAG CCTGGCCAAG 
TGGCACATCC CGCAATCCGC GCAACAGTCG AACATGTGTT CGCAACAAGG CCCCCTTTTG 
CCTTTTGCGA ATGGCCGCCA GACATCGGAA AATTTTAAAA TCTCACTTAA ATCTCCCAAC 
ACGCTCAAAA ATAGCACCCC GCCCAGCCTG GGGGGCGGTG GTGCGGGTCA CCAGGGCCAC 
GGAAACGGCT CCTCCAGCGC CCAACTGAAC GCAGCTGCTC TGGGATTGGG GCCAGCCGTT 
TCGATACTCT CCAATGTCAC CTCGACGAAT CCAAAGACGC CCAGTCCCAG CACTCATGAG 
AACACCAAGG ACTTCACCGA ACCCATTGAC AAGGTGCGAG ATGACTCAAT CAACGATCTA 
ATTGCAACCA TAGCCAAACT GGACTCGAAT GGGGTACAGG TATTGCCGGA GGGTCGGACA 
AAGACCACCT CGCCGCAGGT GCACAGCTCG ACGGATCTGT CCAACACACA GGAAGATGAT 
CCCAACGAGG ACTGGTGCGC CGTCTGTCTG GATGGAGGCG AGCTGATGTG CTGCGACAAG 
TGTCCCAAAG TTTTCCATCA GAACTGTCAC ATCCCTGCGA TCAGCTCGTT GCCGGACGAG 
AGCGAGAGCT GGCAGTGCCT ACTGTGCGTC AACATCAAGG AGCTGACAAA GACGGAGGGA 
AGTGAAAAGA GCTCCTCCGG CGAGCTGAGC GCCCTCGAAC TCCGCATCTT GCAGCGCATC 
TGCCTAGAAT TGTACTGCCA GTACGAGCAG AGTCTCAACT TCCGGGAGCC GGAGTCGCCA 
GCCAATACAT CCTACTACGA GATTGTTTCC AGTCCCATGT CTCTAGATGT TATTCGCACC 
CGCCTGGATC CATCCAGTCC GAATCACTAC AAGGACATTG CAGGCTTCGT GTCCGACGTG 
CGTTTAATAT TCTCCAACAC ATACCTCTTT TACCAGGAGG ACACGAAAAC TTACTCCAAT 
GCCAAGTACT TGGAAAATTT CTTCGAGGAG CAGCTGGCCA AGTGGTTGCC GCAATTTGAG 
GGCACCAAGC CGCAGGGTAA GCGAAATACC TCAAACTCGC CGGCGCTGTT AGGTGTGAAT 
GCAACTGGTT CGCCGTCGCC CATCGAGAAC GGACGGAAGA GCTGCGGCTC GGCATCATTG 
GGTGACAGCG ATGGTGCCTG CTTGCCGGCC AAGAGGGCGA GACGCTCGGC GCACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262766.1
CDS	706..4065
Translation	MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSGKVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE

Target ORF information:

RefSeq Version	NM_001275837.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bonus (bon), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275837.1

ORF Insert Sequence:

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGGT AAGGTTCGGT TCGGGGGAAT GCCGCCAGTG 
GGCATGCAGC GACACGGACA ACCGCACGTG AGCTCCTCCA CACATCCGCA GAATATGGAC 
ATCAGCCTGC GGGGCCTACT GAACAACCAG GCGGCGCAGA GCCCGAATGC CCACATGGCA 
TTTAATGGAC CGCCCAGCTA TCCGGGTGGG CCGCAAGGAG CACCGGCTCC GGCCCACCAA 
CCGATGGGCC CGCAGATGCG TCCGCATTTC ATGCCCGGCC AGCAGGGTTT CTCGCAGGGC 
GGTGGACCAG GAGGCGGTCC GCGGGACGCC AACTTTATGA ACAGCAACGC GCGCTTCCAG 
TCGCAGTATC AGCGTATGGC CAACCACGCT CAGCAGGCGG CGGCTGCGGC GGCCATGGCC 
GGTGCGGCGG GGGGCGGAGG CGGCCAAATC CCTTCGCCGG GTGCACTGCA GCGACCGCAG 
ATGATGTCCA ATCCCATGCA GAATTCGTTG GGGTTTCATG GGAGCCAGGC TGGCTTTAAT 
ACCGGCCCAC CACAGACCTC CCCGCAGTTA GGCGGCGGAG GCATGCACAG CCTGGCCAAG 
TGGCACATCC CGCAATCCGC GCAACAGTCG AACATGTGTT CGCAACAAGG CCCCCTTTTG 
CCTTTTGCGA ATGGCCGCCA GACATCGGAA AATTTTAAAA TCTCACTTAA ATCTCCCAAC 
ACGCTCAAAA ATAGCACCCC GCCCAGCCTG GGGGGCGGTG GTGCGGGTCA CCAGGGCCAC 
GGAAACGGCT CCTCCAGCGC CCAACTGAAC GCAGCTGCTC TGGGATTGGG GCCAGCCGTT 
TCGATACTCT CCAATGTCAC CTCGACGAAT CCAAAGACGC CCAGTCCCAG CACTCATGAG 
AACACCAAGG ACTTCACCGA ACCCATTGAC AAGGTGCGAG ATGACTCAAT CAACGATCTA 
ATTGCAACCA TAGCCAAACT GGACTCGAAT GGGGTACAGG TATTGCCGGA GGGTCGGACA 
AAGACCACCT CGCCGCAGGT GCACAGCTCG ACGGATCTGT CCAACACACA GGAAGATGAT 
CCCAACGAGG ACTGGTGCGC CGTCTGTCTG GATGGAGGCG AGCTGATGTG CTGCGACAAG 
TGTCCCAAAG TTTTCCATCA GAACTGTCAC ATCCCTGCGA TCAGCTCGTT GCCGGACGAG 
AGCGAGAGCT GGCAGTGCCT ACTGTGCGTC AACATCAAGG AGCTGACAAA GACGGAGGGA 
AGTGAAAAGA GCTCCTCCGG CGAGCTGAGC GCCCTCGAAC TCCGCATCTT GCAGCGCATC 
TGCCTAGAAT TGTACTGCCA GTACGAGCAG AGTCTCAACT TCCGGGAGCC GGAGTCGCCA 
GCCAATACAT CCTACTACGA GATTGTTTCC AGTCCCATGT CTCTAGATGT TATTCGCACC 
CGCCTGGATC CATCCAGTCC GAATCACTAC AAGGACATTG CAGGCTTCGT GTCCGACGTG 
CGTTTAATAT TCTCCAACAC ATACCTCTTT TACCAGGAGG ACACGAAAAC TTACTCCAAT 
GCCAAGTACT TGGAAAATTT CTTCGAGGAG CAGCTGGCCA AGTGGTTGCC GCAATTTGAG 
GGCACCAAGC CGCAGGGTAA GCGAAATACC TCAAACTCGC CGGCGCTGTT AGGTGTGAAT 
GCAACTGGTT CGCCGTCGCC CATCGAGAAC GGACGGAAGA GCTGCGGCTC GGCATCATTG 
GGTGACAGCG ATGGTGCCTG CTTGCCGGCC AAGAGGGCGA GACGCTCGGC GCACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bon ( NM_001275839.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262768.1 Drosophila melanogaster bonus (bon), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa08080
Clone ID Related Accession (Same CDS sequence)	NM_001275839.1
Accession Version	NM_001275839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bonus, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGTT CGGTTCGGGG GAATGCCGCC AGTGGGCATG 
CAGCGACACG GACAACCGCA CGTGAGCTCC TCCACACATC CGCAGAATAT GGACATCAGC 
CTGCGGGGCC TACTGAACAA CCAGGCGGCG CAGAGCCCGA ATGCCCACAT GGCATTTAAT 
GGACCGCCCA GCTATCCGGG TGGGCCGCAA GGAGCACCGG CTCCGGCCCA CCAACCGATG 
GGCCCGCAGA TGCGTCCGCA TTTCATGCCC GGCCAGCAGG GTTTCTCGCA GGGCGGTGGA 
CCAGGAGGCG GTCCGCGGGA CGCCAACTTT ATGAACAGCA ACGCGCGCTT CCAGTCGCAG 
TATCAGCGTA TGGCCAACCA CGCTCAGCAG GCGGCGGCTG CGGCGGCCAT GGCCGGTGCG 
GCGGGGGGCG GAGGCGGCCA AATCCCTTCG CCGGGTGCAC TGCAGCGACC GCAGATGATG 
TCCAATCCCA TGCAGAATGT GAGTGCCATG CAGAATTCGT TGGGGTTTCA TGGGAGCCAG 
GCTGGCTTTA ATACCGGCCC ACCACAGACC TCCCCGCAGT TAGGCGGCGG AGGCATGCAC 
AGCCTGGCCA AGTGGCACAT CCCGCAATCC GCGCAACAGT CGAACATGTG TTCGCAACAA 
GGCCCCCTTT TGCCTTTTGC GAATGGCCGC CAGACATCGG AAAATTTTAA AATCTCACTT 
AAATCTCCCA ACACGCTCAA AAATAGCACC CCGCCCAGCC TGGGGGGCGG TGGTGCGGGT 
CACCAGGGCC ACGGAAACGG CTCCTCCAGC GCCCAACTGA ACGCAGCTGC TCTGGGATTG 
GGGCCAGCCG TTTCGATACT CTCCAATGTC ACCTCGACGA ATCCAAAGAC GCCCAGTCCC 
AGCACTCATG AGAACACCAA GGACTTCACC GAACCCATTG ACAAGGTGCG AGATGACTCA 
ATCAACGATC TAATTGCAAC CATAGCCAAA CTGGACTCGA ATGGGGTACA GGTATTGCCG 
GAGGGTCGGA CAAAGACCAC CTCGCCGCAG GTGCACAGCT CGACGGATCT GTCCAACACA 
CAGGAAGATG ATCCCAACGA GGACTGGTGC GCCGTCTGTC TGGATGGAGG CGAGCTGATG 
TGCTGCGACA AGTGTCCCAA AGTTTTCCAT CAGAACTGTC ACATCCCTGC GATCAGCTCG 
TTGCCGGACG AGAGCGAGAG CTGGCAGTGC CTACTGTGCG TCAACATCAA GGAGCTGACA 
AAGACGGAGG GAAGTGAAAA GAGCTCCTCC GGCGAGCTGA GCGCCCTCGA ACTCCGCATC 
TTGCAGCGCA TCTGCCTAGA ATTGTACTGC CAGTACGAGC AGAGTCTCAA CTTCCGGGAG 
CCGGAGTCGC CAGCCAATAC ATCCTACTAC GAGATTGTTT CCAGTCCCAT GTCTCTAGAT 
GTTATTCGCA CCCGCCTGGA TCCATCCAGT CCGAATCACT ACAAGGACAT TGCAGGCTTC 
GTGTCCGACG TGCGTTTAAT ATTCTCCAAC ACATACCTCT TTTACCAGGA CACGAAAACT 
TACTCCAATG CCAAGTACTT GGAAAATTTC TTCGAGGAGC AGCTGGCCAA GTGGTTGCCG 
CAATTTGAGG GCACCAAGCC GCAGGGTAAG CGAAATACCT CAAACTCGCC GGCGCTGTTA 
GGTGTGAATG CAACTGGTTC GCCGTCGCCC ATCGAGAACG GACGGAAGAG CTGCGGCTCG 
GCATCATTGG GTGACAGCGA TGGTGCCTGC TTGCCGGCCA AGAGGGCGAG ACGCTCGGCG 
CACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262768.1
CDS	706..4074
Translation	MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNVSAMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE

Target ORF information:

RefSeq Version	NM_001275839.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bonus (bon), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275839.1

ORF Insert Sequence:

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGTT CGGTTCGGGG GAATGCCGCC AGTGGGCATG 
CAGCGACACG GACAACCGCA CGTGAGCTCC TCCACACATC CGCAGAATAT GGACATCAGC 
CTGCGGGGCC TACTGAACAA CCAGGCGGCG CAGAGCCCGA ATGCCCACAT GGCATTTAAT 
GGACCGCCCA GCTATCCGGG TGGGCCGCAA GGAGCACCGG CTCCGGCCCA CCAACCGATG 
GGCCCGCAGA TGCGTCCGCA TTTCATGCCC GGCCAGCAGG GTTTCTCGCA GGGCGGTGGA 
CCAGGAGGCG GTCCGCGGGA CGCCAACTTT ATGAACAGCA ACGCGCGCTT CCAGTCGCAG 
TATCAGCGTA TGGCCAACCA CGCTCAGCAG GCGGCGGCTG CGGCGGCCAT GGCCGGTGCG 
GCGGGGGGCG GAGGCGGCCA AATCCCTTCG CCGGGTGCAC TGCAGCGACC GCAGATGATG 
TCCAATCCCA TGCAGAATGT GAGTGCCATG CAGAATTCGT TGGGGTTTCA TGGGAGCCAG 
GCTGGCTTTA ATACCGGCCC ACCACAGACC TCCCCGCAGT TAGGCGGCGG AGGCATGCAC 
AGCCTGGCCA AGTGGCACAT CCCGCAATCC GCGCAACAGT CGAACATGTG TTCGCAACAA 
GGCCCCCTTT TGCCTTTTGC GAATGGCCGC CAGACATCGG AAAATTTTAA AATCTCACTT 
AAATCTCCCA ACACGCTCAA AAATAGCACC CCGCCCAGCC TGGGGGGCGG TGGTGCGGGT 
CACCAGGGCC ACGGAAACGG CTCCTCCAGC GCCCAACTGA ACGCAGCTGC TCTGGGATTG 
GGGCCAGCCG TTTCGATACT CTCCAATGTC ACCTCGACGA ATCCAAAGAC GCCCAGTCCC 
AGCACTCATG AGAACACCAA GGACTTCACC GAACCCATTG ACAAGGTGCG AGATGACTCA 
ATCAACGATC TAATTGCAAC CATAGCCAAA CTGGACTCGA ATGGGGTACA GGTATTGCCG 
GAGGGTCGGA CAAAGACCAC CTCGCCGCAG GTGCACAGCT CGACGGATCT GTCCAACACA 
CAGGAAGATG ATCCCAACGA GGACTGGTGC GCCGTCTGTC TGGATGGAGG CGAGCTGATG 
TGCTGCGACA AGTGTCCCAA AGTTTTCCAT CAGAACTGTC ACATCCCTGC GATCAGCTCG 
TTGCCGGACG AGAGCGAGAG CTGGCAGTGC CTACTGTGCG TCAACATCAA GGAGCTGACA 
AAGACGGAGG GAAGTGAAAA GAGCTCCTCC GGCGAGCTGA GCGCCCTCGA ACTCCGCATC 
TTGCAGCGCA TCTGCCTAGA ATTGTACTGC CAGTACGAGC AGAGTCTCAA CTTCCGGGAG 
CCGGAGTCGC CAGCCAATAC ATCCTACTAC GAGATTGTTT CCAGTCCCAT GTCTCTAGAT 
GTTATTCGCA CCCGCCTGGA TCCATCCAGT CCGAATCACT ACAAGGACAT TGCAGGCTTC 
GTGTCCGACG TGCGTTTAAT ATTCTCCAAC ACATACCTCT TTTACCAGGA CACGAAAACT 
TACTCCAATG CCAAGTACTT GGAAAATTTC TTCGAGGAGC AGCTGGCCAA GTGGTTGCCG 
CAATTTGAGG GCACCAAGCC GCAGGGTAAG CGAAATACCT CAAACTCGCC GGCGCTGTTA 
GGTGTGAATG CAACTGGTTC GCCGTCGCCC ATCGAGAACG GACGGAAGAG CTGCGGCTCG 
GCATCATTGG GTGACAGCGA TGGTGCCTGC TTGCCGGCCA AGAGGGCGAG ACGCTCGGCG 
CACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bon ( NM_001170194.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163665.1 Drosophila melanogaster bonus (bon), transcript variant B, mRNA.

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CloneID	OFa08081
Clone ID Related Accession (Same CDS sequence)	NM_001170194.2
Accession Version	NM_001170194.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	bonus, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170194.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGTT CGGTTCGGGG GAATGCCGCC AGTGGGCATG 
CAGCGACACG GACAACCGCA CGTGAGCTCC TCCACACATC CGCAGAATAT GGACATCAGC 
CTGCGGGGCC TACTGAACAA CCAGGCGGCG CAGAGCCCGA ATGCCCACAT GGCATTTAAT 
GGACCGCCCA GCTATCCGGG TGGGCCGCAA GGAGCACCGG CTCCGGCCCA CCAACCGATG 
GGCCCGCAGA TGCGTCCGCA TTTCATGCCC GGCCAGCAGG GTTTCTCGCA GGGCGGTGGA 
CCAGGAGGCG GTCCGCGGGA CGCCAACTTT ATGAACAGCA ACGCGCGCTT CCAGTCGCAG 
TATCAGCGTA TGGCCAACCA CGCTCAGCAG GCGGCGGCTG CGGCGGCCAT GGCCGGTGCG 
GCGGGGGGCG GAGGCGGCCA AATCCCTTCG CCGGGTGCAC TGCAGCGACC GCAGATGATG 
TCCAATCCCA TGCAGAATTC GTTGGGGTTT CATGGGAGCC AGGCTGGCTT TAATACCGGC 
CCACCACAGA CCTCCCCGCA GTTAGGCGGC GGAGGCATGC ACAGCCTGGC CAAGTGGCAC 
ATCCCGCAAT CCGCGCAACA GTCGAACATG TGTTCGCAAC AAGGCCCCCT TTTGCCTTTT 
GCGAATGGCC GCCAGACATC GGAAAATTTT AAAATCTCAC TTAAATCTCC CAACACGCTC 
AAAAATAGCA CCCCGCCCAG CCTGGGGGGC GGTGGTGCGG GTCACCAGGG CCACGGAAAC 
GGCTCCTCCA GCGCCCAACT GAACGCAGCT GCTCTGGGAT TGGGGCCAGC CGTTTCGATA 
CTCTCCAATG TCACCTCGAC GAATCCAAAG ACGCCCAGTC CCAGCACTCA TGAGAACACC 
AAGGACTTCA CCGAACCCAT TGACAAGGTG CGAGATGACT CAATCAACGA TCTAATTGCA 
ACCATAGCCA AACTGGACTC GAATGGGGTA CAGGTATTGC CGGAGGGTCG GACAAAGACC 
ACCTCGCCGC AGGTGCACAG CTCGACGGAT CTGTCCAACA CACAGGAAGT TAATAATAAA 
AACGAACAAA AAGATGATCC CAACGAGGAC TGGTGCGCCG TCTGTCTGGA TGGAGGCGAG 
CTGATGTGCT GCGACAAGTG TCCCAAAGTT TTCCATCAGA ACTGTCACAT CCCTGCGATC 
AGCTCGTTGC CGGACGAGAG CGAGAGCTGG CAGTGCCTAC TGTGCGTCAA CATCAAGGAG 
CTGACAAAGA CGGAGGGAAG TGAAAAGAGC TCCTCCGGCG AGCTGAGCGC CCTCGAACTC 
CGCATCTTGC AGCGCATCTG CCTAGAATTG TACTGCCAGT ACGAGCAGAG TCTCAACTTC 
CGGGAGCCGG AGTCGCCAGC CAATACATCC TACTACGAGA TTGTTTCCAG TCCCATGTCT 
CTAGATGTTA TTCGCACCCG CCTGGATCCA TCCAGTCCGA ATCACTACAA GGACATTGCA 
GGCTTCGTGT CCGACGTGCG TTTAATATTC TCCAACACAT ACCTCTTTTA CCAGGAGGAC 
ACGAAAACTT ACTCCAATGC CAAGTACTTG GAAAATTTCT TCGAGGAGCA GCTGGCCAAG 
TGGTTGCCGC AATTTGAGGG CACCAAGCCG CAGGGTAAGC GAAATACCTC AAACTCGCCG 
GCGCTGTTAG GTGTGAATGC AACTGGTTCG CCGTCGCCCA TCGAGAACGG ACGGAAGAGC 
TGCGGCTCGG CATCATTGGG TGACAGCGAT GGTGCCTGCT TGCCGGCCAA GAGGGCGAGA 
CGCTCGGCGC ACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163665.1
CDS	706..4083
Translation	MDMDLEQLKNDFLPLIAGIKQEQLDAVPTDALPQMSTPNTASGAPTTSSLSSSSLSLSNPCDSAEKRSESNSSASAKFTLFKCVYCAQLLGSNDRPKLLECLHVACAQCVSTKFSELDRSLPPLIHCPVCDNASQQEFIVDNQFLIEQCTAGDSGDGVGLLGLTGEGQKSSAAASIQCSSCSDGAVATSWCVDCSEYICDSCVQAHQRLKITKDHTIKPKDEANNEQLAGAAGVDKLHMCQLHPQEKLSLFCETCDKLTCRDCQLSDHRDHKYKFAHEIATESRQALSTLVSEINYKRFLLSSATKVIDDRQQLIHDKKKDLIKEITAMAVKITNTVNTRGKQLIMRLNEVCDSKLKVLVEKKETLQLLSDNTDHCIDFMQNALEKGSDFAILSSKKSLVRHLQKLKCQRADIPNPEIPVRIQVQLNQVSDLQKVISQLGIIIVDGKPYPPTPSPNGTPQPQHPPRQPPSPNMAPPLRPGLPPGMPAGLSPNGPPVNFGPQNGPPLYSNAAQQQFNNLSMSRSFPGDGSVRFGGMPPVGMQRHGQPHVSSSTHPQNMDISLRGLLNNQAAQSPNAHMAFNGPPSYPGGPQGAPAPAHQPMGPQMRPHFMPGQQGFSQGGGPGGGPRDANFMNSNARFQSQYQRMANHAQQAAAAAAMAGAAGGGGGQIPSPGALQRPQMMSNPMQNSLGFHGSQAGFNTGPPQTSPQLGGGGMHSLAKWHIPQSAQQSNMCSQQGPLLPFANGRQTSENFKISLKSPNTLKNSTPPSLGGGGAGHQGHGNGSSSAQLNAAALGLGPAVSILSNVTSTNPKTPSPSTHENTKDFTEPIDKVRDDSINDLIATIAKLDSNGVQVLPEGRTKTTSPQVHSSTDLSNTQEVNNKNEQKDDPNEDWCAVCLDGGELMCCDKCPKVFHQNCHIPAISSLPDESESWQCLLCVNIKELTKTEGSEKSSSGELSALELRILQRICLELYCQYEQSLNFREPESPANTSYYEIVSSPMSLDVIRTRLDPSSPNHYKDIAGFVSDVRLIFSNTYLFYQEDTKTYSNAKYLENFFEEQLAKWLPQFEGTKPQGKRNTSNSPALLGVNATGSPSPIENGRKSCGSASLGDSDGACLPAKRARRSAHE

Target ORF information:

RefSeq Version	NM_001170194.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster bonus (bon), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170194.2

ORF Insert Sequence:

ATGGATATGG ATTTGGAGCA GCTAAAGAAC GACTTCCTGC CGCTTATCGC CGGGATCAAG 
CAGGAGCAGC TGGATGCCGT GCCCACGGAT GCCCTGCCCC AGATGAGCAC ACCAAACACG 
GCCAGCGGTG CGCCCACAAC CTCGTCATTG AGCTCCTCTT CGCTGAGTCT GAGCAACCCC 
TGCGACAGTG CCGAGAAAAG GTCGGAGTCA AACTCTTCGG CATCAGCCAA GTTTACCCTT 
TTCAAGTGCG TTTACTGCGC TCAACTCCTC GGATCCAACG ATCGCCCCAA GCTGTTAGAA 
TGCCTTCACG TGGCCTGTGC CCAGTGCGTG AGCACCAAGT TCTCAGAGCT GGACCGTTCC 
CTGCCACCAT TGATCCATTG TCCGGTATGC GACAATGCGT CGCAGCAGGA GTTCATCGTG 
GACAACCAGT TCCTCATTGA GCAGTGCACC GCCGGAGACA GTGGCGATGG GGTCGGACTT 
CTGGGTCTGA CTGGCGAAGG TCAGAAGAGT TCCGCTGCGG CGAGCATCCA GTGCAGCAGC 
TGCTCCGATG GTGCGGTGGC CACGTCGTGG TGCGTTGACT GCTCGGAGTA TATTTGCGAC 
AGTTGTGTGC AAGCTCACCA GCGCCTGAAG ATCACCAAGG ATCACACGAT CAAGCCCAAA 
GATGAGGCCA ACAACGAGCA GCTGGCCGGA GCTGCCGGGG TGGACAAGTT GCACATGTGC 
CAGTTGCATC CGCAGGAGAA GCTTTCGCTA TTTTGCGAGA CCTGCGACAA GCTCACTTGT 
CGGGATTGCC AGTTGAGCGA CCATCGGGAT CACAAATACA AGTTTGCGCA CGAGATTGCC 
ACGGAATCGC GACAGGCGCT GTCCACACTC GTTTCCGAAA TCAACTACAA ACGCTTCCTT 
CTCTCCTCGG CAACCAAGGT TATTGATGAC CGCCAACAGC TGATCCACGA CAAGAAGAAA 
GACCTCATCA AGGAGATCAC AGCCATGGCG GTGAAGATCA CCAATACGGT CAATACCCGA 
GGCAAGCAGC TAATCATGAG ATTGAACGAG GTGTGCGACA GTAAACTCAA GGTGCTGGTG 
GAAAAGAAGG AGACGCTGCA GTTGCTCTCC GACAACACGG ACCACTGCAT CGATTTTATG 
CAAAACGCTT TGGAGAAGGG CAGTGATTTC GCCATCCTCT CGAGCAAAAA GTCTCTGGTG 
CGTCACCTGC AGAAGCTGAA GTGCCAACGG GCGGATATTC CAAATCCGGA AATCCCAGTG 
CGCATTCAGG TGCAGCTTAA TCAGGTTTCC GATCTGCAGA AGGTAATCTC CCAGCTGGGC 
ATCATTATCG TCGATGGCAA ACCATATCCG CCCACACCGT CGCCCAACGG AACCCCTCAG 
CCGCAACACC CACCGCGCCA GCCTCCTAGC CCGAACATGG CTCCACCCCT GCGTCCTGGT 
CTTCCACCCG GAATGCCAGC TGGCCTCTCG CCGAACGGAC CGCCCGTCAA CTTCGGACCG 
CAGAACGGAC CGCCGCTCTA CAGCAATGCT GCCCAGCAGC AATTCAACAA TCTGTCCATG 
AGTCGCTCCT TTCCGGGTGA CGGGTCAGTT CGGTTCGGGG GAATGCCGCC AGTGGGCATG 
CAGCGACACG GACAACCGCA CGTGAGCTCC TCCACACATC CGCAGAATAT GGACATCAGC 
CTGCGGGGCC TACTGAACAA CCAGGCGGCG CAGAGCCCGA ATGCCCACAT GGCATTTAAT 
GGACCGCCCA GCTATCCGGG TGGGCCGCAA GGAGCACCGG CTCCGGCCCA CCAACCGATG 
GGCCCGCAGA TGCGTCCGCA TTTCATGCCC GGCCAGCAGG GTTTCTCGCA GGGCGGTGGA 
CCAGGAGGCG GTCCGCGGGA CGCCAACTTT ATGAACAGCA ACGCGCGCTT CCAGTCGCAG 
TATCAGCGTA TGGCCAACCA CGCTCAGCAG GCGGCGGCTG CGGCGGCCAT GGCCGGTGCG 
GCGGGGGGCG GAGGCGGCCA AATCCCTTCG CCGGGTGCAC TGCAGCGACC GCAGATGATG 
TCCAATCCCA TGCAGAATTC GTTGGGGTTT CATGGGAGCC AGGCTGGCTT TAATACCGGC 
CCACCACAGA CCTCCCCGCA GTTAGGCGGC GGAGGCATGC ACAGCCTGGC CAAGTGGCAC 
ATCCCGCAAT CCGCGCAACA GTCGAACATG TGTTCGCAAC AAGGCCCCCT TTTGCCTTTT 
GCGAATGGCC GCCAGACATC GGAAAATTTT AAAATCTCAC TTAAATCTCC CAACACGCTC 
AAAAATAGCA CCCCGCCCAG CCTGGGGGGC GGTGGTGCGG GTCACCAGGG CCACGGAAAC 
GGCTCCTCCA GCGCCCAACT GAACGCAGCT GCTCTGGGAT TGGGGCCAGC CGTTTCGATA 
CTCTCCAATG TCACCTCGAC GAATCCAAAG ACGCCCAGTC CCAGCACTCA TGAGAACACC 
AAGGACTTCA CCGAACCCAT TGACAAGGTG CGAGATGACT CAATCAACGA TCTAATTGCA 
ACCATAGCCA AACTGGACTC GAATGGGGTA CAGGTATTGC CGGAGGGTCG GACAAAGACC 
ACCTCGCCGC AGGTGCACAG CTCGACGGAT CTGTCCAACA CACAGGAAGT TAATAATAAA 
AACGAACAAA AAGATGATCC CAACGAGGAC TGGTGCGCCG TCTGTCTGGA TGGAGGCGAG 
CTGATGTGCT GCGACAAGTG TCCCAAAGTT TTCCATCAGA ACTGTCACAT CCCTGCGATC 
AGCTCGTTGC CGGACGAGAG CGAGAGCTGG CAGTGCCTAC TGTGCGTCAA CATCAAGGAG 
CTGACAAAGA CGGAGGGAAG TGAAAAGAGC TCCTCCGGCG AGCTGAGCGC CCTCGAACTC 
CGCATCTTGC AGCGCATCTG CCTAGAATTG TACTGCCAGT ACGAGCAGAG TCTCAACTTC 
CGGGAGCCGG AGTCGCCAGC CAATACATCC TACTACGAGA TTGTTTCCAG TCCCATGTCT 
CTAGATGTTA TTCGCACCCG CCTGGATCCA TCCAGTCCGA ATCACTACAA GGACATTGCA 
GGCTTCGTGT CCGACGTGCG TTTAATATTC TCCAACACAT ACCTCTTTTA CCAGGAGGAC 
ACGAAAACTT ACTCCAATGC CAAGTACTTG GAAAATTTCT TCGAGGAGCA GCTGGCCAAG 
TGGTTGCCGC AATTTGAGGG CACCAAGCCG CAGGGTAAGC GAAATACCTC AAACTCGCCG 
GCGCTGTTAG GTGTGAATGC AACTGGTTCG CCGTCGCCCA TCGAGAACGG ACGGAAGAGC 
TGCGGCTCGG CATCATTGGG TGACAGCGAT GGTGCCTGCT TGCCGGCCAA GAGGGCGAGA 
CGCTCGGCGC ACGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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bon cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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