mAcon1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mAcon1
Entrez Gene ID	44149
Full Name	Aconitase
Synonyms	ACON-1,AconM,BEST:GH10550,CG9244,Dmel\CG9244,FBgn0010100,M-Acon,acon,hsp60,i204,i59,l(2)07054,m-Acon,m-acon,mACON,mAc,mAcon
General protein information	Preferred Names Aconitase Names CG9244 gene product from transcript CG9244-RF ACONITASE Acon-PB Acon-PD Acon-PE Acon-PF CG9244-PB CG9244-PD CG9244-PE CG9244-PF aconitase m-aconitase mitochondrial aconitase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 39A7-39A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_079969.5	NP_524708.1	mitochondrial aconitase 1, isoform B
NM_001169559.2	NP_001163030.1	mitochondrial aconitase 1, isoform D
NM_001103716.2	NP_001097186.2	mitochondrial aconitase 1, isoform E
NM_001299190.1	NP_001286119.1	mitochondrial aconitase 1, isoform F

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aco2	NP_542364.1
Caenorhabditis elegans (roundworm)	aco-2	NP_741235.1
Saccharomyces cerevisiae (baker's yeast)	ACO1	NP_013407.1
Canis lupus familiaris (dog)	ACO2	XP_849166.1
Gallus gallus (chicken)	ACO2	NP_989519.1
	ACO2	XP_001105023.1
Drosophila melanogaster (fruit fly)	Acon	NP_001163030.1
Bos taurus (cattle)	ACO2	NP_776402.1
Rattus norvegicus (Norway rat)	Aco2	NP_077374.2
Danio rerio (zebrafish)	aco2	NP_944590.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007852	XP_317642.4
Eremothecium gossypii	AGOS_ADL032W	NP_984065.1
Schizosaccharomyces pombe (fission yeast)	SPAC24C9.06c	NP_594031.2
Magnaporthe oryzae (rice blast fungus)	MGG_03521	XP_003716398.1
Neurospora crassa	NCU02366	XP_959787.1
Xenopus tropicalis (tropical clawed frog)	aco2	NP_001011445.1
Homo sapiens (human)	ACO2	NP_001089.1
Kluyveromyces lactis	KLLA0C17314g	XP_452974.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RNA interference-generated Acon-knockdown flies exhibited a variety of phenotypes, such as reduced locomotor activity, a shortened lifespan, and increased cell death in the developing brain.
Title: Impaired energy metabolism in a Drosophila model of mitochondrial aconitase deficiency.

The following mAcon1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mAcon1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15191	NM_001169559.2 Latest version!	Drosophila melanogaster mitochondrial aconitase 1, transcript variant D (mAcon1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15191	NM_079969.5 Latest version!	Drosophila melanogaster mitochondrial aconitase 1, transcript variant B (mAcon1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15191	NM_001299190.1 Latest version!	Drosophila melanogaster mitochondrial aconitase 1, transcript variant F (mAcon1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa15191	NM_001103716.2 Latest version!	Drosophila melanogaster mitochondrial aconitase 1, transcript variant E (mAcon1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mAcon1 ( NM_079969.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524708.1 Drosophila melanogaster mitochondrial aconitase 1, transcript variant B (mAcon1), mRNA.

Price & Availability↑

CloneID	OFa15191
Clone ID Related Accession (Same CDS sequence)	NM_079969.5 , NM_001169559.2 , NM_001103716.2 , NM_001299190.1
Accession Version	NM_079969.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial aconitase 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_079969.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524708.1
CDS	122..2485
Translation	MAARLMNAQAQVCRLGKHVASEATVVRQFHASCYTASKVALSKFDSDVYLPYEKLNKRLEVVRGRLNRPLTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVGLIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRLDFNPLTDELTGADGKKFKLKAPFGDELPAKGFDPGQDTYTAPPPSGENVKVAVDPKSTRLQLLEPFDKWNGQDLTDLTVLIKVKGKCTTDHISAAGPWLKYRGHLDNISNNMFIGATNYENNEMNNIKNQRNGSWGGVPDVARDYKANGIKWVAVGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGLLPLTFANPADYDKIQPTSKISLLNLKSLAPGKPVDAEIKNGDKVERIKLNHTLNDLQIGWFKAGSALNRMKELAQ

Target ORF information:

RefSeq Version	NM_079969.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial aconitase 1, transcript variant B (mAcon1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079969.5

ORF Insert Sequence:

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mAcon1 ( NM_001169559.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163030.1 Drosophila melanogaster mitochondrial aconitase 1, transcript variant D (mAcon1), mRNA.

Price & Availability↑

CloneID	OFa15191
Clone ID Related Accession (Same CDS sequence)	NM_079969.5 , NM_001169559.2 , NM_001103716.2 , NM_001299190.1
Accession Version	NM_001169559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial aconitase 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169559.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163030.1
CDS	122..2485
Translation	MAARLMNAQAQVCRLGKHVASEATVVRQFHASCYTASKVALSKFDSDVYLPYEKLNKRLEVVRGRLNRPLTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVGLIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRLDFNPLTDELTGADGKKFKLKAPFGDELPAKGFDPGQDTYTAPPPSGENVKVAVDPKSTRLQLLEPFDKWNGQDLTDLTVLIKVKGKCTTDHISAAGPWLKYRGHLDNISNNMFIGATNYENNEMNNIKNQRNGSWGGVPDVARDYKANGIKWVAVGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGLLPLTFANPADYDKIQPTSKISLLNLKSLAPGKPVDAEIKNGDKVERIKLNHTLNDLQIGWFKAGSALNRMKELAQ

Target ORF information:

RefSeq Version	NM_001169559.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial aconitase 1, transcript variant D (mAcon1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169559.2

ORF Insert Sequence:

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mAcon1 ( NM_001103716.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097186.2 Drosophila melanogaster mitochondrial aconitase 1, transcript variant E (mAcon1), mRNA.

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CloneID	OFa15191
Clone ID Related Accession (Same CDS sequence)	NM_079969.5 , NM_001169559.2 , NM_001103716.2 , NM_001299190.1
Accession Version	NM_001103716.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial aconitase 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001103716.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097186.2
CDS	122..2485
Translation	MAARLMNAQAQVCRLGKHVASEATVVRQFHASCYTASKVALSKFDSDVYLPYEKLNKRLEVVRGRLNRPLTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVGLIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRLDFNPLTDELTGADGKKFKLKAPFGDELPAKGFDPGQDTYTAPPPSGENVKVAVDPKSTRLQLLEPFDKWNGQDLTDLTVLIKVKGKCTTDHISAAGPWLKYRGHLDNISNNMFIGATNYENNEMNNIKNQRNGSWGGVPDVARDYKANGIKWVAVGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGLLPLTFANPADYDKIQPTSKISLLNLKSLAPGKPVDAEIKNGDKVERIKLNHTLNDLQIGWFKAGSALNRMKELAQ

Target ORF information:

RefSeq Version	NM_001103716.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial aconitase 1, transcript variant E (mAcon1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103716.2

ORF Insert Sequence:

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mAcon1 ( NM_001299190.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286119.1 Drosophila melanogaster mitochondrial aconitase 1, transcript variant F (mAcon1), mRNA.

Price & Availability↑

CloneID	OFa15191
Clone ID Related Accession (Same CDS sequence)	NM_079969.5 , NM_001169559.2 , NM_001103716.2 , NM_001299190.1
Accession Version	NM_001299190.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial aconitase 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286119.1
CDS	203..2566
Translation	MAARLMNAQAQVCRLGKHVASEATVVRQFHASCYTASKVALSKFDSDVYLPYEKLNKRLEVVRGRLNRPLTLSEKVLYSHLDDPANQDIVRGTSYLRLRPDRVAMQDATAQMALLQFISSGLKKVAVPSTVHCDHLIEAQIGGPKDLARAKDLNKEVYDFLASTCAKYGLGFWKPGSGIIHQIILENYAFPGLLMIGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVNLTGKISGWTSPKDVILKVADILTVKGGTGAIIEYHGKGVDSISCTGMATICNMGAEIGATTSLFPFNQRMADYLKSTGRAGIASEAQKYQAKILSADKNCEYDELIEINLDTLEPHVNGPFTPDLGHPISKLGENSKKNGYPMDIRVGLIGSCTNSSYEDMGRCASIAKDAMSHGLKSKIPFNVTPGSEQIRATIERDGISEVFDKFGGTVLANACGPCIGQWDRKDVKKGDKNTIVTSYNRNFTGRNDANPATHCFVTSPELVTALSIAGRLDFNPLTDELTGADGKKFKLKAPFGDELPAKGFDPGQDTYTAPPPSGENVKVAVDPKSTRLQLLEPFDKWNGQDLTDLTVLIKVKGKCTTDHISAAGPWLKYRGHLDNISNNMFIGATNYENNEMNNIKNQRNGSWGGVPDVARDYKANGIKWVAVGDENYGEGSSREHAALEPRHLGGRAIIVKSFARIHETNLKKQGLLPLTFANPADYDKIQPTSKISLLNLKSLAPGKPVDAEIKNGDKVERIKLNHTLNDLQIGWFKAGSALNRMKELAQ

Target ORF information:

RefSeq Version	NM_001299190.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial aconitase 1, transcript variant F (mAcon1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299190.1

ORF Insert Sequence:

ATGGCTGCGA GATTGATGAA CGCCCAGGCA CAGGTGTGTC GACTGGGCAA GCATGTTGCG 
TCCGAAGCCA CAGTGGTGCG TCAGTTCCAC GCCTCCTGCT ACACCGCCTC CAAAGTGGCT 
CTGTCCAAGT TCGACTCGGA CGTCTACCTG CCGTACGAGA AGCTGAACAA GCGCCTGGAA 
GTGGTGCGCG GTCGCTTGAA CCGCCCACTG ACTCTCTCCG AGAAGGTGCT GTACTCGCAT 
CTGGACGATC CCGCAAACCA GGACATCGTG CGCGGTACTT CGTACCTGCG TCTTCGCCCC 
GATCGTGTGG CTATGCAGGA TGCCACTGCA CAGATGGCTC TGCTTCAGTT TATCTCCTCC 
GGACTGAAAA AGGTGGCTGT GCCCTCCACT GTTCACTGTG ATCATTTAAT CGAGGCCCAG 
ATTGGTGGAC CCAAGGATTT AGCCCGTGCC AAGGACCTGA ACAAGGAAGT GTACGATTTC 
TTGGCCAGCA CCTGTGCAAA GTACGGATTG GGCTTCTGGA AGCCAGGCAG CGGAATCATC 
CATCAGATCA TTCTGGAGAA CTACGCTTTC CCCGGCCTGC TGATGATTGG TACTGACTCA 
CACACCCCCA ACGGTGGTGG CTTGGGTGGC CTGTGCATTG GCGTTGGTGG TGCTGATGCC 
GTCGATGTGA TGGCCGACAT CCCCTGGGAG CTGAAGTGCC CCAAGGTGAT TGGTGTCAAT 
TTGACTGGCA AGATCAGCGG CTGGACCTCG CCCAAGGATG TTATCCTGAA GGTTGCCGAT 
ATCCTGACCG TCAAGGGAGG CACCGGTGCC ATCATTGAGT ATCACGGCAA GGGTGTTGAC 
TCTATCTCTT GTACAGGCAT GGCCACCATC TGCAACATGG GTGCTGAGAT CGGAGCTACC 
ACCTCTCTGT TCCCCTTCAA CCAGCGCATG GCTGATTACC TGAAATCGAC AGGCCGTGCT 
GGCATTGCCT CCGAAGCCCA GAAGTACCAG GCCAAGATTC TGTCCGCTGA CAAGAACTGC 
GAGTACGATG AGCTGATTGA AATCAACCTA GACACCCTGG AGCCCCATGT TAACGGACCA 
TTCACTCCCG ATTTGGGCCA TCCCATCAGC AAGCTAGGCG AGAACTCGAA GAAGAACGGG 
TACCCTATGG ACATCCGTGT AGGTCTTATT GGCTCTTGCA CCAACTCTTC GTATGAGGAC 
ATGGGTCGCT GCGCCAGCAT CGCCAAGGAT GCCATGAGCC ATGGCCTCAA GTCGAAGATT 
CCTTTCAATG TGACTCCCGG ATCAGAGCAG ATCCGTGCCA CCATCGAGCG CGACGGCATC 
TCTGAGGTGT TCGACAAGTT CGGCGGTACC GTTCTGGCCA ACGCTTGCGG TCCCTGCATT 
GGACAGTGGG ATCGTAAGGA CGTTAAGAAG GGCGACAAGA ACACCATTGT CACCTCCTAC 
AACCGAAACT TTACTGGCAG GAACGACGCT AACCCGGCCA CCCATTGCTT TGTCACTAGT 
CCCGAGTTGG TCACCGCTCT GTCCATCGCC GGTCGTCTGG ACTTCAACCC GCTGACCGAC 
GAGCTTACTG GTGCTGATGG CAAGAAGTTC AAGCTCAAGG CTCCCTTCGG CGACGAGCTG 
CCCGCCAAGG GTTTCGATCC CGGCCAGGAC ACCTACACTG CTCCCCCACC CAGCGGCGAA 
AATGTCAAGG TGGCTGTCGA CCCCAAGTCG ACGCGTCTGC AGCTGCTGGA GCCTTTTGAC 
AAGTGGAACG GCCAGGATCT GACCGATCTG ACTGTGCTGA TCAAGGTTAA GGGAAAGTGC 
ACCACTGACC ACATCTCCGC TGCCGGACCC TGGCTGAAGT ACCGTGGCCA TTTGGACAAC 
ATCTCCAACA ACATGTTTAT CGGAGCCACC AACTACGAAA ACAACGAGAT GAACAACATC 
AAGAACCAAC GCAACGGTAG CTGGGGAGGA GTTCCCGACG TAGCCCGCGA CTACAAGGCC 
AACGGCATCA AGTGGGTGGC CGTCGGCGAT GAGAACTACG GCGAGGGTTC GTCCCGCGAG 
CATGCCGCTT TGGAGCCCCG TCACCTTGGT GGTCGTGCCA TTATCGTCAA GTCCTTCGCC 
CGTATCCACG AGACCAACCT GAAGAAACAG GGTCTTCTGC CTCTCACCTT CGCCAATCCC 
GCTGATTACG ATAAGATCCA GCCCACGAGC AAAATCTCCC TGCTCAACCT GAAATCCCTT 
GCGCCTGGAA AGCCCGTAGA TGCCGAAATC AAGAATGGCG ACAAGGTCGA GAGGATCAAG 
CTTAACCACA CCCTGAACGA CCTACAGATC GGCTGGTTCA AGGCCGGCAG CGCTCTCAAC 
CGCATGAAGG AGCTGGCCCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mAcon1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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