hbs cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hbs
Entrez Gene ID	44129
Full Name	hibris
Synonyms	CG7449,CT22841,Dmel\CG7449,Hbs,icon
General protein information	Preferred Names hibris Names CG7449 gene product from transcript CG7449-RC CG7449-PA CG7449-PB CG7449-PC hbs-PA hbs-PB hbs-PC inappropriate contact inappropriate contacts
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 51D8-51D8

mRNA and Protein(s)

mRNA	Protein	Name
NM_079963.4	NP_524702.3	hibris, isoform A
NM_001299510.1	NP_001286439.1	hibris, isoform C
NM_176175.2	NP_788355.1	hibris, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	hbs	NP_524702.3
Caenorhabditis elegans (roundworm)	syg-2	NP_510435.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the roles of Rst, Hbs, Kirre and SNS in the spacing of bristles of the anterior wing margin, are explored.
Title: The Cell Adhesion Molecules Roughest, Hibris, Kin of Irre and Sticks and Stones Are Required for Long Range Spacing of the Drosophila Wing Disc Sensory Sensilla.

Cindr recapitulates both proposed functions of its mammalian orthologs Cd2ap and Cin85: targeting the IgCAMs Rst and Hbs for endocytosis and stabilizing these heterophilic IgCAM complexes.
Title: Interactions between Drosophila IgCAM adhesion receptors and cindr, the Cd2ap/Cin85 ortholog.

Consistent with its role in Psn processing, Hbs genetically interacts with the Drosophila beta-amyloid protein precursor-like (Appl) protein.
Title: Hibris, a Drosophila nephrin homolog, is required for presenilin-mediated Notch and APP-like cleavages.

identified two cell-adhesion molecules: Hibris, which is expressed in post-mitotic lamina precursor cells; and Roughest, which is expressed on retinal axons.
Title: Recognition of pre- and postsynaptic neurons via nephrin/NEPH1 homologs is a basis for the formation of the Drosophila retinotopic map.

The work indicates hbs contributes to the remodeling of cell contacts and cell shapes throughout development.
Title: Dynamic cell shapes and contacts in the developing Drosophila retina are regulated by the Ig cell adhesion protein hibris.

The following hbs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hbs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11328	NM_176175.2 Latest version!	Drosophila melanogaster hibris (hbs), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa11328	NM_001299510.1 Latest version!	Drosophila melanogaster hibris (hbs), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa11329	NM_079963.4 Latest version!	Drosophila melanogaster hibris (hbs), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hbs ( NM_001299510.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286439.1 Drosophila melanogaster hibris (hbs), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa11328
Clone ID Related Accession (Same CDS sequence)	NM_001299510.1 , NM_176175.2
Accession Version	NM_001299510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hibris, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTTTT GGATAACTCT GACATTTGCC GTGCACTTGG CTACGACCAT CTGCCACGTA 
ATGGCCGCAG TTACTGCTCC AACTCCGGCT GCTCAGGTGG CAGCCCCACC TGTGCAAAAG 
TTTCACCTGA CGCCCCACGA TCTGCAGATA CTCGAGGGCA CCGACACCCT CCTGCGCTGC 
GAGGTATCGA ACCGGGCGGG CAAAGTCCAG TGGACCAAGG ATGGCTTTGC GCTGGGATTC 
TCGGCCGTAA TTCCCGGATT CCCACGCTAT TCTGTGCTGG TGGATGCCAA ACAGAATGCC 
TACAATCTGC AGATCAAGAA CGCCACGCTG GAGGACGATG CCGAATACCA ATGCCAGGTG 
GGTCCTGCTG CTGGAAATCC CGCCATTCGT GCCAATGCCA AGTTGAGCAT AGTGGCTGCT 
CCGAGTAGCA TCGTTATCGA GGGCTATGCC CGGAATGCCC GAGTGGAGGT GGAGGAGCGC 
CAGAATCTTA CGCTGCACTG TATCGCCGAG AACGCCAATC CGGCGGCGGA GATTGTCTGG 
TTCCAGGGCG AAGTTCCAGT CTCCACGGCC CCCATCGTGA CGGTGAATCA AACTGCCCCG 
AAGCGATTCA CAACTAGCTC CACTTTGCAC CTGCAGCCGC GCGCCGAAGA CGATTACAAG 
GAGTTCTCCT GCGAGGCGCG TCACAAGGCT CTGCCCCCCG ATGTACCGAT GCGCGCCCAG 
GTGCAGCTCT CCGTACTCTA TCCGCCCGGC GCTCCGTTCT TTGAGGGCTA CAGCCAGGGT 
GAGACCCTGC ATCGCGGCCA GGAAGTTCAA ATCGCCTGTC GAAGCCGCGG CGGCAATCCC 
CCGGCCCAAT TGACTTGGTA CCGAAATGGC GTGGCCATCA GTTCCCCACA ACGCACCTCG 
GGCCGTCTGT CGGAGAACGT TTACAAGTTC ACCGCCGCCG CCGAGGACAA TGGGGCTAAT 
TTGGTGTGCG AGGCCAAAAA TCTGCTGGCG ACAACTCCTC TGCGCGCCGA ACTCAATTTG 
ACTGTCCTGT ATGCTCCCAA GGATGTTTAT CTGAGCGGCG CCAATCAGGC CAAGGTCGGC 
GATTCCGTGC AACTAAGCTG CGTGACAGCG CCTTCGAATC CCCAGGCCCG AATTAGCTGG 
TCCATAAATG GCAGACCGCT GGACAATAGC ACCTATAAGA CGACCAGTAG CTCCGACGGT 
GGCTGGGTCA GTAGTTCCAA CATCAGCTTG ACTATAGATT CGCAGAGCAG GACTTTCATC 
GCCGTTTGCC ACGCCCTCAA CACGGAACTC ACTCAGAATG TGGTGGGATC ACACACCGTG 
AATGTGCTTT ATCCCCCTTC GCCACCCTTG TTGACGGGCT ATAATGATGG TGATATCCTT 
ATCTCGGGAT CGATTTTGAA ACTTCAGTGC AGTTCTGCTG GCGGAAATCC TCCACCGACA 
TTGCAGTGGT ACAAAAACGA TAAGATCATT AATGCTCCCT CCAAACTGGT GGACAGTAAA 
ATCACCTCTG AGCTCTCACT TTTGGTAAAC GCCTCCGATA ACAATGCCAT CTATAAGTGC 
AAGGTTCAAA ATGCAGCTAT TGACATACCA CTATTTGCCA CCAAAACTTT GGGAGTTCAT 
TTTCCTCCCG AAACGGTTAA GATCAGTGTC GTGCCCAAGA ATCTAGTGCC TGGCATTCGA 
GCCAAGCTGA TCTGTGACTC CAGCTCTAGT AATCCACCGG CGAAGATAAG CTGGTGGAAA 
GATGGCATTC CCGTCGAAGG TCTTAACTTG GCCAATAGGC CCGGTCTCTG GGGCGGTTCG 
GTGTCCACGC TTGAGATGTA CGTTAACATA ACCCAGGATC TGGATGGCAT TGTCTATACC 
TGTCAGAGTC ACAACGAGGT TCTGCAGCGC AGTGTTCACG AAACAATTAG CTTGGATATA 
CTTTATCCCC CCAAATTCGA GACGCCACAG AGCACCACCT TCGTGGGCGT TGAGGGAGCG 
CCGTTCCATG TGGAGCTGCT GGCCAGCGGA AACCCCATGG TAATTACCTA TACCTGGACC 
AAGGACGGCC TGCCCATTAG CAGCAATAGT CTGAGCGGGC AGCGTTTGAT CTCCGACGGA 
CCTCGTCTCA ATATCAGTCG TCTGAGTCGC AATGATGCGG GTGTCTACAT ATGCGAGGCG 
CTCAATAGCC AGGGCACCGC GCTGCTGGAG ATTCAAGTGG CCGTGGAATA TGCCCCCACA 
ATTACGGCGG TTAGCGAGGG TCGCAGTTTT GTGGCCGGCG AACCGGCGGT CTTGGCCTGC 
CACATTCAGG CCCGCCCGCT AGAGGCCGCT CATGTGCGCT GGTCCCGCGA CGGTTATGAT 
CTGGCCACGC GCACCATATC GAGTTTTGAA AATGGAACCG CCCTGCTGCA GATCGCCAGT 
GTGGAGCGCA GTGATATTGG CAACTTTACC TGCATTGTGG ACAACCAAAG AGGAGCTCCC 
GCCGCGCAAA ACGTACTTCT GGTGGTGCAA ACTGCCCCTG AAATTGACCA CTCACCCGGT 
TTCACCCGCT ATGCTGCCCG TTTGGGAGTT CGTGCCCAGC TCATTTGCAG ATCTCTGGCC 
TCGCCGCAGC CCAGTTTCAT CTGGCGTCGT CATGGCAAGG ATCTCAAGAT GCAACGGCGC 
AACAAATTCA AAAGCGTGGA ACGCCAGGTG GATGCCCTGA ACTTTGAGTC GGCTCTGCTA 
ATCGAAAACA CTTCGCCCGA TGATTATGGT CAGTACGAGT GTGTGGTGCG AAATTCGCTG 
GGACAGGCGA GTACCACCCT GGAGTTTAGC AAACCCTCTC GCCCGGATGC TCCGCTGCAG 
CTGAGGGTGG GCAATGTGAG TGATACGGGT GTGGATCTGA ACTGGACTCC CGGCTTCGAT 
GGTGGCATGC AGACATATTT CCGTCTGCGG CTCAAACAGC ACGGCGAGGA TAAGTACAAG 
TACGTGGACG CCAAGCCCGG ACATCAGAAT ATATCCCTTG ATGGCCTGAA GCCAGGAGCA 
ACCTACTACT TCTCCGTGAT GGCCGCCAAT GAAGCGGGAG GCAGTAAATT CATGCCCGAT 
ATCAAGTTGA CCCTCAGCAA GGGATCGCAG CCCCATTCGG CGGAGTACAC CGAAAAAGAC 
GAACTGCCCA ATGTGATGAT CATTGGAATC ACCTCAGCAG CCATGGTTTT GTTGGTTCTG 
AACGCTGCCC TGGTGGCCTG GTTCGTGATC CGCCGGCAGA ACAAATCTCA GAGCGAAGCG 
GAGCCGAGCA ACGATGATGT GTACTCCAAG GATGACAGTC AGTCTGTCTA CAAGCTGCCC 
ATCACTGCTT TGCAGGCGGA TGTTCAGAAG AAAGCAGCCG CCTCCACATA TCTCGTGGAG 
AACGTGGACA TTATCCAGTC GACGGCGTAT CCGCCGAAAT ATCAGGAGAG CTCCATGTGC 
ACCCCGCCCT ATCCGCTCTG CAATCCGGAC TTCACCCGGA CCCTGCCGAA TCCCAAGCGG 
CACAGTCAGC GGAACAGTGC CACGGGCATG ATCGAGGGCA TGCAGATGCG CTCCAAGGAC 
GATCATATGC TGATCTCCAA CGGACTGTAC ATACCATCAC CGTCGCCGGC CTCCTCCCTG 
GTGATCAAGG GTAGCTACAT ATCCTCACCA TCGCCAGCGC CGCCAGCAGA TGGATCATAC 
TTTAATATGA GCGACAAATA TATGTCCTAT CCGCCGGTGA CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286439.1
CDS	396..4103
Translation	MQFWITLTFAVHLATTICHVMAAVTAPTPAAQVAAPPVQKFHLTPHDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSRGGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGANQAKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAHVRWSRDGYDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPDIKLTLSKGSQPHSAEYTEKDELPNVMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAEPSNDDVYSKDDSQSVYKLPITALQADVQKKAAASTYLVENVDIIQSTAYPPKYQESSMCTPPYPLCNPDFTRTLPNPKRHSQRNSATGMIEGMQMRSKDDHMLISNGLYIPSPSPASSLVIKGSYISSPSPAPPADGSYFNMSDKYMSYPPVTY

Target ORF information:

RefSeq Version	NM_001299510.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hibris (hbs), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299510.1

ORF Insert Sequence:

ATGCAGTTTT GGATAACTCT GACATTTGCC GTGCACTTGG CTACGACCAT CTGCCACGTA 
ATGGCCGCAG TTACTGCTCC AACTCCGGCT GCTCAGGTGG CAGCCCCACC TGTGCAAAAG 
TTTCACCTGA CGCCCCACGA TCTGCAGATA CTCGAGGGCA CCGACACCCT CCTGCGCTGC 
GAGGTATCGA ACCGGGCGGG CAAAGTCCAG TGGACCAAGG ATGGCTTTGC GCTGGGATTC 
TCGGCCGTAA TTCCCGGATT CCCACGCTAT TCTGTGCTGG TGGATGCCAA ACAGAATGCC 
TACAATCTGC AGATCAAGAA CGCCACGCTG GAGGACGATG CCGAATACCA ATGCCAGGTG 
GGTCCTGCTG CTGGAAATCC CGCCATTCGT GCCAATGCCA AGTTGAGCAT AGTGGCTGCT 
CCGAGTAGCA TCGTTATCGA GGGCTATGCC CGGAATGCCC GAGTGGAGGT GGAGGAGCGC 
CAGAATCTTA CGCTGCACTG TATCGCCGAG AACGCCAATC CGGCGGCGGA GATTGTCTGG 
TTCCAGGGCG AAGTTCCAGT CTCCACGGCC CCCATCGTGA CGGTGAATCA AACTGCCCCG 
AAGCGATTCA CAACTAGCTC CACTTTGCAC CTGCAGCCGC GCGCCGAAGA CGATTACAAG 
GAGTTCTCCT GCGAGGCGCG TCACAAGGCT CTGCCCCCCG ATGTACCGAT GCGCGCCCAG 
GTGCAGCTCT CCGTACTCTA TCCGCCCGGC GCTCCGTTCT TTGAGGGCTA CAGCCAGGGT 
GAGACCCTGC ATCGCGGCCA GGAAGTTCAA ATCGCCTGTC GAAGCCGCGG CGGCAATCCC 
CCGGCCCAAT TGACTTGGTA CCGAAATGGC GTGGCCATCA GTTCCCCACA ACGCACCTCG 
GGCCGTCTGT CGGAGAACGT TTACAAGTTC ACCGCCGCCG CCGAGGACAA TGGGGCTAAT 
TTGGTGTGCG AGGCCAAAAA TCTGCTGGCG ACAACTCCTC TGCGCGCCGA ACTCAATTTG 
ACTGTCCTGT ATGCTCCCAA GGATGTTTAT CTGAGCGGCG CCAATCAGGC CAAGGTCGGC 
GATTCCGTGC AACTAAGCTG CGTGACAGCG CCTTCGAATC CCCAGGCCCG AATTAGCTGG 
TCCATAAATG GCAGACCGCT GGACAATAGC ACCTATAAGA CGACCAGTAG CTCCGACGGT 
GGCTGGGTCA GTAGTTCCAA CATCAGCTTG ACTATAGATT CGCAGAGCAG GACTTTCATC 
GCCGTTTGCC ACGCCCTCAA CACGGAACTC ACTCAGAATG TGGTGGGATC ACACACCGTG 
AATGTGCTTT ATCCCCCTTC GCCACCCTTG TTGACGGGCT ATAATGATGG TGATATCCTT 
ATCTCGGGAT CGATTTTGAA ACTTCAGTGC AGTTCTGCTG GCGGAAATCC TCCACCGACA 
TTGCAGTGGT ACAAAAACGA TAAGATCATT AATGCTCCCT CCAAACTGGT GGACAGTAAA 
ATCACCTCTG AGCTCTCACT TTTGGTAAAC GCCTCCGATA ACAATGCCAT CTATAAGTGC 
AAGGTTCAAA ATGCAGCTAT TGACATACCA CTATTTGCCA CCAAAACTTT GGGAGTTCAT 
TTTCCTCCCG AAACGGTTAA GATCAGTGTC GTGCCCAAGA ATCTAGTGCC TGGCATTCGA 
GCCAAGCTGA TCTGTGACTC CAGCTCTAGT AATCCACCGG CGAAGATAAG CTGGTGGAAA 
GATGGCATTC CCGTCGAAGG TCTTAACTTG GCCAATAGGC CCGGTCTCTG GGGCGGTTCG 
GTGTCCACGC TTGAGATGTA CGTTAACATA ACCCAGGATC TGGATGGCAT TGTCTATACC 
TGTCAGAGTC ACAACGAGGT TCTGCAGCGC AGTGTTCACG AAACAATTAG CTTGGATATA 
CTTTATCCCC CCAAATTCGA GACGCCACAG AGCACCACCT TCGTGGGCGT TGAGGGAGCG 
CCGTTCCATG TGGAGCTGCT GGCCAGCGGA AACCCCATGG TAATTACCTA TACCTGGACC 
AAGGACGGCC TGCCCATTAG CAGCAATAGT CTGAGCGGGC AGCGTTTGAT CTCCGACGGA 
CCTCGTCTCA ATATCAGTCG TCTGAGTCGC AATGATGCGG GTGTCTACAT ATGCGAGGCG 
CTCAATAGCC AGGGCACCGC GCTGCTGGAG ATTCAAGTGG CCGTGGAATA TGCCCCCACA 
ATTACGGCGG TTAGCGAGGG TCGCAGTTTT GTGGCCGGCG AACCGGCGGT CTTGGCCTGC 
CACATTCAGG CCCGCCCGCT AGAGGCCGCT CATGTGCGCT GGTCCCGCGA CGGTTATGAT 
CTGGCCACGC GCACCATATC GAGTTTTGAA AATGGAACCG CCCTGCTGCA GATCGCCAGT 
GTGGAGCGCA GTGATATTGG CAACTTTACC TGCATTGTGG ACAACCAAAG AGGAGCTCCC 
GCCGCGCAAA ACGTACTTCT GGTGGTGCAA ACTGCCCCTG AAATTGACCA CTCACCCGGT 
TTCACCCGCT ATGCTGCCCG TTTGGGAGTT CGTGCCCAGC TCATTTGCAG ATCTCTGGCC 
TCGCCGCAGC CCAGTTTCAT CTGGCGTCGT CATGGCAAGG ATCTCAAGAT GCAACGGCGC 
AACAAATTCA AAAGCGTGGA ACGCCAGGTG GATGCCCTGA ACTTTGAGTC GGCTCTGCTA 
ATCGAAAACA CTTCGCCCGA TGATTATGGT CAGTACGAGT GTGTGGTGCG AAATTCGCTG 
GGACAGGCGA GTACCACCCT GGAGTTTAGC AAACCCTCTC GCCCGGATGC TCCGCTGCAG 
CTGAGGGTGG GCAATGTGAG TGATACGGGT GTGGATCTGA ACTGGACTCC CGGCTTCGAT 
GGTGGCATGC AGACATATTT CCGTCTGCGG CTCAAACAGC ACGGCGAGGA TAAGTACAAG 
TACGTGGACG CCAAGCCCGG ACATCAGAAT ATATCCCTTG ATGGCCTGAA GCCAGGAGCA 
ACCTACTACT TCTCCGTGAT GGCCGCCAAT GAAGCGGGAG GCAGTAAATT CATGCCCGAT 
ATCAAGTTGA CCCTCAGCAA GGGATCGCAG CCCCATTCGG CGGAGTACAC CGAAAAAGAC 
GAACTGCCCA ATGTGATGAT CATTGGAATC ACCTCAGCAG CCATGGTTTT GTTGGTTCTG 
AACGCTGCCC TGGTGGCCTG GTTCGTGATC CGCCGGCAGA ACAAATCTCA GAGCGAAGCG 
GAGCCGAGCA ACGATGATGT GTACTCCAAG GATGACAGTC AGTCTGTCTA CAAGCTGCCC 
ATCACTGCTT TGCAGGCGGA TGTTCAGAAG AAAGCAGCCG CCTCCACATA TCTCGTGGAG 
AACGTGGACA TTATCCAGTC GACGGCGTAT CCGCCGAAAT ATCAGGAGAG CTCCATGTGC 
ACCCCGCCCT ATCCGCTCTG CAATCCGGAC TTCACCCGGA CCCTGCCGAA TCCCAAGCGG 
CACAGTCAGC GGAACAGTGC CACGGGCATG ATCGAGGGCA TGCAGATGCG CTCCAAGGAC 
GATCATATGC TGATCTCCAA CGGACTGTAC ATACCATCAC CGTCGCCGGC CTCCTCCCTG 
GTGATCAAGG GTAGCTACAT ATCCTCACCA TCGCCAGCGC CGCCAGCAGA TGGATCATAC 
TTTAATATGA GCGACAAATA TATGTCCTAT CCGCCGGTGA CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hbs ( NM_176175.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788355.1 Drosophila melanogaster hibris (hbs), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11328
Clone ID Related Accession (Same CDS sequence)	NM_001299510.1 , NM_176175.2
Accession Version	NM_176175.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hibris, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_176175.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTTTT GGATAACTCT GACATTTGCC GTGCACTTGG CTACGACCAT CTGCCACGTA 
ATGGCCGCAG TTACTGCTCC AACTCCGGCT GCTCAGGTGG CAGCCCCACC TGTGCAAAAG 
TTTCACCTGA CGCCCCACGA TCTGCAGATA CTCGAGGGCA CCGACACCCT CCTGCGCTGC 
GAGGTATCGA ACCGGGCGGG CAAAGTCCAG TGGACCAAGG ATGGCTTTGC GCTGGGATTC 
TCGGCCGTAA TTCCCGGATT CCCACGCTAT TCTGTGCTGG TGGATGCCAA ACAGAATGCC 
TACAATCTGC AGATCAAGAA CGCCACGCTG GAGGACGATG CCGAATACCA ATGCCAGGTG 
GGTCCTGCTG CTGGAAATCC CGCCATTCGT GCCAATGCCA AGTTGAGCAT AGTGGCTGCT 
CCGAGTAGCA TCGTTATCGA GGGCTATGCC CGGAATGCCC GAGTGGAGGT GGAGGAGCGC 
CAGAATCTTA CGCTGCACTG TATCGCCGAG AACGCCAATC CGGCGGCGGA GATTGTCTGG 
TTCCAGGGCG AAGTTCCAGT CTCCACGGCC CCCATCGTGA CGGTGAATCA AACTGCCCCG 
AAGCGATTCA CAACTAGCTC CACTTTGCAC CTGCAGCCGC GCGCCGAAGA CGATTACAAG 
GAGTTCTCCT GCGAGGCGCG TCACAAGGCT CTGCCCCCCG ATGTACCGAT GCGCGCCCAG 
GTGCAGCTCT CCGTACTCTA TCCGCCCGGC GCTCCGTTCT TTGAGGGCTA CAGCCAGGGT 
GAGACCCTGC ATCGCGGCCA GGAAGTTCAA ATCGCCTGTC GAAGCCGCGG CGGCAATCCC 
CCGGCCCAAT TGACTTGGTA CCGAAATGGC GTGGCCATCA GTTCCCCACA ACGCACCTCG 
GGCCGTCTGT CGGAGAACGT TTACAAGTTC ACCGCCGCCG CCGAGGACAA TGGGGCTAAT 
TTGGTGTGCG AGGCCAAAAA TCTGCTGGCG ACAACTCCTC TGCGCGCCGA ACTCAATTTG 
ACTGTCCTGT ATGCTCCCAA GGATGTTTAT CTGAGCGGCG CCAATCAGGC CAAGGTCGGC 
GATTCCGTGC AACTAAGCTG CGTGACAGCG CCTTCGAATC CCCAGGCCCG AATTAGCTGG 
TCCATAAATG GCAGACCGCT GGACAATAGC ACCTATAAGA CGACCAGTAG CTCCGACGGT 
GGCTGGGTCA GTAGTTCCAA CATCAGCTTG ACTATAGATT CGCAGAGCAG GACTTTCATC 
GCCGTTTGCC ACGCCCTCAA CACGGAACTC ACTCAGAATG TGGTGGGATC ACACACCGTG 
AATGTGCTTT ATCCCCCTTC GCCACCCTTG TTGACGGGCT ATAATGATGG TGATATCCTT 
ATCTCGGGAT CGATTTTGAA ACTTCAGTGC AGTTCTGCTG GCGGAAATCC TCCACCGACA 
TTGCAGTGGT ACAAAAACGA TAAGATCATT AATGCTCCCT CCAAACTGGT GGACAGTAAA 
ATCACCTCTG AGCTCTCACT TTTGGTAAAC GCCTCCGATA ACAATGCCAT CTATAAGTGC 
AAGGTTCAAA ATGCAGCTAT TGACATACCA CTATTTGCCA CCAAAACTTT GGGAGTTCAT 
TTTCCTCCCG AAACGGTTAA GATCAGTGTC GTGCCCAAGA ATCTAGTGCC TGGCATTCGA 
GCCAAGCTGA TCTGTGACTC CAGCTCTAGT AATCCACCGG CGAAGATAAG CTGGTGGAAA 
GATGGCATTC CCGTCGAAGG TCTTAACTTG GCCAATAGGC CCGGTCTCTG GGGCGGTTCG 
GTGTCCACGC TTGAGATGTA CGTTAACATA ACCCAGGATC TGGATGGCAT TGTCTATACC 
TGTCAGAGTC ACAACGAGGT TCTGCAGCGC AGTGTTCACG AAACAATTAG CTTGGATATA 
CTTTATCCCC CCAAATTCGA GACGCCACAG AGCACCACCT TCGTGGGCGT TGAGGGAGCG 
CCGTTCCATG TGGAGCTGCT GGCCAGCGGA AACCCCATGG TAATTACCTA TACCTGGACC 
AAGGACGGCC TGCCCATTAG CAGCAATAGT CTGAGCGGGC AGCGTTTGAT CTCCGACGGA 
CCTCGTCTCA ATATCAGTCG TCTGAGTCGC AATGATGCGG GTGTCTACAT ATGCGAGGCG 
CTCAATAGCC AGGGCACCGC GCTGCTGGAG ATTCAAGTGG CCGTGGAATA TGCCCCCACA 
ATTACGGCGG TTAGCGAGGG TCGCAGTTTT GTGGCCGGCG AACCGGCGGT CTTGGCCTGC 
CACATTCAGG CCCGCCCGCT AGAGGCCGCT CATGTGCGCT GGTCCCGCGA CGGTTATGAT 
CTGGCCACGC GCACCATATC GAGTTTTGAA AATGGAACCG CCCTGCTGCA GATCGCCAGT 
GTGGAGCGCA GTGATATTGG CAACTTTACC TGCATTGTGG ACAACCAAAG AGGAGCTCCC 
GCCGCGCAAA ACGTACTTCT GGTGGTGCAA ACTGCCCCTG AAATTGACCA CTCACCCGGT 
TTCACCCGCT ATGCTGCCCG TTTGGGAGTT CGTGCCCAGC TCATTTGCAG ATCTCTGGCC 
TCGCCGCAGC CCAGTTTCAT CTGGCGTCGT CATGGCAAGG ATCTCAAGAT GCAACGGCGC 
AACAAATTCA AAAGCGTGGA ACGCCAGGTG GATGCCCTGA ACTTTGAGTC GGCTCTGCTA 
ATCGAAAACA CTTCGCCCGA TGATTATGGT CAGTACGAGT GTGTGGTGCG AAATTCGCTG 
GGACAGGCGA GTACCACCCT GGAGTTTAGC AAACCCTCTC GCCCGGATGC TCCGCTGCAG 
CTGAGGGTGG GCAATGTGAG TGATACGGGT GTGGATCTGA ACTGGACTCC CGGCTTCGAT 
GGTGGCATGC AGACATATTT CCGTCTGCGG CTCAAACAGC ACGGCGAGGA TAAGTACAAG 
TACGTGGACG CCAAGCCCGG ACATCAGAAT ATATCCCTTG ATGGCCTGAA GCCAGGAGCA 
ACCTACTACT TCTCCGTGAT GGCCGCCAAT GAAGCGGGAG GCAGTAAATT CATGCCCGAT 
ATCAAGTTGA CCCTCAGCAA GGGATCGCAG CCCCATTCGG CGGAGTACAC CGAAAAAGAC 
GAACTGCCCA ATGTGATGAT CATTGGAATC ACCTCAGCAG CCATGGTTTT GTTGGTTCTG 
AACGCTGCCC TGGTGGCCTG GTTCGTGATC CGCCGGCAGA ACAAATCTCA GAGCGAAGCG 
GAGCCGAGCA ACGATGATGT GTACTCCAAG GATGACAGTC AGTCTGTCTA CAAGCTGCCC 
ATCACTGCTT TGCAGGCGGA TGTTCAGAAG AAAGCAGCCG CCTCCACATA TCTCGTGGAG 
AACGTGGACA TTATCCAGTC GACGGCGTAT CCGCCGAAAT ATCAGGAGAG CTCCATGTGC 
ACCCCGCCCT ATCCGCTCTG CAATCCGGAC TTCACCCGGA CCCTGCCGAA TCCCAAGCGG 
CACAGTCAGC GGAACAGTGC CACGGGCATG ATCGAGGGCA TGCAGATGCG CTCCAAGGAC 
GATCATATGC TGATCTCCAA CGGACTGTAC ATACCATCAC CGTCGCCGGC CTCCTCCCTG 
GTGATCAAGG GTAGCTACAT ATCCTCACCA TCGCCAGCGC CGCCAGCAGA TGGATCATAC 
TTTAATATGA GCGACAAATA TATGTCCTAT CCGCCGGTGA CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788355.1
CDS	396..4103
Translation	MQFWITLTFAVHLATTICHVMAAVTAPTPAAQVAAPPVQKFHLTPHDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSRGGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGANQAKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAHVRWSRDGYDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPDIKLTLSKGSQPHSAEYTEKDELPNVMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAEPSNDDVYSKDDSQSVYKLPITALQADVQKKAAASTYLVENVDIIQSTAYPPKYQESSMCTPPYPLCNPDFTRTLPNPKRHSQRNSATGMIEGMQMRSKDDHMLISNGLYIPSPSPASSLVIKGSYISSPSPAPPADGSYFNMSDKYMSYPPVTY

Target ORF information:

RefSeq Version	NM_176175.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hibris (hbs), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176175.2

ORF Insert Sequence:

ATGCAGTTTT GGATAACTCT GACATTTGCC GTGCACTTGG CTACGACCAT CTGCCACGTA 
ATGGCCGCAG TTACTGCTCC AACTCCGGCT GCTCAGGTGG CAGCCCCACC TGTGCAAAAG 
TTTCACCTGA CGCCCCACGA TCTGCAGATA CTCGAGGGCA CCGACACCCT CCTGCGCTGC 
GAGGTATCGA ACCGGGCGGG CAAAGTCCAG TGGACCAAGG ATGGCTTTGC GCTGGGATTC 
TCGGCCGTAA TTCCCGGATT CCCACGCTAT TCTGTGCTGG TGGATGCCAA ACAGAATGCC 
TACAATCTGC AGATCAAGAA CGCCACGCTG GAGGACGATG CCGAATACCA ATGCCAGGTG 
GGTCCTGCTG CTGGAAATCC CGCCATTCGT GCCAATGCCA AGTTGAGCAT AGTGGCTGCT 
CCGAGTAGCA TCGTTATCGA GGGCTATGCC CGGAATGCCC GAGTGGAGGT GGAGGAGCGC 
CAGAATCTTA CGCTGCACTG TATCGCCGAG AACGCCAATC CGGCGGCGGA GATTGTCTGG 
TTCCAGGGCG AAGTTCCAGT CTCCACGGCC CCCATCGTGA CGGTGAATCA AACTGCCCCG 
AAGCGATTCA CAACTAGCTC CACTTTGCAC CTGCAGCCGC GCGCCGAAGA CGATTACAAG 
GAGTTCTCCT GCGAGGCGCG TCACAAGGCT CTGCCCCCCG ATGTACCGAT GCGCGCCCAG 
GTGCAGCTCT CCGTACTCTA TCCGCCCGGC GCTCCGTTCT TTGAGGGCTA CAGCCAGGGT 
GAGACCCTGC ATCGCGGCCA GGAAGTTCAA ATCGCCTGTC GAAGCCGCGG CGGCAATCCC 
CCGGCCCAAT TGACTTGGTA CCGAAATGGC GTGGCCATCA GTTCCCCACA ACGCACCTCG 
GGCCGTCTGT CGGAGAACGT TTACAAGTTC ACCGCCGCCG CCGAGGACAA TGGGGCTAAT 
TTGGTGTGCG AGGCCAAAAA TCTGCTGGCG ACAACTCCTC TGCGCGCCGA ACTCAATTTG 
ACTGTCCTGT ATGCTCCCAA GGATGTTTAT CTGAGCGGCG CCAATCAGGC CAAGGTCGGC 
GATTCCGTGC AACTAAGCTG CGTGACAGCG CCTTCGAATC CCCAGGCCCG AATTAGCTGG 
TCCATAAATG GCAGACCGCT GGACAATAGC ACCTATAAGA CGACCAGTAG CTCCGACGGT 
GGCTGGGTCA GTAGTTCCAA CATCAGCTTG ACTATAGATT CGCAGAGCAG GACTTTCATC 
GCCGTTTGCC ACGCCCTCAA CACGGAACTC ACTCAGAATG TGGTGGGATC ACACACCGTG 
AATGTGCTTT ATCCCCCTTC GCCACCCTTG TTGACGGGCT ATAATGATGG TGATATCCTT 
ATCTCGGGAT CGATTTTGAA ACTTCAGTGC AGTTCTGCTG GCGGAAATCC TCCACCGACA 
TTGCAGTGGT ACAAAAACGA TAAGATCATT AATGCTCCCT CCAAACTGGT GGACAGTAAA 
ATCACCTCTG AGCTCTCACT TTTGGTAAAC GCCTCCGATA ACAATGCCAT CTATAAGTGC 
AAGGTTCAAA ATGCAGCTAT TGACATACCA CTATTTGCCA CCAAAACTTT GGGAGTTCAT 
TTTCCTCCCG AAACGGTTAA GATCAGTGTC GTGCCCAAGA ATCTAGTGCC TGGCATTCGA 
GCCAAGCTGA TCTGTGACTC CAGCTCTAGT AATCCACCGG CGAAGATAAG CTGGTGGAAA 
GATGGCATTC CCGTCGAAGG TCTTAACTTG GCCAATAGGC CCGGTCTCTG GGGCGGTTCG 
GTGTCCACGC TTGAGATGTA CGTTAACATA ACCCAGGATC TGGATGGCAT TGTCTATACC 
TGTCAGAGTC ACAACGAGGT TCTGCAGCGC AGTGTTCACG AAACAATTAG CTTGGATATA 
CTTTATCCCC CCAAATTCGA GACGCCACAG AGCACCACCT TCGTGGGCGT TGAGGGAGCG 
CCGTTCCATG TGGAGCTGCT GGCCAGCGGA AACCCCATGG TAATTACCTA TACCTGGACC 
AAGGACGGCC TGCCCATTAG CAGCAATAGT CTGAGCGGGC AGCGTTTGAT CTCCGACGGA 
CCTCGTCTCA ATATCAGTCG TCTGAGTCGC AATGATGCGG GTGTCTACAT ATGCGAGGCG 
CTCAATAGCC AGGGCACCGC GCTGCTGGAG ATTCAAGTGG CCGTGGAATA TGCCCCCACA 
ATTACGGCGG TTAGCGAGGG TCGCAGTTTT GTGGCCGGCG AACCGGCGGT CTTGGCCTGC 
CACATTCAGG CCCGCCCGCT AGAGGCCGCT CATGTGCGCT GGTCCCGCGA CGGTTATGAT 
CTGGCCACGC GCACCATATC GAGTTTTGAA AATGGAACCG CCCTGCTGCA GATCGCCAGT 
GTGGAGCGCA GTGATATTGG CAACTTTACC TGCATTGTGG ACAACCAAAG AGGAGCTCCC 
GCCGCGCAAA ACGTACTTCT GGTGGTGCAA ACTGCCCCTG AAATTGACCA CTCACCCGGT 
TTCACCCGCT ATGCTGCCCG TTTGGGAGTT CGTGCCCAGC TCATTTGCAG ATCTCTGGCC 
TCGCCGCAGC CCAGTTTCAT CTGGCGTCGT CATGGCAAGG ATCTCAAGAT GCAACGGCGC 
AACAAATTCA AAAGCGTGGA ACGCCAGGTG GATGCCCTGA ACTTTGAGTC GGCTCTGCTA 
ATCGAAAACA CTTCGCCCGA TGATTATGGT CAGTACGAGT GTGTGGTGCG AAATTCGCTG 
GGACAGGCGA GTACCACCCT GGAGTTTAGC AAACCCTCTC GCCCGGATGC TCCGCTGCAG 
CTGAGGGTGG GCAATGTGAG TGATACGGGT GTGGATCTGA ACTGGACTCC CGGCTTCGAT 
GGTGGCATGC AGACATATTT CCGTCTGCGG CTCAAACAGC ACGGCGAGGA TAAGTACAAG 
TACGTGGACG CCAAGCCCGG ACATCAGAAT ATATCCCTTG ATGGCCTGAA GCCAGGAGCA 
ACCTACTACT TCTCCGTGAT GGCCGCCAAT GAAGCGGGAG GCAGTAAATT CATGCCCGAT 
ATCAAGTTGA CCCTCAGCAA GGGATCGCAG CCCCATTCGG CGGAGTACAC CGAAAAAGAC 
GAACTGCCCA ATGTGATGAT CATTGGAATC ACCTCAGCAG CCATGGTTTT GTTGGTTCTG 
AACGCTGCCC TGGTGGCCTG GTTCGTGATC CGCCGGCAGA ACAAATCTCA GAGCGAAGCG 
GAGCCGAGCA ACGATGATGT GTACTCCAAG GATGACAGTC AGTCTGTCTA CAAGCTGCCC 
ATCACTGCTT TGCAGGCGGA TGTTCAGAAG AAAGCAGCCG CCTCCACATA TCTCGTGGAG 
AACGTGGACA TTATCCAGTC GACGGCGTAT CCGCCGAAAT ATCAGGAGAG CTCCATGTGC 
ACCCCGCCCT ATCCGCTCTG CAATCCGGAC TTCACCCGGA CCCTGCCGAA TCCCAAGCGG 
CACAGTCAGC GGAACAGTGC CACGGGCATG ATCGAGGGCA TGCAGATGCG CTCCAAGGAC 
GATCATATGC TGATCTCCAA CGGACTGTAC ATACCATCAC CGTCGCCGGC CTCCTCCCTG 
GTGATCAAGG GTAGCTACAT ATCCTCACCA TCGCCAGCGC CGCCAGCAGA TGGATCATAC 
TTTAATATGA GCGACAAATA TATGTCCTAT CCGCCGGTGA CTTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hbs ( NM_079963.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524702.3 Drosophila melanogaster hibris (hbs), transcript variant A, mRNA.

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CloneID	OFa11329
Clone ID Related Accession (Same CDS sequence)	NM_079963.4
Accession Version	NM_079963.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hibris, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079963.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGTTTT GGATAACTCT GACATTTGCC GTGCACTTGG CTACGACCAT CTGCCACGTA 
ATGGCCGCAG TTACTGCTCC AACTCCGGCT GCTCAGGTGG CAGCCCCACC TGTGCAAAAG 
TTTCACCTGA CGCCCCACGA TCTGCAGATA CTCGAGGGCA CCGACACCCT CCTGCGCTGC 
GAGGTATCGA ACCGGGCGGG CAAAGTCCAG TGGACCAAGG ATGGCTTTGC GCTGGGATTC 
TCGGCCGTAA TTCCCGGATT CCCACGCTAT TCTGTGCTGG TGGATGCCAA ACAGAATGCC 
TACAATCTGC AGATCAAGAA CGCCACGCTG GAGGACGATG CCGAATACCA ATGCCAGGTG 
GGTCCTGCTG CTGGAAATCC CGCCATTCGT GCCAATGCCA AGTTGAGCAT AGTGGCTGCT 
CCGAGTAGCA TCGTTATCGA GGGCTATGCC CGGAATGCCC GAGTGGAGGT GGAGGAGCGC 
CAGAATCTTA CGCTGCACTG TATCGCCGAG AACGCCAATC CGGCGGCGGA GATTGTCTGG 
TTCCAGGGCG AAGTTCCAGT CTCCACGGCC CCCATCGTGA CGGTGAATCA AACTGCCCCG 
AAGCGATTCA CAACTAGCTC CACTTTGCAC CTGCAGCCGC GCGCCGAAGA CGATTACAAG 
GAGTTCTCCT GCGAGGCGCG TCACAAGGCT CTGCCCCCCG ATGTACCGAT GCGCGCCCAG 
GTGCAGCTCT CCGTACTCTA TCCGCCCGGC GCTCCGTTCT TTGAGGGCTA CAGCCAGGGT 
GAGACCCTGC ATCGCGGCCA GGAAGTTCAA ATCGCCTGTC GAAGCCGCGG CGGCAATCCC 
CCGGCCCAAT TGACTTGGTA CCGAAATGGC GTGGCCATCA GTTCCCCACA ACGCACCTCG 
GGCCGTCTGT CGGAGAACGT TTACAAGTTC ACCGCCGCCG CCGAGGACAA TGGGGCTAAT 
TTGGTGTGCG AGGCCAAAAA TCTGCTGGCG ACAACTCCTC TGCGCGCCGA ACTCAATTTG 
ACTGTCCTGT ATGCTCCCAA GGATGTTTAT CTGAGCGGCG CCAATCAGGC CAAGGTCGGC 
GATTCCGTGC AACTAAGCTG CGTGACAGCG CCTTCGAATC CCCAGGCCCG AATTAGCTGG 
TCCATAAATG GCAGACCGCT GGACAATAGC ACCTATAAGA CGACCAGTAG CTCCGACGGT 
GGCTGGGTCA GTAGTTCCAA CATCAGCTTG ACTATAGATT CGCAGAGCAG GACTTTCATC 
GCCGTTTGCC ACGCCCTCAA CACGGAACTC ACTCAGAATG TGGTGGGATC ACACACCGTG 
AATGTGCTTT ATCCCCCTTC GCCACCCTTG TTGACGGGCT ATAATGATGG TGATATCCTT 
ATCTCGGGAT CGATTTTGAA ACTTCAGTGC AGTTCTGCTG GCGGAAATCC TCCACCGACA 
TTGCAGTGGT ACAAAAACGA TAAGATCATT AATGCTCCCT CCAAACTGGT GGACAGTAAA 
ATCACCTCTG AGCTCTCACT TTTGGTAAAC GCCTCCGATA ACAATGCCAT CTATAAGTGC 
AAGGTTCAAA ATGCAGCTAT TGACATACCA CTATTTGCCA CCAAAACTTT GGGAGTTCAT 
TTTCCTCCCG AAACGGTTAA GATCAGTGTC GTGCCCAAGA ATCTAGTGCC TGGCATTCGA 
GCCAAGCTGA TCTGTGACTC CAGCTCTAGT AATCCACCGG CGAAGATAAG CTGGTGGAAA 
GATGGCATTC CCGTCGAAGG TCTTAACTTG GCCAATAGGC CCGGTCTCTG GGGCGGTTCG 
GTGTCCACGC TTGAGATGTA CGTTAACATA ACCCAGGATC TGGATGGCAT TGTCTATACC 
TGTCAGAGTC ACAACGAGGT TCTGCAGCGC AGTGTTCACG AAACAATTAG CTTGGATATA 
CTTTATCCCC CCAAATTCGA GACGCCACAG AGCACCACCT TCGTGGGCGT TGAGGGAGCG 
CCGTTCCATG TGGAGCTGCT GGCCAGCGGA AACCCCATGG TAATTACCTA TACCTGGACC 
AAGGACGGCC TGCCCATTAG CAGCAATAGT CTGAGCGGGC AGCGTTTGAT CTCCGACGGA 
CCTCGTCTCA ATATCAGTCG TCTGAGTCGC AATGATGCGG GTGTCTACAT ATGCGAGGCG 
CTCAATAGCC AGGGCACCGC GCTGCTGGAG ATTCAAGTGG CCGTGGAATA TGCCCCCACA 
ATTACGGCGG TTAGCGAGGG TCGCAGTTTT GTGGCCGGCG AACCGGCGGT CTTGGCCTGC 
CACATTCAGG CCCGCCCGCT AGAGGCCGCT CATGTGCGCT GGTCCCGCGA CGGTTATGAT 
CTGGCCACGC GCACCATATC GAGTTTTGAA AATGGAACCG CCCTGCTGCA GATCGCCAGT 
GTGGAGCGCA GTGATATTGG CAACTTTACC TGCATTGTGG ACAACCAAAG AGGAGCTCCC 
GCCGCGCAAA ACGTACTTCT GGTGGTGCAA ACTGCCCCTG AAATTGACCA CTCACCCGGT 
TTCACCCGCT ATGCTGCCCG TTTGGGAGTT CGTGCCCAGC TCATTTGCAG ATCTCTGGCC 
TCGCCGCAGC CCAGTTTCAT CTGGCGTCGT CATGGCAAGG ATCTCAAGAT GCAACGGCGC 
AACAAATTCA AAAGCGTGGA ACGCCAGGTG GATGCCCTGA ACTTTGAGTC GGCTCTGCTA 
ATCGAAAACA CTTCGCCCGA TGATTATGGT CAGTACGAGT GTGTGGTGCG AAATTCGCTG 
GGACAGGCGA GTACCACCCT GGAGTTTAGC AAACCCTCTC GCCCGGATGC TCCGCTGCAG 
CTGAGGGTGG GCAATGTGAG TGATACGGGT GTGGATCTGA ACTGGACTCC CGGCTTCGAT 
GGTGGCATGC AGACATATTT CCGTCTGCGG CTCAAACAGC ACGGCGAGGA TAAGTACAAG 
TACGTGGACG CCAAGCCCGG ACATCAGAAT ATATCCCTTG ATGGCCTGAA GCCAGGAGCA 
ACCTACTACT TCTCCGTGAT GGCCGCCAAT GAAGCGGGAG GCAGTAAATT CATGCCCGAT 
ATCAAGTTGA CCCTCAGCAA GGGATCGCAG CCCCATTCGG CGGAGTACAC CGAAAAAGAC 
GAACTGCCCA ATGTGATGAT CATTGGAATC ACCTCAGCAG CCATGGTTTT GTTGGTTCTG 
AACGCTGCCC TGGTGGCCTG GTTCGTGATC CGCCGGCAGA ACAAATCTCA GAGCGAAGCG 
GAGCCGAGCA ACGATGATGT GTACTCCAAG GATGACAGTC AGTCTGTCTA CAAGGCGGAT 
GTTCAGAAGA AAGCAGCCGC CTCCACATAT CTCGTGGAGA ACGTGGACAT TATCCAGTCG 
ACGGCGTATC CGCCGAAATA TCAGGAGAGC TCCATGTGCA CCCCGCCCTA TCCGCTCTGC 
AATCCGGACT TCACCCGGAC CCTGCCGAAT CCCAAGCGGC ACAGTCAGCG GAACAGTGCC 
ACGGGCATGA TCGAGGGCAT GCAGATGCGC TCCAAGGACG ATCATATGCT GATCTCCAAC 
GGACTGTACA TACCATCACC GTCGCCGGCC TCCTCCCTGG TGATCAAGGG TAGCTACATA 
TCCTCACCAT CGCCAGCGCC GCCAGCAGAT GGATCATACT TTAATATGAG CGACAAATAT 
ATGTCCTATC CGCCGGTGAC TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524702.3
CDS	396..4082
Translation	MQFWITLTFAVHLATTICHVMAAVTAPTPAAQVAAPPVQKFHLTPHDLQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARNARVEVEERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPDVPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACRSRGGNPPAQLTWYRNGVAISSPQRTSGRLSENVYKFTAAAEDNGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGANQAKVGDSVQLSCVTAPSNPQARISWSINGRPLDNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVHFPPETVKISVVPKNLVPGIRAKLICDSSSSNPPAKISWWKDGIPVEGLNLANRPGLWGGSVSTLEMYVNITQDLDGIVYTCQSHNEVLQRSVHETISLDILYPPKFETPQSTTFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSNSLSGQRLISDGPRLNISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFVAGEPAVLACHIQARPLEAAHVRWSRDGYDLATRTISSFENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRLKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPDIKLTLSKGSQPHSAEYTEKDELPNVMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAEPSNDDVYSKDDSQSVYKADVQKKAAASTYLVENVDIIQSTAYPPKYQESSMCTPPYPLCNPDFTRTLPNPKRHSQRNSATGMIEGMQMRSKDDHMLISNGLYIPSPSPASSLVIKGSYISSPSPAPPADGSYFNMSDKYMSYPPVTY

Target ORF information:

RefSeq Version	NM_079963.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hibris (hbs), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079963.4

ORF Insert Sequence:

ATGCAGTTTT GGATAACTCT GACATTTGCC GTGCACTTGG CTACGACCAT CTGCCACGTA 
ATGGCCGCAG TTACTGCTCC AACTCCGGCT GCTCAGGTGG CAGCCCCACC TGTGCAAAAG 
TTTCACCTGA CGCCCCACGA TCTGCAGATA CTCGAGGGCA CCGACACCCT CCTGCGCTGC 
GAGGTATCGA ACCGGGCGGG CAAAGTCCAG TGGACCAAGG ATGGCTTTGC GCTGGGATTC 
TCGGCCGTAA TTCCCGGATT CCCACGCTAT TCTGTGCTGG TGGATGCCAA ACAGAATGCC 
TACAATCTGC AGATCAAGAA CGCCACGCTG GAGGACGATG CCGAATACCA ATGCCAGGTG 
GGTCCTGCTG CTGGAAATCC CGCCATTCGT GCCAATGCCA AGTTGAGCAT AGTGGCTGCT 
CCGAGTAGCA TCGTTATCGA GGGCTATGCC CGGAATGCCC GAGTGGAGGT GGAGGAGCGC 
CAGAATCTTA CGCTGCACTG TATCGCCGAG AACGCCAATC CGGCGGCGGA GATTGTCTGG 
TTCCAGGGCG AAGTTCCAGT CTCCACGGCC CCCATCGTGA CGGTGAATCA AACTGCCCCG 
AAGCGATTCA CAACTAGCTC CACTTTGCAC CTGCAGCCGC GCGCCGAAGA CGATTACAAG 
GAGTTCTCCT GCGAGGCGCG TCACAAGGCT CTGCCCCCCG ATGTACCGAT GCGCGCCCAG 
GTGCAGCTCT CCGTACTCTA TCCGCCCGGC GCTCCGTTCT TTGAGGGCTA CAGCCAGGGT 
GAGACCCTGC ATCGCGGCCA GGAAGTTCAA ATCGCCTGTC GAAGCCGCGG CGGCAATCCC 
CCGGCCCAAT TGACTTGGTA CCGAAATGGC GTGGCCATCA GTTCCCCACA ACGCACCTCG 
GGCCGTCTGT CGGAGAACGT TTACAAGTTC ACCGCCGCCG CCGAGGACAA TGGGGCTAAT 
TTGGTGTGCG AGGCCAAAAA TCTGCTGGCG ACAACTCCTC TGCGCGCCGA ACTCAATTTG 
ACTGTCCTGT ATGCTCCCAA GGATGTTTAT CTGAGCGGCG CCAATCAGGC CAAGGTCGGC 
GATTCCGTGC AACTAAGCTG CGTGACAGCG CCTTCGAATC CCCAGGCCCG AATTAGCTGG 
TCCATAAATG GCAGACCGCT GGACAATAGC ACCTATAAGA CGACCAGTAG CTCCGACGGT 
GGCTGGGTCA GTAGTTCCAA CATCAGCTTG ACTATAGATT CGCAGAGCAG GACTTTCATC 
GCCGTTTGCC ACGCCCTCAA CACGGAACTC ACTCAGAATG TGGTGGGATC ACACACCGTG 
AATGTGCTTT ATCCCCCTTC GCCACCCTTG TTGACGGGCT ATAATGATGG TGATATCCTT 
ATCTCGGGAT CGATTTTGAA ACTTCAGTGC AGTTCTGCTG GCGGAAATCC TCCACCGACA 
TTGCAGTGGT ACAAAAACGA TAAGATCATT AATGCTCCCT CCAAACTGGT GGACAGTAAA 
ATCACCTCTG AGCTCTCACT TTTGGTAAAC GCCTCCGATA ACAATGCCAT CTATAAGTGC 
AAGGTTCAAA ATGCAGCTAT TGACATACCA CTATTTGCCA CCAAAACTTT GGGAGTTCAT 
TTTCCTCCCG AAACGGTTAA GATCAGTGTC GTGCCCAAGA ATCTAGTGCC TGGCATTCGA 
GCCAAGCTGA TCTGTGACTC CAGCTCTAGT AATCCACCGG CGAAGATAAG CTGGTGGAAA 
GATGGCATTC CCGTCGAAGG TCTTAACTTG GCCAATAGGC CCGGTCTCTG GGGCGGTTCG 
GTGTCCACGC TTGAGATGTA CGTTAACATA ACCCAGGATC TGGATGGCAT TGTCTATACC 
TGTCAGAGTC ACAACGAGGT TCTGCAGCGC AGTGTTCACG AAACAATTAG CTTGGATATA 
CTTTATCCCC CCAAATTCGA GACGCCACAG AGCACCACCT TCGTGGGCGT TGAGGGAGCG 
CCGTTCCATG TGGAGCTGCT GGCCAGCGGA AACCCCATGG TAATTACCTA TACCTGGACC 
AAGGACGGCC TGCCCATTAG CAGCAATAGT CTGAGCGGGC AGCGTTTGAT CTCCGACGGA 
CCTCGTCTCA ATATCAGTCG TCTGAGTCGC AATGATGCGG GTGTCTACAT ATGCGAGGCG 
CTCAATAGCC AGGGCACCGC GCTGCTGGAG ATTCAAGTGG CCGTGGAATA TGCCCCCACA 
ATTACGGCGG TTAGCGAGGG TCGCAGTTTT GTGGCCGGCG AACCGGCGGT CTTGGCCTGC 
CACATTCAGG CCCGCCCGCT AGAGGCCGCT CATGTGCGCT GGTCCCGCGA CGGTTATGAT 
CTGGCCACGC GCACCATATC GAGTTTTGAA AATGGAACCG CCCTGCTGCA GATCGCCAGT 
GTGGAGCGCA GTGATATTGG CAACTTTACC TGCATTGTGG ACAACCAAAG AGGAGCTCCC 
GCCGCGCAAA ACGTACTTCT GGTGGTGCAA ACTGCCCCTG AAATTGACCA CTCACCCGGT 
TTCACCCGCT ATGCTGCCCG TTTGGGAGTT CGTGCCCAGC TCATTTGCAG ATCTCTGGCC 
TCGCCGCAGC CCAGTTTCAT CTGGCGTCGT CATGGCAAGG ATCTCAAGAT GCAACGGCGC 
AACAAATTCA AAAGCGTGGA ACGCCAGGTG GATGCCCTGA ACTTTGAGTC GGCTCTGCTA 
ATCGAAAACA CTTCGCCCGA TGATTATGGT CAGTACGAGT GTGTGGTGCG AAATTCGCTG 
GGACAGGCGA GTACCACCCT GGAGTTTAGC AAACCCTCTC GCCCGGATGC TCCGCTGCAG 
CTGAGGGTGG GCAATGTGAG TGATACGGGT GTGGATCTGA ACTGGACTCC CGGCTTCGAT 
GGTGGCATGC AGACATATTT CCGTCTGCGG CTCAAACAGC ACGGCGAGGA TAAGTACAAG 
TACGTGGACG CCAAGCCCGG ACATCAGAAT ATATCCCTTG ATGGCCTGAA GCCAGGAGCA 
ACCTACTACT TCTCCGTGAT GGCCGCCAAT GAAGCGGGAG GCAGTAAATT CATGCCCGAT 
ATCAAGTTGA CCCTCAGCAA GGGATCGCAG CCCCATTCGG CGGAGTACAC CGAAAAAGAC 
GAACTGCCCA ATGTGATGAT CATTGGAATC ACCTCAGCAG CCATGGTTTT GTTGGTTCTG 
AACGCTGCCC TGGTGGCCTG GTTCGTGATC CGCCGGCAGA ACAAATCTCA GAGCGAAGCG 
GAGCCGAGCA ACGATGATGT GTACTCCAAG GATGACAGTC AGTCTGTCTA CAAGGCGGAT 
GTTCAGAAGA AAGCAGCCGC CTCCACATAT CTCGTGGAGA ACGTGGACAT TATCCAGTCG 
ACGGCGTATC CGCCGAAATA TCAGGAGAGC TCCATGTGCA CCCCGCCCTA TCCGCTCTGC 
AATCCGGACT TCACCCGGAC CCTGCCGAAT CCCAAGCGGC ACAGTCAGCG GAACAGTGCC 
ACGGGCATGA TCGAGGGCAT GCAGATGCGC TCCAAGGACG ATCATATGCT GATCTCCAAC 
GGACTGTACA TACCATCACC GTCGCCGGCC TCCTCCCTGG TGATCAAGGG TAGCTACATA 
TCCTCACCAT CGCCAGCGCC GCCAGCAGAT GGATCATACT TTAATATGAG CGACAAATAT 
ATGTCCTATC CGCCGGTGAC TTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hbs cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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