Optix cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Optix
Entrez Gene ID	44108
Full Name	CG18455 gene product from transcript CG18455-RC
Synonyms	CG18455,D-Six3,Dmel\CG18455,Dsix3,OPTIX,Six3,anon-WO0153538.79,opt,optix,opx,six3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 44A3-44A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_165584.2	NP_724640.1	optix, isoform B
NM_001273864.2	NP_001260793.1	optix, isoform C
NM_079956.3	NP_524695.2	optix, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	six3b	NP_571438.1
	LOC717027	XP_001108191.1
Drosophila melanogaster (fruit fly)	Optix	NP_001260793.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Anteroposterior patterning of Drosophila ocelli requires an anti-repressor mechanism within the hh pathway mediated by the Six3 gene Optix.
Title: Anteroposterior patterning of Drosophila ocelli requires an anti-repressor mechanism within the hh pathway mediated by the Six3 gene Optix.

optix functions together with hh to regulate dpp in the morphogenetic furrow, serving as a link between the RD network and the patterning pathways controlling normal retinal development
Title: optix functions as a link between the retinal determination network and the dpp pathway to control morphogenetic furrow progression in Drosophila.

Data suggest that during early phases of eye development, So and Optix function, in part, to repress the transcription of non-retinal selector genes, thereby allowing induction of the retina to proceed.
Title: Dual transcriptional activities of SIX proteins define their roles in normal and ectopic eye development.

different cofactors likely mediate unique functions of Sine oculis and Optix during the development of the fly eye
Title: Fly SIX-type homeodomain proteins Sine oculis and Optix partner with different cofactors during eye development.

Sine oculis mutation within the Six domain does not abolish DNA-protein interactions, but alters co-factor binding specificity to resemble that of Optix
Title: Partner specificity is essential for proper function of the SIX-type homeodomain proteins Sine oculis and Optix during fly eye development.

The following Optix gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Optix cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11325	NM_079956.3 Latest version!	Drosophila melanogaster optix, transcript variant A (Optix), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00
OFa11326	NM_165584.2 Latest version!	Drosophila melanogaster optix, transcript variant B (Optix), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$174.30 $249.00
OFa12213	NM_001273864.2 Latest version!	Drosophila melanogaster optix, transcript variant C (Optix), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Optix ( NM_079956.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524695.2 Drosophila melanogaster optix, transcript variant A (Optix), mRNA.

Price & Availability↑

CloneID	OFa11325
Clone ID Related Accession (Same CDS sequence)	NM_079956.3
Accession Version	NM_079956.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optix, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_079956.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTTG GACCGACGGA GGGCAAACAG CCGCCCTCAG AGAGCTTCTC GCCCACGCAC 
CACCAGATTA TAGCACCCAG CCCCATCCTG GCCGTTCCAA CGCTGGCCTT CTCCGCCGCC 
CAGGTGGAGA TCGTTTGCAA GACGCTCGAG GACTCCGGCG ACATCGAGCG GTTGGCCCGC 
TTCCTCTGGA GCCTGCCGGT GGCCCTGCCC AACATGCACG AGATCCTCAA CTGCGAGGCG 
GTGCTCCGTG CCCGCGCAGT GGTCGCCTAC CATGTGGGCA ACTTCAGGGA ACTTTATGCA 
ATAATAGAGA ATCATAAGTT TACTAAGGCG TCCTACGGCA AACTGCAGGC CATGTGGCTG 
GAGGCCCACT ACATTGAGGC CGAGAAGCTG CGCGGTCGAT CACTGGGCCC CGTGGACAAG 
TATCGGGTGC GAAAGAAGTT CCCCCTGCCG CCGACGATCT GGGATGGCGA GCAGAAGACG 
CACTGCTTCA AGGAGCGCAC GCGGAGCCTA CTGCGCGAAT GGTACCTACA GGATCCCTAC 
CCGAATCCCA CCAAGAAGCG GGAGCTGGCC AAGGCCACCG GCCTGAATCC CACGCAAGTG 
GGCAACTGGT TCAAGAACCG CCGCCAGCGG GATCGGGCTG CTGCGGCCAA GAATCGCATC 
CAGCATAGCC AGAACAGCTC GGGAATGGGC TGTCGGAGCC GGCGGGCGGA CGGAGCTGCC 
TCACCAACGC CGTCGGACAG CTCCGACTCG GACATCTCAC TGGGCACCCA CTCGCCGGTG 
CCGAGCAGCC TGCAGCTGCA GCATAGTCCG GGCAGCACCT CCAACGGCGC CAACGACCGC 
GAGGAGAGCT TGAGCGTGGA CGACGACAAG CCGCGGGATC TGAGCGGATC GCTGCCACTG 
CCCCTCTCGC TGCCCCTGCC GCTGGCCTCG CCCACCCACA CGCCGCCCCA ACTGCCGCCG 
GGCTACGGGG GCGGGGCGGG CGCAGGACCC GGAGGACCTC TGACCGGTCC GGGCTGTCTG 
CCACCCTTCA AGCTGGACGC GGCCACCAGT CTGTTCAGCG CCGGCTGTTA CCTGCAGAGC 
TTCAGCAACC TGAAGGAGAT GTCGCAGCAG TTTCCCATCC AGCCGATTGT CCTGCGTCCG 
CATCCGCAGC TGCCGCAGTC GCTGGCACTG AACGGCGCAT CCGGCGGACC GCCACTGCAT 
CACCCGGCCT ACGCGGCTGC CTACAGTGTG GAGTGCGTTC CAGGTGGTCA TGGACCACCG 
CATCCGCCGC CGAAGCTGAG GATCAACTCC CCGGAGAAGC TCAACTCCAC GGCAGTGGCG 
GCGGCAGCAT CCGTCGGAGG AGGAGGTGGC AACCAACACC ATGAGCCCAC CACGACCGGC 
TACCATCACA GTGGCCAACT GATGCTGCAT CGGCCCTTCT CCACGTCGCC GGAGCTGAAG 
CACAGTGCTC CCGAGATCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524695.2
CDS	366..1829
Translation	MAVGPTEGKQPPSESFSPTHHQIIAPSPILAVPTLAFSAAQVEIVCKTLEDSGDIERLARFLWSLPVALPNMHEILNCEAVLRARAVVAYHVGNFRELYAIIENHKFTKASYGKLQAMWLEAHYIEAEKLRGRSLGPVDKYRVRKKFPLPPTIWDGEQKTHCFKERTRSLLREWYLQDPYPNPTKKRELAKATGLNPTQVGNWFKNRRQRDRAAAAKNRIQHSQNSSGMGCRSRRADGAASPTPSDSSDSDISLGTHSPVPSSLQLQHSPGSTSNGANDREESLSVDDDKPRDLSGSLPLPLSLPLPLASPTHTPPQLPPGYGGGAGAGPGGPLTGPGCLPPFKLDAATSLFSAGCYLQSFSNLKEMSQQFPIQPIVLRPHPQLPQSLALNGASGGPPLHHPAYAAAYSVECVPGGHGPPHPPPKLRINSPEKLNSTAVAAAASVGGGGGNQHHEPTTTGYHHSGQLMLHRPFSTSPELKHSAPEIT

Target ORF information:

RefSeq Version	NM_079956.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optix, transcript variant A (Optix), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079956.3

ORF Insert Sequence:

ATGGCCGTTG GACCGACGGA GGGCAAACAG CCGCCCTCAG AGAGCTTCTC GCCCACGCAC 
CACCAGATTA TAGCACCCAG CCCCATCCTG GCCGTTCCAA CGCTGGCCTT CTCCGCCGCC 
CAGGTGGAGA TCGTTTGCAA GACGCTCGAG GACTCCGGCG ACATCGAGCG GTTGGCCCGC 
TTCCTCTGGA GCCTGCCGGT GGCCCTGCCC AACATGCACG AGATCCTCAA CTGCGAGGCG 
GTGCTCCGTG CCCGCGCAGT GGTCGCCTAC CATGTGGGCA ACTTCAGGGA ACTTTATGCA 
ATAATAGAGA ATCATAAGTT TACTAAGGCG TCCTACGGCA AACTGCAGGC CATGTGGCTG 
GAGGCCCACT ACATTGAGGC CGAGAAGCTG CGCGGTCGAT CACTGGGCCC CGTGGACAAG 
TATCGGGTGC GAAAGAAGTT CCCCCTGCCG CCGACGATCT GGGATGGCGA GCAGAAGACG 
CACTGCTTCA AGGAGCGCAC GCGGAGCCTA CTGCGCGAAT GGTACCTACA GGATCCCTAC 
CCGAATCCCA CCAAGAAGCG GGAGCTGGCC AAGGCCACCG GCCTGAATCC CACGCAAGTG 
GGCAACTGGT TCAAGAACCG CCGCCAGCGG GATCGGGCTG CTGCGGCCAA GAATCGCATC 
CAGCATAGCC AGAACAGCTC GGGAATGGGC TGTCGGAGCC GGCGGGCGGA CGGAGCTGCC 
TCACCAACGC CGTCGGACAG CTCCGACTCG GACATCTCAC TGGGCACCCA CTCGCCGGTG 
CCGAGCAGCC TGCAGCTGCA GCATAGTCCG GGCAGCACCT CCAACGGCGC CAACGACCGC 
GAGGAGAGCT TGAGCGTGGA CGACGACAAG CCGCGGGATC TGAGCGGATC GCTGCCACTG 
CCCCTCTCGC TGCCCCTGCC GCTGGCCTCG CCCACCCACA CGCCGCCCCA ACTGCCGCCG 
GGCTACGGGG GCGGGGCGGG CGCAGGACCC GGAGGACCTC TGACCGGTCC GGGCTGTCTG 
CCACCCTTCA AGCTGGACGC GGCCACCAGT CTGTTCAGCG CCGGCTGTTA CCTGCAGAGC 
TTCAGCAACC TGAAGGAGAT GTCGCAGCAG TTTCCCATCC AGCCGATTGT CCTGCGTCCG 
CATCCGCAGC TGCCGCAGTC GCTGGCACTG AACGGCGCAT CCGGCGGACC GCCACTGCAT 
CACCCGGCCT ACGCGGCTGC CTACAGTGTG GAGTGCGTTC CAGGTGGTCA TGGACCACCG 
CATCCGCCGC CGAAGCTGAG GATCAACTCC CCGGAGAAGC TCAACTCCAC GGCAGTGGCG 
GCGGCAGCAT CCGTCGGAGG AGGAGGTGGC AACCAACACC ATGAGCCCAC CACGACCGGC 
TACCATCACA GTGGCCAACT GATGCTGCAT CGGCCCTTCT CCACGTCGCC GGAGCTGAAG 
CACAGTGCTC CCGAGATCAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Optix ( NM_165584.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_724640.1 Drosophila melanogaster optix, transcript variant B (Optix), mRNA.

Price & Availability↑

CloneID	OFa11326
Clone ID Related Accession (Same CDS sequence)	NM_165584.2
Accession Version	NM_165584.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optix, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_165584.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCTGTC GGAGCCGGCG GGCGGACGGA GCTGCCTCAC CAACGCCGTC GGACAGCTCC 
GACTCGGACA TCTCACTGGG CACCCACTCG CCGGTGCCGA GCAGCCTGCA GCTGCAGCAT 
AGTCCGGGCA GCACCTCCAA CGGCGCCAAC GACCGCGAGG AGAGCTTGAG CGTGGACGAC 
GACAAGCCGC GGGATCTGAG CGGATCGCTG CCACTGCCCC TCTCGCTGCC CCTGCCGCTG 
GCCTCGCCCA CCCACACGCC GCCCCAACTG CCGCCGGGCT ACGGGGGCGG GGCGGGCGCA 
GGACCCGGAG GACCTCTGAC CGGTCCGGGC TGTCTGCCAC CCTTCAAGCT GGACGCGGCC 
ACCAGTCTGT TCAGCGCCGG CTGTTACCTG CAGAGCTTCA GCAACCTGAA GGAGATGTCG 
CAGCAGTTTC CCATCCAGCC GATTGTCCTG CGTCCGCATC CGCAGCTGCC GCAGTCGCTG 
GCACTGAACG GCGCATCCGG CGGACCGCCA CTGCATCACC CGGCCTACGC GGCTGCCTAC 
AGTGTGGAGT GCGTTCCAGG TGGTCATGGA CCACCGCATC CGCCGCCGAA GCTGAGGATC 
AACTCCCCGG AGAAGCTCAA CTCCACGGCA GTGGCGGCGG CAGCATCCGT CGGAGGAGGA 
GGTGGCAACC AACACCATGA GCCCACCACG ACCGGCTACC ATCACAGTGG CCAACTGATG 
CTGCATCGGC CCTTCTCCAC GTCGCCGGAG CTGAAGCACA GTGCTCCCGA GATCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_724640.1
CDS	162..941
Translation	MGCRSRRADGAASPTPSDSSDSDISLGTHSPVPSSLQLQHSPGSTSNGANDREESLSVDDDKPRDLSGSLPLPLSLPLPLASPTHTPPQLPPGYGGGAGAGPGGPLTGPGCLPPFKLDAATSLFSAGCYLQSFSNLKEMSQQFPIQPIVLRPHPQLPQSLALNGASGGPPLHHPAYAAAYSVECVPGGHGPPHPPPKLRINSPEKLNSTAVAAAASVGGGGGNQHHEPTTTGYHHSGQLMLHRPFSTSPELKHSAPEIT

Target ORF information:

RefSeq Version	NM_165584.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optix, transcript variant B (Optix), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165584.2

ORF Insert Sequence:

ATGGGCTGTC GGAGCCGGCG GGCGGACGGA GCTGCCTCAC CAACGCCGTC GGACAGCTCC 
GACTCGGACA TCTCACTGGG CACCCACTCG CCGGTGCCGA GCAGCCTGCA GCTGCAGCAT 
AGTCCGGGCA GCACCTCCAA CGGCGCCAAC GACCGCGAGG AGAGCTTGAG CGTGGACGAC 
GACAAGCCGC GGGATCTGAG CGGATCGCTG CCACTGCCCC TCTCGCTGCC CCTGCCGCTG 
GCCTCGCCCA CCCACACGCC GCCCCAACTG CCGCCGGGCT ACGGGGGCGG GGCGGGCGCA 
GGACCCGGAG GACCTCTGAC CGGTCCGGGC TGTCTGCCAC CCTTCAAGCT GGACGCGGCC 
ACCAGTCTGT TCAGCGCCGG CTGTTACCTG CAGAGCTTCA GCAACCTGAA GGAGATGTCG 
CAGCAGTTTC CCATCCAGCC GATTGTCCTG CGTCCGCATC CGCAGCTGCC GCAGTCGCTG 
GCACTGAACG GCGCATCCGG CGGACCGCCA CTGCATCACC CGGCCTACGC GGCTGCCTAC 
AGTGTGGAGT GCGTTCCAGG TGGTCATGGA CCACCGCATC CGCCGCCGAA GCTGAGGATC 
AACTCCCCGG AGAAGCTCAA CTCCACGGCA GTGGCGGCGG CAGCATCCGT CGGAGGAGGA 
GGTGGCAACC AACACCATGA GCCCACCACG ACCGGCTACC ATCACAGTGG CCAACTGATG 
CTGCATCGGC CCTTCTCCAC GTCGCCGGAG CTGAAGCACA GTGCTCCCGA GATCACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Optix ( NM_001273864.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260793.1 Drosophila melanogaster optix, transcript variant C (Optix), mRNA.

Price & Availability↑

CloneID	OFa12213
Clone ID Related Accession (Same CDS sequence)	NM_001273864.2
Accession Version	NM_001273864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	optix, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001273864.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGTTG GACCGACGGA GGGCAAACAG CCGCCCTCAG AGAGCTTCTC GCCCACGCAC 
CACCAGATTA TAGCACCCAG CCCCATCCTG GCCGTTCCAA CGCTGGCCTT CTCCGCCGCC 
CAGGTGGAGA TCGTTTGCAA GACGCTCGAG GACTCCGGCG ACATCGAGCG GTTGGCCCGC 
TTCCTCTGGA GCCTGCCGGT GGCCCTGCCC AACATGCACG AGATCCTCAA CTGCGAGGCG 
GTGCTCCGTG CCCGCGCAGT GGTCGCCTAC CATGTGGGCA ACTTCAGGGA ACTTTATGCA 
ATAATAGAGA ATCATAAGTT TACTAAGGCG TCCTACGGCA AACTGCAGGC CATGTGGCTG 
GAGGCCCACT ACATTGAGGC CGAGAAGCTG CGCGGTCGAT CACTGGGCCC CGTGGACAAG 
TATCGGGTGC GAAAGAAGTT CCCCCTGCCG CCGACGATCT GGGATGGCGA GCAGAAGACG 
CACTGCTTCA AGGAGCGCAC GCGGAGCCTA CTGCGCGAAT GGTACCTACA GGATCCCTAC 
CCGAATCCCA CCAAGAAGCG GGAGCTGGCC AAGGCCACCG GCCTGAATCC CACGCAAGTG 
GGCAACTGGT TCAAGAACCG CCGCCAGCGG GATCGGGCTG CTGCGGCCAA GAATCGCATC 
CAGCATAGCC AGAACAGCTC GGGAATGGGC TGTCGGAGCC GGCGGGCGGA CGGAGCTGCC 
TCACCAACGC CGTCGGACAG CTCCGACTCG GACATCTCAC TGGGCACCCA CTCGCCGGTG 
CCGAGCAGCC TGCAGCTGCA GCATAGTCCG GGCAGCACCT CCAACGGCGC CAACGACCGC 
GAGGAGAGCT TGAGCGTGGA CGACGACAAG CCGCGGGATC TGAGCGGATC GCTGCCACTG 
CCCCTCTCGC TGCCCCTGCC GCTGGCCTCG CCCACCCACA CGCCGCCCCA ACTGCCGCCG 
GGCTACGGGG GCGGGGCGGG CGCAGGACCC GGAGGACCTC TGACCGGTCC GGGCTGTCTG 
CCACCCTTCA AGCTGGACGC GGCCACCAGT CTGTTCAGCG CCGGCTGTTA CCTGCAGAGC 
TTCAGCAACC TGAAGGAGAT GTCGCAGCAG TTTCCCATCC AGCCGATTGT CCTGCGTCCG 
CATCCGCAGC TGCCGCAGTC GCTGGCACTG AACGGCGCAT CCGGCGGACC GCCACTGCAT 
CACCCGGCCT ACGCGGCTGC CTACAGTGTG GAGTGCGTTC CAGGTGGTCA TGGACCACCG 
CATCCGCCGC CGAAGCTGAG GATCAACTCC CCGGAGAAGC TCAACTCCAC GGCAGTGGCG 
GCGGCAGCAT CCGTCGGAGG AGGAGGTGGC AACCAACACC ATGAGCCCAC CACGACCGGC 
TACCATCACA GTGGCCAACT GATGCTGCAT CGGCCCTTCT CCACGTCGCC GGAGCTGAAG 
CACAGTGCTC CCGAGATCAC ATGATATCAG CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260793.1
CDS	676..2154
Translation	MAVGPTEGKQPPSESFSPTHHQIIAPSPILAVPTLAFSAAQVEIVCKTLEDSGDIERLARFLWSLPVALPNMHEILNCEAVLRARAVVAYHVGNFRELYAIIENHKFTKASYGKLQAMWLEAHYIEAEKLRGRSLGPVDKYRVRKKFPLPPTIWDGEQKTHCFKERTRSLLREWYLQDPYPNPTKKRELAKATGLNPTQVGNWFKNRRQRDRAAAAKNRIQHSQNSSGMGCRSRRADGAASPTPSDSSDSDISLGTHSPVPSSLQLQHSPGSTSNGANDREESLSVDDDKPRDLSGSLPLPLSLPLPLASPTHTPPQLPPGYGGGAGAGPGGPLTGPGCLPPFKLDAATSLFSAGCYLQSFSNLKEMSQQFPIQPIVLRPHPQLPQSLALNGASGGPPLHHPAYAAAYSVECVPGGHGPPHPPPKLRINSPEKLNSTAVAAAASVGGGGGNQHHEPTTTGYHHSGQLMLHRPFSTSPELKHSAPEIT*YQRV

Target ORF information:

RefSeq Version	NM_001273864.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster optix, transcript variant C (Optix), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273864.2

ORF Insert Sequence:

ATGGCCGTTG GACCGACGGA GGGCAAACAG CCGCCCTCAG AGAGCTTCTC GCCCACGCAC 
CACCAGATTA TAGCACCCAG CCCCATCCTG GCCGTTCCAA CGCTGGCCTT CTCCGCCGCC 
CAGGTGGAGA TCGTTTGCAA GACGCTCGAG GACTCCGGCG ACATCGAGCG GTTGGCCCGC 
TTCCTCTGGA GCCTGCCGGT GGCCCTGCCC AACATGCACG AGATCCTCAA CTGCGAGGCG 
GTGCTCCGTG CCCGCGCAGT GGTCGCCTAC CATGTGGGCA ACTTCAGGGA ACTTTATGCA 
ATAATAGAGA ATCATAAGTT TACTAAGGCG TCCTACGGCA AACTGCAGGC CATGTGGCTG 
GAGGCCCACT ACATTGAGGC CGAGAAGCTG CGCGGTCGAT CACTGGGCCC CGTGGACAAG 
TATCGGGTGC GAAAGAAGTT CCCCCTGCCG CCGACGATCT GGGATGGCGA GCAGAAGACG 
CACTGCTTCA AGGAGCGCAC GCGGAGCCTA CTGCGCGAAT GGTACCTACA GGATCCCTAC 
CCGAATCCCA CCAAGAAGCG GGAGCTGGCC AAGGCCACCG GCCTGAATCC CACGCAAGTG 
GGCAACTGGT TCAAGAACCG CCGCCAGCGG GATCGGGCTG CTGCGGCCAA GAATCGCATC 
CAGCATAGCC AGAACAGCTC GGGAATGGGC TGTCGGAGCC GGCGGGCGGA CGGAGCTGCC 
TCACCAACGC CGTCGGACAG CTCCGACTCG GACATCTCAC TGGGCACCCA CTCGCCGGTG 
CCGAGCAGCC TGCAGCTGCA GCATAGTCCG GGCAGCACCT CCAACGGCGC CAACGACCGC 
GAGGAGAGCT TGAGCGTGGA CGACGACAAG CCGCGGGATC TGAGCGGATC GCTGCCACTG 
CCCCTCTCGC TGCCCCTGCC GCTGGCCTCG CCCACCCACA CGCCGCCCCA ACTGCCGCCG 
GGCTACGGGG GCGGGGCGGG CGCAGGACCC GGAGGACCTC TGACCGGTCC GGGCTGTCTG 
CCACCCTTCA AGCTGGACGC GGCCACCAGT CTGTTCAGCG CCGGCTGTTA CCTGCAGAGC 
TTCAGCAACC TGAAGGAGAT GTCGCAGCAG TTTCCCATCC AGCCGATTGT CCTGCGTCCG 
CATCCGCAGC TGCCGCAGTC GCTGGCACTG AACGGCGCAT CCGGCGGACC GCCACTGCAT 
CACCCGGCCT ACGCGGCTGC CTACAGTGTG GAGTGCGTTC CAGGTGGTCA TGGACCACCG 
CATCCGCCGC CGAAGCTGAG GATCAACTCC CCGGAGAAGC TCAACTCCAC GGCAGTGGCG 
GCGGCAGCAT CCGTCGGAGG AGGAGGTGGC AACCAACACC ATGAGCCCAC CACGACCGGC 
TACCATCACA GTGGCCAACT GATGCTGCAT CGGCCCTTCT CCACGTCGCC GGAGCTGAAG 
CACAGTGCTC CCGAGATCAC ATGATATCAG CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

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Optix cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

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