sbb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sbb
Entrez Gene ID	44027
Full Name	scribbler
Synonyms	Bks,Brakeless,CG5580,Dmel\CG5580,GM04742,Group IIa,Sbb,bks,disc6,l(2)04440,mtv,sbb/mtv
General protein information	Preferred Names scribbler Names CG5580 gene product from transcript CG5580-RG CG5580-PC CG5580-PD CG5580-PE CG5580-PF CG5580-PG CG5580-PH CG5580-PJ brakeless master of thickveins mother of thick-vein sbb-PC sbb-PD sbb-PE sbb-PF sbb-PG sbb-PH sbb-PJ
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55C2-55C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169718.3	NP_001163189.1	scribbler, isoform D
NM_001169719.2	NP_001163190.1	scribbler, isoform E
NM_001274145.1	NP_001261074.1	scribbler, isoform J
NM_206162.4	NP_995884.1	scribbler, isoform C
NM_206161.3	NP_995883.1	scribbler, isoform F
NM_001169720.2	NP_001163191.1	scribbler, isoform G
NM_079939.5	NP_524678.3	scribbler, isoform H

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data provide support for a Brakeless activator function that regulates transcription by interacting with Med19
Title: The Brakeless co-regulator can directly activate and repress transcription in early Drosophila embryos.

Sbb is associated with wing size.
Title: Alteration of wing size through over-expression of scribbler isoforms.

Scribble is part of the Stbm/Vang PCP complex and further suggest that it might act as an effector of Stbm/Vang during PCP establishment.
Title: The apical/basal-polarity determinant Scribble cooperates with the PCP core factor Stbm/Vang and functions as one of its effectors.

Brakeless is recruited to the Kr and kni cis-regulatory DNA modules, and represses transcription when tethered to DNA.
Title: Drosophila brakeless interacts with atrophin and is required for tailless-mediated transcriptional repression in early embryos.

mutations in Atro and Sbb have similar phenotypes, including upregulation of the same genes in imaginal discs, which suggests that Sbb cooperates with Atro to provide repressive activity
Title: Genetic interactions among scribbler, Atrophin and groucho in Drosophila uncover links in transcriptional repression.

The following sbb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sbb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11314	NM_206161.3 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa11315	NM_206162.4 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa11315	NM_001169719.2 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa11313	NM_001169718.3 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa11313	NM_079939.5 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa12207	NM_001169720.2 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa12208	NM_001274145.1 Latest version!	Drosophila melanogaster scribbler (sbb), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sbb ( NM_206161.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995883.1 Drosophila melanogaster scribbler (sbb), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa11314
Clone ID Related Accession (Same CDS sequence)	NM_206161.3
Accession Version	NM_206161.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_206161.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAA TGAGACGTAA CGAGGCGAAC ACTAGCCACC ACCCGAATAA GGTGGCCCTG 
AATAGCAGCA ACGTAGCAAC GGAAAGCAAT ATTAAGAATA ATAAATTGTT AGCTAATTTA 
AGTGCGGCAG GAGCAGCTAC AGCTACAACG GCAACAACAG GAACAGCAAC AACAACGACG 
GCGACAACAG CGAATCAAGC GCTGAATTTC AATAACAAAA CGAAAGCAAC AGCAAACGCG 
ACTGCAGCAT CGGCGAATAA TCGGCACAAT AACAACAACA ATTCCAGTGC GATCAAGAAG 
CACACAAATA CGAAACAACT AGCTGGCAAG AGCCCCGCGT GCAACTCCTC CTCCTCGTCG 
CTCTCCTCGT CGAGCAGCTC CAACTCGAGC GAGTCGAAGG ACACGAACTT TGAGTACGAG 
GACGAGTGGA ATATTGGCGG CATACCAGAG CTGCTGGACG ACTTGGACGC GGATATTGAG 
AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC CAGGCTACCG CGCTAAATGC CAAGCAGGCA 
AACAGCTCCT CCACATCCTC CTCATCATCC TCCAAGGGCG GCGCTTCATC CTCGTCATCC 
TCAGCAGCAG CGACGGGATC ATCATCGTCG CATAAATCGC ACAAAACCAC ATTGCACAGC 
AATTTGTCGG CCACATCGCC AACCACAATT AAGTTCACAC GCCAACCGGT GGCCATTGGC 
GGAGCTAACT CCTCCTCATC TTCATCAGCA GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC 
GTGGTGGCCA AGGGATCATC TTCTTCCTCG TCCTCCACAT CTTCCTCATC TTCGGGGAAG 
CATCATCACC ATCACCACCA TCACTCAAAC TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC 
TACAAGTCCG CTTTGGTGGC TCAACTGAAC AGTCCGAGTC CACTGAACAG TAACTCCAAG 
TCCCTGAGTG GATCGGGGAG TGGAAGCGGG AACACAAACG GAGCAGCGGG AGCTGGAGCC 
GGCAGCACAT TGTCATCCTC GACATTTGCC GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT 
TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG GCTGGCAGTG GACAACAGGG CTCCAAATTC 
TCCGCTGGCG GCATGTCCTC GCAAACGGGC AGCGGCAGCG GCGGCAACAA CACTAGCAAT 
AGCAACAACA GCAGCAGCGG AAGCGGAGGC AGCGGCTCAG GTAGCAGCAC AGGGAACACC 
GCCAGCGGTA GCGGGAACAA CAACAGCACA AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC 
GGCAACAACG GAAACGGTAG CGGTAGCAGC TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA 
AAGATGTCCA TAGACCACCA AGCGACGCTC GACAAAGGAC TCAAAATGAA GATCAAGCGC 
ACCAAGCCGG GCACCAAAAG CTCGGAGGCC AAACACGAGA TTGTGAAGGC CACCGATCAG 
CAACAGAACG GAGCCCTGGG CGCCGGATCC AATAACTCGG CCAACGAGGA TGGGAGTTCC 
GGTTCCAGTT CCACCAACGC GTCTTCCCTG GGGAGCACCA ATTCATCGAG CAGCGCAAGC 
AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT TCGAGCAGCA GCAAGAAGCA CCTAAACAAT 
GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC GGAGGAGGGA GCCAAAACAA TGCCAGTGGC 
CATGCCAGCG GAGGGGGATC CTCCGGTAGC AGTCAGTCTA CGCCGCAGGG CACTAAGCGC 
GGTAGTTCGG GTCATCGGCG TGAGAAGACC AAGGACAAGA ACGCACATTC CAATCGCATG 
TCCGTGGACA AGTCAGCAGC TGCTGCCTCG GCGGCCGGCG AGAAGGATAC ACCTGAGAAG 
TGTTCCGGTA CGGGAGCTGG TGGATCTCCC TGCTCCTGCA ACGGAGATGT GGGAGCTCCT 
TGCTCACATC ATGCCTGCAT TCGCCGTGCC GCACACATGT CCAACTCCGC AGGCAATGCG 
AATTCGAGTG CCGGCACTGG GCAATCCGGT GGTTCATCCT CCATGTCAGC AGTGCCACCG 
GGTGTGTTCA CACCTTCAGC GGGCTCTCCT TCCACCGGTT CACCATCGAC CGTGGTGCCT 
GCCGCTGCCT CACTTCTGGC AGCGACCGGA GCAGCCTCCT CCTCCGCCTC TCAAATGGCC 
AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG GGAGGAGGTG CCAATGCACC AGGACCGCCG 
GGTAAGGAGT CTGCCGGCAG CATCAAAATC TCCTCGCACA TTGCCGCCCA ACTGGCAGCA 
GCGGCCGCTT CCAATAGTTA CAGCGGGAGT GGAGCCAACA CGAATCAAGG CCAGAACAGC 
AATGCTGGCG GCAACGGCGG TTCGGAGAGC AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA 
ATGGCACCCG GCATGATCTC AGCCACCATG CACCATACGA TCTCGGTGCC GGCGGGCACA 
GGAACAGGCG ACGACGATAC CAAATCTCCG CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC 
GCTAGTGGAG CAGGAGGCGG CAAGGAGATG GTGGACATCT GCATTGGCAC CTCGGTGGGC 
ACCATCACCG AACCGGACTG CCTGGGTCCC TGCGAACCTG GCACTTCCGT AACCCTCGAA 
GGGATTGTGT GGCACGAGAC CGAAGGCGGC GTTCTCGTGG TCAATGTCAC GTGGCGGGGC 
AAGACCTACG TGGGCACTCT GCTAGACTGC ACCCGACACG ATTGGGCTCC TCCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995883.1
CDS	2063..4882
Translation	MESMRRNEANTSHHPNKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPR

Target ORF information:

RefSeq Version	NM_206161.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206161.3

ORF Insert Sequence:

ATGGAGTCAA TGAGACGTAA CGAGGCGAAC ACTAGCCACC ACCCGAATAA GGTGGCCCTG 
AATAGCAGCA ACGTAGCAAC GGAAAGCAAT ATTAAGAATA ATAAATTGTT AGCTAATTTA 
AGTGCGGCAG GAGCAGCTAC AGCTACAACG GCAACAACAG GAACAGCAAC AACAACGACG 
GCGACAACAG CGAATCAAGC GCTGAATTTC AATAACAAAA CGAAAGCAAC AGCAAACGCG 
ACTGCAGCAT CGGCGAATAA TCGGCACAAT AACAACAACA ATTCCAGTGC GATCAAGAAG 
CACACAAATA CGAAACAACT AGCTGGCAAG AGCCCCGCGT GCAACTCCTC CTCCTCGTCG 
CTCTCCTCGT CGAGCAGCTC CAACTCGAGC GAGTCGAAGG ACACGAACTT TGAGTACGAG 
GACGAGTGGA ATATTGGCGG CATACCAGAG CTGCTGGACG ACTTGGACGC GGATATTGAG 
AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC CAGGCTACCG CGCTAAATGC CAAGCAGGCA 
AACAGCTCCT CCACATCCTC CTCATCATCC TCCAAGGGCG GCGCTTCATC CTCGTCATCC 
TCAGCAGCAG CGACGGGATC ATCATCGTCG CATAAATCGC ACAAAACCAC ATTGCACAGC 
AATTTGTCGG CCACATCGCC AACCACAATT AAGTTCACAC GCCAACCGGT GGCCATTGGC 
GGAGCTAACT CCTCCTCATC TTCATCAGCA GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC 
GTGGTGGCCA AGGGATCATC TTCTTCCTCG TCCTCCACAT CTTCCTCATC TTCGGGGAAG 
CATCATCACC ATCACCACCA TCACTCAAAC TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC 
TACAAGTCCG CTTTGGTGGC TCAACTGAAC AGTCCGAGTC CACTGAACAG TAACTCCAAG 
TCCCTGAGTG GATCGGGGAG TGGAAGCGGG AACACAAACG GAGCAGCGGG AGCTGGAGCC 
GGCAGCACAT TGTCATCCTC GACATTTGCC GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT 
TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG GCTGGCAGTG GACAACAGGG CTCCAAATTC 
TCCGCTGGCG GCATGTCCTC GCAAACGGGC AGCGGCAGCG GCGGCAACAA CACTAGCAAT 
AGCAACAACA GCAGCAGCGG AAGCGGAGGC AGCGGCTCAG GTAGCAGCAC AGGGAACACC 
GCCAGCGGTA GCGGGAACAA CAACAGCACA AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC 
GGCAACAACG GAAACGGTAG CGGTAGCAGC TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA 
AAGATGTCCA TAGACCACCA AGCGACGCTC GACAAAGGAC TCAAAATGAA GATCAAGCGC 
ACCAAGCCGG GCACCAAAAG CTCGGAGGCC AAACACGAGA TTGTGAAGGC CACCGATCAG 
CAACAGAACG GAGCCCTGGG CGCCGGATCC AATAACTCGG CCAACGAGGA TGGGAGTTCC 
GGTTCCAGTT CCACCAACGC GTCTTCCCTG GGGAGCACCA ATTCATCGAG CAGCGCAAGC 
AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT TCGAGCAGCA GCAAGAAGCA CCTAAACAAT 
GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC GGAGGAGGGA GCCAAAACAA TGCCAGTGGC 
CATGCCAGCG GAGGGGGATC CTCCGGTAGC AGTCAGTCTA CGCCGCAGGG CACTAAGCGC 
GGTAGTTCGG GTCATCGGCG TGAGAAGACC AAGGACAAGA ACGCACATTC CAATCGCATG 
TCCGTGGACA AGTCAGCAGC TGCTGCCTCG GCGGCCGGCG AGAAGGATAC ACCTGAGAAG 
TGTTCCGGTA CGGGAGCTGG TGGATCTCCC TGCTCCTGCA ACGGAGATGT GGGAGCTCCT 
TGCTCACATC ATGCCTGCAT TCGCCGTGCC GCACACATGT CCAACTCCGC AGGCAATGCG 
AATTCGAGTG CCGGCACTGG GCAATCCGGT GGTTCATCCT CCATGTCAGC AGTGCCACCG 
GGTGTGTTCA CACCTTCAGC GGGCTCTCCT TCCACCGGTT CACCATCGAC CGTGGTGCCT 
GCCGCTGCCT CACTTCTGGC AGCGACCGGA GCAGCCTCCT CCTCCGCCTC TCAAATGGCC 
AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG GGAGGAGGTG CCAATGCACC AGGACCGCCG 
GGTAAGGAGT CTGCCGGCAG CATCAAAATC TCCTCGCACA TTGCCGCCCA ACTGGCAGCA 
GCGGCCGCTT CCAATAGTTA CAGCGGGAGT GGAGCCAACA CGAATCAAGG CCAGAACAGC 
AATGCTGGCG GCAACGGCGG TTCGGAGAGC AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA 
ATGGCACCCG GCATGATCTC AGCCACCATG CACCATACGA TCTCGGTGCC GGCGGGCACA 
GGAACAGGCG ACGACGATAC CAAATCTCCG CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC 
GCTAGTGGAG CAGGAGGCGG CAAGGAGATG GTGGACATCT GCATTGGCAC CTCGGTGGGC 
ACCATCACCG AACCGGACTG CCTGGGTCCC TGCGAACCTG GCACTTCCGT AACCCTCGAA 
GGGATTGTGT GGCACGAGAC CGAAGGCGGC GTTCTCGTGG TCAATGTCAC GTGGCGGGGC 
AAGACCTACG TGGGCACTCT GCTAGACTGC ACCCGACACG ATTGGGCTCC TCCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbb ( NM_001169719.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163190.1 Drosophila melanogaster scribbler (sbb), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa11315
Clone ID Related Accession (Same CDS sequence)	NM_001169719.2 , NM_206162.4
Accession Version	NM_001169719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169719.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163190.1
CDS	2261..5050
Translation	MEKKLAKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPR

Target ORF information:

RefSeq Version	NM_001169719.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169719.2

ORF Insert Sequence:

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbb ( NM_206162.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995884.1 Drosophila melanogaster scribbler (sbb), transcript variant C, mRNA.

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CloneID	OFa11315
Clone ID Related Accession (Same CDS sequence)	NM_001169719.2 , NM_206162.4
Accession Version	NM_206162.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_206162.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995884.1
CDS	182..2971
Translation	MEKKLAKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPR

Target ORF information:

RefSeq Version	NM_206162.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206162.4

ORF Insert Sequence:

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbb ( NM_001169718.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163189.1 Drosophila melanogaster scribbler (sbb), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa11313
Clone ID Related Accession (Same CDS sequence)	NM_001169718.3 , NM_079939.5
Accession Version	NM_001169718.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001169718.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGATTC TGTGATTCAC CAACTGAAGA ATTAGATTCT 
CGAACTCCAA AGGGACGCGG AAAGCGCGGA CGCAGTGCAG GTCTCACTCC TGACCTGAGC 
AACTTCACCG AGACCAGAAG TTCGATTTAC TTCTCACACG CCCAGGTGCA CTCCAAGCTG 
CGTAATGGTG CCACAAAGGG ACGCGGTGCG ACACGCAGTG CATCCGGTAA TGCGGCAGCG 
AACAGCAACA GCAGTTCATC GGGCAACGGA GGCGGGGCCA CGCCCAGCAC ATCGCCGACG 
GCATTTTTGC CGCCACGGCC AGAGAAGCGC AAGTCGAAGG ATGAGGCGCC TTCACCGCTT 
AACGGCGACG CCTCGGATGG AGCGTCTGTT GGCGGTATTG GTGGAGCCGG TGGAGTGAAC 
ATGGTGAACG CCAGTGGCAT CCCCATATCG GCCAGCGGCG GTGGCCTGGC CACGCAGCCG 
CAGAGCCTGC TCAATCCGGT CACTGGTCTC AATGTGCAGA TCAGCACCAA GAAATGTAAG 
ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT CTGCTTGAGT GTCCCGAGCA GGACTGCAGC 
AAGAAGTACA AGCACGCAAA TGGGCTGCGT TATCACCAGT CGCATGCCCA TGGAGCGGGC 
GGTGGCGCAA GCTCCATGGA TGAGGACTCC ATGCAGGCGC CCGAGGATCC GGCCACGCCG 
CCCTCGCCAG GAGTCGCAAG TGGAACGGGA TCAGGAGCAT CTGTAGCATC ATCGGCAGTA 
CCAGCGACAG CACCATCAGC GGGTCAAGGA ACTGTCGCAG TGTCCCCAAA CACACCCCTC 
GCAAATTCAA GCAACCCCGT CACCAATGGC AATGTTGCAC CATCGGCACC AGCTACTGGA 
TCGGTAACTA TCGCCGCTCC CAACACTACT CCCTCTGTGG TAGAGACGCA AGCGCCGCTT 
ACTGGTCCTC CACCAGTCAC GCCACCAGCT CCAACTCCCA TCTGCGCAGT TGCAACACCC 
GGAGCGGAGC AATCTGTATC ATCAGTATTG CCCCTGGGAA ATCTTCCACT GACGGCAGGT 
CCAAATAGCG CGACACAACA GCAACAGCCT CCTACACAGC AGCAACAACC ACAGTTGCTC 
GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC 
GGAGGCAGCA TCACTGCTGG AATCTCCGGA CAGGCTCTGT CACAGCATCA ACAGCAACTA 
ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC CAGCAGCAGC AGGCATTGTT GCAGCAGGGA 
GCACTTAAAG CGGGTGTACT GCGTTTTGGC CCGCCAGATG GAAATCCCCT GCAGCAGCAG 
CCTGGTCAAG CATCGGTTAA TCCACAGACA CAACAGTCTC CTCCAAGGCC TCCCAGTCAT 
GTCCAGGATC AACAGACGCC ATCGGCCTAC GCCCAGCAGG CAGGTTTAAA GACATCGCCT 
GGATTTGGCT CCGTTGGCGT GGGTGCCGCT AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA 
CCTGGACCCA GTGACTTTGA GGGCCGCGTA TCGCGCGAGG ATGTACAGAG TCCGGCATAC 
AGTGATATCT CCGATGACTC CACGCCAGTT GCCGAACAGG AAATGCTGGA CAAGTCAGTC 
GGCCAGGCGG TGACGGCAAA GCACATCGAA TTGATGGGCA AGAAGCCGAC GGAAGTGGGC 
GTTGGTGTTC CGCCACCACC AGCTCCCAAT ATGTATGTGC CGGGAATGTA TCAGTTCTAT 
CCGGCGCAGC AGCAGTCAGC ACCTCCACCA CAGCAACAGC AACAACAGCC GCAATACATG 
GTGCAGACAG AGCCAGGCAA ACCACCAGGA TTGCCACCTG CTTTGACACA AGCTCAGCAG 
CAGCAACAAT TGCAGCCGGG TGCTCCTCCG CCTACCTCAC AGCCCCCGAG TCATTTGCTA 
GGTCCACCCG GTCAGCAGTC GGTGGCTGCC CACCTGGCAG ACTACAGTGG CAAGAACAAG 
GATCCACCAT TGGATCTGAT GACCAAACCG CAGCCACAGC CGGGTCAGCC GCCTTCACAG 
CAGCAGCAGT CGGGCCAACT GTCGGGGCAG GAAAACAATG GCAAGGATGT TGGACCGCCC 
ACCTCTCAGC CAGGATCTCA GCCGCCGCCG GTTAACCTAA GTGCAGTGGC TGGGCCGCCG 
CCAGGAAGCC TGCCACCCGG TTTGGGTGGT CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT 
GGTCCTGGAC CGGGCAAGGG CATGCCCCAC TTCTATCCCT TCAATTTTAT TCCGCCTGCG 
TATCCATACA ATGTGGACCC CAACTTTGGG TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC 
GCCAAGCTGA GTGGGCATCC GGGTCTGCCA CCCAGCTCAC AAGCGCAGCA GTTATCCGGC 
ATAAGTATCA AGGAGGAGCG TCTGAAGGAA AGCCCCAGTC CGCACGACCA GCCGAAGCAC 
ATGCCATCTC AGCAACAGAT GATAGCCAGC AAGCTAATCA AGCAGGAGCC GATGACCAAG 
CAGGAGATCA AGCAGGAGCC CAACTCAAAT CCGGGCCAGC AGCATCCGCC TCCGCAACAG 
CAGCCAGCGC CTCAGCCGCA GCAACAACAG CCGCCACCCC CGCAGCCACA ACAGCCGCAT 
GCCCTGCATC CCAAGGATTT ACAGGCACTG GGCGCCTATC CCGCCATCTA CCAGCGCCAT 
TCCATAAACC TGGCCGTGCA GCAGGCTCGC GAGGAGGAGC TGAGACGGTA TTACATGTTC 
ACTGGACGAC AGAATTCGGC AGCCGCAGCA GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT 
GGTGGTCTTC CACCGCATCC CGGCATGATG CACAAGGATG AGCCTGGCAT GGGATCGGCG 
GCGCAACAAC AGCAGCAGCA ACAACAGCAG CAGATGCAAA TTGCACAGCA GCAGCAGCAG 
GCCATTCAGC AGCATCATCA GCACCTGCAG CAGCAGCACC AGGCCCAGCA ACAGCAGCAG 
CAGCAACAAC ACCAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAACA ACAGCAGCAG 
CAGCAACAAC AGCAAAAGTT AAAGCAATCG CAGGCGGCTA GTGCGGGGGC AAATAATAAG 
GCCACCAACC TGACGAAGGA TTCTCCCAAA CAAAAGGGCG GCGATGACGA TCAACCGCTG 
AAGGTGAAGC AGGAGGGCCA AAAGCCGACC ATGGAGACGC AGGGTCCGCC ACCACCACCC 
ACGTCGCAGT ACTTCCTTCA CCCCTCATAC ATTTCCCCGA CGCCCTTTGG GTTTGATCCC 
AATCATCCGA TGTATCGCAA CGTGTTGATG TCAGCCGCCG GACCGTATAA TACGGCACCG 
TACCACCTGC CCATCCCTCG CCCGTACCAT GCCCCAGAAG ATCTCTCGCG TAACACCGGC 
ACCAAGGCGT TGGATGCACT CCATCACGCG GCCAGTCAGT ACTACACCAC CCACAAGATC 
CATGAGCTCA GCGAACGGGC CCTCAAGTCG CCCACCAGCG GCAGCGGCCC CGTCAAGGTG 
AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT CAACCAGGCG GACCCACGAG CAGCGGCCCC 
GGATCCGGAC CAGTTTCCGG TGTCCTCGGC CCCGGCAGCG GTTCCAACCA GCAGCCCGGC 
TCGGCACCTG GGTCTGCGGG CGGTGTGCCG CTGAACCTAC AGCCACCACC CGGAGGAATG 
GGCCCGTCAC CCGGCAGCAA GCCGGATCTG TCCGGCCCGA AAGGACATGG CGGAGTGACG 
CCCGGCTCGT CATTGGACGG CCACAAGCAG TCGATGCCCG GAGGTCCGCC ACCGAACGGG 
CCATCGGGCA ATGGAGCCGT TGGCGGTGTG GGAGGCGCTG CCGGCAATGG CGCAGCGGGA 
GGCGGCGGTG CAGGTGCCGC CGACTCACGC AGCCCACCAC CGCAGCGCCA TGTGCACACC 
CACCACCACA CGCACGTCGG CCTCGGCTAT CCCATGTACC CGGCGCCGTA TGGAGCGGCT 
GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA GCTGTGATAA ACCCGTTTCC GCCGGGTCCG 
TCGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163189.1
CDS	2366..9274
Translation	MEKKLAKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPRFCDSPTEELDSRTPKGRGKRGRSAGLTPDLSNFTETRSSIYFSHAQVHSKLRNGATKGRGATRSASGNAAANSNSSSSGNGGGATPSTSPTAFLPPRPEKRKSKDEAPSPLNGDASDGASVGGIGGAGGVNMVNASGIPISASGGGLATQPQSLLNPVTGLNVQISTKKCKTASPCAISPVLLECPEQDCSKKYKHANGLRYHQSHAHGAGGGASSMDEDSMQAPEDPATPPSPGVASGTGSGASVASSAVPATAPSAGQGTVAVSPNTPLANSSNPVTNGNVAPSAPATGSVTIAAPNTTPSVVETQAPLTGPPPVTPPAPTPICAVATPGAEQSVSSVLPLGNLPLTAGPNSATQQQQPPTQQQQPQLLVPGGSAASLQQQQQQQQPVAGGSITAGISGQALSQHQQQLMGGLPAMLSDQQQQALLQQGALKAGVLRFGPPDGNPLQQQPGQASVNPQTQQSPPRPPSHVQDQQTPSAYAQQAGLKTSPGFGSVGVGAASSKQKKNRKSPGPSDFEGRVSREDVQSPAYSDISDDSTPVAEQEMLDKSVGQAVTAKHIELMGKKPTEVGVGVPPPPAPNMYVPGMYQFYPAQQQSAPPPQQQQQQPQYMVQTEPGKPPGLPPALTQAQQQQQLQPGAPPPTSQPPSHLLGPPGQQSVAAHLADYSGKNKDPPLDLMTKPQPQPGQPPSQQQQSGQLSGQENNGKDVGPPTSQPGSQPPPVNLSAVAGPPPGSLPPGLGGLSALGAAGLGGPGPGKGMPHFYPFNFIPPAYPYNVDPNFGSVSIVASEEAAKLSGHPGLPPSSQAQQLSGISIKEERLKESPSPHDQPKHMPSQQQMIASKLIKQEPMTKQEIKQEPNSNPGQQHPPPQQQPAPQPQQQQPPPPQPQQPHALHPKDLQALGAYPAIYQRHSINLAVQQAREEELRRYYMFTGRQNSAAAAAAAAAQNAASGGLPPHPGMMHKDEPGMGSAAQQQQQQQQQQMQIAQQQQQAIQQHHQHLQQQHQAQQQQQQQQHQQQQQQQQQQQQQQQQQQQQQKLKQSQAASAGANNKATNLTKDSPKQKGGDDDQPLKVKQEGQKPTMETQGPPPPPTSQYFLHPSYISPTPFGFDPNHPMYRNVLMSAAGPYNTAPYHLPIPRPYHAPEDLSRNTGTKALDALHHAASQYYTTHKIHELSERALKSPTSGSGPVKVSVSSPSIGPPQPGGPTSSGPGSGPVSGVLGPGSGSNQQPGSAPGSAGGVPLNLQPPPGGMGPSPGSKPDLSGPKGHGGVTPGSSLDGHKQSMPGGPPPNGPSGNGAVGGVGGAAGNGAAGGGGAGAADSRSPPPQRHVHTHHHTHVGLGYPMYPAPYGAAVLASQQAAAVAVINPFPPGPSK

Target ORF information:

RefSeq Version	NM_001169718.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169718.3

ORF Insert Sequence:

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGATTC TGTGATTCAC CAACTGAAGA ATTAGATTCT 
CGAACTCCAA AGGGACGCGG AAAGCGCGGA CGCAGTGCAG GTCTCACTCC TGACCTGAGC 
AACTTCACCG AGACCAGAAG TTCGATTTAC TTCTCACACG CCCAGGTGCA CTCCAAGCTG 
CGTAATGGTG CCACAAAGGG ACGCGGTGCG ACACGCAGTG CATCCGGTAA TGCGGCAGCG 
AACAGCAACA GCAGTTCATC GGGCAACGGA GGCGGGGCCA CGCCCAGCAC ATCGCCGACG 
GCATTTTTGC CGCCACGGCC AGAGAAGCGC AAGTCGAAGG ATGAGGCGCC TTCACCGCTT 
AACGGCGACG CCTCGGATGG AGCGTCTGTT GGCGGTATTG GTGGAGCCGG TGGAGTGAAC 
ATGGTGAACG CCAGTGGCAT CCCCATATCG GCCAGCGGCG GTGGCCTGGC CACGCAGCCG 
CAGAGCCTGC TCAATCCGGT CACTGGTCTC AATGTGCAGA TCAGCACCAA GAAATGTAAG 
ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT CTGCTTGAGT GTCCCGAGCA GGACTGCAGC 
AAGAAGTACA AGCACGCAAA TGGGCTGCGT TATCACCAGT CGCATGCCCA TGGAGCGGGC 
GGTGGCGCAA GCTCCATGGA TGAGGACTCC ATGCAGGCGC CCGAGGATCC GGCCACGCCG 
CCCTCGCCAG GAGTCGCAAG TGGAACGGGA TCAGGAGCAT CTGTAGCATC ATCGGCAGTA 
CCAGCGACAG CACCATCAGC GGGTCAAGGA ACTGTCGCAG TGTCCCCAAA CACACCCCTC 
GCAAATTCAA GCAACCCCGT CACCAATGGC AATGTTGCAC CATCGGCACC AGCTACTGGA 
TCGGTAACTA TCGCCGCTCC CAACACTACT CCCTCTGTGG TAGAGACGCA AGCGCCGCTT 
ACTGGTCCTC CACCAGTCAC GCCACCAGCT CCAACTCCCA TCTGCGCAGT TGCAACACCC 
GGAGCGGAGC AATCTGTATC ATCAGTATTG CCCCTGGGAA ATCTTCCACT GACGGCAGGT 
CCAAATAGCG CGACACAACA GCAACAGCCT CCTACACAGC AGCAACAACC ACAGTTGCTC 
GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC 
GGAGGCAGCA TCACTGCTGG AATCTCCGGA CAGGCTCTGT CACAGCATCA ACAGCAACTA 
ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC CAGCAGCAGC AGGCATTGTT GCAGCAGGGA 
GCACTTAAAG CGGGTGTACT GCGTTTTGGC CCGCCAGATG GAAATCCCCT GCAGCAGCAG 
CCTGGTCAAG CATCGGTTAA TCCACAGACA CAACAGTCTC CTCCAAGGCC TCCCAGTCAT 
GTCCAGGATC AACAGACGCC ATCGGCCTAC GCCCAGCAGG CAGGTTTAAA GACATCGCCT 
GGATTTGGCT CCGTTGGCGT GGGTGCCGCT AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA 
CCTGGACCCA GTGACTTTGA GGGCCGCGTA TCGCGCGAGG ATGTACAGAG TCCGGCATAC 
AGTGATATCT CCGATGACTC CACGCCAGTT GCCGAACAGG AAATGCTGGA CAAGTCAGTC 
GGCCAGGCGG TGACGGCAAA GCACATCGAA TTGATGGGCA AGAAGCCGAC GGAAGTGGGC 
GTTGGTGTTC CGCCACCACC AGCTCCCAAT ATGTATGTGC CGGGAATGTA TCAGTTCTAT 
CCGGCGCAGC AGCAGTCAGC ACCTCCACCA CAGCAACAGC AACAACAGCC GCAATACATG 
GTGCAGACAG AGCCAGGCAA ACCACCAGGA TTGCCACCTG CTTTGACACA AGCTCAGCAG 
CAGCAACAAT TGCAGCCGGG TGCTCCTCCG CCTACCTCAC AGCCCCCGAG TCATTTGCTA 
GGTCCACCCG GTCAGCAGTC GGTGGCTGCC CACCTGGCAG ACTACAGTGG CAAGAACAAG 
GATCCACCAT TGGATCTGAT GACCAAACCG CAGCCACAGC CGGGTCAGCC GCCTTCACAG 
CAGCAGCAGT CGGGCCAACT GTCGGGGCAG GAAAACAATG GCAAGGATGT TGGACCGCCC 
ACCTCTCAGC CAGGATCTCA GCCGCCGCCG GTTAACCTAA GTGCAGTGGC TGGGCCGCCG 
CCAGGAAGCC TGCCACCCGG TTTGGGTGGT CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT 
GGTCCTGGAC CGGGCAAGGG CATGCCCCAC TTCTATCCCT TCAATTTTAT TCCGCCTGCG 
TATCCATACA ATGTGGACCC CAACTTTGGG TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC 
GCCAAGCTGA GTGGGCATCC GGGTCTGCCA CCCAGCTCAC AAGCGCAGCA GTTATCCGGC 
ATAAGTATCA AGGAGGAGCG TCTGAAGGAA AGCCCCAGTC CGCACGACCA GCCGAAGCAC 
ATGCCATCTC AGCAACAGAT GATAGCCAGC AAGCTAATCA AGCAGGAGCC GATGACCAAG 
CAGGAGATCA AGCAGGAGCC CAACTCAAAT CCGGGCCAGC AGCATCCGCC TCCGCAACAG 
CAGCCAGCGC CTCAGCCGCA GCAACAACAG CCGCCACCCC CGCAGCCACA ACAGCCGCAT 
GCCCTGCATC CCAAGGATTT ACAGGCACTG GGCGCCTATC CCGCCATCTA CCAGCGCCAT 
TCCATAAACC TGGCCGTGCA GCAGGCTCGC GAGGAGGAGC TGAGACGGTA TTACATGTTC 
ACTGGACGAC AGAATTCGGC AGCCGCAGCA GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT 
GGTGGTCTTC CACCGCATCC CGGCATGATG CACAAGGATG AGCCTGGCAT GGGATCGGCG 
GCGCAACAAC AGCAGCAGCA ACAACAGCAG CAGATGCAAA TTGCACAGCA GCAGCAGCAG 
GCCATTCAGC AGCATCATCA GCACCTGCAG CAGCAGCACC AGGCCCAGCA ACAGCAGCAG 
CAGCAACAAC ACCAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAACA ACAGCAGCAG 
CAGCAACAAC AGCAAAAGTT AAAGCAATCG CAGGCGGCTA GTGCGGGGGC AAATAATAAG 
GCCACCAACC TGACGAAGGA TTCTCCCAAA CAAAAGGGCG GCGATGACGA TCAACCGCTG 
AAGGTGAAGC AGGAGGGCCA AAAGCCGACC ATGGAGACGC AGGGTCCGCC ACCACCACCC 
ACGTCGCAGT ACTTCCTTCA CCCCTCATAC ATTTCCCCGA CGCCCTTTGG GTTTGATCCC 
AATCATCCGA TGTATCGCAA CGTGTTGATG TCAGCCGCCG GACCGTATAA TACGGCACCG 
TACCACCTGC CCATCCCTCG CCCGTACCAT GCCCCAGAAG ATCTCTCGCG TAACACCGGC 
ACCAAGGCGT TGGATGCACT CCATCACGCG GCCAGTCAGT ACTACACCAC CCACAAGATC 
CATGAGCTCA GCGAACGGGC CCTCAAGTCG CCCACCAGCG GCAGCGGCCC CGTCAAGGTG 
AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT CAACCAGGCG GACCCACGAG CAGCGGCCCC 
GGATCCGGAC CAGTTTCCGG TGTCCTCGGC CCCGGCAGCG GTTCCAACCA GCAGCCCGGC 
TCGGCACCTG GGTCTGCGGG CGGTGTGCCG CTGAACCTAC AGCCACCACC CGGAGGAATG 
GGCCCGTCAC CCGGCAGCAA GCCGGATCTG TCCGGCCCGA AAGGACATGG CGGAGTGACG 
CCCGGCTCGT CATTGGACGG CCACAAGCAG TCGATGCCCG GAGGTCCGCC ACCGAACGGG 
CCATCGGGCA ATGGAGCCGT TGGCGGTGTG GGAGGCGCTG CCGGCAATGG CGCAGCGGGA 
GGCGGCGGTG CAGGTGCCGC CGACTCACGC AGCCCACCAC CGCAGCGCCA TGTGCACACC 
CACCACCACA CGCACGTCGG CCTCGGCTAT CCCATGTACC CGGCGCCGTA TGGAGCGGCT 
GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA GCTGTGATAA ACCCGTTTCC GCCGGGTCCG 
TCGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbb ( NM_079939.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524678.3 Drosophila melanogaster scribbler (sbb), transcript variant H, mRNA.

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CloneID	OFa11313
Clone ID Related Accession (Same CDS sequence)	NM_001169718.3 , NM_079939.5
Accession Version	NM_079939.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_079939.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGATTC TGTGATTCAC CAACTGAAGA ATTAGATTCT 
CGAACTCCAA AGGGACGCGG AAAGCGCGGA CGCAGTGCAG GTCTCACTCC TGACCTGAGC 
AACTTCACCG AGACCAGAAG TTCGATTTAC TTCTCACACG CCCAGGTGCA CTCCAAGCTG 
CGTAATGGTG CCACAAAGGG ACGCGGTGCG ACACGCAGTG CATCCGGTAA TGCGGCAGCG 
AACAGCAACA GCAGTTCATC GGGCAACGGA GGCGGGGCCA CGCCCAGCAC ATCGCCGACG 
GCATTTTTGC CGCCACGGCC AGAGAAGCGC AAGTCGAAGG ATGAGGCGCC TTCACCGCTT 
AACGGCGACG CCTCGGATGG AGCGTCTGTT GGCGGTATTG GTGGAGCCGG TGGAGTGAAC 
ATGGTGAACG CCAGTGGCAT CCCCATATCG GCCAGCGGCG GTGGCCTGGC CACGCAGCCG 
CAGAGCCTGC TCAATCCGGT CACTGGTCTC AATGTGCAGA TCAGCACCAA GAAATGTAAG 
ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT CTGCTTGAGT GTCCCGAGCA GGACTGCAGC 
AAGAAGTACA AGCACGCAAA TGGGCTGCGT TATCACCAGT CGCATGCCCA TGGAGCGGGC 
GGTGGCGCAA GCTCCATGGA TGAGGACTCC ATGCAGGCGC CCGAGGATCC GGCCACGCCG 
CCCTCGCCAG GAGTCGCAAG TGGAACGGGA TCAGGAGCAT CTGTAGCATC ATCGGCAGTA 
CCAGCGACAG CACCATCAGC GGGTCAAGGA ACTGTCGCAG TGTCCCCAAA CACACCCCTC 
GCAAATTCAA GCAACCCCGT CACCAATGGC AATGTTGCAC CATCGGCACC AGCTACTGGA 
TCGGTAACTA TCGCCGCTCC CAACACTACT CCCTCTGTGG TAGAGACGCA AGCGCCGCTT 
ACTGGTCCTC CACCAGTCAC GCCACCAGCT CCAACTCCCA TCTGCGCAGT TGCAACACCC 
GGAGCGGAGC AATCTGTATC ATCAGTATTG CCCCTGGGAA ATCTTCCACT GACGGCAGGT 
CCAAATAGCG CGACACAACA GCAACAGCCT CCTACACAGC AGCAACAACC ACAGTTGCTC 
GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC 
GGAGGCAGCA TCACTGCTGG AATCTCCGGA CAGGCTCTGT CACAGCATCA ACAGCAACTA 
ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC CAGCAGCAGC AGGCATTGTT GCAGCAGGGA 
GCACTTAAAG CGGGTGTACT GCGTTTTGGC CCGCCAGATG GAAATCCCCT GCAGCAGCAG 
CCTGGTCAAG CATCGGTTAA TCCACAGACA CAACAGTCTC CTCCAAGGCC TCCCAGTCAT 
GTCCAGGATC AACAGACGCC ATCGGCCTAC GCCCAGCAGG CAGGTTTAAA GACATCGCCT 
GGATTTGGCT CCGTTGGCGT GGGTGCCGCT AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA 
CCTGGACCCA GTGACTTTGA GGGCCGCGTA TCGCGCGAGG ATGTACAGAG TCCGGCATAC 
AGTGATATCT CCGATGACTC CACGCCAGTT GCCGAACAGG AAATGCTGGA CAAGTCAGTC 
GGCCAGGCGG TGACGGCAAA GCACATCGAA TTGATGGGCA AGAAGCCGAC GGAAGTGGGC 
GTTGGTGTTC CGCCACCACC AGCTCCCAAT ATGTATGTGC CGGGAATGTA TCAGTTCTAT 
CCGGCGCAGC AGCAGTCAGC ACCTCCACCA CAGCAACAGC AACAACAGCC GCAATACATG 
GTGCAGACAG AGCCAGGCAA ACCACCAGGA TTGCCACCTG CTTTGACACA AGCTCAGCAG 
CAGCAACAAT TGCAGCCGGG TGCTCCTCCG CCTACCTCAC AGCCCCCGAG TCATTTGCTA 
GGTCCACCCG GTCAGCAGTC GGTGGCTGCC CACCTGGCAG ACTACAGTGG CAAGAACAAG 
GATCCACCAT TGGATCTGAT GACCAAACCG CAGCCACAGC CGGGTCAGCC GCCTTCACAG 
CAGCAGCAGT CGGGCCAACT GTCGGGGCAG GAAAACAATG GCAAGGATGT TGGACCGCCC 
ACCTCTCAGC CAGGATCTCA GCCGCCGCCG GTTAACCTAA GTGCAGTGGC TGGGCCGCCG 
CCAGGAAGCC TGCCACCCGG TTTGGGTGGT CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT 
GGTCCTGGAC CGGGCAAGGG CATGCCCCAC TTCTATCCCT TCAATTTTAT TCCGCCTGCG 
TATCCATACA ATGTGGACCC CAACTTTGGG TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC 
GCCAAGCTGA GTGGGCATCC GGGTCTGCCA CCCAGCTCAC AAGCGCAGCA GTTATCCGGC 
ATAAGTATCA AGGAGGAGCG TCTGAAGGAA AGCCCCAGTC CGCACGACCA GCCGAAGCAC 
ATGCCATCTC AGCAACAGAT GATAGCCAGC AAGCTAATCA AGCAGGAGCC GATGACCAAG 
CAGGAGATCA AGCAGGAGCC CAACTCAAAT CCGGGCCAGC AGCATCCGCC TCCGCAACAG 
CAGCCAGCGC CTCAGCCGCA GCAACAACAG CCGCCACCCC CGCAGCCACA ACAGCCGCAT 
GCCCTGCATC CCAAGGATTT ACAGGCACTG GGCGCCTATC CCGCCATCTA CCAGCGCCAT 
TCCATAAACC TGGCCGTGCA GCAGGCTCGC GAGGAGGAGC TGAGACGGTA TTACATGTTC 
ACTGGACGAC AGAATTCGGC AGCCGCAGCA GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT 
GGTGGTCTTC CACCGCATCC CGGCATGATG CACAAGGATG AGCCTGGCAT GGGATCGGCG 
GCGCAACAAC AGCAGCAGCA ACAACAGCAG CAGATGCAAA TTGCACAGCA GCAGCAGCAG 
GCCATTCAGC AGCATCATCA GCACCTGCAG CAGCAGCACC AGGCCCAGCA ACAGCAGCAG 
CAGCAACAAC ACCAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAACA ACAGCAGCAG 
CAGCAACAAC AGCAAAAGTT AAAGCAATCG CAGGCGGCTA GTGCGGGGGC AAATAATAAG 
GCCACCAACC TGACGAAGGA TTCTCCCAAA CAAAAGGGCG GCGATGACGA TCAACCGCTG 
AAGGTGAAGC AGGAGGGCCA AAAGCCGACC ATGGAGACGC AGGGTCCGCC ACCACCACCC 
ACGTCGCAGT ACTTCCTTCA CCCCTCATAC ATTTCCCCGA CGCCCTTTGG GTTTGATCCC 
AATCATCCGA TGTATCGCAA CGTGTTGATG TCAGCCGCCG GACCGTATAA TACGGCACCG 
TACCACCTGC CCATCCCTCG CCCGTACCAT GCCCCAGAAG ATCTCTCGCG TAACACCGGC 
ACCAAGGCGT TGGATGCACT CCATCACGCG GCCAGTCAGT ACTACACCAC CCACAAGATC 
CATGAGCTCA GCGAACGGGC CCTCAAGTCG CCCACCAGCG GCAGCGGCCC CGTCAAGGTG 
AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT CAACCAGGCG GACCCACGAG CAGCGGCCCC 
GGATCCGGAC CAGTTTCCGG TGTCCTCGGC CCCGGCAGCG GTTCCAACCA GCAGCCCGGC 
TCGGCACCTG GGTCTGCGGG CGGTGTGCCG CTGAACCTAC AGCCACCACC CGGAGGAATG 
GGCCCGTCAC CCGGCAGCAA GCCGGATCTG TCCGGCCCGA AAGGACATGG CGGAGTGACG 
CCCGGCTCGT CATTGGACGG CCACAAGCAG TCGATGCCCG GAGGTCCGCC ACCGAACGGG 
CCATCGGGCA ATGGAGCCGT TGGCGGTGTG GGAGGCGCTG CCGGCAATGG CGCAGCGGGA 
GGCGGCGGTG CAGGTGCCGC CGACTCACGC AGCCCACCAC CGCAGCGCCA TGTGCACACC 
CACCACCACA CGCACGTCGG CCTCGGCTAT CCCATGTACC CGGCGCCGTA TGGAGCGGCT 
GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA GCTGTGATAA ACCCGTTTCC GCCGGGTCCG 
TCGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524678.3
CDS	182..7090
Translation	MEKKLAKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPRFCDSPTEELDSRTPKGRGKRGRSAGLTPDLSNFTETRSSIYFSHAQVHSKLRNGATKGRGATRSASGNAAANSNSSSSGNGGGATPSTSPTAFLPPRPEKRKSKDEAPSPLNGDASDGASVGGIGGAGGVNMVNASGIPISASGGGLATQPQSLLNPVTGLNVQISTKKCKTASPCAISPVLLECPEQDCSKKYKHANGLRYHQSHAHGAGGGASSMDEDSMQAPEDPATPPSPGVASGTGSGASVASSAVPATAPSAGQGTVAVSPNTPLANSSNPVTNGNVAPSAPATGSVTIAAPNTTPSVVETQAPLTGPPPVTPPAPTPICAVATPGAEQSVSSVLPLGNLPLTAGPNSATQQQQPPTQQQQPQLLVPGGSAASLQQQQQQQQPVAGGSITAGISGQALSQHQQQLMGGLPAMLSDQQQQALLQQGALKAGVLRFGPPDGNPLQQQPGQASVNPQTQQSPPRPPSHVQDQQTPSAYAQQAGLKTSPGFGSVGVGAASSKQKKNRKSPGPSDFEGRVSREDVQSPAYSDISDDSTPVAEQEMLDKSVGQAVTAKHIELMGKKPTEVGVGVPPPPAPNMYVPGMYQFYPAQQQSAPPPQQQQQQPQYMVQTEPGKPPGLPPALTQAQQQQQLQPGAPPPTSQPPSHLLGPPGQQSVAAHLADYSGKNKDPPLDLMTKPQPQPGQPPSQQQQSGQLSGQENNGKDVGPPTSQPGSQPPPVNLSAVAGPPPGSLPPGLGGLSALGAAGLGGPGPGKGMPHFYPFNFIPPAYPYNVDPNFGSVSIVASEEAAKLSGHPGLPPSSQAQQLSGISIKEERLKESPSPHDQPKHMPSQQQMIASKLIKQEPMTKQEIKQEPNSNPGQQHPPPQQQPAPQPQQQQPPPPQPQQPHALHPKDLQALGAYPAIYQRHSINLAVQQAREEELRRYYMFTGRQNSAAAAAAAAAQNAASGGLPPHPGMMHKDEPGMGSAAQQQQQQQQQQMQIAQQQQQAIQQHHQHLQQQHQAQQQQQQQQHQQQQQQQQQQQQQQQQQQQQQKLKQSQAASAGANNKATNLTKDSPKQKGGDDDQPLKVKQEGQKPTMETQGPPPPPTSQYFLHPSYISPTPFGFDPNHPMYRNVLMSAAGPYNTAPYHLPIPRPYHAPEDLSRNTGTKALDALHHAASQYYTTHKIHELSERALKSPTSGSGPVKVSVSSPSIGPPQPGGPTSSGPGSGPVSGVLGPGSGSNQQPGSAPGSAGGVPLNLQPPPGGMGPSPGSKPDLSGPKGHGGVTPGSSLDGHKQSMPGGPPPNGPSGNGAVGGVGGAAGNGAAGGGGAGAADSRSPPPQRHVHTHHHTHVGLGYPMYPAPYGAAVLASQQAAAVAVINPFPPGPSK

Target ORF information:

RefSeq Version	NM_079939.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079939.5

ORF Insert Sequence:

ATGGAAAAGA AGTTGGCAAA GGTGGCCCTG AATAGCAGCA ACGTAGCAAC GGAAAGCAAT 
ATTAAGAATA ATAAATTGTT AGCTAATTTA AGTGCGGCAG GAGCAGCTAC AGCTACAACG 
GCAACAACAG GAACAGCAAC AACAACGACG GCGACAACAG CGAATCAAGC GCTGAATTTC 
AATAACAAAA CGAAAGCAAC AGCAAACGCG ACTGCAGCAT CGGCGAATAA TCGGCACAAT 
AACAACAACA ATTCCAGTGC GATCAAGAAG CACACAAATA CGAAACAACT AGCTGGCAAG 
AGCCCCGCGT GCAACTCCTC CTCCTCGTCG CTCTCCTCGT CGAGCAGCTC CAACTCGAGC 
GAGTCGAAGG ACACGAACTT TGAGTACGAG GACGAGTGGA ATATTGGCGG CATACCAGAG 
CTGCTGGACG ACTTGGACGC GGATATTGAG AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC 
CAGGCTACCG CGCTAAATGC CAAGCAGGCA AACAGCTCCT CCACATCCTC CTCATCATCC 
TCCAAGGGCG GCGCTTCATC CTCGTCATCC TCAGCAGCAG CGACGGGATC ATCATCGTCG 
CATAAATCGC ACAAAACCAC ATTGCACAGC AATTTGTCGG CCACATCGCC AACCACAATT 
AAGTTCACAC GCCAACCGGT GGCCATTGGC GGAGCTAACT CCTCCTCATC TTCATCAGCA 
GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC GTGGTGGCCA AGGGATCATC TTCTTCCTCG 
TCCTCCACAT CTTCCTCATC TTCGGGGAAG CATCATCACC ATCACCACCA TCACTCAAAC 
TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC TACAAGTCCG CTTTGGTGGC TCAACTGAAC 
AGTCCGAGTC CACTGAACAG TAACTCCAAG TCCCTGAGTG GATCGGGGAG TGGAAGCGGG 
AACACAAACG GAGCAGCGGG AGCTGGAGCC GGCAGCACAT TGTCATCCTC GACATTTGCC 
GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG 
GCTGGCAGTG GACAACAGGG CTCCAAATTC TCCGCTGGCG GCATGTCCTC GCAAACGGGC 
AGCGGCAGCG GCGGCAACAA CACTAGCAAT AGCAACAACA GCAGCAGCGG AAGCGGAGGC 
AGCGGCTCAG GTAGCAGCAC AGGGAACACC GCCAGCGGTA GCGGGAACAA CAACAGCACA 
AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC GGCAACAACG GAAACGGTAG CGGTAGCAGC 
TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA AAGATGTCCA TAGACCACCA AGCGACGCTC 
GACAAAGGAC TCAAAATGAA GATCAAGCGC ACCAAGCCGG GCACCAAAAG CTCGGAGGCC 
AAACACGAGA TTGTGAAGGC CACCGATCAG CAACAGAACG GAGCCCTGGG CGCCGGATCC 
AATAACTCGG CCAACGAGGA TGGGAGTTCC GGTTCCAGTT CCACCAACGC GTCTTCCCTG 
GGGAGCACCA ATTCATCGAG CAGCGCAAGC AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT 
TCGAGCAGCA GCAAGAAGCA CCTAAACAAT GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC 
GGAGGAGGGA GCCAAAACAA TGCCAGTGGC CATGCCAGCG GAGGGGGATC CTCCGGTAGC 
AGTCAGTCTA CGCCGCAGGG CACTAAGCGC GGTAGTTCGG GTCATCGGCG TGAGAAGACC 
AAGGACAAGA ACGCACATTC CAATCGCATG TCCGTGGACA AGTCAGCAGC TGCTGCCTCG 
GCGGCCGGCG AGAAGGATAC ACCTGAGAAG TGTTCCGGTA CGGGAGCTGG TGGATCTCCC 
TGCTCCTGCA ACGGAGATGT GGGAGCTCCT TGCTCACATC ATGCCTGCAT TCGCCGTGCC 
GCACACATGT CCAACTCCGC AGGCAATGCG AATTCGAGTG CCGGCACTGG GCAATCCGGT 
GGTTCATCCT CCATGTCAGC AGTGCCACCG GGTGTGTTCA CACCTTCAGC GGGCTCTCCT 
TCCACCGGTT CACCATCGAC CGTGGTGCCT GCCGCTGCCT CACTTCTGGC AGCGACCGGA 
GCAGCCTCCT CCTCCGCCTC TCAAATGGCC AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG 
GGAGGAGGTG CCAATGCACC AGGACCGCCG GGTAAGGAGT CTGCCGGCAG CATCAAAATC 
TCCTCGCACA TTGCCGCCCA ACTGGCAGCA GCGGCCGCTT CCAATAGTTA CAGCGGGAGT 
GGAGCCAACA CGAATCAAGG CCAGAACAGC AATGCTGGCG GCAACGGCGG TTCGGAGAGC 
AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA ATGGCACCCG GCATGATCTC AGCCACCATG 
CACCATACGA TCTCGGTGCC GGCGGGCACA GGAACAGGCG ACGACGATAC CAAATCTCCG 
CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC GCTAGTGGAG CAGGAGGCGG CAAGGAGATG 
GTGGACATCT GCATTGGCAC CTCGGTGGGC ACCATCACCG AACCGGACTG CCTGGGTCCC 
TGCGAACCTG GCACTTCCGT AACCCTCGAA GGGATTGTGT GGCACGAGAC CGAAGGCGGC 
GTTCTCGTGG TCAATGTCAC GTGGCGGGGC AAGACCTACG TGGGCACTCT GCTAGACTGC 
ACCCGACACG ATTGGGCTCC TCCAAGATTC TGTGATTCAC CAACTGAAGA ATTAGATTCT 
CGAACTCCAA AGGGACGCGG AAAGCGCGGA CGCAGTGCAG GTCTCACTCC TGACCTGAGC 
AACTTCACCG AGACCAGAAG TTCGATTTAC TTCTCACACG CCCAGGTGCA CTCCAAGCTG 
CGTAATGGTG CCACAAAGGG ACGCGGTGCG ACACGCAGTG CATCCGGTAA TGCGGCAGCG 
AACAGCAACA GCAGTTCATC GGGCAACGGA GGCGGGGCCA CGCCCAGCAC ATCGCCGACG 
GCATTTTTGC CGCCACGGCC AGAGAAGCGC AAGTCGAAGG ATGAGGCGCC TTCACCGCTT 
AACGGCGACG CCTCGGATGG AGCGTCTGTT GGCGGTATTG GTGGAGCCGG TGGAGTGAAC 
ATGGTGAACG CCAGTGGCAT CCCCATATCG GCCAGCGGCG GTGGCCTGGC CACGCAGCCG 
CAGAGCCTGC TCAATCCGGT CACTGGTCTC AATGTGCAGA TCAGCACCAA GAAATGTAAG 
ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT CTGCTTGAGT GTCCCGAGCA GGACTGCAGC 
AAGAAGTACA AGCACGCAAA TGGGCTGCGT TATCACCAGT CGCATGCCCA TGGAGCGGGC 
GGTGGCGCAA GCTCCATGGA TGAGGACTCC ATGCAGGCGC CCGAGGATCC GGCCACGCCG 
CCCTCGCCAG GAGTCGCAAG TGGAACGGGA TCAGGAGCAT CTGTAGCATC ATCGGCAGTA 
CCAGCGACAG CACCATCAGC GGGTCAAGGA ACTGTCGCAG TGTCCCCAAA CACACCCCTC 
GCAAATTCAA GCAACCCCGT CACCAATGGC AATGTTGCAC CATCGGCACC AGCTACTGGA 
TCGGTAACTA TCGCCGCTCC CAACACTACT CCCTCTGTGG TAGAGACGCA AGCGCCGCTT 
ACTGGTCCTC CACCAGTCAC GCCACCAGCT CCAACTCCCA TCTGCGCAGT TGCAACACCC 
GGAGCGGAGC AATCTGTATC ATCAGTATTG CCCCTGGGAA ATCTTCCACT GACGGCAGGT 
CCAAATAGCG CGACACAACA GCAACAGCCT CCTACACAGC AGCAACAACC ACAGTTGCTC 
GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC 
GGAGGCAGCA TCACTGCTGG AATCTCCGGA CAGGCTCTGT CACAGCATCA ACAGCAACTA 
ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC CAGCAGCAGC AGGCATTGTT GCAGCAGGGA 
GCACTTAAAG CGGGTGTACT GCGTTTTGGC CCGCCAGATG GAAATCCCCT GCAGCAGCAG 
CCTGGTCAAG CATCGGTTAA TCCACAGACA CAACAGTCTC CTCCAAGGCC TCCCAGTCAT 
GTCCAGGATC AACAGACGCC ATCGGCCTAC GCCCAGCAGG CAGGTTTAAA GACATCGCCT 
GGATTTGGCT CCGTTGGCGT GGGTGCCGCT AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA 
CCTGGACCCA GTGACTTTGA GGGCCGCGTA TCGCGCGAGG ATGTACAGAG TCCGGCATAC 
AGTGATATCT CCGATGACTC CACGCCAGTT GCCGAACAGG AAATGCTGGA CAAGTCAGTC 
GGCCAGGCGG TGACGGCAAA GCACATCGAA TTGATGGGCA AGAAGCCGAC GGAAGTGGGC 
GTTGGTGTTC CGCCACCACC AGCTCCCAAT ATGTATGTGC CGGGAATGTA TCAGTTCTAT 
CCGGCGCAGC AGCAGTCAGC ACCTCCACCA CAGCAACAGC AACAACAGCC GCAATACATG 
GTGCAGACAG AGCCAGGCAA ACCACCAGGA TTGCCACCTG CTTTGACACA AGCTCAGCAG 
CAGCAACAAT TGCAGCCGGG TGCTCCTCCG CCTACCTCAC AGCCCCCGAG TCATTTGCTA 
GGTCCACCCG GTCAGCAGTC GGTGGCTGCC CACCTGGCAG ACTACAGTGG CAAGAACAAG 
GATCCACCAT TGGATCTGAT GACCAAACCG CAGCCACAGC CGGGTCAGCC GCCTTCACAG 
CAGCAGCAGT CGGGCCAACT GTCGGGGCAG GAAAACAATG GCAAGGATGT TGGACCGCCC 
ACCTCTCAGC CAGGATCTCA GCCGCCGCCG GTTAACCTAA GTGCAGTGGC TGGGCCGCCG 
CCAGGAAGCC TGCCACCCGG TTTGGGTGGT CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT 
GGTCCTGGAC CGGGCAAGGG CATGCCCCAC TTCTATCCCT TCAATTTTAT TCCGCCTGCG 
TATCCATACA ATGTGGACCC CAACTTTGGG TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC 
GCCAAGCTGA GTGGGCATCC GGGTCTGCCA CCCAGCTCAC AAGCGCAGCA GTTATCCGGC 
ATAAGTATCA AGGAGGAGCG TCTGAAGGAA AGCCCCAGTC CGCACGACCA GCCGAAGCAC 
ATGCCATCTC AGCAACAGAT GATAGCCAGC AAGCTAATCA AGCAGGAGCC GATGACCAAG 
CAGGAGATCA AGCAGGAGCC CAACTCAAAT CCGGGCCAGC AGCATCCGCC TCCGCAACAG 
CAGCCAGCGC CTCAGCCGCA GCAACAACAG CCGCCACCCC CGCAGCCACA ACAGCCGCAT 
GCCCTGCATC CCAAGGATTT ACAGGCACTG GGCGCCTATC CCGCCATCTA CCAGCGCCAT 
TCCATAAACC TGGCCGTGCA GCAGGCTCGC GAGGAGGAGC TGAGACGGTA TTACATGTTC 
ACTGGACGAC AGAATTCGGC AGCCGCAGCA GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT 
GGTGGTCTTC CACCGCATCC CGGCATGATG CACAAGGATG AGCCTGGCAT GGGATCGGCG 
GCGCAACAAC AGCAGCAGCA ACAACAGCAG CAGATGCAAA TTGCACAGCA GCAGCAGCAG 
GCCATTCAGC AGCATCATCA GCACCTGCAG CAGCAGCACC AGGCCCAGCA ACAGCAGCAG 
CAGCAACAAC ACCAGCAGCA GCAGCAACAA CAGCAGCAGC AGCAACAACA ACAGCAGCAG 
CAGCAACAAC AGCAAAAGTT AAAGCAATCG CAGGCGGCTA GTGCGGGGGC AAATAATAAG 
GCCACCAACC TGACGAAGGA TTCTCCCAAA CAAAAGGGCG GCGATGACGA TCAACCGCTG 
AAGGTGAAGC AGGAGGGCCA AAAGCCGACC ATGGAGACGC AGGGTCCGCC ACCACCACCC 
ACGTCGCAGT ACTTCCTTCA CCCCTCATAC ATTTCCCCGA CGCCCTTTGG GTTTGATCCC 
AATCATCCGA TGTATCGCAA CGTGTTGATG TCAGCCGCCG GACCGTATAA TACGGCACCG 
TACCACCTGC CCATCCCTCG CCCGTACCAT GCCCCAGAAG ATCTCTCGCG TAACACCGGC 
ACCAAGGCGT TGGATGCACT CCATCACGCG GCCAGTCAGT ACTACACCAC CCACAAGATC 
CATGAGCTCA GCGAACGGGC CCTCAAGTCG CCCACCAGCG GCAGCGGCCC CGTCAAGGTG 
AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT CAACCAGGCG GACCCACGAG CAGCGGCCCC 
GGATCCGGAC CAGTTTCCGG TGTCCTCGGC CCCGGCAGCG GTTCCAACCA GCAGCCCGGC 
TCGGCACCTG GGTCTGCGGG CGGTGTGCCG CTGAACCTAC AGCCACCACC CGGAGGAATG 
GGCCCGTCAC CCGGCAGCAA GCCGGATCTG TCCGGCCCGA AAGGACATGG CGGAGTGACG 
CCCGGCTCGT CATTGGACGG CCACAAGCAG TCGATGCCCG GAGGTCCGCC ACCGAACGGG 
CCATCGGGCA ATGGAGCCGT TGGCGGTGTG GGAGGCGCTG CCGGCAATGG CGCAGCGGGA 
GGCGGCGGTG CAGGTGCCGC CGACTCACGC AGCCCACCAC CGCAGCGCCA TGTGCACACC 
CACCACCACA CGCACGTCGG CCTCGGCTAT CCCATGTACC CGGCGCCGTA TGGAGCGGCT 
GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA GCTGTGATAA ACCCGTTTCC GCCGGGTCCG 
TCGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbb ( NM_001169720.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163191.1 Drosophila melanogaster scribbler (sbb), transcript variant G, mRNA.

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CloneID	OFa12207
Clone ID Related Accession (Same CDS sequence)	NM_001169720.2
Accession Version	NM_001169720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001169720.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAA TGAGACGTAA CGAGGCGAAC ACTAGCCACC ACCCGAATAA GGTGGCCCTG 
AATAGCAGCA ACGTAGCAAC GGAAAGCAAT ATTAAGAATA ATAAATTGTT AGCTAATTTA 
AGTGCGGCAG GAGCAGCTAC AGCTACAACG GCAACAACAG GAACAGCAAC AACAACGACG 
GCGACAACAG CGAATCAAGC GCTGAATTTC AATAACAAAA CGAAAGCAAC AGCAAACGCG 
ACTGCAGCAT CGGCGAATAA TCGGCACAAT AACAACAACA ATTCCAGTGC GATCAAGAAG 
CACACAAATA CGAAACAACT AGCTGGCAAG AGCCCCGCGT GCAACTCCTC CTCCTCGTCG 
CTCTCCTCGT CGAGCAGCTC CAACTCGAGC GAGTCGAAGG ACACGAACTT TGAGTACGAG 
GACGAGTGGA ATATTGGCGG CATACCAGAG CTGCTGGACG ACTTGGACGC GGATATTGAG 
AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC CAGGCTACCG CGCTAAATGC CAAGCAGGCA 
AACAGCTCCT CCACATCCTC CTCATCATCC TCCAAGGGCG GCGCTTCATC CTCGTCATCC 
TCAGCAGCAG CGACGGGATC ATCATCGTCG CATAAATCGC ACAAAACCAC ATTGCACAGC 
AATTTGTCGG CCACATCGCC AACCACAATT AAGTTCACAC GCCAACCGGT GGCCATTGGC 
GGAGCTAACT CCTCCTCATC TTCATCAGCA GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC 
GTGGTGGCCA AGGGATCATC TTCTTCCTCG TCCTCCACAT CTTCCTCATC TTCGGGGAAG 
CATCATCACC ATCACCACCA TCACTCAAAC TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC 
TACAAGTCCG CTTTGGTGGC TCAACTGAAC AGTCCGAGTC CACTGAACAG TAACTCCAAG 
TCCCTGAGTG GATCGGGGAG TGGAAGCGGG AACACAAACG GAGCAGCGGG AGCTGGAGCC 
GGCAGCACAT TGTCATCCTC GACATTTGCC GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT 
TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG GCTGGCAGTG GACAACAGGG CTCCAAATTC 
TCCGCTGGCG GCATGTCCTC GCAAACGGGC AGCGGCAGCG GCGGCAACAA CACTAGCAAT 
AGCAACAACA GCAGCAGCGG AAGCGGAGGC AGCGGCTCAG GTAGCAGCAC AGGGAACACC 
GCCAGCGGTA GCGGGAACAA CAACAGCACA AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC 
GGCAACAACG GAAACGGTAG CGGTAGCAGC TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA 
AAGATGTCCA TAGACCACCA AGCGACGCTC GACAAAGGAC TCAAAATGAA GATCAAGCGC 
ACCAAGCCGG GCACCAAAAG CTCGGAGGCC AAACACGAGA TTGTGAAGGC CACCGATCAG 
CAACAGAACG GAGCCCTGGG CGCCGGATCC AATAACTCGG CCAACGAGGA TGGGAGTTCC 
GGTTCCAGTT CCACCAACGC GTCTTCCCTG GGGAGCACCA ATTCATCGAG CAGCGCAAGC 
AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT TCGAGCAGCA GCAAGAAGCA CCTAAACAAT 
GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC GGAGGAGGGA GCCAAAACAA TGCCAGTGGC 
CATGCCAGCG GAGGGGGATC CTCCGGTAGC AGTCAGTCTA CGCCGCAGGG CACTAAGCGC 
GGTAGTTCGG GTCATCGGCG TGAGAAGACC AAGGACAAGA ACGCACATTC CAATCGCATG 
TCCGTGGACA AGTCAGCAGC TGCTGCCTCG GCGGCCGGCG AGAAGGATAC ACCTGAGAAG 
TGTTCCGGTA CGGGAGCTGG TGGATCTCCC TGCTCCTGCA ACGGAGATGT GGGAGCTCCT 
TGCTCACATC ATGCCTGCAT TCGCCGTGCC GCACACATGT CCAACTCCGC AGGCAATGCG 
AATTCGAGTG CCGGCACTGG GCAATCCGGT GGTTCATCCT CCATGTCAGC AGTGCCACCG 
GGTGTGTTCA CACCTTCAGC GGGCTCTCCT TCCACCGGTT CACCATCGAC CGTGGTGCCT 
GCCGCTGCCT CACTTCTGGC AGCGACCGGA GCAGCCTCCT CCTCCGCCTC TCAAATGGCC 
AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG GGAGGAGGTG CCAATGCACC AGGACCGCCG 
GGTAAGGAGT CTGCCGGCAG CATCAAAATC TCCTCGCACA TTGCCGCCCA ACTGGCAGCA 
GCGGCCGCTT CCAATAGTTA CAGCGGGAGT GGAGCCAACA CGAATCAAGG CCAGAACAGC 
AATGCTGGCG GCAACGGCGG TTCGGAGAGC AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA 
ATGGCACCCG GCATGATCTC AGCCACCATG CACCATACGA TCTCGGTGCC GGCGGGCACA 
GGAACAGGCG ACGACGATAC CAAATCTCCG CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC 
GCTAGTGGAG CAGGAGGCGG CAAGGAGATG GTGGACATCT GCATTGGCAC CTCGGTGGGC 
ACCATCACCG AACCGGACTG CCTGGGTCCC TGCGAACCTG GCACTTCCGT AACCCTCGAA 
GGGATTGTGT GGCACGAGAC CGAAGGCGGC GTTCTCGTGG TCAATGTCAC GTGGCGGGGC 
AAGACCTACG TGGGCACTCT GCTAGACTGC ACCCGACACG ATTGGGCTCC TCCAAGATTC 
TGTGATTCAC CAACTGAAGA ATTAGATTCT CGAACTCCAA AGGGACGCGG AAAGCGCGGA 
CGCAGTGCAG GTCTCACTCC TGACCTGAGC AACTTCACCG AGACCAGAAG TTCGATTTAC 
TTCTCACACG CCCAGGTGCA CTCCAAGCTG CGTAATGGTG CCACAAAGGG ACGCGGTGCG 
ACACGCAGTG CATCCGGTAA TGCGGCAGCG AACAGCAACA GCAGTTCATC GGGCAACGGA 
GGCGGGGCCA CGCCCAGCAC ATCGCCGACG GCATTTTTGC CGCCACGGCC AGAGAAGCGC 
AAGTCGAAGG ATGAGGCGCC TTCACCGCTT AACGGCGACG CCTCGGATGG AGCGTCTGTT 
GGCGGTATTG GTGGAGCCGG TGGAGTGAAC ATGGTGAACG CCAGTGGCAT CCCCATATCG 
GCCAGCGGCG GTGGCCTGGC CACGCAGCCG CAGAGCCTGC TCAATCCGGT CACTGGTCTC 
AATGTGCAGA TCAGCACCAA GAAATGTAAG ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT 
CTGCTTGAGT GTCCCGAGCA GGACTGCAGC AAGAAGTACA AGCACGCAAA TGGGCTGCGT 
TATCACCAGT CGCATGCCCA TGGAGCGGGC GGTGGCGCAA GCTCCATGGA TGAGGACTCC 
ATGCAGGCGC CCGAGGATCC GGCCACGCCG CCCTCGCCAG GAGTCGCAAG TGGAACGGGA 
TCAGGAGCAT CTGTAGCATC ATCGGCAGTA CCAGCGACAG CACCATCAGC GGGTCAAGGA 
ACTGTCGCAG TGTCCCCAAA CACACCCCTC GCAAATTCAA GCAACCCCGT CACCAATGGC 
AATGTTGCAC CATCGGCACC AGCTACTGGA TCGGTAACTA TCGCCGCTCC CAACACTACT 
CCCTCTGTGG TAGAGACGCA AGCGCCGCTT ACTGGTCCTC CACCAGTCAC GCCACCAGCT 
CCAACTCCCA TCTGCGCAGT TGCAACACCC GGAGCGGAGC AATCTGTATC ATCAGTATTG 
CCCCTGGGAA ATCTTCCACT GACGGCAGGT CCAAATAGCG CGACACAACA GCAACAGCCT 
CCTACACAGC AGCAACAACC ACAGTTGCTC GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG 
CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC GGAGGCAGCA TCACTGCTGG AATCTCCGGA 
CAGGCTCTGT CACAGCATCA ACAGCAACTA ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC 
CAGCAGCAGC AGGCATTGTT GCAGCAGGGA GCACTTAAAG CGGGTGTACT GCGTTTTGGC 
CCGCCAGATG GAAATCCCCT GCAGCAGCAG CCTGGTCAAG CATCGGTTAA TCCACAGACA 
CAACAGTCTC CTCCAAGGCC TCCCAGTCAT GTCCAGGATC AACAGACGCC ATCGGCCTAC 
GCCCAGCAGG CAGGTTTAAA GACATCGCCT GGATTTGGCT CCGTTGGCGT GGGTGCCGCT 
AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA CCTGGACCCA GTGACTTTGA GGGCCGCGTA 
TCGCGCGAGG ATGTACAGAG TCCGGCATAC AGTGATATCT CCGATGACTC CACGCCAGTT 
GCCGAACAGG AAATGCTGGA CAAGTCAGTC GGCCAGGCGG TGACGGCAAA GCACATCGAA 
TTGATGGGCA AGAAGCCGAC GGAAGTGGGC GTTGGTGTTC CGCCACCACC AGCTCCCAAT 
ATGTATGTGC CGGGAATGTA TCAGTTCTAT CCGGCGCAGC AGCAGTCAGC ACCTCCACCA 
CAGCAACAGC AACAACAGCC GCAATACATG GTGCAGACAG AGCCAGGCAA ACCACCAGGA 
TTGCCACCTG CTTTGACACA AGCTCAGCAG CAGCAACAAT TGCAGCCGGG TGCTCCTCCG 
CCTACCTCAC AGCCCCCGAG TCATTTGCTA GGTCCACCCG GTCAGCAGTC GGTGGCTGCC 
CACCTGGCAG ACTACAGTGG CAAGAACAAG GATCCACCAT TGGATCTGAT GACCAAACCG 
CAGCCACAGC CGGGTCAGCC GCCTTCACAG CAGCAGCAGT CGGGCCAACT GTCGGGGCAG 
GAAAACAATG GCAAGGATGT TGGACCGCCC ACCTCTCAGC CAGGATCTCA GCCGCCGCCG 
GTTAACCTAA GTGCAGTGGC TGGGCCGCCG CCAGGAAGCC TGCCACCCGG TTTGGGTGGT 
CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT GGTCCTGGAC CGGGCAAGGG CATGCCCCAC 
TTCTATCCCT TCAATTTTAT TCCGCCTGCG TATCCATACA ATGTGGACCC CAACTTTGGG 
TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC GCCAAGCTGA GTGGGCATCC GGGTCTGCCA 
CCCAGCTCAC AAGCGCAGCA GTTATCCGGC ATAAGTATCA AGGAGGAGCG TCTGAAGGAA 
AGCCCCAGTC CGCACGACCA GCCGAAGCAC ATGCCATCTC AGCAACAGAT GATAGCCAGC 
AAGCTAATCA AGCAGGAGCC GATGACCAAG CAGGAGATCA AGCAGGAGCC CAACTCAAAT 
CCGGGCCAGC AGCATCCGCC TCCGCAACAG CAGCCAGCGC CTCAGCCGCA GCAACAACAG 
CCGCCACCCC CGCAGCCACA ACAGCCGCAT GCCCTGCATC CCAAGGATTT ACAGGCACTG 
GGCGCCTATC CCGCCATCTA CCAGCGCCAT TCCATAAACC TGGCCGTGCA GCAGGCTCGC 
GAGGAGGAGC TGAGACGGTA TTACATGTTC ACTGGACGAC AGAATTCGGC AGCCGCAGCA 
GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT GGTGGTCTTC CACCGCATCC CGGCATGATG 
CACAAGGATG AGCCTGGCAT GGGATCGGCG GCGCAACAAC AGCAGCAGCA ACAACAGCAG 
CAGATGCAAA TTGCACAGCA GCAGCAGCAG GCCATTCAGC AGCATCATCA GCACCTGCAG 
CAGCAGCACC AGGCCCAGCA ACAGCAGCAG CAGCAACAAC ACCAGCAGCA GCAGCAACAA 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAACAAC AGCAAAAGTT AAAGCAATCG 
CAGGCGGCTA GTGCGGGGGC AAATAATAAG GCCACCAACC TGACGAAGGA TTCTCCCAAA 
CAAAAGGGCG GCGATGACGA TCAACCGCTG AAGGTGAAGC AGGAGGGCCA AAAGCCGACC 
ATGGAGACGC AGGGTCCGCC ACCACCACCC ACGTCGCAGT ACTTCCTTCA CCCCTCATAC 
ATTTCCCCGA CGCCCTTTGG GTTTGATCCC AATCATCCGA TGTATCGCAA CGTGTTGATG 
TCAGCCGCCG GACCGTATAA TACGGCACCG TACCACCTGC CCATCCCTCG CCCGTACCAT 
GCCCCAGAAG ATCTCTCGCG TAACACCGGC ACCAAGGCGT TGGATGCACT CCATCACGCG 
GCCAGTCAGT ACTACACCAC CCACAAGATC CATGAGCTCA GCGAACGGGC CCTCAAGTCG 
CCCACCAGCG GCAGCGGCCC CGTCAAGGTG AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT 
CAACCAGGCG GACCCACGAG CAGCGGCCCC GGATCCGGAC CAGTTTCCGG TGTCCTCGGC 
CCCGGCAGCG GTTCCAACCA GCAGCCCGGC TCGGCACCTG GGTCTGCGGG CGGTGTGCCG 
CTGAACCTAC AGCCACCACC CGGAGGAATG GGCCCGTCAC CCGGCAGCAA GCCGGATCTG 
TCCGGCCCGA AAGGACATGG CGGAGTGACG CCCGGCTCGT CATTGGACGG CCACAAGCAG 
TCGATGCCCG GAGGTCCGCC ACCGAACGGG CCATCGGGCA ATGGAGCCGT TGGCGGTGTG 
GGAGGCGCTG CCGGCAATGG CGCAGCGGGA GGCGGCGGTG CAGGTGCCGC CGACTCACGC 
AGCCCACCAC CGCAGCGCCA TGTGCACACC CACCACCACA CGCACGTCGG CCTCGGCTAT 
CCCATGTACC CGGCGCCGTA TGGAGCGGCT GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA 
GCTGTGATAA ACCCGTTTCC GCCGGGTCCG TCGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163191.1
CDS	981..7919
Translation	MESMRRNEANTSHHPNKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPRFCDSPTEELDSRTPKGRGKRGRSAGLTPDLSNFTETRSSIYFSHAQVHSKLRNGATKGRGATRSASGNAAANSNSSSSGNGGGATPSTSPTAFLPPRPEKRKSKDEAPSPLNGDASDGASVGGIGGAGGVNMVNASGIPISASGGGLATQPQSLLNPVTGLNVQISTKKCKTASPCAISPVLLECPEQDCSKKYKHANGLRYHQSHAHGAGGGASSMDEDSMQAPEDPATPPSPGVASGTGSGASVASSAVPATAPSAGQGTVAVSPNTPLANSSNPVTNGNVAPSAPATGSVTIAAPNTTPSVVETQAPLTGPPPVTPPAPTPICAVATPGAEQSVSSVLPLGNLPLTAGPNSATQQQQPPTQQQQPQLLVPGGSAASLQQQQQQQQPVAGGSITAGISGQALSQHQQQLMGGLPAMLSDQQQQALLQQGALKAGVLRFGPPDGNPLQQQPGQASVNPQTQQSPPRPPSHVQDQQTPSAYAQQAGLKTSPGFGSVGVGAASSKQKKNRKSPGPSDFEGRVSREDVQSPAYSDISDDSTPVAEQEMLDKSVGQAVTAKHIELMGKKPTEVGVGVPPPPAPNMYVPGMYQFYPAQQQSAPPPQQQQQQPQYMVQTEPGKPPGLPPALTQAQQQQQLQPGAPPPTSQPPSHLLGPPGQQSVAAHLADYSGKNKDPPLDLMTKPQPQPGQPPSQQQQSGQLSGQENNGKDVGPPTSQPGSQPPPVNLSAVAGPPPGSLPPGLGGLSALGAAGLGGPGPGKGMPHFYPFNFIPPAYPYNVDPNFGSVSIVASEEAAKLSGHPGLPPSSQAQQLSGISIKEERLKESPSPHDQPKHMPSQQQMIASKLIKQEPMTKQEIKQEPNSNPGQQHPPPQQQPAPQPQQQQPPPPQPQQPHALHPKDLQALGAYPAIYQRHSINLAVQQAREEELRRYYMFTGRQNSAAAAAAAAAQNAASGGLPPHPGMMHKDEPGMGSAAQQQQQQQQQQMQIAQQQQQAIQQHHQHLQQQHQAQQQQQQQQHQQQQQQQQQQQQQQQQQQQQQKLKQSQAASAGANNKATNLTKDSPKQKGGDDDQPLKVKQEGQKPTMETQGPPPPPTSQYFLHPSYISPTPFGFDPNHPMYRNVLMSAAGPYNTAPYHLPIPRPYHAPEDLSRNTGTKALDALHHAASQYYTTHKIHELSERALKSPTSGSGPVKVSVSSPSIGPPQPGGPTSSGPGSGPVSGVLGPGSGSNQQPGSAPGSAGGVPLNLQPPPGGMGPSPGSKPDLSGPKGHGGVTPGSSLDGHKQSMPGGPPPNGPSGNGAVGGVGGAAGNGAAGGGGAGAADSRSPPPQRHVHTHHHTHVGLGYPMYPAPYGAAVLASQQAAAVAVINPFPPGPSK

Target ORF information:

RefSeq Version	NM_001169720.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169720.2

ORF Insert Sequence:

ATGGAGTCAA TGAGACGTAA CGAGGCGAAC ACTAGCCACC ACCCGAATAA GGTGGCCCTG 
AATAGCAGCA ACGTAGCAAC GGAAAGCAAT ATTAAGAATA ATAAATTGTT AGCTAATTTA 
AGTGCGGCAG GAGCAGCTAC AGCTACAACG GCAACAACAG GAACAGCAAC AACAACGACG 
GCGACAACAG CGAATCAAGC GCTGAATTTC AATAACAAAA CGAAAGCAAC AGCAAACGCG 
ACTGCAGCAT CGGCGAATAA TCGGCACAAT AACAACAACA ATTCCAGTGC GATCAAGAAG 
CACACAAATA CGAAACAACT AGCTGGCAAG AGCCCCGCGT GCAACTCCTC CTCCTCGTCG 
CTCTCCTCGT CGAGCAGCTC CAACTCGAGC GAGTCGAAGG ACACGAACTT TGAGTACGAG 
GACGAGTGGA ATATTGGCGG CATACCAGAG CTGCTGGACG ACTTGGACGC GGATATTGAG 
AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC CAGGCTACCG CGCTAAATGC CAAGCAGGCA 
AACAGCTCCT CCACATCCTC CTCATCATCC TCCAAGGGCG GCGCTTCATC CTCGTCATCC 
TCAGCAGCAG CGACGGGATC ATCATCGTCG CATAAATCGC ACAAAACCAC ATTGCACAGC 
AATTTGTCGG CCACATCGCC AACCACAATT AAGTTCACAC GCCAACCGGT GGCCATTGGC 
GGAGCTAACT CCTCCTCATC TTCATCAGCA GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC 
GTGGTGGCCA AGGGATCATC TTCTTCCTCG TCCTCCACAT CTTCCTCATC TTCGGGGAAG 
CATCATCACC ATCACCACCA TCACTCAAAC TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC 
TACAAGTCCG CTTTGGTGGC TCAACTGAAC AGTCCGAGTC CACTGAACAG TAACTCCAAG 
TCCCTGAGTG GATCGGGGAG TGGAAGCGGG AACACAAACG GAGCAGCGGG AGCTGGAGCC 
GGCAGCACAT TGTCATCCTC GACATTTGCC GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT 
TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG GCTGGCAGTG GACAACAGGG CTCCAAATTC 
TCCGCTGGCG GCATGTCCTC GCAAACGGGC AGCGGCAGCG GCGGCAACAA CACTAGCAAT 
AGCAACAACA GCAGCAGCGG AAGCGGAGGC AGCGGCTCAG GTAGCAGCAC AGGGAACACC 
GCCAGCGGTA GCGGGAACAA CAACAGCACA AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC 
GGCAACAACG GAAACGGTAG CGGTAGCAGC TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA 
AAGATGTCCA TAGACCACCA AGCGACGCTC GACAAAGGAC TCAAAATGAA GATCAAGCGC 
ACCAAGCCGG GCACCAAAAG CTCGGAGGCC AAACACGAGA TTGTGAAGGC CACCGATCAG 
CAACAGAACG GAGCCCTGGG CGCCGGATCC AATAACTCGG CCAACGAGGA TGGGAGTTCC 
GGTTCCAGTT CCACCAACGC GTCTTCCCTG GGGAGCACCA ATTCATCGAG CAGCGCAAGC 
AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT TCGAGCAGCA GCAAGAAGCA CCTAAACAAT 
GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC GGAGGAGGGA GCCAAAACAA TGCCAGTGGC 
CATGCCAGCG GAGGGGGATC CTCCGGTAGC AGTCAGTCTA CGCCGCAGGG CACTAAGCGC 
GGTAGTTCGG GTCATCGGCG TGAGAAGACC AAGGACAAGA ACGCACATTC CAATCGCATG 
TCCGTGGACA AGTCAGCAGC TGCTGCCTCG GCGGCCGGCG AGAAGGATAC ACCTGAGAAG 
TGTTCCGGTA CGGGAGCTGG TGGATCTCCC TGCTCCTGCA ACGGAGATGT GGGAGCTCCT 
TGCTCACATC ATGCCTGCAT TCGCCGTGCC GCACACATGT CCAACTCCGC AGGCAATGCG 
AATTCGAGTG CCGGCACTGG GCAATCCGGT GGTTCATCCT CCATGTCAGC AGTGCCACCG 
GGTGTGTTCA CACCTTCAGC GGGCTCTCCT TCCACCGGTT CACCATCGAC CGTGGTGCCT 
GCCGCTGCCT CACTTCTGGC AGCGACCGGA GCAGCCTCCT CCTCCGCCTC TCAAATGGCC 
AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG GGAGGAGGTG CCAATGCACC AGGACCGCCG 
GGTAAGGAGT CTGCCGGCAG CATCAAAATC TCCTCGCACA TTGCCGCCCA ACTGGCAGCA 
GCGGCCGCTT CCAATAGTTA CAGCGGGAGT GGAGCCAACA CGAATCAAGG CCAGAACAGC 
AATGCTGGCG GCAACGGCGG TTCGGAGAGC AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA 
ATGGCACCCG GCATGATCTC AGCCACCATG CACCATACGA TCTCGGTGCC GGCGGGCACA 
GGAACAGGCG ACGACGATAC CAAATCTCCG CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC 
GCTAGTGGAG CAGGAGGCGG CAAGGAGATG GTGGACATCT GCATTGGCAC CTCGGTGGGC 
ACCATCACCG AACCGGACTG CCTGGGTCCC TGCGAACCTG GCACTTCCGT AACCCTCGAA 
GGGATTGTGT GGCACGAGAC CGAAGGCGGC GTTCTCGTGG TCAATGTCAC GTGGCGGGGC 
AAGACCTACG TGGGCACTCT GCTAGACTGC ACCCGACACG ATTGGGCTCC TCCAAGATTC 
TGTGATTCAC CAACTGAAGA ATTAGATTCT CGAACTCCAA AGGGACGCGG AAAGCGCGGA 
CGCAGTGCAG GTCTCACTCC TGACCTGAGC AACTTCACCG AGACCAGAAG TTCGATTTAC 
TTCTCACACG CCCAGGTGCA CTCCAAGCTG CGTAATGGTG CCACAAAGGG ACGCGGTGCG 
ACACGCAGTG CATCCGGTAA TGCGGCAGCG AACAGCAACA GCAGTTCATC GGGCAACGGA 
GGCGGGGCCA CGCCCAGCAC ATCGCCGACG GCATTTTTGC CGCCACGGCC AGAGAAGCGC 
AAGTCGAAGG ATGAGGCGCC TTCACCGCTT AACGGCGACG CCTCGGATGG AGCGTCTGTT 
GGCGGTATTG GTGGAGCCGG TGGAGTGAAC ATGGTGAACG CCAGTGGCAT CCCCATATCG 
GCCAGCGGCG GTGGCCTGGC CACGCAGCCG CAGAGCCTGC TCAATCCGGT CACTGGTCTC 
AATGTGCAGA TCAGCACCAA GAAATGTAAG ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT 
CTGCTTGAGT GTCCCGAGCA GGACTGCAGC AAGAAGTACA AGCACGCAAA TGGGCTGCGT 
TATCACCAGT CGCATGCCCA TGGAGCGGGC GGTGGCGCAA GCTCCATGGA TGAGGACTCC 
ATGCAGGCGC CCGAGGATCC GGCCACGCCG CCCTCGCCAG GAGTCGCAAG TGGAACGGGA 
TCAGGAGCAT CTGTAGCATC ATCGGCAGTA CCAGCGACAG CACCATCAGC GGGTCAAGGA 
ACTGTCGCAG TGTCCCCAAA CACACCCCTC GCAAATTCAA GCAACCCCGT CACCAATGGC 
AATGTTGCAC CATCGGCACC AGCTACTGGA TCGGTAACTA TCGCCGCTCC CAACACTACT 
CCCTCTGTGG TAGAGACGCA AGCGCCGCTT ACTGGTCCTC CACCAGTCAC GCCACCAGCT 
CCAACTCCCA TCTGCGCAGT TGCAACACCC GGAGCGGAGC AATCTGTATC ATCAGTATTG 
CCCCTGGGAA ATCTTCCACT GACGGCAGGT CCAAATAGCG CGACACAACA GCAACAGCCT 
CCTACACAGC AGCAACAACC ACAGTTGCTC GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG 
CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC GGAGGCAGCA TCACTGCTGG AATCTCCGGA 
CAGGCTCTGT CACAGCATCA ACAGCAACTA ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC 
CAGCAGCAGC AGGCATTGTT GCAGCAGGGA GCACTTAAAG CGGGTGTACT GCGTTTTGGC 
CCGCCAGATG GAAATCCCCT GCAGCAGCAG CCTGGTCAAG CATCGGTTAA TCCACAGACA 
CAACAGTCTC CTCCAAGGCC TCCCAGTCAT GTCCAGGATC AACAGACGCC ATCGGCCTAC 
GCCCAGCAGG CAGGTTTAAA GACATCGCCT GGATTTGGCT CCGTTGGCGT GGGTGCCGCT 
AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA CCTGGACCCA GTGACTTTGA GGGCCGCGTA 
TCGCGCGAGG ATGTACAGAG TCCGGCATAC AGTGATATCT CCGATGACTC CACGCCAGTT 
GCCGAACAGG AAATGCTGGA CAAGTCAGTC GGCCAGGCGG TGACGGCAAA GCACATCGAA 
TTGATGGGCA AGAAGCCGAC GGAAGTGGGC GTTGGTGTTC CGCCACCACC AGCTCCCAAT 
ATGTATGTGC CGGGAATGTA TCAGTTCTAT CCGGCGCAGC AGCAGTCAGC ACCTCCACCA 
CAGCAACAGC AACAACAGCC GCAATACATG GTGCAGACAG AGCCAGGCAA ACCACCAGGA 
TTGCCACCTG CTTTGACACA AGCTCAGCAG CAGCAACAAT TGCAGCCGGG TGCTCCTCCG 
CCTACCTCAC AGCCCCCGAG TCATTTGCTA GGTCCACCCG GTCAGCAGTC GGTGGCTGCC 
CACCTGGCAG ACTACAGTGG CAAGAACAAG GATCCACCAT TGGATCTGAT GACCAAACCG 
CAGCCACAGC CGGGTCAGCC GCCTTCACAG CAGCAGCAGT CGGGCCAACT GTCGGGGCAG 
GAAAACAATG GCAAGGATGT TGGACCGCCC ACCTCTCAGC CAGGATCTCA GCCGCCGCCG 
GTTAACCTAA GTGCAGTGGC TGGGCCGCCG CCAGGAAGCC TGCCACCCGG TTTGGGTGGT 
CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT GGTCCTGGAC CGGGCAAGGG CATGCCCCAC 
TTCTATCCCT TCAATTTTAT TCCGCCTGCG TATCCATACA ATGTGGACCC CAACTTTGGG 
TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC GCCAAGCTGA GTGGGCATCC GGGTCTGCCA 
CCCAGCTCAC AAGCGCAGCA GTTATCCGGC ATAAGTATCA AGGAGGAGCG TCTGAAGGAA 
AGCCCCAGTC CGCACGACCA GCCGAAGCAC ATGCCATCTC AGCAACAGAT GATAGCCAGC 
AAGCTAATCA AGCAGGAGCC GATGACCAAG CAGGAGATCA AGCAGGAGCC CAACTCAAAT 
CCGGGCCAGC AGCATCCGCC TCCGCAACAG CAGCCAGCGC CTCAGCCGCA GCAACAACAG 
CCGCCACCCC CGCAGCCACA ACAGCCGCAT GCCCTGCATC CCAAGGATTT ACAGGCACTG 
GGCGCCTATC CCGCCATCTA CCAGCGCCAT TCCATAAACC TGGCCGTGCA GCAGGCTCGC 
GAGGAGGAGC TGAGACGGTA TTACATGTTC ACTGGACGAC AGAATTCGGC AGCCGCAGCA 
GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT GGTGGTCTTC CACCGCATCC CGGCATGATG 
CACAAGGATG AGCCTGGCAT GGGATCGGCG GCGCAACAAC AGCAGCAGCA ACAACAGCAG 
CAGATGCAAA TTGCACAGCA GCAGCAGCAG GCCATTCAGC AGCATCATCA GCACCTGCAG 
CAGCAGCACC AGGCCCAGCA ACAGCAGCAG CAGCAACAAC ACCAGCAGCA GCAGCAACAA 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAACAAC AGCAAAAGTT AAAGCAATCG 
CAGGCGGCTA GTGCGGGGGC AAATAATAAG GCCACCAACC TGACGAAGGA TTCTCCCAAA 
CAAAAGGGCG GCGATGACGA TCAACCGCTG AAGGTGAAGC AGGAGGGCCA AAAGCCGACC 
ATGGAGACGC AGGGTCCGCC ACCACCACCC ACGTCGCAGT ACTTCCTTCA CCCCTCATAC 
ATTTCCCCGA CGCCCTTTGG GTTTGATCCC AATCATCCGA TGTATCGCAA CGTGTTGATG 
TCAGCCGCCG GACCGTATAA TACGGCACCG TACCACCTGC CCATCCCTCG CCCGTACCAT 
GCCCCAGAAG ATCTCTCGCG TAACACCGGC ACCAAGGCGT TGGATGCACT CCATCACGCG 
GCCAGTCAGT ACTACACCAC CCACAAGATC CATGAGCTCA GCGAACGGGC CCTCAAGTCG 
CCCACCAGCG GCAGCGGCCC CGTCAAGGTG AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT 
CAACCAGGCG GACCCACGAG CAGCGGCCCC GGATCCGGAC CAGTTTCCGG TGTCCTCGGC 
CCCGGCAGCG GTTCCAACCA GCAGCCCGGC TCGGCACCTG GGTCTGCGGG CGGTGTGCCG 
CTGAACCTAC AGCCACCACC CGGAGGAATG GGCCCGTCAC CCGGCAGCAA GCCGGATCTG 
TCCGGCCCGA AAGGACATGG CGGAGTGACG CCCGGCTCGT CATTGGACGG CCACAAGCAG 
TCGATGCCCG GAGGTCCGCC ACCGAACGGG CCATCGGGCA ATGGAGCCGT TGGCGGTGTG 
GGAGGCGCTG CCGGCAATGG CGCAGCGGGA GGCGGCGGTG CAGGTGCCGC CGACTCACGC 
AGCCCACCAC CGCAGCGCCA TGTGCACACC CACCACCACA CGCACGTCGG CCTCGGCTAT 
CCCATGTACC CGGCGCCGTA TGGAGCGGCT GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA 
GCTGTGATAA ACCCGTTTCC GCCGGGTCCG TCGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sbb ( NM_001274145.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261074.1 Drosophila melanogaster scribbler (sbb), transcript variant J, mRNA.

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CloneID	OFa12208
Clone ID Related Accession (Same CDS sequence)	NM_001274145.1
Accession Version	NM_001274145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	scribbler, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTCAA TGAGACGTAA CGAGGCGAAC ACTAGCCACC ACCCGAATAA GGTGGCCCTG 
AATAGCAGCA ACGTAGCAAC GGAAAGCAAT ATTAAGAATA ATAAATTGTT AGCTAATTTA 
AGTGCGGCAG GAGCAGCTAC AGCTACAACG GCAACAACAG GAACAGCAAC AACAACGACG 
GCGACAACAG CGAATCAAGC GCTGAATTTC AATAACAAAA CGAAAGCAAC AGCAAACGCG 
ACTGCAGCAT CGGCGAATAA TCGGCACAAT AACAACAACA ATTCCAGTGC GATCAAGAAG 
CACACAAATA CGAAACAACT AGCTGGCAAG AGCCCCGCGT GCAACTCCTC CTCCTCGTCG 
CTCTCCTCGT CGAGCAGCTC CAACTCGAGC GAGTCGAAGG ACACGAACTT TGAGTACGAG 
GACGAGTGGA ATATTGGCGG CATACCAGAG CTGCTGGACG ACTTGGACGC GGATATTGAG 
AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC CAGGCTACCG CGCTAAATGC CAAGCAGGCA 
AACAGCTCCT CCACATCCTC CTCATCATCC TCCAAGGGCG GCGCTTCATC CTCGTCATCC 
TCAGCAGCAG CGACGGGATC ATCATCGTCG CATAAATCGC ACAAAACCAC ATTGCACAGC 
AATTTGTCGG CCACATCGCC AACCACAATT AAGTTCACAC GCCAACCGGT GGCCATTGGC 
GGAGCTAACT CCTCCTCATC TTCATCAGCA GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC 
GTGGTGGCCA AGGGATCATC TTCTTCCTCG TCCTCCACAT CTTCCTCATC TTCGGGGAAG 
CATCATCACC ATCACCACCA TCACTCAAAC TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC 
TACAAGTCCG CTTTGGTGGC TCAACTGAAC AGTCCGAGTC CACTGAACAG TAACTCCAAG 
TCCCTGAGTG GATCGGGGAG TGGAAGCGGG AACACAAACG GAGCAGCGGG AGCTGGAGCC 
GGCAGCACAT TGTCATCCTC GACATTTGCC GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT 
TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG GCTGGCAGTG GACAACAGGG CTCCAAATTC 
TCCGCTGGCG GCATGTCCTC GCAAACGGGC AGCGGCAGCG GCGGCAACAA CACTAGCAAT 
AGCAACAACA GCAGCAGCGG AAGCGGAGGC AGCGGCTCAG GTAGCAGCAC AGGGAACACC 
GCCAGCGGTA GCGGGAACAA CAACAGCACA AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC 
GGCAACAACG GAAACGGTAG CGGTAGCAGC TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA 
AAGATGTCCA TAGACCACCA AGCGACGCTC GACAAAGGAC TCAAAATGAA GATCAAGCGC 
ACCAAGCCGG GCACCAAAAG CTCGGAGGCC AAACACGAGA TTGTGAAGGC CACCGATCAG 
CAACAGAACG GAGCCCTGGG CGCCGGATCC AATAACTCGG CCAACGAGGA TGGGAGTTCC 
GGTTCCAGTT CCACCAACGC GTCTTCCCTG GGGAGCACCA ATTCATCGAG CAGCGCAAGC 
AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT TCGAGCAGCA GCAAGAAGCA CCTAAACAAT 
GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC GGAGGAGGGA GCCAAAACAA TGCCAGTGGC 
CATGCCAGCG GAGGGGGATC CTCCGGTAGC AGTCAGTCTA CGCCGCAGGG CACTAAGCGC 
GGTAGTTCGG GTCATCGGCG TGAGAAGACC AAGGACAAGA ACGCACATTC CAATCGCATG 
TCCGTGGACA AGTCAGCAGC TGCTGCCTCG GCGGCCGGCG AGAAGGATAC ACCTGAGAAG 
TGTTCCGGTA CGGGAGCTGG TGGATCTCCC TGCTCCTGCA ACGGAGATGT GGGAGCTCCT 
TGCTCACATC ATGCCTGCAT TCGCCGTGCC GCACACATGT CCAACTCCGC AGGCAATGCG 
AATTCGAGTG CCGGCACTGG GCAATCCGGT GGTTCATCCT CCATGTCAGC AGTGCCACCG 
GGTGTGTTCA CACCTTCAGC GGGCTCTCCT TCCACCGGTT CACCATCGAC CGTGGTGCCT 
GCCGCTGCCT CACTTCTGGC AGCGACCGGA GCAGCCTCCT CCTCCGCCTC TCAAATGGCC 
AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG GGAGGAGGTG CCAATGCACC AGGACCGCCG 
GGTAAGGAGT CTGCCGGCAG CATCAAAATC TCCTCGCACA TTGCCGCCCA ACTGGCAGCA 
GCGGCCGCTT CCAATAGTTA CAGCGGGAGT GGAGCCAACA CGAATCAAGG CCAGAACAGC 
AATGCTGGCG GCAACGGCGG TTCGGAGAGC AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA 
ATGGCACCCG GCATGATCTC AGCCACCATG CACCATACGA TCTCGGTGCC GGCGGGCACA 
GGAACAGGCG ACGACGATAC CAAATCTCCG CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC 
GCTAGTGGAG CAGGAGGCGG CAAGGAGATG GTGGACATCT GCATTGGCAC CTCGGTGGGC 
ACCATCACCG AACCGGACTG CCTGGGTCCC TGCGAACCTG GCACTTCCGT AACCCTCGAA 
GGGATTGTGT GGCACGAGAC CGAAGGCGGC GTTCTCGTGG TCAATGTCAC GTGGCGGGGC 
AAGACCTACG TGGGCACTCT GCTAGACTGC ACCCGACACG ATTGGGCTCC TCCAAGATTC 
TGTGATTCAC CAACTGAAGA ATTAGATTCT CGAACTCCAA AGGGACGCGG AAAGCGCGGA 
CGCAGTGCAG GTCTCACTCC TGACCTGAGC AACTTCACCG AGACCAGAAG TTCGATTTAC 
TTCTCACACG CCCAGGTGCA CTCCAAGCTG CGTAATGGTG CCACAAAGGG ACGCGGTGCG 
ACACGCAGTG CATCCGGTAA TGCGGCAGCG AACAGCAACA GCAGTTCATC GGGCAACGGA 
GGCGGGGCCA CGCCCAGCAC ATCGCCGACG GCATTTTTGC CGCCACGGCC AGAGAAGCGC 
AAGTCGAAGG ATGAGGCGCC TTCACCGCTT AACGGCGACG CCTCGGATGG AGCGTCTGTT 
GGCGGTATTG GTGGAGCCGG TGGAGTGAAC ATGGTGAACG CCAGTGGCAT CCCCATATCG 
GCCAGCGGCG GTGGCCTGGC CACGCAGCCG CAGAGCCTGC TCAATCCGGT CACTGGTCTC 
AATGTGCAGA TCAGCACCAA GAAATGTAAG ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT 
CTGCTTGAGT GTCCCGAGCA GGACTGCAGC AAGAAGTACA AGCACGCAAA TGGGCTGCGT 
TATCACCAGT CGCATGCCCA TGGAGCGGGC GGTGGCGCAA GCTCCATGGA TGAGGACTCC 
ATGCAGGCGC CCGAGGATCC GGCCACGCCG CCCTCGCCAG GAGTCGCAAG TGGAACGGGA 
TCAGGAGCAT CTGTAGCATC ATCGGCAGTA CCAGCGACAG CACCATCAGC GGGTCAAGGA 
ACTGTCGCAG TGTCCCCAAA CACACCCCTC GCAAATTCAA GCAACCCCGT CACCAATGGC 
AATGTTGCAC CATCGGCACC AGCTACTGGA TCGGTAACTA TCGCCGCTCC CAACACTACT 
CCCTCTGTGG TAGAGACGCA AGCGCCGCTT ACTGGTCCTC CACCAGTCAC GCCACCAGCT 
CCAACTCCCA TCTGCGCAGT TGCAACACCC GGAGCGGAGC AATCTGTATC ATCAGTATTG 
CCCCTGGGAA ATCTTCCACT GACGGCAGGT CCAAATAGCG CGACACAACA GCAACAGCCT 
CCTACACAGC AGCAACAACC ACAGTTGCTC GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG 
CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC GGAGGCAGCA TCACTGCTGG AATCTCCGGA 
CAGGCTCTGT CACAGCATCA ACAGCAACTA ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC 
CAGCAGCAGC AGGCATTGTT GCAGCAGGGA GCACTTAAAG CGGGTGTACT GCGTTTTGGC 
CCGCCAGATG GAAATCCCCT GCAGCAGCAG CCTGGTCAAG CATCGGTTAA TCCACAGACA 
CAACAGTCTC CTCCAAGGCC TCCCAGTCAT GTCCAGGATC AACAGACGCC ATCGGCCTAC 
GCCCAGCAGG CAGGTTTAAA GACATCGCCT GGATTTGGCT CCGTTGGCGT GGGTGCCGCT 
AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA CCTGGACCCA GTGACTTTGA GGGCCGCGTA 
TCGCGCGAGG ATGTACAGAG TCCGGCATAC AGTGATATCT CCGATGACTC CACGCCAGTT 
GCCGAACAGG AAATGCTGGA CAAGTCAGTC GGCCAGGCGG TGACGGCAAA GCACATCGAA 
TTGATGGGCA AGAAGCCGAC GGAAGTGGGC GTTGGTGTTC CGCCACCACC AGCTCCCAAT 
ATGTATGTGC CGGGAATGTA TCAGTTCTAT CCGGCGCAGC AGCAGTCAGC ACCTCCACCA 
CAGCAACAGC AACAACAGCC GCAATACATG GTGCAGACAG AGCCAGGCAA ACCACCAGGA 
TTGCCACCTG CTTTGACACA AGCTCAGCAG CAGCAACAAT TGCAGCCGGG TGCTCCTCCG 
CCTACCTCAC AGCCCCCGAG TCATTTGCTA GGTCCACCCG GTCAGCAGTC GGTGGCTGCC 
CACCTGGCAG ACTACAGTGG CAAGAACAAG GATCCACCAT TGGATCTGAT GACCAAACCG 
CAGCCACAGC CGGGTCAGCC GCCTTCACAG CAGCAGCAGT CGGGCCAACT GTCGGGGCAG 
GAAAACAATG GCAAGGATGT TGGACCGCCC ACCTCTCAGC CAGGATCTCA GCCGCCGCCG 
GTTAACCTAA GTGCAGTGGC TGGGCCGCCG CCAGGAAGCC TGCCACCCGG TTTGGGTGGT 
CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT GGTCCTGGAC CGGGCAAGGG CATGCCCCAC 
TTCTATCCCT TCAATTTTAT TCCGCCTGCG TATCCATACA ATGTGGACCC CAACTTTGGG 
TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC GCCAAGCTGA GTGGGCATCC GGGTCTGCCA 
CCCAGCTCAC AAGCGCAGCA GTTATCCGGC ATAAGTATCA AGGAGGAGCG TCTGAAGGAA 
AGCCCCAGTC CGCACGACCA GCCGAAGCAC ATGCCATCTC AGCAACAGAT GATAGCCAGC 
AAGCTAATCA AGCAGGAGCC GATGACCAAG CAGGAGATCA AGCAGGAGCC CAACTCAAAT 
CCGGGCCAGC AGCATCCGCC TCCGCAACAG CAGCCAGCGC CTCAGCCGCA GCAACAACAG 
CCGCCACCCC CGCAGCCACA ACAGCCGCAT GCCCTGCATC CCAAGGATTT ACAGGCACTG 
GGCGCCTATC CCGCCATCTA CCAGCGCCAT TCCATAAACC TGGCCGTGCA GCAGGCTCGC 
GAGGAGGAGC TGAGACGGTA TTACATGTTC ACTGGACGAC AGAATTCGGC AGCCGCAGCA 
GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT GGTGGTCTTC CACCGCATCC CGGCATGATG 
CACAAGGATG AGCCTGGCAT GGGATCGGCG GCGCAACAAC AGCAGCAGCA ACAACAGCAG 
CAGATGCAAA TTGCACAGCA GCAGCAGCAG GCCATTCAGC AGCATCATCA GCACCTGCAG 
CAGCAGCACC AGGCCCAGCA ACAGCAGCAG CAGCAACAAC ACCAGCAGCA GCAGCAACAA 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAACAAC AGCAAAAGTT AAAGCAATCG 
CAGGCGGCTA GTGCGGGGGC AAATAATAAG GCCACCAACC TGACGAAGGA TTCTCCCAAA 
CAAAAGGGCG GCGATGACGA TCAACCGCTG AAGGTGAAGC AGGAGGGCCA AAAGCCGACC 
ATGGAGACGC AGGGTCCGCC ACCACCACCC ACGTCGCAGT ACTTCCTTCA CCCCTCATAC 
ATTTCCCCGA CGCCCTTTGG GTTTGATCCC AATCATCCGA TGTATCGCAA CGTGTTGATG 
TCAGCCGCCG GACCGTATAA TACGGCACCG TACCACCTGC CCATCCCTCG CCCGTACCAT 
GCCCCAGAAG ATCTCTCGCG TAACACCGGC ACCAAGGCGT TGGATGCACT CCATCACGCG 
GCCAGTCAGT ACTACACCAC CCACAAGATC CATGAGCTCA GCGAACGGGC CCTCAAGTCG 
CCCACCAGCG GCAGCGGCCC CGTCAAGGTG AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT 
CAACCAGGCG GACCCACGAG CAGCGGCCCC GGATCCGGAC CAGTTTCCGG TGTCCTCGGC 
CCCGGCAGCG GTTCCAACCA GCAGCCCGGC TCGGCACCTG GGTCTGCGGG CGGTGTGCCG 
CTGAACCTAC AGCCACCACC CGGAGGAATG GGCCCGTCAC CCGGCAGCAA GCCGGATCTG 
TCCGGCCCGA AAGGACATGG CGGAGTGACG CCCGGCTCGT CATTGGACGG CCACAAGCAG 
TCGATGCCCG GAGGTCCGCC ACCGAACGGG CCATCGGGCA ATGGAGCCGT TGGCGGTGTG 
GGAGGCGCTG CCGGCAATGG CGCAGCGGGA GGCGGCGGTG CAGGTGCCGC CGACTCACGC 
AGCCCACCAC CGCAGCGCCA TGTGCACACC CACCACCACA CGCACGTCGG CCTCGGCTAT 
CCCATGTACC CGGCGCCGTA TGGAGCGGCT GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA 
GCTGTGATAA ACCCGTTTCC GCCGGGTCCG TCGAAATGAG ATCCACTGAA CGGTAACAAC 
GAGAAGAAGG CGTATAACGT AAAGAACAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261074.1
CDS	2063..9055
Translation	MESMRRNEANTSHHPNKVALNSSNVATESNIKNNKLLANLSAAGAATATTATTGTATTTTATTANQALNFNNKTKATANATAASANNRHNNNNNSSAIKKHTNTKQLAGKSPACNSSSSSLSSSSSSNSSESKDTNFEYEDEWNIGGIPELLDDLDADIEKSAHSSGGGNQATALNAKQANSSSTSSSSSSKGGASSSSSSAAATGSSSSHKSHKTTLHSNLSATSPTTIKFTRQPVAIGGANSSSSSSAAAAPSGGSANVVAKGSSSSSSSTSSSSSGKHHHHHHHHSNSSSSGSSSKGYKSALVAQLNSPSPLNSNSKSLSGSGSGSGNTNGAAGAGAGSTLSSSTFAGFSKGGSLVSSSAGAAAALAAGSGQQGSKFSAGGMSSQTGSGSGGNNTSNSNNSSSGSGGSGSGSSTGNTASGSGNNNSTSAGGPPSSQGGNNGNGSGSSSSSSSGKSSAKMSIDHQATLDKGLKMKIKRTKPGTKSSEAKHEIVKATDQQQNGALGAGSNNSANEDGSSGSSSTNASSLGSTNSSSSASSGSSSSSGSSSSSSKKHLNNASSGSGSSSSGGGSQNNASGHASGGGSSGSSQSTPQGTKRGSSGHRREKTKDKNAHSNRMSVDKSAAAASAAGEKDTPEKCSGTGAGGSPCSCNGDVGAPCSHHACIRRAAHMSNSAGNANSSAGTGQSGGSSSMSAVPPGVFTPSAGSPSTGSPSTVVPAAASLLAATGAASSSASQMASSSAGGVGGSGGGANAPGPPGKESAGSIKISSHIAAQLAAAAASNSYSGSGANTNQGQNSNAGGNGGSESKAAAAAQAKLMAPGMISATMHHTISVPAGTGTGDDDTKSPPAKRVKHEAGASGAGGGKEMVDICIGTSVGTITEPDCLGPCEPGTSVTLEGIVWHETEGGVLVVNVTWRGKTYVGTLLDCTRHDWAPPRFCDSPTEELDSRTPKGRGKRGRSAGLTPDLSNFTETRSSIYFSHAQVHSKLRNGATKGRGATRSASGNAAANSNSSSSGNGGGATPSTSPTAFLPPRPEKRKSKDEAPSPLNGDASDGASVGGIGGAGGVNMVNASGIPISASGGGLATQPQSLLNPVTGLNVQISTKKCKTASPCAISPVLLECPEQDCSKKYKHANGLRYHQSHAHGAGGGASSMDEDSMQAPEDPATPPSPGVASGTGSGASVASSAVPATAPSAGQGTVAVSPNTPLANSSNPVTNGNVAPSAPATGSVTIAAPNTTPSVVETQAPLTGPPPVTPPAPTPICAVATPGAEQSVSSVLPLGNLPLTAGPNSATQQQQPPTQQQQPQLLVPGGSAASLQQQQQQQQPVAGGSITAGISGQALSQHQQQLMGGLPAMLSDQQQQALLQQGALKAGVLRFGPPDGNPLQQQPGQASVNPQTQQSPPRPPSHVQDQQTPSAYAQQAGLKTSPGFGSVGVGAASSKQKKNRKSPGPSDFEGRVSREDVQSPAYSDISDDSTPVAEQEMLDKSVGQAVTAKHIELMGKKPTEVGVGVPPPPAPNMYVPGMYQFYPAQQQSAPPPQQQQQQPQYMVQTEPGKPPGLPPALTQAQQQQQLQPGAPPPTSQPPSHLLGPPGQQSVAAHLADYSGKNKDPPLDLMTKPQPQPGQPPSQQQQSGQLSGQENNGKDVGPPTSQPGSQPPPVNLSAVAGPPPGSLPPGLGGLSALGAAGLGGPGPGKGMPHFYPFNFIPPAYPYNVDPNFGSVSIVASEEAAKLSGHPGLPPSSQAQQLSGISIKEERLKESPSPHDQPKHMPSQQQMIASKLIKQEPMTKQEIKQEPNSNPGQQHPPPQQQPAPQPQQQQPPPPQPQQPHALHPKDLQALGAYPAIYQRHSINLAVQQAREEELRRYYMFTGRQNSAAAAAAAAAQNAASGGLPPHPGMMHKDEPGMGSAAQQQQQQQQQQMQIAQQQQQAIQQHHQHLQQQHQAQQQQQQQQHQQQQQQQQQQQQQQQQQQQQQKLKQSQAASAGANNKATNLTKDSPKQKGGDDDQPLKVKQEGQKPTMETQGPPPPPTSQYFLHPSYISPTPFGFDPNHPMYRNVLMSAAGPYNTAPYHLPIPRPYHAPEDLSRNTGTKALDALHHAASQYYTTHKIHELSERALKSPTSGSGPVKVSVSSPSIGPPQPGGPTSSGPGSGPVSGVLGPGSGSNQQPGSAPGSAGGVPLNLQPPPGGMGPSPGSKPDLSGPKGHGGVTPGSSLDGHKQSMPGGPPPNGPSGNGAVGGVGGAAGNGAAGGGGAGAADSRSPPPQRHVHTHHHTHVGLGYPMYPAPYGAAVLASQQAAAVAVINPFPPGPSK*DPLNGNNEKKAYNVKNN

Target ORF information:

RefSeq Version	NM_001274145.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster scribbler (sbb), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274145.1

ORF Insert Sequence:

ATGGAGTCAA TGAGACGTAA CGAGGCGAAC ACTAGCCACC ACCCGAATAA GGTGGCCCTG 
AATAGCAGCA ACGTAGCAAC GGAAAGCAAT ATTAAGAATA ATAAATTGTT AGCTAATTTA 
AGTGCGGCAG GAGCAGCTAC AGCTACAACG GCAACAACAG GAACAGCAAC AACAACGACG 
GCGACAACAG CGAATCAAGC GCTGAATTTC AATAACAAAA CGAAAGCAAC AGCAAACGCG 
ACTGCAGCAT CGGCGAATAA TCGGCACAAT AACAACAACA ATTCCAGTGC GATCAAGAAG 
CACACAAATA CGAAACAACT AGCTGGCAAG AGCCCCGCGT GCAACTCCTC CTCCTCGTCG 
CTCTCCTCGT CGAGCAGCTC CAACTCGAGC GAGTCGAAGG ACACGAACTT TGAGTACGAG 
GACGAGTGGA ATATTGGCGG CATACCAGAG CTGCTGGACG ACTTGGACGC GGATATTGAG 
AAGTCCGCGC ATTCTTCGGG TGGTGGCAAC CAGGCTACCG CGCTAAATGC CAAGCAGGCA 
AACAGCTCCT CCACATCCTC CTCATCATCC TCCAAGGGCG GCGCTTCATC CTCGTCATCC 
TCAGCAGCAG CGACGGGATC ATCATCGTCG CATAAATCGC ACAAAACCAC ATTGCACAGC 
AATTTGTCGG CCACATCGCC AACCACAATT AAGTTCACAC GCCAACCGGT GGCCATTGGC 
GGAGCTAACT CCTCCTCATC TTCATCAGCA GCTGCAGCGC CCAGTGGTGG CAGCGCGAAC 
GTGGTGGCCA AGGGATCATC TTCTTCCTCG TCCTCCACAT CTTCCTCATC TTCGGGGAAG 
CATCATCACC ATCACCACCA TCACTCAAAC TCAAGCAGCA GTGGAAGCAG TTCCAAGGGC 
TACAAGTCCG CTTTGGTGGC TCAACTGAAC AGTCCGAGTC CACTGAACAG TAACTCCAAG 
TCCCTGAGTG GATCGGGGAG TGGAAGCGGG AACACAAACG GAGCAGCGGG AGCTGGAGCC 
GGCAGCACAT TGTCATCCTC GACATTTGCC GGTTTCTCCA AAGGCGGCAG CTTAGTGTCT 
TCGTCGGCAG GAGCAGCAGC GGCTTTAGCG GCTGGCAGTG GACAACAGGG CTCCAAATTC 
TCCGCTGGCG GCATGTCCTC GCAAACGGGC AGCGGCAGCG GCGGCAACAA CACTAGCAAT 
AGCAACAACA GCAGCAGCGG AAGCGGAGGC AGCGGCTCAG GTAGCAGCAC AGGGAACACC 
GCCAGCGGTA GCGGGAACAA CAACAGCACA AGTGCCGGTG GGCCGCCGAG TTCGCAAGGC 
GGCAACAACG GAAACGGTAG CGGTAGCAGC TCCAGCTCCT CCAGTGGTAA ATCGAGCGCA 
AAGATGTCCA TAGACCACCA AGCGACGCTC GACAAAGGAC TCAAAATGAA GATCAAGCGC 
ACCAAGCCGG GCACCAAAAG CTCGGAGGCC AAACACGAGA TTGTGAAGGC CACCGATCAG 
CAACAGAACG GAGCCCTGGG CGCCGGATCC AATAACTCGG CCAACGAGGA TGGGAGTTCC 
GGTTCCAGTT CCACCAACGC GTCTTCCCTG GGGAGCACCA ATTCATCGAG CAGCGCAAGC 
AGCGGCAGCT CCTCCAGCAG CGGCAGTTCT TCGAGCAGCA GCAAGAAGCA CCTAAACAAT 
GCCAGTAGCG GTAGTGGCTC CTCCTCTTCC GGAGGAGGGA GCCAAAACAA TGCCAGTGGC 
CATGCCAGCG GAGGGGGATC CTCCGGTAGC AGTCAGTCTA CGCCGCAGGG CACTAAGCGC 
GGTAGTTCGG GTCATCGGCG TGAGAAGACC AAGGACAAGA ACGCACATTC CAATCGCATG 
TCCGTGGACA AGTCAGCAGC TGCTGCCTCG GCGGCCGGCG AGAAGGATAC ACCTGAGAAG 
TGTTCCGGTA CGGGAGCTGG TGGATCTCCC TGCTCCTGCA ACGGAGATGT GGGAGCTCCT 
TGCTCACATC ATGCCTGCAT TCGCCGTGCC GCACACATGT CCAACTCCGC AGGCAATGCG 
AATTCGAGTG CCGGCACTGG GCAATCCGGT GGTTCATCCT CCATGTCAGC AGTGCCACCG 
GGTGTGTTCA CACCTTCAGC GGGCTCTCCT TCCACCGGTT CACCATCGAC CGTGGTGCCT 
GCCGCTGCCT CACTTCTGGC AGCGACCGGA GCAGCCTCCT CCTCCGCCTC TCAAATGGCC 
AGCAGTAGTG CCGGCGGCGT TGGCGGTTCG GGAGGAGGTG CCAATGCACC AGGACCGCCG 
GGTAAGGAGT CTGCCGGCAG CATCAAAATC TCCTCGCACA TTGCCGCCCA ACTGGCAGCA 
GCGGCCGCTT CCAATAGTTA CAGCGGGAGT GGAGCCAACA CGAATCAAGG CCAGAACAGC 
AATGCTGGCG GCAACGGCGG TTCGGAGAGC AAAGCAGCAG CGGCTGCTCA GGCCAAGTTA 
ATGGCACCCG GCATGATCTC AGCCACCATG CACCATACGA TCTCGGTGCC GGCGGGCACA 
GGAACAGGCG ACGACGATAC CAAATCTCCG CCTGCCAAAA GGGTCAAGCA TGAGGCTGGC 
GCTAGTGGAG CAGGAGGCGG CAAGGAGATG GTGGACATCT GCATTGGCAC CTCGGTGGGC 
ACCATCACCG AACCGGACTG CCTGGGTCCC TGCGAACCTG GCACTTCCGT AACCCTCGAA 
GGGATTGTGT GGCACGAGAC CGAAGGCGGC GTTCTCGTGG TCAATGTCAC GTGGCGGGGC 
AAGACCTACG TGGGCACTCT GCTAGACTGC ACCCGACACG ATTGGGCTCC TCCAAGATTC 
TGTGATTCAC CAACTGAAGA ATTAGATTCT CGAACTCCAA AGGGACGCGG AAAGCGCGGA 
CGCAGTGCAG GTCTCACTCC TGACCTGAGC AACTTCACCG AGACCAGAAG TTCGATTTAC 
TTCTCACACG CCCAGGTGCA CTCCAAGCTG CGTAATGGTG CCACAAAGGG ACGCGGTGCG 
ACACGCAGTG CATCCGGTAA TGCGGCAGCG AACAGCAACA GCAGTTCATC GGGCAACGGA 
GGCGGGGCCA CGCCCAGCAC ATCGCCGACG GCATTTTTGC CGCCACGGCC AGAGAAGCGC 
AAGTCGAAGG ATGAGGCGCC TTCACCGCTT AACGGCGACG CCTCGGATGG AGCGTCTGTT 
GGCGGTATTG GTGGAGCCGG TGGAGTGAAC ATGGTGAACG CCAGTGGCAT CCCCATATCG 
GCCAGCGGCG GTGGCCTGGC CACGCAGCCG CAGAGCCTGC TCAATCCGGT CACTGGTCTC 
AATGTGCAGA TCAGCACCAA GAAATGTAAG ACTGCTTCGC CCTGCGCGAT CTCCCCAGTT 
CTGCTTGAGT GTCCCGAGCA GGACTGCAGC AAGAAGTACA AGCACGCAAA TGGGCTGCGT 
TATCACCAGT CGCATGCCCA TGGAGCGGGC GGTGGCGCAA GCTCCATGGA TGAGGACTCC 
ATGCAGGCGC CCGAGGATCC GGCCACGCCG CCCTCGCCAG GAGTCGCAAG TGGAACGGGA 
TCAGGAGCAT CTGTAGCATC ATCGGCAGTA CCAGCGACAG CACCATCAGC GGGTCAAGGA 
ACTGTCGCAG TGTCCCCAAA CACACCCCTC GCAAATTCAA GCAACCCCGT CACCAATGGC 
AATGTTGCAC CATCGGCACC AGCTACTGGA TCGGTAACTA TCGCCGCTCC CAACACTACT 
CCCTCTGTGG TAGAGACGCA AGCGCCGCTT ACTGGTCCTC CACCAGTCAC GCCACCAGCT 
CCAACTCCCA TCTGCGCAGT TGCAACACCC GGAGCGGAGC AATCTGTATC ATCAGTATTG 
CCCCTGGGAA ATCTTCCACT GACGGCAGGT CCAAATAGCG CGACACAACA GCAACAGCCT 
CCTACACAGC AGCAACAACC ACAGTTGCTC GTCCCAGGAG GCAGTGCGGC AAGCCTTCAG 
CAGCAGCAGC AGCAGCAGCA ACCAGTGGCC GGAGGCAGCA TCACTGCTGG AATCTCCGGA 
CAGGCTCTGT CACAGCATCA ACAGCAACTA ATGGGTGGAT TGCCTGCCAT GTTGTCAGAC 
CAGCAGCAGC AGGCATTGTT GCAGCAGGGA GCACTTAAAG CGGGTGTACT GCGTTTTGGC 
CCGCCAGATG GAAATCCCCT GCAGCAGCAG CCTGGTCAAG CATCGGTTAA TCCACAGACA 
CAACAGTCTC CTCCAAGGCC TCCCAGTCAT GTCCAGGATC AACAGACGCC ATCGGCCTAC 
GCCCAGCAGG CAGGTTTAAA GACATCGCCT GGATTTGGCT CCGTTGGCGT GGGTGCCGCT 
AGCAGCAAAC AAAAGAAGAA CCGCAAGTCA CCTGGACCCA GTGACTTTGA GGGCCGCGTA 
TCGCGCGAGG ATGTACAGAG TCCGGCATAC AGTGATATCT CCGATGACTC CACGCCAGTT 
GCCGAACAGG AAATGCTGGA CAAGTCAGTC GGCCAGGCGG TGACGGCAAA GCACATCGAA 
TTGATGGGCA AGAAGCCGAC GGAAGTGGGC GTTGGTGTTC CGCCACCACC AGCTCCCAAT 
ATGTATGTGC CGGGAATGTA TCAGTTCTAT CCGGCGCAGC AGCAGTCAGC ACCTCCACCA 
CAGCAACAGC AACAACAGCC GCAATACATG GTGCAGACAG AGCCAGGCAA ACCACCAGGA 
TTGCCACCTG CTTTGACACA AGCTCAGCAG CAGCAACAAT TGCAGCCGGG TGCTCCTCCG 
CCTACCTCAC AGCCCCCGAG TCATTTGCTA GGTCCACCCG GTCAGCAGTC GGTGGCTGCC 
CACCTGGCAG ACTACAGTGG CAAGAACAAG GATCCACCAT TGGATCTGAT GACCAAACCG 
CAGCCACAGC CGGGTCAGCC GCCTTCACAG CAGCAGCAGT CGGGCCAACT GTCGGGGCAG 
GAAAACAATG GCAAGGATGT TGGACCGCCC ACCTCTCAGC CAGGATCTCA GCCGCCGCCG 
GTTAACCTAA GTGCAGTGGC TGGGCCGCCG CCAGGAAGCC TGCCACCCGG TTTGGGTGGT 
CTCTCGGCTT TGGGAGCAGC CGGTCTAGGT GGTCCTGGAC CGGGCAAGGG CATGCCCCAC 
TTCTATCCCT TCAATTTTAT TCCGCCTGCG TATCCATACA ATGTGGACCC CAACTTTGGG 
TCAGTTTCTA TTGTTGCTTC AGAGGAGGCC GCCAAGCTGA GTGGGCATCC GGGTCTGCCA 
CCCAGCTCAC AAGCGCAGCA GTTATCCGGC ATAAGTATCA AGGAGGAGCG TCTGAAGGAA 
AGCCCCAGTC CGCACGACCA GCCGAAGCAC ATGCCATCTC AGCAACAGAT GATAGCCAGC 
AAGCTAATCA AGCAGGAGCC GATGACCAAG CAGGAGATCA AGCAGGAGCC CAACTCAAAT 
CCGGGCCAGC AGCATCCGCC TCCGCAACAG CAGCCAGCGC CTCAGCCGCA GCAACAACAG 
CCGCCACCCC CGCAGCCACA ACAGCCGCAT GCCCTGCATC CCAAGGATTT ACAGGCACTG 
GGCGCCTATC CCGCCATCTA CCAGCGCCAT TCCATAAACC TGGCCGTGCA GCAGGCTCGC 
GAGGAGGAGC TGAGACGGTA TTACATGTTC ACTGGACGAC AGAATTCGGC AGCCGCAGCA 
GCAGCCGCAG CAGCTCAAAA CGCTGCCAGT GGTGGTCTTC CACCGCATCC CGGCATGATG 
CACAAGGATG AGCCTGGCAT GGGATCGGCG GCGCAACAAC AGCAGCAGCA ACAACAGCAG 
CAGATGCAAA TTGCACAGCA GCAGCAGCAG GCCATTCAGC AGCATCATCA GCACCTGCAG 
CAGCAGCACC AGGCCCAGCA ACAGCAGCAG CAGCAACAAC ACCAGCAGCA GCAGCAACAA 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAACAAC AGCAAAAGTT AAAGCAATCG 
CAGGCGGCTA GTGCGGGGGC AAATAATAAG GCCACCAACC TGACGAAGGA TTCTCCCAAA 
CAAAAGGGCG GCGATGACGA TCAACCGCTG AAGGTGAAGC AGGAGGGCCA AAAGCCGACC 
ATGGAGACGC AGGGTCCGCC ACCACCACCC ACGTCGCAGT ACTTCCTTCA CCCCTCATAC 
ATTTCCCCGA CGCCCTTTGG GTTTGATCCC AATCATCCGA TGTATCGCAA CGTGTTGATG 
TCAGCCGCCG GACCGTATAA TACGGCACCG TACCACCTGC CCATCCCTCG CCCGTACCAT 
GCCCCAGAAG ATCTCTCGCG TAACACCGGC ACCAAGGCGT TGGATGCACT CCATCACGCG 
GCCAGTCAGT ACTACACCAC CCACAAGATC CATGAGCTCA GCGAACGGGC CCTCAAGTCG 
CCCACCAGCG GCAGCGGCCC CGTCAAGGTG AGCGTCAGCA GTCCCAGCAT CGGGCCGCCT 
CAACCAGGCG GACCCACGAG CAGCGGCCCC GGATCCGGAC CAGTTTCCGG TGTCCTCGGC 
CCCGGCAGCG GTTCCAACCA GCAGCCCGGC TCGGCACCTG GGTCTGCGGG CGGTGTGCCG 
CTGAACCTAC AGCCACCACC CGGAGGAATG GGCCCGTCAC CCGGCAGCAA GCCGGATCTG 
TCCGGCCCGA AAGGACATGG CGGAGTGACG CCCGGCTCGT CATTGGACGG CCACAAGCAG 
TCGATGCCCG GAGGTCCGCC ACCGAACGGG CCATCGGGCA ATGGAGCCGT TGGCGGTGTG 
GGAGGCGCTG CCGGCAATGG CGCAGCGGGA GGCGGCGGTG CAGGTGCCGC CGACTCACGC 
AGCCCACCAC CGCAGCGCCA TGTGCACACC CACCACCACA CGCACGTCGG CCTCGGCTAT 
CCCATGTACC CGGCGCCGTA TGGAGCGGCT GTTTTGGCTA GCCAGCAGGC GGCTGCTGTA 
GCTGTGATAA ACCCGTTTCC GCCGGGTCCG TCGAAATGAG ATCCACTGAA CGGTAACAAC 
GAGAAGAAGG CGTATAACGT AAAGAACAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sbb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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