SF1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SF1
Entrez Gene ID	43912
Full Name	Splicing factor 1
Synonyms	CG5836,Dmel\CG5836,cg5836,dSF1,p70
General protein information	Preferred Names Splicing factor 1 Names CG5836 gene product from transcript CG5836-RA CG5836-PA CG5836-PB CG5836-PC SF1-PA SF1-PB SF1-PC splicing factor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90B4-90B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275743.2	NP_001262672.1	splicing factor 1, isoform B
NM_001275744.1	NP_001262673.1	splicing factor 1, isoform C
NM_079915.3	NP_524654.2	splicing factor 1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The studies performed did not confirm the ability of the SF1 insulator to protect expression of reporter gene white from the chromosome position effect in transgenic lines.
Title: [Organization of the Drosophila melanogaster SF1 insulator and its role in transcription regulation in transgenic lines].

The following SF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07761	NM_001275744.1 Latest version!	Drosophila melanogaster splicing factor 1, transcript variant C (SF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa07762	NM_001275743.2 Latest version!	Drosophila melanogaster splicing factor 1, transcript variant B (SF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa06821	NM_079915.3 Latest version!	Drosophila melanogaster splicing factor 1, transcript variant A (SF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SF1 ( NM_001275744.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262673.1 Drosophila melanogaster splicing factor 1, transcript variant C (SF1), mRNA.

Price & Availability↑

CloneID	OFa07761
Clone ID Related Accession (Same CDS sequence)	NM_001275744.1
Accession Version	NM_001275744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGCCA CGCCGCCGGA CCTACTTTCG TTGGCGGCGT CCCGAGAAGT CTCGGCCGCC 
GTCGCAGTCG CCGCCTCTGC CAACGGAGGC AGCGGCAGAA GCGCTGATAA GGAGCGTTCA 
CGTTCCAGAG ATCGTGGGCG GGACAAGGAT AAGGATCGGG ACAAGGACAG GAAGAAGAGG 
GACAAAGACA GTAGGGACCG CGACAGTCGC CGGCGGGACG ATCGCGGCGA CCGAGATCGT 
GACCGGGAAC GCGACAGGGA TTCCCGCTCG CACCGCAGCA AGTCGCGCAA TTACGACGAC 
GGAGACAGAC GAAGAAAGCG ATCGCGCAGC AGGGATCGTG ATCGTGACCG CAAGCGGGAC 
AGAGATCGCG ACTCCCGCTC CAGGCGCAGT AGCTCGCGGG GTGGACGTGA TGATTATGAC 
CGGAGTCGTC GACGGCGCTC ACGCAGTCGC AGTTACGAGC GTCGACGCAA TGATCGAGGC 
AGTCAGAGAG ATCAACGAGA CCAGCATTCG CAAAGAAGCA GTCGGGAACG CACACATGTG 
AACCCCTTCG ACACATCCAA CAGTCGAAAC TCTCTGAATC AGGATCACGA TCAATCCAGA 
ATCCCGTCGC TCTTCGACCG ACAGCAGGGA TTGGAAACCA TCAGGGAGGA AGGACGTGAG 
CAGCGGTTTG ATCTTACTCA GACCATCCAG GAGCTAATGG GCAATGCTGG AGGCAACAAA 
GGATTCGCCT CGTTCTTTAA CAGCCAGAAC AGCAACGACT CCACCAGCAA TGGAGCATTC 
GATAACTCAG CGGACAGCGC TGCGGAGCGA AAGAGGAAGC GGAAGTCTCG CTGGGGCGGC 
AGTGAAAACG ACAAGACCTT CATTCCTGGA ATGCCCACAA TTCTGCCCTC CACCCTGGAC 
CCGGCACAGC AGGAGGCCTA CCTAGATCGC CCTCGCCGGA GCCCATTTAT AGTTCTGATG 
GCAAGCGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262673.1
CDS	205..1176
Translation	MSATPPDLLSLAASREVSAAVAVAASANGGSGRSADKERSRSRDRGRDKDKDRDKDRKKRDKDSRDRDSRRRDDRGDRDRDRERDRDSRSHRSKSRNYDDGDRRRKRSRSRDRDRDRKRDRDRDSRSRRSSSRGGRDDYDRSRRRRSRSRSYERRRNDRGSQRDQRDQHSQRSSRERTHVNPFDTSNSRNSLNQDHDQSRIPSLFDRQQGLETIREEGREQRFDLTQTIQELMGNAGGNKGFASFFNSQNSNDSTSNGAFDNSADSAAERKRKRKSRWGGSENDKTFIPGMPTILPSTLDPAQQEAYLDRPRRSPFIVLMASG

Target ORF information:

RefSeq Version	NM_001275744.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 1, transcript variant C (SF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275744.1

ORF Insert Sequence:

ATGAGCGCCA CGCCGCCGGA CCTACTTTCG TTGGCGGCGT CCCGAGAAGT CTCGGCCGCC 
GTCGCAGTCG CCGCCTCTGC CAACGGAGGC AGCGGCAGAA GCGCTGATAA GGAGCGTTCA 
CGTTCCAGAG ATCGTGGGCG GGACAAGGAT AAGGATCGGG ACAAGGACAG GAAGAAGAGG 
GACAAAGACA GTAGGGACCG CGACAGTCGC CGGCGGGACG ATCGCGGCGA CCGAGATCGT 
GACCGGGAAC GCGACAGGGA TTCCCGCTCG CACCGCAGCA AGTCGCGCAA TTACGACGAC 
GGAGACAGAC GAAGAAAGCG ATCGCGCAGC AGGGATCGTG ATCGTGACCG CAAGCGGGAC 
AGAGATCGCG ACTCCCGCTC CAGGCGCAGT AGCTCGCGGG GTGGACGTGA TGATTATGAC 
CGGAGTCGTC GACGGCGCTC ACGCAGTCGC AGTTACGAGC GTCGACGCAA TGATCGAGGC 
AGTCAGAGAG ATCAACGAGA CCAGCATTCG CAAAGAAGCA GTCGGGAACG CACACATGTG 
AACCCCTTCG ACACATCCAA CAGTCGAAAC TCTCTGAATC AGGATCACGA TCAATCCAGA 
ATCCCGTCGC TCTTCGACCG ACAGCAGGGA TTGGAAACCA TCAGGGAGGA AGGACGTGAG 
CAGCGGTTTG ATCTTACTCA GACCATCCAG GAGCTAATGG GCAATGCTGG AGGCAACAAA 
GGATTCGCCT CGTTCTTTAA CAGCCAGAAC AGCAACGACT CCACCAGCAA TGGAGCATTC 
GATAACTCAG CGGACAGCGC TGCGGAGCGA AAGAGGAAGC GGAAGTCTCG CTGGGGCGGC 
AGTGAAAACG ACAAGACCTT CATTCCTGGA ATGCCCACAA TTCTGCCCTC CACCCTGGAC 
CCGGCACAGC AGGAGGCCTA CCTAGATCGC CCTCGCCGGA GCCCATTTAT AGTTCTGATG 
GCAAGCGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF1 ( NM_001275743.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262672.1 Drosophila melanogaster splicing factor 1, transcript variant B (SF1), mRNA.

Price & Availability↑

CloneID	OFa07762
Clone ID Related Accession (Same CDS sequence)	NM_001275743.2
Accession Version	NM_001275743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275743.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGCCA CGCCGCCGGA CCTACTTTCG TTGGCGGCGT CCCGAGAAGT CTCGGCCGCC 
GTCGCAGTCG CCGCCTCTGC CAACGGAGGC AGCGGCAGAA GCGCTGATAA GGAGCGTTCA 
CGTTCCAGAG ATCGTGGGCG GGACAAGGAT AAGGATCGGG ACAAGGACAG GAAGAAGAGG 
GACAAAGACA GTAGGGACCG CGACAGTCGC CGGCGGGACG ATCGCGGCGA CCGAGATCGT 
GACCGGGAAC GCGACAGGGA TTCCCGCTCG CACCGCAGCA AGTCGCGCAA TTACGACGAC 
GGAGACAGAC GAAGAAAGCG ATCGCGCAGC AGGGATCGTG ATCGTGACCG CAAGCGGGAC 
AGAGATCGCG ACTCCCGCTC CAGGCGCAGT AGCTCGCGGG GTGGACGTGA TGATTATGAC 
CGGAGTCGTC GACGGCGCTC ACGCAGTCGC AGTTACGAGC GTCGACGCAA TGATCGAGGC 
AGTCAGAGAG ATCAACGAGA CCAGCATTCG CAAAGAAGCA GTCGGGAACG CACACATGTG 
AACCCCTTCG ACACATCCAA CAGTCGAAAC TCTCTGAATC AGGATCACGA TCAATCCAGA 
ATCCCGTCGC TCTTCGACCG ACAGCAGGGA TTGGAAACCA TCAGGGAGGA AGGACGTGAG 
CAGCGGTTTG ATCTTACTCA GACCATCCAG GAGCTAATGG GCAATGCTGG AGGCAACAAA 
GGATTCGCCT CGTTCTTTAA CAGCCAGAAC AGCAACGACT CCACCAGCAA TGGAGCATTC 
GATAACTCAG CGGACAGCGC TGCGGAGCGA AAGAGGAAGC GGAAGTCTCG CTGGGGCGGC 
AGTGAAAACG ACAAGACCTT CATTCCTGGA ATGCCCACAA TTCTGCCCTC CACCCTGGAC 
CCGGCACAGC AGGAGGCCTA CCTAGTTCAA TTTCAAATCG AGGAGATTAG TCGCAAGCTG 
CGAACCGGCG ATCTGGGCAT CACGCAGAAT CCGGAAGAAA GGTTTGATAC AGTTCTCTCT 
ACCCCACTGA GTCTCTCAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262672.1
CDS	205..1248
Translation	MSATPPDLLSLAASREVSAAVAVAASANGGSGRSADKERSRSRDRGRDKDKDRDKDRKKRDKDSRDRDSRRRDDRGDRDRDRERDRDSRSHRSKSRNYDDGDRRRKRSRSRDRDRDRKRDRDRDSRSRRSSSRGGRDDYDRSRRRRSRSRSYERRRNDRGSQRDQRDQHSQRSSRERTHVNPFDTSNSRNSLNQDHDQSRIPSLFDRQQGLETIREEGREQRFDLTQTIQELMGNAGGNKGFASFFNSQNSNDSTSNGAFDNSADSAAERKRKRKSRWGGSENDKTFIPGMPTILPSTLDPAQQEAYLVQFQIEEISRKLRTGDLGITQNPEERFDTVLSTPLSLSI

Target ORF information:

RefSeq Version	NM_001275743.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 1, transcript variant B (SF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275743.2

ORF Insert Sequence:

ATGAGCGCCA CGCCGCCGGA CCTACTTTCG TTGGCGGCGT CCCGAGAAGT CTCGGCCGCC 
GTCGCAGTCG CCGCCTCTGC CAACGGAGGC AGCGGCAGAA GCGCTGATAA GGAGCGTTCA 
CGTTCCAGAG ATCGTGGGCG GGACAAGGAT AAGGATCGGG ACAAGGACAG GAAGAAGAGG 
GACAAAGACA GTAGGGACCG CGACAGTCGC CGGCGGGACG ATCGCGGCGA CCGAGATCGT 
GACCGGGAAC GCGACAGGGA TTCCCGCTCG CACCGCAGCA AGTCGCGCAA TTACGACGAC 
GGAGACAGAC GAAGAAAGCG ATCGCGCAGC AGGGATCGTG ATCGTGACCG CAAGCGGGAC 
AGAGATCGCG ACTCCCGCTC CAGGCGCAGT AGCTCGCGGG GTGGACGTGA TGATTATGAC 
CGGAGTCGTC GACGGCGCTC ACGCAGTCGC AGTTACGAGC GTCGACGCAA TGATCGAGGC 
AGTCAGAGAG ATCAACGAGA CCAGCATTCG CAAAGAAGCA GTCGGGAACG CACACATGTG 
AACCCCTTCG ACACATCCAA CAGTCGAAAC TCTCTGAATC AGGATCACGA TCAATCCAGA 
ATCCCGTCGC TCTTCGACCG ACAGCAGGGA TTGGAAACCA TCAGGGAGGA AGGACGTGAG 
CAGCGGTTTG ATCTTACTCA GACCATCCAG GAGCTAATGG GCAATGCTGG AGGCAACAAA 
GGATTCGCCT CGTTCTTTAA CAGCCAGAAC AGCAACGACT CCACCAGCAA TGGAGCATTC 
GATAACTCAG CGGACAGCGC TGCGGAGCGA AAGAGGAAGC GGAAGTCTCG CTGGGGCGGC 
AGTGAAAACG ACAAGACCTT CATTCCTGGA ATGCCCACAA TTCTGCCCTC CACCCTGGAC 
CCGGCACAGC AGGAGGCCTA CCTAGTTCAA TTTCAAATCG AGGAGATTAG TCGCAAGCTG 
CGAACCGGCG ATCTGGGCAT CACGCAGAAT CCGGAAGAAA GGTTTGATAC AGTTCTCTCT 
ACCCCACTGA GTCTCTCAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SF1 ( NM_079915.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524654.2 Drosophila melanogaster splicing factor 1, transcript variant A (SF1), mRNA.

Price & Availability↑

CloneID	OFa06821
Clone ID Related Accession (Same CDS sequence)	NM_079915.3
Accession Version	NM_079915.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	splicing factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079915.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGCCA CGCCGCCGGA CCTACTTTCG TTGGCGGCGT CCCGAGAAGT CTCGGCCGCC 
GTCGCAGTCG CCGCCTCTGC CAACGGAGGC AGCGGCAGAA GCGCTGATAA GGAGCGTTCA 
CGTTCCAGAG ATCGTGGGCG GGACAAGGAT AAGGATCGGG ACAAGGACAG GAAGAAGAGG 
GACAAAGACA GTAGGGACCG CGACAGTCGC CGGCGGGACG ATCGCGGCGA CCGAGATCGT 
GACCGGGAAC GCGACAGGGA TTCCCGCTCG CACCGCAGCA AGTCGCGCAA TTACGACGAC 
GGAGACAGAC GAAGAAAGCG ATCGCGCAGC AGGGATCGTG ATCGTGACCG CAAGCGGGAC 
AGAGATCGCG ACTCCCGCTC CAGGCGCAGT AGCTCGCGGG GTGGACGTGA TGATTATGAC 
CGGAGTCGTC GACGGCGCTC ACGCAGTCGC AGTTACGAGC GTCGACGCAA TGATCGAGGC 
AGTCAGAGAG ATCAACGAGA CCAGCATTCG CAAAGAAGCA GTCGGGAACG CACACATGTG 
AACCCCTTCG ACACATCCAA CAGTCGAAAC TCTCTGAATC AGGATCACGA TCAATCCAGA 
ATCCCGTCGC TCTTCGACCG ACAGCAGGGA TTGGAAACCA TCAGGGAGGA AGGACGTGAG 
CAGCGGTTTG ATCTTACTCA GACCATCCAG GAGCTAATGG GCAATGCTGG AGGCAACAAA 
GGATTCGCCT CGTTCTTTAA CAGCCAGAAC AGCAACGACT CCACCAGCAA TGGAGCATTC 
GATAACTCAG CGGACAGCGC TGCGGAGCGA AAGAGGAAGC GGAAGTCTCG CTGGGGCGGC 
AGTGAAAACG ACAAGACCTT CATTCCTGGA ATGCCCACAA TTCTGCCCTC CACCCTGGAC 
CCGGCACAGC AGGAGGCCTA CCTAGTTCAA TTTCAAATCG AGGAGATTAG TCGCAAGCTG 
CGAACCGGCG ATCTGGGCAT CACGCAGAAT CCGGAAGAAA GATCGCCCTC GCCGGAGCCC 
ATTTATAGTT CTGATGGCAA GCGGTTGAAC ACCCGTGAAT TCCGCTACAG GAAAAGACTG 
GAAGAGCAGC GTCATCAACT GATCGTCAAA ATGCAGACGG TCAACCCAGA GTTCAAGCCA 
CCAGCGGATT ACAAACCGCC AGTCACTCGT GTCAGCGACA AAGTGCTGAT TCCACAGGAG 
CAGCACCCTG ACATCAATTT TGTGGGCCTG CTCATCGGGC CGCGAGGCAA CACGCTCAAG 
GCTATGGAGA AGGATACGGG CGCCAAGATC ATAATTCGCG GCAAGGGATC GGTGAAGGAG 
GGCAAAGTGG GCCGAAAGGA TGGCCAACCG TTGCCGGGCG AGGATGAACC ACTGCATGCT 
TTCATTACCG CCCCCAATCC AGAGGCGGTG CGCAAGGCAG TGGACAAAAT CAAAGACGTC 
ATCCGTCAGG GTATCGAAGT GCCCGAGGGC CATAATGATC TCCGCAGAAT GCAGTTGCGA 
GAACTGGCTC AGCTGAATGG AACACTTCGA GAGAACGATA TCCAACGCTG TACGTGCGGC 
TCAACGGATC ACAAGTCCTG GCAATGTCCG GATAAGCCGA TCATCACGAA CACCATAGTG 
TGCACCTCAT GTGGCGGTAC CGGTCATTTG ACCAAGGATT GCCGCAACAA GCGTCCAGGA 
TCCGGAGTAC CCGGCATGGC CTGCGAAGAT TCCCAGGCTA AGATTGACGA GGAGTATATG 
AGTTTGATGG CCGAATTGGG AGAAGGACCA CCGCCGCCAT CCGCTTCTGC TAAGACCGAT 
CCGCCGGCCA GTAATGGACC GCAGTTGCAC CGGGCCAGCT ATAGCATTTT TGACAAGAAG 
CCCTCCCAGA TGCAGGCCAT CCAGTCGCCA CCGAGCTCCT CGTCGCGAGA TCACCAGCGC 
GACCTGGGCG GATGGGGAGC GGCGGCGCAC GAACACGGCA TGGGCATGGA GCACGGTATG 
GGATTGGACC AGCACGGTCA CGGTTTGGCA GCGATGGATC ACGGCATGAG CCTTGGCATG 
GAGCACGCAA TGGCGGCCTA TGTGCCGGCA CCGCCGGGCA CTCAAGCACC GCCTCCGATG 
CCGCCGCCGC TGATGCCGTG GATGAGCGCA CCGCAGCCTC CGCCGCCGGC AACAGAACCG 
CTGAATCCAC CTATACCGGG CACCTTGCCA CCGCTAATCC CGCCGCCACC CGGCACCAGT 
GCCCCGCCCA TGCCGCCGTG GGGTGGTGGC GCTTACAGTG GCTGGGGCGG CGGCTATGCG 
CCGCCTCCAC CTCCGCCCTG CGCACCGCCG CCACCAGCCT TGAGCCTGTC GCAGCCGCCG 
CCACCTCCAC CGCCATCCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524654.2
CDS	205..2568
Translation	MSATPPDLLSLAASREVSAAVAVAASANGGSGRSADKERSRSRDRGRDKDKDRDKDRKKRDKDSRDRDSRRRDDRGDRDRDRERDRDSRSHRSKSRNYDDGDRRRKRSRSRDRDRDRKRDRDRDSRSRRSSSRGGRDDYDRSRRRRSRSRSYERRRNDRGSQRDQRDQHSQRSSRERTHVNPFDTSNSRNSLNQDHDQSRIPSLFDRQQGLETIREEGREQRFDLTQTIQELMGNAGGNKGFASFFNSQNSNDSTSNGAFDNSADSAAERKRKRKSRWGGSENDKTFIPGMPTILPSTLDPAQQEAYLVQFQIEEISRKLRTGDLGITQNPEERSPSPEPIYSSDGKRLNTREFRYRKRLEEQRHQLIVKMQTVNPEFKPPADYKPPVTRVSDKVLIPQEQHPDINFVGLLIGPRGNTLKAMEKDTGAKIIIRGKGSVKEGKVGRKDGQPLPGEDEPLHAFITAPNPEAVRKAVDKIKDVIRQGIEVPEGHNDLRRMQLRELAQLNGTLRENDIQRCTCGSTDHKSWQCPDKPIITNTIVCTSCGGTGHLTKDCRNKRPGSGVPGMACEDSQAKIDEEYMSLMAELGEGPPPPSASAKTDPPASNGPQLHRASYSIFDKKPSQMQAIQSPPSSSSRDHQRDLGGWGAAAHEHGMGMEHGMGLDQHGHGLAAMDHGMSLGMEHAMAAYVPAPPGTQAPPPMPPPLMPWMSAPQPPPPATEPLNPPIPGTLPPLIPPPPGTSAPPMPPWGGGAYSGWGGGYAPPPPPPCAPPPPALSLSQPPPPPPPSA

Target ORF information:

RefSeq Version	NM_079915.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster splicing factor 1, transcript variant A (SF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079915.3

ORF Insert Sequence:

ATGAGCGCCA CGCCGCCGGA CCTACTTTCG TTGGCGGCGT CCCGAGAAGT CTCGGCCGCC 
GTCGCAGTCG CCGCCTCTGC CAACGGAGGC AGCGGCAGAA GCGCTGATAA GGAGCGTTCA 
CGTTCCAGAG ATCGTGGGCG GGACAAGGAT AAGGATCGGG ACAAGGACAG GAAGAAGAGG 
GACAAAGACA GTAGGGACCG CGACAGTCGC CGGCGGGACG ATCGCGGCGA CCGAGATCGT 
GACCGGGAAC GCGACAGGGA TTCCCGCTCG CACCGCAGCA AGTCGCGCAA TTACGACGAC 
GGAGACAGAC GAAGAAAGCG ATCGCGCAGC AGGGATCGTG ATCGTGACCG CAAGCGGGAC 
AGAGATCGCG ACTCCCGCTC CAGGCGCAGT AGCTCGCGGG GTGGACGTGA TGATTATGAC 
CGGAGTCGTC GACGGCGCTC ACGCAGTCGC AGTTACGAGC GTCGACGCAA TGATCGAGGC 
AGTCAGAGAG ATCAACGAGA CCAGCATTCG CAAAGAAGCA GTCGGGAACG CACACATGTG 
AACCCCTTCG ACACATCCAA CAGTCGAAAC TCTCTGAATC AGGATCACGA TCAATCCAGA 
ATCCCGTCGC TCTTCGACCG ACAGCAGGGA TTGGAAACCA TCAGGGAGGA AGGACGTGAG 
CAGCGGTTTG ATCTTACTCA GACCATCCAG GAGCTAATGG GCAATGCTGG AGGCAACAAA 
GGATTCGCCT CGTTCTTTAA CAGCCAGAAC AGCAACGACT CCACCAGCAA TGGAGCATTC 
GATAACTCAG CGGACAGCGC TGCGGAGCGA AAGAGGAAGC GGAAGTCTCG CTGGGGCGGC 
AGTGAAAACG ACAAGACCTT CATTCCTGGA ATGCCCACAA TTCTGCCCTC CACCCTGGAC 
CCGGCACAGC AGGAGGCCTA CCTAGTTCAA TTTCAAATCG AGGAGATTAG TCGCAAGCTG 
CGAACCGGCG ATCTGGGCAT CACGCAGAAT CCGGAAGAAA GATCGCCCTC GCCGGAGCCC 
ATTTATAGTT CTGATGGCAA GCGGTTGAAC ACCCGTGAAT TCCGCTACAG GAAAAGACTG 
GAAGAGCAGC GTCATCAACT GATCGTCAAA ATGCAGACGG TCAACCCAGA GTTCAAGCCA 
CCAGCGGATT ACAAACCGCC AGTCACTCGT GTCAGCGACA AAGTGCTGAT TCCACAGGAG 
CAGCACCCTG ACATCAATTT TGTGGGCCTG CTCATCGGGC CGCGAGGCAA CACGCTCAAG 
GCTATGGAGA AGGATACGGG CGCCAAGATC ATAATTCGCG GCAAGGGATC GGTGAAGGAG 
GGCAAAGTGG GCCGAAAGGA TGGCCAACCG TTGCCGGGCG AGGATGAACC ACTGCATGCT 
TTCATTACCG CCCCCAATCC AGAGGCGGTG CGCAAGGCAG TGGACAAAAT CAAAGACGTC 
ATCCGTCAGG GTATCGAAGT GCCCGAGGGC CATAATGATC TCCGCAGAAT GCAGTTGCGA 
GAACTGGCTC AGCTGAATGG AACACTTCGA GAGAACGATA TCCAACGCTG TACGTGCGGC 
TCAACGGATC ACAAGTCCTG GCAATGTCCG GATAAGCCGA TCATCACGAA CACCATAGTG 
TGCACCTCAT GTGGCGGTAC CGGTCATTTG ACCAAGGATT GCCGCAACAA GCGTCCAGGA 
TCCGGAGTAC CCGGCATGGC CTGCGAAGAT TCCCAGGCTA AGATTGACGA GGAGTATATG 
AGTTTGATGG CCGAATTGGG AGAAGGACCA CCGCCGCCAT CCGCTTCTGC TAAGACCGAT 
CCGCCGGCCA GTAATGGACC GCAGTTGCAC CGGGCCAGCT ATAGCATTTT TGACAAGAAG 
CCCTCCCAGA TGCAGGCCAT CCAGTCGCCA CCGAGCTCCT CGTCGCGAGA TCACCAGCGC 
GACCTGGGCG GATGGGGAGC GGCGGCGCAC GAACACGGCA TGGGCATGGA GCACGGTATG 
GGATTGGACC AGCACGGTCA CGGTTTGGCA GCGATGGATC ACGGCATGAG CCTTGGCATG 
GAGCACGCAA TGGCGGCCTA TGTGCCGGCA CCGCCGGGCA CTCAAGCACC GCCTCCGATG 
CCGCCGCCGC TGATGCCGTG GATGAGCGCA CCGCAGCCTC CGCCGCCGGC AACAGAACCG 
CTGAATCCAC CTATACCGGG CACCTTGCCA CCGCTAATCC CGCCGCCACC CGGCACCAGT 
GCCCCGCCCA TGCCGCCGTG GGGTGGTGGC GCTTACAGTG GCTGGGGCGG CGGCTATGCG 
CCGCCTCCAC CTCCGCCCTG CGCACCGCCG CCACCAGCCT TGAGCCTGTC GCAGCCGCCG 
CCACCTCCAC CGCCATCCGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SF1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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