E23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	E23
Entrez Gene ID	43895
Full Name	Early gene at 23
Synonyms	CG15410,CG3327,Dmel\CG3327
General protein information	Preferred Names Early gene at 23 Names CG3327 gene product from transcript CG3327-RD CG3327-PC CG3327-PD CG3327-PE E23-PC E23-PD E23-PE early gene at 23
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23E4-23E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_205900.2	NP_995622.1	early gene at 23, isoform C
NM_079909.3	NP_524648.3	early gene at 23, isoform D
NM_134924.2	NP_608768.2	early gene at 23, isoform E

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os11g0177400	NP_001065886.1
Drosophila melanogaster (fruit fly)	E23	NP_524648.2
Arabidopsis thaliana (thale cress)	ABCG25	NP_565030.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

E23 forms its own feedback loop in the ecdysone response to control circadian oscillation through ecdysone-mediated vrille expression.
Title: Membrane-bound transporter controls the circadian transcription of clock genes in Drosophila.

The following E23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa15178	NM_134924.2 Latest version!	Drosophila melanogaster early gene at 23, transcript variant E (E23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa15178	NM_205900.2 Latest version!	Drosophila melanogaster early gene at 23, transcript variant C (E23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa15178	NM_079909.3 Latest version!	Drosophila melanogaster early gene at 23, transcript variant D (E23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

E23 ( NM_205900.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995622.1 Drosophila melanogaster early gene at 23, transcript variant C (E23), mRNA.

Price & Availability↑

CloneID	OFa15178
Clone ID Related Accession (Same CDS sequence)	NM_205900.2 , NM_079909.3 , NM_134924.2
Accession Version	NM_205900.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	early gene at 23, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205900.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATTT CCAAGGATCT TTTCATATTT GGCGAACAAA TTTTTGCAAC AATCATACCA 
GAAGGTGGAG CCGCGGCGCT GGCAACCCAG CAGCAACATC AGCAGCAGCA TCAGCAGCAG 
CATCAGCAGT CGCATCAGCA GCCGCATCAG CAGTCGCAGC AGCAGCAGCA GCAACAACAG 
CCGGCAATTA TAAATTCAAC AACGACGACG GCCACTTCGT TGGTAATAAA CTCGACAGCA 
GGAGCAGCTG AACAAGTTGT AGAGGAGTCA TTAAAAGTAG CAGCAGCAGC AGCAGCAGCA 
ACAACAACAA CAACAGCCGC AGCAGCAACA ACACTACCAG CTAAACAGGA AACAGTTGCA 
GTTGCTGCCG CCTGTGTTGC TCCTCGCAAA TTGGCAAAAA TTCAGAAAAT TCCAAAGGAA 
CCCGAGGAGC TCAAAGCCAA GAAAAAGGCA AAGTGTGAGG CAGCAGCAGC GGCGACTCAT 
TACGAAAAAA TGCCAATGAA TTTGATGATC GTGGAAAGTG TTGAGGCAGT CGATATGGGA 
TCATCGGACA ATGACAACGA GACAACGGAG CAGGTACCGT TGGTGAGTGC CAGCCGTCGC 
AATCTGGCCA AAATAGTGGC CGACTGCCGC CTCCAGATCG AAGTGGAGCC GGCAACATCG 
GCAGTGGCCG CAGCAGCAGC AGCAGCAGCA AGCGTTGCAT CCCCGCCTGC AACATCGGAT 
GCGACAGCAG CGACATCGGT GGCCAGCAGC GACTCCTCTG CCTCATCATC AGCATCTTCG 
ACGTCGTCGT CATCTTCCAG CTCAACGTCC TCAGAATTTA CGCACACATC ACAGACAACG 
ATACCAAGTA CGGATAATCT AACGTCATCC AATGGTCTGG CCAATATAGG CAAAAGCATT 
TTAGTTGATG CCAAAAGTAA CTCCCCAGCT TCCTTGTCTT ACCACTTTGA GAAGTCTGCG 
GCGGCGAACC AACAGAAGCA GCATCACAAC ATAAACATCT GCAACAGCCA CCACCCACAT 
CACCACTCCC AGAATCAGCC CCAGCACAGT TTGCAGCTGA CCTTCCAGAA CCTGAACGTT 
TTGCACAACG AACGCCAAAT ACTTTCCGAT GTGAGTGGAT TCGTCAGTCC CTGCGAGGTC 
CTGGCCATCA TGGGTCCTTC GGGCAGCGGG AAGACCACTC TGCTGGACTG CCTCAGTGGT 
CAGCGGCACA TTGACAGCGG CAGTGTGTTC CTCAACCGTG AGCCCCTGAC CAAAAAGTGG 
CGTCGAAGGA TCGGCTATGT CCTCCAGGAG GAGATCTTCT TCCCGCAATT GACGCTCCGC 
GAAACGGTGG TCTATACTGC GCTGCTGCGA CTGCCGGAAT CAATGCCGCG GGCGGAGAAG 
ATGCGCCAGG TGGACCACAT ACTGGAGGCT CTGGAATTGG GTTGCTGTCA GCAGACCAAG 
TTCGGGGACT ACCTCAACCG TGGACTCTCC GGTGGCGAGA AGAAGCGGGC GAATATCGCC 
TGTGAACTGC TGACCAATCC GCTGCTCATG CTCCTGGATG AACCCACTTC TGGACTGGAC 
AGCCACTCGG CCATTTCGCT GATGAAAGTA CTCAAGCGGT ACGCCCAGCT GGAGCAGAAG 
ACCATCGTGA TCAGCGTGCA CCAGCCTTCG TCCCAGATGT TCCACATGTT CGACAAGCTG 
CTTCTGCTCC ACCAAGGACG CACTGCGTAC TTTGGGGACG TGCAAAACAT CTACCGCCAC 
TTCGAGGACA TCGGGGTCAC CATCAAGCCG CACTACAATC CCGCCGATTT TGTGTTGGAG 
CAACTGAAAT CGCATCCGGA TATCCGCGAG AAACTCTTCA TAGCCGCCAA GGAATCGCAC 
GGCAACTATC TGAACCGCAA CTGCATCACG AGTAGCCACC ATAACCAGGT CAGCGTTTCG 
GGTGCGAAGG GCAAGAAGCA AGCGGATAGC ATCCTGATCG ACGACATAAT AAATAACTAT 
TACAATCAAC GCTCCAACCG GCACCATCAC CAGTACGAGA ACCTGCACCA CACGAGCAAC 
GGCTGCCGGG TGGAGGAGGA CGAGGAGGCG GCCCAGCACC TGGTGTGGTG TGCCGCGGAC 
TCGCAGTCCA ACTTCAGTTC CTGCGCCTCC AGCGATTGTC ATTCCTATAG TGCTGGCAGT 
GGGCCAGGAC ATTCGGCGGA TGACGACTGG CTGTCCTATC CCACCAGCTT CCACACCCAG 
TTCTGCGTCC TCTCAAGTCG CAATTTCCGG GAGGCTAAGC CGCGCATGCT GTCCAAACTC 
AATTGGTTCC AAACTATCGG ACTGGCTCTG ATGGCCGGTG CCATTTGGTT CCAGCTGCCC 
AGAACCGAGG AGTTCCTGCA CGACCTCCAG GGATGGATGT TCTTCTCGCA GACCTACTGG 
ATGCTCTTCG CCCTCTTCGG GGCCCTTAAT TCATTCCCCT CGGAGCGCGA GGTGGTGAGC 
AAGGAACGTC GCTCCGGGGC CTACCGACTA TCCGCCTATT ATTTGGCCAA AATGTGTGCC 
GAGCTGCCCT TGGTGATCAC TTTGCCCACG GTCTACCTCA TGATCAGCTA CCCCATGCTG 
GGCTGCACCA GCTTGAAGCT ATTCTTCCTG ATGCTCATCT TTCTGCTGAT CAACACGATC 
GTGGCCCAGA GCGTGGGCTT CTTCATCGGA GCCTGCTGCA TGGACATGAA CGTGAGCATC 
ACCCTGAGTG CCCTGTACAC GCTGGCCACC CAGCTGTTCG GCGGATATCT GTCTTCGCGC 
ATTCCGGAGG GGCTCAGCTG GATCCGGTAC ACCTCCATGA TCCACTACGC CTACCAGAAC 
ATGCAGATTC TCGAGTTCCG CGAGGGGGCG GCTATTGGTT GTGGCTCGCC CAGCAGTTAC 
GAGATTTGCA AACAGCAATC AACCGAATTC ATTCCATACG AGGAAATACT CAAAGCCCAA 
AACTCGACAT CACCGCTCTG GCTGAACACG CTGATCCTGA TGATGTTCTT CCTGGTATTC 
CGGTGCCTGG GATACGCGGT GTTGCGCTTC CTGCGATGCC CCAAAAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995622.1
CDS	679..3732
Translation	MIISKDLFIFGEQIFATIIPEGGAAALATQQQHQQQHQQQHQQSHQQPHQQSQQQQQQQQPAIINSTTTTATSLVINSTAGAAEQVVEESLKVAAAAAAATTTTTAAAATTLPAKQETVAVAAACVAPRKLAKIQKIPKEPEELKAKKKAKCEAAAAATHYEKMPMNLMIVESVEAVDMGSSDNDNETTEQVPLVSASRRNLAKIVADCRLQIEVEPATSAVAAAAAAAASVASPPATSDATAATSVASSDSSASSSASSTSSSSSSSTSSEFTHTSQTTIPSTDNLTSSNGLANIGKSILVDAKSNSPASLSYHFEKSAAANQQKQHHNINICNSHHPHHHSQNQPQHSLQLTFQNLNVLHNERQILSDVSGFVSPCEVLAIMGPSGSGKTTLLDCLSGQRHIDSGSVFLNREPLTKKWRRRIGYVLQEEIFFPQLTLRETVVYTALLRLPESMPRAEKMRQVDHILEALELGCCQQTKFGDYLNRGLSGGEKKRANIACELLTNPLLMLLDEPTSGLDSHSAISLMKVLKRYAQLEQKTIVISVHQPSSQMFHMFDKLLLLHQGRTAYFGDVQNIYRHFEDIGVTIKPHYNPADFVLEQLKSHPDIREKLFIAAKESHGNYLNRNCITSSHHNQVSVSGAKGKKQADSILIDDIINNYYNQRSNRHHHQYENLHHTSNGCRVEEDEEAAQHLVWCAADSQSNFSSCASSDCHSYSAGSGPGHSADDDWLSYPTSFHTQFCVLSSRNFREAKPRMLSKLNWFQTIGLALMAGAIWFQLPRTEEFLHDLQGWMFFSQTYWMLFALFGALNSFPSEREVVSKERRSGAYRLSAYYLAKMCAELPLVITLPTVYLMISYPMLGCTSLKLFFLMLIFLLINTIVAQSVGFFIGACCMDMNVSITLSALYTLATQLFGGYLSSRIPEGLSWIRYTSMIHYAYQNMQILEFREGAAIGCGSPSSYEICKQQSTEFIPYEEILKAQNSTSPLWLNTLILMMFFLVFRCLGYAVLRFLRCPKKT

Target ORF information:

RefSeq Version	NM_205900.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster early gene at 23, transcript variant C (E23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205900.2

ORF Insert Sequence:

ATGATTATTT CCAAGGATCT TTTCATATTT GGCGAACAAA TTTTTGCAAC AATCATACCA 
GAAGGTGGAG CCGCGGCGCT GGCAACCCAG CAGCAACATC AGCAGCAGCA TCAGCAGCAG 
CATCAGCAGT CGCATCAGCA GCCGCATCAG CAGTCGCAGC AGCAGCAGCA GCAACAACAG 
CCGGCAATTA TAAATTCAAC AACGACGACG GCCACTTCGT TGGTAATAAA CTCGACAGCA 
GGAGCAGCTG AACAAGTTGT AGAGGAGTCA TTAAAAGTAG CAGCAGCAGC AGCAGCAGCA 
ACAACAACAA CAACAGCCGC AGCAGCAACA ACACTACCAG CTAAACAGGA AACAGTTGCA 
GTTGCTGCCG CCTGTGTTGC TCCTCGCAAA TTGGCAAAAA TTCAGAAAAT TCCAAAGGAA 
CCCGAGGAGC TCAAAGCCAA GAAAAAGGCA AAGTGTGAGG CAGCAGCAGC GGCGACTCAT 
TACGAAAAAA TGCCAATGAA TTTGATGATC GTGGAAAGTG TTGAGGCAGT CGATATGGGA 
TCATCGGACA ATGACAACGA GACAACGGAG CAGGTACCGT TGGTGAGTGC CAGCCGTCGC 
AATCTGGCCA AAATAGTGGC CGACTGCCGC CTCCAGATCG AAGTGGAGCC GGCAACATCG 
GCAGTGGCCG CAGCAGCAGC AGCAGCAGCA AGCGTTGCAT CCCCGCCTGC AACATCGGAT 
GCGACAGCAG CGACATCGGT GGCCAGCAGC GACTCCTCTG CCTCATCATC AGCATCTTCG 
ACGTCGTCGT CATCTTCCAG CTCAACGTCC TCAGAATTTA CGCACACATC ACAGACAACG 
ATACCAAGTA CGGATAATCT AACGTCATCC AATGGTCTGG CCAATATAGG CAAAAGCATT 
TTAGTTGATG CCAAAAGTAA CTCCCCAGCT TCCTTGTCTT ACCACTTTGA GAAGTCTGCG 
GCGGCGAACC AACAGAAGCA GCATCACAAC ATAAACATCT GCAACAGCCA CCACCCACAT 
CACCACTCCC AGAATCAGCC CCAGCACAGT TTGCAGCTGA CCTTCCAGAA CCTGAACGTT 
TTGCACAACG AACGCCAAAT ACTTTCCGAT GTGAGTGGAT TCGTCAGTCC CTGCGAGGTC 
CTGGCCATCA TGGGTCCTTC GGGCAGCGGG AAGACCACTC TGCTGGACTG CCTCAGTGGT 
CAGCGGCACA TTGACAGCGG CAGTGTGTTC CTCAACCGTG AGCCCCTGAC CAAAAAGTGG 
CGTCGAAGGA TCGGCTATGT CCTCCAGGAG GAGATCTTCT TCCCGCAATT GACGCTCCGC 
GAAACGGTGG TCTATACTGC GCTGCTGCGA CTGCCGGAAT CAATGCCGCG GGCGGAGAAG 
ATGCGCCAGG TGGACCACAT ACTGGAGGCT CTGGAATTGG GTTGCTGTCA GCAGACCAAG 
TTCGGGGACT ACCTCAACCG TGGACTCTCC GGTGGCGAGA AGAAGCGGGC GAATATCGCC 
TGTGAACTGC TGACCAATCC GCTGCTCATG CTCCTGGATG AACCCACTTC TGGACTGGAC 
AGCCACTCGG CCATTTCGCT GATGAAAGTA CTCAAGCGGT ACGCCCAGCT GGAGCAGAAG 
ACCATCGTGA TCAGCGTGCA CCAGCCTTCG TCCCAGATGT TCCACATGTT CGACAAGCTG 
CTTCTGCTCC ACCAAGGACG CACTGCGTAC TTTGGGGACG TGCAAAACAT CTACCGCCAC 
TTCGAGGACA TCGGGGTCAC CATCAAGCCG CACTACAATC CCGCCGATTT TGTGTTGGAG 
CAACTGAAAT CGCATCCGGA TATCCGCGAG AAACTCTTCA TAGCCGCCAA GGAATCGCAC 
GGCAACTATC TGAACCGCAA CTGCATCACG AGTAGCCACC ATAACCAGGT CAGCGTTTCG 
GGTGCGAAGG GCAAGAAGCA AGCGGATAGC ATCCTGATCG ACGACATAAT AAATAACTAT 
TACAATCAAC GCTCCAACCG GCACCATCAC CAGTACGAGA ACCTGCACCA CACGAGCAAC 
GGCTGCCGGG TGGAGGAGGA CGAGGAGGCG GCCCAGCACC TGGTGTGGTG TGCCGCGGAC 
TCGCAGTCCA ACTTCAGTTC CTGCGCCTCC AGCGATTGTC ATTCCTATAG TGCTGGCAGT 
GGGCCAGGAC ATTCGGCGGA TGACGACTGG CTGTCCTATC CCACCAGCTT CCACACCCAG 
TTCTGCGTCC TCTCAAGTCG CAATTTCCGG GAGGCTAAGC CGCGCATGCT GTCCAAACTC 
AATTGGTTCC AAACTATCGG ACTGGCTCTG ATGGCCGGTG CCATTTGGTT CCAGCTGCCC 
AGAACCGAGG AGTTCCTGCA CGACCTCCAG GGATGGATGT TCTTCTCGCA GACCTACTGG 
ATGCTCTTCG CCCTCTTCGG GGCCCTTAAT TCATTCCCCT CGGAGCGCGA GGTGGTGAGC 
AAGGAACGTC GCTCCGGGGC CTACCGACTA TCCGCCTATT ATTTGGCCAA AATGTGTGCC 
GAGCTGCCCT TGGTGATCAC TTTGCCCACG GTCTACCTCA TGATCAGCTA CCCCATGCTG 
GGCTGCACCA GCTTGAAGCT ATTCTTCCTG ATGCTCATCT TTCTGCTGAT CAACACGATC 
GTGGCCCAGA GCGTGGGCTT CTTCATCGGA GCCTGCTGCA TGGACATGAA CGTGAGCATC 
ACCCTGAGTG CCCTGTACAC GCTGGCCACC CAGCTGTTCG GCGGATATCT GTCTTCGCGC 
ATTCCGGAGG GGCTCAGCTG GATCCGGTAC ACCTCCATGA TCCACTACGC CTACCAGAAC 
ATGCAGATTC TCGAGTTCCG CGAGGGGGCG GCTATTGGTT GTGGCTCGCC CAGCAGTTAC 
GAGATTTGCA AACAGCAATC AACCGAATTC ATTCCATACG AGGAAATACT CAAAGCCCAA 
AACTCGACAT CACCGCTCTG GCTGAACACG CTGATCCTGA TGATGTTCTT CCTGGTATTC 
CGGTGCCTGG GATACGCGGT GTTGCGCTTC CTGCGATGCC CCAAAAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E23 ( NM_079909.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524648.3 Drosophila melanogaster early gene at 23, transcript variant D (E23), mRNA.

Price & Availability↑

CloneID	OFa15178
Clone ID Related Accession (Same CDS sequence)	NM_205900.2 , NM_079909.3 , NM_134924.2
Accession Version	NM_079909.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	early gene at 23, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_079909.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATTT CCAAGGATCT TTTCATATTT GGCGAACAAA TTTTTGCAAC AATCATACCA 
GAAGGTGGAG CCGCGGCGCT GGCAACCCAG CAGCAACATC AGCAGCAGCA TCAGCAGCAG 
CATCAGCAGT CGCATCAGCA GCCGCATCAG CAGTCGCAGC AGCAGCAGCA GCAACAACAG 
CCGGCAATTA TAAATTCAAC AACGACGACG GCCACTTCGT TGGTAATAAA CTCGACAGCA 
GGAGCAGCTG AACAAGTTGT AGAGGAGTCA TTAAAAGTAG CAGCAGCAGC AGCAGCAGCA 
ACAACAACAA CAACAGCCGC AGCAGCAACA ACACTACCAG CTAAACAGGA AACAGTTGCA 
GTTGCTGCCG CCTGTGTTGC TCCTCGCAAA TTGGCAAAAA TTCAGAAAAT TCCAAAGGAA 
CCCGAGGAGC TCAAAGCCAA GAAAAAGGCA AAGTGTGAGG CAGCAGCAGC GGCGACTCAT 
TACGAAAAAA TGCCAATGAA TTTGATGATC GTGGAAAGTG TTGAGGCAGT CGATATGGGA 
TCATCGGACA ATGACAACGA GACAACGGAG CAGGTACCGT TGGTGAGTGC CAGCCGTCGC 
AATCTGGCCA AAATAGTGGC CGACTGCCGC CTCCAGATCG AAGTGGAGCC GGCAACATCG 
GCAGTGGCCG CAGCAGCAGC AGCAGCAGCA AGCGTTGCAT CCCCGCCTGC AACATCGGAT 
GCGACAGCAG CGACATCGGT GGCCAGCAGC GACTCCTCTG CCTCATCATC AGCATCTTCG 
ACGTCGTCGT CATCTTCCAG CTCAACGTCC TCAGAATTTA CGCACACATC ACAGACAACG 
ATACCAAGTA CGGATAATCT AACGTCATCC AATGGTCTGG CCAATATAGG CAAAAGCATT 
TTAGTTGATG CCAAAAGTAA CTCCCCAGCT TCCTTGTCTT ACCACTTTGA GAAGTCTGCG 
GCGGCGAACC AACAGAAGCA GCATCACAAC ATAAACATCT GCAACAGCCA CCACCCACAT 
CACCACTCCC AGAATCAGCC CCAGCACAGT TTGCAGCTGA CCTTCCAGAA CCTGAACGTT 
TTGCACAACG AACGCCAAAT ACTTTCCGAT GTGAGTGGAT TCGTCAGTCC CTGCGAGGTC 
CTGGCCATCA TGGGTCCTTC GGGCAGCGGG AAGACCACTC TGCTGGACTG CCTCAGTGGT 
CAGCGGCACA TTGACAGCGG CAGTGTGTTC CTCAACCGTG AGCCCCTGAC CAAAAAGTGG 
CGTCGAAGGA TCGGCTATGT CCTCCAGGAG GAGATCTTCT TCCCGCAATT GACGCTCCGC 
GAAACGGTGG TCTATACTGC GCTGCTGCGA CTGCCGGAAT CAATGCCGCG GGCGGAGAAG 
ATGCGCCAGG TGGACCACAT ACTGGAGGCT CTGGAATTGG GTTGCTGTCA GCAGACCAAG 
TTCGGGGACT ACCTCAACCG TGGACTCTCC GGTGGCGAGA AGAAGCGGGC GAATATCGCC 
TGTGAACTGC TGACCAATCC GCTGCTCATG CTCCTGGATG AACCCACTTC TGGACTGGAC 
AGCCACTCGG CCATTTCGCT GATGAAAGTA CTCAAGCGGT ACGCCCAGCT GGAGCAGAAG 
ACCATCGTGA TCAGCGTGCA CCAGCCTTCG TCCCAGATGT TCCACATGTT CGACAAGCTG 
CTTCTGCTCC ACCAAGGACG CACTGCGTAC TTTGGGGACG TGCAAAACAT CTACCGCCAC 
TTCGAGGACA TCGGGGTCAC CATCAAGCCG CACTACAATC CCGCCGATTT TGTGTTGGAG 
CAACTGAAAT CGCATCCGGA TATCCGCGAG AAACTCTTCA TAGCCGCCAA GGAATCGCAC 
GGCAACTATC TGAACCGCAA CTGCATCACG AGTAGCCACC ATAACCAGGT CAGCGTTTCG 
GGTGCGAAGG GCAAGAAGCA AGCGGATAGC ATCCTGATCG ACGACATAAT AAATAACTAT 
TACAATCAAC GCTCCAACCG GCACCATCAC CAGTACGAGA ACCTGCACCA CACGAGCAAC 
GGCTGCCGGG TGGAGGAGGA CGAGGAGGCG GCCCAGCACC TGGTGTGGTG TGCCGCGGAC 
TCGCAGTCCA ACTTCAGTTC CTGCGCCTCC AGCGATTGTC ATTCCTATAG TGCTGGCAGT 
GGGCCAGGAC ATTCGGCGGA TGACGACTGG CTGTCCTATC CCACCAGCTT CCACACCCAG 
TTCTGCGTCC TCTCAAGTCG CAATTTCCGG GAGGCTAAGC CGCGCATGCT GTCCAAACTC 
AATTGGTTCC AAACTATCGG ACTGGCTCTG ATGGCCGGTG CCATTTGGTT CCAGCTGCCC 
AGAACCGAGG AGTTCCTGCA CGACCTCCAG GGATGGATGT TCTTCTCGCA GACCTACTGG 
ATGCTCTTCG CCCTCTTCGG GGCCCTTAAT TCATTCCCCT CGGAGCGCGA GGTGGTGAGC 
AAGGAACGTC GCTCCGGGGC CTACCGACTA TCCGCCTATT ATTTGGCCAA AATGTGTGCC 
GAGCTGCCCT TGGTGATCAC TTTGCCCACG GTCTACCTCA TGATCAGCTA CCCCATGCTG 
GGCTGCACCA GCTTGAAGCT ATTCTTCCTG ATGCTCATCT TTCTGCTGAT CAACACGATC 
GTGGCCCAGA GCGTGGGCTT CTTCATCGGA GCCTGCTGCA TGGACATGAA CGTGAGCATC 
ACCCTGAGTG CCCTGTACAC GCTGGCCACC CAGCTGTTCG GCGGATATCT GTCTTCGCGC 
ATTCCGGAGG GGCTCAGCTG GATCCGGTAC ACCTCCATGA TCCACTACGC CTACCAGAAC 
ATGCAGATTC TCGAGTTCCG CGAGGGGGCG GCTATTGGTT GTGGCTCGCC CAGCAGTTAC 
GAGATTTGCA AACAGCAATC AACCGAATTC ATTCCATACG AGGAAATACT CAAAGCCCAA 
AACTCGACAT CACCGCTCTG GCTGAACACG CTGATCCTGA TGATGTTCTT CCTGGTATTC 
CGGTGCCTGG GATACGCGGT GTTGCGCTTC CTGCGATGCC CCAAAAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524648.3
CDS	562..3615
Translation	MIISKDLFIFGEQIFATIIPEGGAAALATQQQHQQQHQQQHQQSHQQPHQQSQQQQQQQQPAIINSTTTTATSLVINSTAGAAEQVVEESLKVAAAAAAATTTTTAAAATTLPAKQETVAVAAACVAPRKLAKIQKIPKEPEELKAKKKAKCEAAAAATHYEKMPMNLMIVESVEAVDMGSSDNDNETTEQVPLVSASRRNLAKIVADCRLQIEVEPATSAVAAAAAAAASVASPPATSDATAATSVASSDSSASSSASSTSSSSSSSTSSEFTHTSQTTIPSTDNLTSSNGLANIGKSILVDAKSNSPASLSYHFEKSAAANQQKQHHNINICNSHHPHHHSQNQPQHSLQLTFQNLNVLHNERQILSDVSGFVSPCEVLAIMGPSGSGKTTLLDCLSGQRHIDSGSVFLNREPLTKKWRRRIGYVLQEEIFFPQLTLRETVVYTALLRLPESMPRAEKMRQVDHILEALELGCCQQTKFGDYLNRGLSGGEKKRANIACELLTNPLLMLLDEPTSGLDSHSAISLMKVLKRYAQLEQKTIVISVHQPSSQMFHMFDKLLLLHQGRTAYFGDVQNIYRHFEDIGVTIKPHYNPADFVLEQLKSHPDIREKLFIAAKESHGNYLNRNCITSSHHNQVSVSGAKGKKQADSILIDDIINNYYNQRSNRHHHQYENLHHTSNGCRVEEDEEAAQHLVWCAADSQSNFSSCASSDCHSYSAGSGPGHSADDDWLSYPTSFHTQFCVLSSRNFREAKPRMLSKLNWFQTIGLALMAGAIWFQLPRTEEFLHDLQGWMFFSQTYWMLFALFGALNSFPSEREVVSKERRSGAYRLSAYYLAKMCAELPLVITLPTVYLMISYPMLGCTSLKLFFLMLIFLLINTIVAQSVGFFIGACCMDMNVSITLSALYTLATQLFGGYLSSRIPEGLSWIRYTSMIHYAYQNMQILEFREGAAIGCGSPSSYEICKQQSTEFIPYEEILKAQNSTSPLWLNTLILMMFFLVFRCLGYAVLRFLRCPKKT

Target ORF information:

RefSeq Version	NM_079909.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster early gene at 23, transcript variant D (E23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079909.3

ORF Insert Sequence:

ATGATTATTT CCAAGGATCT TTTCATATTT GGCGAACAAA TTTTTGCAAC AATCATACCA 
GAAGGTGGAG CCGCGGCGCT GGCAACCCAG CAGCAACATC AGCAGCAGCA TCAGCAGCAG 
CATCAGCAGT CGCATCAGCA GCCGCATCAG CAGTCGCAGC AGCAGCAGCA GCAACAACAG 
CCGGCAATTA TAAATTCAAC AACGACGACG GCCACTTCGT TGGTAATAAA CTCGACAGCA 
GGAGCAGCTG AACAAGTTGT AGAGGAGTCA TTAAAAGTAG CAGCAGCAGC AGCAGCAGCA 
ACAACAACAA CAACAGCCGC AGCAGCAACA ACACTACCAG CTAAACAGGA AACAGTTGCA 
GTTGCTGCCG CCTGTGTTGC TCCTCGCAAA TTGGCAAAAA TTCAGAAAAT TCCAAAGGAA 
CCCGAGGAGC TCAAAGCCAA GAAAAAGGCA AAGTGTGAGG CAGCAGCAGC GGCGACTCAT 
TACGAAAAAA TGCCAATGAA TTTGATGATC GTGGAAAGTG TTGAGGCAGT CGATATGGGA 
TCATCGGACA ATGACAACGA GACAACGGAG CAGGTACCGT TGGTGAGTGC CAGCCGTCGC 
AATCTGGCCA AAATAGTGGC CGACTGCCGC CTCCAGATCG AAGTGGAGCC GGCAACATCG 
GCAGTGGCCG CAGCAGCAGC AGCAGCAGCA AGCGTTGCAT CCCCGCCTGC AACATCGGAT 
GCGACAGCAG CGACATCGGT GGCCAGCAGC GACTCCTCTG CCTCATCATC AGCATCTTCG 
ACGTCGTCGT CATCTTCCAG CTCAACGTCC TCAGAATTTA CGCACACATC ACAGACAACG 
ATACCAAGTA CGGATAATCT AACGTCATCC AATGGTCTGG CCAATATAGG CAAAAGCATT 
TTAGTTGATG CCAAAAGTAA CTCCCCAGCT TCCTTGTCTT ACCACTTTGA GAAGTCTGCG 
GCGGCGAACC AACAGAAGCA GCATCACAAC ATAAACATCT GCAACAGCCA CCACCCACAT 
CACCACTCCC AGAATCAGCC CCAGCACAGT TTGCAGCTGA CCTTCCAGAA CCTGAACGTT 
TTGCACAACG AACGCCAAAT ACTTTCCGAT GTGAGTGGAT TCGTCAGTCC CTGCGAGGTC 
CTGGCCATCA TGGGTCCTTC GGGCAGCGGG AAGACCACTC TGCTGGACTG CCTCAGTGGT 
CAGCGGCACA TTGACAGCGG CAGTGTGTTC CTCAACCGTG AGCCCCTGAC CAAAAAGTGG 
CGTCGAAGGA TCGGCTATGT CCTCCAGGAG GAGATCTTCT TCCCGCAATT GACGCTCCGC 
GAAACGGTGG TCTATACTGC GCTGCTGCGA CTGCCGGAAT CAATGCCGCG GGCGGAGAAG 
ATGCGCCAGG TGGACCACAT ACTGGAGGCT CTGGAATTGG GTTGCTGTCA GCAGACCAAG 
TTCGGGGACT ACCTCAACCG TGGACTCTCC GGTGGCGAGA AGAAGCGGGC GAATATCGCC 
TGTGAACTGC TGACCAATCC GCTGCTCATG CTCCTGGATG AACCCACTTC TGGACTGGAC 
AGCCACTCGG CCATTTCGCT GATGAAAGTA CTCAAGCGGT ACGCCCAGCT GGAGCAGAAG 
ACCATCGTGA TCAGCGTGCA CCAGCCTTCG TCCCAGATGT TCCACATGTT CGACAAGCTG 
CTTCTGCTCC ACCAAGGACG CACTGCGTAC TTTGGGGACG TGCAAAACAT CTACCGCCAC 
TTCGAGGACA TCGGGGTCAC CATCAAGCCG CACTACAATC CCGCCGATTT TGTGTTGGAG 
CAACTGAAAT CGCATCCGGA TATCCGCGAG AAACTCTTCA TAGCCGCCAA GGAATCGCAC 
GGCAACTATC TGAACCGCAA CTGCATCACG AGTAGCCACC ATAACCAGGT CAGCGTTTCG 
GGTGCGAAGG GCAAGAAGCA AGCGGATAGC ATCCTGATCG ACGACATAAT AAATAACTAT 
TACAATCAAC GCTCCAACCG GCACCATCAC CAGTACGAGA ACCTGCACCA CACGAGCAAC 
GGCTGCCGGG TGGAGGAGGA CGAGGAGGCG GCCCAGCACC TGGTGTGGTG TGCCGCGGAC 
TCGCAGTCCA ACTTCAGTTC CTGCGCCTCC AGCGATTGTC ATTCCTATAG TGCTGGCAGT 
GGGCCAGGAC ATTCGGCGGA TGACGACTGG CTGTCCTATC CCACCAGCTT CCACACCCAG 
TTCTGCGTCC TCTCAAGTCG CAATTTCCGG GAGGCTAAGC CGCGCATGCT GTCCAAACTC 
AATTGGTTCC AAACTATCGG ACTGGCTCTG ATGGCCGGTG CCATTTGGTT CCAGCTGCCC 
AGAACCGAGG AGTTCCTGCA CGACCTCCAG GGATGGATGT TCTTCTCGCA GACCTACTGG 
ATGCTCTTCG CCCTCTTCGG GGCCCTTAAT TCATTCCCCT CGGAGCGCGA GGTGGTGAGC 
AAGGAACGTC GCTCCGGGGC CTACCGACTA TCCGCCTATT ATTTGGCCAA AATGTGTGCC 
GAGCTGCCCT TGGTGATCAC TTTGCCCACG GTCTACCTCA TGATCAGCTA CCCCATGCTG 
GGCTGCACCA GCTTGAAGCT ATTCTTCCTG ATGCTCATCT TTCTGCTGAT CAACACGATC 
GTGGCCCAGA GCGTGGGCTT CTTCATCGGA GCCTGCTGCA TGGACATGAA CGTGAGCATC 
ACCCTGAGTG CCCTGTACAC GCTGGCCACC CAGCTGTTCG GCGGATATCT GTCTTCGCGC 
ATTCCGGAGG GGCTCAGCTG GATCCGGTAC ACCTCCATGA TCCACTACGC CTACCAGAAC 
ATGCAGATTC TCGAGTTCCG CGAGGGGGCG GCTATTGGTT GTGGCTCGCC CAGCAGTTAC 
GAGATTTGCA AACAGCAATC AACCGAATTC ATTCCATACG AGGAAATACT CAAAGCCCAA 
AACTCGACAT CACCGCTCTG GCTGAACACG CTGATCCTGA TGATGTTCTT CCTGGTATTC 
CGGTGCCTGG GATACGCGGT GTTGCGCTTC CTGCGATGCC CCAAAAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

E23 ( NM_134924.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608768.2 Drosophila melanogaster early gene at 23, transcript variant E (E23), mRNA.

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CloneID	OFa15178
Clone ID Related Accession (Same CDS sequence)	NM_205900.2 , NM_079909.3 , NM_134924.2
Accession Version	NM_134924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	early gene at 23, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_134924.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATTT CCAAGGATCT TTTCATATTT GGCGAACAAA TTTTTGCAAC AATCATACCA 
GAAGGTGGAG CCGCGGCGCT GGCAACCCAG CAGCAACATC AGCAGCAGCA TCAGCAGCAG 
CATCAGCAGT CGCATCAGCA GCCGCATCAG CAGTCGCAGC AGCAGCAGCA GCAACAACAG 
CCGGCAATTA TAAATTCAAC AACGACGACG GCCACTTCGT TGGTAATAAA CTCGACAGCA 
GGAGCAGCTG AACAAGTTGT AGAGGAGTCA TTAAAAGTAG CAGCAGCAGC AGCAGCAGCA 
ACAACAACAA CAACAGCCGC AGCAGCAACA ACACTACCAG CTAAACAGGA AACAGTTGCA 
GTTGCTGCCG CCTGTGTTGC TCCTCGCAAA TTGGCAAAAA TTCAGAAAAT TCCAAAGGAA 
CCCGAGGAGC TCAAAGCCAA GAAAAAGGCA AAGTGTGAGG CAGCAGCAGC GGCGACTCAT 
TACGAAAAAA TGCCAATGAA TTTGATGATC GTGGAAAGTG TTGAGGCAGT CGATATGGGA 
TCATCGGACA ATGACAACGA GACAACGGAG CAGGTACCGT TGGTGAGTGC CAGCCGTCGC 
AATCTGGCCA AAATAGTGGC CGACTGCCGC CTCCAGATCG AAGTGGAGCC GGCAACATCG 
GCAGTGGCCG CAGCAGCAGC AGCAGCAGCA AGCGTTGCAT CCCCGCCTGC AACATCGGAT 
GCGACAGCAG CGACATCGGT GGCCAGCAGC GACTCCTCTG CCTCATCATC AGCATCTTCG 
ACGTCGTCGT CATCTTCCAG CTCAACGTCC TCAGAATTTA CGCACACATC ACAGACAACG 
ATACCAAGTA CGGATAATCT AACGTCATCC AATGGTCTGG CCAATATAGG CAAAAGCATT 
TTAGTTGATG CCAAAAGTAA CTCCCCAGCT TCCTTGTCTT ACCACTTTGA GAAGTCTGCG 
GCGGCGAACC AACAGAAGCA GCATCACAAC ATAAACATCT GCAACAGCCA CCACCCACAT 
CACCACTCCC AGAATCAGCC CCAGCACAGT TTGCAGCTGA CCTTCCAGAA CCTGAACGTT 
TTGCACAACG AACGCCAAAT ACTTTCCGAT GTGAGTGGAT TCGTCAGTCC CTGCGAGGTC 
CTGGCCATCA TGGGTCCTTC GGGCAGCGGG AAGACCACTC TGCTGGACTG CCTCAGTGGT 
CAGCGGCACA TTGACAGCGG CAGTGTGTTC CTCAACCGTG AGCCCCTGAC CAAAAAGTGG 
CGTCGAAGGA TCGGCTATGT CCTCCAGGAG GAGATCTTCT TCCCGCAATT GACGCTCCGC 
GAAACGGTGG TCTATACTGC GCTGCTGCGA CTGCCGGAAT CAATGCCGCG GGCGGAGAAG 
ATGCGCCAGG TGGACCACAT ACTGGAGGCT CTGGAATTGG GTTGCTGTCA GCAGACCAAG 
TTCGGGGACT ACCTCAACCG TGGACTCTCC GGTGGCGAGA AGAAGCGGGC GAATATCGCC 
TGTGAACTGC TGACCAATCC GCTGCTCATG CTCCTGGATG AACCCACTTC TGGACTGGAC 
AGCCACTCGG CCATTTCGCT GATGAAAGTA CTCAAGCGGT ACGCCCAGCT GGAGCAGAAG 
ACCATCGTGA TCAGCGTGCA CCAGCCTTCG TCCCAGATGT TCCACATGTT CGACAAGCTG 
CTTCTGCTCC ACCAAGGACG CACTGCGTAC TTTGGGGACG TGCAAAACAT CTACCGCCAC 
TTCGAGGACA TCGGGGTCAC CATCAAGCCG CACTACAATC CCGCCGATTT TGTGTTGGAG 
CAACTGAAAT CGCATCCGGA TATCCGCGAG AAACTCTTCA TAGCCGCCAA GGAATCGCAC 
GGCAACTATC TGAACCGCAA CTGCATCACG AGTAGCCACC ATAACCAGGT CAGCGTTTCG 
GGTGCGAAGG GCAAGAAGCA AGCGGATAGC ATCCTGATCG ACGACATAAT AAATAACTAT 
TACAATCAAC GCTCCAACCG GCACCATCAC CAGTACGAGA ACCTGCACCA CACGAGCAAC 
GGCTGCCGGG TGGAGGAGGA CGAGGAGGCG GCCCAGCACC TGGTGTGGTG TGCCGCGGAC 
TCGCAGTCCA ACTTCAGTTC CTGCGCCTCC AGCGATTGTC ATTCCTATAG TGCTGGCAGT 
GGGCCAGGAC ATTCGGCGGA TGACGACTGG CTGTCCTATC CCACCAGCTT CCACACCCAG 
TTCTGCGTCC TCTCAAGTCG CAATTTCCGG GAGGCTAAGC CGCGCATGCT GTCCAAACTC 
AATTGGTTCC AAACTATCGG ACTGGCTCTG ATGGCCGGTG CCATTTGGTT CCAGCTGCCC 
AGAACCGAGG AGTTCCTGCA CGACCTCCAG GGATGGATGT TCTTCTCGCA GACCTACTGG 
ATGCTCTTCG CCCTCTTCGG GGCCCTTAAT TCATTCCCCT CGGAGCGCGA GGTGGTGAGC 
AAGGAACGTC GCTCCGGGGC CTACCGACTA TCCGCCTATT ATTTGGCCAA AATGTGTGCC 
GAGCTGCCCT TGGTGATCAC TTTGCCCACG GTCTACCTCA TGATCAGCTA CCCCATGCTG 
GGCTGCACCA GCTTGAAGCT ATTCTTCCTG ATGCTCATCT TTCTGCTGAT CAACACGATC 
GTGGCCCAGA GCGTGGGCTT CTTCATCGGA GCCTGCTGCA TGGACATGAA CGTGAGCATC 
ACCCTGAGTG CCCTGTACAC GCTGGCCACC CAGCTGTTCG GCGGATATCT GTCTTCGCGC 
ATTCCGGAGG GGCTCAGCTG GATCCGGTAC ACCTCCATGA TCCACTACGC CTACCAGAAC 
ATGCAGATTC TCGAGTTCCG CGAGGGGGCG GCTATTGGTT GTGGCTCGCC CAGCAGTTAC 
GAGATTTGCA AACAGCAATC AACCGAATTC ATTCCATACG AGGAAATACT CAAAGCCCAA 
AACTCGACAT CACCGCTCTG GCTGAACACG CTGATCCTGA TGATGTTCTT CCTGGTATTC 
CGGTGCCTGG GATACGCGGT GTTGCGCTTC CTGCGATGCC CCAAAAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608768.2
CDS	1314..4367
Translation	MIISKDLFIFGEQIFATIIPEGGAAALATQQQHQQQHQQQHQQSHQQPHQQSQQQQQQQQPAIINSTTTTATSLVINSTAGAAEQVVEESLKVAAAAAAATTTTTAAAATTLPAKQETVAVAAACVAPRKLAKIQKIPKEPEELKAKKKAKCEAAAAATHYEKMPMNLMIVESVEAVDMGSSDNDNETTEQVPLVSASRRNLAKIVADCRLQIEVEPATSAVAAAAAAAASVASPPATSDATAATSVASSDSSASSSASSTSSSSSSSTSSEFTHTSQTTIPSTDNLTSSNGLANIGKSILVDAKSNSPASLSYHFEKSAAANQQKQHHNINICNSHHPHHHSQNQPQHSLQLTFQNLNVLHNERQILSDVSGFVSPCEVLAIMGPSGSGKTTLLDCLSGQRHIDSGSVFLNREPLTKKWRRRIGYVLQEEIFFPQLTLRETVVYTALLRLPESMPRAEKMRQVDHILEALELGCCQQTKFGDYLNRGLSGGEKKRANIACELLTNPLLMLLDEPTSGLDSHSAISLMKVLKRYAQLEQKTIVISVHQPSSQMFHMFDKLLLLHQGRTAYFGDVQNIYRHFEDIGVTIKPHYNPADFVLEQLKSHPDIREKLFIAAKESHGNYLNRNCITSSHHNQVSVSGAKGKKQADSILIDDIINNYYNQRSNRHHHQYENLHHTSNGCRVEEDEEAAQHLVWCAADSQSNFSSCASSDCHSYSAGSGPGHSADDDWLSYPTSFHTQFCVLSSRNFREAKPRMLSKLNWFQTIGLALMAGAIWFQLPRTEEFLHDLQGWMFFSQTYWMLFALFGALNSFPSEREVVSKERRSGAYRLSAYYLAKMCAELPLVITLPTVYLMISYPMLGCTSLKLFFLMLIFLLINTIVAQSVGFFIGACCMDMNVSITLSALYTLATQLFGGYLSSRIPEGLSWIRYTSMIHYAYQNMQILEFREGAAIGCGSPSSYEICKQQSTEFIPYEEILKAQNSTSPLWLNTLILMMFFLVFRCLGYAVLRFLRCPKKT

Target ORF information:

RefSeq Version	NM_134924.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster early gene at 23, transcript variant E (E23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134924.2

ORF Insert Sequence:

ATGATTATTT CCAAGGATCT TTTCATATTT GGCGAACAAA TTTTTGCAAC AATCATACCA 
GAAGGTGGAG CCGCGGCGCT GGCAACCCAG CAGCAACATC AGCAGCAGCA TCAGCAGCAG 
CATCAGCAGT CGCATCAGCA GCCGCATCAG CAGTCGCAGC AGCAGCAGCA GCAACAACAG 
CCGGCAATTA TAAATTCAAC AACGACGACG GCCACTTCGT TGGTAATAAA CTCGACAGCA 
GGAGCAGCTG AACAAGTTGT AGAGGAGTCA TTAAAAGTAG CAGCAGCAGC AGCAGCAGCA 
ACAACAACAA CAACAGCCGC AGCAGCAACA ACACTACCAG CTAAACAGGA AACAGTTGCA 
GTTGCTGCCG CCTGTGTTGC TCCTCGCAAA TTGGCAAAAA TTCAGAAAAT TCCAAAGGAA 
CCCGAGGAGC TCAAAGCCAA GAAAAAGGCA AAGTGTGAGG CAGCAGCAGC GGCGACTCAT 
TACGAAAAAA TGCCAATGAA TTTGATGATC GTGGAAAGTG TTGAGGCAGT CGATATGGGA 
TCATCGGACA ATGACAACGA GACAACGGAG CAGGTACCGT TGGTGAGTGC CAGCCGTCGC 
AATCTGGCCA AAATAGTGGC CGACTGCCGC CTCCAGATCG AAGTGGAGCC GGCAACATCG 
GCAGTGGCCG CAGCAGCAGC AGCAGCAGCA AGCGTTGCAT CCCCGCCTGC AACATCGGAT 
GCGACAGCAG CGACATCGGT GGCCAGCAGC GACTCCTCTG CCTCATCATC AGCATCTTCG 
ACGTCGTCGT CATCTTCCAG CTCAACGTCC TCAGAATTTA CGCACACATC ACAGACAACG 
ATACCAAGTA CGGATAATCT AACGTCATCC AATGGTCTGG CCAATATAGG CAAAAGCATT 
TTAGTTGATG CCAAAAGTAA CTCCCCAGCT TCCTTGTCTT ACCACTTTGA GAAGTCTGCG 
GCGGCGAACC AACAGAAGCA GCATCACAAC ATAAACATCT GCAACAGCCA CCACCCACAT 
CACCACTCCC AGAATCAGCC CCAGCACAGT TTGCAGCTGA CCTTCCAGAA CCTGAACGTT 
TTGCACAACG AACGCCAAAT ACTTTCCGAT GTGAGTGGAT TCGTCAGTCC CTGCGAGGTC 
CTGGCCATCA TGGGTCCTTC GGGCAGCGGG AAGACCACTC TGCTGGACTG CCTCAGTGGT 
CAGCGGCACA TTGACAGCGG CAGTGTGTTC CTCAACCGTG AGCCCCTGAC CAAAAAGTGG 
CGTCGAAGGA TCGGCTATGT CCTCCAGGAG GAGATCTTCT TCCCGCAATT GACGCTCCGC 
GAAACGGTGG TCTATACTGC GCTGCTGCGA CTGCCGGAAT CAATGCCGCG GGCGGAGAAG 
ATGCGCCAGG TGGACCACAT ACTGGAGGCT CTGGAATTGG GTTGCTGTCA GCAGACCAAG 
TTCGGGGACT ACCTCAACCG TGGACTCTCC GGTGGCGAGA AGAAGCGGGC GAATATCGCC 
TGTGAACTGC TGACCAATCC GCTGCTCATG CTCCTGGATG AACCCACTTC TGGACTGGAC 
AGCCACTCGG CCATTTCGCT GATGAAAGTA CTCAAGCGGT ACGCCCAGCT GGAGCAGAAG 
ACCATCGTGA TCAGCGTGCA CCAGCCTTCG TCCCAGATGT TCCACATGTT CGACAAGCTG 
CTTCTGCTCC ACCAAGGACG CACTGCGTAC TTTGGGGACG TGCAAAACAT CTACCGCCAC 
TTCGAGGACA TCGGGGTCAC CATCAAGCCG CACTACAATC CCGCCGATTT TGTGTTGGAG 
CAACTGAAAT CGCATCCGGA TATCCGCGAG AAACTCTTCA TAGCCGCCAA GGAATCGCAC 
GGCAACTATC TGAACCGCAA CTGCATCACG AGTAGCCACC ATAACCAGGT CAGCGTTTCG 
GGTGCGAAGG GCAAGAAGCA AGCGGATAGC ATCCTGATCG ACGACATAAT AAATAACTAT 
TACAATCAAC GCTCCAACCG GCACCATCAC CAGTACGAGA ACCTGCACCA CACGAGCAAC 
GGCTGCCGGG TGGAGGAGGA CGAGGAGGCG GCCCAGCACC TGGTGTGGTG TGCCGCGGAC 
TCGCAGTCCA ACTTCAGTTC CTGCGCCTCC AGCGATTGTC ATTCCTATAG TGCTGGCAGT 
GGGCCAGGAC ATTCGGCGGA TGACGACTGG CTGTCCTATC CCACCAGCTT CCACACCCAG 
TTCTGCGTCC TCTCAAGTCG CAATTTCCGG GAGGCTAAGC CGCGCATGCT GTCCAAACTC 
AATTGGTTCC AAACTATCGG ACTGGCTCTG ATGGCCGGTG CCATTTGGTT CCAGCTGCCC 
AGAACCGAGG AGTTCCTGCA CGACCTCCAG GGATGGATGT TCTTCTCGCA GACCTACTGG 
ATGCTCTTCG CCCTCTTCGG GGCCCTTAAT TCATTCCCCT CGGAGCGCGA GGTGGTGAGC 
AAGGAACGTC GCTCCGGGGC CTACCGACTA TCCGCCTATT ATTTGGCCAA AATGTGTGCC 
GAGCTGCCCT TGGTGATCAC TTTGCCCACG GTCTACCTCA TGATCAGCTA CCCCATGCTG 
GGCTGCACCA GCTTGAAGCT ATTCTTCCTG ATGCTCATCT TTCTGCTGAT CAACACGATC 
GTGGCCCAGA GCGTGGGCTT CTTCATCGGA GCCTGCTGCA TGGACATGAA CGTGAGCATC 
ACCCTGAGTG CCCTGTACAC GCTGGCCACC CAGCTGTTCG GCGGATATCT GTCTTCGCGC 
ATTCCGGAGG GGCTCAGCTG GATCCGGTAC ACCTCCATGA TCCACTACGC CTACCAGAAC 
ATGCAGATTC TCGAGTTCCG CGAGGGGGCG GCTATTGGTT GTGGCTCGCC CAGCAGTTAC 
GAGATTTGCA AACAGCAATC AACCGAATTC ATTCCATACG AGGAAATACT CAAAGCCCAA 
AACTCGACAT CACCGCTCTG GCTGAACACG CTGATCCTGA TGATGTTCTT CCTGGTATTC 
CGGTGCCTGG GATACGCGGT GTTGCGCTTC CTGCGATGCC CCAAAAAGAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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E23 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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