Gyf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gyf
Entrez Gene ID	43842
Full Name	Gigyf
Synonyms	CG11148,Dmel\CG11148,Q7KQM6
General protein information	Preferred Names Gigyf Names CG11148 gene product from transcript CG11148-RH CG11148-PD CG11148-PF CG11148-PG CG11148-PH Gyf-PD Gyf-PF Gyf-PG Gyf-PH gigyf
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102E5-102E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_143693.4	NP_651950.3	gigyf, isoform D
NM_205878.3	NP_995600.2	gigyf, isoform F
NM_001169357.4	NP_001162828.1	gigyf, isoform H
NM_001169356.2	NP_001162827.1	gigyf, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CG11148 is known as GYF; its product is orthologous to GIGYF1 (GRB10-interacting GYF protein 1) and GIGYF2 in mammals, and it is a new autophagy regulator
Title: Drosophila Gyf/GRB10 interacting GYF protein is an autophagy regulator that controls neuron and muscle homeostasis.

The following Gyf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gyf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00204	NM_143693.4 Latest version!	Drosophila melanogaster gigyf, transcript variant D (Gyf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00204	NM_205878.3 Latest version!	Drosophila melanogaster gigyf, transcript variant F (Gyf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00319	NM_001169357.4 Latest version!	Drosophila melanogaster gigyf, transcript variant H (Gyf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa00319	NM_001169356.2 Latest version!	Drosophila melanogaster gigyf, transcript variant G (Gyf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gyf ( NM_143693.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651950.3 Drosophila melanogaster gigyf, transcript variant D (Gyf), mRNA.

Price & Availability↑

CloneID	OFa00204
Clone ID Related Accession (Same CDS sequence)	NM_143693.4 , NM_205878.3
Accession Version	NM_143693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gigyf, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_143693.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACCGCT CGCCCAGTGG ATTTGGTAGT 
GCCTCCCGAG GCTCTGGACG TGGTGGAACC GTTGACCGGG GACGGATGCG CGGAAAATCG 
GCCTATCATC CGATATACCA ACGCCCAAGC GGTCTATATG ATGAAAGCTT ATCGGTTATA 
TCAAAGGCCG AACGCACATG GAGCGACCGC AACGGAACTG GAGATTCTGC GGCAACCACC 
ACCTCCACTA GTGGCTCTGG TGCTCTAGAT TGGAATGGAA CTCCAAGCTC AAGTCCTCGA 
AAAGATTATT CTAATAATCA TCGCAACTTG GAAAACTGGC GACGAACACG TAACGAAGAT 
GGATCCGGTG ATGGTCCATC CTCCAGCGGT TCCATGAGCG GACCCGATAT TGCTGGTTGG 
CGGAGCGGTG TCGTCGGTGG AAGTACAAAC GCAGGTTTTG GTACCAATAG CCATCGGTGG 
GGAAGATCTA CAAGCTGGCG TGATGAAGAT CCTAGCGTCG ACAATGCAGC TAGTTTACAA 
CGCTCAATTT CAACAGTTGC GACTTTAAGT ACAGATCGGA CTGGAAACAA TAAAGGATCT 
GGAATCGGAG CTGCAGAGGG AGTAGGGAGC ATATCGCACC CACGGTTGTC GAGTTCTAAA 
ATATCTCAAT TATGGACTGT TAATAATGCA GCCGGTGTTG ATGCCGACGA AAATCTACCC 
GAATGGGCAA TTGAAAATCC ATCGAAATTG GGTGGCAGTT TTGATGCTAG TGGAGCCTTT 
CATGGAGATA CTGATCTAAA ACCTATCAAA AGTTCGCATA ATACTTTAAA AACCAAAAGC 
TTAGACTCCT ATGACGATGT AAAAAGACCG AAAAGTAAAG ACCTGTCTGA TCCAGATAGT 
GGAAACGATA TAACTCCAGA AACCTCATTA ACGAAAGATA CCAATACAAC AGCTGTGCAA 
GAAGAAGTTG AAAGCAGTTT ATCTCCAATA AGCTCTACAA CGACAAAGGA AGTGATCCAT 
GGAGATATTT CAGATCGGAT TAAAGAGGTC GCCGATGAAG TAGAAAAACT TATAATGGAT 
GATGATCATA AAATCTCTTC GAATCAAAGC CAACATCAAA ATCATGACGG GTTTACAGCT 
GCACTTCCAA GAATGGCAGA CATTGAGATA AACGTAAAAC CGAGTGTTAC AGCAGTGCAT 
CGGCAAGCAC CTTCCACCAT GCCTATACAA ATTACCCCTA CAATTACAGA TGTCGCTCCA 
CCATCCCATG CAGTTGTGTC GTTTTCGGAT CATGAGACAA TGCAACACCA TAACATGCAC 
CACCTGCCAC AATTTCCAAT GATTCCTACA CCGCATATCA TCACTCCAAA TCTAAACGAA 
TTGTGGTTTT ACCGGGATCC GCAGGCAAAT GTTCAGGGGC CATTTAGTGC CGTTGAGATG 
ACGGAATGGT ATCGCGCTGG CTACTTTAAT GAGAACCTGT TTGTACGCCG GTACTCTGAT 
AATAGGTTTA GACCGCTGGG AGAGCTTATA AAATTTTGTC ATGGTAACAT GCCATTTACG 
CACAGTCACC TACTTCCATC TCCGATAGAA CTAGAGAACA TTCCTGTTGG TCAAATACCA 
GCCCCTCTAA CGGCGTCACT TTCAATTACA CCCCATAAGC CATCACCGAT TCCTATCGCA 
CTTTCTGTTG TTGAACAGCA GTTGCAGCAA CAAAGAGATG AGCAACTAAA GGCAAATGTT 
ACAGCAACAG CTGAAGCTCT ACGTGCTGCA ATAAAAGGAA GTTTTGGCGG AAATAGCATT 
GGTAATACGT CTCATTTGCT TACAATGCGG TTTCAAATGC TACAGGATCA GTACATACAG 
CACCAGGAAT ACCAAATACT AGCTGAGCTA TCCAAAAATG AATGCTTTCA ACGTCTTTCG 
GCTGTCGAGC AAGAAACAGT TGTTCGTCGG AAAGTTCAAT TGCTGGGTCT TCCTGAGTAT 
TTGATCAGCT TAAACGGATT AAGCAATTCT TTGTCCGTAC TGAACCCCGT TGCCGGTAGA 
CAGTTGTACA GGGCAGTGGT TGAGCACGCC AAGAAGGATC AACAACATAT TTTTGCAAAC 
ACCGAGCAGC AACGTTCAGT GGGAAATTTA CTGGATGCTA ATAATTTCAT TCTAAATGCC 
CAAATAATGA TTCAGCAATC GCAGCAAGAG GTAGGTCCCT TGGTATCATC CGTGGATTGT 
ATTATGCAAG GTGGAACTGC AGCTGACCTA AATAAGCCGA ATGAAATACC GAGGAATGAG 
TTAGACTTAA TTAATGAATA TAACTTACGG ATGCTGTTGC GTGGCCAACC AACAAGTACT 
CAACAGCAAC CACCTGCGCT GACGAACTCA GCTACCGAAA ATCTCCCTGG AGTTGATTTT 
TTAACTGAAA CACAATTGTT AGAGCGGCAA AACTTGATGA TTCCAATCTG GTTACCCCCT 
AATAATAATA AGCAACAAGA AACCGACCAA CAGTGGGCTG AAATGTCTAA TGCGGATGCA 
TCATTATGGG AAACGGCCAA CTTAAATGAA GAGCGAAATG AAGATCAGCA ACTATTAATA 
CAAAAGTCTT CTGAAGCGTG CTTCGCAGAT ACAGAAAAAG ATGTCAAAAT TGCCCAATTA 
TTTCAAGTTC AATCAGGAAA TGTTGTTAAC CACACCGCAT TAGAGGAATT AGATCAGTCC 
CCTCAAAATT TAAAAGGTTC ACATAATCAA AAAATAGTCA AATCCCTTGT TTCCGATATT 
CAACAGAATC ATAATGAGGA ACTAAATTCA CATCAACACC AGGTAAAGCA GGCTAACAAG 
CAGAATCTGA ATACAAAACA GAATGCGGCA CAGTCAGCCC TAAAGCCAAT TAATAACGAA 
AATGATCGCA AACGAGAACA GACAGAAGAA AAAAAAAGGC AAAGAGAGGA ACGCAAGCGC 
CAGCAATTGG AAGATGATAA ACGTCGGGCG TTGAATGAAT CTGAAGAACA GACCCGCCAA 
ATTCAAGAGG AAAAGGAAAG GCAACAGCAA ATACAAGCCC AACGTCGAAA GGCATTGTTG 
GGCAATGTCC ATTCTCTATC AGTTCAAAAT GGGATGTCAG GAACATTAGC GTCTGCACAA 
AGCAAGAAGA ACGACGATGC CAAAACAGCA GAACCCCAAG TATCCTCGCG CTTGCCGTCT 
ACATCCGTAG CGCCTTGGTC TTTTCAGTTG CAAAATTCTA TGAGATCGGC GCCTGGTCTG 
GCAGAAATAC AAAAGGCAGA ACGTCGAGAG CGTCGCGCAG ACCAACAGCG ACATCAAGAG 
CTATTAGACA AGCAATTGCG TGCCAATGCC GCAGCTGCGG CTGAAGCCAA CGATGCTTTG 
CTTAAATGGC AGTCAACACC AGCGTCGGCG CCCGTAATGA GTCTCGCCGA AATTCAAGCA 
GAAGAGGCAA GGCGGTTGGC CAATGACCTT GTGGATCGGC AGCGTCGACG AGAACTGGAA 
CATCACCAAC AAGCTCCTTT GTCATCAGCG GTTTTGGTTA CGAGTGCAAC TTCCAACATC 
TGGGGTAACG CCAATAAAGC ATGGAGTTCG TCCGCTGCTC AATCACTTTC GTTAAAAACA 
AGTTCTGGAA CTGGTCTGTG GGACGAACCG AATCCATTAG GTTCTAATGG ATCTGGAACA 
AGCGGTACCT CATCCGTAAC TGCGGCGGCA GTCTTGGCAG GAGGATTAAA CTCAGCTAAT 
AAATCCACTC TGCAAGCTCA AAATAAGTCG TCGGCTTTAT TTGCATCGCC TCGAAATTTG 
CGCAAGAGTC AAACATTGCC AGCCCTTAGT AACCCAGAAA AGTCAAATAA AAATGGACCA 
GGTCAACGGC CAGAAAAACA AAAGTTGGCT CAAACTCGTT CAAAGGGTGC CGCTGTCTCA 
ATTGAAGAGA AGGATAGAGA AAGAAAATTG AATGCAAAAA GCCAGCAAAG CAGCACCGAC 
CAAGCCATTA GCAAGGTTAA TGAGTATGAA AACGAGTTCA CTAGCTGGTG CATAAAGAGC 
CTAGATAATA TGTCCGCCAA AGTTGATGTA CCCACGTTCG TTGCATTCTT GCAGGACTTG 
GAAGCGCCAT ATGAGGTAAA GGACTATGTC CGAATATACC TTGGCGATGG AAAAGATTCT 
TTGGATTTCG CAAAGCAGTT TTTGGAGCGA CGTAGCAAAT ACAAAAGCTT GCAACGTGCC 
CAAAAGGCAC ACAATGACGA TATGTGCAAA CCAGCTCCTG CCATTACGCC ATCTGCGAAT 
GACTATGCCG ACAGCAAGAA CAAACAAAAA AAGATTAAAA AGAATAAGAT GACTAAGATG 
GACGCCCGCA TTCTTGGGTT TTCAGTAACT GCTGCCGAGG GTCGCATAAA TGTTGGCATT 
CGAGACTACG TCGAAGGACC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651950.3
CDS	79..4782
Translation	MTDSMKFGPEWLRNMSAEPSGSPSTYNVGNAAQNNSIGGHNLGNNTAASASRNLFPEYRYGREEMLSLFDRNCLLPQILPSFKKLFVEKVQCPLALTPSSEEDINQNSLGNNRSPSGFGSASRGSGRGGTVDRGRMRGKSAYHPIYQRPSGLYDESLSVISKAERTWSDRNGTGDSAATTTSTSGSGALDWNGTPSSSPRKDYSNNHRNLENWRRTRNEDGSGDGPSSSGSMSGPDIAGWRSGVVGGSTNAGFGTNSHRWGRSTSWRDEDPSVDNAASLQRSISTVATLSTDRTGNNKGSGIGAAEGVGSISHPRLSSSKISQLWTVNNAAGVDADENLPEWAIENPSKLGGSFDASGAFHGDTDLKPIKSSHNTLKTKSLDSYDDVKRPKSKDLSDPDSGNDITPETSLTKDTNTTAVQEEVESSLSPISSTTTKEVIHGDISDRIKEVADEVEKLIMDDDHKISSNQSQHQNHDGFTAALPRMADIEINVKPSVTAVHRQAPSTMPIQITPTITDVAPPSHAVVSFSDHETMQHHNMHHLPQFPMIPTPHIITPNLNELWFYRDPQANVQGPFSAVEMTEWYRAGYFNENLFVRRYSDNRFRPLGELIKFCHGNMPFTHSHLLPSPIELENIPVGQIPAPLTASLSITPHKPSPIPIALSVVEQQLQQQRDEQLKANVTATAEALRAAIKGSFGGNSIGNTSHLLTMRFQMLQDQYIQHQEYQILAELSKNECFQRLSAVEQETVVRRKVQLLGLPEYLISLNGLSNSLSVLNPVAGRQLYRAVVEHAKKDQQHIFANTEQQRSVGNLLDANNFILNAQIMIQQSQQEVGPLVSSVDCIMQGGTAADLNKPNEIPRNELDLINEYNLRMLLRGQPTSTQQQPPALTNSATENLPGVDFLTETQLLERQNLMIPIWLPPNNNKQQETDQQWAEMSNADASLWETANLNEERNEDQQLLIQKSSEACFADTEKDVKIAQLFQVQSGNVVNHTALEELDQSPQNLKGSHNQKIVKSLVSDIQQNHNEELNSHQHQVKQANKQNLNTKQNAAQSALKPINNENDRKREQTEEKKRQREERKRQQLEDDKRRALNESEEQTRQIQEEKERQQQIQAQRRKALLGNVHSLSVQNGMSGTLASAQSKKNDDAKTAEPQVSSRLPSTSVAPWSFQLQNSMRSAPGLAEIQKAERRERRADQQRHQELLDKQLRANAAAAAEANDALLKWQSTPASAPVMSLAEIQAEEARRLANDLVDRQRRRELEHHQQAPLSSAVLVTSATSNIWGNANKAWSSSAAQSLSLKTSSGTGLWDEPNPLGSNGSGTSGTSSVTAAAVLAGGLNSANKSTLQAQNKSSALFASPRNLRKSQTLPALSNPEKSNKNGPGQRPEKQKLAQTRSKGAAVSIEEKDRERKLNAKSQQSSTDQAISKVNEYENEFTSWCIKSLDNMSAKVDVPTFVAFLQDLEAPYEVKDYVRIYLGDGKDSLDFAKQFLERRSKYKSLQRAQKAHNDDMCKPAPAITPSANDYADSKNKQKKIKKNKMTKMDARILGFSVTAAEGRINVGIRDYVEGP

Target ORF information:

RefSeq Version	NM_143693.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gigyf, transcript variant D (Gyf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143693.4

ORF Insert Sequence:

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACCGCT CGCCCAGTGG ATTTGGTAGT 
GCCTCCCGAG GCTCTGGACG TGGTGGAACC GTTGACCGGG GACGGATGCG CGGAAAATCG 
GCCTATCATC CGATATACCA ACGCCCAAGC GGTCTATATG ATGAAAGCTT ATCGGTTATA 
TCAAAGGCCG AACGCACATG GAGCGACCGC AACGGAACTG GAGATTCTGC GGCAACCACC 
ACCTCCACTA GTGGCTCTGG TGCTCTAGAT TGGAATGGAA CTCCAAGCTC AAGTCCTCGA 
AAAGATTATT CTAATAATCA TCGCAACTTG GAAAACTGGC GACGAACACG TAACGAAGAT 
GGATCCGGTG ATGGTCCATC CTCCAGCGGT TCCATGAGCG GACCCGATAT TGCTGGTTGG 
CGGAGCGGTG TCGTCGGTGG AAGTACAAAC GCAGGTTTTG GTACCAATAG CCATCGGTGG 
GGAAGATCTA CAAGCTGGCG TGATGAAGAT CCTAGCGTCG ACAATGCAGC TAGTTTACAA 
CGCTCAATTT CAACAGTTGC GACTTTAAGT ACAGATCGGA CTGGAAACAA TAAAGGATCT 
GGAATCGGAG CTGCAGAGGG AGTAGGGAGC ATATCGCACC CACGGTTGTC GAGTTCTAAA 
ATATCTCAAT TATGGACTGT TAATAATGCA GCCGGTGTTG ATGCCGACGA AAATCTACCC 
GAATGGGCAA TTGAAAATCC ATCGAAATTG GGTGGCAGTT TTGATGCTAG TGGAGCCTTT 
CATGGAGATA CTGATCTAAA ACCTATCAAA AGTTCGCATA ATACTTTAAA AACCAAAAGC 
TTAGACTCCT ATGACGATGT AAAAAGACCG AAAAGTAAAG ACCTGTCTGA TCCAGATAGT 
GGAAACGATA TAACTCCAGA AACCTCATTA ACGAAAGATA CCAATACAAC AGCTGTGCAA 
GAAGAAGTTG AAAGCAGTTT ATCTCCAATA AGCTCTACAA CGACAAAGGA AGTGATCCAT 
GGAGATATTT CAGATCGGAT TAAAGAGGTC GCCGATGAAG TAGAAAAACT TATAATGGAT 
GATGATCATA AAATCTCTTC GAATCAAAGC CAACATCAAA ATCATGACGG GTTTACAGCT 
GCACTTCCAA GAATGGCAGA CATTGAGATA AACGTAAAAC CGAGTGTTAC AGCAGTGCAT 
CGGCAAGCAC CTTCCACCAT GCCTATACAA ATTACCCCTA CAATTACAGA TGTCGCTCCA 
CCATCCCATG CAGTTGTGTC GTTTTCGGAT CATGAGACAA TGCAACACCA TAACATGCAC 
CACCTGCCAC AATTTCCAAT GATTCCTACA CCGCATATCA TCACTCCAAA TCTAAACGAA 
TTGTGGTTTT ACCGGGATCC GCAGGCAAAT GTTCAGGGGC CATTTAGTGC CGTTGAGATG 
ACGGAATGGT ATCGCGCTGG CTACTTTAAT GAGAACCTGT TTGTACGCCG GTACTCTGAT 
AATAGGTTTA GACCGCTGGG AGAGCTTATA AAATTTTGTC ATGGTAACAT GCCATTTACG 
CACAGTCACC TACTTCCATC TCCGATAGAA CTAGAGAACA TTCCTGTTGG TCAAATACCA 
GCCCCTCTAA CGGCGTCACT TTCAATTACA CCCCATAAGC CATCACCGAT TCCTATCGCA 
CTTTCTGTTG TTGAACAGCA GTTGCAGCAA CAAAGAGATG AGCAACTAAA GGCAAATGTT 
ACAGCAACAG CTGAAGCTCT ACGTGCTGCA ATAAAAGGAA GTTTTGGCGG AAATAGCATT 
GGTAATACGT CTCATTTGCT TACAATGCGG TTTCAAATGC TACAGGATCA GTACATACAG 
CACCAGGAAT ACCAAATACT AGCTGAGCTA TCCAAAAATG AATGCTTTCA ACGTCTTTCG 
GCTGTCGAGC AAGAAACAGT TGTTCGTCGG AAAGTTCAAT TGCTGGGTCT TCCTGAGTAT 
TTGATCAGCT TAAACGGATT AAGCAATTCT TTGTCCGTAC TGAACCCCGT TGCCGGTAGA 
CAGTTGTACA GGGCAGTGGT TGAGCACGCC AAGAAGGATC AACAACATAT TTTTGCAAAC 
ACCGAGCAGC AACGTTCAGT GGGAAATTTA CTGGATGCTA ATAATTTCAT TCTAAATGCC 
CAAATAATGA TTCAGCAATC GCAGCAAGAG GTAGGTCCCT TGGTATCATC CGTGGATTGT 
ATTATGCAAG GTGGAACTGC AGCTGACCTA AATAAGCCGA ATGAAATACC GAGGAATGAG 
TTAGACTTAA TTAATGAATA TAACTTACGG ATGCTGTTGC GTGGCCAACC AACAAGTACT 
CAACAGCAAC CACCTGCGCT GACGAACTCA GCTACCGAAA ATCTCCCTGG AGTTGATTTT 
TTAACTGAAA CACAATTGTT AGAGCGGCAA AACTTGATGA TTCCAATCTG GTTACCCCCT 
AATAATAATA AGCAACAAGA AACCGACCAA CAGTGGGCTG AAATGTCTAA TGCGGATGCA 
TCATTATGGG AAACGGCCAA CTTAAATGAA GAGCGAAATG AAGATCAGCA ACTATTAATA 
CAAAAGTCTT CTGAAGCGTG CTTCGCAGAT ACAGAAAAAG ATGTCAAAAT TGCCCAATTA 
TTTCAAGTTC AATCAGGAAA TGTTGTTAAC CACACCGCAT TAGAGGAATT AGATCAGTCC 
CCTCAAAATT TAAAAGGTTC ACATAATCAA AAAATAGTCA AATCCCTTGT TTCCGATATT 
CAACAGAATC ATAATGAGGA ACTAAATTCA CATCAACACC AGGTAAAGCA GGCTAACAAG 
CAGAATCTGA ATACAAAACA GAATGCGGCA CAGTCAGCCC TAAAGCCAAT TAATAACGAA 
AATGATCGCA AACGAGAACA GACAGAAGAA AAAAAAAGGC AAAGAGAGGA ACGCAAGCGC 
CAGCAATTGG AAGATGATAA ACGTCGGGCG TTGAATGAAT CTGAAGAACA GACCCGCCAA 
ATTCAAGAGG AAAAGGAAAG GCAACAGCAA ATACAAGCCC AACGTCGAAA GGCATTGTTG 
GGCAATGTCC ATTCTCTATC AGTTCAAAAT GGGATGTCAG GAACATTAGC GTCTGCACAA 
AGCAAGAAGA ACGACGATGC CAAAACAGCA GAACCCCAAG TATCCTCGCG CTTGCCGTCT 
ACATCCGTAG CGCCTTGGTC TTTTCAGTTG CAAAATTCTA TGAGATCGGC GCCTGGTCTG 
GCAGAAATAC AAAAGGCAGA ACGTCGAGAG CGTCGCGCAG ACCAACAGCG ACATCAAGAG 
CTATTAGACA AGCAATTGCG TGCCAATGCC GCAGCTGCGG CTGAAGCCAA CGATGCTTTG 
CTTAAATGGC AGTCAACACC AGCGTCGGCG CCCGTAATGA GTCTCGCCGA AATTCAAGCA 
GAAGAGGCAA GGCGGTTGGC CAATGACCTT GTGGATCGGC AGCGTCGACG AGAACTGGAA 
CATCACCAAC AAGCTCCTTT GTCATCAGCG GTTTTGGTTA CGAGTGCAAC TTCCAACATC 
TGGGGTAACG CCAATAAAGC ATGGAGTTCG TCCGCTGCTC AATCACTTTC GTTAAAAACA 
AGTTCTGGAA CTGGTCTGTG GGACGAACCG AATCCATTAG GTTCTAATGG ATCTGGAACA 
AGCGGTACCT CATCCGTAAC TGCGGCGGCA GTCTTGGCAG GAGGATTAAA CTCAGCTAAT 
AAATCCACTC TGCAAGCTCA AAATAAGTCG TCGGCTTTAT TTGCATCGCC TCGAAATTTG 
CGCAAGAGTC AAACATTGCC AGCCCTTAGT AACCCAGAAA AGTCAAATAA AAATGGACCA 
GGTCAACGGC CAGAAAAACA AAAGTTGGCT CAAACTCGTT CAAAGGGTGC CGCTGTCTCA 
ATTGAAGAGA AGGATAGAGA AAGAAAATTG AATGCAAAAA GCCAGCAAAG CAGCACCGAC 
CAAGCCATTA GCAAGGTTAA TGAGTATGAA AACGAGTTCA CTAGCTGGTG CATAAAGAGC 
CTAGATAATA TGTCCGCCAA AGTTGATGTA CCCACGTTCG TTGCATTCTT GCAGGACTTG 
GAAGCGCCAT ATGAGGTAAA GGACTATGTC CGAATATACC TTGGCGATGG AAAAGATTCT 
TTGGATTTCG CAAAGCAGTT TTTGGAGCGA CGTAGCAAAT ACAAAAGCTT GCAACGTGCC 
CAAAAGGCAC ACAATGACGA TATGTGCAAA CCAGCTCCTG CCATTACGCC ATCTGCGAAT 
GACTATGCCG ACAGCAAGAA CAAACAAAAA AAGATTAAAA AGAATAAGAT GACTAAGATG 
GACGCCCGCA TTCTTGGGTT TTCAGTAACT GCTGCCGAGG GTCGCATAAA TGTTGGCATT 
CGAGACTACG TCGAAGGACC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyf ( NM_205878.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995600.2 Drosophila melanogaster gigyf, transcript variant F (Gyf), mRNA.

Price & Availability↑

CloneID	OFa00204
Clone ID Related Accession (Same CDS sequence)	NM_143693.4 , NM_205878.3
Accession Version	NM_205878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gigyf, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_205878.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACCGCT CGCCCAGTGG ATTTGGTAGT 
GCCTCCCGAG GCTCTGGACG TGGTGGAACC GTTGACCGGG GACGGATGCG CGGAAAATCG 
GCCTATCATC CGATATACCA ACGCCCAAGC GGTCTATATG ATGAAAGCTT ATCGGTTATA 
TCAAAGGCCG AACGCACATG GAGCGACCGC AACGGAACTG GAGATTCTGC GGCAACCACC 
ACCTCCACTA GTGGCTCTGG TGCTCTAGAT TGGAATGGAA CTCCAAGCTC AAGTCCTCGA 
AAAGATTATT CTAATAATCA TCGCAACTTG GAAAACTGGC GACGAACACG TAACGAAGAT 
GGATCCGGTG ATGGTCCATC CTCCAGCGGT TCCATGAGCG GACCCGATAT TGCTGGTTGG 
CGGAGCGGTG TCGTCGGTGG AAGTACAAAC GCAGGTTTTG GTACCAATAG CCATCGGTGG 
GGAAGATCTA CAAGCTGGCG TGATGAAGAT CCTAGCGTCG ACAATGCAGC TAGTTTACAA 
CGCTCAATTT CAACAGTTGC GACTTTAAGT ACAGATCGGA CTGGAAACAA TAAAGGATCT 
GGAATCGGAG CTGCAGAGGG AGTAGGGAGC ATATCGCACC CACGGTTGTC GAGTTCTAAA 
ATATCTCAAT TATGGACTGT TAATAATGCA GCCGGTGTTG ATGCCGACGA AAATCTACCC 
GAATGGGCAA TTGAAAATCC ATCGAAATTG GGTGGCAGTT TTGATGCTAG TGGAGCCTTT 
CATGGAGATA CTGATCTAAA ACCTATCAAA AGTTCGCATA ATACTTTAAA AACCAAAAGC 
TTAGACTCCT ATGACGATGT AAAAAGACCG AAAAGTAAAG ACCTGTCTGA TCCAGATAGT 
GGAAACGATA TAACTCCAGA AACCTCATTA ACGAAAGATA CCAATACAAC AGCTGTGCAA 
GAAGAAGTTG AAAGCAGTTT ATCTCCAATA AGCTCTACAA CGACAAAGGA AGTGATCCAT 
GGAGATATTT CAGATCGGAT TAAAGAGGTC GCCGATGAAG TAGAAAAACT TATAATGGAT 
GATGATCATA AAATCTCTTC GAATCAAAGC CAACATCAAA ATCATGACGG GTTTACAGCT 
GCACTTCCAA GAATGGCAGA CATTGAGATA AACGTAAAAC CGAGTGTTAC AGCAGTGCAT 
CGGCAAGCAC CTTCCACCAT GCCTATACAA ATTACCCCTA CAATTACAGA TGTCGCTCCA 
CCATCCCATG CAGTTGTGTC GTTTTCGGAT CATGAGACAA TGCAACACCA TAACATGCAC 
CACCTGCCAC AATTTCCAAT GATTCCTACA CCGCATATCA TCACTCCAAA TCTAAACGAA 
TTGTGGTTTT ACCGGGATCC GCAGGCAAAT GTTCAGGGGC CATTTAGTGC CGTTGAGATG 
ACGGAATGGT ATCGCGCTGG CTACTTTAAT GAGAACCTGT TTGTACGCCG GTACTCTGAT 
AATAGGTTTA GACCGCTGGG AGAGCTTATA AAATTTTGTC ATGGTAACAT GCCATTTACG 
CACAGTCACC TACTTCCATC TCCGATAGAA CTAGAGAACA TTCCTGTTGG TCAAATACCA 
GCCCCTCTAA CGGCGTCACT TTCAATTACA CCCCATAAGC CATCACCGAT TCCTATCGCA 
CTTTCTGTTG TTGAACAGCA GTTGCAGCAA CAAAGAGATG AGCAACTAAA GGCAAATGTT 
ACAGCAACAG CTGAAGCTCT ACGTGCTGCA ATAAAAGGAA GTTTTGGCGG AAATAGCATT 
GGTAATACGT CTCATTTGCT TACAATGCGG TTTCAAATGC TACAGGATCA GTACATACAG 
CACCAGGAAT ACCAAATACT AGCTGAGCTA TCCAAAAATG AATGCTTTCA ACGTCTTTCG 
GCTGTCGAGC AAGAAACAGT TGTTCGTCGG AAAGTTCAAT TGCTGGGTCT TCCTGAGTAT 
TTGATCAGCT TAAACGGATT AAGCAATTCT TTGTCCGTAC TGAACCCCGT TGCCGGTAGA 
CAGTTGTACA GGGCAGTGGT TGAGCACGCC AAGAAGGATC AACAACATAT TTTTGCAAAC 
ACCGAGCAGC AACGTTCAGT GGGAAATTTA CTGGATGCTA ATAATTTCAT TCTAAATGCC 
CAAATAATGA TTCAGCAATC GCAGCAAGAG GTAGGTCCCT TGGTATCATC CGTGGATTGT 
ATTATGCAAG GTGGAACTGC AGCTGACCTA AATAAGCCGA ATGAAATACC GAGGAATGAG 
TTAGACTTAA TTAATGAATA TAACTTACGG ATGCTGTTGC GTGGCCAACC AACAAGTACT 
CAACAGCAAC CACCTGCGCT GACGAACTCA GCTACCGAAA ATCTCCCTGG AGTTGATTTT 
TTAACTGAAA CACAATTGTT AGAGCGGCAA AACTTGATGA TTCCAATCTG GTTACCCCCT 
AATAATAATA AGCAACAAGA AACCGACCAA CAGTGGGCTG AAATGTCTAA TGCGGATGCA 
TCATTATGGG AAACGGCCAA CTTAAATGAA GAGCGAAATG AAGATCAGCA ACTATTAATA 
CAAAAGTCTT CTGAAGCGTG CTTCGCAGAT ACAGAAAAAG ATGTCAAAAT TGCCCAATTA 
TTTCAAGTTC AATCAGGAAA TGTTGTTAAC CACACCGCAT TAGAGGAATT AGATCAGTCC 
CCTCAAAATT TAAAAGGTTC ACATAATCAA AAAATAGTCA AATCCCTTGT TTCCGATATT 
CAACAGAATC ATAATGAGGA ACTAAATTCA CATCAACACC AGGTAAAGCA GGCTAACAAG 
CAGAATCTGA ATACAAAACA GAATGCGGCA CAGTCAGCCC TAAAGCCAAT TAATAACGAA 
AATGATCGCA AACGAGAACA GACAGAAGAA AAAAAAAGGC AAAGAGAGGA ACGCAAGCGC 
CAGCAATTGG AAGATGATAA ACGTCGGGCG TTGAATGAAT CTGAAGAACA GACCCGCCAA 
ATTCAAGAGG AAAAGGAAAG GCAACAGCAA ATACAAGCCC AACGTCGAAA GGCATTGTTG 
GGCAATGTCC ATTCTCTATC AGTTCAAAAT GGGATGTCAG GAACATTAGC GTCTGCACAA 
AGCAAGAAGA ACGACGATGC CAAAACAGCA GAACCCCAAG TATCCTCGCG CTTGCCGTCT 
ACATCCGTAG CGCCTTGGTC TTTTCAGTTG CAAAATTCTA TGAGATCGGC GCCTGGTCTG 
GCAGAAATAC AAAAGGCAGA ACGTCGAGAG CGTCGCGCAG ACCAACAGCG ACATCAAGAG 
CTATTAGACA AGCAATTGCG TGCCAATGCC GCAGCTGCGG CTGAAGCCAA CGATGCTTTG 
CTTAAATGGC AGTCAACACC AGCGTCGGCG CCCGTAATGA GTCTCGCCGA AATTCAAGCA 
GAAGAGGCAA GGCGGTTGGC CAATGACCTT GTGGATCGGC AGCGTCGACG AGAACTGGAA 
CATCACCAAC AAGCTCCTTT GTCATCAGCG GTTTTGGTTA CGAGTGCAAC TTCCAACATC 
TGGGGTAACG CCAATAAAGC ATGGAGTTCG TCCGCTGCTC AATCACTTTC GTTAAAAACA 
AGTTCTGGAA CTGGTCTGTG GGACGAACCG AATCCATTAG GTTCTAATGG ATCTGGAACA 
AGCGGTACCT CATCCGTAAC TGCGGCGGCA GTCTTGGCAG GAGGATTAAA CTCAGCTAAT 
AAATCCACTC TGCAAGCTCA AAATAAGTCG TCGGCTTTAT TTGCATCGCC TCGAAATTTG 
CGCAAGAGTC AAACATTGCC AGCCCTTAGT AACCCAGAAA AGTCAAATAA AAATGGACCA 
GGTCAACGGC CAGAAAAACA AAAGTTGGCT CAAACTCGTT CAAAGGGTGC CGCTGTCTCA 
ATTGAAGAGA AGGATAGAGA AAGAAAATTG AATGCAAAAA GCCAGCAAAG CAGCACCGAC 
CAAGCCATTA GCAAGGTTAA TGAGTATGAA AACGAGTTCA CTAGCTGGTG CATAAAGAGC 
CTAGATAATA TGTCCGCCAA AGTTGATGTA CCCACGTTCG TTGCATTCTT GCAGGACTTG 
GAAGCGCCAT ATGAGGTAAA GGACTATGTC CGAATATACC TTGGCGATGG AAAAGATTCT 
TTGGATTTCG CAAAGCAGTT TTTGGAGCGA CGTAGCAAAT ACAAAAGCTT GCAACGTGCC 
CAAAAGGCAC ACAATGACGA TATGTGCAAA CCAGCTCCTG CCATTACGCC ATCTGCGAAT 
GACTATGCCG ACAGCAAGAA CAAACAAAAA AAGATTAAAA AGAATAAGAT GACTAAGATG 
GACGCCCGCA TTCTTGGGTT TTCAGTAACT GCTGCCGAGG GTCGCATAAA TGTTGGCATT 
CGAGACTACG TCGAAGGACC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995600.2
CDS	85..4788
Translation	MTDSMKFGPEWLRNMSAEPSGSPSTYNVGNAAQNNSIGGHNLGNNTAASASRNLFPEYRYGREEMLSLFDRNCLLPQILPSFKKLFVEKVQCPLALTPSSEEDINQNSLGNNRSPSGFGSASRGSGRGGTVDRGRMRGKSAYHPIYQRPSGLYDESLSVISKAERTWSDRNGTGDSAATTTSTSGSGALDWNGTPSSSPRKDYSNNHRNLENWRRTRNEDGSGDGPSSSGSMSGPDIAGWRSGVVGGSTNAGFGTNSHRWGRSTSWRDEDPSVDNAASLQRSISTVATLSTDRTGNNKGSGIGAAEGVGSISHPRLSSSKISQLWTVNNAAGVDADENLPEWAIENPSKLGGSFDASGAFHGDTDLKPIKSSHNTLKTKSLDSYDDVKRPKSKDLSDPDSGNDITPETSLTKDTNTTAVQEEVESSLSPISSTTTKEVIHGDISDRIKEVADEVEKLIMDDDHKISSNQSQHQNHDGFTAALPRMADIEINVKPSVTAVHRQAPSTMPIQITPTITDVAPPSHAVVSFSDHETMQHHNMHHLPQFPMIPTPHIITPNLNELWFYRDPQANVQGPFSAVEMTEWYRAGYFNENLFVRRYSDNRFRPLGELIKFCHGNMPFTHSHLLPSPIELENIPVGQIPAPLTASLSITPHKPSPIPIALSVVEQQLQQQRDEQLKANVTATAEALRAAIKGSFGGNSIGNTSHLLTMRFQMLQDQYIQHQEYQILAELSKNECFQRLSAVEQETVVRRKVQLLGLPEYLISLNGLSNSLSVLNPVAGRQLYRAVVEHAKKDQQHIFANTEQQRSVGNLLDANNFILNAQIMIQQSQQEVGPLVSSVDCIMQGGTAADLNKPNEIPRNELDLINEYNLRMLLRGQPTSTQQQPPALTNSATENLPGVDFLTETQLLERQNLMIPIWLPPNNNKQQETDQQWAEMSNADASLWETANLNEERNEDQQLLIQKSSEACFADTEKDVKIAQLFQVQSGNVVNHTALEELDQSPQNLKGSHNQKIVKSLVSDIQQNHNEELNSHQHQVKQANKQNLNTKQNAAQSALKPINNENDRKREQTEEKKRQREERKRQQLEDDKRRALNESEEQTRQIQEEKERQQQIQAQRRKALLGNVHSLSVQNGMSGTLASAQSKKNDDAKTAEPQVSSRLPSTSVAPWSFQLQNSMRSAPGLAEIQKAERRERRADQQRHQELLDKQLRANAAAAAEANDALLKWQSTPASAPVMSLAEIQAEEARRLANDLVDRQRRRELEHHQQAPLSSAVLVTSATSNIWGNANKAWSSSAAQSLSLKTSSGTGLWDEPNPLGSNGSGTSGTSSVTAAAVLAGGLNSANKSTLQAQNKSSALFASPRNLRKSQTLPALSNPEKSNKNGPGQRPEKQKLAQTRSKGAAVSIEEKDRERKLNAKSQQSSTDQAISKVNEYENEFTSWCIKSLDNMSAKVDVPTFVAFLQDLEAPYEVKDYVRIYLGDGKDSLDFAKQFLERRSKYKSLQRAQKAHNDDMCKPAPAITPSANDYADSKNKQKKIKKNKMTKMDARILGFSVTAAEGRINVGIRDYVEGP

Target ORF information:

RefSeq Version	NM_205878.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gigyf, transcript variant F (Gyf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205878.3

ORF Insert Sequence:

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACCGCT CGCCCAGTGG ATTTGGTAGT 
GCCTCCCGAG GCTCTGGACG TGGTGGAACC GTTGACCGGG GACGGATGCG CGGAAAATCG 
GCCTATCATC CGATATACCA ACGCCCAAGC GGTCTATATG ATGAAAGCTT ATCGGTTATA 
TCAAAGGCCG AACGCACATG GAGCGACCGC AACGGAACTG GAGATTCTGC GGCAACCACC 
ACCTCCACTA GTGGCTCTGG TGCTCTAGAT TGGAATGGAA CTCCAAGCTC AAGTCCTCGA 
AAAGATTATT CTAATAATCA TCGCAACTTG GAAAACTGGC GACGAACACG TAACGAAGAT 
GGATCCGGTG ATGGTCCATC CTCCAGCGGT TCCATGAGCG GACCCGATAT TGCTGGTTGG 
CGGAGCGGTG TCGTCGGTGG AAGTACAAAC GCAGGTTTTG GTACCAATAG CCATCGGTGG 
GGAAGATCTA CAAGCTGGCG TGATGAAGAT CCTAGCGTCG ACAATGCAGC TAGTTTACAA 
CGCTCAATTT CAACAGTTGC GACTTTAAGT ACAGATCGGA CTGGAAACAA TAAAGGATCT 
GGAATCGGAG CTGCAGAGGG AGTAGGGAGC ATATCGCACC CACGGTTGTC GAGTTCTAAA 
ATATCTCAAT TATGGACTGT TAATAATGCA GCCGGTGTTG ATGCCGACGA AAATCTACCC 
GAATGGGCAA TTGAAAATCC ATCGAAATTG GGTGGCAGTT TTGATGCTAG TGGAGCCTTT 
CATGGAGATA CTGATCTAAA ACCTATCAAA AGTTCGCATA ATACTTTAAA AACCAAAAGC 
TTAGACTCCT ATGACGATGT AAAAAGACCG AAAAGTAAAG ACCTGTCTGA TCCAGATAGT 
GGAAACGATA TAACTCCAGA AACCTCATTA ACGAAAGATA CCAATACAAC AGCTGTGCAA 
GAAGAAGTTG AAAGCAGTTT ATCTCCAATA AGCTCTACAA CGACAAAGGA AGTGATCCAT 
GGAGATATTT CAGATCGGAT TAAAGAGGTC GCCGATGAAG TAGAAAAACT TATAATGGAT 
GATGATCATA AAATCTCTTC GAATCAAAGC CAACATCAAA ATCATGACGG GTTTACAGCT 
GCACTTCCAA GAATGGCAGA CATTGAGATA AACGTAAAAC CGAGTGTTAC AGCAGTGCAT 
CGGCAAGCAC CTTCCACCAT GCCTATACAA ATTACCCCTA CAATTACAGA TGTCGCTCCA 
CCATCCCATG CAGTTGTGTC GTTTTCGGAT CATGAGACAA TGCAACACCA TAACATGCAC 
CACCTGCCAC AATTTCCAAT GATTCCTACA CCGCATATCA TCACTCCAAA TCTAAACGAA 
TTGTGGTTTT ACCGGGATCC GCAGGCAAAT GTTCAGGGGC CATTTAGTGC CGTTGAGATG 
ACGGAATGGT ATCGCGCTGG CTACTTTAAT GAGAACCTGT TTGTACGCCG GTACTCTGAT 
AATAGGTTTA GACCGCTGGG AGAGCTTATA AAATTTTGTC ATGGTAACAT GCCATTTACG 
CACAGTCACC TACTTCCATC TCCGATAGAA CTAGAGAACA TTCCTGTTGG TCAAATACCA 
GCCCCTCTAA CGGCGTCACT TTCAATTACA CCCCATAAGC CATCACCGAT TCCTATCGCA 
CTTTCTGTTG TTGAACAGCA GTTGCAGCAA CAAAGAGATG AGCAACTAAA GGCAAATGTT 
ACAGCAACAG CTGAAGCTCT ACGTGCTGCA ATAAAAGGAA GTTTTGGCGG AAATAGCATT 
GGTAATACGT CTCATTTGCT TACAATGCGG TTTCAAATGC TACAGGATCA GTACATACAG 
CACCAGGAAT ACCAAATACT AGCTGAGCTA TCCAAAAATG AATGCTTTCA ACGTCTTTCG 
GCTGTCGAGC AAGAAACAGT TGTTCGTCGG AAAGTTCAAT TGCTGGGTCT TCCTGAGTAT 
TTGATCAGCT TAAACGGATT AAGCAATTCT TTGTCCGTAC TGAACCCCGT TGCCGGTAGA 
CAGTTGTACA GGGCAGTGGT TGAGCACGCC AAGAAGGATC AACAACATAT TTTTGCAAAC 
ACCGAGCAGC AACGTTCAGT GGGAAATTTA CTGGATGCTA ATAATTTCAT TCTAAATGCC 
CAAATAATGA TTCAGCAATC GCAGCAAGAG GTAGGTCCCT TGGTATCATC CGTGGATTGT 
ATTATGCAAG GTGGAACTGC AGCTGACCTA AATAAGCCGA ATGAAATACC GAGGAATGAG 
TTAGACTTAA TTAATGAATA TAACTTACGG ATGCTGTTGC GTGGCCAACC AACAAGTACT 
CAACAGCAAC CACCTGCGCT GACGAACTCA GCTACCGAAA ATCTCCCTGG AGTTGATTTT 
TTAACTGAAA CACAATTGTT AGAGCGGCAA AACTTGATGA TTCCAATCTG GTTACCCCCT 
AATAATAATA AGCAACAAGA AACCGACCAA CAGTGGGCTG AAATGTCTAA TGCGGATGCA 
TCATTATGGG AAACGGCCAA CTTAAATGAA GAGCGAAATG AAGATCAGCA ACTATTAATA 
CAAAAGTCTT CTGAAGCGTG CTTCGCAGAT ACAGAAAAAG ATGTCAAAAT TGCCCAATTA 
TTTCAAGTTC AATCAGGAAA TGTTGTTAAC CACACCGCAT TAGAGGAATT AGATCAGTCC 
CCTCAAAATT TAAAAGGTTC ACATAATCAA AAAATAGTCA AATCCCTTGT TTCCGATATT 
CAACAGAATC ATAATGAGGA ACTAAATTCA CATCAACACC AGGTAAAGCA GGCTAACAAG 
CAGAATCTGA ATACAAAACA GAATGCGGCA CAGTCAGCCC TAAAGCCAAT TAATAACGAA 
AATGATCGCA AACGAGAACA GACAGAAGAA AAAAAAAGGC AAAGAGAGGA ACGCAAGCGC 
CAGCAATTGG AAGATGATAA ACGTCGGGCG TTGAATGAAT CTGAAGAACA GACCCGCCAA 
ATTCAAGAGG AAAAGGAAAG GCAACAGCAA ATACAAGCCC AACGTCGAAA GGCATTGTTG 
GGCAATGTCC ATTCTCTATC AGTTCAAAAT GGGATGTCAG GAACATTAGC GTCTGCACAA 
AGCAAGAAGA ACGACGATGC CAAAACAGCA GAACCCCAAG TATCCTCGCG CTTGCCGTCT 
ACATCCGTAG CGCCTTGGTC TTTTCAGTTG CAAAATTCTA TGAGATCGGC GCCTGGTCTG 
GCAGAAATAC AAAAGGCAGA ACGTCGAGAG CGTCGCGCAG ACCAACAGCG ACATCAAGAG 
CTATTAGACA AGCAATTGCG TGCCAATGCC GCAGCTGCGG CTGAAGCCAA CGATGCTTTG 
CTTAAATGGC AGTCAACACC AGCGTCGGCG CCCGTAATGA GTCTCGCCGA AATTCAAGCA 
GAAGAGGCAA GGCGGTTGGC CAATGACCTT GTGGATCGGC AGCGTCGACG AGAACTGGAA 
CATCACCAAC AAGCTCCTTT GTCATCAGCG GTTTTGGTTA CGAGTGCAAC TTCCAACATC 
TGGGGTAACG CCAATAAAGC ATGGAGTTCG TCCGCTGCTC AATCACTTTC GTTAAAAACA 
AGTTCTGGAA CTGGTCTGTG GGACGAACCG AATCCATTAG GTTCTAATGG ATCTGGAACA 
AGCGGTACCT CATCCGTAAC TGCGGCGGCA GTCTTGGCAG GAGGATTAAA CTCAGCTAAT 
AAATCCACTC TGCAAGCTCA AAATAAGTCG TCGGCTTTAT TTGCATCGCC TCGAAATTTG 
CGCAAGAGTC AAACATTGCC AGCCCTTAGT AACCCAGAAA AGTCAAATAA AAATGGACCA 
GGTCAACGGC CAGAAAAACA AAAGTTGGCT CAAACTCGTT CAAAGGGTGC CGCTGTCTCA 
ATTGAAGAGA AGGATAGAGA AAGAAAATTG AATGCAAAAA GCCAGCAAAG CAGCACCGAC 
CAAGCCATTA GCAAGGTTAA TGAGTATGAA AACGAGTTCA CTAGCTGGTG CATAAAGAGC 
CTAGATAATA TGTCCGCCAA AGTTGATGTA CCCACGTTCG TTGCATTCTT GCAGGACTTG 
GAAGCGCCAT ATGAGGTAAA GGACTATGTC CGAATATACC TTGGCGATGG AAAAGATTCT 
TTGGATTTCG CAAAGCAGTT TTTGGAGCGA CGTAGCAAAT ACAAAAGCTT GCAACGTGCC 
CAAAAGGCAC ACAATGACGA TATGTGCAAA CCAGCTCCTG CCATTACGCC ATCTGCGAAT 
GACTATGCCG ACAGCAAGAA CAAACAAAAA AAGATTAAAA AGAATAAGAT GACTAAGATG 
GACGCCCGCA TTCTTGGGTT TTCAGTAACT GCTGCCGAGG GTCGCATAAA TGTTGGCATT 
CGAGACTACG TCGAAGGACC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyf ( NM_001169357.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162828.1 Drosophila melanogaster gigyf, transcript variant H (Gyf), mRNA.

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CloneID	OFa00319
Clone ID Related Accession (Same CDS sequence)	NM_001169357.4 , NM_001169356.2
Accession Version	NM_001169357.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gigyf, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Oct 19, 2015 this sequence version replaced NM_001169357.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACTCTC GTCCTGCCTG GTTGCAGCGC 
TCGCCCAGTG GATTTGGTAG TGCCTCCCGA GGCTCTGGAC GTGGTGGAAC CGTTGACCGG 
GGACGGATGC GCGGAAAATC GGCCTATCAT CCGATATACC AACGCCCAAG CGGTCTATAT 
GATGAAAGCT TATCGGTTAT ATCAAAGGCC GAACGCACAT GGAGCGACCG CAACGGAACT 
GGAGATTCTG CGGCAACCAC CACCTCCACT AGTGGCTCTG GTGCTCTAGA TTGGAATGGA 
ACTCCAAGCT CAAGTCCTCG AAAAGATTAT TCTAATAATC ATCGCAACTT GGAAAACTGG 
CGACGAACAC GTAACGAAGA TGGATCCGGT GATGGTCCAT CCTCCAGCGG TTCCATGAGC 
GGACCCGATA TTGCTGGTTG GCGGAGCGGT GTCGTCGGTG GAAGTACAAA CGCAGGTTTT 
GGTACCAATA GCCATCGGTG GGGAAGATCT ACAAGCTGGC GTGATGAAGA TCCTAGCGTC 
GACAATGCAG CTAGTTTACA ACGCTCAATT TCAACAGTTG CGACTTTAAG TACAGATCGG 
ACTGGAAACA ATAAAGGATC TGGAATCGGA GCTGCAGAGG GAGTAGGGAG CATATCGCAC 
CCACGGTTGT CGAGTTCTAA AATATCTCAA TTATGGACTG TTAATAATGC AGCCGGTGTT 
GATGCCGACG AAAATCTACC CGAATGGGCA ATTGAAAATC CATCGAAATT GGGTGGCAGT 
TTTGATGCTA GTGGAGCCTT TCATGGAGAT ACTGATCTAA AACCTATCAA AAGTTCGCAT 
AATACTTTAA AAACCAAAAG CTTAGACTCC TATGACGATG TAAAAAGACC GAAAAGTAAA 
GACCTGTCTG ATCCAGATAG TGGAAACGAT ATAACTCCAG AAACCTCATT AACGAAAGAT 
ACCAATACAA CAGCTGTGCA AGAAGAAGTT GAAAGCAGTT TATCTCCAAT AAGCTCTACA 
ACGACAAAGG AAGTGATCCA TGGAGATATT TCAGATCGGA TTAAAGAGGT CGCCGATGAA 
GTAGAAAAAC TTATAATGGA TGATGATCAT AAAATCTCTT CGAATCAAAG CCAACATCAA 
AATCATGACG GGTTTACAGC TGCACTTCCA AGAATGGCAG ACATTGAGAT AAACGTAAAA 
CCGAGTGTTA CAGCAGTGCA TCGGCAAGCA CCTTCCACCA TGCCTATACA AATTACCCCT 
ACAATTACAG ATGTCGCTCC ACCATCCCAT GCAGTTGTGT CGTTTTCGGA TCATGAGACA 
ATGCAACACC ATAACATGCA CCACCTGCCA CAATTTCCAA TGATTCCTAC ACCGCATATC 
ATCACTCCAA ATCTAAACGA ATTGTGGTTT TACCGGGATC CGCAGGCAAA TGTTCAGGGG 
CCATTTAGTG CCGTTGAGAT GACGGAATGG TATCGCGCTG GCTACTTTAA TGAGAACCTG 
TTTGTACGCC GGTACTCTGA TAATAGGTTT AGACCGCTGG GAGAGCTTAT AAAATTTTGT 
CATGGTAACA TGCCATTTAC GCACAGTCAC CTACTTCCAT CTCCGATAGA ACTAGAGAAC 
ATTCCTGTTG GTCAAATACC AGCCCCTCTA ACGGCGTCAC TTTCAATTAC ACCCCATAAG 
CCATCACCGA TTCCTATCGC ACTTTCTGTT GTTGAACAGC AGTTGCAGCA ACAAAGAGAT 
GAGCAACTAA AGGCAAATGT TACAGCAACA GCTGAAGCTC TACGTGCTGC AATAAAAGGA 
AGTTTTGGCG GAAATAGCAT TGGTAATACG TCTCATTTGC TTACAATGCG GTTTCAAATG 
CTACAGGATC AGTACATACA GCACCAGGAA TACCAAATAC TAGCTGAGCT ATCCAAAAAT 
GAATGCTTTC AACGTCTTTC GGCTGTCGAG CAAGAAACAG TTGTTCGTCG GAAAGTTCAA 
TTGCTGGGTC TTCCTGAGTA TTTGATCAGC TTAAACGGAT TAAGCAATTC TTTGTCCGTA 
CTGAACCCCG TTGCCGGTAG ACAGTTGTAC AGGGCAGTGG TTGAGCACGC CAAGAAGGAT 
CAACAACATA TTTTTGCAAA CACCGAGCAG CAACGTTCAG TGGGAAATTT ACTGGATGCT 
AATAATTTCA TTCTAAATGC CCAAATAATG ATTCAGCAAT CGCAGCAAGA GGTAGGTCCC 
TTGGTATCAT CCGTGGATTG TATTATGCAA GGTGGAACTG CAGCTGACCT AAATAAGCCG 
AATGAAATAC CGAGGAATGA GTTAGACTTA ATTAATGAAT ATAACTTACG GATGCTGTTG 
CGTGGCCAAC CAACAAGTAC TCAACAGCAA CCACCTGCGC TGACGAACTC AGCTACCGAA 
AATCTCCCTG GAGTTGATTT TTTAACTGAA ACACAATTGT TAGAGCGGCA AAACTTGATG 
ATTCCAATCT GGTTACCCCC TAATAATAAT AAGCAACAAG AAACCGACCA ACAGTGGGCT 
GAAATGTCTA ATGCGGATGC ATCATTATGG GAAACGGCCA ACTTAAATGA AGAGCGAAAT 
GAAGATCAGC AACTATTAAT ACAAAAGTCT TCTGAAGCGT GCTTCGCAGA TACAGAAAAA 
GATGTCAAAA TTGCCCAATT ATTTCAAGTT CAATCAGGAA ATGTTGTTAA CCACACCGCA 
TTAGAGGAAT TAGATCAGTC CCCTCAAAAT TTAAAAGGTT CACATAATCA AAAAATAGTC 
AAATCCCTTG TTTCCGATAT TCAACAGAAT CATAATGAGG AACTAAATTC ACATCAACAC 
CAGGTAAAGC AGGCTAACAA GCAGAATCTG AATACAAAAC AGAATGCGGC ACAGTCAGCC 
CTAAAGCCAA TTAATAACGA AAATGATCGC AAACGAGAAC AGACAGAAGA AAAAAAAAGG 
CAAAGAGAGG AACGCAAGCG CCAGCAATTG GAAGATGATA AACGTCGGGC GTTGAATGAA 
TCTGAAGAAC AGACCCGCCA AATTCAAGAG GAAAAGGAAA GGCAACAGCA AATACAAGCC 
CAACGTCGAA AGGCATTGTT GGGCAATGTC CATTCTCTAT CAGTTCAAAA TGGGATGTCA 
GGAACATTAG CGTCTGCACA AAGCAAGAAG AACGACGATG CCAAAACAGC AGAACCCCAA 
GTATCCTCGC GCTTGCCGTC TACATCCGTA GCGCCTTGGT CTTTTCAGTT GCAAAATTCT 
ATGAGATCGG CGCCTGGTCT GGCAGAAATA CAAAAGGCAG AACGTCGAGA GCGTCGCGCA 
GACCAACAGC GACATCAAGA GCTATTAGAC AAGCAATTGC GTGCCAATGC CGCAGCTGCG 
GCTGAAGCCA ACGATGCTTT GCTTAAATGG CAGTCAACAC CAGCGTCGGC GCCCGTAATG 
AGTCTCGCCG AAATTCAAGC AGAAGAGGCA AGGCGGTTGG CCAATGACCT TGTGGATCGG 
CAGCGTCGAC GAGAACTGGA ACATCACCAA CAAGCTCCTT TGTCATCAGC GGTTTTGGTT 
ACGAGTGCAA CTTCCAACAT CTGGGGTAAC GCCAATAAAG CATGGAGTTC GTCCGCTGCT 
CAATCACTTT CGTTAAAAAC AAGTTCTGGA ACTGGTCTGT GGGACGAACC GAATCCATTA 
GGTTCTAATG GATCTGGAAC AAGCGGTACC TCATCCGTAA CTGCGGCGGC AGTCTTGGCA 
GGAGGATTAA ACTCAGCTAA TAAATCCACT CTGCAAGCTC AAAATAAGTC GTCGGCTTTA 
TTTGCATCGC CTCGAAATTT GCGCAAGAGT CAAACATTGC CAGCCCTTAG TAACCCAGAA 
AAGTCAAATA AAAATGGACC AGGTCAACGG CCAGAAAAAC AAAAGTTGGC TCAAACTCGT 
TCAAAGGGTG CCGCTGTCTC AATTGAAGAG AAGGATAGAG AAAGAAAATT GAATGCAAAA 
AGCCAGCAAA GCAGCACCGA CCAAGCCATT AGCAAGGTTA ATGAGTATGA AAACGAGTTC 
ACTAGCTGGT GCATAAAGAG CCTAGATAAT ATGTCCGCCA AAGTTGATGT ACCCACGTTC 
GTTGCATTCT TGCAGGACTT GGAAGCGCCA TATGAGGTAA AGGACTATGT CCGAATATAC 
CTTGGCGATG GAAAAGATTC TTTGGATTTC GCAAAGCAGT TTTTGGAGCG ACGTAGCAAA 
TACAAAAGCT TGCAACGTGC CCAAAAGGCA CACAATGACG ATATGTGCAA ACCAGCTCCT 
GCCATTACGC CATCTGCGAA TGACTATGCC GACAGCAAGA ACAAACAAAA AAAGATTAAA 
AAGAATAAGA TGACTAAGAT GGACGCCCGC ATTCTTGGGT TTTCAGTAAC TGCTGCCGAG 
GGTCGCATAA ATGTTGGCAT TCGAGACTAC GTCGAAGGAC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162828.1
CDS	79..4803
Translation	MTDSMKFGPEWLRNMSAEPSGSPSTYNVGNAAQNNSIGGHNLGNNTAASASRNLFPEYRYGREEMLSLFDRNCLLPQILPSFKKLFVEKVQCPLALTPSSEEDINQNSLGNNSRPAWLQRSPSGFGSASRGSGRGGTVDRGRMRGKSAYHPIYQRPSGLYDESLSVISKAERTWSDRNGTGDSAATTTSTSGSGALDWNGTPSSSPRKDYSNNHRNLENWRRTRNEDGSGDGPSSSGSMSGPDIAGWRSGVVGGSTNAGFGTNSHRWGRSTSWRDEDPSVDNAASLQRSISTVATLSTDRTGNNKGSGIGAAEGVGSISHPRLSSSKISQLWTVNNAAGVDADENLPEWAIENPSKLGGSFDASGAFHGDTDLKPIKSSHNTLKTKSLDSYDDVKRPKSKDLSDPDSGNDITPETSLTKDTNTTAVQEEVESSLSPISSTTTKEVIHGDISDRIKEVADEVEKLIMDDDHKISSNQSQHQNHDGFTAALPRMADIEINVKPSVTAVHRQAPSTMPIQITPTITDVAPPSHAVVSFSDHETMQHHNMHHLPQFPMIPTPHIITPNLNELWFYRDPQANVQGPFSAVEMTEWYRAGYFNENLFVRRYSDNRFRPLGELIKFCHGNMPFTHSHLLPSPIELENIPVGQIPAPLTASLSITPHKPSPIPIALSVVEQQLQQQRDEQLKANVTATAEALRAAIKGSFGGNSIGNTSHLLTMRFQMLQDQYIQHQEYQILAELSKNECFQRLSAVEQETVVRRKVQLLGLPEYLISLNGLSNSLSVLNPVAGRQLYRAVVEHAKKDQQHIFANTEQQRSVGNLLDANNFILNAQIMIQQSQQEVGPLVSSVDCIMQGGTAADLNKPNEIPRNELDLINEYNLRMLLRGQPTSTQQQPPALTNSATENLPGVDFLTETQLLERQNLMIPIWLPPNNNKQQETDQQWAEMSNADASLWETANLNEERNEDQQLLIQKSSEACFADTEKDVKIAQLFQVQSGNVVNHTALEELDQSPQNLKGSHNQKIVKSLVSDIQQNHNEELNSHQHQVKQANKQNLNTKQNAAQSALKPINNENDRKREQTEEKKRQREERKRQQLEDDKRRALNESEEQTRQIQEEKERQQQIQAQRRKALLGNVHSLSVQNGMSGTLASAQSKKNDDAKTAEPQVSSRLPSTSVAPWSFQLQNSMRSAPGLAEIQKAERRERRADQQRHQELLDKQLRANAAAAAEANDALLKWQSTPASAPVMSLAEIQAEEARRLANDLVDRQRRRELEHHQQAPLSSAVLVTSATSNIWGNANKAWSSSAAQSLSLKTSSGTGLWDEPNPLGSNGSGTSGTSSVTAAAVLAGGLNSANKSTLQAQNKSSALFASPRNLRKSQTLPALSNPEKSNKNGPGQRPEKQKLAQTRSKGAAVSIEEKDRERKLNAKSQQSSTDQAISKVNEYENEFTSWCIKSLDNMSAKVDVPTFVAFLQDLEAPYEVKDYVRIYLGDGKDSLDFAKQFLERRSKYKSLQRAQKAHNDDMCKPAPAITPSANDYADSKNKQKKIKKNKMTKMDARILGFSVTAAEGRINVGIRDYVEGP

Target ORF information:

RefSeq Version	NM_001169357.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gigyf, transcript variant H (Gyf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169357.4

ORF Insert Sequence:

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACTCTC GTCCTGCCTG GTTGCAGCGC 
TCGCCCAGTG GATTTGGTAG TGCCTCCCGA GGCTCTGGAC GTGGTGGAAC CGTTGACCGG 
GGACGGATGC GCGGAAAATC GGCCTATCAT CCGATATACC AACGCCCAAG CGGTCTATAT 
GATGAAAGCT TATCGGTTAT ATCAAAGGCC GAACGCACAT GGAGCGACCG CAACGGAACT 
GGAGATTCTG CGGCAACCAC CACCTCCACT AGTGGCTCTG GTGCTCTAGA TTGGAATGGA 
ACTCCAAGCT CAAGTCCTCG AAAAGATTAT TCTAATAATC ATCGCAACTT GGAAAACTGG 
CGACGAACAC GTAACGAAGA TGGATCCGGT GATGGTCCAT CCTCCAGCGG TTCCATGAGC 
GGACCCGATA TTGCTGGTTG GCGGAGCGGT GTCGTCGGTG GAAGTACAAA CGCAGGTTTT 
GGTACCAATA GCCATCGGTG GGGAAGATCT ACAAGCTGGC GTGATGAAGA TCCTAGCGTC 
GACAATGCAG CTAGTTTACA ACGCTCAATT TCAACAGTTG CGACTTTAAG TACAGATCGG 
ACTGGAAACA ATAAAGGATC TGGAATCGGA GCTGCAGAGG GAGTAGGGAG CATATCGCAC 
CCACGGTTGT CGAGTTCTAA AATATCTCAA TTATGGACTG TTAATAATGC AGCCGGTGTT 
GATGCCGACG AAAATCTACC CGAATGGGCA ATTGAAAATC CATCGAAATT GGGTGGCAGT 
TTTGATGCTA GTGGAGCCTT TCATGGAGAT ACTGATCTAA AACCTATCAA AAGTTCGCAT 
AATACTTTAA AAACCAAAAG CTTAGACTCC TATGACGATG TAAAAAGACC GAAAAGTAAA 
GACCTGTCTG ATCCAGATAG TGGAAACGAT ATAACTCCAG AAACCTCATT AACGAAAGAT 
ACCAATACAA CAGCTGTGCA AGAAGAAGTT GAAAGCAGTT TATCTCCAAT AAGCTCTACA 
ACGACAAAGG AAGTGATCCA TGGAGATATT TCAGATCGGA TTAAAGAGGT CGCCGATGAA 
GTAGAAAAAC TTATAATGGA TGATGATCAT AAAATCTCTT CGAATCAAAG CCAACATCAA 
AATCATGACG GGTTTACAGC TGCACTTCCA AGAATGGCAG ACATTGAGAT AAACGTAAAA 
CCGAGTGTTA CAGCAGTGCA TCGGCAAGCA CCTTCCACCA TGCCTATACA AATTACCCCT 
ACAATTACAG ATGTCGCTCC ACCATCCCAT GCAGTTGTGT CGTTTTCGGA TCATGAGACA 
ATGCAACACC ATAACATGCA CCACCTGCCA CAATTTCCAA TGATTCCTAC ACCGCATATC 
ATCACTCCAA ATCTAAACGA ATTGTGGTTT TACCGGGATC CGCAGGCAAA TGTTCAGGGG 
CCATTTAGTG CCGTTGAGAT GACGGAATGG TATCGCGCTG GCTACTTTAA TGAGAACCTG 
TTTGTACGCC GGTACTCTGA TAATAGGTTT AGACCGCTGG GAGAGCTTAT AAAATTTTGT 
CATGGTAACA TGCCATTTAC GCACAGTCAC CTACTTCCAT CTCCGATAGA ACTAGAGAAC 
ATTCCTGTTG GTCAAATACC AGCCCCTCTA ACGGCGTCAC TTTCAATTAC ACCCCATAAG 
CCATCACCGA TTCCTATCGC ACTTTCTGTT GTTGAACAGC AGTTGCAGCA ACAAAGAGAT 
GAGCAACTAA AGGCAAATGT TACAGCAACA GCTGAAGCTC TACGTGCTGC AATAAAAGGA 
AGTTTTGGCG GAAATAGCAT TGGTAATACG TCTCATTTGC TTACAATGCG GTTTCAAATG 
CTACAGGATC AGTACATACA GCACCAGGAA TACCAAATAC TAGCTGAGCT ATCCAAAAAT 
GAATGCTTTC AACGTCTTTC GGCTGTCGAG CAAGAAACAG TTGTTCGTCG GAAAGTTCAA 
TTGCTGGGTC TTCCTGAGTA TTTGATCAGC TTAAACGGAT TAAGCAATTC TTTGTCCGTA 
CTGAACCCCG TTGCCGGTAG ACAGTTGTAC AGGGCAGTGG TTGAGCACGC CAAGAAGGAT 
CAACAACATA TTTTTGCAAA CACCGAGCAG CAACGTTCAG TGGGAAATTT ACTGGATGCT 
AATAATTTCA TTCTAAATGC CCAAATAATG ATTCAGCAAT CGCAGCAAGA GGTAGGTCCC 
TTGGTATCAT CCGTGGATTG TATTATGCAA GGTGGAACTG CAGCTGACCT AAATAAGCCG 
AATGAAATAC CGAGGAATGA GTTAGACTTA ATTAATGAAT ATAACTTACG GATGCTGTTG 
CGTGGCCAAC CAACAAGTAC TCAACAGCAA CCACCTGCGC TGACGAACTC AGCTACCGAA 
AATCTCCCTG GAGTTGATTT TTTAACTGAA ACACAATTGT TAGAGCGGCA AAACTTGATG 
ATTCCAATCT GGTTACCCCC TAATAATAAT AAGCAACAAG AAACCGACCA ACAGTGGGCT 
GAAATGTCTA ATGCGGATGC ATCATTATGG GAAACGGCCA ACTTAAATGA AGAGCGAAAT 
GAAGATCAGC AACTATTAAT ACAAAAGTCT TCTGAAGCGT GCTTCGCAGA TACAGAAAAA 
GATGTCAAAA TTGCCCAATT ATTTCAAGTT CAATCAGGAA ATGTTGTTAA CCACACCGCA 
TTAGAGGAAT TAGATCAGTC CCCTCAAAAT TTAAAAGGTT CACATAATCA AAAAATAGTC 
AAATCCCTTG TTTCCGATAT TCAACAGAAT CATAATGAGG AACTAAATTC ACATCAACAC 
CAGGTAAAGC AGGCTAACAA GCAGAATCTG AATACAAAAC AGAATGCGGC ACAGTCAGCC 
CTAAAGCCAA TTAATAACGA AAATGATCGC AAACGAGAAC AGACAGAAGA AAAAAAAAGG 
CAAAGAGAGG AACGCAAGCG CCAGCAATTG GAAGATGATA AACGTCGGGC GTTGAATGAA 
TCTGAAGAAC AGACCCGCCA AATTCAAGAG GAAAAGGAAA GGCAACAGCA AATACAAGCC 
CAACGTCGAA AGGCATTGTT GGGCAATGTC CATTCTCTAT CAGTTCAAAA TGGGATGTCA 
GGAACATTAG CGTCTGCACA AAGCAAGAAG AACGACGATG CCAAAACAGC AGAACCCCAA 
GTATCCTCGC GCTTGCCGTC TACATCCGTA GCGCCTTGGT CTTTTCAGTT GCAAAATTCT 
ATGAGATCGG CGCCTGGTCT GGCAGAAATA CAAAAGGCAG AACGTCGAGA GCGTCGCGCA 
GACCAACAGC GACATCAAGA GCTATTAGAC AAGCAATTGC GTGCCAATGC CGCAGCTGCG 
GCTGAAGCCA ACGATGCTTT GCTTAAATGG CAGTCAACAC CAGCGTCGGC GCCCGTAATG 
AGTCTCGCCG AAATTCAAGC AGAAGAGGCA AGGCGGTTGG CCAATGACCT TGTGGATCGG 
CAGCGTCGAC GAGAACTGGA ACATCACCAA CAAGCTCCTT TGTCATCAGC GGTTTTGGTT 
ACGAGTGCAA CTTCCAACAT CTGGGGTAAC GCCAATAAAG CATGGAGTTC GTCCGCTGCT 
CAATCACTTT CGTTAAAAAC AAGTTCTGGA ACTGGTCTGT GGGACGAACC GAATCCATTA 
GGTTCTAATG GATCTGGAAC AAGCGGTACC TCATCCGTAA CTGCGGCGGC AGTCTTGGCA 
GGAGGATTAA ACTCAGCTAA TAAATCCACT CTGCAAGCTC AAAATAAGTC GTCGGCTTTA 
TTTGCATCGC CTCGAAATTT GCGCAAGAGT CAAACATTGC CAGCCCTTAG TAACCCAGAA 
AAGTCAAATA AAAATGGACC AGGTCAACGG CCAGAAAAAC AAAAGTTGGC TCAAACTCGT 
TCAAAGGGTG CCGCTGTCTC AATTGAAGAG AAGGATAGAG AAAGAAAATT GAATGCAAAA 
AGCCAGCAAA GCAGCACCGA CCAAGCCATT AGCAAGGTTA ATGAGTATGA AAACGAGTTC 
ACTAGCTGGT GCATAAAGAG CCTAGATAAT ATGTCCGCCA AAGTTGATGT ACCCACGTTC 
GTTGCATTCT TGCAGGACTT GGAAGCGCCA TATGAGGTAA AGGACTATGT CCGAATATAC 
CTTGGCGATG GAAAAGATTC TTTGGATTTC GCAAAGCAGT TTTTGGAGCG ACGTAGCAAA 
TACAAAAGCT TGCAACGTGC CCAAAAGGCA CACAATGACG ATATGTGCAA ACCAGCTCCT 
GCCATTACGC CATCTGCGAA TGACTATGCC GACAGCAAGA ACAAACAAAA AAAGATTAAA 
AAGAATAAGA TGACTAAGAT GGACGCCCGC ATTCTTGGGT TTTCAGTAAC TGCTGCCGAG 
GGTCGCATAA ATGTTGGCAT TCGAGACTAC GTCGAAGGAC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyf ( NM_001169356.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162827.1 Drosophila melanogaster gigyf, transcript variant G (Gyf), mRNA.

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CloneID	OFa00319
Clone ID Related Accession (Same CDS sequence)	NM_001169357.4 , NM_001169356.2
Accession Version	NM_001169356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	gigyf, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001169356.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACTCTC GTCCTGCCTG GTTGCAGCGC 
TCGCCCAGTG GATTTGGTAG TGCCTCCCGA GGCTCTGGAC GTGGTGGAAC CGTTGACCGG 
GGACGGATGC GCGGAAAATC GGCCTATCAT CCGATATACC AACGCCCAAG CGGTCTATAT 
GATGAAAGCT TATCGGTTAT ATCAAAGGCC GAACGCACAT GGAGCGACCG CAACGGAACT 
GGAGATTCTG CGGCAACCAC CACCTCCACT AGTGGCTCTG GTGCTCTAGA TTGGAATGGA 
ACTCCAAGCT CAAGTCCTCG AAAAGATTAT TCTAATAATC ATCGCAACTT GGAAAACTGG 
CGACGAACAC GTAACGAAGA TGGATCCGGT GATGGTCCAT CCTCCAGCGG TTCCATGAGC 
GGACCCGATA TTGCTGGTTG GCGGAGCGGT GTCGTCGGTG GAAGTACAAA CGCAGGTTTT 
GGTACCAATA GCCATCGGTG GGGAAGATCT ACAAGCTGGC GTGATGAAGA TCCTAGCGTC 
GACAATGCAG CTAGTTTACA ACGCTCAATT TCAACAGTTG CGACTTTAAG TACAGATCGG 
ACTGGAAACA ATAAAGGATC TGGAATCGGA GCTGCAGAGG GAGTAGGGAG CATATCGCAC 
CCACGGTTGT CGAGTTCTAA AATATCTCAA TTATGGACTG TTAATAATGC AGCCGGTGTT 
GATGCCGACG AAAATCTACC CGAATGGGCA ATTGAAAATC CATCGAAATT GGGTGGCAGT 
TTTGATGCTA GTGGAGCCTT TCATGGAGAT ACTGATCTAA AACCTATCAA AAGTTCGCAT 
AATACTTTAA AAACCAAAAG CTTAGACTCC TATGACGATG TAAAAAGACC GAAAAGTAAA 
GACCTGTCTG ATCCAGATAG TGGAAACGAT ATAACTCCAG AAACCTCATT AACGAAAGAT 
ACCAATACAA CAGCTGTGCA AGAAGAAGTT GAAAGCAGTT TATCTCCAAT AAGCTCTACA 
ACGACAAAGG AAGTGATCCA TGGAGATATT TCAGATCGGA TTAAAGAGGT CGCCGATGAA 
GTAGAAAAAC TTATAATGGA TGATGATCAT AAAATCTCTT CGAATCAAAG CCAACATCAA 
AATCATGACG GGTTTACAGC TGCACTTCCA AGAATGGCAG ACATTGAGAT AAACGTAAAA 
CCGAGTGTTA CAGCAGTGCA TCGGCAAGCA CCTTCCACCA TGCCTATACA AATTACCCCT 
ACAATTACAG ATGTCGCTCC ACCATCCCAT GCAGTTGTGT CGTTTTCGGA TCATGAGACA 
ATGCAACACC ATAACATGCA CCACCTGCCA CAATTTCCAA TGATTCCTAC ACCGCATATC 
ATCACTCCAA ATCTAAACGA ATTGTGGTTT TACCGGGATC CGCAGGCAAA TGTTCAGGGG 
CCATTTAGTG CCGTTGAGAT GACGGAATGG TATCGCGCTG GCTACTTTAA TGAGAACCTG 
TTTGTACGCC GGTACTCTGA TAATAGGTTT AGACCGCTGG GAGAGCTTAT AAAATTTTGT 
CATGGTAACA TGCCATTTAC GCACAGTCAC CTACTTCCAT CTCCGATAGA ACTAGAGAAC 
ATTCCTGTTG GTCAAATACC AGCCCCTCTA ACGGCGTCAC TTTCAATTAC ACCCCATAAG 
CCATCACCGA TTCCTATCGC ACTTTCTGTT GTTGAACAGC AGTTGCAGCA ACAAAGAGAT 
GAGCAACTAA AGGCAAATGT TACAGCAACA GCTGAAGCTC TACGTGCTGC AATAAAAGGA 
AGTTTTGGCG GAAATAGCAT TGGTAATACG TCTCATTTGC TTACAATGCG GTTTCAAATG 
CTACAGGATC AGTACATACA GCACCAGGAA TACCAAATAC TAGCTGAGCT ATCCAAAAAT 
GAATGCTTTC AACGTCTTTC GGCTGTCGAG CAAGAAACAG TTGTTCGTCG GAAAGTTCAA 
TTGCTGGGTC TTCCTGAGTA TTTGATCAGC TTAAACGGAT TAAGCAATTC TTTGTCCGTA 
CTGAACCCCG TTGCCGGTAG ACAGTTGTAC AGGGCAGTGG TTGAGCACGC CAAGAAGGAT 
CAACAACATA TTTTTGCAAA CACCGAGCAG CAACGTTCAG TGGGAAATTT ACTGGATGCT 
AATAATTTCA TTCTAAATGC CCAAATAATG ATTCAGCAAT CGCAGCAAGA GGTAGGTCCC 
TTGGTATCAT CCGTGGATTG TATTATGCAA GGTGGAACTG CAGCTGACCT AAATAAGCCG 
AATGAAATAC CGAGGAATGA GTTAGACTTA ATTAATGAAT ATAACTTACG GATGCTGTTG 
CGTGGCCAAC CAACAAGTAC TCAACAGCAA CCACCTGCGC TGACGAACTC AGCTACCGAA 
AATCTCCCTG GAGTTGATTT TTTAACTGAA ACACAATTGT TAGAGCGGCA AAACTTGATG 
ATTCCAATCT GGTTACCCCC TAATAATAAT AAGCAACAAG AAACCGACCA ACAGTGGGCT 
GAAATGTCTA ATGCGGATGC ATCATTATGG GAAACGGCCA ACTTAAATGA AGAGCGAAAT 
GAAGATCAGC AACTATTAAT ACAAAAGTCT TCTGAAGCGT GCTTCGCAGA TACAGAAAAA 
GATGTCAAAA TTGCCCAATT ATTTCAAGTT CAATCAGGAA ATGTTGTTAA CCACACCGCA 
TTAGAGGAAT TAGATCAGTC CCCTCAAAAT TTAAAAGGTT CACATAATCA AAAAATAGTC 
AAATCCCTTG TTTCCGATAT TCAACAGAAT CATAATGAGG AACTAAATTC ACATCAACAC 
CAGGTAAAGC AGGCTAACAA GCAGAATCTG AATACAAAAC AGAATGCGGC ACAGTCAGCC 
CTAAAGCCAA TTAATAACGA AAATGATCGC AAACGAGAAC AGACAGAAGA AAAAAAAAGG 
CAAAGAGAGG AACGCAAGCG CCAGCAATTG GAAGATGATA AACGTCGGGC GTTGAATGAA 
TCTGAAGAAC AGACCCGCCA AATTCAAGAG GAAAAGGAAA GGCAACAGCA AATACAAGCC 
CAACGTCGAA AGGCATTGTT GGGCAATGTC CATTCTCTAT CAGTTCAAAA TGGGATGTCA 
GGAACATTAG CGTCTGCACA AAGCAAGAAG AACGACGATG CCAAAACAGC AGAACCCCAA 
GTATCCTCGC GCTTGCCGTC TACATCCGTA GCGCCTTGGT CTTTTCAGTT GCAAAATTCT 
ATGAGATCGG CGCCTGGTCT GGCAGAAATA CAAAAGGCAG AACGTCGAGA GCGTCGCGCA 
GACCAACAGC GACATCAAGA GCTATTAGAC AAGCAATTGC GTGCCAATGC CGCAGCTGCG 
GCTGAAGCCA ACGATGCTTT GCTTAAATGG CAGTCAACAC CAGCGTCGGC GCCCGTAATG 
AGTCTCGCCG AAATTCAAGC AGAAGAGGCA AGGCGGTTGG CCAATGACCT TGTGGATCGG 
CAGCGTCGAC GAGAACTGGA ACATCACCAA CAAGCTCCTT TGTCATCAGC GGTTTTGGTT 
ACGAGTGCAA CTTCCAACAT CTGGGGTAAC GCCAATAAAG CATGGAGTTC GTCCGCTGCT 
CAATCACTTT CGTTAAAAAC AAGTTCTGGA ACTGGTCTGT GGGACGAACC GAATCCATTA 
GGTTCTAATG GATCTGGAAC AAGCGGTACC TCATCCGTAA CTGCGGCGGC AGTCTTGGCA 
GGAGGATTAA ACTCAGCTAA TAAATCCACT CTGCAAGCTC AAAATAAGTC GTCGGCTTTA 
TTTGCATCGC CTCGAAATTT GCGCAAGAGT CAAACATTGC CAGCCCTTAG TAACCCAGAA 
AAGTCAAATA AAAATGGACC AGGTCAACGG CCAGAAAAAC AAAAGTTGGC TCAAACTCGT 
TCAAAGGGTG CCGCTGTCTC AATTGAAGAG AAGGATAGAG AAAGAAAATT GAATGCAAAA 
AGCCAGCAAA GCAGCACCGA CCAAGCCATT AGCAAGGTTA ATGAGTATGA AAACGAGTTC 
ACTAGCTGGT GCATAAAGAG CCTAGATAAT ATGTCCGCCA AAGTTGATGT ACCCACGTTC 
GTTGCATTCT TGCAGGACTT GGAAGCGCCA TATGAGGTAA AGGACTATGT CCGAATATAC 
CTTGGCGATG GAAAAGATTC TTTGGATTTC GCAAAGCAGT TTTTGGAGCG ACGTAGCAAA 
TACAAAAGCT TGCAACGTGC CCAAAAGGCA CACAATGACG ATATGTGCAA ACCAGCTCCT 
GCCATTACGC CATCTGCGAA TGACTATGCC GACAGCAAGA ACAAACAAAA AAAGATTAAA 
AAGAATAAGA TGACTAAGAT GGACGCCCGC ATTCTTGGGT TTTCAGTAAC TGCTGCCGAG 
GGTCGCATAA ATGTTGGCAT TCGAGACTAC GTCGAAGGAC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162827.1
CDS	85..4809
Translation	MTDSMKFGPEWLRNMSAEPSGSPSTYNVGNAAQNNSIGGHNLGNNTAASASRNLFPEYRYGREEMLSLFDRNCLLPQILPSFKKLFVEKVQCPLALTPSSEEDINQNSLGNNSRPAWLQRSPSGFGSASRGSGRGGTVDRGRMRGKSAYHPIYQRPSGLYDESLSVISKAERTWSDRNGTGDSAATTTSTSGSGALDWNGTPSSSPRKDYSNNHRNLENWRRTRNEDGSGDGPSSSGSMSGPDIAGWRSGVVGGSTNAGFGTNSHRWGRSTSWRDEDPSVDNAASLQRSISTVATLSTDRTGNNKGSGIGAAEGVGSISHPRLSSSKISQLWTVNNAAGVDADENLPEWAIENPSKLGGSFDASGAFHGDTDLKPIKSSHNTLKTKSLDSYDDVKRPKSKDLSDPDSGNDITPETSLTKDTNTTAVQEEVESSLSPISSTTTKEVIHGDISDRIKEVADEVEKLIMDDDHKISSNQSQHQNHDGFTAALPRMADIEINVKPSVTAVHRQAPSTMPIQITPTITDVAPPSHAVVSFSDHETMQHHNMHHLPQFPMIPTPHIITPNLNELWFYRDPQANVQGPFSAVEMTEWYRAGYFNENLFVRRYSDNRFRPLGELIKFCHGNMPFTHSHLLPSPIELENIPVGQIPAPLTASLSITPHKPSPIPIALSVVEQQLQQQRDEQLKANVTATAEALRAAIKGSFGGNSIGNTSHLLTMRFQMLQDQYIQHQEYQILAELSKNECFQRLSAVEQETVVRRKVQLLGLPEYLISLNGLSNSLSVLNPVAGRQLYRAVVEHAKKDQQHIFANTEQQRSVGNLLDANNFILNAQIMIQQSQQEVGPLVSSVDCIMQGGTAADLNKPNEIPRNELDLINEYNLRMLLRGQPTSTQQQPPALTNSATENLPGVDFLTETQLLERQNLMIPIWLPPNNNKQQETDQQWAEMSNADASLWETANLNEERNEDQQLLIQKSSEACFADTEKDVKIAQLFQVQSGNVVNHTALEELDQSPQNLKGSHNQKIVKSLVSDIQQNHNEELNSHQHQVKQANKQNLNTKQNAAQSALKPINNENDRKREQTEEKKRQREERKRQQLEDDKRRALNESEEQTRQIQEEKERQQQIQAQRRKALLGNVHSLSVQNGMSGTLASAQSKKNDDAKTAEPQVSSRLPSTSVAPWSFQLQNSMRSAPGLAEIQKAERRERRADQQRHQELLDKQLRANAAAAAEANDALLKWQSTPASAPVMSLAEIQAEEARRLANDLVDRQRRRELEHHQQAPLSSAVLVTSATSNIWGNANKAWSSSAAQSLSLKTSSGTGLWDEPNPLGSNGSGTSGTSSVTAAAVLAGGLNSANKSTLQAQNKSSALFASPRNLRKSQTLPALSNPEKSNKNGPGQRPEKQKLAQTRSKGAAVSIEEKDRERKLNAKSQQSSTDQAISKVNEYENEFTSWCIKSLDNMSAKVDVPTFVAFLQDLEAPYEVKDYVRIYLGDGKDSLDFAKQFLERRSKYKSLQRAQKAHNDDMCKPAPAITPSANDYADSKNKQKKIKKNKMTKMDARILGFSVTAAEGRINVGIRDYVEGP

Target ORF information:

RefSeq Version	NM_001169356.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster gigyf, transcript variant G (Gyf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169356.2

ORF Insert Sequence:

ATGACAGATT CAATGAAATT TGGCCCGGAA TGGTTGCGCA ATATGTCAGC CGAGCCTTCG 
GGCTCTCCCA GTACCTACAA CGTTGGTAAT GCAGCTCAAA ACAATTCGAT TGGAGGGCAC 
AACCTGGGAA ACAACACGGC GGCATCCGCT TCCCGTAATC TATTTCCAGA ATACCGGTAC 
GGACGCGAGG AAATGCTGTC CTTGTTCGAT CGGAACTGCC TACTGCCGCA GATCCTGCCA 
TCGTTCAAAA AGCTCTTCGT GGAAAAGGTT CAGTGCCCTC TTGCACTGAC ACCGAGCTCC 
GAGGAGGACA TCAACCAAAA CTCACTTGGC AATAACTCTC GTCCTGCCTG GTTGCAGCGC 
TCGCCCAGTG GATTTGGTAG TGCCTCCCGA GGCTCTGGAC GTGGTGGAAC CGTTGACCGG 
GGACGGATGC GCGGAAAATC GGCCTATCAT CCGATATACC AACGCCCAAG CGGTCTATAT 
GATGAAAGCT TATCGGTTAT ATCAAAGGCC GAACGCACAT GGAGCGACCG CAACGGAACT 
GGAGATTCTG CGGCAACCAC CACCTCCACT AGTGGCTCTG GTGCTCTAGA TTGGAATGGA 
ACTCCAAGCT CAAGTCCTCG AAAAGATTAT TCTAATAATC ATCGCAACTT GGAAAACTGG 
CGACGAACAC GTAACGAAGA TGGATCCGGT GATGGTCCAT CCTCCAGCGG TTCCATGAGC 
GGACCCGATA TTGCTGGTTG GCGGAGCGGT GTCGTCGGTG GAAGTACAAA CGCAGGTTTT 
GGTACCAATA GCCATCGGTG GGGAAGATCT ACAAGCTGGC GTGATGAAGA TCCTAGCGTC 
GACAATGCAG CTAGTTTACA ACGCTCAATT TCAACAGTTG CGACTTTAAG TACAGATCGG 
ACTGGAAACA ATAAAGGATC TGGAATCGGA GCTGCAGAGG GAGTAGGGAG CATATCGCAC 
CCACGGTTGT CGAGTTCTAA AATATCTCAA TTATGGACTG TTAATAATGC AGCCGGTGTT 
GATGCCGACG AAAATCTACC CGAATGGGCA ATTGAAAATC CATCGAAATT GGGTGGCAGT 
TTTGATGCTA GTGGAGCCTT TCATGGAGAT ACTGATCTAA AACCTATCAA AAGTTCGCAT 
AATACTTTAA AAACCAAAAG CTTAGACTCC TATGACGATG TAAAAAGACC GAAAAGTAAA 
GACCTGTCTG ATCCAGATAG TGGAAACGAT ATAACTCCAG AAACCTCATT AACGAAAGAT 
ACCAATACAA CAGCTGTGCA AGAAGAAGTT GAAAGCAGTT TATCTCCAAT AAGCTCTACA 
ACGACAAAGG AAGTGATCCA TGGAGATATT TCAGATCGGA TTAAAGAGGT CGCCGATGAA 
GTAGAAAAAC TTATAATGGA TGATGATCAT AAAATCTCTT CGAATCAAAG CCAACATCAA 
AATCATGACG GGTTTACAGC TGCACTTCCA AGAATGGCAG ACATTGAGAT AAACGTAAAA 
CCGAGTGTTA CAGCAGTGCA TCGGCAAGCA CCTTCCACCA TGCCTATACA AATTACCCCT 
ACAATTACAG ATGTCGCTCC ACCATCCCAT GCAGTTGTGT CGTTTTCGGA TCATGAGACA 
ATGCAACACC ATAACATGCA CCACCTGCCA CAATTTCCAA TGATTCCTAC ACCGCATATC 
ATCACTCCAA ATCTAAACGA ATTGTGGTTT TACCGGGATC CGCAGGCAAA TGTTCAGGGG 
CCATTTAGTG CCGTTGAGAT GACGGAATGG TATCGCGCTG GCTACTTTAA TGAGAACCTG 
TTTGTACGCC GGTACTCTGA TAATAGGTTT AGACCGCTGG GAGAGCTTAT AAAATTTTGT 
CATGGTAACA TGCCATTTAC GCACAGTCAC CTACTTCCAT CTCCGATAGA ACTAGAGAAC 
ATTCCTGTTG GTCAAATACC AGCCCCTCTA ACGGCGTCAC TTTCAATTAC ACCCCATAAG 
CCATCACCGA TTCCTATCGC ACTTTCTGTT GTTGAACAGC AGTTGCAGCA ACAAAGAGAT 
GAGCAACTAA AGGCAAATGT TACAGCAACA GCTGAAGCTC TACGTGCTGC AATAAAAGGA 
AGTTTTGGCG GAAATAGCAT TGGTAATACG TCTCATTTGC TTACAATGCG GTTTCAAATG 
CTACAGGATC AGTACATACA GCACCAGGAA TACCAAATAC TAGCTGAGCT ATCCAAAAAT 
GAATGCTTTC AACGTCTTTC GGCTGTCGAG CAAGAAACAG TTGTTCGTCG GAAAGTTCAA 
TTGCTGGGTC TTCCTGAGTA TTTGATCAGC TTAAACGGAT TAAGCAATTC TTTGTCCGTA 
CTGAACCCCG TTGCCGGTAG ACAGTTGTAC AGGGCAGTGG TTGAGCACGC CAAGAAGGAT 
CAACAACATA TTTTTGCAAA CACCGAGCAG CAACGTTCAG TGGGAAATTT ACTGGATGCT 
AATAATTTCA TTCTAAATGC CCAAATAATG ATTCAGCAAT CGCAGCAAGA GGTAGGTCCC 
TTGGTATCAT CCGTGGATTG TATTATGCAA GGTGGAACTG CAGCTGACCT AAATAAGCCG 
AATGAAATAC CGAGGAATGA GTTAGACTTA ATTAATGAAT ATAACTTACG GATGCTGTTG 
CGTGGCCAAC CAACAAGTAC TCAACAGCAA CCACCTGCGC TGACGAACTC AGCTACCGAA 
AATCTCCCTG GAGTTGATTT TTTAACTGAA ACACAATTGT TAGAGCGGCA AAACTTGATG 
ATTCCAATCT GGTTACCCCC TAATAATAAT AAGCAACAAG AAACCGACCA ACAGTGGGCT 
GAAATGTCTA ATGCGGATGC ATCATTATGG GAAACGGCCA ACTTAAATGA AGAGCGAAAT 
GAAGATCAGC AACTATTAAT ACAAAAGTCT TCTGAAGCGT GCTTCGCAGA TACAGAAAAA 
GATGTCAAAA TTGCCCAATT ATTTCAAGTT CAATCAGGAA ATGTTGTTAA CCACACCGCA 
TTAGAGGAAT TAGATCAGTC CCCTCAAAAT TTAAAAGGTT CACATAATCA AAAAATAGTC 
AAATCCCTTG TTTCCGATAT TCAACAGAAT CATAATGAGG AACTAAATTC ACATCAACAC 
CAGGTAAAGC AGGCTAACAA GCAGAATCTG AATACAAAAC AGAATGCGGC ACAGTCAGCC 
CTAAAGCCAA TTAATAACGA AAATGATCGC AAACGAGAAC AGACAGAAGA AAAAAAAAGG 
CAAAGAGAGG AACGCAAGCG CCAGCAATTG GAAGATGATA AACGTCGGGC GTTGAATGAA 
TCTGAAGAAC AGACCCGCCA AATTCAAGAG GAAAAGGAAA GGCAACAGCA AATACAAGCC 
CAACGTCGAA AGGCATTGTT GGGCAATGTC CATTCTCTAT CAGTTCAAAA TGGGATGTCA 
GGAACATTAG CGTCTGCACA AAGCAAGAAG AACGACGATG CCAAAACAGC AGAACCCCAA 
GTATCCTCGC GCTTGCCGTC TACATCCGTA GCGCCTTGGT CTTTTCAGTT GCAAAATTCT 
ATGAGATCGG CGCCTGGTCT GGCAGAAATA CAAAAGGCAG AACGTCGAGA GCGTCGCGCA 
GACCAACAGC GACATCAAGA GCTATTAGAC AAGCAATTGC GTGCCAATGC CGCAGCTGCG 
GCTGAAGCCA ACGATGCTTT GCTTAAATGG CAGTCAACAC CAGCGTCGGC GCCCGTAATG 
AGTCTCGCCG AAATTCAAGC AGAAGAGGCA AGGCGGTTGG CCAATGACCT TGTGGATCGG 
CAGCGTCGAC GAGAACTGGA ACATCACCAA CAAGCTCCTT TGTCATCAGC GGTTTTGGTT 
ACGAGTGCAA CTTCCAACAT CTGGGGTAAC GCCAATAAAG CATGGAGTTC GTCCGCTGCT 
CAATCACTTT CGTTAAAAAC AAGTTCTGGA ACTGGTCTGT GGGACGAACC GAATCCATTA 
GGTTCTAATG GATCTGGAAC AAGCGGTACC TCATCCGTAA CTGCGGCGGC AGTCTTGGCA 
GGAGGATTAA ACTCAGCTAA TAAATCCACT CTGCAAGCTC AAAATAAGTC GTCGGCTTTA 
TTTGCATCGC CTCGAAATTT GCGCAAGAGT CAAACATTGC CAGCCCTTAG TAACCCAGAA 
AAGTCAAATA AAAATGGACC AGGTCAACGG CCAGAAAAAC AAAAGTTGGC TCAAACTCGT 
TCAAAGGGTG CCGCTGTCTC AATTGAAGAG AAGGATAGAG AAAGAAAATT GAATGCAAAA 
AGCCAGCAAA GCAGCACCGA CCAAGCCATT AGCAAGGTTA ATGAGTATGA AAACGAGTTC 
ACTAGCTGGT GCATAAAGAG CCTAGATAAT ATGTCCGCCA AAGTTGATGT ACCCACGTTC 
GTTGCATTCT TGCAGGACTT GGAAGCGCCA TATGAGGTAA AGGACTATGT CCGAATATAC 
CTTGGCGATG GAAAAGATTC TTTGGATTTC GCAAAGCAGT TTTTGGAGCG ACGTAGCAAA 
TACAAAAGCT TGCAACGTGC CCAAAAGGCA CACAATGACG ATATGTGCAA ACCAGCTCCT 
GCCATTACGC CATCTGCGAA TGACTATGCC GACAGCAAGA ACAAACAAAA AAAGATTAAA 
AAGAATAAGA TGACTAAGAT GGACGCCCGC ATTCTTGGGT TTTCAGTAAC TGCTGCCGAG 
GGTCGCATAA ATGTTGGCAT TCGAGACTAC GTCGAAGGAC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gyf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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