pho cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pho
Entrez Gene ID	43819
Full Name	pleiohomeotic
Synonyms	CG17743,Dmel\CG17743,PHO,PHO/YY1,Pho,YY1,dYY1,l(4)102EFc,l(4)29,l(4)BU-2,l(4)OC-1,l(4)pho
General protein information	Preferred Names pleiohomeotic Names CG17743 gene product from transcript CG17743-RB CG17743-PA CG17743-PB PHO peiohomeotic pho-PA pho-PB pleihomeotic pleohomeotic polyhomeotic
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102F8-102F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_166827.2	NP_726651.1	pleiohomeotic, isoform B
NM_079891.3	NP_524630.1	pleiohomeotic, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spt5 interacts both physically and genetically with the Polycomb Group (PcG) protein Pleiohomeotic (Pho)
Title: Pleiohomeotic interacts with the core transcription elongation factor Spt5 to regulate gene expression in Drosophila.

even-skipped genes were misexpressed in pho mutant embryos
Title: The pleiohomeotic functions as a negative regulator of Drosophila even-skipped gene during embryogenesis.

The TF homolog pair Pho/Yy1 seems to play very different roles in PcG targeting between Drosophila and mouse.
Title: Prediction of Polycomb target genes in mouse embryonic stem cells.

CtBP regulates expression of intergenic transcripts that controls DNA binding by PcG proteins and subsequent histone modifications and transcriptional activity.
Title: CtBP levels control intergenic transcripts, PHO/YY1 DNA binding, and PcG recruitment to DNA.

a DNA-binding complex requiring Pho is differentially regulated to facilitate epigenetic transcriptional memory of both the active and the repressed state
Title: The DNA-binding Polycomb-group protein Pleiohomeotic maintains both active and repressed transcriptional states through a single site.

The following pho gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pho cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00297	NM_166827.2 Latest version!	Drosophila melanogaster pleiohomeotic (pho), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00297	NM_079891.3 Latest version!	Drosophila melanogaster pleiohomeotic (pho), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pho ( NM_166827.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726651.1 Drosophila melanogaster pleiohomeotic (pho), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00297
Clone ID Related Accession (Same CDS sequence)	NM_166827.2 , NM_079891.3
Accession Version	NM_166827.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pleiohomeotic, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166827.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATACG AACGTTTTGG AATTATTCTT CAAAGTGAAC AGTACGATGA AGATATAGGC 
AACACAAAAG TCAATCAGAA AATGAATGAA GGCAACCACT ACGACTTACA CAGAAAAAAT 
GCGTTTGACC GAATTATTCA TTCAGAATCA AAAAAAGGTG ATAACGTAAT TAATTACAAC 
ATTCATGAAA ACGACAAAAT AAAAGCGGCT GACAATATAT TTTCTTCTAA ATTAAAAATG 
AACCCGAATA TGAGCTATGA AATGAATATC AACTGTTTTA AAAATATTGG ATATGGCGAA 
AATCAAGAAA CTTCAAAAGT TTTAACAAAT AGTTTATCGA ACAATGATAT TAATACCGAA 
GAGTCTGGTG TTGTCGATAA AAATTCACCT TTCCTAACCT TAGGTACAAC TATACTTAAT 
TCAAACGGCA AGTCAAGACG GTGGGAACAA AAGCTTGTTC ATATTAAAAC TATGGAGGGA 
GAGTTTAGTG TTACAATGTG GGCATCCGGG ATATCAGACG ATGAATACTC TGGTTCAGAT 
CAAATCGTTG GGGCTTCGGA TTTATTAAAA GGAAAGGAAG AATTTGGCAT TGATGGCTTC 
ACGTCTCAAC AAAATAAAGA ATACCAGAAA ATGGAGTCAA AATTTACAAA TGCACAGACC 
CTTGAAATGC CGCATCCTAT AAGCTCAGTT CAGATCATGG ATCATTTAAT AAAGGAAAGG 
GGAAATTTAT CGCAAGAAAA TAATATTTCC GAAAGAATTT TATCTAAGAC CACCTTAAGC 
TTTGAAGAGC CAATCTTGCT TCCGGATTCT TCAAGTATTG AGTTAGTTAA CGAAACTGCC 
GCAATGACAA TTAATAATCA CAGAACTTTA AGTAATCATA CGGGCAACAC CGGTGATCTG 
CATGCTTTGC CCAGCTCAGT GCCATTTCGT ATTGGCTTGC ATGAGGGGCA AGTAAATGAT 
TGCTTATCAA CAATATCACA ATCAACTCAC CAAGACAATA CAGACTCGAC TGGATGTGGG 
GAAATGAATC TTTCCGAAGT TACAGTATCT TATACAAATG ATAAAAAAAT AGCTTGCCCA 
CATAAGGGTT GTAATAAGCA CTTCAGAGAT TCATCTGCAA TGCGAAAACA TTTGCACACT 
CACGGTCCTC GAGTCCATGT TTGTGCAGAA TGTGGAAAAG CTTTCGTAGA AAGCTCAAAG 
CTTAAAAGAC ATCAGTTGGT TCACACCGGT GAAAAACCAT TTCAATGTAC ATTCGAGGGA 
TGCGGAAAAC GATTTTCTCT GGATTTTAAC TTAAGAACAC ACGTAAGAAT ACATACTGGA 
GATAGACCAT TTGTTTGTCC GTTTGATGCC TGTAATAAAA AATTTGCTCA ATCCACGAAT 
TTAAAATCGC ACATATTAAC TCACGCAAAA GCAAAGAGAA ATACCAGTAT TTCGGGGAAA 
AGTGGTTGTT CAAACGCTGA ATCAAACAGC CAGAGTGAAG ATACCTCTGC AAACTATGTT 
AAGGTTGAGT TACAGGATAG TGTTACAGAA AACCATGTAC CGTTTGTGGT ATATGCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726651.1
CDS	183..1745
Translation	MAYERFGIILQSEQYDEDIGNTKVNQKMNEGNHYDLHRKNAFDRIIHSESKKGDNVINYNIHENDKIKAADNIFSSKLKMNPNMSYEMNINCFKNIGYGENQETSKVLTNSLSNNDINTEESGVVDKNSPFLTLGTTILNSNGKSRRWEQKLVHIKTMEGEFSVTMWASGISDDEYSGSDQIVGASDLLKGKEEFGIDGFTSQQNKEYQKMESKFTNAQTLEMPHPISSVQIMDHLIKERGNLSQENNISERILSKTTLSFEEPILLPDSSSIELVNETAAMTINNHRTLSNHTGNTGDLHALPSSVPFRIGLHEGQVNDCLSTISQSTHQDNTDSTGCGEMNLSEVTVSYTNDKKIACPHKGCNKHFRDSSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPFVCPFDACNKKFAQSTNLKSHILTHAKAKRNTSISGKSGCSNAESNSQSEDTSANYVKVELQDSVTENHVPFVVYAD

Target ORF information:

RefSeq Version	NM_166827.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pleiohomeotic (pho), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166827.2

ORF Insert Sequence:

ATGGCATACG AACGTTTTGG AATTATTCTT CAAAGTGAAC AGTACGATGA AGATATAGGC 
AACACAAAAG TCAATCAGAA AATGAATGAA GGCAACCACT ACGACTTACA CAGAAAAAAT 
GCGTTTGACC GAATTATTCA TTCAGAATCA AAAAAAGGTG ATAACGTAAT TAATTACAAC 
ATTCATGAAA ACGACAAAAT AAAAGCGGCT GACAATATAT TTTCTTCTAA ATTAAAAATG 
AACCCGAATA TGAGCTATGA AATGAATATC AACTGTTTTA AAAATATTGG ATATGGCGAA 
AATCAAGAAA CTTCAAAAGT TTTAACAAAT AGTTTATCGA ACAATGATAT TAATACCGAA 
GAGTCTGGTG TTGTCGATAA AAATTCACCT TTCCTAACCT TAGGTACAAC TATACTTAAT 
TCAAACGGCA AGTCAAGACG GTGGGAACAA AAGCTTGTTC ATATTAAAAC TATGGAGGGA 
GAGTTTAGTG TTACAATGTG GGCATCCGGG ATATCAGACG ATGAATACTC TGGTTCAGAT 
CAAATCGTTG GGGCTTCGGA TTTATTAAAA GGAAAGGAAG AATTTGGCAT TGATGGCTTC 
ACGTCTCAAC AAAATAAAGA ATACCAGAAA ATGGAGTCAA AATTTACAAA TGCACAGACC 
CTTGAAATGC CGCATCCTAT AAGCTCAGTT CAGATCATGG ATCATTTAAT AAAGGAAAGG 
GGAAATTTAT CGCAAGAAAA TAATATTTCC GAAAGAATTT TATCTAAGAC CACCTTAAGC 
TTTGAAGAGC CAATCTTGCT TCCGGATTCT TCAAGTATTG AGTTAGTTAA CGAAACTGCC 
GCAATGACAA TTAATAATCA CAGAACTTTA AGTAATCATA CGGGCAACAC CGGTGATCTG 
CATGCTTTGC CCAGCTCAGT GCCATTTCGT ATTGGCTTGC ATGAGGGGCA AGTAAATGAT 
TGCTTATCAA CAATATCACA ATCAACTCAC CAAGACAATA CAGACTCGAC TGGATGTGGG 
GAAATGAATC TTTCCGAAGT TACAGTATCT TATACAAATG ATAAAAAAAT AGCTTGCCCA 
CATAAGGGTT GTAATAAGCA CTTCAGAGAT TCATCTGCAA TGCGAAAACA TTTGCACACT 
CACGGTCCTC GAGTCCATGT TTGTGCAGAA TGTGGAAAAG CTTTCGTAGA AAGCTCAAAG 
CTTAAAAGAC ATCAGTTGGT TCACACCGGT GAAAAACCAT TTCAATGTAC ATTCGAGGGA 
TGCGGAAAAC GATTTTCTCT GGATTTTAAC TTAAGAACAC ACGTAAGAAT ACATACTGGA 
GATAGACCAT TTGTTTGTCC GTTTGATGCC TGTAATAAAA AATTTGCTCA ATCCACGAAT 
TTAAAATCGC ACATATTAAC TCACGCAAAA GCAAAGAGAA ATACCAGTAT TTCGGGGAAA 
AGTGGTTGTT CAAACGCTGA ATCAAACAGC CAGAGTGAAG ATACCTCTGC AAACTATGTT 
AAGGTTGAGT TACAGGATAG TGTTACAGAA AACCATGTAC CGTTTGTGGT ATATGCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pho ( NM_079891.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524630.1 Drosophila melanogaster pleiohomeotic (pho), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00297
Clone ID Related Accession (Same CDS sequence)	NM_166827.2 , NM_079891.3
Accession Version	NM_079891.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pleiohomeotic, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_079891.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCATACG AACGTTTTGG AATTATTCTT CAAAGTGAAC AGTACGATGA AGATATAGGC 
AACACAAAAG TCAATCAGAA AATGAATGAA GGCAACCACT ACGACTTACA CAGAAAAAAT 
GCGTTTGACC GAATTATTCA TTCAGAATCA AAAAAAGGTG ATAACGTAAT TAATTACAAC 
ATTCATGAAA ACGACAAAAT AAAAGCGGCT GACAATATAT TTTCTTCTAA ATTAAAAATG 
AACCCGAATA TGAGCTATGA AATGAATATC AACTGTTTTA AAAATATTGG ATATGGCGAA 
AATCAAGAAA CTTCAAAAGT TTTAACAAAT AGTTTATCGA ACAATGATAT TAATACCGAA 
GAGTCTGGTG TTGTCGATAA AAATTCACCT TTCCTAACCT TAGGTACAAC TATACTTAAT 
TCAAACGGCA AGTCAAGACG GTGGGAACAA AAGCTTGTTC ATATTAAAAC TATGGAGGGA 
GAGTTTAGTG TTACAATGTG GGCATCCGGG ATATCAGACG ATGAATACTC TGGTTCAGAT 
CAAATCGTTG GGGCTTCGGA TTTATTAAAA GGAAAGGAAG AATTTGGCAT TGATGGCTTC 
ACGTCTCAAC AAAATAAAGA ATACCAGAAA ATGGAGTCAA AATTTACAAA TGCACAGACC 
CTTGAAATGC CGCATCCTAT AAGCTCAGTT CAGATCATGG ATCATTTAAT AAAGGAAAGG 
GGAAATTTAT CGCAAGAAAA TAATATTTCC GAAAGAATTT TATCTAAGAC CACCTTAAGC 
TTTGAAGAGC CAATCTTGCT TCCGGATTCT TCAAGTATTG AGTTAGTTAA CGAAACTGCC 
GCAATGACAA TTAATAATCA CAGAACTTTA AGTAATCATA CGGGCAACAC CGGTGATCTG 
CATGCTTTGC CCAGCTCAGT GCCATTTCGT ATTGGCTTGC ATGAGGGGCA AGTAAATGAT 
TGCTTATCAA CAATATCACA ATCAACTCAC CAAGACAATA CAGACTCGAC TGGATGTGGG 
GAAATGAATC TTTCCGAAGT TACAGTATCT TATACAAATG ATAAAAAAAT AGCTTGCCCA 
CATAAGGGTT GTAATAAGCA CTTCAGAGAT TCATCTGCAA TGCGAAAACA TTTGCACACT 
CACGGTCCTC GAGTCCATGT TTGTGCAGAA TGTGGAAAAG CTTTCGTAGA AAGCTCAAAG 
CTTAAAAGAC ATCAGTTGGT TCACACCGGT GAAAAACCAT TTCAATGTAC ATTCGAGGGA 
TGCGGAAAAC GATTTTCTCT GGATTTTAAC TTAAGAACAC ACGTAAGAAT ACATACTGGA 
GATAGACCAT TTGTTTGTCC GTTTGATGCC TGTAATAAAA AATTTGCTCA ATCCACGAAT 
TTAAAATCGC ACATATTAAC TCACGCAAAA GCAAAGAGAA ATACCAGTAT TTCGGGGAAA 
AGTGGTTGTT CAAACGCTGA ATCAAACAGC CAGAGTGAAG ATACCTCTGC AAACTATGTT 
AAGGTTGAGT TACAGGATAG TGTTACAGAA AACCATGTAC CGTTTGTGGT ATATGCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524630.1
CDS	405..1967
Translation	MAYERFGIILQSEQYDEDIGNTKVNQKMNEGNHYDLHRKNAFDRIIHSESKKGDNVINYNIHENDKIKAADNIFSSKLKMNPNMSYEMNINCFKNIGYGENQETSKVLTNSLSNNDINTEESGVVDKNSPFLTLGTTILNSNGKSRRWEQKLVHIKTMEGEFSVTMWASGISDDEYSGSDQIVGASDLLKGKEEFGIDGFTSQQNKEYQKMESKFTNAQTLEMPHPISSVQIMDHLIKERGNLSQENNISERILSKTTLSFEEPILLPDSSSIELVNETAAMTINNHRTLSNHTGNTGDLHALPSSVPFRIGLHEGQVNDCLSTISQSTHQDNTDSTGCGEMNLSEVTVSYTNDKKIACPHKGCNKHFRDSSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPFVCPFDACNKKFAQSTNLKSHILTHAKAKRNTSISGKSGCSNAESNSQSEDTSANYVKVELQDSVTENHVPFVVYAD

Target ORF information:

RefSeq Version	NM_079891.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pleiohomeotic (pho), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079891.3

ORF Insert Sequence:

ATGGCATACG AACGTTTTGG AATTATTCTT CAAAGTGAAC AGTACGATGA AGATATAGGC 
AACACAAAAG TCAATCAGAA AATGAATGAA GGCAACCACT ACGACTTACA CAGAAAAAAT 
GCGTTTGACC GAATTATTCA TTCAGAATCA AAAAAAGGTG ATAACGTAAT TAATTACAAC 
ATTCATGAAA ACGACAAAAT AAAAGCGGCT GACAATATAT TTTCTTCTAA ATTAAAAATG 
AACCCGAATA TGAGCTATGA AATGAATATC AACTGTTTTA AAAATATTGG ATATGGCGAA 
AATCAAGAAA CTTCAAAAGT TTTAACAAAT AGTTTATCGA ACAATGATAT TAATACCGAA 
GAGTCTGGTG TTGTCGATAA AAATTCACCT TTCCTAACCT TAGGTACAAC TATACTTAAT 
TCAAACGGCA AGTCAAGACG GTGGGAACAA AAGCTTGTTC ATATTAAAAC TATGGAGGGA 
GAGTTTAGTG TTACAATGTG GGCATCCGGG ATATCAGACG ATGAATACTC TGGTTCAGAT 
CAAATCGTTG GGGCTTCGGA TTTATTAAAA GGAAAGGAAG AATTTGGCAT TGATGGCTTC 
ACGTCTCAAC AAAATAAAGA ATACCAGAAA ATGGAGTCAA AATTTACAAA TGCACAGACC 
CTTGAAATGC CGCATCCTAT AAGCTCAGTT CAGATCATGG ATCATTTAAT AAAGGAAAGG 
GGAAATTTAT CGCAAGAAAA TAATATTTCC GAAAGAATTT TATCTAAGAC CACCTTAAGC 
TTTGAAGAGC CAATCTTGCT TCCGGATTCT TCAAGTATTG AGTTAGTTAA CGAAACTGCC 
GCAATGACAA TTAATAATCA CAGAACTTTA AGTAATCATA CGGGCAACAC CGGTGATCTG 
CATGCTTTGC CCAGCTCAGT GCCATTTCGT ATTGGCTTGC ATGAGGGGCA AGTAAATGAT 
TGCTTATCAA CAATATCACA ATCAACTCAC CAAGACAATA CAGACTCGAC TGGATGTGGG 
GAAATGAATC TTTCCGAAGT TACAGTATCT TATACAAATG ATAAAAAAAT AGCTTGCCCA 
CATAAGGGTT GTAATAAGCA CTTCAGAGAT TCATCTGCAA TGCGAAAACA TTTGCACACT 
CACGGTCCTC GAGTCCATGT TTGTGCAGAA TGTGGAAAAG CTTTCGTAGA AAGCTCAAAG 
CTTAAAAGAC ATCAGTTGGT TCACACCGGT GAAAAACCAT TTCAATGTAC ATTCGAGGGA 
TGCGGAAAAC GATTTTCTCT GGATTTTAAC TTAAGAACAC ACGTAAGAAT ACATACTGGA 
GATAGACCAT TTGTTTGTCC GTTTGATGCC TGTAATAAAA AATTTGCTCA ATCCACGAAT 
TTAAAATCGC ACATATTAAC TCACGCAAAA GCAAAGAGAA ATACCAGTAT TTCGGGGAAA 
AGTGGTTGTT CAAACGCTGA ATCAAACAGC CAGAGTGAAG ATACCTCTGC AAACTATGTT 
AAGGTTGAGT TACAGGATAG TGTTACAGAA AACCATGTAC CGTTTGTGGT ATATGCAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pho cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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