RhoGAP102A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	RhoGAP102A
Entrez Gene ID	43781
Full Name	Rho GTPase activating protein at 102A
Synonyms	BP1050,CG1748,CG34013,CG42316,Dm_4:1183,Dmel\CG42316,Dmel_CG1748,Dmel_CG34013,mlncRNA102B1,rhoGAP102A
General protein information	Preferred Names Rho GTPase activating protein at 102A Names CG42316 gene product from transcript CG42316-RD CG42316-PC CG42316-PD CG42316-PE CG42316-PF RhoGAP102A-PC RhoGAP102A-PD RhoGAP102A-PE RhoGAP102A-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102B1-102B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_143657.5	NP_651914.3	Rho GTPase activating protein at 102A, isoform E
NM_001258487.3	NP_001245416.1	Rho GTPase activating protein at 102A, isoform D
NM_001038713.4	NP_001033802.2	Rho GTPase activating protein at 102A, isoform C
NM_001272121.2	NP_001259050.1	Rho GTPase activating protein at 102A, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RhoGAP102A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RhoGAP102A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00148	NM_001258487.3 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa00149	NM_001272121.2 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa00249	NM_143657.5 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00250	NM_001038713.4 Latest version!	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RhoGAP102A ( NM_001258487.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245416.1 Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00148
Clone ID Related Accession (Same CDS sequence)	NM_001258487.3
Accession Version	NM_001258487.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 102A, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_001258487.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGAA CCATGATAAA TCACTATGAA 
GAAATTTTCA ACATATCAGC AGAGTTACTG AATGTCATTT ATACACAAGC ACTGGAAGCT 
TGTCCGGAAA AGTTATATGA GCTGATTTCC ACGAAAGTCT ATGGAACTGA ATGGACCCAA 
CAGCAAATAG ACGACCCTCA ACCAGGGAGT TTATCTGATG TTTTGCTCGA ACCTCCGATC 
CACGGAAAGC GGTATGAAAA TATTAATGAT TTTAATAACA TAAATTTTAA AAGATGCCAA 
GATAAACGAA GAGATCATAG TGATCCTGAC AAAAAGAAAA ACAATAATGA AAATTTAGAG 
ATCATCACGG CTAGTCTCAA AATATCAGTT GCTGAACAAA GTCACATAAG TCTCAAAGAG 
CCTATAAAAG AACAAGAACC TATAACGTCA TACAAACAAT TTTCTAGATC TACCCTTCCA 
ACCAGTATAT CAGATGTTGG TGTCAATACA TTGAGAACAG ATGGCGCCAA GCTTGAGAAT 
AAAATGAAAA TACTTACATC TAACATCAAT AAACAGGATA TTCCCATAAA AACAACTTAC 
AAACGACAGA ACCTAATTTC GAGCTCAAGA CGAATAAGCC AAGAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245416.1
CDS	521..4291
Translation	MEFPKENEESVNCLRSPMAAVKKRSGPLDIALDEANDVSKIWSLPLGNASSMRKPMIFASEHSEGVASPNKVRRRSTKDKEKKRERWLLTRKTWRYMTDAGRKLIPDGYQTGSGNHDLIEDQFQRVCLSEPSFILWSRRTSYPGAFSCSKRRLKPLLRHASASRRKQTVDATKDYHIADRVIELLQTYLKLRDAYKTTTLLGTKTVRPDQTSSPSAQRPNFKNNGDNHQVISSGTSIQTELLSLLKLLSNCPLFTHEGIQLKFGDAPLSILEDKVLLKKIYSALKKQQLHRTLHSKDPYQANFSTSLSCLIKNGKELSRGNTDSNRSNKLLHDNFFCLMKCDTKPIIPSPKTEFFLDLNKVPQNFELKNKLNSNGQSVERIQKTCGTQTNFIQLSELKTLAEQYNLMVTNCDNTLKLLEEPSKQDCSKSSHLIFRRSSLDEDISQSVSDTIKRYLNMARKKSMQDSDSNRFKSINYDKNLRNIKAKGVLNPPGISAGLHKAVQTLNAWPLIALDFIRGNESSLNLKNAHLEWLRLEDERSHMQLERDKNPIELRSKVNPLQIASPANTSSYSKCTSAPTSPTSHSKLEKAIRTSSGLLSSSSQFISNILHGHNNAGNQFNTLTDKLPDCNQSHATASMQKSKSLSNVGQFVAKRMWRSRSKSQNKRSYLKSSIPTLKWYPSEHFLWISESGESFQIVETNLTRLSKIESDIVKNFALEKIQELNIGNIEDLKISPQKRRCVPKKKSLTTSFFDNGKKDDQNPRSVLFGTSLECCLERDSKRNVTMEDRSKYSLLSMFRGSGSTPGSVLKLNDNVRSCESLPSKSLEYGSTELSAYVRGSFSNISKPLASLSQSENEDTELNFVKAYQEHSQLMVPIFVNNCIDYLEDNGLQQVGLFRVSTSKKRVKQLREEFDKDIYFGISVDTCPHDVATLLKEFLRDLPEPLLCNTLYLTFLKTQRIRNRRLQLEAISHLIRLLPIPHRDTLYVLLVFLAKVAAHSDDIWSTEGCCLTLGNKMDSYNLATVFAPNILRSTHLTFSRDKEQENMIDAINVVRTMINHYEEIFNISAELLNVIYTQALEACPEKLYELISTKVYGTEWTQQQIDDPQPGSLSDVLLEPPIHGKRYENINDFNNINFKRCQDKRRDHSDPDKKKNNNENLEIITASLKISVAEQSHISLKEPIKEQEPITSYKQFSRSTLPTSISDVGVNTLRTDGAKLENKMKILTSNINKQDIPIKTTYKRQNLISSSRRISQEP

Target ORF information:

RefSeq Version	NM_001258487.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258487.3

ORF Insert Sequence:

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGAA CCATGATAAA TCACTATGAA 
GAAATTTTCA ACATATCAGC AGAGTTACTG AATGTCATTT ATACACAAGC ACTGGAAGCT 
TGTCCGGAAA AGTTATATGA GCTGATTTCC ACGAAAGTCT ATGGAACTGA ATGGACCCAA 
CAGCAAATAG ACGACCCTCA ACCAGGGAGT TTATCTGATG TTTTGCTCGA ACCTCCGATC 
CACGGAAAGC GGTATGAAAA TATTAATGAT TTTAATAACA TAAATTTTAA AAGATGCCAA 
GATAAACGAA GAGATCATAG TGATCCTGAC AAAAAGAAAA ACAATAATGA AAATTTAGAG 
ATCATCACGG CTAGTCTCAA AATATCAGTT GCTGAACAAA GTCACATAAG TCTCAAAGAG 
CCTATAAAAG AACAAGAACC TATAACGTCA TACAAACAAT TTTCTAGATC TACCCTTCCA 
ACCAGTATAT CAGATGTTGG TGTCAATACA TTGAGAACAG ATGGCGCCAA GCTTGAGAAT 
AAAATGAAAA TACTTACATC TAACATCAAT AAACAGGATA TTCCCATAAA AACAACTTAC 
AAACGACAGA ACCTAATTTC GAGCTCAAGA CGAATAAGCC AAGAACCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP102A ( NM_001272121.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259050.1 Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa00149
Clone ID Related Accession (Same CDS sequence)	NM_001272121.2
Accession Version	NM_001272121.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 102A, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_001272121.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGAA CCATGATAAA TCACTATGAA 
GAAATTTTCA ACATATCAGC AGAGTTACTG AATGTCATTT ATACACAAGC ACTGGAAGCT 
TGTCCGGAAA AGTTATATGA GCTGATTTCC ACGAAAGTCT ATGGAACTGA ATGGACCCAA 
CAGCAAATAG ACGACCCTCA ACCAGGGAGT TTATCTGATG TTTTGCTCGA ACCTCCGATC 
CACGGAAAGC GATGCCAAGA TAAACGAAGA GATCATAGTG ATCCTGACAA AAAGAAAAAC 
AATAATGAAA ATTTAGAGAT CATCACGGCT AGTCTCAAAA TATCAGTTGC TGAACAAAGT 
CACATAAGTC TCAAAGAGCC TATAAAAGAA CAAGAACCTA TAACGTCATA CAAACAATTT 
TCTAGATCTA CCCTTCCAAC CAGTATATCA GATGTTGGTG TCAATACATT GAGAACAGAT 
GGCGCCAAGC TTGAGAATAA AATGAAAATA CTTACATCTA ACATCAATAA ACAGGATATT 
CCCATAAAAA CAACTTACAA ACGACAGAAC CTAATTTCGA GCTCAAGACG AATAAGCCAA 
GAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259050.1
CDS	521..4249
Translation	MEFPKENEESVNCLRSPMAAVKKRSGPLDIALDEANDVSKIWSLPLGNASSMRKPMIFASEHSEGVASPNKVRRRSTKDKEKKRERWLLTRKTWRYMTDAGRKLIPDGYQTGSGNHDLIEDQFQRVCLSEPSFILWSRRTSYPGAFSCSKRRLKPLLRHASASRRKQTVDATKDYHIADRVIELLQTYLKLRDAYKTTTLLGTKTVRPDQTSSPSAQRPNFKNNGDNHQVISSGTSIQTELLSLLKLLSNCPLFTHEGIQLKFGDAPLSILEDKVLLKKIYSALKKQQLHRTLHSKDPYQANFSTSLSCLIKNGKELSRGNTDSNRSNKLLHDNFFCLMKCDTKPIIPSPKTEFFLDLNKVPQNFELKNKLNSNGQSVERIQKTCGTQTNFIQLSELKTLAEQYNLMVTNCDNTLKLLEEPSKQDCSKSSHLIFRRSSLDEDISQSVSDTIKRYLNMARKKSMQDSDSNRFKSINYDKNLRNIKAKGVLNPPGISAGLHKAVQTLNAWPLIALDFIRGNESSLNLKNAHLEWLRLEDERSHMQLERDKNPIELRSKVNPLQIASPANTSSYSKCTSAPTSPTSHSKLEKAIRTSSGLLSSSSQFISNILHGHNNAGNQFNTLTDKLPDCNQSHATASMQKSKSLSNVGQFVAKRMWRSRSKSQNKRSYLKSSIPTLKWYPSEHFLWISESGESFQIVETNLTRLSKIESDIVKNFALEKIQELNIGNIEDLKISPQKRRCVPKKKSLTTSFFDNGKKDDQNPRSVLFGTSLECCLERDSKRNVTMEDRSKYSLLSMFRGSGSTPGSVLKLNDNVRSCESLPSKSLEYGSTELSAYVRGSFSNISKPLASLSQSENEDTELNFVKAYQEHSQLMVPIFVNNCIDYLEDNGLQQVGLFRVSTSKKRVKQLREEFDKDIYFGISVDTCPHDVATLLKEFLRDLPEPLLCNTLYLTFLKTQRIRNRRLQLEAISHLIRLLPIPHRDTLYVLLVFLAKVAAHSDDIWSTEGCCLTLGNKMDSYNLATVFAPNILRSTHLTFSRDKEQENMIDAINVVRTMINHYEEIFNISAELLNVIYTQALEACPEKLYELISTKVYGTEWTQQQIDDPQPGSLSDVLLEPPIHGKRCQDKRRDHSDPDKKKNNNENLEIITASLKISVAEQSHISLKEPIKEQEPITSYKQFSRSTLPTSISDVGVNTLRTDGAKLENKMKILTSNINKQDIPIKTTYKRQNLISSSRRISQEP

Target ORF information:

RefSeq Version	NM_001272121.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272121.2

ORF Insert Sequence:

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGAA CCATGATAAA TCACTATGAA 
GAAATTTTCA ACATATCAGC AGAGTTACTG AATGTCATTT ATACACAAGC ACTGGAAGCT 
TGTCCGGAAA AGTTATATGA GCTGATTTCC ACGAAAGTCT ATGGAACTGA ATGGACCCAA 
CAGCAAATAG ACGACCCTCA ACCAGGGAGT TTATCTGATG TTTTGCTCGA ACCTCCGATC 
CACGGAAAGC GATGCCAAGA TAAACGAAGA GATCATAGTG ATCCTGACAA AAAGAAAAAC 
AATAATGAAA ATTTAGAGAT CATCACGGCT AGTCTCAAAA TATCAGTTGC TGAACAAAGT 
CACATAAGTC TCAAAGAGCC TATAAAAGAA CAAGAACCTA TAACGTCATA CAAACAATTT 
TCTAGATCTA CCCTTCCAAC CAGTATATCA GATGTTGGTG TCAATACATT GAGAACAGAT 
GGCGCCAAGC TTGAGAATAA AATGAAAATA CTTACATCTA ACATCAATAA ACAGGATATT 
CCCATAAAAA CAACTTACAA ACGACAGAAC CTAATTTCGA GCTCAAGACG AATAAGCCAA 
GAACCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP102A ( NM_143657.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651914.3 Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant E, mRNA.

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CloneID	OFa00249
Clone ID Related Accession (Same CDS sequence)	NM_143657.5
Accession Version	NM_143657.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 102A, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_143657.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGTC GCAGAGGTTC TAAGGGAACT 
GAGGAACCCC AACCCAAAAG GAAGTGGATA AGTCGTCCAG GACCAGATGC CCAACAAAAA 
AGTCCTGTCA TGCTTTTACC GCATGCGTCA TATAAAAAAT ATAGTGATAT ATTGTCATTG 
CGGCTGCGTC AGGAACTCAA ATTGGAAAGA ACAAATCTAG AGCGAAGAGC TTTTATACAC 
ATAAATAACA AGGAATGCAA TGGTCTAGGA TCTCGACCAG CAGATACCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651914.3
CDS	521..3934
Translation	MEFPKENEESVNCLRSPMAAVKKRSGPLDIALDEANDVSKIWSLPLGNASSMRKPMIFASEHSEGVASPNKVRRRSTKDKEKKRERWLLTRKTWRYMTDAGRKLIPDGYQTGSGNHDLIEDQFQRVCLSEPSFILWSRRTSYPGAFSCSKRRLKPLLRHASASRRKQTVDATKDYHIADRVIELLQTYLKLRDAYKTTTLLGTKTVRPDQTSSPSAQRPNFKNNGDNHQVISSGTSIQTELLSLLKLLSNCPLFTHEGIQLKFGDAPLSILEDKVLLKKIYSALKKQQLHRTLHSKDPYQANFSTSLSCLIKNGKELSRGNTDSNRSNKLLHDNFFCLMKCDTKPIIPSPKTEFFLDLNKVPQNFELKNKLNSNGQSVERIQKTCGTQTNFIQLSELKTLAEQYNLMVTNCDNTLKLLEEPSKQDCSKSSHLIFRRSSLDEDISQSVSDTIKRYLNMARKKSMQDSDSNRFKSINYDKNLRNIKAKGVLNPPGISAGLHKAVQTLNAWPLIALDFIRGNESSLNLKNAHLEWLRLEDERSHMQLERDKNPIELRSKVNPLQIASPANTSSYSKCTSAPTSPTSHSKLEKAIRTSSGLLSSSSQFISNILHGHNNAGNQFNTLTDKLPDCNQSHATASMQKSKSLSNVGQFVAKRMWRSRSKSQNKRSYLKSSIPTLKWYPSEHFLWISESGESFQIVETNLTRLSKIESDIVKNFALEKIQELNIGNIEDLKISPQKRRCVPKKKSLTTSFFDNGKKDDQNPRSVLFGTSLECCLERDSKRNVTMEDRSKYSLLSMFRGSGSTPGSVLKLNDNVRSCESLPSKSLEYGSTELSAYVRGSFSNISKPLASLSQSENEDTELNFVKAYQEHSQLMVPIFVNNCIDYLEDNGLQQVGLFRVSTSKKRVKQLREEFDKDIYFGISVDTCPHDVATLLKEFLRDLPEPLLCNTLYLTFLKTQRIRNRRLQLEAISHLIRLLPIPHRDTLYVLLVFLAKVAAHSDDIWSTEGCCLTLGNKMDSYNLATVFAPNILRSTHLTFSRDKEQENMIDAINVVSRRGSKGTEEPQPKRKWISRPGPDAQQKSPVMLLPHASYKKYSDILSLRLRQELKLERTNLERRAFIHINNKECNGLGSRPADTR

Target ORF information:

RefSeq Version	NM_143657.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143657.5

ORF Insert Sequence:

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGTC GCAGAGGTTC TAAGGGAACT 
GAGGAACCCC AACCCAAAAG GAAGTGGATA AGTCGTCCAG GACCAGATGC CCAACAAAAA 
AGTCCTGTCA TGCTTTTACC GCATGCGTCA TATAAAAAAT ATAGTGATAT ATTGTCATTG 
CGGCTGCGTC AGGAACTCAA ATTGGAAAGA ACAAATCTAG AGCGAAGAGC TTTTATACAC 
ATAAATAACA AGGAATGCAA TGGTCTAGGA TCTCGACCAG CAGATACCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RhoGAP102A ( NM_001038713.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001033802.2 Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant C, mRNA.

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CloneID	OFa00250
Clone ID Related Accession (Same CDS sequence)	NM_001038713.4
Accession Version	NM_001038713.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rho GTPase activating protein at 102A, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jul 15, 2014 this sequence version replaced NM_001038713.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGGT TTGTCAAGCA AAATACATAT 
TTGCTTTTTA TGCCTTTGAT AAGACCTAAT CTACCGTCTC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033802.2
CDS	521..3745
Translation	MEFPKENEESVNCLRSPMAAVKKRSGPLDIALDEANDVSKIWSLPLGNASSMRKPMIFASEHSEGVASPNKVRRRSTKDKEKKRERWLLTRKTWRYMTDAGRKLIPDGYQTGSGNHDLIEDQFQRVCLSEPSFILWSRRTSYPGAFSCSKRRLKPLLRHASASRRKQTVDATKDYHIADRVIELLQTYLKLRDAYKTTTLLGTKTVRPDQTSSPSAQRPNFKNNGDNHQVISSGTSIQTELLSLLKLLSNCPLFTHEGIQLKFGDAPLSILEDKVLLKKIYSALKKQQLHRTLHSKDPYQANFSTSLSCLIKNGKELSRGNTDSNRSNKLLHDNFFCLMKCDTKPIIPSPKTEFFLDLNKVPQNFELKNKLNSNGQSVERIQKTCGTQTNFIQLSELKTLAEQYNLMVTNCDNTLKLLEEPSKQDCSKSSHLIFRRSSLDEDISQSVSDTIKRYLNMARKKSMQDSDSNRFKSINYDKNLRNIKAKGVLNPPGISAGLHKAVQTLNAWPLIALDFIRGNESSLNLKNAHLEWLRLEDERSHMQLERDKNPIELRSKVNPLQIASPANTSSYSKCTSAPTSPTSHSKLEKAIRTSSGLLSSSSQFISNILHGHNNAGNQFNTLTDKLPDCNQSHATASMQKSKSLSNVGQFVAKRMWRSRSKSQNKRSYLKSSIPTLKWYPSEHFLWISESGESFQIVETNLTRLSKIESDIVKNFALEKIQELNIGNIEDLKISPQKRRCVPKKKSLTTSFFDNGKKDDQNPRSVLFGTSLECCLERDSKRNVTMEDRSKYSLLSMFRGSGSTPGSVLKLNDNVRSCESLPSKSLEYGSTELSAYVRGSFSNISKPLASLSQSENEDTELNFVKAYQEHSQLMVPIFVNNCIDYLEDNGLQQVGLFRVSTSKKRVKQLREEFDKDIYFGISVDTCPHDVATLLKEFLRDLPEPLLCNTLYLTFLKTQRIRNRRLQLEAISHLIRLLPIPHRDTLYVLLVFLAKVAAHSDDIWSTEGCCLTLGNKMDSYNLATVFAPNILRSTHLTFSRDKEQENMIDAINVVRFVKQNTYLLFMPLIRPNLPSR

Target ORF information:

RefSeq Version	NM_001038713.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rho GTPase activating protein at 102A (RhoGAP102A), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001038713.4

ORF Insert Sequence:

ATGGAATTTC CAAAAGAAAA CGAAGAATCT GTGAACTGTT TGAGGAGCCC CATGGCGGCC 
GTTAAAAAAA GGTCGGGGCC GTTAGATATA GCACTTGACG AGGCTAACGA CGTATCGAAA 
ATATGGTCGT TGCCATTAGG AAATGCCTCC AGCATGCGTA AGCCAATGAT TTTTGCGAGT 
GAGCACTCCG AAGGAGTGGC TTCTCCAAAT AAAGTGCGTC GTCGCAGCAC AAAAGACAAG 
GAAAAAAAGC GAGAGCGCTG GCTTCTAACT CGAAAAACCT GGAGATATAT GACAGATGCA 
GGCCGTAAGC TAATTCCTGA TGGATACCAA ACAGGGTCAG GTAATCATGA CCTAATTGAA 
GATCAATTTC AACGGGTGTG TTTATCTGAA CCAAGTTTTA TTTTATGGAG TCGCAGAACA 
TCATACCCAG GAGCATTCAG CTGTTCTAAG CGGAGATTAA AACCATTGTT GAGGCATGCA 
AGTGCTAGCC GCAGAAAACA GACAGTTGAT GCAACCAAAG ATTATCATAT TGCCGATCGA 
GTCATCGAAT TATTGCAGAC TTATCTTAAA CTTCGTGATG CATACAAAAC CACAACTTTA 
CTTGGAACTA AAACAGTTCG GCCAGATCAA ACATCATCAC CCAGTGCACA AAGGCCAAAC 
TTCAAAAACA ATGGTGACAA CCATCAAGTG ATATCATCCG GCACATCTAT TCAAACAGAA 
CTCTTGTCCC TTCTTAAATT GTTATCTAAT TGCCCTTTAT TTACACATGA AGGAATTCAG 
CTTAAGTTTG GCGATGCTCC ACTAAGCATT CTCGAAGACA AAGTTCTTTT GAAAAAAATC 
TATAGCGCGC TTAAGAAACA ACAGCTCCAT AGGACGCTAC ATTCCAAAGA TCCATATCAA 
GCTAATTTTT CGACTTCATT GTCTTGCTTG ATAAAAAATG GAAAGGAATT ATCTAGAGGT 
AACACTGATA GTAACAGAAG CAACAAGTTA CTACATGACA ATTTCTTTTG TCTAATGAAA 
TGTGATACCA AACCAATTAT ACCAAGCCCC AAAACAGAAT TCTTTTTAGA CCTCAATAAA 
GTCCCGCAGA ACTTTGAGCT TAAAAACAAA CTTAATTCGA ATGGACAGAG CGTTGAAAGA 
ATCCAGAAAA CCTGTGGAAC ACAAACTAAT TTTATACAAC TGAGCGAGTT AAAAACTTTG 
GCGGAGCAGT ACAACTTAAT GGTTACAAAT TGCGATAATA CCTTAAAATT ATTAGAAGAG 
CCTTCTAAAC AAGACTGTTC AAAGTCTTCG CATTTAATAT TTAGAAGAAG TTCACTTGAC 
GAAGACATTT CTCAGTCAGT TAGCGATACG ATTAAACGGT ATTTGAACAT GGCAAGAAAA 
AAAAGTATGC AAGATTCTGA TTCAAACCGA TTTAAATCAA TTAACTATGA TAAGAACTTG 
AGAAATATCA AAGCAAAGGG CGTACTAAAT CCACCGGGAA TAAGTGCAGG ATTGCACAAG 
GCTGTACAAA CGTTAAATGC TTGGCCTCTC ATAGCTTTGG ATTTTATACG AGGAAACGAA 
TCGTCTTTGA ATCTTAAAAA CGCTCACTTA GAATGGTTAC GATTAGAGGA TGAGCGAAGT 
CACATGCAGT TAGAAAGAGA TAAAAATCCA ATAGAATTAA GGAGTAAAGT GAATCCTTTA 
CAAATCGCAA GTCCTGCAAA TACCTCTTCA TATTCCAAGT GCACTTCTGC ACCAACTTCG 
CCGACTAGTC ATTCAAAGTT AGAAAAGGCT ATACGGACTT CCAGTGGATT ACTCTCTTCA 
AGTTCTCAAT TTATTTCAAA TATTTTGCAC GGACATAACA ATGCTGGAAA CCAATTTAAC 
ACATTAACAG ACAAACTGCC AGATTGCAAT CAGAGTCATG CAACGGCAAG TATGCAGAAG 
TCAAAGTCGT TATCTAATGT GGGCCAATTT GTAGCAAAAC GAATGTGGCG GAGTCGATCT 
AAAAGCCAAA ATAAACGGAG TTACCTAAAG AGCTCTATAC CAACTCTAAA ATGGTATCCG 
TCGGAACATT TTTTATGGAT ATCAGAGTCT GGTGAATCTT TTCAAATAGT GGAGACAAAT 
TTGACAAGGC TGTCAAAAAT AGAATCGGAT ATAGTAAAAA ATTTTGCATT AGAAAAAATT 
CAGGAACTAA ACATTGGAAA TATTGAAGAT CTGAAGATAT CACCACAAAA ACGACGATGT 
GTACCTAAAA AAAAATCATT GACTACAAGC TTCTTTGATA ATGGAAAAAA AGATGATCAG 
AATCCTCGCT CAGTATTGTT CGGAACTTCT TTGGAATGTT GCTTAGAAAG AGACAGCAAA 
CGGAACGTTA CTATGGAAGA CCGATCTAAG TATTCACTTC TGTCAATGTT TCGTGGAAGC 
GGTAGTACGC CAGGAAGTGT TTTGAAGCTT AATGATAATG TCAGGTCATG TGAAAGCTTA 
CCATCAAAAT CCCTTGAATA TGGAAGCACG GAACTTTCTG CATATGTTAG AGGCTCCTTT 
TCCAATATAT CAAAGCCTTT AGCATCGCTA TCTCAGTCTG AAAATGAAGA CACTGAACTT 
AATTTCGTTA AAGCCTATCA AGAGCATTCT CAATTAATGG TACCAATATT CGTAAACAAC 
TGTATTGATT ATTTAGAAGA CAACGGGTTA CAACAGGTTG GACTCTTTCG GGTGAGCACA 
TCCAAAAAAA GAGTAAAGCA ATTGCGGGAG GAATTCGATA AAGATATTTA TTTTGGGATT 
TCTGTTGATA CATGCCCACA TGATGTTGCC ACACTGCTTA AAGAATTTTT ACGTGATCTT 
CCGGAACCAT TACTTTGCAA CACACTCTAT TTAACGTTTT TAAAAACACA ACGTATTCGG 
AATCGACGCT TGCAACTAGA GGCTATATCA CATTTGATAA GACTACTTCC GATCCCACAC 
AGAGACACGT TGTACGTCTT ACTAGTATTT CTAGCCAAAG TGGCAGCACA CAGCGATGAT 
ATATGGTCTA CAGAGGGATG TTGTTTAACG CTTGGAAATA AAATGGATAG CTATAATCTA 
GCTACAGTAT TTGCTCCAAA TATTTTGCGA AGCACTCATT TGACATTTTC GAGAGATAAG 
GAACAAGAAA ATATGATCGA TGCCATTAAT GTAGTAAGGT TTGTCAAGCA AAATACATAT 
TTGCTTTTTA TGCCTTTGAT AAGACCTAAT CTACCGTCTC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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RhoGAP102A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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