spn-F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spn-F
Entrez Gene ID	43700
Full Name	CG12114 gene product from transcript CG12114-RA
Synonyms	1130/03,CG12114,Dmel\CG12114,Spn-F,SpnF,l(3)S113003b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100B8-100B9\|3-100 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_143601.3	NP_651858.1	spn-F, isoform A
NM_001276209.1	NP_001263138.1	spn-F, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	spn-F	NP_001263138.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000758	XP_311294.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons
Title: Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.

These results suggest that polarized transport and selective retention regulate the distal tip localization of the Spn-F-IKKepsilon complex during bristle cell elongation.
Title: A transport and retention mechanism for the sustained distal localization of Spn-F-IKK? during Drosophila bristle elongation.

Spn-F mediates the interaction between Ik2 and Jvl to control Ik2 activity.
Title: Drosophila oocyte polarity and cytoskeleton organization require regulation of Ik2 activity by Spn-F and Javelin-like.

Spn-F and Ik2 are localized to the bristle tip and that mutations in ik2 and spn-F affect bristle microtubules and actin organization
Title: Asymmetric microtubule function is an essential requirement for polarized organization of the Drosophila bristle.

IkappaB kinase 2 binds physically to Spindle-F protein, biomolecular interaction analysis of Spindle-F protein and IkappaB kinase 2 demonstrating that both proteins bind directly and form a complex
Title: The Drosophila IKK-related kinase (Ik2) and Spindle-F proteins are part of a complex that regulates cytoskeleton organization during oogenesis.

The following spn-F gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spn-F cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06751	NM_143601.3 Latest version!	Drosophila melanogaster spn-F, transcript variant A (spn-F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07867	NM_001276209.1 Latest version!	Drosophila melanogaster spn-F, transcript variant B (spn-F), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spn-F ( NM_143601.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651858.1 Drosophila melanogaster spn-F, transcript variant A (spn-F), mRNA.

Price & Availability↑

CloneID	OFa06751
Clone ID Related Accession (Same CDS sequence)	NM_143601.3
Accession Version	NM_143601.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spn-F, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143601.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCAT CTGCTGCCAA AATCACGCCT ATGGCCAGTT CCATGTCCGC TTCCGGATCC 
ACAAACTCCC CCTCCAGTGA AAAAATGAAC TACGCACTGC AAGTGGCGCT GCAGACGATC 
AAGGAGCGGT GCATCCAGCT GCAGCGCCGC GTGGCCAGCA TGGAGGAGGA GAACCAGCAG 
CTAAGGGAGG CCTCCAGCAG GTCGGAAGGT GCTCCAAGGG CAAACGAAAT CGGGGTCACT 
GGGGACGTCC TCTCTCTGAA AGCACAGGTC TCTGAGCTGC AGCGCCAAAA GGAGCAGCTG 
GAGGAGCACA TTGGCATGGT ATCCAACGAG AACAGACGGC TCTGGTCCCG CCTATCGCAG 
ATCTCCAAGG ATCAGCAGCT GAACGCACTG CCCAGCTCAA CAGACTCGCG AGCCCAGCAA 
AACCAGAACC TGGTGCGCTC CAAGACCTTT ACGCAGCACT CCCCCAATCC TCACCTTCGC 
CAAAAGATGC TCTCCGACGG CATTAAGGAT CTCAGTCTGG AGGAAATAGC CCTGGATGAC 
TTCGGTGCCA GCAGTGAGGA ACTAGGTTAC CCCTACAACC TGCAAAAGGT GGAGGAAACC 
ACTAGTGAAC CAGATGCCAA TGTGGATGCC AAAAGATGTT TGGATGGACT GCAGGAGCTG 
AGGCGAGAGG CCATGAAGCA GCAGCAGGAG CTCAGATCGG TAATGACTCT GCTAGAAAAT 
CGCATAGCAC TCAAGCCCTG TCCGGAATGT GCCCAGAAAA CCATCAAAAA ACCGGAGATG 
GCCGACAAAA GTCTGGAAAC GGACGACAGC CTGACCAGCG AGCTGAAGAA CTATGAGAGC 
CAACACAATG GACACAATGG CACGCCGCCC AGCCAGAGAA TTAACATAAT ACAAGAGAAA 
ATCAAAGCAG ATGCAGCCGA CGCAATGGAG AAGACCTGCC CTATGTGCGG CAAGCAGTAC 
TCCAGCCAAG TGTCCTTCAA TGCGTTCCGC GAGCACGTCG AGATGCACTT CATCGACGAT 
GCACTGGAAC TGGAGTCGGA GAACAGCATT GAGCGCCAGT TTGAGTTCGT TTCCCATGCT 
GTGGGTGACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651858.1
CDS	117..1211
Translation	MEASAAKITPMASSMSASGSTNSPSSEKMNYALQVALQTIKERCIQLQRRVASMEEENQQLREASSRSEGAPRANEIGVTGDVLSLKAQVSELQRQKEQLEEHIGMVSNENRRLWSRLSQISKDQQLNALPSSTDSRAQQNQNLVRSKTFTQHSPNPHLRQKMLSDGIKDLSLEEIALDDFGASSEELGYPYNLQKVEETTSEPDANVDAKRCLDGLQELRREAMKQQQELRSVMTLLENRIALKPCPECAQKTIKKPEMADKSLETDDSLTSELKNYESQHNGHNGTPPSQRINIIQEKIKADAADAMEKTCPMCGKQYSSQVSFNAFREHVEMHFIDDALELESENSIERQFEFVSHAVGDF

Target ORF information:

RefSeq Version	NM_143601.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spn-F, transcript variant A (spn-F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143601.3

ORF Insert Sequence:

ATGGAGGCAT CTGCTGCCAA AATCACGCCT ATGGCCAGTT CCATGTCCGC TTCCGGATCC 
ACAAACTCCC CCTCCAGTGA AAAAATGAAC TACGCACTGC AAGTGGCGCT GCAGACGATC 
AAGGAGCGGT GCATCCAGCT GCAGCGCCGC GTGGCCAGCA TGGAGGAGGA GAACCAGCAG 
CTAAGGGAGG CCTCCAGCAG GTCGGAAGGT GCTCCAAGGG CAAACGAAAT CGGGGTCACT 
GGGGACGTCC TCTCTCTGAA AGCACAGGTC TCTGAGCTGC AGCGCCAAAA GGAGCAGCTG 
GAGGAGCACA TTGGCATGGT ATCCAACGAG AACAGACGGC TCTGGTCCCG CCTATCGCAG 
ATCTCCAAGG ATCAGCAGCT GAACGCACTG CCCAGCTCAA CAGACTCGCG AGCCCAGCAA 
AACCAGAACC TGGTGCGCTC CAAGACCTTT ACGCAGCACT CCCCCAATCC TCACCTTCGC 
CAAAAGATGC TCTCCGACGG CATTAAGGAT CTCAGTCTGG AGGAAATAGC CCTGGATGAC 
TTCGGTGCCA GCAGTGAGGA ACTAGGTTAC CCCTACAACC TGCAAAAGGT GGAGGAAACC 
ACTAGTGAAC CAGATGCCAA TGTGGATGCC AAAAGATGTT TGGATGGACT GCAGGAGCTG 
AGGCGAGAGG CCATGAAGCA GCAGCAGGAG CTCAGATCGG TAATGACTCT GCTAGAAAAT 
CGCATAGCAC TCAAGCCCTG TCCGGAATGT GCCCAGAAAA CCATCAAAAA ACCGGAGATG 
GCCGACAAAA GTCTGGAAAC GGACGACAGC CTGACCAGCG AGCTGAAGAA CTATGAGAGC 
CAACACAATG GACACAATGG CACGCCGCCC AGCCAGAGAA TTAACATAAT ACAAGAGAAA 
ATCAAAGCAG ATGCAGCCGA CGCAATGGAG AAGACCTGCC CTATGTGCGG CAAGCAGTAC 
TCCAGCCAAG TGTCCTTCAA TGCGTTCCGC GAGCACGTCG AGATGCACTT CATCGACGAT 
GCACTGGAAC TGGAGTCGGA GAACAGCATT GAGCGCCAGT TTGAGTTCGT TTCCCATGCT 
GTGGGTGACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spn-F ( NM_001276209.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263138.1 Drosophila melanogaster spn-F, transcript variant B (spn-F), mRNA.

Price & Availability↑

CloneID	OFa07867
Clone ID Related Accession (Same CDS sequence)	NM_001276209.1
Accession Version	NM_001276209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	spn-F, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGCAT CTGCTGCCAA AATCACGCCT ATGGCCAGTT CCATGTCCGC TTCCGGATCC 
ACAAACTCCC CCTCCAGTGA AAAAATGAAC TACGCACTGC AAGTGGCGCT GCAGACGATC 
AAGGAGCGGT GCATCCAGCT GCAGCGCCGC GTGGCCAGCA TGGAGGAGGA GAACCAGCAG 
CTAAGGGAGG CCTCCAGCAG GTCGGAAGGT GCTCCAAGGG CAAACGAAAT CGGGGTCACT 
GGGGACGTCC TCTCTCTGAA AGCACAGGTC TCTGAGCTGC AGCGCCAAAA GGAGCAGCTG 
GAGGAGCACA TTGGCATGGT ATCCAACGAG AACAGACGGC TCTGGTCCCG CCTATCGCAG 
ATCTCCAAGG ATCAGCAGCT GAACGCACTG CCCAGCTCAA CAGACTCGCG AGCCCAGCAA 
AACCAGAACC TGGTGCGCTC CAAGACCTTT ACGCAGCACT CCCCCAATCC TCACCTTCGC 
CAAAAGATGC TCTCCGACGG CATTAAGGAT CTCAGTCTGG AGGAAATAGC CCTGGATGAC 
TTCGGTGCCA GCAGTGAGGA ACTAGGTTAC CCCTACAACC TGCAAAAGGT GGAGGAAACC 
ACTAGTGAAC CAGATGCCAA TGTGGATGCC AAAAGATGTT TGGATGGACT GCAGGAGCTG 
AGGCGAGAGG CCATGAAGCA GCAGCAGGAG CTCAGATCGG TAATGACTCT GCTAGAAAAT 
CGCATAGCAC TCAAGCCCTG TCCGGAATGT GCCCAGAAAA CCATCAAAAA ACCGGAGATG 
GCCGACAAAA GTCTGGAAAC GGACGACAGC CTGACCAGCG AGCTGAAGAA CTATGAGAGC 
CAACACAATG GACACAATGG CACGCCGCCC AGCCAGAGAA TTAACATAAT ACAAGAGAAA 
ATCAAAGCAG ATGCAGCCGA CGCAATGGAG AAGACCTGCC CTATGTGCGG CAAGCAGTAC 
TCCAGCCAAG TGTCCTTCAA TGCGTTCCGC GAGCACGTCG AGATGCACTT CATCGACGAT 
GCACTGGAAC TGGAGTCGGA GAACAGCATT GAGCGCCAGT TTGAGTTCGT TTCCCATGCT 
GTGGGTGACT TCTGACCCAG TAGGCGCATT TATTTCGCCA CCGAACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263138.1
CDS	117..1247
Translation	MEASAAKITPMASSMSASGSTNSPSSEKMNYALQVALQTIKERCIQLQRRVASMEEENQQLREASSRSEGAPRANEIGVTGDVLSLKAQVSELQRQKEQLEEHIGMVSNENRRLWSRLSQISKDQQLNALPSSTDSRAQQNQNLVRSKTFTQHSPNPHLRQKMLSDGIKDLSLEEIALDDFGASSEELGYPYNLQKVEETTSEPDANVDAKRCLDGLQELRREAMKQQQELRSVMTLLENRIALKPCPECAQKTIKKPEMADKSLETDDSLTSELKNYESQHNGHNGTPPSQRINIIQEKIKADAADAMEKTCPMCGKQYSSQVSFNAFREHVEMHFIDDALELESENSIERQFEFVSHAVGDF*PSRRIYFATEL

Target ORF information:

RefSeq Version	NM_001276209.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster spn-F, transcript variant B (spn-F), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276209.1

ORF Insert Sequence:

ATGGAGGCAT CTGCTGCCAA AATCACGCCT ATGGCCAGTT CCATGTCCGC TTCCGGATCC 
ACAAACTCCC CCTCCAGTGA AAAAATGAAC TACGCACTGC AAGTGGCGCT GCAGACGATC 
AAGGAGCGGT GCATCCAGCT GCAGCGCCGC GTGGCCAGCA TGGAGGAGGA GAACCAGCAG 
CTAAGGGAGG CCTCCAGCAG GTCGGAAGGT GCTCCAAGGG CAAACGAAAT CGGGGTCACT 
GGGGACGTCC TCTCTCTGAA AGCACAGGTC TCTGAGCTGC AGCGCCAAAA GGAGCAGCTG 
GAGGAGCACA TTGGCATGGT ATCCAACGAG AACAGACGGC TCTGGTCCCG CCTATCGCAG 
ATCTCCAAGG ATCAGCAGCT GAACGCACTG CCCAGCTCAA CAGACTCGCG AGCCCAGCAA 
AACCAGAACC TGGTGCGCTC CAAGACCTTT ACGCAGCACT CCCCCAATCC TCACCTTCGC 
CAAAAGATGC TCTCCGACGG CATTAAGGAT CTCAGTCTGG AGGAAATAGC CCTGGATGAC 
TTCGGTGCCA GCAGTGAGGA ACTAGGTTAC CCCTACAACC TGCAAAAGGT GGAGGAAACC 
ACTAGTGAAC CAGATGCCAA TGTGGATGCC AAAAGATGTT TGGATGGACT GCAGGAGCTG 
AGGCGAGAGG CCATGAAGCA GCAGCAGGAG CTCAGATCGG TAATGACTCT GCTAGAAAAT 
CGCATAGCAC TCAAGCCCTG TCCGGAATGT GCCCAGAAAA CCATCAAAAA ACCGGAGATG 
GCCGACAAAA GTCTGGAAAC GGACGACAGC CTGACCAGCG AGCTGAAGAA CTATGAGAGC 
CAACACAATG GACACAATGG CACGCCGCCC AGCCAGAGAA TTAACATAAT ACAAGAGAAA 
ATCAAAGCAG ATGCAGCCGA CGCAATGGAG AAGACCTGCC CTATGTGCGG CAAGCAGTAC 
TCCAGCCAAG TGTCCTTCAA TGCGTTCCGC GAGCACGTCG AGATGCACTT CATCGACGAT 
GCACTGGAAC TGGAGTCGGA GAACAGCATT GAGCGCCAGT TTGAGTTCGT TTCCCATGCT 
GTGGGTGACT TCTGACCCAG TAGGCGCATT TATTTCGCCA CCGAACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spn-F cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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