dj-1beta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dj-1beta
Entrez Gene ID	43652
Full Name	CG1349 gene product from transcript CG1349-RB
Synonyms	CG1349,DJ-1,DJ-1B,DJ-1b,DJ-1beta,Dmel\CG1349
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 100A5-100A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_143568.3	NP_651825.4	dj-1beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	PARK7	NP_009193.2
Canis lupus familiaris (dog)	PARK7	XP_859031.1
Mus musculus (house mouse)	Park7	NP_065594.2
Gallus gallus (chicken)	PARK7	NP_989916.1
Danio rerio (zebrafish)	park7	NP_001005938.1
Caenorhabditis elegans (roundworm)	djr-1.2	NP_504132.1
Oryza sativa (rice)	Os06g0531200	NP_001057783.2
Bos taurus (cattle)	PARK7	NP_001015572.1
Drosophila melanogaster (fruit fly)	DJ-1alpha	NP_610916.1
Caenorhabditis elegans (roundworm)	djr-1.1	NP_493696.1
Oryza sativa (rice)	Os01g0217800	NP_001042407.2
Drosophila melanogaster (fruit fly)	dj-1beta	NP_651825.3
Arabidopsis thaliana (thale cress)	AT1G53280	NP_564626.1
Rattus norvegicus (Norway rat)	Park7	NP_001264180.1
Schizosaccharomyces pombe (fission yeast)	SPAC22E12.03c	NP_594829.1
Arabidopsis thaliana (thale cress)	AT3G14990	NP_188117.1
Xenopus tropicalis (tropical clawed frog)	park7	NP_001015851.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dj-1beta regulates oxidative stress, insulin-like signaling and development in Drosophila melanogaster.
Title: dj-1? regulates oxidative stress, insulin-like signaling and development in Drosophila melanogaster.

the oxidation of conserved cysteine residue to its sulfinate form (Cys-SO(2)(-)) results in considerable thermal stabilization of both Drosophila DJ-1beta and human DJ-1
Title: Conservation of oxidative protein stabilization in an insect homologue of parkinsonism-associated protein DJ-1.

Flies deleted for DJ-1 manifest similar defects as pink1 and parkin mutants: male sterility, shortened lifespan, and reduced climbing ability.
Title: DJ-1 is critical for mitochondrial function and rescues PINK1 loss of function.

Data show that human DJ-1 rescues flies mutant for DJ-1b; a conserved cysteine in is critical for antioxidant function, and suggest that overoxidation of DJ-1 with age and exposure to oxidants can cause inactivation.
Title: Mutational analysis of DJ-1 in Drosophila implicates functional inactivation by oxidative damage and aging.

Loss-of-function DJ-1beta mutants demonstrated an extended survival of dopaminergic neurons and resistance to paraquat stress, but showed acute sensitivity to hydrogen peroxide treatment.
Title: Roles of Drosophila DJ-1 in survival of dopaminergic neurons and oxidative stress.

The following dj-1beta gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dj-1beta cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa41217	NM_143568.3 Latest version!	Drosophila melanogaster dj-1beta (dj-1beta), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dj-1beta ( NM_143568.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651825.4 Drosophila melanogaster dj-1beta (dj-1beta), mRNA.

Price & Availability↑

CloneID	OFa41217
Clone ID Related Accession (Same CDS sequence)	NM_143568.3
Accession Version	NM_143568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dj-1beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143568.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGAAAA GCGCGCTGGT GATTTTGGCT CCCGGAGCGG AGGAGATGGA GTTCATCATA 
GCCGCCGACG TTCTGAGAAG GGCGGGCATC AAGGTCACCG TAGCCGGTTT GAATGGCGGG 
GAAGCGGTGA AGTGCTCGCG GGATGTGCAG ATCCTGCCGG ACACCTCGCT GGCCCAGGTT 
GCCTCGGATA AGTTCGATGT GGTGGTGCTG CCCGGCGGAC TGGGTGGCTC CAATGCCATG 
GGGGAGTCCT CGCTGGTCGG TGACTTGCTG CGCAGCCAGG AGTCTGGTGG CGGACTCATC 
GCCGCCATCT GTGCCGCGCC CACCGTTTTG GCCAAGCACG GCGTCGCCTC CGGGAAATCC 
CTCACCTCGT ATCCCTCAAT GAAGCCCCAG CTGGTGAATA ACTATAGCTA TGTGGACGAC 
AAGACGGTGG TCAAGGATGG CAATCTGATC ACCAGTCGAG GTCCTGGCAC CGCCTACGAG 
TTCGCCCTCA AAATCGCCGA GGAGCTGGCG GGCAAGGAGA AAGTCCAGGA GGTGGCCAAG 
GGTCTTCTTG TGGCCTACAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651825.4
CDS	82..645
Translation	MSKSALVILAPGAEEMEFIIAADVLRRAGIKVTVAGLNGGEAVKCSRDVQILPDTSLAQVASDKFDVVVLPGGLGGSNAMGESSLVGDLLRSQESGGGLIAAICAAPTVLAKHGVASGKSLTSYPSMKPQLVNNYSYVDDKTVVKDGNLITSRGPGTAYEFALKIAEELAGKEKVQEVAKGLLVAYN

Target ORF information:

RefSeq Version	NM_143568.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dj-1beta (dj-1beta), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143568.3

ORF Insert Sequence:

ATGTCGAAAA GCGCGCTGGT GATTTTGGCT CCCGGAGCGG AGGAGATGGA GTTCATCATA 
GCCGCCGACG TTCTGAGAAG GGCGGGCATC AAGGTCACCG TAGCCGGTTT GAATGGCGGG 
GAAGCGGTGA AGTGCTCGCG GGATGTGCAG ATCCTGCCGG ACACCTCGCT GGCCCAGGTT 
GCCTCGGATA AGTTCGATGT GGTGGTGCTG CCCGGCGGAC TGGGTGGCTC CAATGCCATG 
GGGGAGTCCT CGCTGGTCGG TGACTTGCTG CGCAGCCAGG AGTCTGGTGG CGGACTCATC 
GCCGCCATCT GTGCCGCGCC CACCGTTTTG GCCAAGCACG GCGTCGCCTC CGGGAAATCC 
CTCACCTCGT ATCCCTCAAT GAAGCCCCAG CTGGTGAATA ACTATAGCTA TGTGGACGAC 
AAGACGGTGG TCAAGGATGG CAATCTGATC ACCAGTCGAG GTCCTGGCAC CGCCTACGAG 
TTCGCCCTCA AAATCGCCGA GGAGCTGGCG GGCAAGGAGA AAGTCCAGGA GGTGGCCAAG 
GGTCTTCTTG TGGCCTACAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dj-1beta cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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