Axn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Axn
Entrez Gene ID	43565
Full Name	Axin
Synonyms	0442/30,APC,CG7926,D-Axin,DAxin,Daxin,Dmel\CG7926,GSK3beta,axin,axn,d-axin,dAXIN,dAxin,din,l(3)S044230
General protein information	Preferred Names Axin Names CG7926 gene product from transcript CG7926-RA Axn-PA Axn-PB Axn-PC Axn-PD Axn-PE Axn-PF Axn-PG CG7926-PA CG7926-PB CG7926-PC CG7926-PD CG7926-PE CG7926-PF CG7926-PG D-axin Dm axin axin axn dAxin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99D2-99D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_170459.3	NP_733338.1	axin, isoform C
NM_001144654.3	NP_001138126.1	axin, isoform D
NM_001276169.1	NP_001263098.1	axin, isoform G
NM_001170302.1	NP_001163773.1	axin, isoform E
NM_170457.3	NP_733336.1	axin, isoform A
NM_170458.2	NP_733337.1	axin, isoform B
NM_001276168.1	NP_001263097.1	axin, isoform F

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Axn	NP_733337.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002123	XP_320914.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that even in the unstimulated state, a pool of Axin forms membrane-proximal puncta that are dependent on Apc, and that membrane association regulates both Axin levels and Axin's role in the rapid activation of signaling that follows Wnt exposure.
Title: Dual Roles for Membrane Association of Drosophila Axin in Wnt Signaling.

The pool of ADP-ribosylated Axin, which is degraded under basal conditions, increases immediately following Wnt stimulation in both Drosophila and human cells.
Title: Wnt pathway activation by ADP-ribosylation.

Daxin physically interacts with DTNKS, and its protein levels are elevated in the absence of DTNKS in the eye discs.
Title: The Drosophila tankyrase regulates Wg signaling depending on the concentration of Daxin.

co-expression experiments reveal that APC displaces Dishevelled from Axin assemblies, promoting degradasome over signalosome formation in the absence of Wnts
Title: The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled.

Data show that Axin and Apc, but not GSK3, modulate the ligand-independent traffic of Notch.
Title: Modulation of the ligand-independent traffic of Notch by Axin and Apc contributes to the activation of Armadillo in Drosophila.

The following Axn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Axn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa08961	NM_001170302.1 Latest version!	Drosophila melanogaster axin, transcript variant E (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa06591	NM_170457.3 Latest version!	Drosophila melanogaster axin, transcript variant A (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06591	NM_170458.2 Latest version!	Drosophila melanogaster axin, transcript variant B (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06592	NM_001276169.1 Latest version!	Drosophila melanogaster axin, transcript variant G (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa06592	NM_170459.3 Latest version!	Drosophila melanogaster axin, transcript variant C (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08959	NM_001144654.3 Latest version!	Drosophila melanogaster axin, transcript variant D (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa08960	NM_001276168.1 Latest version!	Drosophila melanogaster axin, transcript variant F (Axn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Axn ( NM_001170302.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163773.1 Drosophila melanogaster axin, transcript variant E (Axn), mRNA.

Price & Availability↑

CloneID	OFa08961
Clone ID Related Accession (Same CDS sequence)	NM_001170302.1
Accession Version	NM_001170302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163773.1
CDS	404..736
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYR

Target ORF information:

RefSeq Version	NM_001170302.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant E (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170302.1

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axn ( NM_170457.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733336.1 Drosophila melanogaster axin, transcript variant A (Axn), mRNA.

Price & Availability↑

CloneID	OFa06591
Clone ID Related Accession (Same CDS sequence)	NM_170457.3 , NM_170458.2
Accession Version	NM_170457.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170457.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG TTCTCGATCT 
GGCTCTAGCA CGGCCTCGAA ACTGGAGGAG GCGAAACGAA GACTGGAAGA CGAGCCGCGG 
CGCTCTCGTC GATACGCCCA ACCGCCGATG CAGCACCTGT CGCAACAGCC ACTGGCCTCC 
TTCAGCAGCA GCAGCAGTGG CGGCAGCATC AGCCTGCCAC ACCAGCCACC GCCTCTGCCG 
GCCAAGCCGC CGGAGACGAT CGTGGTGTTC TCGTTCTGCG AGGAGCCAGT GCCGTACAGG 
ATAAAAATAC CCGGTACTCA GCCAACCCTG CGTCAGTTTA AGGACTATCT GCCCAGAAGA 
GGTCACTTTA GGTTCTTCTT CAAGACGCAC TGCGAGGACC CGGACAGTCC AGTGATTCAG 
GAAGAGATTG TTAACGACTC CGACATACTG CCGCTATTCG GAGACAAAGC GATGGGTCTT 
GTCAAGCCAT CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733336.1
CDS	1457..3694
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGAHGYVYNPSTTNTSYVPNSRVDSERASVSSGGRTDSDTMSISSCSMDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASSRSGSSTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD

Target ORF information:

RefSeq Version	NM_170457.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant A (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170457.3

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG TTCTCGATCT 
GGCTCTAGCA CGGCCTCGAA ACTGGAGGAG GCGAAACGAA GACTGGAAGA CGAGCCGCGG 
CGCTCTCGTC GATACGCCCA ACCGCCGATG CAGCACCTGT CGCAACAGCC ACTGGCCTCC 
TTCAGCAGCA GCAGCAGTGG CGGCAGCATC AGCCTGCCAC ACCAGCCACC GCCTCTGCCG 
GCCAAGCCGC CGGAGACGAT CGTGGTGTTC TCGTTCTGCG AGGAGCCAGT GCCGTACAGG 
ATAAAAATAC CCGGTACTCA GCCAACCCTG CGTCAGTTTA AGGACTATCT GCCCAGAAGA 
GGTCACTTTA GGTTCTTCTT CAAGACGCAC TGCGAGGACC CGGACAGTCC AGTGATTCAG 
GAAGAGATTG TTAACGACTC CGACATACTG CCGCTATTCG GAGACAAAGC GATGGGTCTT 
GTCAAGCCAT CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axn ( NM_170458.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733337.1 Drosophila melanogaster axin, transcript variant B (Axn), mRNA.

Price & Availability↑

CloneID	OFa06591
Clone ID Related Accession (Same CDS sequence)	NM_170457.3 , NM_170458.2
Accession Version	NM_170458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170458.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG TTCTCGATCT 
GGCTCTAGCA CGGCCTCGAA ACTGGAGGAG GCGAAACGAA GACTGGAAGA CGAGCCGCGG 
CGCTCTCGTC GATACGCCCA ACCGCCGATG CAGCACCTGT CGCAACAGCC ACTGGCCTCC 
TTCAGCAGCA GCAGCAGTGG CGGCAGCATC AGCCTGCCAC ACCAGCCACC GCCTCTGCCG 
GCCAAGCCGC CGGAGACGAT CGTGGTGTTC TCGTTCTGCG AGGAGCCAGT GCCGTACAGG 
ATAAAAATAC CCGGTACTCA GCCAACCCTG CGTCAGTTTA AGGACTATCT GCCCAGAAGA 
GGTCACTTTA GGTTCTTCTT CAAGACGCAC TGCGAGGACC CGGACAGTCC AGTGATTCAG 
GAAGAGATTG TTAACGACTC CGACATACTG CCGCTATTCG GAGACAAAGC GATGGGTCTT 
GTCAAGCCAT CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733337.1
CDS	404..2641
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGAHGYVYNPSTTNTSYVPNSRVDSERASVSSGGRTDSDTMSISSCSMDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASSRSGSSTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD

Target ORF information:

RefSeq Version	NM_170458.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant B (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170458.2

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG TTCTCGATCT 
GGCTCTAGCA CGGCCTCGAA ACTGGAGGAG GCGAAACGAA GACTGGAAGA CGAGCCGCGG 
CGCTCTCGTC GATACGCCCA ACCGCCGATG CAGCACCTGT CGCAACAGCC ACTGGCCTCC 
TTCAGCAGCA GCAGCAGTGG CGGCAGCATC AGCCTGCCAC ACCAGCCACC GCCTCTGCCG 
GCCAAGCCGC CGGAGACGAT CGTGGTGTTC TCGTTCTGCG AGGAGCCAGT GCCGTACAGG 
ATAAAAATAC CCGGTACTCA GCCAACCCTG CGTCAGTTTA AGGACTATCT GCCCAGAAGA 
GGTCACTTTA GGTTCTTCTT CAAGACGCAC TGCGAGGACC CGGACAGTCC AGTGATTCAG 
GAAGAGATTG TTAACGACTC CGACATACTG CCGCTATTCG GAGACAAAGC GATGGGTCTT 
GTCAAGCCAT CCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axn ( NM_170459.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733338.1 Drosophila melanogaster axin, transcript variant C (Axn), mRNA.

Price & Availability↑

CloneID	OFa06592
Clone ID Related Accession (Same CDS sequence)	NM_170459.3 , NM_001276169.1
Accession Version	NM_170459.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170459.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG CACGGCCTCG 
AAACTGGAGG AGGCGAAACG AAGACTGGAA GACGAGCCGC GGCGCTCTCG TCGATACGCC 
CAACCGCCGA TGCAGCACCT GTCGCAACAG CCACTGGCCT CCTTCAGCAG CAGCAGCAGT 
GGCGGCAGCA TCAGCCTGCC ACACCAGCCA CCGCCTCTGC CGGCCAAGCC GCCGGAGACG 
ATCGTGGTGT TCTCGTTCTG CGAGGAGCCA GTGCCGTACA GGATAAAAAT ACCCGGTACT 
CAGCCAACCC TGCGTCAGTT TAAGGACTAT CTGCCCAGAA GAGGTCACTT TAGGTTCTTC 
TTCAAGACGC ACTGCGAGGA CCCGGACAGT CCAGTGATTC AGGAAGAGAT TGTTAACGAC 
TCCGACATAC TGCCGCTATT CGGAGACAAA GCGATGGGTC TTGTCAAGCC ATCCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733338.1
CDS	1457..3676
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGAHGYVYNPSTTNTSYVPNSRVDSERASVSSGGRTDSDTMSISSCSMDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD

Target ORF information:

RefSeq Version	NM_170459.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant C (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170459.3

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG CACGGCCTCG 
AAACTGGAGG AGGCGAAACG AAGACTGGAA GACGAGCCGC GGCGCTCTCG TCGATACGCC 
CAACCGCCGA TGCAGCACCT GTCGCAACAG CCACTGGCCT CCTTCAGCAG CAGCAGCAGT 
GGCGGCAGCA TCAGCCTGCC ACACCAGCCA CCGCCTCTGC CGGCCAAGCC GCCGGAGACG 
ATCGTGGTGT TCTCGTTCTG CGAGGAGCCA GTGCCGTACA GGATAAAAAT ACCCGGTACT 
CAGCCAACCC TGCGTCAGTT TAAGGACTAT CTGCCCAGAA GAGGTCACTT TAGGTTCTTC 
TTCAAGACGC ACTGCGAGGA CCCGGACAGT CCAGTGATTC AGGAAGAGAT TGTTAACGAC 
TCCGACATAC TGCCGCTATT CGGAGACAAA GCGATGGGTC TTGTCAAGCC ATCCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axn ( NM_001276169.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263098.1 Drosophila melanogaster axin, transcript variant G (Axn), mRNA.

Price & Availability↑

CloneID	OFa06592
Clone ID Related Accession (Same CDS sequence)	NM_170459.3 , NM_001276169.1
Accession Version	NM_001276169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG CACGGCCTCG 
AAACTGGAGG AGGCGAAACG AAGACTGGAA GACGAGCCGC GGCGCTCTCG TCGATACGCC 
CAACCGCCGA TGCAGCACCT GTCGCAACAG CCACTGGCCT CCTTCAGCAG CAGCAGCAGT 
GGCGGCAGCA TCAGCCTGCC ACACCAGCCA CCGCCTCTGC CGGCCAAGCC GCCGGAGACG 
ATCGTGGTGT TCTCGTTCTG CGAGGAGCCA GTGCCGTACA GGATAAAAAT ACCCGGTACT 
CAGCCAACCC TGCGTCAGTT TAAGGACTAT CTGCCCAGAA GAGGTCACTT TAGGTTCTTC 
TTCAAGACGC ACTGCGAGGA CCCGGACAGT CCAGTGATTC AGGAAGAGAT TGTTAACGAC 
TCCGACATAC TGCCGCTATT CGGAGACAAA GCGATGGGTC TTGTCAAGCC ATCCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263098.1
CDS	404..2623
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGAHGYVYNPSTTNTSYVPNSRVDSERASVSSGGRTDSDTMSISSCSMDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD

Target ORF information:

RefSeq Version	NM_001276169.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant G (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276169.1

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGT 
GCCCACGGTT ACGTGTACAA TCCAAGCACT ACCAACACCT CCTATGTGCC GAATTCGCGG 
GTTGATTCGG AGAGGGCCAG CGTTAGCTCC GGCGGGCGCA CCGACTCCGA CACCATGTCG 
ATCTCCAGCT GTTCCATGGA CGGTCGCCCG TACATCCAAC GCAGACACAG CTCCACGGAG 
AGCAAGGCGA TCCGCCAGAG CGCGATGGCA AACAAGGAGA CCAACACCTT TCAGGTGATA 
CCTCGCACAC AACGACTACA TTCGAACGAG CACAGACCAC TGAAGGAAGA GGAGCTGGTG 
TCCTTGCTGA TACCCAAGTT GGAGGAAGTG AAGCGAAAGC GAGACCTGGA GGAAAGAGCT 
CGCGAGAGAA ATCCCGGTGC TGCTCTGCTT ACCAACGAAA GATCCAGTGC CAGCGATCGA 
GCCTTCGCCG AGGCGATACG AGAGAAGTTT GCACTGGACG AAGACAACGA CCAAGACATT 
CTGGACCAGC ATGTGTCGCG GGTGTGGAAG GACCAAACGC CGCACCGCTC GCCGGGAACG 
ATGTCGCCCT GCCCACCCAT ACCGTCGCGC CGACGTACAG CCACCCACGA TTCCGGAATG 
GTCAGCGATG GCGCCATGAG TCTAAGCGGA CACTCAATGA AGCACTCAAA GTCCATGCCG 
GATCACAGTT CCTGCTCGAG GAAGTTGACA AACAAATGGC CTTCCATGAA CACAGACAGT 
GGCATTAGCA TGTTCTCGGC CGACACTGTT ACCAAGTACA AAGATGCGAG CACGGCCTCG 
AAACTGGAGG AGGCGAAACG AAGACTGGAA GACGAGCCGC GGCGCTCTCG TCGATACGCC 
CAACCGCCGA TGCAGCACCT GTCGCAACAG CCACTGGCCT CCTTCAGCAG CAGCAGCAGT 
GGCGGCAGCA TCAGCCTGCC ACACCAGCCA CCGCCTCTGC CGGCCAAGCC GCCGGAGACG 
ATCGTGGTGT TCTCGTTCTG CGAGGAGCCA GTGCCGTACA GGATAAAAAT ACCCGGTACT 
CAGCCAACCC TGCGTCAGTT TAAGGACTAT CTGCCCAGAA GAGGTCACTT TAGGTTCTTC 
TTCAAGACGC ACTGCGAGGA CCCGGACAGT CCAGTGATTC AGGAAGAGAT TGTTAACGAC 
TCCGACATAC TGCCGCTATT CGGAGACAAA GCGATGGGTC TTGTCAAGCC ATCCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axn ( NM_001144654.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138126.1 Drosophila melanogaster axin, transcript variant D (Axn), mRNA.

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CloneID	OFa08959
Clone ID Related Accession (Same CDS sequence)	NM_001144654.3
Accession Version	NM_001144654.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144654.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGG 
GACGGTCGCC CGTACATCCA ACGCAGACAC AGCTCCACGG AGAGCAAGGC GATCCGCCAG 
AGCGCGATGG CAAACAAGGA GACCAACACC TTTCAGGTGA TACCTCGCAC ACAACGACTA 
CATTCGAACG AGCACAGACC ACTGAAGGAA GAGGAGCTGG TGTCCTTGCT GATACCCAAG 
TTGGAGGAAG TGAAGCGAAA GCGAGACCTG GAGGAAAGAG CTCGCGAGAG AAATCCCGGT 
GCTGCTCTGC TTACCAACGA AAGATCCAGT GCCAGCGATC GAGCCTTCGC CGAGGCGATA 
CGAGAGAAGT TTGCACTGGA CGAAGACAAC GACCAAGACA TTCTGGACCA GCATGTGTCG 
CGGGTGTGGA AGGACCAAAC GCCGCACCGC TCGCCGGGAA CGATGTCGCC CTGCCCACCC 
ATACCGTCGC GCCGACGTAC AGCCACCCAC GATTCCGGAA TGGTCAGCGA TGGCGCCATG 
AGTCTAAGCG GACACTCAAT GAAGCACTCA AAGTCCATGC CGGATCACAG TTCCTGCTCG 
AGGAAGTTGA CAAACAAATG GCCTTCCATG AACACAGACA GTGGCATTAG CATGTTCTCG 
GCCGACACTG TTACCAAGTA CAAAGATGCG AGTTCTCGAT CTGGCTCTAG CACGGCCTCG 
AAACTGGAGG AGGCGAAACG AAGACTGGAA GACGAGCCGC GGCGCTCTCG TCGATACGCC 
CAACCGCCGA TGCAGCACCT GTCGCAACAG CCACTGGCCT CCTTCAGCAG CAGCAGCAGT 
GGCGGCAGCA TCAGCCTGCC ACACCAGCCA CCGCCTCTGC CGGCCAAGCC GCCGGAGACG 
ATCGTGGTGT TCTCGTTCTG CGAGGAGCCA GTGCCGTACA GGATAAAAAT ACCCGGTACT 
CAGCCAACCC TGCGTCAGTT TAAGGACTAT CTGCCCAGAA GAGGTCACTT TAGGTTCTTC 
TTCAAGACGC ACTGCGAGGA CCCGGACAGT CCAGTGATTC AGGAAGAGAT TGTTAACGAC 
TCCGACATAC TGCCGCTATT CGGAGACAAA GCGATGGGTC TTGTCAAGCC ATCCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138126.1
CDS	1457..3556
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASSRSGSSTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD

Target ORF information:

RefSeq Version	NM_001144654.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant D (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144654.3

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGG 
GACGGTCGCC CGTACATCCA ACGCAGACAC AGCTCCACGG AGAGCAAGGC GATCCGCCAG 
AGCGCGATGG CAAACAAGGA GACCAACACC TTTCAGGTGA TACCTCGCAC ACAACGACTA 
CATTCGAACG AGCACAGACC ACTGAAGGAA GAGGAGCTGG TGTCCTTGCT GATACCCAAG 
TTGGAGGAAG TGAAGCGAAA GCGAGACCTG GAGGAAAGAG CTCGCGAGAG AAATCCCGGT 
GCTGCTCTGC TTACCAACGA AAGATCCAGT GCCAGCGATC GAGCCTTCGC CGAGGCGATA 
CGAGAGAAGT TTGCACTGGA CGAAGACAAC GACCAAGACA TTCTGGACCA GCATGTGTCG 
CGGGTGTGGA AGGACCAAAC GCCGCACCGC TCGCCGGGAA CGATGTCGCC CTGCCCACCC 
ATACCGTCGC GCCGACGTAC AGCCACCCAC GATTCCGGAA TGGTCAGCGA TGGCGCCATG 
AGTCTAAGCG GACACTCAAT GAAGCACTCA AAGTCCATGC CGGATCACAG TTCCTGCTCG 
AGGAAGTTGA CAAACAAATG GCCTTCCATG AACACAGACA GTGGCATTAG CATGTTCTCG 
GCCGACACTG TTACCAAGTA CAAAGATGCG AGTTCTCGAT CTGGCTCTAG CACGGCCTCG 
AAACTGGAGG AGGCGAAACG AAGACTGGAA GACGAGCCGC GGCGCTCTCG TCGATACGCC 
CAACCGCCGA TGCAGCACCT GTCGCAACAG CCACTGGCCT CCTTCAGCAG CAGCAGCAGT 
GGCGGCAGCA TCAGCCTGCC ACACCAGCCA CCGCCTCTGC CGGCCAAGCC GCCGGAGACG 
ATCGTGGTGT TCTCGTTCTG CGAGGAGCCA GTGCCGTACA GGATAAAAAT ACCCGGTACT 
CAGCCAACCC TGCGTCAGTT TAAGGACTAT CTGCCCAGAA GAGGTCACTT TAGGTTCTTC 
TTCAAGACGC ACTGCGAGGA CCCGGACAGT CCAGTGATTC AGGAAGAGAT TGTTAACGAC 
TCCGACATAC TGCCGCTATT CGGAGACAAA GCGATGGGTC TTGTCAAGCC ATCCGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Axn ( NM_001276168.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263097.1 Drosophila melanogaster axin, transcript variant F (Axn), mRNA.

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CloneID	OFa08960
Clone ID Related Accession (Same CDS sequence)	NM_001276168.1
Accession Version	NM_001276168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	axin, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGG 
GACGGTCGCC CGTACATCCA ACGCAGACAC AGCTCCACGG AGAGCAAGGC GATCCGCCAG 
AGCGCGATGG CAAACAAGGA GACCAACACC TTTCAGGTGA TACCTCGCAC ACAACGACTA 
CATTCGAACG AGCACAGACC ACTGAAGGAA GAGGAGCTGG TGTCCTTGCT GATACCCAAG 
TTGGAGGAAG TGAAGCGAAA GCGAGACCTG GAGGAAAGAG CTCGCGAGAG AAATCCCGGT 
GCTGCTCTGC TTACCAACGA AAGATCCAGT GCCAGCGATC GAGCCTTCGC CGAGGCGATA 
CGAGAGAAGT TTGCACTGGA CGAAGACAAC GACCAAGACA TTCTGGACCA GCATGTGTCG 
CGGGTGTGGA AGGACCAAAC GCCGCACCGC TCGCCGGGAA CGATGTCGCC CTGCCCACCC 
ATACCGTCGC GCCGACGTAC AGCCACCCAC GATTCCGGAA TGGTCAGCGA TGGCGCCATG 
AGTCTAAGCG GACACTCAAT GAAGCACTCA AAGTCCATGC CGGATCACAG TTCCTGCTCG 
AGGAAGTTGA CAAACAAATG GCCTTCCATG AACACAGACA GTGGCATTAG CATGTTCTCG 
GCCGACACTG TTACCAAGTA CAAAGATGCG AGCACGGCCT CGAAACTGGA GGAGGCGAAA 
CGAAGACTGG AAGACGAGCC GCGGCGCTCT CGTCGATACG CCCAACCGCC GATGCAGCAC 
CTGTCGCAAC AGCCACTGGC CTCCTTCAGC AGCAGCAGCA GTGGCGGCAG CATCAGCCTG 
CCACACCAGC CACCGCCTCT GCCGGCCAAG CCGCCGGAGA CGATCGTGGT GTTCTCGTTC 
TGCGAGGAGC CAGTGCCGTA CAGGATAAAA ATACCCGGTA CTCAGCCAAC CCTGCGTCAG 
TTTAAGGACT ATCTGCCCAG AAGAGGTCAC TTTAGGTTCT TCTTCAAGAC GCACTGCGAG 
GACCCGGACA GTCCAGTGAT TCAGGAAGAG ATTGTTAACG ACTCCGACAT ACTGCCGCTA 
TTCGGAGACA AAGCGATGGG TCTTGTCAAG CCATCCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263097.1
CDS	1457..3538
Translation	MSGHPSGIRKHDDNECSGPRPPVPGEESRVKKMTEGVADTSKNSSPSYLNWARTLNHLLEDRDGVELFKKYVEEEAPAYNDHLNFYFACEGLKQQTDPEKIKQIIGAIYRFLRKSQLSISDDLRAQIKAIKTNPEIPLSPHIFDPMQRHVEVTIRDNIYPTFLCSEMYILYIQQMSAQQERCTSSGATGSGSAGSSGSGGSSLAGACALPPTTASGKQQLPQLVPPGAFINLPVSSVSGPPAGTCSASGSVYGPSTSASSSGSISATDTLPRSSTLPTLHEDSVLSLCDDFEKVQMQEGGGSLGSGSVGAGARAPDYPIRLTRDLLIATQKRRLEIRPPGDGRPYIQRRHSSTESKAIRQSAMANKETNTFQVIPRTQRLHSNEHRPLKEEELVSLLIPKLEEVKRKRDLEERARERNPGAALLTNERSSASDRAFAEAIREKFALDEDNDQDILDQHVSRVWKDQTPHRSPGTMSPCPPIPSRRRTATHDSGMVSDGAMSLSGHSMKHSKSMPDHSSCSRKLTNKWPSMNTDSGISMFSADTVTKYKDASTASKLEEAKRRLEDEPRRSRRYAQPPMQHLSQQPLASFSSSSSGGSISLPHQPPPLPAKPPETIVVFSFCEEPVPYRIKIPGTQPTLRQFKDYLPRRGHFRFFFKTHCEDPDSPVIQEEIVNDSDILPLFGDKAMGLVKPSD

Target ORF information:

RefSeq Version	NM_001276168.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster axin, transcript variant F (Axn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276168.1

ORF Insert Sequence:

ATGAGTGGCC ATCCATCGGG AATCCGGAAA CATGATGATA ATGAGTGTAG TGGCCCACGA 
CCACCAGTAC CCGGAGAAGA GAGCCGTGTT AAAAAGATGA CCGAAGGGGT AGCAGATACC 
TCGAAGAATT CGTCGCCATC CTACCTGAAC TGGGCCCGGA CGCTGAACCA CCTGCTGGAG 
GACCGCGATG GGGTCGAGCT CTTTAAGAAG TACGTGGAGG AGGAGGCGCC CGCCTACAAC 
GACCACCTCA ACTTCTACTT CGCCTGCGAG GGCCTCAAGC AGCAGACGGA TCCGGAGAAA 
ATCAAGCAGA TAATCGGAGC CATCTACAGA TTCTTGCGCA AGAGTCAGCT GTCCATCTCC 
GATGACCTGC GCGCCCAGAT CAAGGCCATC AAGACGAATC CCGAGATCCC GCTCAGCCCA 
CACATATTCG ATCCCATGCA GCGCCATGTG GAGGTCACCA TCCGCGACAA CATCTACCCC 
ACGTTTCTCT GCTCCGAGAT GTACATCCTC TACATCCAGC AGATGTCCGC CCAGCAGGAG 
CGCTGCACCA GCAGCGGCGC CACGGGATCG GGAAGTGCCG GGAGTAGTGG CAGCGGCGGC 
AGCAGCCTAG CGGGTGCCTG TGCCCTGCCA CCGACCACCG CGTCCGGCAA GCAGCAGCTC 
CCGCAGCTGG TGCCGCCCGG TGCCTTCATC AACCTGCCGG TGAGCAGCGT GAGCGGGCCG 
CCAGCTGGCA CGTGCAGTGC CAGCGGCAGT GTGTACGGTC CCTCGACTTC GGCCAGCTCC 
AGCGGCTCAA TAAGCGCCAC CGACACACTG CCCCGCAGCT CCACGCTGCC CACGCTGCAC 
GAGGACTCGG TGCTGTCGCT CTGCGACGAC TTCGAGAAGG TGCAGATGCA GGAGGGCGGC 
GGATCCCTCG GCTCTGGATC TGTGGGCGCG GGTGCGCGAG CGCCCGACTA CCCGATTCGC 
CTCACGCGGG ACCTACTAAT AGCAACTCAG AAAAGAAGAC TGGAAATCCG ACCTCCTGGG 
GACGGTCGCC CGTACATCCA ACGCAGACAC AGCTCCACGG AGAGCAAGGC GATCCGCCAG 
AGCGCGATGG CAAACAAGGA GACCAACACC TTTCAGGTGA TACCTCGCAC ACAACGACTA 
CATTCGAACG AGCACAGACC ACTGAAGGAA GAGGAGCTGG TGTCCTTGCT GATACCCAAG 
TTGGAGGAAG TGAAGCGAAA GCGAGACCTG GAGGAAAGAG CTCGCGAGAG AAATCCCGGT 
GCTGCTCTGC TTACCAACGA AAGATCCAGT GCCAGCGATC GAGCCTTCGC CGAGGCGATA 
CGAGAGAAGT TTGCACTGGA CGAAGACAAC GACCAAGACA TTCTGGACCA GCATGTGTCG 
CGGGTGTGGA AGGACCAAAC GCCGCACCGC TCGCCGGGAA CGATGTCGCC CTGCCCACCC 
ATACCGTCGC GCCGACGTAC AGCCACCCAC GATTCCGGAA TGGTCAGCGA TGGCGCCATG 
AGTCTAAGCG GACACTCAAT GAAGCACTCA AAGTCCATGC CGGATCACAG TTCCTGCTCG 
AGGAAGTTGA CAAACAAATG GCCTTCCATG AACACAGACA GTGGCATTAG CATGTTCTCG 
GCCGACACTG TTACCAAGTA CAAAGATGCG AGCACGGCCT CGAAACTGGA GGAGGCGAAA 
CGAAGACTGG AAGACGAGCC GCGGCGCTCT CGTCGATACG CCCAACCGCC GATGCAGCAC 
CTGTCGCAAC AGCCACTGGC CTCCTTCAGC AGCAGCAGCA GTGGCGGCAG CATCAGCCTG 
CCACACCAGC CACCGCCTCT GCCGGCCAAG CCGCCGGAGA CGATCGTGGT GTTCTCGTTC 
TGCGAGGAGC CAGTGCCGTA CAGGATAAAA ATACCCGGTA CTCAGCCAAC CCTGCGTCAG 
TTTAAGGACT ATCTGCCCAG AAGAGGTCAC TTTAGGTTCT TCTTCAAGAC GCACTGCGAG 
GACCCGGACA GTCCAGTGAT TCAGGAAGAG ATTGTTAACG ACTCCGACAT ACTGCCGCTA 
TTCGGAGACA AAGCGATGGG TCTTGTCAAG CCATCCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Axn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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