trp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	trp
Entrez Gene ID	43542
Full Name	transient receptor potential
Synonyms	559,CG7875,DmTRP,Dmel\CG7875,TRP,Trp,dTRP,dTrp,gene B,idl-B
General protein information	Preferred Names transient receptor potential Names CG7875 gene product from transcript CG7875-RA CG7875-PA CG7875-PB Cosens-Manning mutant INAD localization-B transient photreceptor potential transient receptor potential protein trp-PA trp-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99C6-99C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_057420.4	NP_476768.1	transient receptor potential, isoform A
NM_001276161.1	NP_001263090.1	transient receptor potential, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	trp	NP_476768.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000348	XP_310766.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TRP de-phosphorylation affects the detection limit of oscillating light in photoreceptor cells.
Title: The Phosphorylation State of the

TRP and TRPL assemble exclusively as homomultimeric channels in their native environment.
Title: Drosophila TRP and TRPL are assembled as homomultimeric channels in vivo.

Data show that phosphorylation of most ransient receptor potential (TRP) ion channel sites depends on the phototransduction cascade and the activity of the TRP.
Title: Phosphorylation of the Drosophila transient receptor potential ion channel is regulated by the phototransduction cascade and involves several protein kinases and phosphatases.

These results indicate that the Pain TRP channels in the MBs and IPCs control neuronal plasticity that is required for the formation of a certain type of long-lasting associative memory in Drosophila.
Title: Significance of the centrally expressed TRP channel painless in Drosophila courtship memory.

Vesicle exocytosis depends on cacophony, TRP and TRPL.
Title: TRP, TRPL and cacophony channels mediate Ca2+ influx and exocytosis in photoreceptors axons in Drosophila.

The following trp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08021	NM_001276161.1 Latest version!	Drosophila melanogaster transient receptor potential (trp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa06566	NM_057420.4 Latest version!	Drosophila melanogaster transient receptor potential (trp), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trp ( NM_001276161.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263090.1 Drosophila melanogaster transient receptor potential (trp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa08021
Clone ID Related Accession (Same CDS sequence)	NM_001276161.1
Accession Version	NM_001276161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAGCA ATACGGAATC CGATGCCGAG AAGGCGTTGG GGTCTCGCCT GGATTACGAC 
CTGATGATGG CCGAGGAGTA CATCCTCAGC GATGTGGAGA AGAATTTCAT ATTGTCCTGC 
GAGCGGGGTG ACTTGCCAGG TGTCAAGAAG ATCCTCGAGG AGTACCAGGG CACGGACAAG 
TTCAACATTA ACTGCACGGA TCCCATGAAC CGCTCCGCCC TCATTTCGGC CATCGAGAAC 
GAGAACTTCG ACCTGATGGT GATCCTGCTG GAGCATAACA TCGAGGTGGG CGACGCCCTG 
TTGCACGCCA TCTCGGAGGA GTATGTGGAG GCGGTGGAGG AGCTGCTGCA GTGGGAGGAG 
ACCAACCACA AGGAGGGCCA GCCATACAGC TGGGAGGCGG TGGACCGCTC CAAGTCCACC 
TTCACCGTGG ACATCACGCC CCTTATCCTG GCCGCCCACC GAAATAACTA CGAGATACTC 
AAAATCCTCC TGGATCGCGG GGCCACGCTG CCCATGCCGC ACGACGTCAA GTGCGGCTGC 
GATGAGTGTG TGACCTCCCA GATGACGGAC TCCCTGCGCC ACTCGCAGTC GAGGATCAAC 
GCATACCGCG CCCTGTCCGC CAGCTCGCTG ATAGCGCTCA GCTCCCGGGA CCCTGTACTG 
ACCGCCTTCC AATTGTCCTG GGAACTCAAG CGCCTGCAGG CGATGGAATC GGAGTTTCGT 
GCCGAATACA CGGTACGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263090.1
CDS	227..967
Translation	MGSNTESDAEKALGSRLDYDLMMAEEYILSDVEKNFILSCERGDLPGVKKILEEYQGTDKFNINCTDPMNRSALISAIENENFDLMVILLEHNIEVGDALLHAISEEYVEAVEELLQWEETNHKEGQPYSWEAVDRSKSTFTVDITPLILAAHRNNYEILKILLDRGATLPMPHDVKCGCDECVTSQMTDSLRHSQSRINAYRALSASSLIALSSRDPVLTAFQLSWELKRLQAMESEFRAEYTVR

Target ORF information:

RefSeq Version	NM_001276161.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential (trp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276161.1

ORF Insert Sequence:

ATGGGCAGCA ATACGGAATC CGATGCCGAG AAGGCGTTGG GGTCTCGCCT GGATTACGAC 
CTGATGATGG CCGAGGAGTA CATCCTCAGC GATGTGGAGA AGAATTTCAT ATTGTCCTGC 
GAGCGGGGTG ACTTGCCAGG TGTCAAGAAG ATCCTCGAGG AGTACCAGGG CACGGACAAG 
TTCAACATTA ACTGCACGGA TCCCATGAAC CGCTCCGCCC TCATTTCGGC CATCGAGAAC 
GAGAACTTCG ACCTGATGGT GATCCTGCTG GAGCATAACA TCGAGGTGGG CGACGCCCTG 
TTGCACGCCA TCTCGGAGGA GTATGTGGAG GCGGTGGAGG AGCTGCTGCA GTGGGAGGAG 
ACCAACCACA AGGAGGGCCA GCCATACAGC TGGGAGGCGG TGGACCGCTC CAAGTCCACC 
TTCACCGTGG ACATCACGCC CCTTATCCTG GCCGCCCACC GAAATAACTA CGAGATACTC 
AAAATCCTCC TGGATCGCGG GGCCACGCTG CCCATGCCGC ACGACGTCAA GTGCGGCTGC 
GATGAGTGTG TGACCTCCCA GATGACGGAC TCCCTGCGCC ACTCGCAGTC GAGGATCAAC 
GCATACCGCG CCCTGTCCGC CAGCTCGCTG ATAGCGCTCA GCTCCCGGGA CCCTGTACTG 
ACCGCCTTCC AATTGTCCTG GGAACTCAAG CGCCTGCAGG CGATGGAATC GGAGTTTCGT 
GCCGAATACA CGGTACGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trp ( NM_057420.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476768.1 Drosophila melanogaster transient receptor potential (trp), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa06566
Clone ID Related Accession (Same CDS sequence)	NM_057420.4
Accession Version	NM_057420.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	transient receptor potential, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057420.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAGCA ATACGGAATC CGATGCCGAG AAGGCGTTGG GGTCTCGCCT GGATTACGAC 
CTGATGATGG CCGAGGAGTA CATCCTCAGC GATGTGGAGA AGAATTTCAT ATTGTCCTGC 
GAGCGGGGTG ACTTGCCAGG TGTCAAGAAG ATCCTCGAGG AGTACCAGGG CACGGACAAG 
TTCAACATTA ACTGCACGGA TCCCATGAAC CGCTCCGCCC TCATTTCGGC CATCGAGAAC 
GAGAACTTCG ACCTGATGGT GATCCTGCTG GAGCATAACA TCGAGGTGGG CGACGCCCTG 
TTGCACGCCA TCTCGGAGGA GTATGTGGAG GCGGTGGAGG AGCTGCTGCA GTGGGAGGAG 
ACCAACCACA AGGAGGGCCA GCCATACAGC TGGGAGGCGG TGGACCGCTC CAAGTCCACC 
TTCACCGTGG ACATCACGCC CCTTATCCTG GCCGCCCACC GAAATAACTA CGAGATACTC 
AAAATCCTCC TGGATCGCGG GGCCACGCTG CCCATGCCGC ACGACGTCAA GTGCGGCTGC 
GATGAGTGTG TGACCTCCCA GATGACGGAC TCCCTGCGCC ACTCGCAGTC GAGGATCAAC 
GCATACCGCG CCCTGTCCGC CAGCTCGCTG ATAGCGCTCA GCTCCCGGGA CCCTGTACTG 
ACCGCCTTCC AATTGTCCTG GGAACTCAAG CGCCTGCAGG CGATGGAATC GGAGTTTCGT 
GCCGAATACA CGGAGATGCG TCAGATGGTG CAGGACTTCG GGACCTCGCT CCTGGATCAC 
GCACGCACAT CCATGGAACT CGAGGTGATG CTCAACTTCA ACCACGAGCC GTCCCACGAC 
ATCTGGTGCC TTGGCCAGCG GCAAACCCTG GAACGACTGA AGCTGGCCAT TCGCTATAAG 
CAAAAAACGT TTGTGGCACA TCCAAATGTC CAACAATTGT TGGCCGCCAT TTGGTACGAC 
GGACTGCCGG GCTTCCGGCG CAAGCAGGCC TCCCAGCAGC TGATGGATGT CGTGAAGCTG 
GGATGCAGCT TCCCCATCTA CAGCTTGAAG TACATCCTGG CCCCGGATTC CGAGGGTGCC 
AAGTTCATGC GCAAGCCCTT TGTCAAGTTC ATCACGCACT CCTGCTCCTA CATGTTCTTC 
CTGATGCTCC TGGGTGCTGC CTCCCTGAGG GTGGTGCAAA TCACCTTTGA ACTCCTCGCA 
TTTCCCTGGA TGCTGACCAT GCTGGAGGAT TGGCGCAAAC ACGAGAGAGG TTCACTACCG 
GGTCCCATTG AACTGGCAAT CATTACCTAC ATAATGGCTC TAATATTTGA GGAACTGAAA 
TCTTTATATT CGGACGGCTT GTTTGAGTAC ATCATGGATC TTTGGAACAT AGTGGACTAC 
ATATCGAACA TGTTCTATGT GACGTGGATT CTTTGTAGGG CCACCGCTTG GGTAATCGTC 
CATCGTGATC TCTGGTTCCG GGGCATAGAT CCTTACTTCC CAAGGGAACA CTGGCATCCG 
TTTGATCCAA TGCTTCTATC AGAGGGCGCC TTTGCTGCCG GAATGGTCTT CTCCTATCTA 
AAGCTCGTCC ACATCTTCTC CATTAATCCC CACCTGGGAC CCTTGCAAGT TTCACTGGGT 
CGCATGATAA TCGACATCAT CAAGTTCTTC TTCATCTACA CACTGGTGTT GTTTGCCTTC 
GGATGTGGTC TCAACCAGTT GCTATGGTAC TACGCTGAGC TGGAGAAGAA CAAGTGCTAT 
CACCTGCATC CCGATGTGGC TGACTTTGAT GACCAGGAGA AGGCTTGTAC CATCTGGCGA 
AGATTTTCCA ACTTATTCGA AACATCACAA TCGCTCTTTT GGGCCTCTTT TGGCCTGGTG 
GACCTGGTCT CCTTCGATCT GGCGGGAATC AAGAGCTTCA CCCGCTTCTG GGCACTGCTA 
ATGTTCGGCT CCTATTCGGT TATCAACATC ATTGTGCTTC TCAACATGCT GATTGCCATG 
ATGTCCAACT CCTACCAAAT CATCTCGGAG CGAGCCGACA CTGAGTGGAA GTTCGCCCGA 
TCCCAGCTGT GGATGAGCTA CTTCGAGGAT GGCGGCACCA TTCCACCGCC CTTCAACCTC 
TGTCCCAACA TGAAGATGTT GAGGAAGACC CTGGGCCGAA AGCGACCGTC ACGAACCAAG 
AGCTTCATGC GAAAGTCCAT GGAACGGGCA CAGACGCTGC ATGACAAAGT GATGAAGCTG 
CTGGTCAGGA GGTACATTAC GGCGGAGCAG AGGCGGCGGG ACGATTACGG CATTACCGAG 
GATGATATCA TTGAGGTGCG CCAGGACATC AGCTCCTTGC GGTTCGAGTT GCTGGAGATT 
TTCACCAACA ATAACTGGGA TGTACCCGAC ATTGAGAAGA AGTCGCAGGG AGTTGCTCGA 
ACCACCAAGG GCAAGGTGAT GGAACGTCGC ATCCTTAAGG ACTTCCAAAT CGGCTTCGTC 
GAGAATCTGA AGCAGGAAAT GAGCGAATCT GAGAGCGGAC GAGATATATT CTCATCGCTG 
GCCAAGGTCA TCGGCAGAAA GAAGACCCAG AAGGGAGACA AGGATTGGAA CGCCATTGCG 
AGGAAGAATA CTTTCGCCTC CGATCCCATT GGCTCCAAGC GCTCCTCCAT GCAACGTCAT 
AGCCAGCGAA GCTTGAGGAG GAAGATCATC GAGCAGGCGA ATGAGGGTCT TCAGATGAAC 
CAGACCCAGT TGATTGAATT CAATCCCAAC TTGGGTGATG TGACGCGTGC CACAAGAGTG 
GCTTATGTCA AGTTCATGCG GAAGAAGATG GCTGCCGACG AGGTTTCCTT GGCCGATGAC 
GAGGGTGCTC CAAATGGCGA AGGCGAAAAG AAGCCACTGG ATGCCTCTGG GTCTAAAAAG 
TCCATAACTA GTGGTGGAAC TGGAGGAGGA GCTTCTATGT TGGCTGCAGC TGCTCTAAGA 
GCATCGGTTA AGAATGTGGA TGAAAAATCC GGAGCCGATG GCAAGCCCGG CACGATGGGC 
AAGCCAACGG ATGACAAGAA AGCAGGTGAT GATAAGGATA AGCAGCAGCC TCCCAAGGAC 
TCCAAGCCGT CAGCAGGTGG TCCCAAGCCC GGGGATCAGA AGCCAACTCC GGGTGCGGGA 
GCTCCAAAGC CCCAAGCAGC TGGCACTATC AGCAAGCCCG GTGAGTCACA AAAGAAGGAC 
GCTCCGGCAC CACCAACCAA ACCTGGAGAC ACCAAGCCTG CTGCGCCGAA GCCTGGAGAA 
TCCGCCAAGC CCGAGGCCGC TGCCAAAAAG GAGGAGTCTT CCAAGACCGA AGCTAGCAAG 
CCGGCAGCCA CAAATGGAGC AGCCAAGAGC GCAGCTCCCT CCGCTCCTTC GGATGCCAAG 
CCGGATTCCA AACTGAAACC TGGAGCAGCT GGAGCACCAG AAGCAACTAA GGCAACCAAT 
GGGGCCTCCA AGCCGGACGA AAAGAAGAGC GGTCCGGAGG AGCCAAAAAA GGCTGCAGGA 
GACTCCAAGC CAGGAGACGA TGCCAAGGAC AAGGATAAGA AACCCGGCGA CGATAAGGAC 
AAGAAACCTG GCGACGACAA AGACAAGAAA CCTGCTGACA ATAATGATAA GAAGCCAGCC 
GATGACAAGG ACAAGAAGCC GGGAGACGAT AAGGACAAGA AGCCGGGTGA CGACAAGGAC 
AAGAAGCCGA GCGATGATAA GGACAAGAAG CCTGCCGATG ACAAGGACAA GAAGCCAGCA 
GCAGCTCCTC TGAAGCCGGC GATCAAGGTG GGTCAGAGCA GTGCCGCAGC TGGCGGAGAA 
CGAGGCAAAT CCACGGTCAC AGGACGCATG ATCTCCGGCT GGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476768.1
CDS	227..4054
Translation	MGSNTESDAEKALGSRLDYDLMMAEEYILSDVEKNFILSCERGDLPGVKKILEEYQGTDKFNINCTDPMNRSALISAIENENFDLMVILLEHNIEVGDALLHAISEEYVEAVEELLQWEETNHKEGQPYSWEAVDRSKSTFTVDITPLILAAHRNNYEILKILLDRGATLPMPHDVKCGCDECVTSQMTDSLRHSQSRINAYRALSASSLIALSSRDPVLTAFQLSWELKRLQAMESEFRAEYTEMRQMVQDFGTSLLDHARTSMELEVMLNFNHEPSHDIWCLGQRQTLERLKLAIRYKQKTFVAHPNVQQLLAAIWYDGLPGFRRKQASQQLMDVVKLGCSFPIYSLKYILAPDSEGAKFMRKPFVKFITHSCSYMFFLMLLGAASLRVVQITFELLAFPWMLTMLEDWRKHERGSLPGPIELAIITYIMALIFEELKSLYSDGLFEYIMDLWNIVDYISNMFYVTWILCRATAWVIVHRDLWFRGIDPYFPREHWHPFDPMLLSEGAFAAGMVFSYLKLVHIFSINPHLGPLQVSLGRMIIDIIKFFFIYTLVLFAFGCGLNQLLWYYAELEKNKCYHLHPDVADFDDQEKACTIWRRFSNLFETSQSLFWASFGLVDLVSFDLAGIKSFTRFWALLMFGSYSVINIIVLLNMLIAMMSNSYQIISERADTEWKFARSQLWMSYFEDGGTIPPPFNLCPNMKMLRKTLGRKRPSRTKSFMRKSMERAQTLHDKVMKLLVRRYITAEQRRRDDYGITEDDIIEVRQDISSLRFELLEIFTNNNWDVPDIEKKSQGVARTTKGKVMERRILKDFQIGFVENLKQEMSESESGRDIFSSLAKVIGRKKTQKGDKDWNAIARKNTFASDPIGSKRSSMQRHSQRSLRRKIIEQANEGLQMNQTQLIEFNPNLGDVTRATRVAYVKFMRKKMAADEVSLADDEGAPNGEGEKKPLDASGSKKSITSGGTGGGASMLAAAALRASVKNVDEKSGADGKPGTMGKPTDDKKAGDDKDKQQPPKDSKPSAGGPKPGDQKPTPGAGAPKPQAAGTISKPGESQKKDAPAPPTKPGDTKPAAPKPGESAKPEAAAKKEESSKTEASKPAATNGAAKSAAPSAPSDAKPDSKLKPGAAGAPEATKATNGASKPDEKKSGPEEPKKAAGDSKPGDDAKDKDKKPGDDKDKKPGDDKDKKPADNNDKKPADDKDKKPGDDKDKKPGDDKDKKPSDDKDKKPADDKDKKPAAAPLKPAIKVGQSSAAAGGERGKSTVTGRMISGWL

Target ORF information:

RefSeq Version	NM_057420.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster transient receptor potential (trp), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057420.4

ORF Insert Sequence:

ATGGGCAGCA ATACGGAATC CGATGCCGAG AAGGCGTTGG GGTCTCGCCT GGATTACGAC 
CTGATGATGG CCGAGGAGTA CATCCTCAGC GATGTGGAGA AGAATTTCAT ATTGTCCTGC 
GAGCGGGGTG ACTTGCCAGG TGTCAAGAAG ATCCTCGAGG AGTACCAGGG CACGGACAAG 
TTCAACATTA ACTGCACGGA TCCCATGAAC CGCTCCGCCC TCATTTCGGC CATCGAGAAC 
GAGAACTTCG ACCTGATGGT GATCCTGCTG GAGCATAACA TCGAGGTGGG CGACGCCCTG 
TTGCACGCCA TCTCGGAGGA GTATGTGGAG GCGGTGGAGG AGCTGCTGCA GTGGGAGGAG 
ACCAACCACA AGGAGGGCCA GCCATACAGC TGGGAGGCGG TGGACCGCTC CAAGTCCACC 
TTCACCGTGG ACATCACGCC CCTTATCCTG GCCGCCCACC GAAATAACTA CGAGATACTC 
AAAATCCTCC TGGATCGCGG GGCCACGCTG CCCATGCCGC ACGACGTCAA GTGCGGCTGC 
GATGAGTGTG TGACCTCCCA GATGACGGAC TCCCTGCGCC ACTCGCAGTC GAGGATCAAC 
GCATACCGCG CCCTGTCCGC CAGCTCGCTG ATAGCGCTCA GCTCCCGGGA CCCTGTACTG 
ACCGCCTTCC AATTGTCCTG GGAACTCAAG CGCCTGCAGG CGATGGAATC GGAGTTTCGT 
GCCGAATACA CGGAGATGCG TCAGATGGTG CAGGACTTCG GGACCTCGCT CCTGGATCAC 
GCACGCACAT CCATGGAACT CGAGGTGATG CTCAACTTCA ACCACGAGCC GTCCCACGAC 
ATCTGGTGCC TTGGCCAGCG GCAAACCCTG GAACGACTGA AGCTGGCCAT TCGCTATAAG 
CAAAAAACGT TTGTGGCACA TCCAAATGTC CAACAATTGT TGGCCGCCAT TTGGTACGAC 
GGACTGCCGG GCTTCCGGCG CAAGCAGGCC TCCCAGCAGC TGATGGATGT CGTGAAGCTG 
GGATGCAGCT TCCCCATCTA CAGCTTGAAG TACATCCTGG CCCCGGATTC CGAGGGTGCC 
AAGTTCATGC GCAAGCCCTT TGTCAAGTTC ATCACGCACT CCTGCTCCTA CATGTTCTTC 
CTGATGCTCC TGGGTGCTGC CTCCCTGAGG GTGGTGCAAA TCACCTTTGA ACTCCTCGCA 
TTTCCCTGGA TGCTGACCAT GCTGGAGGAT TGGCGCAAAC ACGAGAGAGG TTCACTACCG 
GGTCCCATTG AACTGGCAAT CATTACCTAC ATAATGGCTC TAATATTTGA GGAACTGAAA 
TCTTTATATT CGGACGGCTT GTTTGAGTAC ATCATGGATC TTTGGAACAT AGTGGACTAC 
ATATCGAACA TGTTCTATGT GACGTGGATT CTTTGTAGGG CCACCGCTTG GGTAATCGTC 
CATCGTGATC TCTGGTTCCG GGGCATAGAT CCTTACTTCC CAAGGGAACA CTGGCATCCG 
TTTGATCCAA TGCTTCTATC AGAGGGCGCC TTTGCTGCCG GAATGGTCTT CTCCTATCTA 
AAGCTCGTCC ACATCTTCTC CATTAATCCC CACCTGGGAC CCTTGCAAGT TTCACTGGGT 
CGCATGATAA TCGACATCAT CAAGTTCTTC TTCATCTACA CACTGGTGTT GTTTGCCTTC 
GGATGTGGTC TCAACCAGTT GCTATGGTAC TACGCTGAGC TGGAGAAGAA CAAGTGCTAT 
CACCTGCATC CCGATGTGGC TGACTTTGAT GACCAGGAGA AGGCTTGTAC CATCTGGCGA 
AGATTTTCCA ACTTATTCGA AACATCACAA TCGCTCTTTT GGGCCTCTTT TGGCCTGGTG 
GACCTGGTCT CCTTCGATCT GGCGGGAATC AAGAGCTTCA CCCGCTTCTG GGCACTGCTA 
ATGTTCGGCT CCTATTCGGT TATCAACATC ATTGTGCTTC TCAACATGCT GATTGCCATG 
ATGTCCAACT CCTACCAAAT CATCTCGGAG CGAGCCGACA CTGAGTGGAA GTTCGCCCGA 
TCCCAGCTGT GGATGAGCTA CTTCGAGGAT GGCGGCACCA TTCCACCGCC CTTCAACCTC 
TGTCCCAACA TGAAGATGTT GAGGAAGACC CTGGGCCGAA AGCGACCGTC ACGAACCAAG 
AGCTTCATGC GAAAGTCCAT GGAACGGGCA CAGACGCTGC ATGACAAAGT GATGAAGCTG 
CTGGTCAGGA GGTACATTAC GGCGGAGCAG AGGCGGCGGG ACGATTACGG CATTACCGAG 
GATGATATCA TTGAGGTGCG CCAGGACATC AGCTCCTTGC GGTTCGAGTT GCTGGAGATT 
TTCACCAACA ATAACTGGGA TGTACCCGAC ATTGAGAAGA AGTCGCAGGG AGTTGCTCGA 
ACCACCAAGG GCAAGGTGAT GGAACGTCGC ATCCTTAAGG ACTTCCAAAT CGGCTTCGTC 
GAGAATCTGA AGCAGGAAAT GAGCGAATCT GAGAGCGGAC GAGATATATT CTCATCGCTG 
GCCAAGGTCA TCGGCAGAAA GAAGACCCAG AAGGGAGACA AGGATTGGAA CGCCATTGCG 
AGGAAGAATA CTTTCGCCTC CGATCCCATT GGCTCCAAGC GCTCCTCCAT GCAACGTCAT 
AGCCAGCGAA GCTTGAGGAG GAAGATCATC GAGCAGGCGA ATGAGGGTCT TCAGATGAAC 
CAGACCCAGT TGATTGAATT CAATCCCAAC TTGGGTGATG TGACGCGTGC CACAAGAGTG 
GCTTATGTCA AGTTCATGCG GAAGAAGATG GCTGCCGACG AGGTTTCCTT GGCCGATGAC 
GAGGGTGCTC CAAATGGCGA AGGCGAAAAG AAGCCACTGG ATGCCTCTGG GTCTAAAAAG 
TCCATAACTA GTGGTGGAAC TGGAGGAGGA GCTTCTATGT TGGCTGCAGC TGCTCTAAGA 
GCATCGGTTA AGAATGTGGA TGAAAAATCC GGAGCCGATG GCAAGCCCGG CACGATGGGC 
AAGCCAACGG ATGACAAGAA AGCAGGTGAT GATAAGGATA AGCAGCAGCC TCCCAAGGAC 
TCCAAGCCGT CAGCAGGTGG TCCCAAGCCC GGGGATCAGA AGCCAACTCC GGGTGCGGGA 
GCTCCAAAGC CCCAAGCAGC TGGCACTATC AGCAAGCCCG GTGAGTCACA AAAGAAGGAC 
GCTCCGGCAC CACCAACCAA ACCTGGAGAC ACCAAGCCTG CTGCGCCGAA GCCTGGAGAA 
TCCGCCAAGC CCGAGGCCGC TGCCAAAAAG GAGGAGTCTT CCAAGACCGA AGCTAGCAAG 
CCGGCAGCCA CAAATGGAGC AGCCAAGAGC GCAGCTCCCT CCGCTCCTTC GGATGCCAAG 
CCGGATTCCA AACTGAAACC TGGAGCAGCT GGAGCACCAG AAGCAACTAA GGCAACCAAT 
GGGGCCTCCA AGCCGGACGA AAAGAAGAGC GGTCCGGAGG AGCCAAAAAA GGCTGCAGGA 
GACTCCAAGC CAGGAGACGA TGCCAAGGAC AAGGATAAGA AACCCGGCGA CGATAAGGAC 
AAGAAACCTG GCGACGACAA AGACAAGAAA CCTGCTGACA ATAATGATAA GAAGCCAGCC 
GATGACAAGG ACAAGAAGCC GGGAGACGAT AAGGACAAGA AGCCGGGTGA CGACAAGGAC 
AAGAAGCCGA GCGATGATAA GGACAAGAAG CCTGCCGATG ACAAGGACAA GAAGCCAGCA 
GCAGCTCCTC TGAAGCCGGC GATCAAGGTG GGTCAGAGCA GTGCCGCAGC TGGCGGAGAA 
CGAGGCAAAT CCACGGTCAC AGGACGCATG ATCTCCGGCT GGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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trp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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