Slbp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slbp
Entrez Gene ID	43448
Full Name	Stem-loop binding protein
Synonyms	CG11886,Dmel\CG11886,SLBP,dSLBP,dSLPB,slbp
General protein information	Preferred Names Stem-loop binding protein Names CG11886 gene product from transcript CG11886-RA CG11886-PA Slbp-PA stem loop binding protein stem-loop-binding protein
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F13-98F13

mRNA and Protein(s)

mRNA	Protein	Name
NM_058132.3	NP_477480.1	Stem-loop binding protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although the C-terminal tail of dSLBP does not contact the RNA, phosphorylation of the tail promotes SLBP conformations competent for RNA binding and thereby appears to reduce the entropic penalty for the association.
Title: Molecular mechanisms for the regulation of histone mRNA stem-loop-binding protein by phosphorylation.

nuclear retention involves Chk2-mediated inactivation of the Drosophila stem loop binding protein (SLBP), the levels of which are specifically depleted in damaged nuclei following Chk2 phosphorylation, an event that contributes to nuclear fallout.
Title: Developmentally regulated elimination of damaged nuclei involves a Chk2-dependent mechanism of mRNA nuclear retention.

The nuclear magnetic resonance and kinetic studies presented here provide a framework for understanding how SLBP recognizes histone mRNA and highlight possible structural roles of phosphorylation and proline isomerization in RNA binding proteins
Title: Interaction of the histone mRNA hairpin with stem-loop binding protein (SLBP) and regulation of the SLBP-RNA complex by phosphorylation and proline isomerization.

Slbp mutants display genomic instability, including loss of heterozygosity (LOH), increased presence of chromosome breaks, tetraploidy, and changes in position effect variegation (PEV).
Title: Loss of the histone pre-mRNA processing factor stem-loop binding protein in Drosophila causes genomic instability and impaired cellular proliferation.

Removal of the phosphoryl group from T230 by either dephosphorylation or mutation results in a 7-fold reduction in the affinity of SLBP for the stem-loop RNA
Title: Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding.

The following Slbp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slbp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06473	NM_058132.3 Latest version!	Drosophila melanogaster Stem-loop binding protein (Slbp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slbp ( NM_058132.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477480.1 Drosophila melanogaster Stem-loop binding protein (Slbp), mRNA.

Price & Availability↑

CloneID	OFa06473
Clone ID Related Accession (Same CDS sequence)	NM_058132.3
Accession Version	NM_058132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Stem-loop binding protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_058132.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCTGCG AGGATCAGCA CATGTCCGTG GAGAACACAC CGCAGAAAGG CTCCGGCAGC 
CTGAATTCCT CAGCATCCTC CATTTCGATC GATGTGAAGC CCACCATGCA GAGCTGGGCC 
CAGGAGGTCC GCGCGGAGTT TGGCCACAGT GACGAGGCCT CCAGTTCCTT GAATAGCAGC 
GCTGCCAGCT GCGGTTCCTT GGCCAAAAAG GAGACAGCCG ACGGTAATCT GGAGTCCAAA 
GATGGCGAGG GCCGGGAGAT GGCATTTGAG TTCCTGGATG GCGTCAACGA GGTAAAGTTC 
GAGCGCCTGG TCAAGGAGGA GAAGCTAAAA ACGCCCTACA AGCGCCGACA CTCGTTCACG 
CCGCCCAGCA ACGAGAACAG TCGCTCCAAC AGTCCAAACA GCAGCAACTC CTCGGCCAAC 
GGAGATGCAG CTGCGCCCAA AGGCGGCAAC AATCCCCATT CGAGGAACTC AAAAAAGTCT 
GGCAACTTCC GCGCCCACAA GGAGGAGAAA CGGGTGCGCC ACAACAGCTA CACGTCCTCC 
ACCTCATCGT CATCCTCCTA CACCGAAGCC GATCCCGCCA TCCTAAGTCG CAGGCAGAAG 
CAAATAGATT ACGGAAAGAA TACTGCCGCC TACGAGCGCT ATGTGGAAAT GGTGCCAAAG 
GACGAGCGGA CTCGCGATCA TCCGCGCACC CCGAACAAGT ATGGAAAGTA CAGCCGCCGC 
GCCTTCGATG GGCTCGTGAA GATCTGGCGC AAAAGCCTCC ACATCTATGA TCCACCTACA 
CAAGCGCGCG ACACGGCCAA GGACAGTAAT TCCGACTCGG ATTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477480.1
CDS	128..958
Translation	MLCEDQHMSVENTPQKGSGSLNSSASSISIDVKPTMQSWAQEVRAEFGHSDEASSSLNSSAASCGSLAKKETADGNLESKDGEGREMAFEFLDGVNEVKFERLVKEEKLKTPYKRRHSFTPPSNENSRSNSPNSSNSSANGDAAAPKGGNNPHSRNSKKSGNFRAHKEEKRVRHNSYTSSTSSSSSYTEADPAILSRRQKQIDYGKNTAAYERYVEMVPKDERTRDHPRTPNKYGKYSRRAFDGLVKIWRKSLHIYDPPTQARDTAKDSNSDSDSD

Target ORF information:

RefSeq Version	NM_058132.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Stem-loop binding protein (Slbp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_058132.3

ORF Insert Sequence:

ATGCTCTGCG AGGATCAGCA CATGTCCGTG GAGAACACAC CGCAGAAAGG CTCCGGCAGC 
CTGAATTCCT CAGCATCCTC CATTTCGATC GATGTGAAGC CCACCATGCA GAGCTGGGCC 
CAGGAGGTCC GCGCGGAGTT TGGCCACAGT GACGAGGCCT CCAGTTCCTT GAATAGCAGC 
GCTGCCAGCT GCGGTTCCTT GGCCAAAAAG GAGACAGCCG ACGGTAATCT GGAGTCCAAA 
GATGGCGAGG GCCGGGAGAT GGCATTTGAG TTCCTGGATG GCGTCAACGA GGTAAAGTTC 
GAGCGCCTGG TCAAGGAGGA GAAGCTAAAA ACGCCCTACA AGCGCCGACA CTCGTTCACG 
CCGCCCAGCA ACGAGAACAG TCGCTCCAAC AGTCCAAACA GCAGCAACTC CTCGGCCAAC 
GGAGATGCAG CTGCGCCCAA AGGCGGCAAC AATCCCCATT CGAGGAACTC AAAAAAGTCT 
GGCAACTTCC GCGCCCACAA GGAGGAGAAA CGGGTGCGCC ACAACAGCTA CACGTCCTCC 
ACCTCATCGT CATCCTCCTA CACCGAAGCC GATCCCGCCA TCCTAAGTCG CAGGCAGAAG 
CAAATAGATT ACGGAAAGAA TACTGCCGCC TACGAGCGCT ATGTGGAAAT GGTGCCAAAG 
GACGAGCGGA CTCGCGATCA TCCGCGCACC CCGAACAAGT ATGGAAAGTA CAGCCGCCGC 
GCCTTCGATG GGCTCGTGAA GATCTGGCGC AAAAGCCTCC ACATCTATGA TCCACCTACA 
CAAGCGCGCG ACACGGCCAA GGACAGTAAT TCCGACTCGG ATTCTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Slbp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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