spg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	spg
Entrez Gene ID	43404
Full Name	sponge
Synonyms	CG11754,CG14530,CG31048,Dm Dock4,Dmel\CG31048,Spg,mat(3)6,mat3(6)
General protein information	Preferred Names sponge Names CG31048 gene product from transcript CG31048-RD CG31048-PD CG31048-PE CG31048-PF CG31048-PG CG31048-PH Dock4 early-D spg-PD spg-PE spg-PF spg-PG spg-PH
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98E6-98F1\|3-95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_170379.5	NP_733258.4	sponge, isoform E
NM_001144646.2	NP_001138118.2	sponge, isoform D
NM_001276118.1	NP_001263047.1	sponge, isoform F
NM_001276120.1	NP_001263049.1	sponge, isoform H
NM_001276119.1	NP_001263048.1	sponge, isoform G

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DOCK3	XP_516488.4
Xenopus tropicalis (tropical clawed frog)	dock3	XP_004914390.1
	DOCK3	XP_001089458.2
Rattus norvegicus (Norway rat)	Dock3	XP_006243927.1
Homo sapiens (human)	DOCK3	NP_004938.1
Canis lupus familiaris (dog)	DOCK3	XP_533813.4
Danio rerio (zebrafish)	dock3	XP_003201183.2
Drosophila melanogaster (fruit fly)	spg	NP_001263047.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004507	XP_003436830.1
Bos taurus (cattle)	LOC524692	XP_002697118.2
Mus musculus (house mouse)	Dock3	NP_700462.2
Gallus gallus (chicken)	DOCK3	XP_414263.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Spg plays a critical role in air sac primordium development.
Title: The Drosophila DOCK family protein Sponge is required for development of the air sac primordium.

Spg positively regulates JNK pathway that is required for thorax development and the regulation is mediated by interaction with Rac1.
Title: Drosophila DOCK family protein sponge regulates the JNK pathway during thorax development.

Knockdown of spg reduced the level of activated ERK signal and induces abnormal eye morphology.
Title: The Drosophila DOCK family protein sponge is involved in differentiation of R7 photoreceptor cells.

The following spg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06422	NM_170379.5 Latest version!	Drosophila melanogaster sponge (spg), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08930	NM_001144646.2 Latest version!	Drosophila melanogaster sponge (spg), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08930	NM_001276120.1 Latest version!	Drosophila melanogaster sponge (spg), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08931	NM_001276119.1 Latest version!	Drosophila melanogaster sponge (spg), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08931	NM_001276118.1 Latest version!	Drosophila melanogaster sponge (spg), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spg ( NM_170379.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733258.4 Drosophila melanogaster sponge (spg), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa06422
Clone ID Related Accession (Same CDS sequence)	NM_170379.5
Accession Version	NM_170379.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sponge, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_170379.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATT TTGCTGGAGA AAGTCCAAGT GGAGAACCCC 
AACTGGAAGG AGGCTGGCAT CGCTTTCATA GCCTCAGTCA CCAGACTTTT GGAGCGCCTT 
CTGGACTATC GTAGCGTAAT GCAGGGCGAG GAAAATCGGG ATAAGCGCAT GACCTGCACA 
GTCAATCTCC TTAATTTCTA TAAAAACGAA ATCAATCGAA AGGAGATGTA TCTGAGATAC 
ATTTACAAGC TGCACAACCT GCACCTGCAG GCAGAGAATT ACACAGAGGC TGGCTTCACT 
CTGAAACTGT ATGCATCCAT GCTGAGTTGG GATCGTGAGA CCCAGAGCTT TGCCCCCTTC 
GATAATAGTG GTCAGCCAGA GTGGCAGCGC AAGGAGCGTC TATACCACGA GATCCTAAAA 
TACTTCGACA AGGGAAAATG TTGGGAAAAG GGCATTCCAC TGTGCAAGGA GCTGGCTCAT 
TTATACGAAA CGCGGCGTTT TGACTATAAC AAGTTGAGTG AAATACTCAT TCAGGAGGCC 
AAGTTCTTCC AGAATATCCT AACTCAGTTG CGACCGGAGC CGGAATACTT CCGTGTGGGA 
TTCTACGGAA TGGGTCTGCC ACTTTTTGTG CGGAACAAGC AATTTGTGTA CCGTGGACTG 
GAGTACGAGA GAATAGGAGC CTTCACACAG CGTCTGCAAA CGGAGTTTCC TAGTGCTCAG 
ATCTTGGGCA ACAACAGTCC GCCGGACAAC GCCATTCTGA ATGCTCCGGA TCAGTACATA 
CAGATCAGCA ATGTAAGACC TGTTGGGGAT GCACAGGCCT TGAAGACCGC AATGGTTCCT 
GTGCCGGAGA AAATAGCTCG TTTCTACGAG GTCAACGATG TAACCCGATT CATCTACGAT 
CGCCCCATGT ACAAGGGAAC AGTTGACAAG GACAATGAGT TCAAGTCGTT GTGGATAGAA 
CGCACTATTC TAGAGATAGC TAGTCCACTG CCAGGTATTC TACGCTGGTA TGAGGTCAAA 
CAGAAGACGA TGCAGGAGTT AACGCCGGTA GAGTACGCTT GCGAGATTAT CAGCAACGCT 
GGCAAGGAAT TATCCGAGCT GATTGTGCAA TATAAGCGCG ATCCCAAGAG GAATATTAAT 
CCCTTCTCCA TGCGTCTGCA GGGCACAATC GATGCTAATG TCATGGGGGG TATAAGCAAG 
TACCAGGAGG CTTTCTTCTC CGAGCAGTTC CTCAAGTCGC CGCAAGGTGC TGGACAGCAG 
GCAAATGTGC AGCGGCTGAA GGTTCTGATA CTGGAGCAGA TTCAGATATT GGAGCAGGCC 
CTCGAACTGC ACGGTCAGCT AGCGCCCAGT GGTGTCCAGC CTCTGCACAA TCGTCTTTTG 
GAACGCTTTT CCCAGCTTAA GCAAAGTTTA TCCGGCATGG GCAGACTGAA ACGTCAGCAC 
TCCGAGAGCA TTGTAAATAC TCCCTTGCCA CCGTTGCCTA CAGAGCAGCG AGTAGCCCCG 
GCGACAGCTT CCTCAAGCTT GAATGCGAAT GCCCACTCCA ACTATGTATA TGATCTAGAT 
GAGATCTACA CGAGACCGGG AGACACTGTA CGACCCGTGG ACCCACTCAG CTCCTATCAA 
ACACTGAGCA AGGAGAGTCT TAGTATTCCT CTGGAGGATA CTGCTGCTCC TCCAGTACCA 
AATCGTCCTC GCTCGCAGAA CTTTATTGGA AACGGATCCA TGGACAGCCC GGAGGTTCCG 
CCGAAACGAC AGCCTACATT GAATTCTCCC AATGCTCCTC CGCTGCCTCC GCGGGGTATT 
ACACCGGACA AGAGGGCGTC AAATCCGTTG ATTTTCAATG ACTTCGGCTC CGGAGATGCC 
GTGGGACGTC GGCATTCTCA GCAGCAGCAG CAGCATGGCC AGAAGTATTC CGTTGTTGAT 
ATTAGCTACG ATGATCCAGA GGCAGATCAG CAGCCGCATT CGCTGCCGCC AAACTTCCAG 
GACGGAAGCG GTGGACACCT CAGCGTCTGT TTTGCCCCGA ATGATTTCCG GGACTCCGGC 
ATATCGACGA CTAGTTCTAG GGAACTGAAC CACATGAATC TTAACAACCT GAGCGAAGAC 
TCATCGTCGA TCTCGACCAT TACAGTGCAG CATCGAGAAC ACTGTCGCAT CAGCAGCAAT 
GGCAGTTTGG ACTATAATGC CGCAGCATCA ATGAACATTA CACAACGAGA GAGTTCGACG 
AGTTCGTTTG ATGTGGAGGA CTTTCCAGTG CCGCCGCCGC CCATTCCACC CAAATCCTTG 
GCAGTGTCAA GCAACGGTGT CAACTTTTCA CTCGACGAGG CCCACTCACC GCATCCCAGC 
ACACAAGCGA ACACCACACA ACTCAATCAC ATTCAGAACC CAAATCAGAA TCATTCCACA 
AACAGTCACC ATCATCTTCA GCAGCAGCAG AATGGAGATG GCGATGGTTA TAGCACGCTG 
CAGCACCCGA TGAACGGGCT GGGGCTGCCT ACCCTTCCAC CACCACCTTC TGCTGTTGAA 
TCGCCAATGC CGGCATCTGC AATAGCCTCA TCCACATCTA GTCATCAGCA CGATGGCGGC 
ACTTTTTGAG TTACAATATG TCGCTGCCAT CATCCACCCG ATCAGCCACA TCAGCCACAC 
AAGCCATCCT CATCCACGTC AACCTCGGAC AGCACGGCCA CGGATACGGC TTCGGAATCC 
ACGGAATCCA GTTCGTCTAC CCATTCGATG ACGCCGCCAC CGCCTCCCCC ACCGCCGCCA 
TCGTCCTTCA CGCACTTGCA CCATCAGATG GCAGCAACAA CTCACTATTG CCAGGATTGT 
CTGTCCTCGC CATCGCCACC ACCCACAATC ATCACAGAGC AGGCACAAGT GCATCCCACT 
CCGGGCTCGA CCTCAGCACA GCCCCACATC GAGCACTGCT CTTGTGGATT GAGTTTCTCC 
GTGGTTCAGG ATCAGCAGGA GCAGTTTCCC AGGCTACTCT CCACCAGATT TTCAACGCTG 
GAGCGGCAGA CCGATGGTTT TCAGGTGGAG CAGGTGTCTG GCTGCCAGCA TCATCATCAT 
CACCAGCATA GCCCGTTGTA TCATCTTCCT CAAAGCAGTC AGGATGCACA TGCTCAGACG 
GATTTTTCCT ACGCGCAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733258.4
CDS	129..6692
Translation	MWIPSNNKYGVAIHNWHGDVRFGLALDVGDSVDIVEECKHWYRGSCPRKSRAVGLFPKTYIHIKDLSKIDPVVAECTQVLREWSEIWKRLYVDREAYKFQTLRKVMFSILESRRELLSATMTQDQTLELQMVVVSKIDWGNRKLGLDLVPREGPLAVDPHGIGIVKLYNVHVSSADNAKASSSRGTLRRKTQRKILTHHLYFCMRDFGHRIGGDDAEVYFFLYDGSHMRPLSERFLVKISKDGFSNYIERLHSNCTVFTDLGAADLNEELHLVAVVMRVGKIIQSDSIKKIEKSGTLGHGPTFRRPFGVGVLSLGDIAHFDSSLEQSSDEREYNFKLFQCEEKDFHLLPELIIKKSSGKYSPIQASQGTQGIVVSLKLLHGGLGQARQEQPMLFQGSTITRKMGFPDVIMPGDVRNDLFLNLERGEFERGGKNTGKNILVTVVVLDVAGNVLTDCLWGASGMESQPQYRSMILYHQNAPSWNEMLRLSVPIDKFSTAHVRFEFRHCSTRDKTEPKLFAFSFARLMDTGGATLGDGLHELYVYKCEDPAKLQVANYLRLQCRPRDAQAKMDCGGCFSRSSKEVFVLRSLLCSTKLTQNADLLSLLQWRTYPETIQDSLTGVLRLNDEELVKFLQDVLDALFAMFSNEEGNSTQHSGLVFHVLVSIFSLLQSNKFQHFRPVMNEYIENHFAAALVYKGLITSVEHMAVFMTKAEHPDPFQKCFGSLEYIFKLLIQSRKLFARATGGQYEDSFKRDLHSLFTALNGMLAVPSYDVIIPTQEALLNSTGVVLEQLKDTLPAPELGMLARNMLDAIPRGAPIRLIQAKLHAVKDLVSGELFHEDDSRTVVLSVACKHLRMHLSRRDELRLCAEILSEILSQLYDLQKEQREKVTNTLQHDLDSLCKNILGILIRTISIIMEGSNAVLPQLVSCLLGLLQLLDETHYKRYWDELSPNKDPRDLKEFLSKSLLVYEELLTQDWHVFPNDWLVMKLAANDVLRMSLEEFAKPLVYRFLGPQAFDSQLWWSYFSLAVAFLTQPSLQLEQYREPKRLKILHSHGDMRVLMGFQILSMWSQLGEQKLHFIPSMVGPFLEVTLVPEPALRKATLSVFYDMMQCEQAARGSFRLVESELIDKLDLLISENKGDDEYRELFSTILLEKVQVENPNWKEAGIAFIASVTRLLERLLDYRSVMQGEENRDKRMTCTVNLLNFYKNEINRKEMYLRYIYKLHNLHLQAENYTEAGFTLKLYASMLSWDRETQSFAPFDNSGQPEWQRKERLYHEILKYFDKGKCWEKGIPLCKELAHLYETRRFDYNKLSEILIQEAKFFQNILTQLRPEPEYFRVGFYGMGLPLFVRNKQFVYRGLEYERIGAFTQRLQTEFPSAQILGNNSPPDNAILNAPDQYIQISNVRPVGDAQALKTAMVPVPEKIARFYEVNDVTRFIYDRPMYKGTVDKDNEFKSLWIERTILEIASPLPGILRWYEVKQKTMQELTPVEYACEIISNAGKELSELIVQYKRDPKRNINPFSMRLQGTIDANVMGGISKYQEAFFSEQFLKSPQGAGQQANVQRLKVLILEQIQILEQALELHGQLAPSGVQPLHNRLLERFSQLKQSLSGMGRLKRQHSESIVNTPLPPLPTEQRVAPATASSSLNANAHSNYVYDLDEIYTRPGDTVRPVDPLSSYQTLSKESLSIPLEDTAAPPVPNRPRSQNFIGNGSMDSPEVPPKRQPTLNSPNAPPLPPRGITPDKRASNPLIFNDFGSGDAVGRRHSQQQQQHGQKYSVVDISYDDPEADQQPHSLPPNFQDGSGGHLSVCFAPNDFRDSGISTTSSRELNHMNLNNLSEDSSSISTITVQHREHCRISSNGSLDYNAAASMNITQRESSTSSFDVEDFPVPPPPIPPKSLAVSSNGVNFSLDEAHSPHPSTQANTTQLNHIQNPNQNHSTNSHHHLQQQQNGDGDGYSTLQHPMNGLGLPTLPPPPSAVESPMPASAIASSTSSHQHDGGTF*VTICRCHHPPDQPHQPHKPSSSTSTSDSTATDTASESTESSSSTHSMTPPPPPPPPPSSFTHLHHQMAATTHYCQDCLSSPSPPPTIITEQAQVHPTPGSTSAQPHIEHCSCGLSFSVVQDQQEQFPRLLSTRFSTLERQTDGFQVEQVSGCQHHHHHQHSPLYHLPQSSQDAHAQTDFSYAQR

Target ORF information:

RefSeq Version	NM_170379.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sponge (spg), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170379.5

ORF Insert Sequence:

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATT TTGCTGGAGA AAGTCCAAGT GGAGAACCCC 
AACTGGAAGG AGGCTGGCAT CGCTTTCATA GCCTCAGTCA CCAGACTTTT GGAGCGCCTT 
CTGGACTATC GTAGCGTAAT GCAGGGCGAG GAAAATCGGG ATAAGCGCAT GACCTGCACA 
GTCAATCTCC TTAATTTCTA TAAAAACGAA ATCAATCGAA AGGAGATGTA TCTGAGATAC 
ATTTACAAGC TGCACAACCT GCACCTGCAG GCAGAGAATT ACACAGAGGC TGGCTTCACT 
CTGAAACTGT ATGCATCCAT GCTGAGTTGG GATCGTGAGA CCCAGAGCTT TGCCCCCTTC 
GATAATAGTG GTCAGCCAGA GTGGCAGCGC AAGGAGCGTC TATACCACGA GATCCTAAAA 
TACTTCGACA AGGGAAAATG TTGGGAAAAG GGCATTCCAC TGTGCAAGGA GCTGGCTCAT 
TTATACGAAA CGCGGCGTTT TGACTATAAC AAGTTGAGTG AAATACTCAT TCAGGAGGCC 
AAGTTCTTCC AGAATATCCT AACTCAGTTG CGACCGGAGC CGGAATACTT CCGTGTGGGA 
TTCTACGGAA TGGGTCTGCC ACTTTTTGTG CGGAACAAGC AATTTGTGTA CCGTGGACTG 
GAGTACGAGA GAATAGGAGC CTTCACACAG CGTCTGCAAA CGGAGTTTCC TAGTGCTCAG 
ATCTTGGGCA ACAACAGTCC GCCGGACAAC GCCATTCTGA ATGCTCCGGA TCAGTACATA 
CAGATCAGCA ATGTAAGACC TGTTGGGGAT GCACAGGCCT TGAAGACCGC AATGGTTCCT 
GTGCCGGAGA AAATAGCTCG TTTCTACGAG GTCAACGATG TAACCCGATT CATCTACGAT 
CGCCCCATGT ACAAGGGAAC AGTTGACAAG GACAATGAGT TCAAGTCGTT GTGGATAGAA 
CGCACTATTC TAGAGATAGC TAGTCCACTG CCAGGTATTC TACGCTGGTA TGAGGTCAAA 
CAGAAGACGA TGCAGGAGTT AACGCCGGTA GAGTACGCTT GCGAGATTAT CAGCAACGCT 
GGCAAGGAAT TATCCGAGCT GATTGTGCAA TATAAGCGCG ATCCCAAGAG GAATATTAAT 
CCCTTCTCCA TGCGTCTGCA GGGCACAATC GATGCTAATG TCATGGGGGG TATAAGCAAG 
TACCAGGAGG CTTTCTTCTC CGAGCAGTTC CTCAAGTCGC CGCAAGGTGC TGGACAGCAG 
GCAAATGTGC AGCGGCTGAA GGTTCTGATA CTGGAGCAGA TTCAGATATT GGAGCAGGCC 
CTCGAACTGC ACGGTCAGCT AGCGCCCAGT GGTGTCCAGC CTCTGCACAA TCGTCTTTTG 
GAACGCTTTT CCCAGCTTAA GCAAAGTTTA TCCGGCATGG GCAGACTGAA ACGTCAGCAC 
TCCGAGAGCA TTGTAAATAC TCCCTTGCCA CCGTTGCCTA CAGAGCAGCG AGTAGCCCCG 
GCGACAGCTT CCTCAAGCTT GAATGCGAAT GCCCACTCCA ACTATGTATA TGATCTAGAT 
GAGATCTACA CGAGACCGGG AGACACTGTA CGACCCGTGG ACCCACTCAG CTCCTATCAA 
ACACTGAGCA AGGAGAGTCT TAGTATTCCT CTGGAGGATA CTGCTGCTCC TCCAGTACCA 
AATCGTCCTC GCTCGCAGAA CTTTATTGGA AACGGATCCA TGGACAGCCC GGAGGTTCCG 
CCGAAACGAC AGCCTACATT GAATTCTCCC AATGCTCCTC CGCTGCCTCC GCGGGGTATT 
ACACCGGACA AGAGGGCGTC AAATCCGTTG ATTTTCAATG ACTTCGGCTC CGGAGATGCC 
GTGGGACGTC GGCATTCTCA GCAGCAGCAG CAGCATGGCC AGAAGTATTC CGTTGTTGAT 
ATTAGCTACG ATGATCCAGA GGCAGATCAG CAGCCGCATT CGCTGCCGCC AAACTTCCAG 
GACGGAAGCG GTGGACACCT CAGCGTCTGT TTTGCCCCGA ATGATTTCCG GGACTCCGGC 
ATATCGACGA CTAGTTCTAG GGAACTGAAC CACATGAATC TTAACAACCT GAGCGAAGAC 
TCATCGTCGA TCTCGACCAT TACAGTGCAG CATCGAGAAC ACTGTCGCAT CAGCAGCAAT 
GGCAGTTTGG ACTATAATGC CGCAGCATCA ATGAACATTA CACAACGAGA GAGTTCGACG 
AGTTCGTTTG ATGTGGAGGA CTTTCCAGTG CCGCCGCCGC CCATTCCACC CAAATCCTTG 
GCAGTGTCAA GCAACGGTGT CAACTTTTCA CTCGACGAGG CCCACTCACC GCATCCCAGC 
ACACAAGCGA ACACCACACA ACTCAATCAC ATTCAGAACC CAAATCAGAA TCATTCCACA 
AACAGTCACC ATCATCTTCA GCAGCAGCAG AATGGAGATG GCGATGGTTA TAGCACGCTG 
CAGCACCCGA TGAACGGGCT GGGGCTGCCT ACCCTTCCAC CACCACCTTC TGCTGTTGAA 
TCGCCAATGC CGGCATCTGC AATAGCCTCA TCCACATCTA GTCATCAGCA CGATGGCGGC 
ACTTTTTGAG TTACAATATG TCGCTGCCAT CATCCACCCG ATCAGCCACA TCAGCCACAC 
AAGCCATCCT CATCCACGTC AACCTCGGAC AGCACGGCCA CGGATACGGC TTCGGAATCC 
ACGGAATCCA GTTCGTCTAC CCATTCGATG ACGCCGCCAC CGCCTCCCCC ACCGCCGCCA 
TCGTCCTTCA CGCACTTGCA CCATCAGATG GCAGCAACAA CTCACTATTG CCAGGATTGT 
CTGTCCTCGC CATCGCCACC ACCCACAATC ATCACAGAGC AGGCACAAGT GCATCCCACT 
CCGGGCTCGA CCTCAGCACA GCCCCACATC GAGCACTGCT CTTGTGGATT GAGTTTCTCC 
GTGGTTCAGG ATCAGCAGGA GCAGTTTCCC AGGCTACTCT CCACCAGATT TTCAACGCTG 
GAGCGGCAGA CCGATGGTTT TCAGGTGGAG CAGGTGTCTG GCTGCCAGCA TCATCATCAT 
CACCAGCATA GCCCGTTGTA TCATCTTCCT CAAAGCAGTC AGGATGCACA TGCTCAGACG 
GATTTTTCCT ACGCGCAGCG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg ( NM_001144646.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138118.2 Drosophila melanogaster sponge (spg), transcript variant D, mRNA.

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CloneID	OFa08930
Clone ID Related Accession (Same CDS sequence)	NM_001144646.2 , NM_001276120.1
Accession Version	NM_001144646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sponge, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144646.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATT TTGCTGGAGA AAGTCCAAGT GGAGAACCCC 
AACTGGAAGG AGGCTGGCAT CGCTTTCATA GCCTCAGTCA CCAGACTTTT GGAGCGCCTT 
CTGGACTATC GTAGCGTAAT GCAGGGCGAG GAAAATCGGG ATAAGCGCAT GACCTGCACA 
GTCAATCTCC TTAATTTCTA TAAAAACGAA ATCAATCGAA AGGAGATGTA TCTGAGATAC 
ATTTACAAGC TGCACAACCT GCACCTGCAG GCAGAGAATT ACACAGAGGC TGGCTTCACT 
CTGAAACTGT ATGCATCCAT GCTGAGTTGG GATCGTGAGA CCCAGAGCTT TGCCCCCTTC 
GATAATAGTG GTCAGCCAGA GTGGCAGCGC AAGGAGCGTC TATACCACGA GATCCTAAAA 
TACTTCGACA AGGGAAAATG TTGGGAAAAG GGCATTCCAC TGTGCAAGGA GCTGGCTCAT 
TTATACGAAA CGCGGCGTTT TGACTATAAC AAGTTGAGTG AAATACTCAT TCAGGAGGCC 
AAGTTCTTCC AGAATATCCT AACTCAGTTG CGACCGGAGC CGGAATACTT CCGTGTGGGA 
TTCTACGGAA TGGGTCTGCC ACTTTTTGTG CGGAACAAGC AATTTGTGTA CCGTGGACTG 
GAGTACGAGA GAATAGGAGC CTTCACACAG CGTCTGCAAA CGGAGTTTCC TAGTGCTCAG 
ATCTTGGGCA ACAACAGTCC GCCGGACAAC GCCATTCTGA ATGCTCCGGA TCAGTACATA 
CAGATCAGCA ATGTAAGACC TGTTGGGGAT GCACAGGCCT TGAAGACCGC AATGGTTCCT 
GTGCCGGAGA AAATAGCTCG TTTCTACGAG GTCAACGATG TAACCCGATT CATCTACGAT 
CGCCCCATGT ACAAGGGAAC AGTTGACAAG GACAATGAGT TCAAGTCGTT GTGGATAGAA 
CGCACTATTC TAGAGATAGC TAGTCCACTG CCAGGTATTC TACGCTGGTA TGAGGTCAAA 
CAGAAGACGA TGCAGGAGTT AACGCCGGTA GAGTACGCTT GCGAGATTAT CAGCAACGCT 
GGCAAGGAAT TATCCGAGCT GATTGTGCAA TATAAGCGCG ATCCCAAGAG GAATATTAAT 
CCCTTCTCCA TGCGTCTGCA GGGCACAATC GATGCTAATG TCATGGGGGG TATAAGCAAG 
TACCAGGAGG CTTTCTTCTC CGAGCAGTTC CTCAAGTCGC CGCAAGGTGC TGGACAGCAG 
GCAAATGTGC AGCGGCTGAA GGTTCTGATA CTGGAGCAGA TTCAGATATT GGAGCAGGCC 
CTCGAACTGC ACGGTCAGCT AGCGCCCAGT GGTGTCCAGC CTCTGCACAA TCGTCTTTTG 
GAACGCTTTT CCCAGCTTAA GCAAAGTTTA TCCGGCATGG GCAGACTGAA ACGTCAGCAC 
TCCGAGAGCA TTGTAAATAC TCCCTTGCCA CCGTTGCCTA CAGAGCAGCG AGTAGCCCCG 
GCGACAGCTT CCTCAAGCTT GAATGCGAAT GCCCACTCCA ACTATGTATA TGATCTAGAT 
GAGATCTACA CGAGACCGGG AGACACTGTA CGACCCGTGG ACCCACTCAG CTCCTATCAA 
ACACTGAGCA AGGAGAGTCT TAGTATTCCT CTGGAGGATA CTGCTGCTCC TCCAGTACCA 
AATCGTCCTC GCTCGCAGAA CTTTATTGGA AACGGATCCA TGGACAGCCC GGAGGTTCCG 
CCGAAACGAC AGCCTACATT GAATTCTCCC AATGCTCCTC CGCTGCCTCC GCGGGGTATT 
ACACCGGACA AGAGGGCGTC AAATCCGTTG ATTTTCAATG ACTTCGGCTC CGGAGATGCC 
GTGGGACGTC GGCATTCTCA GCAGCAGCAG CAGCATGGCC AGAAGTATTC CGTTGTTGAT 
ATTAGCTACG ATGATCCAGA GGCAGATCAG CAGCCGCATT CGCTGCCGCC AAACTTCCAG 
GACGGAAGCG GTGGACACCT CAGCGTCTGT TTTGCCCCGA ATGATTTCCG GGACTCCGGC 
ATATCGACGA CTAGTTCTAG GGAACTGAAC CACATGAATC TTAACAACCT GAGCGAAGAC 
TCATCGTCGA TCTCGACCAT TACAGTGCAG CATCGAGAAC ACTGTCGCAT CAGCAGCAAT 
GGCAGTTTGG ACTATAATGC CGCAGCATCA ATGAACATTA CACAACGAGA GAGTTCGACG 
AGTTCGTTTG ATGTGGAGGA CTTTCCAGTG CCGCCGCCGC CCATTCCACC CAAATCCTTG 
GCAGTGTCAA GCAACGGTGT CAACTTTTCA CTCGACGAGG CCCACTCACC GCATCCCAGC 
ACACAAGCGA ACACCACACA ACTCAATCAC ATTCAGAACC CAAATCAGAA TCATTCCACA 
AACAGTCACC ATCATCTTCA GCAGCAGCAG AATGGAGATG GCGATGGTTA TAGCACGCTG 
CAGCACCCGA TGAACGGGCT GGGGCTGCCT ACCCTTCCAC CACCACCTTC TGCTGTTGAA 
TCGCCAATGC CGGCATCTGC AATAGCCTCA TCCACATCTA GTCATCAGCA CGATGGCGGC 
ACTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138118.2
CDS	159..6167
Translation	MWIPSNNKYGVAIHNWHGDVRFGLALDVGDSVDIVEECKHWYRGSCPRKSRAVGLFPKTYIHIKDLSKIDPVVAECTQVLREWSEIWKRLYVDREAYKFQTLRKVMFSILESRRELLSATMTQDQTLELQMVVVSKIDWGNRKLGLDLVPREGPLAVDPHGIGIVKLYNVHVSSADNAKASSSRGTLRRKTQRKILTHHLYFCMRDFGHRIGGDDAEVYFFLYDGSHMRPLSERFLVKISKDGFSNYIERLHSNCTVFTDLGAADLNEELHLVAVVMRVGKIIQSDSIKKIEKSGTLGHGPTFRRPFGVGVLSLGDIAHFDSSLEQSSDEREYNFKLFQCEEKDFHLLPELIIKKSSGKYSPIQASQGTQGIVVSLKLLHGGLGQARQEQPMLFQGSTITRKMGFPDVIMPGDVRNDLFLNLERGEFERGGKNTGKNILVTVVVLDVAGNVLTDCLWGASGMESQPQYRSMILYHQNAPSWNEMLRLSVPIDKFSTAHVRFEFRHCSTRDKTEPKLFAFSFARLMDTGGATLGDGLHELYVYKCEDPAKLQVANYLRLQCRPRDAQAKMDCGGCFSRSSKEVFVLRSLLCSTKLTQNADLLSLLQWRTYPETIQDSLTGVLRLNDEELVKFLQDVLDALFAMFSNEEGNSTQHSGLVFHVLVSIFSLLQSNKFQHFRPVMNEYIENHFAAALVYKGLITSVEHMAVFMTKAEHPDPFQKCFGSLEYIFKLLIQSRKLFARATGGQYEDSFKRDLHSLFTALNGMLAVPSYDVIIPTQEALLNSTGVVLEQLKDTLPAPELGMLARNMLDAIPRGAPIRLIQAKLHAVKDLVSGELFHEDDSRTVVLSVACKHLRMHLSRRDELRLCAEILSEILSQLYDLQKEQREKVTNTLQHDLDSLCKNILGILIRTISIIMEGSNAVLPQLVSCLLGLLQLLDETHYKRYWDELSPNKDPRDLKEFLSKSLLVYEELLTQDWHVFPNDWLVMKLAANDVLRMSLEEFAKPLVYRFLGPQAFDSQLWWSYFSLAVAFLTQPSLQLEQYREPKRLKILHSHGDMRVLMGFQILSMWSQLGEQKLHFIPSMVGPFLEVTLVPEPALRKATLSVFYDMMQCEQAARGSFRLVESELIDKLDLLISENKGDDEYRELFSTILLEKVQVENPNWKEAGIAFIASVTRLLERLLDYRSVMQGEENRDKRMTCTVNLLNFYKNEINRKEMYLRYIYKLHNLHLQAENYTEAGFTLKLYASMLSWDRETQSFAPFDNSGQPEWQRKERLYHEILKYFDKGKCWEKGIPLCKELAHLYETRRFDYNKLSEILIQEAKFFQNILTQLRPEPEYFRVGFYGMGLPLFVRNKQFVYRGLEYERIGAFTQRLQTEFPSAQILGNNSPPDNAILNAPDQYIQISNVRPVGDAQALKTAMVPVPEKIARFYEVNDVTRFIYDRPMYKGTVDKDNEFKSLWIERTILEIASPLPGILRWYEVKQKTMQELTPVEYACEIISNAGKELSELIVQYKRDPKRNINPFSMRLQGTIDANVMGGISKYQEAFFSEQFLKSPQGAGQQANVQRLKVLILEQIQILEQALELHGQLAPSGVQPLHNRLLERFSQLKQSLSGMGRLKRQHSESIVNTPLPPLPTEQRVAPATASSSLNANAHSNYVYDLDEIYTRPGDTVRPVDPLSSYQTLSKESLSIPLEDTAAPPVPNRPRSQNFIGNGSMDSPEVPPKRQPTLNSPNAPPLPPRGITPDKRASNPLIFNDFGSGDAVGRRHSQQQQQHGQKYSVVDISYDDPEADQQPHSLPPNFQDGSGGHLSVCFAPNDFRDSGISTTSSRELNHMNLNNLSEDSSSISTITVQHREHCRISSNGSLDYNAAASMNITQRESSTSSFDVEDFPVPPPPIPPKSLAVSSNGVNFSLDEAHSPHPSTQANTTQLNHIQNPNQNHSTNSHHHLQQQQNGDGDGYSTLQHPMNGLGLPTLPPPPSAVESPMPASAIASSTSSHQHDGGTF

Target ORF information:

RefSeq Version	NM_001144646.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sponge (spg), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144646.2

ORF Insert Sequence:

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATT TTGCTGGAGA AAGTCCAAGT GGAGAACCCC 
AACTGGAAGG AGGCTGGCAT CGCTTTCATA GCCTCAGTCA CCAGACTTTT GGAGCGCCTT 
CTGGACTATC GTAGCGTAAT GCAGGGCGAG GAAAATCGGG ATAAGCGCAT GACCTGCACA 
GTCAATCTCC TTAATTTCTA TAAAAACGAA ATCAATCGAA AGGAGATGTA TCTGAGATAC 
ATTTACAAGC TGCACAACCT GCACCTGCAG GCAGAGAATT ACACAGAGGC TGGCTTCACT 
CTGAAACTGT ATGCATCCAT GCTGAGTTGG GATCGTGAGA CCCAGAGCTT TGCCCCCTTC 
GATAATAGTG GTCAGCCAGA GTGGCAGCGC AAGGAGCGTC TATACCACGA GATCCTAAAA 
TACTTCGACA AGGGAAAATG TTGGGAAAAG GGCATTCCAC TGTGCAAGGA GCTGGCTCAT 
TTATACGAAA CGCGGCGTTT TGACTATAAC AAGTTGAGTG AAATACTCAT TCAGGAGGCC 
AAGTTCTTCC AGAATATCCT AACTCAGTTG CGACCGGAGC CGGAATACTT CCGTGTGGGA 
TTCTACGGAA TGGGTCTGCC ACTTTTTGTG CGGAACAAGC AATTTGTGTA CCGTGGACTG 
GAGTACGAGA GAATAGGAGC CTTCACACAG CGTCTGCAAA CGGAGTTTCC TAGTGCTCAG 
ATCTTGGGCA ACAACAGTCC GCCGGACAAC GCCATTCTGA ATGCTCCGGA TCAGTACATA 
CAGATCAGCA ATGTAAGACC TGTTGGGGAT GCACAGGCCT TGAAGACCGC AATGGTTCCT 
GTGCCGGAGA AAATAGCTCG TTTCTACGAG GTCAACGATG TAACCCGATT CATCTACGAT 
CGCCCCATGT ACAAGGGAAC AGTTGACAAG GACAATGAGT TCAAGTCGTT GTGGATAGAA 
CGCACTATTC TAGAGATAGC TAGTCCACTG CCAGGTATTC TACGCTGGTA TGAGGTCAAA 
CAGAAGACGA TGCAGGAGTT AACGCCGGTA GAGTACGCTT GCGAGATTAT CAGCAACGCT 
GGCAAGGAAT TATCCGAGCT GATTGTGCAA TATAAGCGCG ATCCCAAGAG GAATATTAAT 
CCCTTCTCCA TGCGTCTGCA GGGCACAATC GATGCTAATG TCATGGGGGG TATAAGCAAG 
TACCAGGAGG CTTTCTTCTC CGAGCAGTTC CTCAAGTCGC CGCAAGGTGC TGGACAGCAG 
GCAAATGTGC AGCGGCTGAA GGTTCTGATA CTGGAGCAGA TTCAGATATT GGAGCAGGCC 
CTCGAACTGC ACGGTCAGCT AGCGCCCAGT GGTGTCCAGC CTCTGCACAA TCGTCTTTTG 
GAACGCTTTT CCCAGCTTAA GCAAAGTTTA TCCGGCATGG GCAGACTGAA ACGTCAGCAC 
TCCGAGAGCA TTGTAAATAC TCCCTTGCCA CCGTTGCCTA CAGAGCAGCG AGTAGCCCCG 
GCGACAGCTT CCTCAAGCTT GAATGCGAAT GCCCACTCCA ACTATGTATA TGATCTAGAT 
GAGATCTACA CGAGACCGGG AGACACTGTA CGACCCGTGG ACCCACTCAG CTCCTATCAA 
ACACTGAGCA AGGAGAGTCT TAGTATTCCT CTGGAGGATA CTGCTGCTCC TCCAGTACCA 
AATCGTCCTC GCTCGCAGAA CTTTATTGGA AACGGATCCA TGGACAGCCC GGAGGTTCCG 
CCGAAACGAC AGCCTACATT GAATTCTCCC AATGCTCCTC CGCTGCCTCC GCGGGGTATT 
ACACCGGACA AGAGGGCGTC AAATCCGTTG ATTTTCAATG ACTTCGGCTC CGGAGATGCC 
GTGGGACGTC GGCATTCTCA GCAGCAGCAG CAGCATGGCC AGAAGTATTC CGTTGTTGAT 
ATTAGCTACG ATGATCCAGA GGCAGATCAG CAGCCGCATT CGCTGCCGCC AAACTTCCAG 
GACGGAAGCG GTGGACACCT CAGCGTCTGT TTTGCCCCGA ATGATTTCCG GGACTCCGGC 
ATATCGACGA CTAGTTCTAG GGAACTGAAC CACATGAATC TTAACAACCT GAGCGAAGAC 
TCATCGTCGA TCTCGACCAT TACAGTGCAG CATCGAGAAC ACTGTCGCAT CAGCAGCAAT 
GGCAGTTTGG ACTATAATGC CGCAGCATCA ATGAACATTA CACAACGAGA GAGTTCGACG 
AGTTCGTTTG ATGTGGAGGA CTTTCCAGTG CCGCCGCCGC CCATTCCACC CAAATCCTTG 
GCAGTGTCAA GCAACGGTGT CAACTTTTCA CTCGACGAGG CCCACTCACC GCATCCCAGC 
ACACAAGCGA ACACCACACA ACTCAATCAC ATTCAGAACC CAAATCAGAA TCATTCCACA 
AACAGTCACC ATCATCTTCA GCAGCAGCAG AATGGAGATG GCGATGGTTA TAGCACGCTG 
CAGCACCCGA TGAACGGGCT GGGGCTGCCT ACCCTTCCAC CACCACCTTC TGCTGTTGAA 
TCGCCAATGC CGGCATCTGC AATAGCCTCA TCCACATCTA GTCATCAGCA CGATGGCGGC 
ACTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg ( NM_001276120.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263049.1 Drosophila melanogaster sponge (spg), transcript variant H, mRNA.

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CloneID	OFa08930
Clone ID Related Accession (Same CDS sequence)	NM_001144646.2 , NM_001276120.1
Accession Version	NM_001276120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sponge, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATT TTGCTGGAGA AAGTCCAAGT GGAGAACCCC 
AACTGGAAGG AGGCTGGCAT CGCTTTCATA GCCTCAGTCA CCAGACTTTT GGAGCGCCTT 
CTGGACTATC GTAGCGTAAT GCAGGGCGAG GAAAATCGGG ATAAGCGCAT GACCTGCACA 
GTCAATCTCC TTAATTTCTA TAAAAACGAA ATCAATCGAA AGGAGATGTA TCTGAGATAC 
ATTTACAAGC TGCACAACCT GCACCTGCAG GCAGAGAATT ACACAGAGGC TGGCTTCACT 
CTGAAACTGT ATGCATCCAT GCTGAGTTGG GATCGTGAGA CCCAGAGCTT TGCCCCCTTC 
GATAATAGTG GTCAGCCAGA GTGGCAGCGC AAGGAGCGTC TATACCACGA GATCCTAAAA 
TACTTCGACA AGGGAAAATG TTGGGAAAAG GGCATTCCAC TGTGCAAGGA GCTGGCTCAT 
TTATACGAAA CGCGGCGTTT TGACTATAAC AAGTTGAGTG AAATACTCAT TCAGGAGGCC 
AAGTTCTTCC AGAATATCCT AACTCAGTTG CGACCGGAGC CGGAATACTT CCGTGTGGGA 
TTCTACGGAA TGGGTCTGCC ACTTTTTGTG CGGAACAAGC AATTTGTGTA CCGTGGACTG 
GAGTACGAGA GAATAGGAGC CTTCACACAG CGTCTGCAAA CGGAGTTTCC TAGTGCTCAG 
ATCTTGGGCA ACAACAGTCC GCCGGACAAC GCCATTCTGA ATGCTCCGGA TCAGTACATA 
CAGATCAGCA ATGTAAGACC TGTTGGGGAT GCACAGGCCT TGAAGACCGC AATGGTTCCT 
GTGCCGGAGA AAATAGCTCG TTTCTACGAG GTCAACGATG TAACCCGATT CATCTACGAT 
CGCCCCATGT ACAAGGGAAC AGTTGACAAG GACAATGAGT TCAAGTCGTT GTGGATAGAA 
CGCACTATTC TAGAGATAGC TAGTCCACTG CCAGGTATTC TACGCTGGTA TGAGGTCAAA 
CAGAAGACGA TGCAGGAGTT AACGCCGGTA GAGTACGCTT GCGAGATTAT CAGCAACGCT 
GGCAAGGAAT TATCCGAGCT GATTGTGCAA TATAAGCGCG ATCCCAAGAG GAATATTAAT 
CCCTTCTCCA TGCGTCTGCA GGGCACAATC GATGCTAATG TCATGGGGGG TATAAGCAAG 
TACCAGGAGG CTTTCTTCTC CGAGCAGTTC CTCAAGTCGC CGCAAGGTGC TGGACAGCAG 
GCAAATGTGC AGCGGCTGAA GGTTCTGATA CTGGAGCAGA TTCAGATATT GGAGCAGGCC 
CTCGAACTGC ACGGTCAGCT AGCGCCCAGT GGTGTCCAGC CTCTGCACAA TCGTCTTTTG 
GAACGCTTTT CCCAGCTTAA GCAAAGTTTA TCCGGCATGG GCAGACTGAA ACGTCAGCAC 
TCCGAGAGCA TTGTAAATAC TCCCTTGCCA CCGTTGCCTA CAGAGCAGCG AGTAGCCCCG 
GCGACAGCTT CCTCAAGCTT GAATGCGAAT GCCCACTCCA ACTATGTATA TGATCTAGAT 
GAGATCTACA CGAGACCGGG AGACACTGTA CGACCCGTGG ACCCACTCAG CTCCTATCAA 
ACACTGAGCA AGGAGAGTCT TAGTATTCCT CTGGAGGATA CTGCTGCTCC TCCAGTACCA 
AATCGTCCTC GCTCGCAGAA CTTTATTGGA AACGGATCCA TGGACAGCCC GGAGGTTCCG 
CCGAAACGAC AGCCTACATT GAATTCTCCC AATGCTCCTC CGCTGCCTCC GCGGGGTATT 
ACACCGGACA AGAGGGCGTC AAATCCGTTG ATTTTCAATG ACTTCGGCTC CGGAGATGCC 
GTGGGACGTC GGCATTCTCA GCAGCAGCAG CAGCATGGCC AGAAGTATTC CGTTGTTGAT 
ATTAGCTACG ATGATCCAGA GGCAGATCAG CAGCCGCATT CGCTGCCGCC AAACTTCCAG 
GACGGAAGCG GTGGACACCT CAGCGTCTGT TTTGCCCCGA ATGATTTCCG GGACTCCGGC 
ATATCGACGA CTAGTTCTAG GGAACTGAAC CACATGAATC TTAACAACCT GAGCGAAGAC 
TCATCGTCGA TCTCGACCAT TACAGTGCAG CATCGAGAAC ACTGTCGCAT CAGCAGCAAT 
GGCAGTTTGG ACTATAATGC CGCAGCATCA ATGAACATTA CACAACGAGA GAGTTCGACG 
AGTTCGTTTG ATGTGGAGGA CTTTCCAGTG CCGCCGCCGC CCATTCCACC CAAATCCTTG 
GCAGTGTCAA GCAACGGTGT CAACTTTTCA CTCGACGAGG CCCACTCACC GCATCCCAGC 
ACACAAGCGA ACACCACACA ACTCAATCAC ATTCAGAACC CAAATCAGAA TCATTCCACA 
AACAGTCACC ATCATCTTCA GCAGCAGCAG AATGGAGATG GCGATGGTTA TAGCACGCTG 
CAGCACCCGA TGAACGGGCT GGGGCTGCCT ACCCTTCCAC CACCACCTTC TGCTGTTGAA 
TCGCCAATGC CGGCATCTGC AATAGCCTCA TCCACATCTA GTCATCAGCA CGATGGCGGC 
ACTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263049.1
CDS	668..6676
Translation	MWIPSNNKYGVAIHNWHGDVRFGLALDVGDSVDIVEECKHWYRGSCPRKSRAVGLFPKTYIHIKDLSKIDPVVAECTQVLREWSEIWKRLYVDREAYKFQTLRKVMFSILESRRELLSATMTQDQTLELQMVVVSKIDWGNRKLGLDLVPREGPLAVDPHGIGIVKLYNVHVSSADNAKASSSRGTLRRKTQRKILTHHLYFCMRDFGHRIGGDDAEVYFFLYDGSHMRPLSERFLVKISKDGFSNYIERLHSNCTVFTDLGAADLNEELHLVAVVMRVGKIIQSDSIKKIEKSGTLGHGPTFRRPFGVGVLSLGDIAHFDSSLEQSSDEREYNFKLFQCEEKDFHLLPELIIKKSSGKYSPIQASQGTQGIVVSLKLLHGGLGQARQEQPMLFQGSTITRKMGFPDVIMPGDVRNDLFLNLERGEFERGGKNTGKNILVTVVVLDVAGNVLTDCLWGASGMESQPQYRSMILYHQNAPSWNEMLRLSVPIDKFSTAHVRFEFRHCSTRDKTEPKLFAFSFARLMDTGGATLGDGLHELYVYKCEDPAKLQVANYLRLQCRPRDAQAKMDCGGCFSRSSKEVFVLRSLLCSTKLTQNADLLSLLQWRTYPETIQDSLTGVLRLNDEELVKFLQDVLDALFAMFSNEEGNSTQHSGLVFHVLVSIFSLLQSNKFQHFRPVMNEYIENHFAAALVYKGLITSVEHMAVFMTKAEHPDPFQKCFGSLEYIFKLLIQSRKLFARATGGQYEDSFKRDLHSLFTALNGMLAVPSYDVIIPTQEALLNSTGVVLEQLKDTLPAPELGMLARNMLDAIPRGAPIRLIQAKLHAVKDLVSGELFHEDDSRTVVLSVACKHLRMHLSRRDELRLCAEILSEILSQLYDLQKEQREKVTNTLQHDLDSLCKNILGILIRTISIIMEGSNAVLPQLVSCLLGLLQLLDETHYKRYWDELSPNKDPRDLKEFLSKSLLVYEELLTQDWHVFPNDWLVMKLAANDVLRMSLEEFAKPLVYRFLGPQAFDSQLWWSYFSLAVAFLTQPSLQLEQYREPKRLKILHSHGDMRVLMGFQILSMWSQLGEQKLHFIPSMVGPFLEVTLVPEPALRKATLSVFYDMMQCEQAARGSFRLVESELIDKLDLLISENKGDDEYRELFSTILLEKVQVENPNWKEAGIAFIASVTRLLERLLDYRSVMQGEENRDKRMTCTVNLLNFYKNEINRKEMYLRYIYKLHNLHLQAENYTEAGFTLKLYASMLSWDRETQSFAPFDNSGQPEWQRKERLYHEILKYFDKGKCWEKGIPLCKELAHLYETRRFDYNKLSEILIQEAKFFQNILTQLRPEPEYFRVGFYGMGLPLFVRNKQFVYRGLEYERIGAFTQRLQTEFPSAQILGNNSPPDNAILNAPDQYIQISNVRPVGDAQALKTAMVPVPEKIARFYEVNDVTRFIYDRPMYKGTVDKDNEFKSLWIERTILEIASPLPGILRWYEVKQKTMQELTPVEYACEIISNAGKELSELIVQYKRDPKRNINPFSMRLQGTIDANVMGGISKYQEAFFSEQFLKSPQGAGQQANVQRLKVLILEQIQILEQALELHGQLAPSGVQPLHNRLLERFSQLKQSLSGMGRLKRQHSESIVNTPLPPLPTEQRVAPATASSSLNANAHSNYVYDLDEIYTRPGDTVRPVDPLSSYQTLSKESLSIPLEDTAAPPVPNRPRSQNFIGNGSMDSPEVPPKRQPTLNSPNAPPLPPRGITPDKRASNPLIFNDFGSGDAVGRRHSQQQQQHGQKYSVVDISYDDPEADQQPHSLPPNFQDGSGGHLSVCFAPNDFRDSGISTTSSRELNHMNLNNLSEDSSSISTITVQHREHCRISSNGSLDYNAAASMNITQRESSTSSFDVEDFPVPPPPIPPKSLAVSSNGVNFSLDEAHSPHPSTQANTTQLNHIQNPNQNHSTNSHHHLQQQQNGDGDGYSTLQHPMNGLGLPTLPPPPSAVESPMPASAIASSTSSHQHDGGTF

Target ORF information:

RefSeq Version	NM_001276120.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sponge (spg), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276120.1

ORF Insert Sequence:

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATT TTGCTGGAGA AAGTCCAAGT GGAGAACCCC 
AACTGGAAGG AGGCTGGCAT CGCTTTCATA GCCTCAGTCA CCAGACTTTT GGAGCGCCTT 
CTGGACTATC GTAGCGTAAT GCAGGGCGAG GAAAATCGGG ATAAGCGCAT GACCTGCACA 
GTCAATCTCC TTAATTTCTA TAAAAACGAA ATCAATCGAA AGGAGATGTA TCTGAGATAC 
ATTTACAAGC TGCACAACCT GCACCTGCAG GCAGAGAATT ACACAGAGGC TGGCTTCACT 
CTGAAACTGT ATGCATCCAT GCTGAGTTGG GATCGTGAGA CCCAGAGCTT TGCCCCCTTC 
GATAATAGTG GTCAGCCAGA GTGGCAGCGC AAGGAGCGTC TATACCACGA GATCCTAAAA 
TACTTCGACA AGGGAAAATG TTGGGAAAAG GGCATTCCAC TGTGCAAGGA GCTGGCTCAT 
TTATACGAAA CGCGGCGTTT TGACTATAAC AAGTTGAGTG AAATACTCAT TCAGGAGGCC 
AAGTTCTTCC AGAATATCCT AACTCAGTTG CGACCGGAGC CGGAATACTT CCGTGTGGGA 
TTCTACGGAA TGGGTCTGCC ACTTTTTGTG CGGAACAAGC AATTTGTGTA CCGTGGACTG 
GAGTACGAGA GAATAGGAGC CTTCACACAG CGTCTGCAAA CGGAGTTTCC TAGTGCTCAG 
ATCTTGGGCA ACAACAGTCC GCCGGACAAC GCCATTCTGA ATGCTCCGGA TCAGTACATA 
CAGATCAGCA ATGTAAGACC TGTTGGGGAT GCACAGGCCT TGAAGACCGC AATGGTTCCT 
GTGCCGGAGA AAATAGCTCG TTTCTACGAG GTCAACGATG TAACCCGATT CATCTACGAT 
CGCCCCATGT ACAAGGGAAC AGTTGACAAG GACAATGAGT TCAAGTCGTT GTGGATAGAA 
CGCACTATTC TAGAGATAGC TAGTCCACTG CCAGGTATTC TACGCTGGTA TGAGGTCAAA 
CAGAAGACGA TGCAGGAGTT AACGCCGGTA GAGTACGCTT GCGAGATTAT CAGCAACGCT 
GGCAAGGAAT TATCCGAGCT GATTGTGCAA TATAAGCGCG ATCCCAAGAG GAATATTAAT 
CCCTTCTCCA TGCGTCTGCA GGGCACAATC GATGCTAATG TCATGGGGGG TATAAGCAAG 
TACCAGGAGG CTTTCTTCTC CGAGCAGTTC CTCAAGTCGC CGCAAGGTGC TGGACAGCAG 
GCAAATGTGC AGCGGCTGAA GGTTCTGATA CTGGAGCAGA TTCAGATATT GGAGCAGGCC 
CTCGAACTGC ACGGTCAGCT AGCGCCCAGT GGTGTCCAGC CTCTGCACAA TCGTCTTTTG 
GAACGCTTTT CCCAGCTTAA GCAAAGTTTA TCCGGCATGG GCAGACTGAA ACGTCAGCAC 
TCCGAGAGCA TTGTAAATAC TCCCTTGCCA CCGTTGCCTA CAGAGCAGCG AGTAGCCCCG 
GCGACAGCTT CCTCAAGCTT GAATGCGAAT GCCCACTCCA ACTATGTATA TGATCTAGAT 
GAGATCTACA CGAGACCGGG AGACACTGTA CGACCCGTGG ACCCACTCAG CTCCTATCAA 
ACACTGAGCA AGGAGAGTCT TAGTATTCCT CTGGAGGATA CTGCTGCTCC TCCAGTACCA 
AATCGTCCTC GCTCGCAGAA CTTTATTGGA AACGGATCCA TGGACAGCCC GGAGGTTCCG 
CCGAAACGAC AGCCTACATT GAATTCTCCC AATGCTCCTC CGCTGCCTCC GCGGGGTATT 
ACACCGGACA AGAGGGCGTC AAATCCGTTG ATTTTCAATG ACTTCGGCTC CGGAGATGCC 
GTGGGACGTC GGCATTCTCA GCAGCAGCAG CAGCATGGCC AGAAGTATTC CGTTGTTGAT 
ATTAGCTACG ATGATCCAGA GGCAGATCAG CAGCCGCATT CGCTGCCGCC AAACTTCCAG 
GACGGAAGCG GTGGACACCT CAGCGTCTGT TTTGCCCCGA ATGATTTCCG GGACTCCGGC 
ATATCGACGA CTAGTTCTAG GGAACTGAAC CACATGAATC TTAACAACCT GAGCGAAGAC 
TCATCGTCGA TCTCGACCAT TACAGTGCAG CATCGAGAAC ACTGTCGCAT CAGCAGCAAT 
GGCAGTTTGG ACTATAATGC CGCAGCATCA ATGAACATTA CACAACGAGA GAGTTCGACG 
AGTTCGTTTG ATGTGGAGGA CTTTCCAGTG CCGCCGCCGC CCATTCCACC CAAATCCTTG 
GCAGTGTCAA GCAACGGTGT CAACTTTTCA CTCGACGAGG CCCACTCACC GCATCCCAGC 
ACACAAGCGA ACACCACACA ACTCAATCAC ATTCAGAACC CAAATCAGAA TCATTCCACA 
AACAGTCACC ATCATCTTCA GCAGCAGCAG AATGGAGATG GCGATGGTTA TAGCACGCTG 
CAGCACCCGA TGAACGGGCT GGGGCTGCCT ACCCTTCCAC CACCACCTTC TGCTGTTGAA 
TCGCCAATGC CGGCATCTGC AATAGCCTCA TCCACATCTA GTCATCAGCA CGATGGCGGC 
ACTTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg ( NM_001276118.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263047.1 Drosophila melanogaster sponge (spg), transcript variant F, mRNA.

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CloneID	OFa08931
Clone ID Related Accession (Same CDS sequence)	NM_001276118.1 , NM_001276119.1
Accession Version	NM_001276118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sponge, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATG GAACATCTCA GCTTGGTTTT GCTGGAGAAA 
GTCCAAGTGG AGAACCCCAA CTGGAAGGAG GCTGGCATCG CTTTCATAGC CTCAGTCACC 
AGACTTTTGG AGCGCCTTCT GGACTATCGT AGCGTAATGC AGGGCGAGGA AAATCGGGAT 
AAGCGCATGA CCTGCACAGT CAATCTCCTT AATTTCTATA AAAACGAAAT CAATCGAAAG 
GAGATGTATC TGAGATACAT TTACAAGCTG CACAACCTGC ACCTGCAGGC AGAGAATTAC 
ACAGAGGCTG GCTTCACTCT GAAACTGTAT GCATCCATGC TGAGTTGGGA TCGTGAGACC 
CAGAGCTTTG CCCCCTTCGA TAATAGTGGT CAGCCAGAGT GGCAGCGCAA GGAGCGTCTA 
TACCACGAGA TCCTAAAATA CTTCGACAAG GGAAAATGTT GGGAAAAGGG CATTCCACTG 
TGCAAGGAGC TGGCTCATTT ATACGAAACG CGGCGTTTTG ACTATAACAA GTTGAGTGAA 
ATACTCATTC AGGAGGCCAA GTTCTTCCAG AATATCCTAA CTCAGTTGCG ACCGGAGCCG 
GAATACTTCC GTGTGGGATT CTACGGAATG GGTCTGCCAC TTTTTGTGCG GAACAAGCAA 
TTTGTGTACC GTGGACTGGA GTACGAGAGA ATAGGAGCCT TCACACAGCG TCTGCAAACG 
GAGTTTCCTA GTGCTCAGAT CTTGGGCAAC AACAGTCCGC CGGACAACGC CATTCTGAAT 
GCTCCGGATC AGTACATACA GATCAGCAAT GTAAGACCTG TTGGGGATGC ACAGGCCTTG 
AAGACCGCAA TGGTTCCTGT GCCGGAGAAA ATAGCTCGTT TCTACGAGGT CAACGATGTA 
ACCCGATTCA TCTACGATCG CCCCATGTAC AAGGGAACAG TTGACAAGGA CAATGAGTTC 
AAGTCGTTGT GGATAGAACG CACTATTCTA GAGATAGCTA GTCCACTGCC AGGTATTCTA 
CGCTGGTATG AGGTCAAACA GAAGACGATG CAGGAGTTAA CGCCGGTAGA GTACGCTTGC 
GAGATTATCA GCAACGCTGG CAAGGAATTA TCCGAGCTGA TTGTGCAATA TAAGCGCGAT 
CCCAAGAGGA ATATTAATCC CTTCTCCATG CGTCTGCAGG GCACAATCGA TGCTAATGTC 
ATGGGGGGTA TAAGCAAGTA CCAGGAGGCT TTCTTCTCCG AGCAGTTCCT CAAGTCGCCG 
CAAGGTGCTG GACAGCAGGC AAATGTGCAG CGGCTGAAGG TTCTGATACT GGAGCAGATT 
CAGATATTGG AGCAGGCCCT CGAACTGCAC GGTCAGCTAG CGCCCAGTGG TGTCCAGCCT 
CTGCACAATC GTCTTTTGGA ACGCTTTTCC CAGCTTAAGC AAAGTTTATC CGGCATGGGC 
AGACTGAAAC GTCAGCACTC CGAGAGCATT GTAAATACTC CCTTGCCACC GTTGCCTACA 
GAGCAGCGAG TAGCCCCGGC GACAGCTTCC TCAAGCTTGA ATGCGAATGC CCACTCCAAC 
TATGTATATG ATCTAGATGA GATCTACACG AGACCGGGAG ACACTGTACG ACCCGTGGAC 
CCACTCAGCT CCTATCAAAC ACTGAGCAAG GAGAGTCTTA GTATTCCTCT GGAGGATACT 
GCTGCTCCTC CAGTACCAAA TCGTCCTCGC TCGCAGAACT TTATTGGAAA CGGATCCATG 
GACAGCCCGG AGGTTCCGCC GAAACGACAG CCTACATTGA ATTCTCCCAA TGCTCCTCCG 
CTGCCTCCGC GGGGTATTAC ACCGGACAAG AGGGCGTCAA ATCCGTTGAT TTTCAATGAC 
TTCGGCTCCG GAGATGCCGT GGGACGTCGG CATTCTCAGC AGCAGCAGCA GCATGGCCAG 
AAGTATTCCG TTGTTGATAT TAGCTACGAT GATCCAGAGG CAGATCAGCA GCCGCATTCG 
CTGCCGCCAA ACTTCCAGGA CGGAAGCGGT GGACACCTCA GCGTCTGTTT TGCCCCGAAT 
GATTTCCGGG ACTCCGGCAT ATCGACGACT AGTTCTAGGG AACTGAACCA CATGAATCTT 
AACAACCTGA GCGAAGACTC ATCGTCGATC TCGACCATTA CAGTGCAGCA TCGAGAACAC 
TGTCGCATCA GCAGCAATGG CAGTTTGGAC TATAATGCCG CAGCATCAAT GAACATTACA 
CAACGAGAGA GTTCGACGAG TTCGTTTGAT GTGGAGGACT TTCCAGTGCC GCCGCCGCCC 
ATTCCACCCA AATCCTTGGC AGTGTCAAGC AACGGTGTCA ACTTTTCACT CGACGAGGCC 
CACTCACCGC ATCCCAGCAC ACAAGCGAAC ACCACACAAC TCAATCACAT TCAGAACCCA 
AATCAGAATC ATTCCACAAA CAGTCACCAT CATCTTCAGC AGCAGCAGAA TGGAGATGGC 
GATGGTTATA GCACGCTGCA GCACCCGATG AACGGGCTGG GGCTGCCTAC CCTTCCACCA 
CCACCTTCTG CTGTTGAATC GCCAATGCCG GCATCTGCAA TAGCCTCATC CACATCTAGT 
CATCAGCACG ATGGCGGCAC TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263047.1
CDS	129..6155
Translation	MWIPSNNKYGVAIHNWHGDVRFGLALDVGDSVDIVEECKHWYRGSCPRKSRAVGLFPKTYIHIKDLSKIDPVVAECTQVLREWSEIWKRLYVDREAYKFQTLRKVMFSILESRRELLSATMTQDQTLELQMVVVSKIDWGNRKLGLDLVPREGPLAVDPHGIGIVKLYNVHVSSADNAKASSSRGTLRRKTQRKILTHHLYFCMRDFGHRIGGDDAEVYFFLYDGSHMRPLSERFLVKISKDGFSNYIERLHSNCTVFTDLGAADLNEELHLVAVVMRVGKIIQSDSIKKIEKSGTLGHGPTFRRPFGVGVLSLGDIAHFDSSLEQSSDEREYNFKLFQCEEKDFHLLPELIIKKSSGKYSPIQASQGTQGIVVSLKLLHGGLGQARQEQPMLFQGSTITRKMGFPDVIMPGDVRNDLFLNLERGEFERGGKNTGKNILVTVVVLDVAGNVLTDCLWGASGMESQPQYRSMILYHQNAPSWNEMLRLSVPIDKFSTAHVRFEFRHCSTRDKTEPKLFAFSFARLMDTGGATLGDGLHELYVYKCEDPAKLQVANYLRLQCRPRDAQAKMDCGGCFSRSSKEVFVLRSLLCSTKLTQNADLLSLLQWRTYPETIQDSLTGVLRLNDEELVKFLQDVLDALFAMFSNEEGNSTQHSGLVFHVLVSIFSLLQSNKFQHFRPVMNEYIENHFAAALVYKGLITSVEHMAVFMTKAEHPDPFQKCFGSLEYIFKLLIQSRKLFARATGGQYEDSFKRDLHSLFTALNGMLAVPSYDVIIPTQEALLNSTGVVLEQLKDTLPAPELGMLARNMLDAIPRGAPIRLIQAKLHAVKDLVSGELFHEDDSRTVVLSVACKHLRMHLSRRDELRLCAEILSEILSQLYDLQKEQREKVTNTLQHDLDSLCKNILGILIRTISIIMEGSNAVLPQLVSCLLGLLQLLDETHYKRYWDELSPNKDPRDLKEFLSKSLLVYEELLTQDWHVFPNDWLVMKLAANDVLRMSLEEFAKPLVYRFLGPQAFDSQLWWSYFSLAVAFLTQPSLQLEQYREPKRLKILHSHGDMRVLMGFQILSMWSQLGEQKLHFIPSMVGPFLEVTLVPEPALRKATLSVFYDMMQCEQAARGSFRLVESELIDKLDLLISENKGDDEYRELFSTMEHLSLVLLEKVQVENPNWKEAGIAFIASVTRLLERLLDYRSVMQGEENRDKRMTCTVNLLNFYKNEINRKEMYLRYIYKLHNLHLQAENYTEAGFTLKLYASMLSWDRETQSFAPFDNSGQPEWQRKERLYHEILKYFDKGKCWEKGIPLCKELAHLYETRRFDYNKLSEILIQEAKFFQNILTQLRPEPEYFRVGFYGMGLPLFVRNKQFVYRGLEYERIGAFTQRLQTEFPSAQILGNNSPPDNAILNAPDQYIQISNVRPVGDAQALKTAMVPVPEKIARFYEVNDVTRFIYDRPMYKGTVDKDNEFKSLWIERTILEIASPLPGILRWYEVKQKTMQELTPVEYACEIISNAGKELSELIVQYKRDPKRNINPFSMRLQGTIDANVMGGISKYQEAFFSEQFLKSPQGAGQQANVQRLKVLILEQIQILEQALELHGQLAPSGVQPLHNRLLERFSQLKQSLSGMGRLKRQHSESIVNTPLPPLPTEQRVAPATASSSLNANAHSNYVYDLDEIYTRPGDTVRPVDPLSSYQTLSKESLSIPLEDTAAPPVPNRPRSQNFIGNGSMDSPEVPPKRQPTLNSPNAPPLPPRGITPDKRASNPLIFNDFGSGDAVGRRHSQQQQQHGQKYSVVDISYDDPEADQQPHSLPPNFQDGSGGHLSVCFAPNDFRDSGISTTSSRELNHMNLNNLSEDSSSISTITVQHREHCRISSNGSLDYNAAASMNITQRESSTSSFDVEDFPVPPPPIPPKSLAVSSNGVNFSLDEAHSPHPSTQANTTQLNHIQNPNQNHSTNSHHHLQQQQNGDGDGYSTLQHPMNGLGLPTLPPPPSAVESPMPASAIASSTSSHQHDGGTF

Target ORF information:

RefSeq Version	NM_001276118.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sponge (spg), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276118.1

ORF Insert Sequence:

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATG GAACATCTCA GCTTGGTTTT GCTGGAGAAA 
GTCCAAGTGG AGAACCCCAA CTGGAAGGAG GCTGGCATCG CTTTCATAGC CTCAGTCACC 
AGACTTTTGG AGCGCCTTCT GGACTATCGT AGCGTAATGC AGGGCGAGGA AAATCGGGAT 
AAGCGCATGA CCTGCACAGT CAATCTCCTT AATTTCTATA AAAACGAAAT CAATCGAAAG 
GAGATGTATC TGAGATACAT TTACAAGCTG CACAACCTGC ACCTGCAGGC AGAGAATTAC 
ACAGAGGCTG GCTTCACTCT GAAACTGTAT GCATCCATGC TGAGTTGGGA TCGTGAGACC 
CAGAGCTTTG CCCCCTTCGA TAATAGTGGT CAGCCAGAGT GGCAGCGCAA GGAGCGTCTA 
TACCACGAGA TCCTAAAATA CTTCGACAAG GGAAAATGTT GGGAAAAGGG CATTCCACTG 
TGCAAGGAGC TGGCTCATTT ATACGAAACG CGGCGTTTTG ACTATAACAA GTTGAGTGAA 
ATACTCATTC AGGAGGCCAA GTTCTTCCAG AATATCCTAA CTCAGTTGCG ACCGGAGCCG 
GAATACTTCC GTGTGGGATT CTACGGAATG GGTCTGCCAC TTTTTGTGCG GAACAAGCAA 
TTTGTGTACC GTGGACTGGA GTACGAGAGA ATAGGAGCCT TCACACAGCG TCTGCAAACG 
GAGTTTCCTA GTGCTCAGAT CTTGGGCAAC AACAGTCCGC CGGACAACGC CATTCTGAAT 
GCTCCGGATC AGTACATACA GATCAGCAAT GTAAGACCTG TTGGGGATGC ACAGGCCTTG 
AAGACCGCAA TGGTTCCTGT GCCGGAGAAA ATAGCTCGTT TCTACGAGGT CAACGATGTA 
ACCCGATTCA TCTACGATCG CCCCATGTAC AAGGGAACAG TTGACAAGGA CAATGAGTTC 
AAGTCGTTGT GGATAGAACG CACTATTCTA GAGATAGCTA GTCCACTGCC AGGTATTCTA 
CGCTGGTATG AGGTCAAACA GAAGACGATG CAGGAGTTAA CGCCGGTAGA GTACGCTTGC 
GAGATTATCA GCAACGCTGG CAAGGAATTA TCCGAGCTGA TTGTGCAATA TAAGCGCGAT 
CCCAAGAGGA ATATTAATCC CTTCTCCATG CGTCTGCAGG GCACAATCGA TGCTAATGTC 
ATGGGGGGTA TAAGCAAGTA CCAGGAGGCT TTCTTCTCCG AGCAGTTCCT CAAGTCGCCG 
CAAGGTGCTG GACAGCAGGC AAATGTGCAG CGGCTGAAGG TTCTGATACT GGAGCAGATT 
CAGATATTGG AGCAGGCCCT CGAACTGCAC GGTCAGCTAG CGCCCAGTGG TGTCCAGCCT 
CTGCACAATC GTCTTTTGGA ACGCTTTTCC CAGCTTAAGC AAAGTTTATC CGGCATGGGC 
AGACTGAAAC GTCAGCACTC CGAGAGCATT GTAAATACTC CCTTGCCACC GTTGCCTACA 
GAGCAGCGAG TAGCCCCGGC GACAGCTTCC TCAAGCTTGA ATGCGAATGC CCACTCCAAC 
TATGTATATG ATCTAGATGA GATCTACACG AGACCGGGAG ACACTGTACG ACCCGTGGAC 
CCACTCAGCT CCTATCAAAC ACTGAGCAAG GAGAGTCTTA GTATTCCTCT GGAGGATACT 
GCTGCTCCTC CAGTACCAAA TCGTCCTCGC TCGCAGAACT TTATTGGAAA CGGATCCATG 
GACAGCCCGG AGGTTCCGCC GAAACGACAG CCTACATTGA ATTCTCCCAA TGCTCCTCCG 
CTGCCTCCGC GGGGTATTAC ACCGGACAAG AGGGCGTCAA ATCCGTTGAT TTTCAATGAC 
TTCGGCTCCG GAGATGCCGT GGGACGTCGG CATTCTCAGC AGCAGCAGCA GCATGGCCAG 
AAGTATTCCG TTGTTGATAT TAGCTACGAT GATCCAGAGG CAGATCAGCA GCCGCATTCG 
CTGCCGCCAA ACTTCCAGGA CGGAAGCGGT GGACACCTCA GCGTCTGTTT TGCCCCGAAT 
GATTTCCGGG ACTCCGGCAT ATCGACGACT AGTTCTAGGG AACTGAACCA CATGAATCTT 
AACAACCTGA GCGAAGACTC ATCGTCGATC TCGACCATTA CAGTGCAGCA TCGAGAACAC 
TGTCGCATCA GCAGCAATGG CAGTTTGGAC TATAATGCCG CAGCATCAAT GAACATTACA 
CAACGAGAGA GTTCGACGAG TTCGTTTGAT GTGGAGGACT TTCCAGTGCC GCCGCCGCCC 
ATTCCACCCA AATCCTTGGC AGTGTCAAGC AACGGTGTCA ACTTTTCACT CGACGAGGCC 
CACTCACCGC ATCCCAGCAC ACAAGCGAAC ACCACACAAC TCAATCACAT TCAGAACCCA 
AATCAGAATC ATTCCACAAA CAGTCACCAT CATCTTCAGC AGCAGCAGAA TGGAGATGGC 
GATGGTTATA GCACGCTGCA GCACCCGATG AACGGGCTGG GGCTGCCTAC CCTTCCACCA 
CCACCTTCTG CTGTTGAATC GCCAATGCCG GCATCTGCAA TAGCCTCATC CACATCTAGT 
CATCAGCACG ATGGCGGCAC TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spg ( NM_001276119.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263048.1 Drosophila melanogaster sponge (spg), transcript variant G, mRNA.

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CloneID	OFa08931
Clone ID Related Accession (Same CDS sequence)	NM_001276118.1 , NM_001276119.1
Accession Version	NM_001276119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	sponge, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATG GAACATCTCA GCTTGGTTTT GCTGGAGAAA 
GTCCAAGTGG AGAACCCCAA CTGGAAGGAG GCTGGCATCG CTTTCATAGC CTCAGTCACC 
AGACTTTTGG AGCGCCTTCT GGACTATCGT AGCGTAATGC AGGGCGAGGA AAATCGGGAT 
AAGCGCATGA CCTGCACAGT CAATCTCCTT AATTTCTATA AAAACGAAAT CAATCGAAAG 
GAGATGTATC TGAGATACAT TTACAAGCTG CACAACCTGC ACCTGCAGGC AGAGAATTAC 
ACAGAGGCTG GCTTCACTCT GAAACTGTAT GCATCCATGC TGAGTTGGGA TCGTGAGACC 
CAGAGCTTTG CCCCCTTCGA TAATAGTGGT CAGCCAGAGT GGCAGCGCAA GGAGCGTCTA 
TACCACGAGA TCCTAAAATA CTTCGACAAG GGAAAATGTT GGGAAAAGGG CATTCCACTG 
TGCAAGGAGC TGGCTCATTT ATACGAAACG CGGCGTTTTG ACTATAACAA GTTGAGTGAA 
ATACTCATTC AGGAGGCCAA GTTCTTCCAG AATATCCTAA CTCAGTTGCG ACCGGAGCCG 
GAATACTTCC GTGTGGGATT CTACGGAATG GGTCTGCCAC TTTTTGTGCG GAACAAGCAA 
TTTGTGTACC GTGGACTGGA GTACGAGAGA ATAGGAGCCT TCACACAGCG TCTGCAAACG 
GAGTTTCCTA GTGCTCAGAT CTTGGGCAAC AACAGTCCGC CGGACAACGC CATTCTGAAT 
GCTCCGGATC AGTACATACA GATCAGCAAT GTAAGACCTG TTGGGGATGC ACAGGCCTTG 
AAGACCGCAA TGGTTCCTGT GCCGGAGAAA ATAGCTCGTT TCTACGAGGT CAACGATGTA 
ACCCGATTCA TCTACGATCG CCCCATGTAC AAGGGAACAG TTGACAAGGA CAATGAGTTC 
AAGTCGTTGT GGATAGAACG CACTATTCTA GAGATAGCTA GTCCACTGCC AGGTATTCTA 
CGCTGGTATG AGGTCAAACA GAAGACGATG CAGGAGTTAA CGCCGGTAGA GTACGCTTGC 
GAGATTATCA GCAACGCTGG CAAGGAATTA TCCGAGCTGA TTGTGCAATA TAAGCGCGAT 
CCCAAGAGGA ATATTAATCC CTTCTCCATG CGTCTGCAGG GCACAATCGA TGCTAATGTC 
ATGGGGGGTA TAAGCAAGTA CCAGGAGGCT TTCTTCTCCG AGCAGTTCCT CAAGTCGCCG 
CAAGGTGCTG GACAGCAGGC AAATGTGCAG CGGCTGAAGG TTCTGATACT GGAGCAGATT 
CAGATATTGG AGCAGGCCCT CGAACTGCAC GGTCAGCTAG CGCCCAGTGG TGTCCAGCCT 
CTGCACAATC GTCTTTTGGA ACGCTTTTCC CAGCTTAAGC AAAGTTTATC CGGCATGGGC 
AGACTGAAAC GTCAGCACTC CGAGAGCATT GTAAATACTC CCTTGCCACC GTTGCCTACA 
GAGCAGCGAG TAGCCCCGGC GACAGCTTCC TCAAGCTTGA ATGCGAATGC CCACTCCAAC 
TATGTATATG ATCTAGATGA GATCTACACG AGACCGGGAG ACACTGTACG ACCCGTGGAC 
CCACTCAGCT CCTATCAAAC ACTGAGCAAG GAGAGTCTTA GTATTCCTCT GGAGGATACT 
GCTGCTCCTC CAGTACCAAA TCGTCCTCGC TCGCAGAACT TTATTGGAAA CGGATCCATG 
GACAGCCCGG AGGTTCCGCC GAAACGACAG CCTACATTGA ATTCTCCCAA TGCTCCTCCG 
CTGCCTCCGC GGGGTATTAC ACCGGACAAG AGGGCGTCAA ATCCGTTGAT TTTCAATGAC 
TTCGGCTCCG GAGATGCCGT GGGACGTCGG CATTCTCAGC AGCAGCAGCA GCATGGCCAG 
AAGTATTCCG TTGTTGATAT TAGCTACGAT GATCCAGAGG CAGATCAGCA GCCGCATTCG 
CTGCCGCCAA ACTTCCAGGA CGGAAGCGGT GGACACCTCA GCGTCTGTTT TGCCCCGAAT 
GATTTCCGGG ACTCCGGCAT ATCGACGACT AGTTCTAGGG AACTGAACCA CATGAATCTT 
AACAACCTGA GCGAAGACTC ATCGTCGATC TCGACCATTA CAGTGCAGCA TCGAGAACAC 
TGTCGCATCA GCAGCAATGG CAGTTTGGAC TATAATGCCG CAGCATCAAT GAACATTACA 
CAACGAGAGA GTTCGACGAG TTCGTTTGAT GTGGAGGACT TTCCAGTGCC GCCGCCGCCC 
ATTCCACCCA AATCCTTGGC AGTGTCAAGC AACGGTGTCA ACTTTTCACT CGACGAGGCC 
CACTCACCGC ATCCCAGCAC ACAAGCGAAC ACCACACAAC TCAATCACAT TCAGAACCCA 
AATCAGAATC ATTCCACAAA CAGTCACCAT CATCTTCAGC AGCAGCAGAA TGGAGATGGC 
GATGGTTATA GCACGCTGCA GCACCCGATG AACGGGCTGG GGCTGCCTAC CCTTCCACCA 
CCACCTTCTG CTGTTGAATC GCCAATGCCG GCATCTGCAA TAGCCTCATC CACATCTAGT 
CATCAGCACG ATGGCGGCAC TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263048.1
CDS	668..6694
Translation	MWIPSNNKYGVAIHNWHGDVRFGLALDVGDSVDIVEECKHWYRGSCPRKSRAVGLFPKTYIHIKDLSKIDPVVAECTQVLREWSEIWKRLYVDREAYKFQTLRKVMFSILESRRELLSATMTQDQTLELQMVVVSKIDWGNRKLGLDLVPREGPLAVDPHGIGIVKLYNVHVSSADNAKASSSRGTLRRKTQRKILTHHLYFCMRDFGHRIGGDDAEVYFFLYDGSHMRPLSERFLVKISKDGFSNYIERLHSNCTVFTDLGAADLNEELHLVAVVMRVGKIIQSDSIKKIEKSGTLGHGPTFRRPFGVGVLSLGDIAHFDSSLEQSSDEREYNFKLFQCEEKDFHLLPELIIKKSSGKYSPIQASQGTQGIVVSLKLLHGGLGQARQEQPMLFQGSTITRKMGFPDVIMPGDVRNDLFLNLERGEFERGGKNTGKNILVTVVVLDVAGNVLTDCLWGASGMESQPQYRSMILYHQNAPSWNEMLRLSVPIDKFSTAHVRFEFRHCSTRDKTEPKLFAFSFARLMDTGGATLGDGLHELYVYKCEDPAKLQVANYLRLQCRPRDAQAKMDCGGCFSRSSKEVFVLRSLLCSTKLTQNADLLSLLQWRTYPETIQDSLTGVLRLNDEELVKFLQDVLDALFAMFSNEEGNSTQHSGLVFHVLVSIFSLLQSNKFQHFRPVMNEYIENHFAAALVYKGLITSVEHMAVFMTKAEHPDPFQKCFGSLEYIFKLLIQSRKLFARATGGQYEDSFKRDLHSLFTALNGMLAVPSYDVIIPTQEALLNSTGVVLEQLKDTLPAPELGMLARNMLDAIPRGAPIRLIQAKLHAVKDLVSGELFHEDDSRTVVLSVACKHLRMHLSRRDELRLCAEILSEILSQLYDLQKEQREKVTNTLQHDLDSLCKNILGILIRTISIIMEGSNAVLPQLVSCLLGLLQLLDETHYKRYWDELSPNKDPRDLKEFLSKSLLVYEELLTQDWHVFPNDWLVMKLAANDVLRMSLEEFAKPLVYRFLGPQAFDSQLWWSYFSLAVAFLTQPSLQLEQYREPKRLKILHSHGDMRVLMGFQILSMWSQLGEQKLHFIPSMVGPFLEVTLVPEPALRKATLSVFYDMMQCEQAARGSFRLVESELIDKLDLLISENKGDDEYRELFSTMEHLSLVLLEKVQVENPNWKEAGIAFIASVTRLLERLLDYRSVMQGEENRDKRMTCTVNLLNFYKNEINRKEMYLRYIYKLHNLHLQAENYTEAGFTLKLYASMLSWDRETQSFAPFDNSGQPEWQRKERLYHEILKYFDKGKCWEKGIPLCKELAHLYETRRFDYNKLSEILIQEAKFFQNILTQLRPEPEYFRVGFYGMGLPLFVRNKQFVYRGLEYERIGAFTQRLQTEFPSAQILGNNSPPDNAILNAPDQYIQISNVRPVGDAQALKTAMVPVPEKIARFYEVNDVTRFIYDRPMYKGTVDKDNEFKSLWIERTILEIASPLPGILRWYEVKQKTMQELTPVEYACEIISNAGKELSELIVQYKRDPKRNINPFSMRLQGTIDANVMGGISKYQEAFFSEQFLKSPQGAGQQANVQRLKVLILEQIQILEQALELHGQLAPSGVQPLHNRLLERFSQLKQSLSGMGRLKRQHSESIVNTPLPPLPTEQRVAPATASSSLNANAHSNYVYDLDEIYTRPGDTVRPVDPLSSYQTLSKESLSIPLEDTAAPPVPNRPRSQNFIGNGSMDSPEVPPKRQPTLNSPNAPPLPPRGITPDKRASNPLIFNDFGSGDAVGRRHSQQQQQHGQKYSVVDISYDDPEADQQPHSLPPNFQDGSGGHLSVCFAPNDFRDSGISTTSSRELNHMNLNNLSEDSSSISTITVQHREHCRISSNGSLDYNAAASMNITQRESSTSSFDVEDFPVPPPPIPPKSLAVSSNGVNFSLDEAHSPHPSTQANTTQLNHIQNPNQNHSTNSHHHLQQQQNGDGDGYSTLQHPMNGLGLPTLPPPPSAVESPMPASAIASSTSSHQHDGGTF

Target ORF information:

RefSeq Version	NM_001276119.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster sponge (spg), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276119.1

ORF Insert Sequence:

ATGTGGATAC CTTCGAACAA CAAATACGGA GTTGCCATCC ACAACTGGCA CGGCGATGTC 
CGGTTCGGCC TGGCACTGGA TGTCGGCGAT TCCGTGGACA TTGTGGAGGA GTGCAAGCAC 
TGGTACCGCG GCTCCTGCCC CCGAAAGTCG CGGGCAGTGG GTCTCTTCCC CAAGACCTAC 
ATACACATCA AGGACCTCTC GAAAATCGAT CCTGTGGTCG CCGAATGCAC GCAGGTCCTG 
CGCGAATGGT CGGAGATCTG GAAGCGTCTC TATGTGGATC GCGAAGCGTA TAAGTTCCAA 
ACCCTGCGCA AAGTTATGTT TTCCATATTG GAAAGTCGGA GGGAACTCCT TAGTGCCACC 
ATGACTCAGG ATCAAACTCT GGAGCTTCAG ATGGTTGTGG TTTCAAAGAT AGATTGGGGC 
AATCGCAAAC TCGGCCTGGA CTTGGTGCCC AGAGAGGGAC CACTGGCGGT GGATCCACAT 
GGCATTGGCA TCGTGAAGCT CTACAATGTC CACGTGAGCA GTGCTGATAA CGCCAAGGCC 
TCCTCGAGTC GGGGAACTTT GAGGCGGAAG ACGCAGCGCA AGATACTCAC CCACCACCTG 
TACTTTTGCA TGCGCGATTT TGGCCATCGG ATCGGTGGCG ATGATGCGGA GGTCTACTTC 
TTCCTCTATG ATGGGAGTCA CATGCGACCG CTGTCCGAAC GGTTTCTGGT CAAAATCTCC 
AAGGATGGTT TCTCCAATTA CATTGAAAGG TTGCACAGCA ACTGCACGGT GTTCACTGAC 
TTGGGTGCCG CTGATCTAAA TGAGGAACTT CACCTGGTGG CCGTGGTCAT GCGCGTGGGC 
AAGATCATTC AGTCGGACTC AATTAAGAAG ATCGAGAAGA GTGGCACGTT GGGTCACGGT 
CCAACCTTTC GTCGACCATT TGGAGTGGGT GTTCTCTCGC TGGGCGACAT TGCACACTTC 
GATAGCAGTC TGGAGCAGTC CTCCGATGAG CGGGAGTATA ACTTTAAGCT GTTCCAGTGC 
GAGGAGAAGG ACTTTCACCT GCTGCCCGAA CTGATTATCA AGAAATCGAG CGGGAAGTAC 
TCGCCCATCC AAGCGAGTCA GGGAACTCAG GGCATTGTTG TGTCTCTAAA ACTCCTGCAC 
GGTGGGCTGG GTCAGGCTCG GCAGGAGCAG CCGATGCTCT TCCAGGGCTC TACGATAACC 
AGGAAGATGG GCTTCCCGGA TGTCATCATG CCCGGAGATG TCCGCAACGA TCTCTTCCTC 
AATCTGGAGC GCGGTGAATT TGAGCGCGGC GGAAAGAATA CGGGAAAGAA TATCCTCGTT 
ACGGTTGTGG TGCTCGATGT GGCGGGTAAT GTTCTTACCG ACTGCCTTTG GGGTGCCTCC 
GGCATGGAAT CCCAGCCGCA GTACCGGTCC ATGATCCTAT ACCACCAGAA TGCTCCCAGT 
TGGAATGAGA TGCTGCGCCT CAGTGTGCCG ATCGACAAGT TTTCAACGGC TCATGTGCGG 
TTTGAGTTCC GACACTGTTC GACGAGGGAT AAGACGGAAC CGAAGCTATT CGCCTTCAGC 
TTTGCCAGAC TAATGGACAC CGGAGGTGCT ACTCTGGGCG ACGGCCTGCA CGAGTTGTAT 
GTGTACAAGT GCGAGGATCC TGCAAAGCTT CAGGTAGCTA ACTATCTGCG GTTGCAGTGT 
AGACCCCGCG ATGCACAGGC CAAAATGGAT TGTGGCGGTT GCTTCAGTCG CAGTTCCAAA 
GAAGTGTTCG TTCTGCGCTC CCTGCTCTGC TCCACGAAGC TGACCCAGAA CGCTGACCTT 
CTCTCGCTGC TGCAGTGGCG CACATATCCC GAAACGATCC AGGACTCGCT GACGGGAGTC 
CTTCGGCTCA ACGATGAAGA GCTGGTCAAG TTCCTGCAGG ACGTGCTGGA CGCCCTGTTT 
GCCATGTTCT CCAATGAGGA GGGTAACAGC ACCCAGCACT CGGGCCTGGT CTTCCATGTC 
CTAGTGAGCA TCTTCAGCCT GTTGCAGAGC AACAAGTTCC AACATTTTCG GCCGGTGATG 
AACGAGTACA TCGAGAATCA CTTTGCGGCT GCTTTGGTTT ACAAAGGACT CATCACCTCA 
GTGGAGCACA TGGCCGTGTT CATGACCAAG GCAGAGCACC CCGACCCATT CCAGAAGTGC 
TTCGGTTCCC TGGAGTACAT ATTCAAGTTG CTAATCCAGT CGAGGAAACT GTTTGCCAGG 
GCAACAGGTG GGCAGTATGA GGACTCGTTC AAGCGGGACC TACACTCCCT TTTTACGGCC 
CTGAATGGAA TGCTAGCTGT GCCCTCGTAC GATGTCATCA TCCCCACCCA GGAGGCCCTG 
CTTAATTCGA CGGGAGTGGT CCTGGAGCAA TTGAAGGACA CACTCCCCGC TCCGGAGTTG 
GGCATGCTGG CGCGCAATAT GCTGGACGCC ATTCCCCGGG GAGCTCCCAT TCGGTTAATT 
CAGGCAAAAT TGCATGCTGT CAAGGATCTG GTGTCTGGGG AGCTGTTCCA CGAGGATGAT 
TCCCGCACTG TGGTTCTCTC GGTGGCCTGC AAGCATCTGC GCATGCATCT AAGTCGCCGC 
GATGAACTGC GTCTGTGTGC TGAAATTCTC TCGGAGATTC TTAGCCAACT GTACGATTTA 
CAGAAGGAGC AGCGCGAGAA GGTGACCAAC ACCTTACAGC ACGATCTGGA CTCTCTGTGC 
AAAAATATAC TGGGCATTCT GATTCGTACT ATAAGCATAA TAATGGAGGG ATCCAATGCA 
GTGCTGCCGC AGTTGGTTTC CTGTCTCTTG GGCTTGCTGC AGCTGCTCGA CGAGACCCAC 
TACAAGCGCT ACTGGGACGA GCTGAGTCCC AACAAAGATC CGCGGGATCT GAAGGAGTTC 
CTGAGCAAAT CACTGCTGGT GTACGAAGAA CTGTTGACAC AGGACTGGCA TGTGTTCCCC 
AACGATTGGC TGGTAATGAA GCTTGCCGCC AACGATGTGC TGCGCATGTC GCTGGAGGAG 
TTCGCCAAGC CTCTGGTCTA CCGATTCTTG GGTCCCCAGG CATTCGATTC GCAGCTCTGG 
TGGAGCTACT TCAGTCTGGC TGTTGCCTTC TTGACACAGC CCTCCCTGCA GTTGGAGCAA 
TATAGGGAGC CCAAGAGACT GAAGATACTC CACTCGCATG GAGACATGCG GGTGCTCATG 
GGCTTCCAGA TACTCAGCAT GTGGTCGCAG CTGGGTGAGC AGAAGCTGCA CTTTATTCCA 
TCTATGGTGG GTCCATTCCT GGAGGTCACT TTGGTACCGG AGCCAGCTTT GCGGAAGGCC 
ACACTATCGG TCTTTTACGA CATGATGCAG TGCGAGCAGG CTGCTCGTGG TTCGTTCCGC 
CTGGTGGAGA GCGAACTGAT TGACAAGCTG GACCTGCTCA TCAGCGAGAA CAAGGGCGAC 
GACGAGTACA GGGAGCTGTT CAGCACCATG GAACATCTCA GCTTGGTTTT GCTGGAGAAA 
GTCCAAGTGG AGAACCCCAA CTGGAAGGAG GCTGGCATCG CTTTCATAGC CTCAGTCACC 
AGACTTTTGG AGCGCCTTCT GGACTATCGT AGCGTAATGC AGGGCGAGGA AAATCGGGAT 
AAGCGCATGA CCTGCACAGT CAATCTCCTT AATTTCTATA AAAACGAAAT CAATCGAAAG 
GAGATGTATC TGAGATACAT TTACAAGCTG CACAACCTGC ACCTGCAGGC AGAGAATTAC 
ACAGAGGCTG GCTTCACTCT GAAACTGTAT GCATCCATGC TGAGTTGGGA TCGTGAGACC 
CAGAGCTTTG CCCCCTTCGA TAATAGTGGT CAGCCAGAGT GGCAGCGCAA GGAGCGTCTA 
TACCACGAGA TCCTAAAATA CTTCGACAAG GGAAAATGTT GGGAAAAGGG CATTCCACTG 
TGCAAGGAGC TGGCTCATTT ATACGAAACG CGGCGTTTTG ACTATAACAA GTTGAGTGAA 
ATACTCATTC AGGAGGCCAA GTTCTTCCAG AATATCCTAA CTCAGTTGCG ACCGGAGCCG 
GAATACTTCC GTGTGGGATT CTACGGAATG GGTCTGCCAC TTTTTGTGCG GAACAAGCAA 
TTTGTGTACC GTGGACTGGA GTACGAGAGA ATAGGAGCCT TCACACAGCG TCTGCAAACG 
GAGTTTCCTA GTGCTCAGAT CTTGGGCAAC AACAGTCCGC CGGACAACGC CATTCTGAAT 
GCTCCGGATC AGTACATACA GATCAGCAAT GTAAGACCTG TTGGGGATGC ACAGGCCTTG 
AAGACCGCAA TGGTTCCTGT GCCGGAGAAA ATAGCTCGTT TCTACGAGGT CAACGATGTA 
ACCCGATTCA TCTACGATCG CCCCATGTAC AAGGGAACAG TTGACAAGGA CAATGAGTTC 
AAGTCGTTGT GGATAGAACG CACTATTCTA GAGATAGCTA GTCCACTGCC AGGTATTCTA 
CGCTGGTATG AGGTCAAACA GAAGACGATG CAGGAGTTAA CGCCGGTAGA GTACGCTTGC 
GAGATTATCA GCAACGCTGG CAAGGAATTA TCCGAGCTGA TTGTGCAATA TAAGCGCGAT 
CCCAAGAGGA ATATTAATCC CTTCTCCATG CGTCTGCAGG GCACAATCGA TGCTAATGTC 
ATGGGGGGTA TAAGCAAGTA CCAGGAGGCT TTCTTCTCCG AGCAGTTCCT CAAGTCGCCG 
CAAGGTGCTG GACAGCAGGC AAATGTGCAG CGGCTGAAGG TTCTGATACT GGAGCAGATT 
CAGATATTGG AGCAGGCCCT CGAACTGCAC GGTCAGCTAG CGCCCAGTGG TGTCCAGCCT 
CTGCACAATC GTCTTTTGGA ACGCTTTTCC CAGCTTAAGC AAAGTTTATC CGGCATGGGC 
AGACTGAAAC GTCAGCACTC CGAGAGCATT GTAAATACTC CCTTGCCACC GTTGCCTACA 
GAGCAGCGAG TAGCCCCGGC GACAGCTTCC TCAAGCTTGA ATGCGAATGC CCACTCCAAC 
TATGTATATG ATCTAGATGA GATCTACACG AGACCGGGAG ACACTGTACG ACCCGTGGAC 
CCACTCAGCT CCTATCAAAC ACTGAGCAAG GAGAGTCTTA GTATTCCTCT GGAGGATACT 
GCTGCTCCTC CAGTACCAAA TCGTCCTCGC TCGCAGAACT TTATTGGAAA CGGATCCATG 
GACAGCCCGG AGGTTCCGCC GAAACGACAG CCTACATTGA ATTCTCCCAA TGCTCCTCCG 
CTGCCTCCGC GGGGTATTAC ACCGGACAAG AGGGCGTCAA ATCCGTTGAT TTTCAATGAC 
TTCGGCTCCG GAGATGCCGT GGGACGTCGG CATTCTCAGC AGCAGCAGCA GCATGGCCAG 
AAGTATTCCG TTGTTGATAT TAGCTACGAT GATCCAGAGG CAGATCAGCA GCCGCATTCG 
CTGCCGCCAA ACTTCCAGGA CGGAAGCGGT GGACACCTCA GCGTCTGTTT TGCCCCGAAT 
GATTTCCGGG ACTCCGGCAT ATCGACGACT AGTTCTAGGG AACTGAACCA CATGAATCTT 
AACAACCTGA GCGAAGACTC ATCGTCGATC TCGACCATTA CAGTGCAGCA TCGAGAACAC 
TGTCGCATCA GCAGCAATGG CAGTTTGGAC TATAATGCCG CAGCATCAAT GAACATTACA 
CAACGAGAGA GTTCGACGAG TTCGTTTGAT GTGGAGGACT TTCCAGTGCC GCCGCCGCCC 
ATTCCACCCA AATCCTTGGC AGTGTCAAGC AACGGTGTCA ACTTTTCACT CGACGAGGCC 
CACTCACCGC ATCCCAGCAC ACAAGCGAAC ACCACACAAC TCAATCACAT TCAGAACCCA 
AATCAGAATC ATTCCACAAA CAGTCACCAT CATCTTCAGC AGCAGCAGAA TGGAGATGGC 
GATGGTTATA GCACGCTGCA GCACCCGATG AACGGGCTGG GGCTGCCTAC CCTTCCACCA 
CCACCTTCTG CTGTTGAATC GCCAATGCCG GCATCTGCAA TAGCCTCATC CACATCTAGT 
CATCAGCACG ATGGCGGCAC TTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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spg cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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